HPLC-MALDI-TOF Mass Spectrometry for the Analysis of Complex Polymers
|
|
- Chastity King
- 6 years ago
- Views:
Transcription
1 TU Darmstadt HPLC-MALDI-TOF Mass Spectrometry for the Analysis of Complex Polymers H. Pasch German Institute for Polymers (Deutsches Kunststoff-Institut) Darmstadt, Germany 2004 Fall Workshop of the American Society for Mass Spectrometry Baltimore, December 9-10, 2004 Das DKI ist eine Einrichtung der Forschungsgesellschaft Kunststoffe e.v., Mitglied der 1 Shimadzu's Scientist Koichi Tanaka Received obel Prize in Chemistry 2002 On December 10 th, 2002, obel Prize Award Ceremony took place in Stockholm, Sweden. Koichi Tanaka, fellow of Shimadzu Corporation, received his obel medal and diploma in Chemistry from his Majesty the King of Sweden, King Carl XVI Gustaf. Tanaka received the prize for the development of soft desorption ionization methods for mass spectrometric analyses of biological macromolecules to identify and reveal the structures of such molecules. Tanaka is the 12th Japanese citizen who has received a obel Prize. 2 1
2 Why Coupling of LC and MALDI-TOF MS? Samples with high polydispersity (M w /M n >1,2) GPC calibration without calibration standards Analysis of complex polymers multiple distributions: FTD-MMD, CCD-MMD Determination of small amounts of by-products High-throughput analysis for combinatorial materials research 3 Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry 4 2
3 Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry 5 Principle Ideas of MALDI-TOF MS upon laser irradiation all molecules obtain similar energies E kin = 0.5 m v 2 at similar kinetic energies: molecules of different masses have different flight velocities (higher m means lower v) flight time in the TOF mass analyzer T ~ L (m/z U) 0.5 T - ion flight time, L - length of drift tube, m - ion mass, z - ion charge, U - acceleration voltage 6 3
4 Sample Preparation for MALDI-TOF MS Salt Matrix Solution Polymer Solution OH OH OH Deposition HO COOH OH Drying 7 Interpretation of a MALDI-TOF Spectrum -Anionically polymerized polystyrene = 82 (n) x (Styrene) + 57 (Butyl) + 1 (Hydrogen) (Ag + ) 8 4
5 Off-line Coupling of GPC and MALDI-TOF - GPC Separation of Polycaprolactone - Calibration???? ,000 g/mol 900 g/mol Off-line Coupling of GPC and MALDI-TOF - GPC Separation of Polycaprolactone - Internal calibration for more accurate molar masses 10 5
6 Off-line Coupling of LC and MALDI-TOF - Analysis of Polyethylene Oxides - Alk(Ar)-OH + CH 2 -CH 2 Alk(Ar)-(OCH 2 CH 2 ) n -OH O secondary reactions additional functionality fractions H-(OCH 2 CH 2 ) n -OH -(OCH 2 CH 2 ) n - Alk(Ar)-(OCH 2 CH 2 ) n -OAlk(Ar) 11 Off-line Coupling of LC and MALDI-TOF - Analysis of Polyethylene Oxides - Functionality type and oligomer separation 12 6
7 Off-line Coupling of LC and MALDI-TOF - Analysis of Polyethylene Oxides - Spectrum of fraction 1 GPC calibration of 13 functionality fractions Analysis of Methoxylated Melamine-Formaldehyde Resins H 2 H 2 + CH2O + CH3OH CH 3 OCH 2 CH 3 OCH 2 CH 2 OCH 3 CH 2 OCH 3 H 2 CH 3 OCH 2 CH 2 OCH 3 CH 3 OCH 2 CH 3 OCH 2 CH 2 OCH 3 CH 2 CH 3 OCH 2 OCH 2 CH 2 OCH 3 CH 2 OCH 3 CH 3 OCH 2 CH 2 OCH 3 CH 3 OCH 2 CH 2 OCH 3 14n 7
8 GPC of a Methoxylated Melamine-Formaldehyde Resin 15 MALDI-TOF Spectra of GPC Fractions of a Methoxylated Melamine-Formaldehyde Resin 16 8
9 Enlarged Tetramer Region of the MALDI-TOF Spectrum 17 Chemical Structures of the Tetramers M+a + = 1313 Da M+a + = 1343 Da M+a + = 1387 Da 18 9
10 On-line Coupled GPC/MALDI-TOF MS Injector UV- / RI-Detector Pump Sample Eluent SEC Columns 10 10:1 1 Interface 19 Schematic Presentation of a LC/MALDI-TOF Interface (Lab Connections) Eluate itrogen Heating MALDI Target X,Y-Movable Table 20 10
11 Lab Connections LC/MALDI-TOF Interface Model 600 and Pre-coated MALDI Targets 21 Analysis of PnBMA-b-PMMA by GPC/MALDI-TOF Spannung [V] PSS WinGPC scientific V 4.0 beta Elutionsvolumen [ml] 22 11
12 Analysis of PnBMA-b-PMMA by GPC/MALDI-TOF X (MMA) Y (nbma) Calc. Exp Da 3818 Da Da 3835 Da Da 3850 Da Da 3860 Da Da 3877 Da Da 3893 Da 23 LC/MALDI-TOF Interface (BAI) Liquid Chromatography SEC / CC Interface BAI Probot MALDI-TOF-MS Kratos Compact IV SEC / CC Waste 0,5 ml/min 1 40 RI- Detector Matrix 1 Waste 5 Probot MALDI- TOF-MS Data Processing Operation Data Processing 24 12
13 LC/MALDI-TOF Interface PROBOT (BAI) 25 LC-CC/MALDI-TOF Analysis of Polypropylene Oxide Triols 0.12 Spannung [V] PPG with Allylic Endgroup or Cyclic Fraction PPG Glycerol Started PPO H 2 C HC H 2 C O O O CH CH2 O CH 3 CH CH 2 O CH 3 CH CH2 O CH 3 PSS WinGPC scientific V 4.02 x y z H H H Elutionsvolumen [ml] 26 13
14 LC-CC/MALDI-TOF Analysis of Polypropylene Oxide Triols PPO Monool with Allylic Endgroup or Cyclic PPO Spannung [V] PSS WinGPC scientific V Elutionsvolumen [ml] PPG Spannung [V] PSS WinGPC scientific V Elutionsvolumen [ml] 27 LC-CC/MALDI-TOF Analysis of Polypropylene Oxide Triols Glycerol Started PPO Spannung [V] PSS WinGPC scientific V Elutionsvolumen [ml] Glycerol Started PPO Spannung [V] PSS WinGPC scientific V Elutionsvolumen [ml] 28 14
15 Direct Coupling of LC and MALDI-TOF MS Aerosol MALDI-TOF instrument coupled to GPC X. Fei, K.K. Murray, Anal. Chem. 68 (1996) Direct Coupling of LC and MALDI-TOF MS Spectra of fractions Single ion chromatograms 30 15
16 31 16
LOCALISATION, IDENTIFICATION AND SEPARATION OF MOLECULES. Gilles Frache Materials Characterization Day October 14 th 2016
LOCALISATION, IDENTIFICATION AND SEPARATION OF MOLECULES Gilles Frache Materials Characterization Day October 14 th 2016 1 MOLECULAR ANALYSES Which focus? LOCALIZATION of molecules by Mass Spectrometry
More informationNon-Traditional Approaches to MALDI- TOF Mass Spectrometry Analysis of Low Molecular Weight Polymers
Non-Traditional Approaches to MALDI- TOF Mass Spectrometry Analysis of Low Molecular Weight Polymers Justin R. Engle and S. Kim R. Williams Laboratory for Advance Separation Technologies Department of
More informationSolving practical problems. Maria Kuhtinskaja
Solving practical problems Maria Kuhtinskaja What does a mass spectrometer do? It measures mass better than any other technique. It can give information about chemical structures. What are mass measurements
More informationMatrix Assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry
Matrix Assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry Time-of-Flight Mass Spectrometry. Basic principles An attractive feature of the time-of-flight (TOF) mass spectrometer is its
More informationMALDI-TOF. Introduction. Schematic and Theory of MALDI
MALDI-TOF Proteins and peptides have been characterized by high pressure liquid chromatography (HPLC) or SDS PAGE by generating peptide maps. These peptide maps have been used as fingerprints of protein
More informationPolymer Additive Analysis by EI and APCI
Polymer Additive Analysis by EI and APCI Kate Yu, Eric Block LC/MS Waters Corporation Introduction to polymer additive analysis by LC/MS. Electron Ionization (EI) Advantages Classical spectra Library searchable
More informationMass Spectrometry Course Árpád Somogyi Chemistry and Biochemistry MassSpectrometry Facility) University of Debrecen, April 12-23, 2010
Mass Spectrometry Course Árpád Somogyi Chemistry and Biochemistry MassSpectrometry Facility) University of Debrecen, April 12-23, 2010 Introduction, Ionization Methods Mass Analyzers, Ion Activation Methods
More informationSynthesis of Sequence-Controlled Acrylate Oligomers. via Consecutive RAFT Monomers Additions
Supporting Information Synthesis of Sequence-Controlled Acrylate ligomers via Consecutive RAFT Monomers Additions Joke Vandenbergh a, Gunther Reekmans, b Peter Adriaensens b and Thomas Junkers a * a Polymer
More informationCharacterization of Synthetic Polyamides by MALDI-TOF Mass Spectrometry
2354 Bull. Korean Chem. Soc. 2007, Vol. 28, No. 12 Haeyoung Choi et al. Characterization of Synthetic Polyamides by MALDI-TOF Mass Spectrometry Haeyoung Choi, Eun Kyung Choe, Eun Kyung Yang, Sungwoo Jang,
More informationMethods in Mass Spectrometry. Dr. Noam Tal Laboratory of Mass Spectrometry School of Chemistry, Tel Aviv University
Methods in Mass Spectrometry Dr. Noam Tal Laboratory of Mass Spectrometry School of Chemistry, Tel Aviv University Sample Engineering Chemistry Biology Life Science Medicine Industry IDF / Police Sample
More informationProtein Analysis using Electrospray Ionization Mass Spectroscopy *
OpenStax-CNX module: m38341 1 Protein Analysis using Electrospray Ionization Mass Spectroscopy * Wilhelm Kienast Andrew R. Barron This work is produced by OpenStax-CNX and licensed under the Creative Commons
More informationFundamentals of Soft Ionization and MS Instrumentation
Fundamentals of Soft Ionization and MS Instrumentation Ana Varela Coelho varela@itqb.unl.pt Mass Spectrometry Lab Analytical Services Unit Index Mass spectrometers and its components Ionization methods:
More informationMALDI-TOF Mass Spectrometry: A New Rapid ID Method in Clinical Microbiology
MALDI-TOF Mass Spectrometry: A New Rapid ID Method in Clinical Microbiology Patrick R. Murray, PhD WW Director, Scientific Affairs BD Diagnostic Systems Outline MALDI-TOF is the most important innovation
More informationAnalysis of Peptides via Capillary HPLC and Fraction Collection Directly onto a MALDI Plate for Off-line Analysis by MALDI-TOF
Analysis of Peptides via Capillary HPLC and Fraction Collection Directly onto a MALDI Plate for Off-line Analysis by MALDI-TOF Application Note 219 Joan Stevens, PhD; Luke Roenneburg; Kevin Fawcett (Gilson,
More informationAutomated Sample Preparation/Concentration of Biological Samples Prior to Analysis via MALDI-TOF Mass Spectroscopy Application Note 222
Automated Sample Preparation/Concentration of Biological Samples Prior to Analysis via MALDI-TOF Mass Spectroscopy Application Note 222 Joan Stevens, Ph.D.; Luke Roenneburg; Tim Hegeman; Kevin Fawcett
More informationVitamin D3 and related compounds by ESI and APCI
Liquid Chromatography Mass Spectrometry SSI-LCMS-9 Vitamin D and related compounds by ESI and APCI LCMS-8 Summary Vitamin D and related compounds were measured by LC-ESI/APCI-MS-MS. Background Accurate
More informationMass Spectrometry Introduction
Mass Spectrometry Introduction Chem 744 Spring 2013 What MS is and is not MS is NOT a spectroscopic method. Molecules are not absorbing EM radiation MS is the generation, separation and characterization
More informationIntegration of steroids analysis in serum using LC-MS/MS with full-automated sample preparation
PO-CON69E Integration of steroids analysis in serum using LC-MS/MS with full-automated sample preparation MSACL 6 EU Stéphane Moreau, Daisuke Kawakami, Toshikazu Minohata Shimadzu Europe GmbH, Duisburg,
More informationOrganic Chemistry Laboratory Fall Lecture 3 Gas Chromatography and Mass Spectrometry June
344 Organic Chemistry Laboratory Fall 2013 Lecture 3 Gas Chromatography and Mass Spectrometry June 19 2013 Chromatography Chromatography separation of a mixture into individual components Paper, Column,
More informationSUPPLEMENTARY INFORMATION. Direct Observation of the Local Reaction Environment during the Electrochemical Reduction of CO 2
Direct Observation of the Local Reaction Environment during the Electrochemical Reduction of CO 2 Ezra L. Clark 1,2 and Alexis T. Bell* 1,2 1 Joint Center for Artificial Photosynthesis Lawrence Berkeley
More informationIonization Methods. Neutral species Charged species. Removal/addition of electron(s) Removal/addition of proton(s)
Ionization Methods Neutral species Charged species Removal/addition of electron(s) M + e - (M +. )* + 2e - electron ionization Removal/addition of proton(s) M + (Matrix)-H MH + + (Matrix) - chemical ionization
More informationCalibration Standards
Calibration Standards/ Calibration Standards TSKgel standards polystyrene TSKgel standards poly(ethylene oxide) PStQuick polystyrene calibration standards TSKgel guard column products Fittings Pre-injector
More informationBiological Mass spectrometry in Protein Chemistry
Biological Mass spectrometry in Protein Chemistry Tuula Nyman Institute of Biotechnology tuula.nyman@helsinki.fi MASS SPECTROMETRY is an analytical technique that identifies the chemical composition of
More informationAnalysis of Triglycerides in Cooking Oils Using MALDI-TOF Mass Spectrometry and Principal Component Analysis
Analysis of Triglycerides in Cooking Oils Using MALDI-TOF Mass Spectrometry and Principal Component Analysis Kevin Cooley Chemistry Supervisor: Kingsley Donkor 1. Abstract Triglycerides are composed of
More informationAutomated Purification and Analytical Reinjection of a Small Molecule Drug, Probenecid, on a Gilson LC/MS Dual Function System
Automated Purification and Analytical Reinjection of a Small Molecule Drug, Probenecid, on a Gilson LC/MS Dual Function System Keywords Introduction Application Note PHA0413 High Pressure Liquid Chromatography
More informationThe advent of soft ionization techniques such as
Comparative Studies of Poly(Dimethyl Siloxanes) Using Automated GPC-MALDI- TOF MS and On-Line GPC-ESI-TOF MS X. Michael Liu, E. Peter Maziarz, David J. Heiler, and George L. Grobe Research, Development,
More informationFully Automated Online Sample Preparation AND Quantification of Amiodarone from Whole Blood using CLAM-LC-MS/MS
PO-CON177E Fully Automated Online Sample Preparation AND Quantification of Amiodarone from Whole Blood using CLAM-LC-MS/MS ASMS 17 ThP 93 Christian Bunse 1, Lars Kröner 1 Shimadzu Deutschland GmbH, Duisburg,
More informationMass Spectrometry at the Laboratory of Food Chemistry. Edwin Bakx Laboratory of Food Chemistry Wageningen University
Mass Spectrometry at the Wageningen University Mass Spectrometry at the 3 UPLC/CE - ESI - Ion trap MS systems UPLC Thermo Acella with a Velos or VelosPro CE Beckman PA800 with a Thermo VelosPro 1 UPLC-
More informationTunable Hydrophobicity in DNA Micelles Anaya, Milena; Kwak, Minseok; Musser, Andrew J.; Muellen, Klaus; Herrmann, Andreas; Müllen, Klaus
University of Groningen Tunable Hydrophobicity in DNA Micelles Anaya, Milena; Kwak, Minseok; Musser, Andrew J.; Muellen, Klaus; Herrmann, Andreas; Müllen, Klaus Published in: Chemistry DOI: 10.1002/chem.201001816
More informationFig. 1: Chemical structure of arachidonic acid COOH CH 3
Elimination of Matrix Effects Using Mixed-mode SPE Plate for High Throughput Analysis of Free Arachidonic Acid in Plasma by LC-MS/MS Wan Wang, Suzi Qin, Linsen Li, Warren Chen, Jerry Wang 179, Southern
More informationBrooke Dilmetz. Quantitation of PFAS using MALDI-TOF MS
Brooke Dilmetz Quantitation of PFAS using MALDI-TOF MS Per and polyfluoroalkyl substances PFOS and PFOA manufactured from early 1950 s present 1 Highly persistent and bioaccumulative PFOS (Perfluorooctanesulfonate)
More informationFuranic copolymers with synthetic and natural phenolic materials for wood adhesives - a MALDI TOF study
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 DI: 10.4067/S0718-221X2015005000010 (requested) Furanic copolymers with synthetic and natural phenolic
More informationSUPPLEMENTARY INFORMATION
DOI: 10.1038/NCHEM.2419 Diversification of Self-Replicating Molecules Jan W. Sadownik, Elio Mattia, Piotr Nowak, Sijbren Otto* University of Groningen, Center for Systems Chemistry, Stratingh Institute
More informationBiological Mass Spectrometry. April 30, 2014
Biological Mass Spectrometry April 30, 2014 Mass Spectrometry Has become the method of choice for precise protein and nucleic acid mass determination in a very wide mass range peptide and nucleotide sequencing
More informationPHOTOCATALYTIC DECONTAMINATION OF CHLORANTRANILIPROLE RESIDUES IN WATER USING ZnO NANOPARTICLES. DR. A. RAMESH, Ph.D, D.Sc.,
PHOTOCATALYTIC DECONTAMINATION OF CHLORANTRANILIPROLE RESIDUES IN WATER USING ZnO NANOPARTICLES DR. A. RAMESH, Ph.D, D.Sc., raamesh_a@yahoo.co.in 1 OBJECTIVES Determination of persistence and photolysis
More informationSupporting information for
Supporting information for Nitrogen and Sulfur Co-doped Carbon Dots-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Imaging for Profiling Bisphenol S Distribution in Mouse Tissues
More informationRemoval of Triton X-100 from Plasma Samples Using Mixed-Mode Solid Phase Extraction (SPE)
Removal of Triton X- from Plasma Samples Using Mixed-Mode Solid Phase Extraction (SPE) Jonathan P. Danaceau, Erin Chambers, and Kenneth J. Fountain Waters Corporation, 34 Maple Street, Milford, MA USA
More informationTime-of-Flight LC/MS Identification and Confirmation of a Kairomone in Daphnia magna Cultured Medium. Application. Authors. Abstract.
Time-of-Flight LC/MS Identification and Confirmation of a Kairomone in Daphnia magna Cultured Medium Application Natural Product Chemistry Authors Hideaki Uchida Agilent Technologies Japan, Ltd. Tokyo
More information[ APPLICATION NOTE ] Profiling Mono and Disaccharides in Milk and Infant Formula Using the ACQUITY Arc System and ACQUITY QDa Detector
Profiling Mono and Disaccharides in Milk and Infant Formula Using the ACQUITY Arc System and ACQUITY QDa Detector Mark Benvenuti, Gareth Cleland, and Jennifer Burgess Waters Corporation, Milford, MA, USA
More informationDetection of Low Level of Chloramphenicol in Milk and Honey with MIP SPE and LC-MS-MS
Detection of Low Level of Chloramphenicol in Milk and Honey with MIP SPE and LC-MS-MS Olga Shimelis, An Trinh, and Michael Ye Supelco, Div. of Sigma-Aldrich, Bellefonte, PA T407125 Introduction Molecularly
More informationSmall Molecule Science: Experimental designs for achieving ultra trace analysis
Small Molecule Science: Experimental designs for achieving ultra trace analysis Michael P. Balogh Principal scientist Waters Corporation 211 Waters Corporation 1 www.cosmoscience.org The Society for Small
More informationAn optical dosimeter for the selective detection of gaseous phosgene with ultra-low detection limit
Supporting information for An optical dosimeter for the selective detection of gaseous phosgene with ultra-low detection limit Alejandro P. Vargas, Francisco Gámez*, Javier Roales, Tània Lopes-Costa and
More informationThe study of phospholipids in single cells using an integrated microfluidic device
Supporting Information: The study of phospholipids in single cells using an integrated microfluidic device combined with matrix-assisted laser desorption/ionization mass spectrometry Weiyi Xie,, Dan Gao,
More informationCharacterization of Noncovalent Complexes of Polyelectrolytes by Mass Spectrometry
The University of Akron IdeaExchange@UAkron Honors Research Projects The Dr. Gary B. and Pamela S. Williams Honors College Spring 2015 Characterization of Noncovalent Complexes of Polyelectrolytes by Mass
More informationChapter 12: Mass Spectrometry: molecular weight of the sample
Structure Determination: hapter 12: Mass Spectrometry- molecular weight of the sample; formula hapter 12: Infrared Spectroscopy- indicated which functional groups are present hapter 13: Nuclear Magnetic
More informationAgilent 7700x ICP-MS
Agilent 7700x ICP-MS Metals analysis Mid ppt to low ppb detection range Option to detect in four different gas modes to gain optimum detection of multiple metals or interferences in a sample Autosampler
More informationIonization Methods. Ionization Methods
Ionization Methods Árpád Somogyi Associate Director CCIC, Mass Spectrometry and Proteomics Laboratory OSU August 17, 2015 Ionization Methods Neutral species Charged species Removal/addition of electron(s)
More informationDetermination of glycerol derivatives by High-performance liquid chromatography
Determination of glycerol derivatives by High-performance liquid chromatography JUAN CARLOS BELTRÁN PRIETO a, JIŘÍ PECHA a, VĚRA KAŠPÁRKOVÁ b,c, KAREL KOLOMAZNÍK a a Department of Automation and Control
More informationMatrix-assisted laser desorption ionization
FOCUS: SYNTHETIC POLYMERS Analysis of the Accuracy of Determining Average Molecular Weights of Narrow Polydispersity Polymers by Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry
More informationAnalytical Chemistry News & Features July 1, 1998; pp. 456 A-461 A. Copyright 1998 American Chemical Society
1 of 13 Original Article located at: http://pubs.acs.org/hotartcl/ac/98/jul/report.html Analytical Chemistry News & Features July 1, 1998; pp. 456 A-461 A. Copyright 1998 American Chemical Society Characterizing
More informationHigh-Throughput Analysis of Oligonucleotides using Automated Electrospray Ionization Mass Spectrometry
High-Throughput Analysis of Oligonucleotides using Automated Electrospray Ionization Mass Spectrometry Mark E. Hail 1, Brian Elliott 2, Kerry Nugent 3, Jeffrey L. Whitney 1, and David J. Detlefsen 1 1
More informationComponents of a Mass Spectrometer
Components of a Mass Spectrometer Sample Introduction Inlet GC LC Direct Insertion (Syringe/Probe) Ionization Ion Separation Ion Detection Ion Source EI,CI,,, MALDI Mass Analyzer Under vacuum TOF, Quadrupole,
More information1. Sample Introduction to MS Systems:
MS Overview: 9.10.08 1. Sample Introduction to MS Systems:...2 1.1. Chromatography Interfaces:...3 1.2. Electron impact: Used mainly in Protein MS hard ionization source...4 1.3. Electrospray Ioniztion:
More informationMass spectrometry Technologies in Lipid chemistry
Mass spectrometry Technologies in Lipid chemistry Rabah Soliymani University Of Helsinki Protein Chemistry Unit Biomedicum Helsinki Rabah.soliymani@helsinki.fi Complex_&_dynamic_mixtures (few copies to
More informationRESEARCH ARTICLE. Surface Assisted Laser Desorption Ionization- Mass Spectrometry (SALDI-MS) for Analysis of Polyester Degradation Products
B American Society for Mass Spectrometry, 2012 J. Am. Soc. Mass Spectrom. (2012) 23:1071Y1076 DOI: 10.1007/s13361-012-0360-8 RESEARCH ARTICLE Surface Assisted Laser Desorption Ionization- Mass Spectrometry
More informationSeparation of Polyphenols by Comprehensive 2D-LC and Molecular Formula Determination by Coupling to Accurate Mass Measurement
Separation of Polyphenols by Comprehensive 2D-LC and Molecular Formula Determination by Coupling to Accurate Mass Measurement Application Note Food Testing & Agriculture Author Edgar Naegele Agilent Technologies,
More informationIntroduction to Proteomics 1.0
Introduction to Proteomics 1.0 CMSP Workshop Pratik Jagtap Managing Director, CMSP Objectives Why are we here? For participants: Learn basics of MS-based proteomics Learn what s necessary for success using
More informationFundamental Studies of Matrix-Assisted Laser Desorption/Ionization, Using Time-of-Flight Mass Spectrometry to Identify Biological Molecules
UCRL-ID-126246 Fundamental Studies of Matrix-Assisted Laser Desorption/Ionization, Using Time-of-Flight Mass Spectrometry to Identify Biological Molecules D. Eades, D. Wruck, H. Gregg November 11, 1996
More informationUltra Performance Liquid Chromatography Coupled to Orthogonal Quadrupole TOF MS(MS) for Metabolite Identification
22 SEPARATION SCIENCE REDEFINED MAY 2005 Ultra Performance Liquid Chromatography Coupled to Orthogonal Quadrupole TOF MS(MS) for Metabolite Identification In the drug discovery process the detection and
More informationLC/MS Method for Comprehensive Analysis of Plasma Lipids
Application Note omics LC/MS Method for Comprehensive Analysis of Plasma s Authors Tomas Cajka and Oliver Fiehn West Coast Metabolomics Center, University of California Davis, 451 Health Sciences Drive,
More informationType Analysis of Citrus Essential Oils by Multidimensional Supercritical Fluid Chromatography/ Gas Chromatography
25 Type Analysis of Citrus Essential Oils by Multidimensional Supercritical Fluid Chromatography/ Gas Chromatography Takashi YARITA, Akira NoMURA and Yoshiyuki HoRIMOTo Department of Analytical Chemistry,
More informationSupporting information
Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 2014 Supporting information Glycan Reductive Isotope-coded Amino Acid Labeling (GRIAL) for Mass Spectrometry-based
More informationAnalysis of polymer molecular weight distributions
Hydrocarbon Polymer Analysis by External MALDI Fourier Transform and Reflectron Time of Flight Mass Spectrometry Arwah J. Jaber and Charles L. Wilkins Department of Chemistry and Biochemistry, University
More informationSUPPORTING INFORMATION:
1S SUPPORTING INFORMATION: The discovery of the once weekly glucagon like peptide 1 (GLP-1) analog semaglutide Jesper Lau*, Paw Bloch, Lauge Schäffer, Ingrid Pettersson, Jane Spetzler, Jacob Kofoed, Kjeld
More informationCannabinoid Profiling and Quantitation in Hemp Extracts using the Agilent 1290 Infinity II/6230B LC/TOF system
Cannabinoid Profiling and Quantitation in Hemp Extracts using the Agilent 9 Infinity II/63B LC/TOF system Application Brief Authors Mike Adams, Karen Kaikaris, and A. Roth CWC Labs Joan Stevens, Sue D
More informationANALYTICAL REPORT 1 3C-P (C14H23NO3) 1-(3,5-dimethoxy-4-propoxylphenyl)propan-2-amine.
Vodovodna 95, 1000 LJUBLJANA, SLOVENIA T: +386 (0)1 428 44 93 E: nfl@policija.si www.policija.si Remark other NPS detected: none ANALYTICAL REPORT 1 3C-P (C14H23NO3) 1-(3,5-dimethoxy-4-propoxylphenyl)propan-2-amine
More informationThe Detection of Allergens in Food Products with LC-MS
The Detection of Allergens in Food Products with LC-MS Something for the future? Jacqueline van der Wielen Scope of Organisation Dutch Food and Consumer Product Safety Authority: Law enforcement Control
More informationANALYTICAL REPORT 1. bk-2c-b ( C10H12BrNO3) 2-amino-1-(4-bromo-2,5-dimethoxyphenyl)ethan-1-one
Vodovodna 95 1000 Ljubljana SLOVENIJA T: +386 (0)1 428 44 93 E: nfl@policija.si www.policija.si Remark other NPS detected: none ANALYTICAL REPORT 1 bk-2c-b ( C10H12BrNO3) 2-amino-1-(4-bromo-2,5-dimethoxyphenyl)ethan-1-one
More informationANALYTICAL REPORT 1. N-methyl-2AI ( C10H13N) Remark other NPS detected: none. N-methyl-2,3-dihydro-1H-inden-2-amine. Sample ID:
Vodovodna 95 1000 Ljubljana SLOVENIJA T: +386 (0)1 428 44 93 E: nfl@policija.si www.policija.si Remark other NPS detected: none ANALYTICAL REPORT 1 N-methyl-2AI ( C10H13N) N-methyl-2,3-dihydro-1H-inden-2-amine
More informationAnalysis of small organics on planar silica surfaces using surface-assisted laser desorption/ionization mass spectrometry
RAPID COMMUNICATIONS IN MASS SPECTROMETRY Rapid Commun. Mass Spectrom. 2001; 15: 1899±1903 Analysis of small organics on planar silica surfaces using surface-assisted laser desorption/ionization mass spectrometry
More information2D-LC as an Automated Desalting Tool for MSD Analysis
2D-LC as an Automated Desalting Tool for MSD Analysis Direct Mass Selective Detection of a Pharmaceutical Peptide from an MS-Incompatible USP Method Application Note Biologics and Biosimilars Author Sonja
More informationMass Spectrometry. Actual Instrumentation
Mass Spectrometry Actual Instrumentation August 2017 See also http://www.uni-bielefeld.de/chemie/analytik/ms f additional infmation 1. MALDI TOF MASS SPECTROMETRY ON THE ULTRAFLEX 2 2. ESI MASS SPECTROMETRY
More informationA NOVEL METHOD OF M/Z DRIFT CORRECTION FOR OA-TOF MASS SPECTROMETERS BASED ON CONSTRUCTION OF LIBRARIES OF MATRIX COMPONENTS.
A NOVEL METHOD OF M/Z DRIFT CORRECTION FOR OA-TOF MASS SPECTROMETERS BASED ON CONSTRUCTION OF LIBRARIES OF MATRIX COMPONENTS. Martin R Green*, Keith Richardson, John Chipperfield, Nick Tomczyk, Martin
More informationFast Separation of Triacylglycerols in Oils using UltraPerformance Convergence Chromatography (UPC 2 )
Fast Separation of Triacylglycerols in Oils using UltraPerformance Convergence Chromatography (UPC 2 ) Mehdi Ashraf-Khorassani, 1 Larry T. Taylor, 1 Jinchuan Yang, 2 and Giorgis Isaac 2 1 Department of
More informationRobust and Fast Analysis of Tobacco-Specific Nitrosamines by LC-MS/MS
Application Note 242 Robust and Fast Analysis of Tobacco-Specific Nitrosamines by LC-MS/MS INTRODUCTION Tobacco-specific nitrosamines (TSNA) are a group of carcinogens found only in tobacco products. They
More informationSupporting Information for. Interaction of Spin-Labeled HPMA-based Nanoparticles with Human Blood Plasma Proteins
Electronic Supplementary Material (ESI) for Nanoscale. This journal is The Royal Society of Chemistry 2018 Supporting Information for Interaction of Spin-Labeled HPMA-based Nanoparticles with Human Blood
More information[ Care and Use Manual ]
MALDI Calibration Kit I. Introduction The MALDI Calibration Kit is a conveniently packaged selection of MALDI matrices and calibration standards (includes high-purity Neg Ion Mode Calibrant for accurate
More informationSeparation of 15 Underivatized Saccharide and Sialic Acid USP Standards
Application Note Raw Material & Media Analysis Separation of Underivatized Saccharide and Sialic Acid USP Standards Using an Agilent AdvanceBio MS Spent Media Column with TOF MS Detection Author Richard
More informationKoji URAKAMI,*,a Chiaki KOBAYASHI, a Yoshihiko MIYAZAKI, a Koji NISHIJIMA, a Yoshinobu YOSHIMURA, a and Keiji HASHIMOTO b
September 2000 Chem. Pharm. Bull. 48(9) 1299 1303 (2000) 1299 Degradation Products Generated by Sonication of Benzyl Alcohol, a Sample Preparation Solvent for the Determination of Residual Solvents in
More informationDetermination of Amantadine Residues in Chicken by LCMS-8040
Liquid Chromatography Mass Spectrometry Determination of Amantadine Residues in Chicken by LCMS-8040 A method for the determination of amantadine in chicken was established using Shimadzu Triple Quadrupole
More informationANALYTICAL REPORT 1 DPT (C16H24N2) [2-(1H-indol-3-yl)ethyl]dipropylamine.
Vodovodna 95 1000 Ljubljana SLOVENIJA T: +386 (0)1 428 44 93 E: nfl@policija.si www.policija.si Remark other NPS detected: none ANALYTICAL REPORT 1 DPT (C16H24N2) [2-(1H-indol-3-yl)ethyl]dipropylamine
More informationQuantitative Analysis of Carbohydrates and Artificial Sweeteners in Food Samples Using GFC- MS with APCI Interface and Post-column Reagent Addition
PO-CON1321E Quantitative Analysis of Carbohydrates and Artificial Sweeteners in Food Samples Using GFC- MS with APCI Interface and Post-column Reagent Addition ASMS 213 TP 71 Jie Xing 1, Yin Ling Chew*
More informationANALYTICAL REPORT 5F-MDMB-PINACA (C20H28FN3O3) methyl 2-{[1-(5-fluoropentyl)-1H-indazol-3-yl]formamido}-3,3-dimethylbutanoate
Vodovodna 95 1000 Ljubljana SLOVENIJA ANALYTICAL REPORT 5F-MDMB-PINACA (C20H28FN3O3) methyl 2-{[1-(5-fluoropentyl)-1H-indazol-3-yl]formamido}-3,3-dimethylbutanoate Remark other NPS detected: none Sample
More informationFigure S7: ATR FTIR spectra of enantiomeric UP-PLA-UPy and sc-upy-pla-upy. Figure S9: SEM microphotographs sc-upy-pla-oh in 1,4-dioxane and chloroform
Supporting Information for Macromolecules, DOI: Supramolecular Polylactides by the Cooperative Interaction of the End Groups and Stereocomplexation By M. Brzeziński*, T. Biela Table of Contents Figure
More informationFuture of Metabolomics. Issues in metabolomics research
Knowledge that will change your world Ist UAB Metabolomics Workshop July 22 25, 2013 Future of Metabolomics Stephen Barnes, PhD Director, Targeted Metabolomics and Proteomics Laboratory Issues in metabolomics
More informationUltra High Definition Optimizing all Analytical Dimensions
Ultra High Definition Optimizing all Analytical Dimensions Sensitivity Dynamic Range Signal Response Linearity Separation Speed Peak Capacity Chromatogram Mass Spectrum Mass Accuracy Resolving Power Acquisition
More informationUltra High Performance Liquid Chromatograph. Nexera X2 C196-E079
Ultra High Performance Liquid Chromatograph Nexera X2 C196-E79 Maximizing the Potential of UHPLC/HPLC Analyses Intelligence Intelligent data solutions, including a new i -PDeA peak calculation method for
More informationProbing for Packaging Migrants in a Pharmaceutical Impurities Assay Using UHPLC with UV and Mass Detection INTRODUCTION
Probing for Packaging Migrants in a Pharmaceutical Impurities Assay Using UHPLC with UV and Mass Detection Michael Jones Waters Corporation, Wilmslow, UK APPLICATION BENEFITS The ACQUITY Arc System is
More informationSupporting information for: Regioselective cross metathesis for block and heterotelechelic polymer synthesis
Electronic Supplementary Material (ESI) for Polymer Chemistry. This journal is The Royal Society of Chemistry 2016 Supporting information for: Regioselective cross metathesis for block and heterotelechelic
More informationANALYTICAL REPORT 1, 2
Vodovodna 95 1000 Ljubljana SLOVENIJA T: +386 (0)1 428 44 93 E: nfl@policija.si www.policija.si Remark other NPS detected: none ANALYTICAL REPORT 1, 2 4-MEAP (C14H21NO) 2-(ethylamino)-1-(4-methylphenyl)pentan-1-one
More informationLipids Analysis. Lipids
Lipids Analysis Stephen Barnes 3 5 15 Lipids Lipids are mostly very hydrophobic Most are conjugates of fatty acids of a variety of chain lengths, which have different degrees of unsaturation, cis trans
More informationNCUT National Centre for Upgrading Technology
NCUT National Centre for Upgrading Technology a Canada Alberta alliance for bitumen and heavy oil research Comparison of the Reactivity of Naphthenic Acids in Athabasca bitumen and San Joaquin Valley Parviz
More informationTrans-stereospecific polymerization of butadiene and random copolymerization with styrene using borohydrido rare earths / magnesium dialkyl catalysts
Trans-stereospecific polymerization of butadiene and random copolymerization with styrene using borohydrido rare earths / magnesium dialkyl catalysts A. Ventura a, T. Chenal b,c,d,e, *, M. Bria b,f, F.
More informationANALYTICAL REPORT 1, 2
Vodovodna 95 1000 Ljubljana SLOVENIJA T: +386 (0)1 428 44 93 E: nfl@policija.si www.policija.si Remark other NPS detected: none ANALYTICAL REPORT 1, 2 Phenibut (C10H13NO2) 4-amino-3-phenylbutanoic acid
More informationImpurity Identification using a Quadrupole - Time of Flight Mass Spectrometer QTOF
Impurity Identification using a Quadrupole - Time of Flight Mass Spectrometer QTOF PUSHER TOF DETECTOR ZSPRAY TM Ion Source SAMPLING CONE SKIMMER RF HEXAPOLE RF HEXAPOLE QUADRUPOLE IN NARROW BANDPASS MODE
More informationHypersil BDS and Hypersil Classical HPLC Columns
LC Columns and Accessories Hypersil BDS and Hypersil Classical HPLC Columns Exceptionally reliable and reproducible general purpose columns Exceptionally reliable robust columns that guarantee you have
More informationPolymer characterization by combining liquid chromatography with MALDI and ESI mass spectrometry
Anal Bioanal Chem (2002) 373 : 481 489 DOI 10.1007/s00216-002-1332-9 REVIEW Renata Murgasova David M. Hercules Polymer characterization by combining liquid chromatography with MALDI and ESI mass spectrometry
More informationNeosolaniol. [Methods listed in the Feed Analysis Standards]
Neosolaniol [Methods listed in the Feed Analysis Standards] 1 Simultaneous analysis of mycotoxins by liquid chromatography/ tandem mass spectrometry [Feed Analysis Standards, Chapter 5, Section 1 9.1 ]
More informationAnnamária Jakab. Supervisor: Eszter Forgács
ABSTRACT F THE DCTRAL THESIS CHARACTERIZATIN F PLANT ILS BASED N THEIR TRIACYLGLYCERL CNTENT BY HPLC/APCI-MS AND MALDI-TFMS Annamária Jakab Supervisor: Eszter Forgács Research Laboratory of Materials and
More informationIon Source. Mass Analyzer. Detector. intensity. mass/charge
Proteomics Informatics Overview of spectrometry (Week 2) Ion Source Analyzer Detector Peptide Fragmentation Ion Source Analyzer 1 Fragmentation Analyzer 2 Detector b y Liquid Chromatography (LC)-MS/MS
More information