SCREENING OF NEW PSYCHOACTIVE SUBSTANCES IN URINE SAMPLES BY HIGH RESOLUTION MASS SPECTROMETRY

Transcription

1 SCREENING OF NEW PSYCHOACTIVE SUBSTANCES IN URINE BY HIGH RESOLUTION MASS SPECTROMETRY Noelia Salgueiro-González, Sara Castiglioni, Emma Gracia-Lor, Nikolaos I. Rousis, Lubertus Bijlsma, Alberto Celma, Félix Hernández, Ettore Zuccato

2 INDEX

3 Psychoactive Substances Natural or synthetic substances capable to affect mental proccesses when they are administrated Alcohol Drugs of abuse Hallucinogens Antidepressant drugs Nicotine Caffeine Regulation and/or outlawing

4 New Psychoactive Substances Derived from controlled drugs molecules Similar psychoactive properties of drugs of abuse High number NPS available Not regulated 2C-B (Phenethylamine) Use of NPS increased in last years High potential toxicity for human health!

5 More tan 450 NPS 150 NPS between Groups of NPS Synthetic cannabinoids Synthetic cathinones Phenethylamines Synthetic opioids Tryptamines Piperidines Aminorex derivatives Natural NPS Benzodiazepines Ketamine analogues Synthethic Cathinones Synthetic cannabinoids

6 Investigations based on NPS Epidemiological surveys Analysis of urine samples

7 Investigations based on NPS Quantitative analysis Number of NPS analyzed limited Not all standards are available Some metabolites unkwon Qualitative analysis Screening of high number of compounds/metabolites Less analysis time High Resolution mass spectrometry Promising tool for NPS identification

8 Development of instrumental methodology for the screening of NPS by liquid chromatography high resolution mass spectrometry (LC-HR-MS) Screening of the 190 selected NPS in pooled urine samples collected during music festivals Evaluation of the consumption of NPS in different European cities

9 Steps of the analytical method 5 ml of pooled urine sample Solid Phase extraction (SPE) HLB cartridges. Elution with 3 ml MeOH Determination LC-HR-MS Mobile phase: Water (0.1% formic acid) + ACN Columna: X Bridge C18, 2.1x100 mm, 3.5 µm

10 Determination LC-HR-MS Step 1: Screening Adquisition mode: Full MS Step 2: Suspected screening Adquisition mode : Full MS/ddMS n Orbitrap QExactive Software Xcalibur Step 3: Identification of suspected Adquisition mode : Full MS/DIA Confirmation of suspected: Information found in literature Analytical Standards available

11 Pooled Urine samples from European festivals Three pooled urine samples European festivals Collected from portable toilets

12 Screening of NPS Synthetic cannabinoids Synthetic cathinones Phenethylamines Synthetic opioids Tryptamines Piperidines Aminorex derivatives Natural NPS Benzodiazepines Ketamine analogues Frequency of detection reported by the European Early Warning System Software Trace Finder 190 NPS

13 Pooled urine sample. Identification and confirmation of 4-Cl-alpha-PPP (Exact mass) 4-Cl-alpha-PPP Retention time Fragments Exact mass

14 Standard 4-Cl-alpha-PPP Retention time Fragments Exact mass

15 Fragments m/z Standard (Ms) Fragments m/z sample (Mm) Error mass (Mm-Ms)/Ms Retention time agreement. Error>2% Exact mass. Error mass < 5 ppm 2 fragments identified (Error mass <5 ppm) Confirmation Complex matrix Confirmation of the presence of 4-Cl-alpha-PPP in one urine sample!!

16 The investigation of selected NPS in urine samples was carried out to evaluate the consumption of these substances in European festivals An analytical method based on high resolution mass spectrometry was proposed, being an interesting tool for the screening of NPS in urine samples Among the 190 substances investigated, only the synthetic cathinone 4-Cl-α-PPP was identified and confirmed in the analyzed samples by acquiring the corresponding analytical standard. As fas as we know, this is the first time 4-Cl-alpha-PPP was identified in urine sample from music festivals. However, further research is needed to know its concentration.

17 Postdoctoral fellowship N.Salgueiro-González Postdoctoral fellowship Holder center Research Project: HOME/2014/JDRUG/AG/DRUG/7085 Other Research projects: GRC CTM C3-2-R Conference grant N.Salgueiro-González

18 THANK YOU FOR YOUR ATTENTION!!!

19 ANY QUESTIONS? For more information Noelia Salgueiro González Postdoctoral researcher