IRIS Polymeric SPE & Accessories

Transcription

1 IRIS Polymeric SPE & Accessories Chrom Philosophy In April, a group of independent distributors met to discuss the dynamic shift in the chromatography consumables market. The group concluded that mergers and acquisitions in the chromatography consumables industry have limited the offerings available to the laboratory scientist. These users of chromatography consumables and buyers of companies from small to large are faced with limited offerings, meaning less choice and less competitive pricing. Aiming to provide more options and let local independent distributors operate on a more level playing field, the idea of a buying group was born. This buying group would enable these local distributors, who are specialized experts in chromatography, to compete with world-class quality products, yet maintaining a strong relationship with the customer. The realization of this idea is Chrom. Global companies, who purchase for many worldwide locations can benefit from our distributors by receiving group pricing and maintain the same quality with uniform pricing for all their locations. IRIS Polymeric SPE & Accessories Sorbent Properties Sorbent Selection Sorbent Mass Selection IRIS MCX IRIS WCX IRIS PLUS IRIS IRIS WAX Distributed for Chrom by: IRIS MAX Applications Sample Extraction Products General Method Guide For global contracts please contact global@chrom.com

2 IRIS Polymeric SPE Sorbents IRIS sorbents are available in six different phase chemistries offering maximum flexibility for method development. IRIS General Method Guidelines Sorbent Analyte Properties Matrix/Sample Retention Elution MIXED-MDE CATI-EXCAGE Low pka Phase IRIS PLUS % Divinylbenzene RP phase Pore size: Å Surface area: ~7 m /g IRIS Polyamide phase - LB* type sorbent Pore size: Å Surface area: ~5 m /g * Localized hydrophilic/lipophilic base IRIS MCX IRIS WCX Charged/Ionized compounds Cation-exchange (basic) analytes on-polar to moderately Aqueous diluted with buffer Example: Biological fluids igher % of aqueous solvent (water) Lower % of organic solvent Lower ionic strength polar hydrophobic eutral with buffer p < pka of analyte p > pka of sorbent MIXED-MDE AI-EXCAGE Lower % of aqueous solvent (water) igher % of organic solvent igher ionic strength p > pka of analyte p < pka of sorbent IRIS WCX rganic acid on DVB phase Pore size: Å Ion-Exchange capacity: ~1 meq/g pka ~5 Available in 5-35 µm Available in various cartridge sizes and 9-well plate formats IRIS WAX Secondary amine on DVB phase Pore size: Å Ion-Exchange capacity: ~1 meq/g pka ~7 IRIS MAX IRIS WAX Charged/Ionized compounds Anion-exchange (acidic) analytes on-polar to moderately polar hydrophobic eutral Aqueous diluted with buffer Example: Biological fluids with buffer REVERSED-PASE igher % of aqueous solvent (water) Lower % of organic solvent Lower ionic strength p > pka of analyte p < pka of sorbent Lower % of aqueous solvent (water) igher % of organic solvent igher ionic strength p < pka of analyte p > pka of sorbent igh pka Phase IRIS MCX Sulfonated hydrophilic on DVB phase Pore size: Å Ion-Exchange capacity: ~1 meq/g pka <1 IRIS MAX Quaternary amine phase on DVB phase Pore size: Å Ion-Exchange capacity: ~.1 meg/g pka >1 IRIS PLUS IRIS on-polar to moderately polar hydrophobic eutral Example: Pharmaceuticals, pesticides, herbicides, Alkaloids Aqueous diluted with buffer Example: Biological fluids, water RMAL-PASE igher % of aqueous solvent (water) Lower % of organic solvent p adjusted buffer to place analyte in neutral form (if applicable) Lower % of aqueous solvent (water) igher % of organic solvent p adjusted buffer to place analyte in charged form (if applicable) Advantages of Polymeric Solid Phase Extraction IRIS Moderate to highly polar eutral Example: Pesticides on-polar to moderately polar organic Example: Biological fluids, oil, fats igher % of organic solvent Lower % of aqueous solvent (water) p adjusted buffer to place analyte in neutral form (if applicable) Lower % of organic solvent igher % of water p adjusted buffer to place analyte in neutral form (if applicable) 1. Excellent recovery - highly retentive nature of polymeric phases. igh surface area that results in higher analyte capacity compared to silica based SPE 3. Reduced resin volumes for improved detection limits and reduced drying time. Inert to a wide array of solvents and p ranges 5. Absence of leachables. Absence of residual silanol groups 7. Resistant to dewetting This table provides general method guidelines. Each IRIS polymeric SPE box contains detailed instructions for each specific phase.

3 IRIS Sorbent Selection Guidelines Analyte Properties Strong Base? YES IRIS MCX page # IRIS WCX page # *Acid, eutral, or *Base? Retention of eutrals? STRG BALACED IRIS PLUS page # IRIS page # * If analytes contain both acids and bases, select the dominant interaction. Strong Acid? YES IRIS WAX page #1 IRIS MAX page # Sample Type and Sorbent Mass Guidelines Industry Typical Example Typical Sample Volume Recommended Sorbent Mass Recommended Reservoir Environmental Food & Agriculture Clinical & Bioanalysis Drinking water, Surface water, Waste water, Soil Food homogenate from Fish, Fruit, Meat, Poultry, Wheat Blood, Plasma, Urine diluted.1 L to 1 L mg ml 1 ml to 3 ml mg 3 ml. ml to 3 ml 3 mg 1 ml IRIS Sorbent Mass Minimum Recommended Elution Volume Typical Elution Volume 5 mg 5 ml ml to 5 ml mg ml 3 ml to ml mg µl 1. ml to 3 ml 3 mg 3 µl. ml to ml mg µl.3 ml to 1 ml * Elution volumes depend on the chemical nature of the analyte, its relative concentration in the matrix, the chemistry of the solution solvent and the sorbent bed mass. Please be advised that the tables above provide general guidelines. Please contact our technical support team for specific method development support or guidelines for specific applications. 1

4 iris plus iris plus PLUS PLUS % Divinylbenzene (DVB) phase Pore Size: Å Surface area: ~7 m /g Available in 5-35 µm particle size Available in various cartridge sizes and 9-well plate formats The IRIS PLUS is a % DVB phase. This unique polymer phase is our most retentive reversed-phase sorbent. It is a great replacement for your current C1 or PS-DVB SPE sorbents. Features & Benefits igher Recoveries due to the highly retentive nature of the DVB packing material Lower Background Signals proprietary cleaning process for ultrapure resins decreasing background signals Durability stability at p extremes of - Improved Detection Limits and Reduced Drying Time using reduced resin volumes Eliminate Matrix Effects cleaner sample extracts reduce or eliminate matrix ion suppression IRIS PLUS most hydrophobic reversed-phase available Excellent for environmental samples IRIS PLUS SPE Cartridges IRIS PLUS APPLICATI IR3 IRIS PLUS mg/1ml 5-35µm IR31 IRIS PLUS 3mg/1mL 5-35µm IR3 IRIS PLUS 3mg/3mL 5-35µm Recovery Rates: IRIS PLUS - mg/ml IR35 µg/ml in Polycyclic aromatic hydrocarbons - PA IR3 IRIS PLUS mg/3ml 5-35µm IR3 IRIS PLUS mg/3ml 5-35µm IR35 IRIS PLUS mg/ml 5-35µm 3 Chrysene Benzo(a) Anthracene Benzo(b)Fluoranthene Benzo(j)Fluoranthene Benzo(k)Fluoranthene IR3 IRIS PLUS 5mg/mL 5-35µm 3.%.5% IRIS PLUS SPE 9-well Plates IR931 IRIS PLUS 9-well mg/1ml 5-35µm 1 IR933 IRIS PLUS 9-well 3mg/1mL 5-35µm 1 Benzo(a)pyrene Benzo(e)pyrene.5% Indeno(1,,3-cd)pyrene Benzo(g,h,i)perylene Dibenz(a,h)anthracene 9.7% Group 1 Group Group 3 Group IR935 IRIS PLUS 9-well mg/1ml 5-35µm 1 IR937 IRIS PLUS 9-well 3mg/mL 5-35µm 1 IR93 IRIS PLUS 9-well mg/ml 5-35µm 1 Figure 1: GCMS Chromatogram 3

5 iris n iris n R1 R R3 Polyamide phase - LB type sorbent Pore size: Å Surface area: ~5 m /g Available in 5-35 µm particle size Available in various cartridge sizes and 9-well plate formats Features & Benefits Localized ydrophilic/lipophilic Base (LB type sorbent) using a combination of polar and non-polar interactions to provide optimal analyte/resin contact Polyamide Stationary Phase excellent water wettability using reversed-phase mode up to % aqueous solvent or normal-phase mode using up to % organic solvents This novel polyamide polymer phase provides a highly hydrophilic/lipophilic balance making it the SPE material with the widest applicability. The outstanding reversed-phase characteristics of this material are only one part of this material s success. This phase chemistry offers multiple interaction mechanisms and can be used for both reversedphase and normal-phase applications. IRIS SPE Cartridges IR IRIS mg/1ml 5-35µm IR1 IRIS 3mg/1mL 5-35µm IR IRIS 3mg/3mL 5-35µm IR IRIS mg/3ml 5-35µm IR IRIS mg/3ml 5-35µm IR5 IRIS mg/ml 5-35µm 3 IR IRIS 5mg/mL 5-35µm 3 IRIS APPLICATI IRIS - 3mg/1mL IR1 µg/ml in Recovery Rates: Estriol ydrocortisone Betamethasone Run 1 9.5% Run 1.% Run 1.3% Run.1% Run.% Run.% Run 3 9.3% Run 3 3.% Run 3.% Estriol Estriol, ydrocortisone, Betamethasone ydrocortisone IRIS SPE 9-well Plates 1 IR91 IRIS 9-well mg/1ml 5-35µm 1 IR93 IRIS 9-well 3mg/1mL 5-35µm 1 IR95 IRIS 9-well mg/1ml 5-35µm 1 IR97 IRIS 9-well 3mg/mL 5-35µm 1 IR9 IRIS 9-well mg/ml 5-35µm 1 Estriol ydrocortisone Beamethasone Betamethasone F 5

6 iris mcx iris mcx MCX MCX Sulfonated DVB phase Pore size: Å Ion-Exchange capacity: ~1 meq/g pka <1 Available in 5-35 µm particle size Available in various cartridge sizes and 9-well plate formats Features & Benefits Sulfonated DVB Phase allows for cation-exchange and reversed-phase separations % DVB no swelling, increased retention, excellent flow properties and p stability Excellent Wettability even after drying, due to hydrophilic/hydrophobic balance IRIS MCX is a water-wettable sulfonated divinylbenzene mixed-mode phase for both cation-exchange and reversedphase applications. IRIS MCX allows for easy method development, through its dual retention qualities, for a wide range of basic and neutral compounds. IRIS MCX shows excellent retention for essentially all of the common basic drugs of abuse and similar amine containing compounds. The phase is stable over the complete p range (-) and can be used with nearly any solvent, allowing the use of a wide range of solvents. IRIS MCX SPE Cartridges IR5 IRIS MCX mg/1ml 5-35µm IR51 IRIS MCX 3mg/1mL 5-35µm IR5 IRIS MCX 3mg/3mL 5-35µm IR5 IRIS MCX mg/3ml 5-35µm IR5 IRIS MCX, mg/3ml 5-35µm IR55 IRIS MCX mg/ml 5-35µm 3 IRIS MCX APPLICATI IRIS MCX - 3 mg/1ml IR51 5 µg/ml in.1% Formic Acid Recovery Rates: orephedrine Ephedrine ydrochloride ydrochloride Run 1 9.% Run 1 9.7% Run 91.% Run 9.7% Run 3 93.% Run 3 9.% orephedrine & Ephedrine ydrochloride ote 1: After wash step cartridge was dried for 1 minute using vac. The collected elute solution was placed in a ml volumetric and brought to the mark with (.1% formic acid). orephedrine ydrochloride IR5 IRIS MCX 5mg/mL 5-35µm 3 IRIS MCX SPE 9-well Plates 3 IR951 IRIS MCX 9-well mg/1ml 5-35µm 1 IR953 IRIS MCX 9-well 3mg/1mL 5-35µm orephedrine ydrochloride Ephedrine ydrochloride Ephedrine ydrochloride IR955 IRIS MCX 9-well mg/1ml 5-35µm 1 IR957 IRIS MCX 9-well 3mg/mL 5-35µm 1 3 IR95 IRIS MCX 9-well mg/ml 5-35µm 1-1 7

7 iris wcx iris wcx WCX WCX rganic acid on DVB phase Pore size: Å Ion-Exchange capacity: ~1 meq/g pka ~5 Available in 5-35 µm particle size Available in various cartridge sizes and 9-well plate formats Features & Benefits rganic Acid Phase allows for tunable selectivity for strong bases % DVB no swelling, increased retention, excellent flow properties and p stability Retain and Release easy separation of strong bases Uniquely Selective fast and efficient sample preparation and purification for strong bases IRIS WCX is a weak cation-exchange divinylbenzene polymer phase. IRIS WCX eliminates the guesswork commonly found in lengthy method development associated with strong bases such as quaternary amines. This phase utilizes the chemical functionality of a carboxylic acid. When the phase is negatively charged, it retains strong bases such as quaternary amines and releases them when neutral. IRIS WCX has repeatedly proven consistency and reliability, reducing the need for time consuming and costly retesting. IRIS WCX APPLICATI IRIS WCX SPE Cartridges IRIS WCX - 3 mg/1ml IR13 5 µg/ml in Sulfonamides IR1 IRIS WCX mg/1ml 5-35µm IR13 IRIS WCX 3mg/1mL 5-35µm IR3 IRIS WCX 3mg/3mL 5-35µm IR1 IRIS WCX mg/3ml 5-35µm Recovery Rates: Sulfadiazine Sulfathiazole Sulfamerazine Sulfamethazine Run 1 9.% Run 1 95.% Run % Run % Run.% Run 9.1% Run.5% Run 99.% Run 3 9.% Run 3 93.% Run 3 95.% Run 3 9.5% IR19 IRIS WCX mg/ml 5-35µm 3 IR IRIS WCX 5mg/mL 5-35µm 3 Sulfadiazine Sulfamerazine IR1 IRIS WCX 1gram/mL 5-35µm IRIS WCX SPE 9-well Plates 1 1 Sulfathiazole Sulfadiazine Sulfamerazine IR97 IRIS WCX 9-well mg/1ml 5-35µm 1 IR97 IRIS WCX 9-well 3mg/1mL 5-35µm 1 IR971 IRIS WCX 9-well mg/1ml 5-35µm 1 IR97 IRIS WCX 9-well 3mg/mL 5-35µm 1 Sulfathiazole 1 Sulfamethazine Sulfamethazine IR97 IRIS WCX 9-well mg/ml 5-35µm S 9

8 iris max iris max MAX MAX Quaternary amine on DVB phase Pore size: Å Ion-Exchange capacity: ~.1 meq/g pka >1 Available in 5-35 µm particle size Available in various cartridge sizes and 9-well plate formats IRIS MAX is a water-wettable quaternary amine divinylbenzene polymer phase designed for both anion-exchange and reversed-phase extraction. As a result of its bifunctionality, IRIS MAX produces outstanding retention for charged and neutral compounds. It is excellent for compounds containing an organic acid or similar ionizable functional groups. The phase is stable over the complete p range (-) and can be used with nearly any solvent permitting the use of a wide range of solvents. Features & Benefits Quaternary Amine Phase allows for anion-exchange and reversed-phase separations % DVB no swelling, increased retention, excellent flow properties and p stability Excellent Wettability even after drying, due to hydrophilic/hydrophobic balance IRIS MAX APPLICATI IRIS MAX SPE Cartridges IRIS MAX - 3 mg/1ml IR1 µg/ml in DL-Tyrosine IR IRIS MAX mg/1ml 5-35µm IR1 IRIS MAX 3mg/1mL 5-35µm IR IRIS MAX 3mg/3mL 5-35µm IR IRIS MAX mg/3ml 5-35µm Recovery Rates: Run 1 9.9% Run 9.% Run % IR IRIS MAX, mg/3ml 5-35µm IR5 IRIS MAX mg/ml 5-35µm 3 IR IRIS MAX 5mg/mL 5-35µm 3 1 IRIS MAX SPE 9-well Plates DL-Tyrosine IR91 IRIS MAX 9-well mg/1ml 5-35µm 1 IR93 IRIS MAX 9-well 3mg/1mL 5-35µm 1 IR95 IRIS MAX 9-well mg/1ml 5-35µm 1 IR97 IRIS MAX 9-well 3mg/mL 5-35µm 1 IR9 IRIS MAX 9-well mg/ml 5-35µm

9 iris wax iris wax WAX WAX Secondary amine on DVB phase Pore size: Å Ion-Exchange capacity: ~1 meq/g pka ~7 Available in 5-35 µm particle size Available in various cartridge sizes and 9-well plate formats Features & Benefits Secondary Amine Phase allows for tunable selectivity for strong acids % DVB no swelling, increased retention, excellent flow properties and p stability Excellent Retention bifunctional with reversed-phase and anion-exchange behavior Uniquely Selective fast and efficient sample preparation and purification for strong acids IRIS WAX is a mixed-mode weak anion-exchange divinylbenzene polymer phase, designed to retain and release strong acids such as sulfates. Depending on the requirements of the application, this phase can be used in the neutral or positively charged state. IRIS WAX displays both anion-exchange and reversed-phase behavior, which promotes excellent retention over a wide range of hydrophilicity for both acidic, when phase is positively charged, and neutral compounds. IRIS WAX SPE Cartridges IR11 IRIS WAX mg/1ml 5-35µm IR1 IRIS WAX 3mg/1mL 5-35µm IR9751 IRIS WAX 3mg/3mL 5-35µm IRIS WAX APPLICATI IRIS WAX - 3 mg/1ml IR1 µg/ml in Recovery Rates: itro-l-tryosine Iodo-L-Tyrosine -Acetyl-L-Tyrosine Run 1 9.7% Run 1 9.% Run % Run 9.% Run 9.% Run.% Run 3 9.% Run 3 95.% Run 3 3.% itro-l-tyrosine, Iodo-L-Tyrosine, -Acetyl-L-Tyrosine IR IRIS WAX mg/3ml 5-35µm IR1 IRIS WAX mg/ml 5-35µm 3 itro-l-tyrosine Iodo-L-Tyrosine IR19 IRIS WAX 5mg/mL 5-35µm 3 IRIS WAX SPE 9-well Plates 19 itro-l-tyrosine I IR975 IRIS WAX 9-well mg/1ml 5-35µm 1 IR975 IRIS WAX 9-well 3mg/1mL 5-35µm 1 IR9751 IRIS WAX 9-well mg/1ml 5-35µm 1 9 Iodo-L-Tyrosine -Acetyl-L-Tyrosine -Acetyl-L-Tyrosine IR975 IRIS WAX 9-well 3mg/mL 5-35µm 1 IR975 IRIS WAX 9-well mg/ml 5-35µm

10 Applications - Environmental Applications - Environmental IRIS PLUS - mg/ml IR35 µg/ml in Polycyclic aromatic hydrocarbons - PA IRIS WCX - 3 mg/1ml IR13 µg/ml in Diquat Dibromide Recovery Rates: Chrysene Benzo(a) Anthracene Benzo(b)Fluoranthene Benzo(j)Fluoranthene Benzo(k)Fluoranthene Recovery Rates: Run 1 9.% Run 9.7% Run 3 5.%.% Benzo(a)pyrene Benzo(e)pyrene.5%.5% Indeno(1,,3-cd)pyrene Benzo(g,h,i)perylene Dibenz(a,h)anthracene 9.7% Group 1 Group Group 3 Group ote: The peak area values for Diquat were found to change over time. There was a relatively quick change to lower area values that then somewhat leveled off. The area values used for quantitation were for injections of the standards and the SPE solutions in this semi-stable time frame. This observed change in peak area over time is consistent with references that state the need to deactivate glassware prior to Diquat solution contact. In this analysis the SPE Diquat solution comes in contact with three different glass vessels. Diquat is not retained by the chromatographic column, eluting in the void volume. owever, the strong absorbance at 3nm allows for quantitation without interference Diquat Dibromide 5 Figure 1: GCMS Chromatogram Figure 1: GCMS Chromatogram Br + Br IRIS - 3 mg/1ml IR1 1, 1, 5, µg/ml in Carbaryl, Atrazine, Methiocarb, Alachlor IRIS MCX - 3 mg/1ml IR51 µg/ml in,3 Dihydroxynaphthalene Recovery Rates: Carbaryl Atrazine Methiocarb Alachlor Run 1.% Run 1 9.% Run 1 1.% Run 1 9.% Run.1% Run 1.1% Run 1.% Run 1.1% Run % Run 3.% Run 3 99.% Run 3.% Recovery Rates: Run 1 9.5% Run 3.% Run 3 1.% Carbaryl Methiocarb Atrazine CI C C 3 C C 3 C Carbaryl Methiocarb Atrazine Alachlor Alachlor CI C 3 3 C S C ,3 Dihydroxynaphthalene

11 Applications - Food & Agriculture Applications - Food & Agriculture IRIS MCX - mg/ml IR55 1 mg/ml in Melamine IRIS MCX - 3 mg/1ml IR51 5 µg/ml in Carbendazim, Thiabendazole Recovery Rates: Melamine 97% Internal Standard Recovery Rates: Carbendazim Thiabendazole Run 1 1.7% Run % Run.% Run.1% Run 3.% Run 3 9.% Carbendazim Melamine 3 9 Thiabendazole Thiabendazole 19 Carbendazim 9 IRIS - 3 mg/1ml IR1 5 µg/ml in Recovery Rates: Thiamphenicol Florfenicol Chloramphenicol Run 1 9.% Run % Run % Run 99.7% Run 1.1% Run.3% Run 3 99.% Run 3.% Run 3 99.% Thiamphenicol, Florfenicol, Chloramphenicol ote 1: Elution from the IRIS SPE cartridge was performed with 7% C3C. While % C3C appeared to fully elute the compounds, the chromatographic peak shape was significantly better when the SPE elution solvent was less than % C3C. 7% was used because it was already prepared, lower C3C may lead to more improvement in chromatographic peak shape. -1 IRIS WCX - 3 mg/1ml IR13 5 µg/ml in 1 Recovery Rates: Sulfadiazine Sulfathiazole Sulfamerazine Sulfamethazine Run 1 9.% Run 1 95.% Run % Run % Run.% Run 9.1% Run.5% Run 99.% Run 3 9.% Run 3 93.% Run 3 95.% Run 3 9.5% Sulfadiazine Sulfamerazine Sulfonamides Florfenicol 35 Sulfathiazole Thiamphenicol S CI CI Thiamphenicol Florfenicol Chloramphenicol Chloramphenicol C C Sulfathiazole Sulfadiazine Sulfamerazine Sulfamethazine 13 Sulfamethazine S 1 17

12 Applications - Clinical & Bioanalysis Applications - Clinical & Bioanalysis IRIS MCX mg/ml IR55 5 µg/ml in piates Codeine IRIS - 3 mg/1ml IR1 µg/ml in Prednisone, Methylprednisolone, Dexamethasone Recovery Rates: ydrocodone 95% Dihydrocodeine 91% Methylmorphine (Codeine) 9% xycodone % ydromorphone 93% Morphine 7% xymorphone 9% ydrocodone ydrocodone ISTD Dihydrocodeine xycodone ydromorphone -acetylmophine xymorphone Morphine Morphine ISTD Recovery Rates: Prednisone Methylprednisolone Dexamethasone Run 1.7% Run % Run 1.% Run 99.5% Run 9.3% Run 9.% Run 3 99.% Run % Run % ote: Elution of the hormones from the IRIS SPE cartridge was performed with % C 3 C. It was found that lower recoveries were obtained when using % C 3 C. Methylprednisolone C 3 C 3 C C C3 Methylmorphine (Codeine) 3C ydrocodone C3 Prednisone 1 Prednisone Methylprednisolone 13 Dexamethasone Dexamethasone 3 C C 3 F 3 C C 3 Dihydrocodeine Morphine IRIS - 3 mg/1ml IR1 5 µg/ml in Recovery Rates: Risperidone % Quitiapine Fumerate 3% Domperidone 3% xymorphone -acetylmorphine Antipsychotic Drugs IRIS - 3 mg/1ml IR1 Warfarin ( µg/ml), Carbamazapine (1 µg/ml), Diphenhydramine (5 µg/ml) in Recovery Rates: Warfarin Carbamazapine Diphenhydramine Run 1 93.% Run % Run 1.% Run 95.% Run 9.% Run.1% Run 3 9.% Run 3 99.% Run 3.1% Warfarin, Carbamazapine, Diphenhydramine Carbamazapine Warfarin 9 Risperidone Warfarin Carbamazapine Diphenhydramine Diphenhydramine C 3 C 3 Domperidone Quetiapine Fumarate

13 IRIS SPE Sample Extraction Products cartridges (syringe barrel & frits) Virgin polypropylene solid phase extraction cartridges with frits Available in format ranging from 1 ml to ml 9-well plates (deep well plate format) igh throughput SBS format plates compatible with most automated systems Available with mg, 3 mg and mg bed mass online columns & guard cartridges Design for online solid phase extraction using a switching valve Wide choice of column configuration nline guard columns are available in x.1 mm & x. mm packed with 5 µm of any of the six phase chemistries (please inquire) innosep vacuum manifolds InnoSep Vacuum manifolds for SPE sample preparation, filtration, and elution are available in 1, 1, and port configurations. These manifolds permit consistent extraction and filtration results. Multiple sample processing with these manifolds simplifies procedures and saves time. The manifolds consist of a clear glass chamber and lid to which a vacuum is applied to draw a sample through an SPE column, cartridge, or disk. Adjustable racks placed in the glass vacuum chamber will accommodate a variety of sample collection vessels, including test tubes, autosampler vials, volumetric flasks, and Erlenmeyer flasks. Eluents are deposited directly into the collection vessel of choice via polypropylene, optional stainless steel, or Teflon needles.