Preparation. Sample. Sample Preparation Applications Drugs SUPELCO. Efficiency of Recovery. Efficiency of Recovery

Transcription

1 Applications Drugs ortriptyline CHCH CH HCH Doxepin CHCHCH CH CH *IS = Trimipramine (Internal standard) Imipramine Antidepressants (Tricyclic) From Serum Using Zymark RapidTrace SPE Workstation (SPE/GC) SPE Tube: Discovery DSC-, mg/ml Cat. o.: -U HPLC Column: Discovery C, cm x.mm, µm particles, preceded by a cm C guard column and.µm frit filter Cat. o.: Mobile Phase: MeC:MeH:mM KH P (ph with triethylamine) (::) Flow Rate: ml/min Temp.: ambient Det.: UV, nm Inj.: µl diluted porcine serum extract CH CH CH CH CH Efficiency of Recovery Amitriptyline CHCH CH (CH ) G-, G Concentration Compound (µg/ml) %Recovery %RSD (n=). ortriptyline.. ±... ±.. Doxepin.. ±... ±.. Imipramine.. ±... ±.. Amitriptyline.. ±... ±. SPE Procedure, Using Zymark RapidTrace SPE Workstation Solvent/ Volume Flow Rate Step Solution (ml) (ml/min) Comments. Condition MeH.. conditions sorbent. Condition H.. conditions sorbent. Load spiked porcine serum. A. applies serum sample. Rinse % MeH in H.. washes sorbent. Purge- Cannula H.. cleans sample cannula. Rinse vent.. positions SPE tube over waste port. Dry Time = min dries sorbent. Purge- Cannula MeH.. cleans sample cannula. Collect MeH.. elutes analytes into collection vessel. Collect vent.. pushes residual eluent into vessel B. Purge- Cannula H.. cleans sample cannula A ml porcine serum spiked with.µg/ml or.µg/ml each analyte basified with µl KH, then diluted with ml water B µl water added per ml methanolic eluent before analysis. IS* G Efficiency of Recovery Concentration Compound (µg/ml) %Recovery %RSD (n=). Phenobarbital.. ±... ±.. Aprobarbital.. ±... ±.. Butabarbital.. ±... ±.. Mephobarbital.. ±... ±.. Pentobarbital.. ±... ±.. Secobarbital.. ±... ±. SPE Method For RapidTrace SPE Workstation Application. Condition & equilibrate each tube/well with ml MeH & ml DI Water. Load sample. Wash each tube/well with ml % MeH. Vacuum or air dry with for - min This removes any excess water from the sorbent. The presence of water in the final eluent may prolong eluent evaporation.. Elute with -ml MeH. Dry eluate with nitrogen purge (ºC; - min). Reconstitute with µl mobile phase. Quantify against internal or external standards via HPLC analyses *IS Time (min) Barbiturates from serum using mg/ml Discovery DSC- Lt SPE tubes and Zymark s RapidTrace SPE Workstation. *IS = Barbital (internal standard). Info:.mL porcine serum spike with.µg/ml or.µg/ml each analyte then diluted with.ml water. Barbituates From Serum Using Zymark RapidTrace SPE Workstation (SPE/HPLC) SPE Tube: Discovery DSC-Lt, mg/ml Cat. o.: -U HPLC Column: Discovery C, cm x.mm, µm particles, preceded by a cm C guard column and.µm frit filter Cat. o.: Mobile Phase: MeH/H (:) Flow Rate: ml/min Temp.: C Det.: UV, nm Inj.: µl diluted porcine serum extract G rder:... Technical Service:... Web:

2 Applications Drugs rder:... Technical Service:... Web: Theobromine CH H CH Theophylline CH CH H Paraxanthine CH Bronchodilator: Theophylline and ther Caffeine Metabolites from Serum (SPE/HPLC) SPE Tube: Discovery DSC-, mg/ml Cat. o.: -U Condition: ml methanol, then ml water Apply : ml porcine serum spiked with.µg/ml,.µg/ml, or.µg/ml each analyte Wash and Dry: ml % methanol in water; dry tube min with nitrogen stream Elute: ml methanol; evaporate to dryness with nitrogen stream at room temperature; reconstitute in µl mobile phase containing.µg/ml or -methylxanthine (IS) Column: Discovery RP-AmideC, cm x.mm, µm particles, preceded by a cm RP-AmideC guard column and.µm frit filter Cat. o.: Mobile Phase: methanol:% acetic acid (:) Flow Rate: ml/min Temp.: C Det.: UV, nm Inj.: µl reconstituted porcine serum extract H Efficiency of Recovery CH CH Caffeine CH CH Concentration Compound (µg/ml) %Recovery %RSD (n=). Theobromine.. ±... ±... ±.. Paraxanthine.. ±... ±... ±.. Theophylline.. ±... ±... ±.. Caffeine.. ±... ±... ±. IS* *IS = -Methylxanthine (internal standard) Spiked Serum (.µg/ml) G-, G Serum Blank G, G Efficiency of Recovery Discovery Leading Competitor Compound (µg/ml) DSC-SCX (n=) SCX (n=) % Recovery %RSD %Recovery %RSD. -methylpyrazole (.). ±.%. ±%. -methylpyrazole (.). ±.%. ±% SPE Procedure. Condition & equilibrate with ml MeH & ml DI water.. Load ml urine sample spiked with µg/ml of each analyte.. Wash with ml DI water.. Elute with ml % MeH in mm phosphate buffer, ph... Quantify against external standards via HPLC analyses. Time (min) Competitor Comparison of -methylpyrazole and -methylpyrazole From Urine Using Discovery DSC-SCX (SPE/HPLC) SPE Tube: Discovery DSC-SCX, mg/ml Cat. o.: -U HPLC Column: Discovery C, cm x.mm, µm particle, preceded by a cm guard column and.µm frit filter Cat. o.: Mobile Phase: MeH:mM phosphate buffer, ph (:) Flow Rate: ml/min Temp.: C Det.: UV, nm Inj.: µl diluted urine extract G

3 Applications Mycotoxins, Herbicides. Aflatoxin G. Aflatoxin G. Aflatoxin B. Aflatoxin B. Diquat. Paraquat Aflatoxins (SPE/HPLC) : cornmeal spiked with aflatoxins (ppb G and B, ppb G and B ) Blend g sample for min in ml methanol:water (:) and filter. Extraction Tube: Supelclean LC-C, mg/ml Cat. o.: Conditioning: ml.% aqueous acetic acid Addition: ml filtered extract + ml.% aqueous acetic acid Washing: µl % THF in.% aqueous acetic acid, then ml hexane. Dry packing under nitrogen. ml % THF in hexane. Dry packing min under nitrogen. Elution: x ml % THF in methylene chloride. Evaporate eluate to dryness under nitrogen. Reconstitute with µl of methanol, then dilute with µl of.% aqueous acetic acid. Column: SIL LC-, cm x.mm ID, µm particles (with guard column) Cat. o.: Mobile Phase: methanol:acetonitrile:water (.:.:) Flow Rate:.mL/min Det.: VIS, nm Inj.: µl - Paraquat and Diquat (SPE/HPLC) Refer to US EPA Method. for full details. : ml drinking water, adjust sample ph to. ±. with sodium hydroxide solution (% w/v) or hydrochloric acid solution (% v/v) Extraction Disk: EVI- DSK, mm Cat. o.: Conditioning: ml methanol x ml reagent water ml conditioning solvent A (.g cetyl trimethyl ammonium bromide and ml conc. ammonium hydroxide in ml water, dilute to L) x ml reagent water ml conditioning solvent B (.g hexanesulfonic acid, sodium salt and ml ammonium hydroxide in ml deionized water, dilute to ml) Addition: adjust vacuum to flow rate of ml/min Extraction:. to.ml methanol (to cover disk) x ml eluting solution (.ml orthophosphoric acid and.ml diethylamine in ml water, dilute to L) Column: SIL LC-, cm x.mm ID, µm particles Cat. o.: -U Mobile Phase:.mL triethylamine and.g -hexane-sulfonic acid, sodium salt to ml deionized water add orthophosphoric acid to ph =., dilute to L Flow Rate:.mL/min Temp.: C Det.: Photodiode array, quantify Diquat -nm, Paraquat -nm Inj.: µl - rder:... Technical Service:... Web:

4 Applications Herbicides. Tetrachloroterephthalic acid. Picloram. Dicamba.,-D.,,-T.,,-TP. Dinoseb. Dacthal. Pentachlorophenol Gradient Program Time (min) % B.... Analyte Recovery (± SD, n = ). Simazine. ±.. Atrazine. ±.. Propazine. ±. rder:... Technical Service:... Web: Extracted Background Figures provided by A. Lichtman, assau County Dept. of Health, Hempstead, ew York, USA. Request Application ote o. T. Acidic Herbicides in Water (SPE/HPLC) -, - Extraction Tube: EVI-Carb, mg/ml Cat. o.: Column: polymeric-coated silica-based PAH specialty column, cm x mm ID, µm particles Supelco Equivalent: SIL LC-PAH(available on request) Mobile Phase: gradient, A = water/.% H P, B = acetonitrile Temp.: C Flow Rate:.mL/min Det.: photodiode array: peak width. min sampling interval. sec, monitor nm & nm Inj.: µl of extract (-ppb each analyte in water) Triazine Herbicides from Grass (SPE/HPLC) : g fresh grass clippings spiked with ppm each herbicide. Add g anhydrous sodium sulfate and ml methylene chloride:acetone (:) Shake min and allow mixture to stand min Extraction Tube: Supelclean LC-SCX, mg/ml Cat. o.: Conditioning: ml methylene chloride Addition: ml grass extract Washing: x ml acetonitrile Dry packing for min under nitrogen x ml deionized water Elution:.mL methanol Dilute to ml with deionized water. Column: SIL LC--DB, cm x.mm ID, µm particles * Cat. o.: Mobile Phase: acetonitrile:water (:) Flow Rate:.mL/min Det.: UV, nm Inj.: µl * With Supelguard LC--DB column,.cm x.mm ID, µm particles -

5 Applications PCBs, Pesticides PCB (SPE/GC) : used transformer oil containing ppb Aroclor Extraction Tube: Supelclean LC-Florisil,.g/mL Cat. o.: Extraction Tube: Supelclean LC-Si,.g/mL Cat. o.: Connect tubes in series, LC-Florisil on top Conditioning: µl isooctane Addition:.g, weigh onto top of frit of tube Drying: none Elution: x ml isooctane, up to ml in volumetric flask Column: SPB-, m x.mm ID,.µm film Cat. o.: ven: C ( min) to C at C/min, hold min Carrier: nitrogen Det.: ECD Inj.: µl splitless ( sec delay), then split (:) Blank Extract For extraction recovery data, request Bulletin T. Chromatograms provided by J. Fillion, Agriculture and Agri-Food Canada (Laboratory Services Division, Pesticide Laboratory), ttawa, ntario. Diphenylamine Pesticide-Containing (relative abundance scale X blank) Methoxychlor Pesticides in Fruits and Vegetables (SPE/GC) : homogenize g sample in ml acetonitrile then add g acl and homogenize min Extraction Tube: EVI-Carb, mg/ml Cat. o.: Extraction: Centrifuge ml of acetonitrile layer at high speed for min. Evaporate ml aliquot to.ml under nitrogen at C. Transfer sample to SPE tube. Elute pesticides with ml acetonitrile:toluene (:). Concentrate to ml by rotary evaporation. Add x ml acetone, co ncentrating material to ml after each addition. Add µl cis-chlordane in acetone (ng/µl) then dilute to.ml with acetone. Supelco Equivalent: SPB- (available on request) Column: % cyanopropylphenyl/% dimethylsiloxane, m x.mm ID,.µm film ven: C (hold min) to C at C; C to C at C/min; C to C at C/min and hold. min Carrier: helium Det.: MSD, C Inj.: µl, splitless - -, - rder:... Technical Service:... Web:

6 Applications Pesticides rder:... Technical Service:... Web: Analyte % Recovery (± RSD, n = ). xamyl ±.. Methomyl ±.. --aphthylthiourea. Aldicarb ±.. Simazine ±.. Monuron ±.. Cyanazine ±.. Metribuzin ±.. Carbofuran ±.. Atrazine ±.. Carbaryl ±.. Diuron ±.. Propham ±.. Propachlor ±.. Linuron ±. Standard onvolatile Pesticides (SPE/HPLC) : water spiked with pesticides (-µg/l each component) Extraction Tube: Supelclean EVI-Carb, mg/ml Cat. o.: Conditioning: ml methylene chloride:methanol (:) ml methanol ml % acetic acid in water Keep bed moist until sample is added. Addition: ml, ml/min Drying: min with vacuum suction Elution:.mL methanol x.ml methylene chloride:methanol (:) Dry eluate to µl under gentle stream of nitrogen Reconstitute to ml with methanol. Column: SIL LC--DB, cm x.mm ID, µm particles Cat. o.: -U Mobile Phase: A = water:acetonitrile (:), B = acetonitrile % A for min then to % A over min Flow Rate:.mL/min Det.: UV, nm Inj.: µl Blank - Analyte % Recovery (± CV). Hexachlorobenzene ±. γ-bhc (Lindane) ±. Heptachlor ±. Aldrin ±. Heptachlor epoxide ±. Endrin ±. Methoxychlor ± Extracted Pesticides Blank Chlorinated Pesticides (SPE/GC) : drinking water spiked with pesticides Extraction Tube: Supelclean EVI-, mg/ml Cat. o.: Conditioning: x ml hexane:ethyl ether (:) ml methanol ml deionized water Addition: ml, ml/min Drying: min Elution: x.ml hexane:ethyl ether (:) Concentrate eluate to ml under stream of nitrogen. Column: PTE-, m x.mm ID,.µm film Cat. o.: -U ven: C ( min) to C at C/min Carrier: helium Det.: ECD, C Inj.: µl -,

7 Applications Pesticides, Phenols. α-bhc. β-bhc. γ-bhc (Lindane). δ-bhc. Heptachlor. Aldrin. Heptachlor epoxide. γ-chlordane. Endosulfan I. α-chlordane. Dieldrin Standard.,'-DDE. Endrin. Endosulfan II.,'-DDD. Endrin aldehyde. Endosulfan sulfate.,'-ddt. Endrin ketone. Methoxychlor. Decachlorobiphenyl (Surr.) Extracted Surr. Chlorinated Pesticides in Hazardous Waste (SPE/GC) : ml aqueous hazardous waste, adjust ph to - if necessary, add ml methanol Extraction Tube: EVI-, glass, mg/ml Cat. o.: (.g) or (g) Conditioning: ml methanol, (do not allow bed to dry out) ml % methanol in water Addition: adjust vacuum to flow rate of ml/min Dry: purge tube with nitrogen for - min (use Visidry attachment) Extraction: x ml hexane:acetone (:), allow to soak, elute dropwise Column: PTE- QTM, m x.mm ID,.µm film Cat. o.: ven: C (.min) to C (min) Carrier: helium Det.: ECD, C Inj.: µl, C, split/splitless ( sec delay) Surr. -, Analyte % Recovery (± CV). Phenol. ±.. -Chlorophenol. ±.. -Methylphenol. ±.. -Bromophenol (Int. Std.). -Methylphenol. ±.. -itrophenol. ±..,-Dimethylphenol. ±..,-Dichlorophenol. ±.. -Chloro--methylphenol. ±..,,-Trichlorophenol. ±.. -itrophenol. ±..,,,-Tetrachlorophenol. ±.. Pentachlorophenol. ±. Phenols (SPE/GC) : water spiked with phenols Extraction Tube: Supelclean EVI-Chrom P, mg/ml Cat. o.: -U Conditioning: ml methyl t-butyl ether* ml methanol ml deionized water Addition: ml Drying: min, vacuum suction Elution: ml methyl t-butyl ether,* allow to soak with vacuum off ml methyl t-butyl ether,* elute dropwise Additional methyl t-butyl ether until ml of eluate is collected. Column: PTE- QTM, m x.mm ID,.µm film Cat. o.: ven: C to C at C/min, hold min, then to C at C/min, hold min Carrier: helium Det.: FID, C Inj.: µl splitless ( sec hold) * Can substitute ethyl acetate. - rder:... Technical Service:... Web:

8 Applications Polynuclear Aromatic Hydrocarbons, Semivolatiles rder:... Technical Service:... Web: PAHs in Water (SPE/GC) : water spiked with PAHs Extraction Tube: Supelclean EVI-, mg/ml Cat. o.: Conditioning: x ml toluene:methanol (:), ml methanol, ml deionized water Addition: ml, ml/min Drying: min Elution: x ml toluene:methanol (:) Column: PTE-, m x.mm ID,.µm film Cat. o.: -U ven: C ( min) to C at C/min Carrier: helium Det.: FID, C Inj.: µl : :, :,, µg/l*. Acenapthene -d.. Phenanthrene -d.. Chrysene -d.. Hexachlorocyclopentadiene.. Dimethylphthalate.. Acenaphthylene.. -Chlorobiphenyl.. Diethyphthalate.. Fluorene..,-Dichlorobiphenyl.. Hexachlorobenzene.. Simazine.. Atrazine.. Pentachlorophenol.. γ-bhc.. Phenanthrene.. Anthracene.,.,,-Trichlorobiphenyl.,,. Alachlor.. Heptachlor.. di-n-butylphthalate..,',,'-tetrachlorobiphenyl.. Aldrin. Semivolatiles (SPE/GC) : L drinking water, ph to < with HCl, add ml methanol and mix thoroughly Extraction Disk: EVI- DSK, mm Cat. o.: Conditioning: ml dichloromethane (pull completely through disk)/ml methanol (do not allow disk to dry out)/ml reagent water Addition: adjust vacuum to flow rate of ml/min Extraction: rinse sample container with x ml acetonitrile, extract disk with solution Column: % diphenyl/% dimethyl silicone capillary, m x.mm ID,.µm film ven: C to C ( min), then to C ( min) at C/min Supelco Equivalent: Equity- (Cat. o. -U) Carrier: helium, cm/sec Det.: MS, scan range m/z=- Inj.: µl, split/splitless, min delay : Analyte %Recovery(± CV). Acenaphthylene ±. Fluorene ±.. Phenanthrene ±. Anthracene ±. Pyrene ±.. Benzo(a)anthracene ±.. Chrysene ±.. Benzo(b)fluoranthene ±. Benzo(k)fluoranthene ±.. Indeno(,,-cd)pyrene ±. Benzo(a)pyrene ±. Dibenzo(a,h)anthracene ±.. Benzo(ghi)perylene ± - µg/l*. Heptachlor epoxide..,',',,-pentachlorobiphenyl.. γ-chlordane.. Pyrene.. α-chlordane.. trans-onachlor..,',,',,'-hexachlorobiphenyl.. Endrin.. Butylbenzylphthalate.. di(-ethylhexyl)adipate..,',',,',-heptachlorobiphenyl.. Methoxychlor..,',',,',,'-ctachlorobiphenyl.. Benz(a)anthracene.. Chrysene.. Di(-ethylhexyl)phthalate.. Benzo(b)fluoranthene.. Benzo(k)fluoranthene.. Benzo(a)pyrene.. Perylene -d.. Indeno(,, -cd)pyrene.. Dibenz(a,h)anthracene.. Benzo(ghi)perylene. -

9 Applications Volatiles. Dichlorodifluoromethane. Chloromethane. Vinyl chloride. Bromomethane. Chloroethane. Trichlorofluoromethane.,-Dichloroethylene. Methylene chloride. trans-,-dichloroethylene.,-dichloroethane.,-dichloropropane. cis-,-dichloroethylene. Chloroform. Bromochloromethane.,,-Trichloroethane PID ELCD.,-Dichloropropane. Carbon tetrachloride.,-dichloroethane. Benzene. Trichloroethylene.,-Dichloropropane. Bromodichloromethane. Dibromomethane. cis-,-dichloropropene. Toluene. trans-,-dichloropropene.,,-trichloroethane.,-dichloropropane. Tetrachloroethylene. Chlorodibromomethane Volatile Compounds by US EPA Method. (PT/GC) : ml water ( ppb each analyte) Column: VCL, m x.mm ID,.µm film Instrument: I Analytical Model Cat. o.: Trap: VCARB ven: C ( min) to C at C/min, hold Cat. o.: -U Carrier: helium,.ml/min Purge: min, at C, ml/min Det.: ELCD (I Model )/PID(I Model ) in series Desorption:.min at C Chromatograms provided courtesy of I Analytical, College Station, TX..,-Dibromoethane. Chlorobenzene.,,,-Tetrachloroethane. Ethylbenzene. m-xylene. p-xylene. o-xylene. Styrene. Isopropylbenzene. Bromoform.,,,-Tetrachloroethane.,,-Trichloropropane. n-propylbenzene. Bromobenzene.,,-Trimethylbenzene,,,. -Chlorotoluene. -Chlorotoluene. tert-butylbenzene.,,-trimethylbenzene. sec-butylbenzene. p-isopropyltoluene.,-dichlorobenzene.,-dichlorobenzene. n-butylbenzene.,-dichlorobenzene.,-dibromo--chloropropane.,,-trichlorobenzene. Hexachlorobutadiene. aphthalene.,,-trichlorobenzene -, rder:... Technical Service:... Web:

10 Applications Volatiles rder:... Technical Service:... Web: Dichlorodifluoromethane. Chloromethane. Vinyl chloride. Bromomethane. Chloroethane. Trichlorofluoromethane.,-Dichloroethylene. Methylene chloride. trans-,-dichloroethylene.,,-dichloroethane.,-dichloropropane. cis-,-dichloroethylene. Chloroform. Bromochloromethane.,,-Trichloroethane.,-Dichloropropene. Carbon tetrachloride.,-dichloroethane. Benzene. Fluorobenzene (int std). Trichloroethylene.,-Dichloropropane. Bromodichloromethane. Dibromomethane. cis-,-dichloropropene. Toluene. trans-,-dichloropropene.,,-trichloroethane.,-dichloropropane. Tetrachloroethylene. Chlorodibromomethane.,-Dibromoethane o Cryogenics Volatile Compounds by US EPA Method. (PT/GC) : ppb each component in ml water Trap: VCARB Cat. o.: -U Purge: min, ml/min Dry: min Desorb. Temp: C for min Bake: C for min C. Chloromethane. Vinyl chloride. Bromomethane. Chloroethane. Trichlorofluoromethane.,-Dichloroethylene. Methylene chloride o Cryogenics,. Chlorobenzene.,,,-Tetrachloroethane. Ethylbenzene. m-xylene. p-xylene. o-xylene. Styrene. Isopropylbenzene. Bromoform.,,,-Tetrachloroethane.,,-Trichloropropane. n-propylbenzene. Bromobenzene.,,-Trimethylbenzene. -Chlorotoluene. -Chlorotoluene,, Column: VCL, m x.mm ID,.µm film Cat. o.: ven: C ( min) to C at C/min, hold min Carrier: helium, ml/min Det.: MS, Scan Range m/z = - at. sec/scan Volatile Compounds by US EPA Method (PT/GC) : ppb each component in ml water Bake: C for min Trap: VCARB Column: VCL, m x.mm ID,.µm film Cat. o.: -U Cat. o.: Purge: min, ml/min ven: C ( min) to C at C/min Dry Purge: min Carrier: helium,.ml/min Desorb. Temp: C for min Det.: MS, Scan Range m/z = - at. sec/scan. trans-,-dichloroethylene.,,-dichloroethane. Chloroform. Bromochloromethane (IS).,,-Trichloroethane. Carbon tetrachloride. Benzene,,,.,-Dichloroethane. Difluorobenzene (IS). Trichloroethylene.,-Dichloropropane. Bromodichloromethane. -Chloroethyl vinyl ether. cis-,-dichloropropene. tert-butylbenzene.,,-trimethylbenzene. sec-butylbenzene. p-isopropyltoluene.,-dichlorobenzene.,-dichlorobenzene. n-butylbenzene.,-dichlorobenzene-d (int std).,-dichlorobenzene.,-dibromo--chloropropane.,,-trichlorobenzene. Hexachlorobutadiene. aphthalene.,,-trichlorobenzene -. Toluene. trans-,-dichloropropene.,,-trichloroethane. Tetrachloroethylene. Chlorodibromomethane. Chlorobenzene-d (IS). Chlorobenzene. Ethylbenzene. Bromoform.,,,-Tetrachloroethane.,-Dichlorobenzene.,-Dichlorobenzene.,-Dichlorobenzene -

11 Applications Volatiles, Volatile Compounds by US EPA Method (PT/GC) : ppb each component in ml water Column: VCL, m x.mm ID,.µm film Trap: VCARB Cat. o.: Cat. o.: -U ven: C ( min) to C at C/min Purge: min, ml/min Carrier: helium,.ml/min Dry Purge: min Det.: MS, Scan Range m/z = - at. sec/scan Desorb.: C for min Bake: C for min C o Cryogenics Volatile Compounds by US EPA Method (PT/GC) : ppb each componentin ml water Column: VCL, m x.mm ID,.µm film Trap: VCARB Cat. o.: -U Cat. o.: -U ven: C ( min) to Cat C/min Purge: min, ml/min Carrier: helium,.ml/min Dry Purge: min Det.: MS, Scan Range m/z = - at. sec/scan Desorb.: C for min Bake: C for min. Chloromethane. Vinyl chloride. Bromomethane. Chloroethane. Trichlorofluoromethane.,-Dichloroethene. Methylene chloride. trans-,-dichloroethene.,-dichloroethane. Chloroform.,,-Trichloroethane. Carbon tetrachloride. Chloromethane. Vinyl chloride. Bromomethane. Chloroethane. Trichlorofluoromethane.,-Dichloroethylene. Methylene chloride. trans-,-dichloroethene.,,-dichloroethane. Chloroform.,,-Trichloroethane. Benzene.,-Dichloroethane. Trichloroethene.,-Dichloropropane. Bromodichloromethane. -Chloroethylvinyl ether. cis-,-dichloropropene. Toluene. trans-,-dichloropropene.,,-trichloroethane. Tetrachloroethene. Dibromochloromethane. Chlorobenzene. Ethyl benzene. Bromoform.,,,-Tetrachloroethane.,-Dichlorobenzene.,-Dichlorobenzene.,-Dichlorobenzene. Carbon tetrachloride. Benzene.,-Dichloroethane. Trichloroethylene.,-Dichloropropane. Bromodichloromethane. -Chloroethyl vinyl ether. cis-,-dichloropropene. Toluene. trans-,-dichloropropene.,,-trichloroethane. Tetrachloroethylene. Dibromochloromethane. Chlorobenzene. Ethylbenzene. Bromoform.,,,-Tetrachloroethane.,-Dichlorobenzene.,-Dichlorobenzene.,-Dichlorobenzene - - rder:... Technical Service:... Web: