Mass Spectrometry at the Laboratory of Food Chemistry. Edwin Bakx Laboratory of Food Chemistry Wageningen University
|
|
- Victoria Richards
- 5 years ago
- Views:
Transcription
1 Mass Spectrometry at the Wageningen University
2 Mass Spectrometry at the 3 UPLC/CE - ESI - Ion trap MS systems UPLC Thermo Acella with a Velos or VelosPro CE Beckman PA800 with a Thermo VelosPro 1 UPLC- ESI Ion mobility TOF MS system Waters Acquity H Class with Synapt G2Si 1 Prep HPLC Single quad MS Waters AutoPurifier system 1 MALDI-TOF/TOF MS Bruker - UltraFlextreme 1 Thermo DSQ Thermo - DSQ
3 Bruker UltraFlextreme MALDI-TOF/TOF MS Location: Axis-X Room L1-01 gfqrd Contact Person Henk Schols Daily responsible
4 Bruker UltraFlextreme MALDI-TOF/TOF MS Matrix Assisted Laser Desorption Ionization Time Of Flight Resolution (FWHM) (Reflector mode) Mass Accuracy < 2 ppm (internal calibration, Reflector mode) TOF/TOF Post Source Decay (PSD) and Collision Induced Dissociation (CID) Simple sample preparation Extreme fast measurement No separation in front
5 Bruker UltraFlextreme Principle of MALDI-TOF/TOF-MS
6 Bruker UltraFlextreme Applications "standard" Proteomics Identification of glycopeptides/proteins Identification of phosphopeptides/proteins Analysing intact proteins (up to 150 kda) Sequencing of peptides and proteins (DeNovo Sequencing) Profiling and identification of charged and neutral oligosaccharides Identification of phytonutrients (Saponins, Polyphenols etc) Identification of bacteria (with BioTyper)
7 ] Bruker UltraFlextreme Identification of oligosaccharides in complex mixtures In te n s. [a.u.] x H H H18 H H5 H H8 H H10 H _Auto_random4 0:N1 MS Raw Maltodextrine and Carboxymethyl inulin In te n s. [a.u.] Carboxymethyl inulin _Auto_random4 0:N5 MS Raw m/z m/z H = Hexose
8 Bruker UltraFlextreme Identification of oligosaccharides in complex mixtures Intens. [a.u.] MALDI-TOF/TOF-MS of CMI 4 11 (m/z = ) 1000 Z/Y 3 Z/Y 2 Z/Y Main structure B/C 1 B/C 2 B/C 3 Z/Y 3 Z/Y 2 Z/Y B/C 1 B/C 2 B/C carboxymethyl group m/z m/z
9 Bruker UltraFlextreme Identification of chemical modifications of proteins Intens. [a.u.] [M+H] + alac blg L M MTT :J22 MS, Smoothed 1000 [2M+H] + [3M+H] Native = Da [4M+H] After 48 h = Da 250 Intens. [a.u.] alac blg L M MTT :J23 MS, Smoothed Difference = 1137 Da Hexose = 162 Da Nr of hexose attached to protein m/z
10 Waters Acquity & Synapt G2 HDMS UPLC-ESI-TWIM-MS Location: Axis-X Room L1-07 Contact Person Henk Schols Daily responsible
11 Waters Acquity & Synapt G2Si HDMS Synapt G2Si HDMS Electrospray ionisation (ESI) Inlet: Direct infusion or UPLC Electron transfer dissociation (ETD) Travelling Wave Ion Mobility (TWIM) IM resolution 40 Time of Flight detection (TOF) Resolution (FWHM) Mass Accuracy < 1 ppm (Lock mass)
12 Waters Acquity & Synapt G2Si HDMS Acquity H Class Bio UPLC system Flow rate ml /min Quaternary pump system Bio compatible (titanium) Detection Photo diode array ( nm) Fluorescence Synapt G2Si HDMS with ETD
13 Traveling-Wave Ion Mobility Mass Spectrometry
14 Waters Acquity & Synapt G2Si HDMS Applications "standard" Proteomics Identification and quantification of glycopeptides /proteins Identification and quantification of phosphopeptides/ proteins Analysing intact proteins and modifications on intact proteins (up to 25 kda) Profiling, identification and quantification of charged and neutral oligosaccharides Identification of isomeric oligosaccharides Identification and quantification of phytonutrients (Saponins, Polyphenols etc)
15 Waters Acquity & Synapt G2Si HDMS Identification of (isomeric) oligosaccharides in a complex sample EXAMPLE: 3-AQ labelled methyl esterified galacturonic oligomers from pectin digest % Possible structures m/z Peak label 3 1 = 3 GalA + 1 Methyl
16 EXAMPLE : 3-AQ labelled methyl esterified galacturonic oligomers Ion mobility profile
17 Waters Acquity & Synapt G2Si HDMS Identification of the chemical modifications of proteins isoforms a-lactalbumin Glycosylated a-lactalbumin Mw = 162 = HEXOSE
18 Waters Autopurication LC-Prep Mass Directed auto purification system Location: Axis-X Room L1-07 Contact Person Henk Schols Daily responsible Mark Sanders
19 Waters AutoPurification system Waters Mass directed Auto Purification LC-Prep Preparative HPLC pump Quaternary pump (max. flow rate 150 ml/min) Injection At Column dilution Detectors Photo Diode Array (PDA) nm Single Quad MS ESI and APCI interface Full scan m/z Optional: Evaporate Light Scattering (ELS) Fraction collector Up to 448 test tubes
20 Waters AutoPurification system Waters Mass directed Auto Purification LC-Prep The configuration enables easy scale-up from: Analytical chromatography (micrograms) Semi-preparative chromatography (milligrams) Preparative chromatography (gram quantities)
21 Waters AutoPurification system Mass directed Auto Purification
22 Waters AutoPurification system Applications Mass directed purification of Primary metabolites Phytonutrients and a broad range of other secondary metabolites Oligosaccharides (Bio-active) peptides
23 CAT-AgroFood MS at the Remarks A broad range of MS Apparatus present at FCH The MALDI and the Synapt are complementary systems The Prep HPLC is unique fractionation system
Agilent 7700x ICP-MS
Agilent 7700x ICP-MS Metals analysis Mid ppt to low ppb detection range Option to detect in four different gas modes to gain optimum detection of multiple metals or interferences in a sample Autosampler
More information2. Ionization Sources 3. Mass Analyzers 4. Tandem Mass Spectrometry
Dr. Sanjeeva Srivastava 1. Fundamental of Mass Spectrometry Role of MS and basic concepts 2. Ionization Sources 3. Mass Analyzers 4. Tandem Mass Spectrometry 2 1 MS basic concepts Mass spectrometry - technique
More information1. Sample Introduction to MS Systems:
MS Overview: 9.10.08 1. Sample Introduction to MS Systems:...2 1.1. Chromatography Interfaces:...3 1.2. Electron impact: Used mainly in Protein MS hard ionization source...4 1.3. Electrospray Ioniztion:
More informationImpurity Identification using a Quadrupole - Time of Flight Mass Spectrometer QTOF
Impurity Identification using a Quadrupole - Time of Flight Mass Spectrometer QTOF PUSHER TOF DETECTOR ZSPRAY TM Ion Source SAMPLING CONE SKIMMER RF HEXAPOLE RF HEXAPOLE QUADRUPOLE IN NARROW BANDPASS MODE
More informationSCS Mass Spectrometry Laboratory
SCS Mass Spectrometry Laboratory Contact Information Staff 31 Noyes Laboratory (8:00-5:00 M-F) 217-333-2545 http://scs.illinois.edu/massspec/ Furong Sun (frs@illinois.edu) Furong Sun Director Training
More informationMethods in Mass Spectrometry. Dr. Noam Tal Laboratory of Mass Spectrometry School of Chemistry, Tel Aviv University
Methods in Mass Spectrometry Dr. Noam Tal Laboratory of Mass Spectrometry School of Chemistry, Tel Aviv University Sample Engineering Chemistry Biology Life Science Medicine Industry IDF / Police Sample
More informationComparison of mass spectrometers performances
Comparison of mass spectrometers performances Instrument Mass Mass Sensitivity resolution accuracy Quadrupole 1 x 10 3 0.1 Da* 0.5-1.0 pmol DE-MALDI 2 x 10 4 20 ppm 1-10 fmol peptide 1-5 pmol protein Ion
More informationMALDI-TOF. Introduction. Schematic and Theory of MALDI
MALDI-TOF Proteins and peptides have been characterized by high pressure liquid chromatography (HPLC) or SDS PAGE by generating peptide maps. These peptide maps have been used as fingerprints of protein
More informationLOCALISATION, IDENTIFICATION AND SEPARATION OF MOLECULES. Gilles Frache Materials Characterization Day October 14 th 2016
LOCALISATION, IDENTIFICATION AND SEPARATION OF MOLECULES Gilles Frache Materials Characterization Day October 14 th 2016 1 MOLECULAR ANALYSES Which focus? LOCALIZATION of molecules by Mass Spectrometry
More informationNew Solvent Grade Targeted for Trace Analysis by UHPLC-MS
New Solvent Grade Targeted for Trace Analysis by UHPLC-MS Subhra Bhattacharya, Deva H. Puranam, and Stephen C. Roemer Thermo Fisher Scientific Fisher Chemical, One Reagent Lane, Fair Lawn, NJ Material
More informationAnalysis of Pesticides (II) Metribuzin & their metabolites in Rice Jun Yonekubo, Nihon Waters, JAPAN
Waters Alliance LC/MS System Analytical Conditions Chromatograms Mass Spectra Calibration Quantitation Analysis of Pesticides (II) Metribuzin & their metabolites in Rice Jun Yonekubo,, JAPAN Key Words
More informationIon Source. Mass Analyzer. Detector. intensity. mass/charge
Proteomics Informatics Overview of spectrometry (Week 2) Ion Source Analyzer Detector Peptide Fragmentation Ion Source Analyzer 1 Fragmentation Analyzer 2 Detector b y Liquid Chromatography (LC)-MS/MS
More informationBiological Mass Spectrometry. April 30, 2014
Biological Mass Spectrometry April 30, 2014 Mass Spectrometry Has become the method of choice for precise protein and nucleic acid mass determination in a very wide mass range peptide and nucleotide sequencing
More informationFundamentals of Soft Ionization and MS Instrumentation
Fundamentals of Soft Ionization and MS Instrumentation Ana Varela Coelho varela@itqb.unl.pt Mass Spectrometry Lab Analytical Services Unit Index Mass spectrometers and its components Ionization methods:
More informationApplying a Novel Glycan Tagging Reagent, RapiFluor-MS, and an Integrated UPLC-FLR/QTof MS System for Low Abundant N-Glycan Analysis
Applying a Novel Glycan Tagging Reagent, RapiFluor-MS, and an Integrated UPLC-FLR/QTof MS System for Low Abundant N-Glycan Analysis Ying Qing Yu Waters Corporation, Milford, MA, USA APPLICATION BENEFITS
More informationMass Spectrometry. Actual Instrumentation
Mass Spectrometry Actual Instrumentation August 2017 See also http://www.uni-bielefeld.de/chemie/analytik/ms f additional infmation 1. MALDI TOF MASS SPECTROMETRY ON THE ULTRAFLEX 2 2. ESI MASS SPECTROMETRY
More informationBiological Mass spectrometry in Protein Chemistry
Biological Mass spectrometry in Protein Chemistry Tuula Nyman Institute of Biotechnology tuula.nyman@helsinki.fi MASS SPECTROMETRY is an analytical technique that identifies the chemical composition of
More informationPolymer Additive Analysis by EI and APCI
Polymer Additive Analysis by EI and APCI Kate Yu, Eric Block LC/MS Waters Corporation Introduction to polymer additive analysis by LC/MS. Electron Ionization (EI) Advantages Classical spectra Library searchable
More informationMass Spectrometry. - Introduction - Ion sources & sample introduction - Mass analyzers - Basics of biomolecule MS - Applications
- Introduction - Ion sources & sample introduction - Mass analyzers - Basics of biomolecule MS - Applications Adapted from Mass Spectrometry in Biotechnology Gary Siuzdak,, Academic Press 1996 1 Introduction
More informationA NOVEL METHOD OF M/Z DRIFT CORRECTION FOR OA-TOF MASS SPECTROMETERS BASED ON CONSTRUCTION OF LIBRARIES OF MATRIX COMPONENTS.
A NOVEL METHOD OF M/Z DRIFT CORRECTION FOR OA-TOF MASS SPECTROMETERS BASED ON CONSTRUCTION OF LIBRARIES OF MATRIX COMPONENTS. Martin R Green*, Keith Richardson, John Chipperfield, Nick Tomczyk, Martin
More information[ APPLICATION NOTE ] APPLICATION BENEFITS INTRODUCTION WATERS SOLUTIONS KEYWORDS
[ APPLICATI TE ] Ion Mobility-enabled Data-dependent Experiments Distinguishing Co-eluting Isomeric Metabolites Using an IMS-QTof Mass Spectrometer Jayne Kirk, 1 Russell Mortishire Smith, 1 Robert Beecher,
More informationIntroduction to Proteomics 1.0
Introduction to Proteomics 1.0 CMSP Workshop Pratik Jagtap Managing Director, CMSP Objectives Why are we here? For participants: Learn basics of MS-based proteomics Learn what s necessary for success using
More informationIonization Methods. Neutral species Charged species. Removal/addition of electron(s) Removal/addition of proton(s)
Ionization Methods Neutral species Charged species Removal/addition of electron(s) M + e - (M +. )* + 2e - electron ionization Removal/addition of proton(s) M + (Matrix)-H MH + + (Matrix) - chemical ionization
More informationIsomer Separation of Positively Labeled N-glycans by CE-ESI-MS
Isomer Separation of Positively Labeled N-glycans by CE-ESI-MS G.S.M. Kammeijer Center for Proteomics and Metabolomics CE IN THE BIOTECHNOLOGY & PHARMACEUTICAL INDUSTRIES 19 TH SYMPOSIUM ON THE PRACTICAL
More informationSolving practical problems. Maria Kuhtinskaja
Solving practical problems Maria Kuhtinskaja What does a mass spectrometer do? It measures mass better than any other technique. It can give information about chemical structures. What are mass measurements
More informationCharacterization of an Unknown Compound Using the LTQ Orbitrap
Characterization of an Unknown Compound Using the LTQ rbitrap Donald Daley, Russell Scammell, Argenta Discovery Limited, 8/9 Spire Green Centre, Flex Meadow, Harlow, Essex, CM19 5TR, UK bjectives unknown
More informationTargeted and untargeted metabolic profiling by incorporating scanning FAIMS into LC-MS. Kayleigh Arthur
Targeted and untargeted metabolic profiling by incorporating scanning FAIMS into LC-MS Kayleigh Arthur K.Arthur@lboro.ac.uk Introduction LC-MS is a highly used technique for untargeted profiling analyses
More informationDon t miss a thing on your peptide mapping journey How to get full coverage peptide maps using high resolution accurate mass spectrometry
Don t miss a thing on your peptide mapping journey How to get full coverage peptide maps using high resolution accurate mass spectrometry Kai Scheffler, PhD BioPharma Support Expert,LSMS Europe The world
More informationSingle Quadrupole Compact Mass Spectrometer for Liquid Sample
Single Quadrupole Compact Mass Spectrometer for Liquid Sample YL INSTRUMENT CO., LTD. YL9900 MS quickly provides essential information and improves the chemist's workflow. Mordern organic labs have become
More informationNew Instruments and Services
New Instruments and Services Liwen Zhang Mass Spectrometry and Proteomics Facility The Ohio State University Summer Workshop 2016 Thermo Orbitrap Fusion http://planetorbitrap.com/orbitrap fusion Thermo
More informationAB Sciex QStar XL. AIMS Instrumentation & Sample Report Documentation. chemistry
Mass Spectrometry Laboratory AIMS Instrumentation & Sample Report Documentation AB Sciex QStar XL chemistry UNIVERSITY OF TORONTO AIMS Mass Spectrometry Laboratory Department of Chemistry, University of
More informationSYNAPT G2-S High Definition MS (HDMS) System
SYNAPT G2-S High Definition MS (HDMS) System High performance, versatility, and workflow efficiency of your MS system all play a crucial role in your ability to successfully reach your scientific and business
More informationMass Spectrometry Course Árpád Somogyi Chemistry and Biochemistry MassSpectrometry Facility) University of Debrecen, April 12-23, 2010
Mass Spectrometry Course Árpád Somogyi Chemistry and Biochemistry MassSpectrometry Facility) University of Debrecen, April 12-23, 2010 Introduction, Ionization Methods Mass Analyzers, Ion Activation Methods
More informationMass spectra of peptides and proteins - and LC analysis of proteomes Stephen Barnes, PhD
Mass spectra of peptides and proteins - and LC analysis of proteomes Stephen Barnes, PhD 4-7117 sbarnes@uab.edu Overview A mass spectrum Electrospray MS Analysis of intact proteins Molecular weight calculations
More informationComponents of a Mass Spectrometer
Components of a Mass Spectrometer Sample Introduction Inlet GC LC Direct Insertion (Syringe/Probe) Ionization Ion Separation Ion Detection Ion Source EI,CI,,, MALDI Mass Analyzer Under vacuum TOF, Quadrupole,
More informationAutomated Sample Preparation/Concentration of Biological Samples Prior to Analysis via MALDI-TOF Mass Spectroscopy Application Note 222
Automated Sample Preparation/Concentration of Biological Samples Prior to Analysis via MALDI-TOF Mass Spectroscopy Application Note 222 Joan Stevens, Ph.D.; Luke Roenneburg; Tim Hegeman; Kevin Fawcett
More informationSystematic analysis of protein-detergent complexes applying dynamic light scattering to optimize solutions for crystallization trials
Supporting information 1 2 3 Volume 71 (2015) Supporting information for article: 4 5 6 7 8 Systematic analysis of protein-detergent complexes applying dynamic light scattering to optimize solutions for
More informationAnalysis of N-Linked Glycans from Coagulation Factor IX, Recombinant and Plasma Derived, Using HILIC UPLC/FLR/QTof MS
Analysis of N-Linked Glycans from Coagulation Factor IX, Recombinant and Plasma Derived, Using HILIC UPLC/FLR/QTof MS Ying Qing Yu Waters Corporation, Milford, MA, U.S. A P P L I C AT ION B E N E F I T
More informationMALDI Imaging Drug Imaging Detlev Suckau Head of R&D MALDI Bruker Daltonik GmbH. December 19,
MALDI Imaging Drug Imaging Detlev Suckau Head of R&D MALDI Bruker Daltonik GmbH December 19, 2014 1 The principle of MALDI imaging Spatially resolved mass spectra are recorded Each mass signal represents
More informationProfiling Flavonoid Isomers in Highly Complex Citrus Juice Samples Using UPLC Ion Mobility Time-of-Flight Mass Spectrometry
Profiling Flavonoid Isomers in Highly Complex Citrus Juice Samples Using UPLC Ion Mobility Time-of-Flight Mass Spectrometry Michael McCullagh, 1 Kieran Neeson, 2 and Antonietta Gledhill 1 Waters Corporation,
More informationMass spectrometry Technologies in Lipid chemistry
Mass spectrometry Technologies in Lipid chemistry Rabah Soliymani University Of Helsinki Protein Chemistry Unit Biomedicum Helsinki Rabah.soliymani@helsinki.fi Complex_&_dynamic_mixtures (few copies to
More informationSUPPORTING INFORMATION:
1S SUPPORTING INFORMATION: The discovery of the once weekly glucagon like peptide 1 (GLP-1) analog semaglutide Jesper Lau*, Paw Bloch, Lauge Schäffer, Ingrid Pettersson, Jane Spetzler, Jacob Kofoed, Kjeld
More informationCONTENT. i iv ix. SVKM s NMIMS, School of Pharmacy and Technology Management
CONTENT Chapter No. Title Page No. Abbreviations List of Figures List of Tables 1 Introduction 1 1.1 Practical aspects 4 1.2 Stability-indicating assay method (SIAM) 5 1.3 Regulatory aspects 6 1.4 Techniques
More informationNew Instruments and Services
New Instruments and Services http://planetorbitrap.com/orbitrap fusion Combining the best of quadrupole, Orbitrap, and ion trap mass analysis in a revolutionary Tribrid architecture, the Orbitrap Fusion
More informationApplication Note # ET-17 / MT-99 Characterization of the N-glycosylation Pattern of Antibodies by ESI - and MALDI mass spectrometry
Bruker Daltonics Application Note # ET-17 / MT-99 Characterization of the N-glycosylation Pattern of Antibodies by ESI - and MALDI mass spectrometry Abstract Analysis of the N-glycosylation pattern on
More informationOMCL Network of the Council of Europe QUALITY MANAGEMENT DOCUMENT
OMCL Network of the Council of Europe QUALITY MANAGEMENT DOCUMENT PA/PH/OMCL (10) 86 2R QUALIFICATION OF EQUIPMENT ANNEX 7: QUALIFICATION OF MASS SPECTROMETERS Full document title and reference Document
More informationMass-Based Purification of Natural Product Impurities Using an Agilent 1260 Infinity II Preparative LC/MSD System
Application Note Food Testing and Agriculture Mass-Based Purification of Natural Product Impurities Using an Agilent 126 Infinity II Preparative LC/MSD System Authors Florian Rieck and Jörg Hippler Agilent
More informationMore structural information with MS n
PRODUCT SPECIFICATIONS The LTQ XL linear ion trap mass spectrometer More structural information with MS n The LTQ XL linear ion trap mass spectrometer delivers more structural information faster and with
More informationTechnical Note # TN-31 Redefining MALDI-TOF/TOF Performance
Bruker Daltonics Technical Note # TN-31 Redefining MALDI-TOF/TOF Performance The new ultraflextreme exceeds all current expectations of MALDI-TOF/TOF technology: A proprietary khz smartbeam-ii TM MALDI
More informationHigh-Throughput Analysis of Oligonucleotides using Automated Electrospray Ionization Mass Spectrometry
High-Throughput Analysis of Oligonucleotides using Automated Electrospray Ionization Mass Spectrometry Mark E. Hail 1, Brian Elliott 2, Kerry Nugent 3, Jeffrey L. Whitney 1, and David J. Detlefsen 1 1
More informationProteomics of body liquids as a source for potential methods for medical diagnostics Prof. Dr. Evgeny Nikolaev
Proteomics of body liquids as a source for potential methods for medical diagnostics Prof. Dr. Evgeny Nikolaev Institute for Biochemical Physics, Rus. Acad. Sci., Moscow, Russia. Institute for Energy Problems
More informationMetabolomics: quantifying the phenotype
Metabolomics: quantifying the phenotype Metabolomics Promises Quantitative Phenotyping What can happen GENOME What appears to be happening Bioinformatics TRANSCRIPTOME What makes it happen PROTEOME Systems
More informationSupporting information
Supporting information Figure legends Supplementary Table 1. Specific product ions obtained from fragmentation of lithium adducts in the positive ion mode comparing the different positional isomers of
More informationIon fragmentation of small molecules in mass spectrometry
Ion fragmentation of small molecules in mass spectrometry Jeevan Prasain jprasain@uab.edu 6-2612 Nomenclature: the main names and acronyms used in mass spectrometry Molecular ion: Ion formed by addition
More information[ APPLICATION NOTE ] High Sensitivity Intact Monoclonal Antibody (mab) HRMS Quantification APPLICATION BENEFITS INTRODUCTION WATERS SOLUTIONS KEYWORDS
Yun Wang Alelyunas, Henry Shion, Mark Wrona Waters Corporation, Milford, MA, USA APPLICATION BENEFITS mab LC-MS method which enables users to achieve highly sensitive bioanalysis of intact trastuzumab
More informationMatrix Assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry
Matrix Assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry Time-of-Flight Mass Spectrometry. Basic principles An attractive feature of the time-of-flight (TOF) mass spectrometer is its
More informationQuadrupole and Ion Trap Mass Analysers and an introduction to Resolution
Quadrupole and Ion Trap Mass Analysers and an introduction to Resolution A simple definition of a Mass Spectrometer A Mass Spectrometer is an analytical instrument that can separate charged molecules according
More informationMass Spectrometry. Mass spectrometer MALDI-TOF ESI/MS/MS. Basic components. Ionization source Mass analyzer Detector
Mass Spectrometry MALDI-TOF ESI/MS/MS Mass spectrometer Basic components Ionization source Mass analyzer Detector 1 Principles of Mass Spectrometry Proteins are separated by mass to charge ratio (limit
More informationSummary Chapter 8 CHAPTER 8. Summary. Page 173
CHAPTER 8 Summary Page 173 Chapter 2: Liquid Chromatography/Tandem Mass Spectrometry Method for Quantitative Estimation of PEG 400 and its Applications A rapid sensitive and selective MRM based method
More informationAdvantages of Ion Mobility Q-TOF for Characterization of Diverse Biological Molecules
Advantages of Ion Mobility Q-TOF for Characterization of Diverse Biological Molecules Add a New Dimension to your Research Capability with Agilent s New Drift Ion Mobility Q-TOF System Overview: 6560 IM
More informationThe Comparison of High Resolution MS with Triple Quadrupole MS for the Analysis of Oligonucleotides
The Comparison of High Resolution MS with Triple Quadrupole MS for the Analysis of Oligonucleotides Mohammed Abrar Unilabs York Bioanalytical Solutions Outline Introduction Why LC-MS/MS? Limitations of
More informationData Independent MALDI Imaging HDMS E for Visualization and Identification of Lipids Directly from a Single Tissue Section
Data Independent MALDI Imaging HDMS E for Visualization and Identification of Lipids Directly from a Single Tissue Section Emmanuelle Claude, Mark Towers, and Kieran Neeson Waters Corporation, Manchester,
More informationMass Spectrometry Infrastructure
Mass Spectrometry Infrastructure Todd Williams, Ph.D. Director KU Mass Spectrometry and Analytical Proteomics Laboratory Mass Spectrometry Lab B025 Malott Hall Mission The Mass Spectrometry and analytical
More informationEdgar Naegele. Abstract
Simultaneous determination of metabolic stability and identification of buspirone metabolites using multiple column fast LC/TOF mass spectrometry Application ote Edgar aegele Abstract A recent trend in
More informationSmall Scale Preparative Isolation of Corticosteroid Degradation Products Using Mass-Based Fraction Collection Application
Small Scale Preparative Isolation of Corticosteroid Degradation Products Using Mass-Based Fraction Collection Application Pharmaceutical Author Cliff Woodward Agilent Technologies, Inc. 285 Centerville
More informationAutomated Purification and Analytical Reinjection of a Small Molecule Drug, Probenecid, on a Gilson LC/MS Dual Function System
Automated Purification and Analytical Reinjection of a Small Molecule Drug, Probenecid, on a Gilson LC/MS Dual Function System Keywords Introduction Application Note PHA0413 High Pressure Liquid Chromatography
More informationO O H. Robert S. Plumb and Paul D. Rainville Waters Corporation, Milford, MA, U.S. INTRODUCTION EXPERIMENTAL. LC /MS conditions
Simplifying Qual/Quan Analysis in Discovery DMPK using UPLC and Xevo TQ MS Robert S. Plumb and Paul D. Rainville Waters Corporation, Milford, MA, U.S. INTRODUCTION The determination of the drug metabolism
More informationCannabinoid Profiling and Quantitation in Hemp Extracts using the Agilent 1290 Infinity II/6230B LC/TOF system
Cannabinoid Profiling and Quantitation in Hemp Extracts using the Agilent 9 Infinity II/63B LC/TOF system Application Brief Authors Mike Adams, Karen Kaikaris, and A. Roth CWC Labs Joan Stevens, Sue D
More informationPHOTOCATALYTIC DECONTAMINATION OF CHLORANTRANILIPROLE RESIDUES IN WATER USING ZnO NANOPARTICLES. DR. A. RAMESH, Ph.D, D.Sc.,
PHOTOCATALYTIC DECONTAMINATION OF CHLORANTRANILIPROLE RESIDUES IN WATER USING ZnO NANOPARTICLES DR. A. RAMESH, Ph.D, D.Sc., raamesh_a@yahoo.co.in 1 OBJECTIVES Determination of persistence and photolysis
More informationAn Alternative Approach: Top-Down Bioanalysis of Intact Large Molecules Can this be part of the future? Lecture 8, Page 27
An Alternative Approach: Top-Down Bioanalysis of Intact Large Molecules Can this be part of the future? Lecture 8, Page 27 Top-down HRAM Bioanalysis of Native Proteins/Molecules Relative Abundance 100
More information[ APPLICATION NOTE ] Profiling Mono and Disaccharides in Milk and Infant Formula Using the ACQUITY Arc System and ACQUITY QDa Detector
Profiling Mono and Disaccharides in Milk and Infant Formula Using the ACQUITY Arc System and ACQUITY QDa Detector Mark Benvenuti, Gareth Cleland, and Jennifer Burgess Waters Corporation, Milford, MA, USA
More informationApplication Note # LCMS-89 High quantification efficiency in plasma targeted proteomics with a full-capability discovery Q-TOF platform
Application Note # LCMS-89 High quantification efficiency in plasma targeted proteomics with a full-capability discovery Q-TOF platform Abstract Targeted proteomics for biomarker verification/validation
More informationSmall Molecule Science: Experimental designs for achieving ultra trace analysis
Small Molecule Science: Experimental designs for achieving ultra trace analysis Michael P. Balogh Principal scientist Waters Corporation 211 Waters Corporation 1 www.cosmoscience.org The Society for Small
More informationAn Introduction. 1 Introduction MALDI-TOF/TOF_version100623
MALDI-TOF/TOF mass spectrometry An Introduction 1 Introduction MALDI-TOF/TOF_version100623 MALDI-TOF(/TOF) mass spectrometry MALDI ion source Time-of of-flight (TOF) mass analyzer detector 2 Introduction
More informationMALDI Imaging Mass Spectrometry
MALDI Imaging Mass Spectrometry Nan Kleinholz Mass Spectrometry and Proteomics Facility The Ohio State University Mass Spectrometry and Proteomics Workshop What is MALDI Imaging? MALDI: Matrix Assisted
More informationNature Biotechnology: doi: /nbt Supplementary Figure 1
Supplementary Figure 1 The timeline of the NGAG method for extraction of N-linked glycans and glycosite-containing peptides. The timeline can be changed based on the number of samples. Supplementary Figure
More informationReducing Sample Volume and Increasing Sensitivity for the Quantification of Human Insulin and 5 Analogs in Human Plasma Using ionkey/ms
Reducing Sample Volume and Increasing Sensitivity for the Quantification of Human Insulin and 5 Analogs in Human Plasma Using ionkey/ms Erin E. Chambers and Kenneth J. Fountain Waters Corporation, Milford,
More informationBioanalytical Quantitation of Biotherapeutics Using Intact Protein vs. Proteolytic Peptides by LC-HR/AM on a Q Exactive MS
Bioanalytical Quantitation of Biotherapeutics Using Intact Protein vs. Proteolytic Peptides by LC-HR/AM on a Q Exactive MS Jenny Chen, Hongxia Wang, Zhiqi Hao, Patrick Bennett, and Greg Kilby Thermo Fisher
More informationMS/MS as an LC Detector for the Screening of Drugs and Their Metabolites in Race Horse Urine
Application Note: 346 MS/MS as an LC Detector for the Screening of Drugs and Their Metabolites in Race Horse Urine Gargi Choudhary and Diane Cho, Thermo Fisher Scientific, San Jose, CA Wayne Skinner and
More informationHigh Resolution Glycopeptide Mapping of EPO Using an Agilent AdvanceBio Peptide Mapping Column
High Resolution Glycopeptide Mapping of EPO Using an Agilent AdvanceBio Peptide Mapping Column Application Note BioPharma Authors James Martosella, Phu Duong, and Alex Zhu Agilent Technologies, Inc. Abstract
More informationSimple Method (IS-MRM) to Monitor Lysophospholipids and Phospholipids During LC-MS Method Development via In-Source CID
Simple Method (IS-MRM) to Monitor Lysophospholipids and Phospholipids During LC-MS Method Development via In-Source CID James Little, Eastman Chemical Company, Kingsport, TN Overview Phospholipids and
More informationMASS SPECTROMETRIC CHARACTERIZATION OF ELASTIN PEPTIDES AND THE EFFECT OF SOLAR RADIATION ON ELASTIN. Dissertation
MASS SPECTROMETRIC CHARACTERIZATION OF ELASTIN PEPTIDES AND THE EFFECT OF SOLAR RADIATION ON ELASTIN Dissertation zur Erlangung des akademischen Grades Doktor rerum naturalium (Dr. rer. nat.) vorgelegt
More informationAnalysis of HMF by HPLC
COST Action 927 Training School Building Skills on the Analysis of Thermal Process Contaminants in Foods 22-26 October 2007, Ankara Analysis of HMF by HPLC Vural Gökmen O O OH Background O COOH O R 2 Carbonyl
More informationApplication of LC/Electrospray Ion Trap Mass Spectrometry for Identification and Quantification of Pesticides in Complex Matrices
Application ote #LCMS-2 esquire series Application of LC/Electrospray Ion Trap Mass Spectrometry for Identification and Quantification of Pesticides in Complex Matrices Introduction The simple monitoring
More informationAnalytical Challenges in Veterinary Toxicology: Bromethalin
Analytical Challenges in Veterinary Toxicology: Bromethalin Mike Filigenzi, Birgit Puschner, Linda Aston, Robert Poppenga CAHFS-UC Davis School of Veterinary Medicine First things first: Acknowledgements
More informationUPLC-MS/MS Analysis of Azole Antifungals in Serum for Clinical Research
Stephen Balloch and Gareth Hammond Waters Corporation, Wilmslow, UK APPLICATION BENEFITS Analytical selectivity afforded by mass selective detection Wide linear measuring range Simple, inexpensive sample
More informationDr. Erin E. Chambers Waters Corporation. Presented by Dr. Diego Rodriguez Cabaleiro Waters Europe Waters Corporation 1
Development of an SPE-LC/MS/MS Assay for the Simultaneous Quantification of Amyloid Beta Peptides in Cerebrospinal Fluid in Support of Alzheimer s Research Dr. Erin E. Chambers Waters Corporation Presented
More informationBruker Daltonics. autoflex III smartbeam. The Standard in MALDI-TOF Performance MALDI-TOF/TOF. think forward
Bruker Daltonics autoflex III smartbeam The Standard in MALDI-TOF Performance think forward MALDI-TOF/TOF Designed for a Routine High Level of Performance The autoflex III smartbeam brings the power of
More informationHigh Throughput Accurate Mass Screening of Monoclonal Antibodies
High Throughput Accurate Mass Screening of Monoclonal Antibodies Jim Blasberg April 28, 211 sigma-aldrich.com Life Science and High Technology Center Life Science and High Technology Center2 Sigma-Aldrich,
More informationIdentification of Steroids in Water by Ion Trap LC/MS/MS Application
Identification of Steroids in Water by Ion Trap LC/MS/MS Application Environmental Author Paul Zavitsanos and Christine Miller Agilent Technologies, Inc. Centerville Road Wilmington, DE 9- USA Abstract
More informationAdvancing your Forensic Toxicology Analyses; Adopting the Latest in Mass Spectrometry Innovations
Advancing your Forensic Toxicology Analyses; Adopting the Latest in Mass Spectrometry Innovations For Research Use Only. Not for use in diagnostic procedures. 1 2015 AB Sciex RUO-MKT-11-1018-A For research
More informationAdvances in Hybrid Mass Spectrometry
The world leader in serving science Advances in Hybrid Mass Spectrometry ESAC 2008 Claire Dauly Field Marketing Specialist, Proteomics New hybrids instruments LTQ Orbitrap XL with ETD MALDI LTQ Orbitrap
More informationAnalysis of Peptides via Capillary HPLC and Fraction Collection Directly onto a MALDI Plate for Off-line Analysis by MALDI-TOF
Analysis of Peptides via Capillary HPLC and Fraction Collection Directly onto a MALDI Plate for Off-line Analysis by MALDI-TOF Application Note 219 Joan Stevens, PhD; Luke Roenneburg; Kevin Fawcett (Gilson,
More informationMass Spectrometry and Proteomics - Lecture 1 - Matthias Trost Newcastle University
Mass Spectrometry and Proteomics - Lecture 1 - Matthias Trost Newcastle University matthias.trost@ncl.ac.uk Content Lectures 1-3 The basics of mass measurement Ionisation techniques Mass analysers Detectors
More informationIntroduction to LC/MS/MS
Trends in 2006 Introduction to LC/MS/MS By Crystal Holt, LC/MS Product Specialist, Varian Inc. Toxicology laboratories Increased use of LC/MS Excellent LD Cheaper (still expensive) Much more robust Solves
More informationUsing Software Tools to Improve the Detection of Impurities by LC/MS. Application Note. Christine Miller Agilent Technologies.
Using Software Tools to Improve the Detection of Impurities Application Note Christine Miller Introduction The analysis of raw materials and finished products for or impurities presents a challenge in
More informationMeasuring Lipid Composition LC-MS/MS
Project: Measuring Lipid Composition LC-MS/MS Verification of expected lipid composition in nanomedical controlled release systems by liquid chromatography tandem mass spectrometry AUTHORED BY: DATE: Sven
More informationNew Developments in LC-IMS-MS Proteomic Measurements and Informatic Analyses
New Developments in LC-IMS-MS Proteomic Measurements and Informatic Analyses Erin Shammel Baker Kristin E. Burnum-Johnson, Xing Zhang, Cameron P. Casey, Yehia M. Ibrahim, Matthew E. Monroe, Tao Liu, Brendan
More informationComprehensive Two-Dimensional HPLC and Informative Data Processing for Pharmaceuticals and Lipids
PO-CON1576E Comprehensive Two-Dimensional HPLC and Informative Data Processing for Pharmaceuticals and Lipids HPLC 2015 PSB-MULTI-06 Yoshiyuki WATABE, Tetsuo IIDA, Daisuke NAKAYAMA, Kanya TSUJII, Saki
More informationSample Preparation is Key
PLOS ONE DOI: 10.1371/journal.pone.0117232 February 6, 2015 Presented by Katie Gibbs Sample Preparation is Key Sample extraction and instrumental analysis methods are well documented in metabolomics. Understanding
More information