Fatty Acid Applications by GC
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1 Fatty Acid Applications by GC INDEX Olive oil...1 Canola oil...2 Sesame oil...2 Potato chips...3 DHA supplement...3 Tuna(chu-toro)...4 Tuna(minced)...4 Butter...5 Tween ,6 Tween Tween Tween Tween Pig fat...8 Wild boar fat...9 Milk...9 Sardine...10 Soybean oil...10 Potato chips...11 Green leaf of tobacco...11 Egg yolk...12 BSA...12,13 Unpolished rice...13 Yeast...14 Linseed oil...14 Safflower oil...15 Rice bran...15 Soybean oil (partially-hydrolyzed)..16 Toasted soybean flour...16 Japanese horse mackerel...17 E. coli...17 Blood...18 Bovine heart...18 Bovine liver...19 Bovine brain...19 Plasma...20 Milk...20 Egg yolk...21 Ice cream...21 Mayonnaise...22 Mustard...22 Wasabi paste...23 Miso...23 Butter...24 Powdered milk(for 0-1 year old)...24 Powdered milk(for 1-3 years old)...25 Human milk...25 Fatty Acid Methylation Kits...26 Ordering Information...27 Olive oil 1
2 Canola oil Sesame oil 2
3 Potato chips DHA supplement 3
4 Tuna(chu-toro) Tuna(minced) 4
5 Butter Tween 20 5
6 Tween 20 Tween 40 6
7 Tween 60 Tween 80 7
8 Tween 85 Pig fat 8
9 Wild boar fat Milk 9
10 Sardine Soybean oil 10
11 Potato chips Green leaf of tobacco 11
12 Egg yolk BSA 12
13 BSA Unpolished rice 13
14 Yeast Linseed oil 14
15 Safflower oil Rice bran 15
16 Soybean oil (partially-hydrolyzed) Toasted soybean flour 16
17 Japanese horse mackerel E. coli 17
18 Blood Bovine heart 18
19 Bovine liver Bovine brain 19
20 Plasma Milk 20
21 Egg yolk Ice cream 21
22 Mayonnaise Mustard 22
23 Wasabi paste Miso 23
24 Butter Powdered milk(for 0-1 year old) 24
25 Powdered milk(for 1-3 years old) Human milk 25
26 Methyl esterification kit for fatty acids analysis Fatty Acid Methylation Kits Below are two methods for efficiently preparing fatty acid samples for GC analysis. Neither method requires high temperatures, unlike the conventional method, so methylation can be done safely and easily. Targeted fatty acids Targeted samples Fatty Acid Methylation Kit Glycerides (glycerolipids, such as triglycerides, diglycerides, monoglycerides and lecithin) Free fatty acids Sterol esters Blood, yeast, liver, cooking oil, soybean powder, fish oils Fatty Acid Methylation Kit for Glycerides Glycerides (glycerolipids, such as triglycerides, diglycerides, monoglycerides and lecithin) Glycerides containing short-chain fatty acids Cooking oil, fish oils Sample Methylation Reagent A 0.5 ml Methylation Reagent B 0.5 ml, 37 o C, 1 hr Methylation Reagent C 0.5 ml, 37 o C, 20 min Isolation Reagent 1.0 ml Upper layer Deionized Water 1.0 ml Upper layer Sample Reagent A (solvent) 1.0 ml Reagent B (reaction solution) 0.1 ml; room temp, stir 3 sec, let stand 10 sec Reagent C (stop solution) 1.0 ml Upper layer GC Analysis Purification with Fatty Acid Methyl Ester Purification Kit * If using a packed column, this step is not required. Procedure GC Analysis Conventional Method Sample Extraction of fatty acids React at 37oC Simple procedure Saponification at 100 C Methylation of free fatty acids at 80 C Methylation for esterified free and fatty acids with HCl catalyst at 100 C React at room temp. Fast and simple procedure GC analysis Reaction time 1.5 hrs Less than 1 min Reaction temperature Quantitative analysis* 37 o C Room temp. Very good Experimental Very good precision * This difference is due to a small amount of free fatty acids in cooking oil. Good Good 26
27 Kit Contents Fatty Acid Methylation Kit (100 tests) Product Name PKG Size QTY Methylation Reagent A 50 ml 1 Methylation Reagent B 50 ml 1 Methylation Reagent C 50 ml 1 Isolation Reagent 250 m 1 Fatty Acid Methyl Ester Purification Kit (50 tests) Product Name PKG Size QTY Conditioning Solution 200 ml 1 Washing Solution 200 ml 1 Eluting Solution 200 ml 1 SPE Cartridge Column - 50 pcs Fatty Acid Methylation Kit for glycerides (100 tests) Product Name PKG Size QTY Solution A (Solvent) 100 ml 1 Solution B (Reaction Solution) 10 ml 1 Solution C (Stop Solution) 100 ml 1 Ordering Information Product Name Grade Storage Product No. PKG Size Fatty Acid Methylation Kit (100 tests) SP RT tests Fatty Acid Methyl Ester Purification Kit (50 tests) SP RT tests Fatty Acid Methylation Kit for glycerides (100 tests) SP RT tests These products are covered by a patent acquired by Gekkeikan Sake Co., Ltd. in collaboration with Dr. Ichihara of Kyoto Integrated Science & Technology Bio-Analysis Center, ASTEM. Nacalai Tesque manufactures and sells these products under a license agreement. [Patent no (Japan)] 27
28 For research use only, not intended for diagnostic or drug use. NACALAI TESQUE, INC. Nijo Karasuma, Nakagyo-ku, Kyoto JAPAN TEL : +81-(0) FAX : +81-(0) Website : info.intl@nacalai.com 1507
Fatty Acid Methylation Kits
Methyl esterification kit for fatty acids analysis Fatty Acid Methylation Kits Below are two methods for efficiently preparing fatty acid samples for GC analysis. Neither method requires high temperatures,
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