Mass Spectrometry. - Introduction - Ion sources & sample introduction - Mass analyzers - Basics of biomolecule MS - Applications
|
|
- Virgil Douglas
- 5 years ago
- Views:
Transcription
1 - Introduction - Ion sources & sample introduction - Mass analyzers - Basics of biomolecule MS - Applications Adapted from Mass Spectrometry in Biotechnology Gary Siuzdak,, Academic Press
2 Introduction Mass Spectrometry - In the past, mass spectrometry was confined to the realm of small l molecules; large molecules did not survive the desorption and ionization process intact. - Matrix-assisted assisted laser desorption/ionization (MALDI). - Electrospray ionization (ESI) - Fast atom/ion bombardment (FAB) - The analysis of proteins, peptides, carbohydrates, oligonucleotides, natural products and drug metabolites. - Detection capabilities ranging from the picomole to the femtomole e level. - Molecular weight accuracy on the order of 0.01%. - The utilities of MS include molecular weight characterization, noncovalent n interactions, protein and peptide sequencing, DNA sequencing, protein folding, in vitro drug analysis, and drug discovery. 2
3 Introduction Mass Spectrometry m / z Light source Prism Light detector 3
4 - Matrix-assisted assisted laser desorption/ionization - A nonvolatile solid as the matrix. 4
5 - Matrix-assisted assisted laser desorption/ionization 5
6 - Electrospray ionization Mass Spectrometry 6
7 - Electrospray ionization Mass Spectrometry 7
8 - Electrospray ionization myoglobin Mass Spectrometry z 1 x 1542 = M + z 1 (z 1-1) x 1696 = M + (z 1-1) z 1 z 1-1 z 1 = 11 M = 16,951 8
9 Mass analyzers Mass Spectrometry - Quadrupole - Time-of of-flight flight & time-of of-flight flight reflectron - Ion trap - Magnetic - Double-focusing magnetic sector - FT-IR (ion cyclotron 9
10 Mass analyzers Mass Spectrometry Resolution is the ability of a mass spectrometer to distinguish between ions of different mass-to to-charge ratios. Therefore, greater resolution corresponds directly to the increased ability to differentiate ions. Resolution = M / M M = M 1 / (M 1 M 2 ) M M = full width at half maximum (FWHM) 10
11 - Quadruple analyzer Mass Spectrometry 11
12 - Time-of flight analyzer & time-of of-flight flight reflectron 12
13 - Tandem mass spectrometry 13
14 - Tandem mass spectrometry 離子源 質譜儀 -I 解離室 質譜儀 -II 質譜圖 子離子掃瞄 Daughter Scan ABC DEF mass ABC only AB, BC ABC scan all masses AB BC ABC 母離子掃瞄 Parent Scan ABC DEC scan all masses AB, DE C, etc. mass C only DEC ABC 中性丟失掃瞄 Parent Scan ABC DBF scan all masses AC, DF AB, etc. mass minus B DF AC 選擇反應偵測 SRM ABC DEF mass ABC only AB, BC ABC* mass BC only BC 14
15 15
16 Basics of biomolecules mass spectrometry - 1. Salt content should be minimized. 2. Matrix selection/preparation. 3. Care should be taken to maintain high purity. 4. Sample solubility in the solvent or matrix is crucial. 5. Is there enough amount of sample? 6. The functional groups help to determine how to analyze. 7. Analyze soon after synthesis and/or purification. 16
17 Basics of biomolecules mass spectrometry Solubility: 14-kDa protein electrospray mass analyzed methanol 33% methanol 33% acetonitrile 33% water 17
18 Basics of biomolecules mass spectrometry MALDI matrix 18
19 Applications Mass Spectrometry 19
20 Applications Mass Spectrometry Protein folding 20
21 Overview of protein identification } TagIdent - Primary attributes & Secondary attributes. - Most attributes relate directly or indirectly to a protein s s sequence, however they vary in the way that they are generated and the protein property they represent. - Crossed references. Adapted from Micromass and Proteome Research: New Frontiers in Functional Genomics 21
22 ESI- Tandem MS MALDI-TOF MS 22
23 Sample Preparation for Mass Spectrometric Analysis - Pure proteins or protein spots from 1-D 1 D or 2-D 2 D gel electrophoresis - Protein digestion with proteases to yield fragments that are most t compatible with MS analysis, 6-20 amino acids (i.e. Trypsin: a 50kDa to 30 tryptic peptides). - Non-specific protease and CNBr. - Extraction of peptides from gel spots. - Exact masses of the resulting peptides. - Fragmentation of peptide ions in MS analysis fmol level for peptide mapping fmol level for peptide sequencing. 23
24 Peptide mass fingerprinting (PMF) or mapping Trypsin * * * * * * * 24
25 Peptide mass fingerprinting (PMF) or mapping 25
26 Peptide mass fingerprinting (PMF) or mapping using MASCOT 26
27 Peptide fragmentation to generate partial sequence Sequencing Fragmentation 27
28 Selection of charged peptides 28
29 Transformation & de-isotoping of raw MS-MS data. 29
30 Sequencing results from MS-MS of m/z (M+3H) 3+ 30
31 Sequence reading L = I, Q/K = G+A, W = G+E = A+D 31
32 Sequencing results from MS-MS of m/z (M+3H) 3+ β-galactosidase Q (89%) versus G (9%) A (4%) at position 3 W (35%) versus G (65%) E (65%) at position 18 G (80%) E (80%) versus A (19%) D (19%) at position 21 32
33 Peptide sequencing using MASCOT 33
34 Peptide sequencing using MASCOT 34
35 Protein identification by peptide sequencing 35
36 Peptide sequencing using chemical modifications lysine to homoarginine Partial sequencing plus MALDI-TOF 36
37 Amino acid residue modifications 37
38 Chemical modifications 38
39 Post-translational translational modifications 39
40 Quantitative analysis using isotope-coded affinity tags (ICAT) Nature biotechnology (1999) 17:
Mass Spectrometry. Mass spectrometer MALDI-TOF ESI/MS/MS. Basic components. Ionization source Mass analyzer Detector
Mass Spectrometry MALDI-TOF ESI/MS/MS Mass spectrometer Basic components Ionization source Mass analyzer Detector 1 Principles of Mass Spectrometry Proteins are separated by mass to charge ratio (limit
More information2. Ionization Sources 3. Mass Analyzers 4. Tandem Mass Spectrometry
Dr. Sanjeeva Srivastava 1. Fundamental of Mass Spectrometry Role of MS and basic concepts 2. Ionization Sources 3. Mass Analyzers 4. Tandem Mass Spectrometry 2 1 MS basic concepts Mass spectrometry - technique
More informationBiological Mass Spectrometry. April 30, 2014
Biological Mass Spectrometry April 30, 2014 Mass Spectrometry Has become the method of choice for precise protein and nucleic acid mass determination in a very wide mass range peptide and nucleotide sequencing
More informationComparison of mass spectrometers performances
Comparison of mass spectrometers performances Instrument Mass Mass Sensitivity resolution accuracy Quadrupole 1 x 10 3 0.1 Da* 0.5-1.0 pmol DE-MALDI 2 x 10 4 20 ppm 1-10 fmol peptide 1-5 pmol protein Ion
More informationMass Spectrometry Course Árpád Somogyi Chemistry and Biochemistry MassSpectrometry Facility) University of Debrecen, April 12-23, 2010
Mass Spectrometry Course Árpád Somogyi Chemistry and Biochemistry MassSpectrometry Facility) University of Debrecen, April 12-23, 2010 Introduction, Ionization Methods Mass Analyzers, Ion Activation Methods
More informationMALDI-TOF. Introduction. Schematic and Theory of MALDI
MALDI-TOF Proteins and peptides have been characterized by high pressure liquid chromatography (HPLC) or SDS PAGE by generating peptide maps. These peptide maps have been used as fingerprints of protein
More informationMethods in Mass Spectrometry. Dr. Noam Tal Laboratory of Mass Spectrometry School of Chemistry, Tel Aviv University
Methods in Mass Spectrometry Dr. Noam Tal Laboratory of Mass Spectrometry School of Chemistry, Tel Aviv University Sample Engineering Chemistry Biology Life Science Medicine Industry IDF / Police Sample
More informationIon Source. Mass Analyzer. Detector. intensity. mass/charge
Proteomics Informatics Overview of spectrometry (Week 2) Ion Source Analyzer Detector Peptide Fragmentation Ion Source Analyzer 1 Fragmentation Analyzer 2 Detector b y Liquid Chromatography (LC)-MS/MS
More informationBiological Mass spectrometry in Protein Chemistry
Biological Mass spectrometry in Protein Chemistry Tuula Nyman Institute of Biotechnology tuula.nyman@helsinki.fi MASS SPECTROMETRY is an analytical technique that identifies the chemical composition of
More information1. Sample Introduction to MS Systems:
MS Overview: 9.10.08 1. Sample Introduction to MS Systems:...2 1.1. Chromatography Interfaces:...3 1.2. Electron impact: Used mainly in Protein MS hard ionization source...4 1.3. Electrospray Ioniztion:
More informationLecture 3. Tandem MS & Protein Sequencing
Lecture 3 Tandem MS & Protein Sequencing Nancy Allbritton, M.D., Ph.D. Department of Physiology & Biophysics 824-9137 (office) nlallbri@uci.edu Office- Rm D349 Medical Science D Bldg. Tandem MS Steps:
More informationFundamentals of Soft Ionization and MS Instrumentation
Fundamentals of Soft Ionization and MS Instrumentation Ana Varela Coelho varela@itqb.unl.pt Mass Spectrometry Lab Analytical Services Unit Index Mass spectrometers and its components Ionization methods:
More informationSCS Mass Spectrometry Laboratory
SCS Mass Spectrometry Laboratory Contact Information Staff 31 Noyes Laboratory (8:00-5:00 M-F) 217-333-2545 http://scs.illinois.edu/massspec/ Furong Sun (frs@illinois.edu) Furong Sun Director Training
More informationIntroduction to Proteomics 1.0
Introduction to Proteomics 1.0 CMSP Workshop Pratik Jagtap Managing Director, CMSP Objectives Why are we here? For participants: Learn basics of MS-based proteomics Learn what s necessary for success using
More informationLiquid Chromatography Mass Spectrometer 液相層析質譜儀
Liquid Chromatography Mass Spectrometer 液相層析質譜儀 speaker: 應用化學系黃立心 date: 2015/4/9 LC-MS of 貴重 / 共同儀器中心 2 設備 LC-QqQ MS 液相層析 - 三段四極柱式質譜 LC-Q-TOF MS 液相層析 - 四極柱 - 飛行時間式質譜 游離方式 ESI 電灑游離 APPI 大氣壓力光游離 田家炳光電大樓
More informationSolving practical problems. Maria Kuhtinskaja
Solving practical problems Maria Kuhtinskaja What does a mass spectrometer do? It measures mass better than any other technique. It can give information about chemical structures. What are mass measurements
More informationNew Instruments and Services
New Instruments and Services Liwen Zhang Mass Spectrometry and Proteomics Facility The Ohio State University Summer Workshop 2016 Thermo Orbitrap Fusion http://planetorbitrap.com/orbitrap fusion Thermo
More informationMass spectra of peptides and proteins - and LC analysis of proteomes Stephen Barnes, PhD
Mass spectra of peptides and proteins - and LC analysis of proteomes Stephen Barnes, PhD 4-7117 sbarnes@uab.edu Overview A mass spectrum Electrospray MS Analysis of intact proteins Molecular weight calculations
More informationMass Spectrometry and Proteomics - Lecture 1 - Matthias Trost Newcastle University
Mass Spectrometry and Proteomics - Lecture 1 - Matthias Trost Newcastle University matthias.trost@ncl.ac.uk Content Lectures 1-3 The basics of mass measurement Ionisation techniques Mass analysers Detectors
More informationMass Spectrometry at the Laboratory of Food Chemistry. Edwin Bakx Laboratory of Food Chemistry Wageningen University
Mass Spectrometry at the Wageningen University Mass Spectrometry at the 3 UPLC/CE - ESI - Ion trap MS systems UPLC Thermo Acella with a Velos or VelosPro CE Beckman PA800 with a Thermo VelosPro 1 UPLC-
More informationIonization Methods. Neutral species Charged species. Removal/addition of electron(s) Removal/addition of proton(s)
Ionization Methods Neutral species Charged species Removal/addition of electron(s) M + e - (M +. )* + 2e - electron ionization Removal/addition of proton(s) M + (Matrix)-H MH + + (Matrix) - chemical ionization
More informationPeptide sequencing using chemically assisted fragmentation (CAF) and Ettan MALDI-ToF Pro mass spectrometry
application note Ett MALDI-ToF Pro Peptide sequencing using chemically assisted fragmentation (CAF) d Ett MALDI-ToF Pro mass spectrometry Key words: MALDI-ToF, PSD, peptide sequencing, chemically assisted
More informationLab 1 - Klinisk Kemisk Diagnos9k
Lab 1 - Klinisk Kemisk Diagnos9k Mass Spectrometry Quan%fica%on & Protein iden%fica%on by pep%de mass fingerprint melinda.rezeli@bme.lth.se 073-978 0298 Lab 1 - Klinisk Kemisk Diagnos9k MALDI mass spectrometry
More informationDouble charge of 33kD peak A1 A2 B1 B2 M2+ M/z. ABRF Proteomics Research Group - Qualitative Proteomics Study Identifier Number 14146
Abstract The 2008 ABRF Proteomics Research Group Study offers participants the chance to participate in an anonymous study to identify qualitative differences between two protein preparations. We used
More informationAgilent Protein In-Gel Tryptic Digestion Kit
Agilent 5188-2749 Protein In-Gel Tryptic Digestion Kit Agilent Protein In-Gel Tryptic Digestion Kit Instructions Kit Contents The Protein In-Gel Tryptic Digestion Kit includes sufficient reagents for approximately
More informationProteomics/Peptidomics
Proteomics/Peptidomics System biology tools and preclinical models for translational research in endometriosis, ESHRE Campus workshop, 4-5 September 2009 E. Waelkens Proteomics: What? Proteins Proteomics
More informationPosterREPRINT AN AUTOMATED METHOD TO SELF-CALIBRATE AND REJECT NOISE FROM MALDI PEPTIDE MASS FINGERPRINT SPECTRA
Overview AN AUTOMATED METHOD TO SELF-CALIBRATE AND REJECT NOISE FROM MALDI PEPTIDE MASS FINGERPRINT SPECTRA Jeffery M Brown, Neil Swainston, Dominic O. Gostick, Keith Richardson, Richard Denny, Steven
More informationQuadrupole and Ion Trap Mass Analysers and an introduction to Resolution
Quadrupole and Ion Trap Mass Analysers and an introduction to Resolution A simple definition of a Mass Spectrometer A Mass Spectrometer is an analytical instrument that can separate charged molecules according
More informationMetabolomics: quantifying the phenotype
Metabolomics: quantifying the phenotype Metabolomics Promises Quantitative Phenotyping What can happen GENOME What appears to be happening Bioinformatics TRANSCRIPTOME What makes it happen PROTEOME Systems
More informationIn-Gel Tryptic Digestion Kit
INSTRUCTIONS In-Gel Tryptic Digestion Kit 3747 N. Meridian Road P.O. Box 117 Rockford, IL 61105 89871 1468.2 Number Description 89871 In-Gel Tryptic Digestion Kit, sufficient reagents for approximately
More informationMass Spectrometry and Proteomics. Professor Xudong Yao Bioanalytical Chemistry Spring 2007
Mass Spectrometry and Proteomics Professor Xudong Yao Bioanalytical Chemistry Spring 2007 Proteomics and -omics Roles of mass spectrometry Comparative proteomics Chemical proteomics Protein, Proteome and
More informationPTM Discovery Method for Automated Identification and Sequencing of Phosphopeptides Using the Q TRAP LC/MS/MS System
Application Note LC/MS PTM Discovery Method for Automated Identification and Sequencing of Phosphopeptides Using the Q TRAP LC/MS/MS System Purpose This application note describes an automated workflow
More information(III) MALDI instrumentation
Dr. Sanjeeva Srivastava (I) Basics of MALDI-TF (II) Sample preparation In-gel digestion Zip-tip sample clean-up Matrix and sample plating (III) MALDI instrumentation 2 1 (I) Basics of MALDI-TF Analyte
More informationMALDI Imaging Drug Imaging Detlev Suckau Head of R&D MALDI Bruker Daltonik GmbH. December 19,
MALDI Imaging Drug Imaging Detlev Suckau Head of R&D MALDI Bruker Daltonik GmbH December 19, 2014 1 The principle of MALDI imaging Spatially resolved mass spectra are recorded Each mass signal represents
More informationComponents of a Mass Spectrometer
Components of a Mass Spectrometer Sample Introduction Inlet GC LC Direct Insertion (Syringe/Probe) Ionization Ion Separation Ion Detection Ion Source EI,CI,,, MALDI Mass Analyzer Under vacuum TOF, Quadrupole,
More informationSUNY UPSTATE MEDICAL UNIVERSITY PROTEOMICS CORE
SUNY UPSTATE MEDICAL UNIVERSITY PROTEOMICS CORE Location: SUNY Upstate Medical University Weiskotten Hall Addition, Room 4303 750 East Adams Street Syracuse, NY 13210 Contact Information: David Kakhniashvili,
More informationAn Alternative Approach: Top-Down Bioanalysis of Intact Large Molecules Can this be part of the future? Lecture 8, Page 27
An Alternative Approach: Top-Down Bioanalysis of Intact Large Molecules Can this be part of the future? Lecture 8, Page 27 Top-down HRAM Bioanalysis of Native Proteins/Molecules Relative Abundance 100
More information5/11/2015 MASS SPECTROMETRY. Mass spectrometry (MS) - overview. Mass spectrometer. Ionization and mass analyzer. Analyzer: QUADRUPOLE
UNIVERSITY OF PÉCS MEDICAL SCHOOL www.medschool.pte.hu MASS SPECTROMETRY Mass spectrometry (MS) - overview SAMPLE: charged/ionized sample(fluid biological samples, proteins, peptides, oligosaccharides,
More informationAdvances in Hybrid Mass Spectrometry
The world leader in serving science Advances in Hybrid Mass Spectrometry ESAC 2008 Claire Dauly Field Marketing Specialist, Proteomics New hybrids instruments LTQ Orbitrap XL with ETD MALDI LTQ Orbitrap
More informationSequence Identification And Spatial Distribution of Rat Brain Tryptic Peptides Using MALDI Mass Spectrometric Imaging
Sequence Identification And Spatial Distribution of Rat Brain Tryptic Peptides Using MALDI Mass Spectrometric Imaging AB SCIEX MALDI TOF/TOF* Systems Patrick Pribil AB SCIEX, Canada MALDI mass spectrometric
More informationPosterREPRINT A NOVEL APPROACH TO MALDI-TOF-MS SAMPLE PREPARATION. Presented at ABRF 2002, Austin, Texas, USA, 9th - 12th March 2002.
Introduction A NOVEL APPROACH TO MALDI-TOF-MS SAMPLE PREPARATION Ed Bouvier 2, Jeff Brown 1, Emmanuelle Claude 1, John L. Gebler 2, Weibin Chen 2, *Dominic Gostick 1, Kevin Howes 1, James Langridge 1,
More informationMS/MS to Targeted Proteomics (MRM)
MS/MS to Targeted Proteomics (MRM) How it worked on the Human Lens Proteome Jayson Falkner PhD jay@singleorganism.com Genes Show Limited Value in Predicting Diseases With only a few exceptions, what the
More informationNew Instruments and Services
New Instruments and Services http://planetorbitrap.com/orbitrap fusion Combining the best of quadrupole, Orbitrap, and ion trap mass analysis in a revolutionary Tribrid architecture, the Orbitrap Fusion
More informationFundamental Studies of Matrix-Assisted Laser Desorption/Ionization, Using Time-of-Flight Mass Spectrometry to Identify Biological Molecules
UCRL-ID-126246 Fundamental Studies of Matrix-Assisted Laser Desorption/Ionization, Using Time-of-Flight Mass Spectrometry to Identify Biological Molecules D. Eades, D. Wruck, H. Gregg November 11, 1996
More informationCharacterization of Noncovalent Complexes of Polyelectrolytes by Mass Spectrometry
The University of Akron IdeaExchange@UAkron Honors Research Projects The Dr. Gary B. and Pamela S. Williams Honors College Spring 2015 Characterization of Noncovalent Complexes of Polyelectrolytes by Mass
More informationProteomics of body liquids as a source for potential methods for medical diagnostics Prof. Dr. Evgeny Nikolaev
Proteomics of body liquids as a source for potential methods for medical diagnostics Prof. Dr. Evgeny Nikolaev Institute for Biochemical Physics, Rus. Acad. Sci., Moscow, Russia. Institute for Energy Problems
More informationProtein Identification and Phosphorylation Site Determination by de novo sequencing using PepFrag TM MALDI-Sequencing kit
Application Note Tel: +82-54-223-2463 Fax : +82-54-223-2460 http://www.genomine.com venture ldg 306 Pohang techno park Pohang, kyungbuk, Korea(ROK) Protein Identification and Phosphorylation Site Determination
More informationPhosphorylation of proteins Steve Barnes Feb 19th, 2002 in some cases, proteins are found in a stable, hyperphosphorylated state, e.g.
Phosphorylation of proteins Steve Barnes Feb 19th, 2002 in some cases, proteins are found in a stable, hyperphosphorylated state, e.g., casein more interestingly, in most other cases, it is a transient
More informationMass Spectrometry Infrastructure
Mass Spectrometry Infrastructure Todd Williams, Ph.D. Director KU Mass Spectrometry and Analytical Proteomics Laboratory Mass Spectrometry Lab B025 Malott Hall Mission The Mass Spectrometry and analytical
More informationStrategies for Characterizing Proteins in Proteomics Research
Strategies for Characterizing Proteins in Proteomics Research Joseph A. Loo Department of Biological Chemistry David Geffen School of Medicine Department of Chemistry and Biochemistry University of California
More informationProteins: Proteomics & Protein-Protein Interactions Part I
Proteins: Proteomics & Protein-Protein Interactions Part I Jesse Rinehart, PhD Department of Cellular & Molecular Physiology Systems Biology Institute DNA RNA PROTEIN DNA RNA PROTEIN Proteins: Proteomics
More informationImprove Protein Analysis with the New, Mass Spectrometry- Compatible ProteasMAX Surfactant
Improve Protein Analysis with the New, Mass Spectrometry- Compatible Surfactant ABSTRACT Incomplete solubilization and digestion and poor peptide recovery are frequent limitations in protein sample preparation
More informationRapid Detection and Identification of Bacteria in Milk Products by Matrix-Assisted Laser Desorption/Ionization Tandem Mass Spectrometry (MALDI-MS/MS)
Rapid Detection and Identification of Bacteria in Milk Products by Matrix-Assisted Laser Desorption/Ionization Tandem Mass Spectrometry (MALDI-MS/MS) Nyesa Enakaya It is essential to be able to identify
More informationMore structural information with MS n
PRODUCT SPECIFICATIONS The LTQ XL linear ion trap mass spectrometer More structural information with MS n The LTQ XL linear ion trap mass spectrometer delivers more structural information faster and with
More informationAB Sciex QStar XL. AIMS Instrumentation & Sample Report Documentation. chemistry
Mass Spectrometry Laboratory AIMS Instrumentation & Sample Report Documentation AB Sciex QStar XL chemistry UNIVERSITY OF TORONTO AIMS Mass Spectrometry Laboratory Department of Chemistry, University of
More informationOne Gene, Many Proteins. Applications of Mass Spectrometry to Proteomics. Why Proteomics? Raghothama Chaerkady, Ph.D.
Applications of Mass Spectrometry to Proteomics Raghothama Chaerkady, Ph.D. McKusick-Nathans Institute of Genetic Medicine and the Department of Biological Chemistry Why Proteomics? One Gene, Many Proteins
More informationMass spectrometry Technologies in Lipid chemistry
Mass spectrometry Technologies in Lipid chemistry Rabah Soliymani University Of Helsinki Protein Chemistry Unit Biomedicum Helsinki Rabah.soliymani@helsinki.fi Complex_&_dynamic_mixtures (few copies to
More informationMALDI-TOF Mass Spectrometry: A New Rapid ID Method in Clinical Microbiology
MALDI-TOF Mass Spectrometry: A New Rapid ID Method in Clinical Microbiology Patrick R. Murray, PhD WW Director, Scientific Affairs BD Diagnostic Systems Outline MALDI-TOF is the most important innovation
More informationLOCALISATION, IDENTIFICATION AND SEPARATION OF MOLECULES. Gilles Frache Materials Characterization Day October 14 th 2016
LOCALISATION, IDENTIFICATION AND SEPARATION OF MOLECULES Gilles Frache Materials Characterization Day October 14 th 2016 1 MOLECULAR ANALYSES Which focus? LOCALIZATION of molecules by Mass Spectrometry
More informationProtein sequence mapping is commonly used to
Reproducible Microwave-Assisted Acid Hydrolysis of Proteins Using a Household Microwave Oven and Its Combination with LC-ESI MS/MS for Mapping Protein Sequences and Modifications Nan Wang and Liang Li
More informationImpurity Identification using a Quadrupole - Time of Flight Mass Spectrometer QTOF
Impurity Identification using a Quadrupole - Time of Flight Mass Spectrometer QTOF PUSHER TOF DETECTOR ZSPRAY TM Ion Source SAMPLING CONE SKIMMER RF HEXAPOLE RF HEXAPOLE QUADRUPOLE IN NARROW BANDPASS MODE
More informationMALDI Imaging Mass Spectrometry
MALDI Imaging Mass Spectrometry Nan Kleinholz Mass Spectrometry and Proteomics Facility The Ohio State University Mass Spectrometry and Proteomics Workshop What is MALDI Imaging? MALDI: Matrix Assisted
More informationNIH Public Access Author Manuscript J Proteome Res. Author manuscript; available in PMC 2014 July 05.
NIH Public Access Author Manuscript Published in final edited form as: J Proteome Res. 2013 July 5; 12(7): 3071 3086. doi:10.1021/pr3011588. Evaluation and Optimization of Mass Spectrometric Settings during
More informationThe Detection of Allergens in Food Products with LC-MS
The Detection of Allergens in Food Products with LC-MS Something for the future? Jacqueline van der Wielen Scope of Organisation Dutch Food and Consumer Product Safety Authority: Law enforcement Control
More informationChapter 12: Mass Spectrometry: molecular weight of the sample
Structure Determination: hapter 12: Mass Spectrometry- molecular weight of the sample; formula hapter 12: Infrared Spectroscopy- indicated which functional groups are present hapter 13: Nuclear Magnetic
More informationProtein Analysis using Electrospray Ionization Mass Spectroscopy *
OpenStax-CNX module: m38341 1 Protein Analysis using Electrospray Ionization Mass Spectroscopy * Wilhelm Kienast Andrew R. Barron This work is produced by OpenStax-CNX and licensed under the Creative Commons
More informationIdentification of Haemoglobinopathies by LC/MS
Identification of Haemoglobinopathies by LC/ Mark Harrison; Senior Scientist, ThermoFisher Scientific Sarah Battle; Senior Biomedical Scientist, Royal Hallamshire Hospital Introduction There are over 2
More informationNature Methods: doi: /nmeth.3177
Supplementary Figure 1 Characterization of LysargiNase, trypsin and LysN missed cleavages. (a) Proportion of peptides identified in LysargiNase and trypsin digests of MDA-MB-231 cell lysates carrying 0,
More informationAgilent 7700x ICP-MS
Agilent 7700x ICP-MS Metals analysis Mid ppt to low ppb detection range Option to detect in four different gas modes to gain optimum detection of multiple metals or interferences in a sample Autosampler
More informationProteins. Amino acids, structure and function. The Nobel Prize in Chemistry 2012 Robert J. Lefkowitz Brian K. Kobilka
Proteins Amino acids, structure and function The Nobel Prize in Chemistry 2012 Robert J. Lefkowitz Brian K. Kobilka O O HO N N HN OH Ser65-Tyr66-Gly67 The Nobel prize in chemistry 2008 Osamu Shimomura,
More informationTerminology. Metabolite: substance produced or used during metabolism such as lipids, sugars and amino acids
Terminology Metabolite: substance produced or used during metabolism such as lipids, sugars and amino acids Metabolome: the quantitative complement of all the low molecular weight molecules present in
More informationStructural Elucidation of N-glycans Originating From Ovarian Cancer Cells Using High-Vacuum MALDI Mass Spectrometry
PO-CON1347E Structural Elucidation of N-glycans Originating From Ovarian Cancer Cells Using High-Vacuum MALDI Mass Spectrometry ASMS 2013 TP-708 Matthew S. F. Choo 1,3 ; Roberto Castangia 2 ; Matthew E.
More informationApplication Note # LCMS-89 High quantification efficiency in plasma targeted proteomics with a full-capability discovery Q-TOF platform
Application Note # LCMS-89 High quantification efficiency in plasma targeted proteomics with a full-capability discovery Q-TOF platform Abstract Targeted proteomics for biomarker verification/validation
More informationAutomated Sample Preparation/Concentration of Biological Samples Prior to Analysis via MALDI-TOF Mass Spectroscopy Application Note 222
Automated Sample Preparation/Concentration of Biological Samples Prior to Analysis via MALDI-TOF Mass Spectroscopy Application Note 222 Joan Stevens, Ph.D.; Luke Roenneburg; Tim Hegeman; Kevin Fawcett
More informationLearning Objectives. Overview of topics to be discussed 10/25/2013 HIGH RESOLUTION MASS SPECTROMETRY (HRMS) IN DISCOVERY PROTEOMICS
HIGH RESOLUTION MASS SPECTROMETRY (HRMS) IN DISCOVERY PROTEOMICS A clinical proteomics perspective Michael L. Merchant, PhD School of Medicine, University of Louisville Louisville, KY Learning Objectives
More informationQUANTITATIVE ANALYSIS IN PROTEOMICS AND METABOLOMICS
UNIVERSITA DEGLI STUDI DI NAPOLI FEDERICO II TESI DI DOTTORATO IN SCIENZE CHIMICHE XXVIII CICLO QUANTITATIVE ANALYSIS IN PROTEOMICS AND METABOLOMICS Francesca Pane Tutor Prof. Angela Amoresano Coordinatore
More informationBiomolecular Mass Spectrometry
Lipids ot different than other organic small molecules Carbohydrates Polymers of monosaccharides linked via glycosidic bonds (acetals/ ketals) many different combinationsvery interesting no time ucleic
More informationMASS SPECTROMETRY BASED METABOLOMICS. Pavel Aronov. ABRF2010 Metabolomics Research Group March 21, 2010
MASS SPECTROMETRY BASED METABOLOMICS Pavel Aronov ABRF2010 Metabolomics Research Group March 21, 2010 Types of Experiments in Metabolomics targeted non targeted Number of analyzed metabolites is limited
More informationMASS SPECTROMETRIC CHARACTERIZATION OF ELASTIN PEPTIDES AND THE EFFECT OF SOLAR RADIATION ON ELASTIN. Dissertation
MASS SPECTROMETRIC CHARACTERIZATION OF ELASTIN PEPTIDES AND THE EFFECT OF SOLAR RADIATION ON ELASTIN Dissertation zur Erlangung des akademischen Grades Doktor rerum naturalium (Dr. rer. nat.) vorgelegt
More informationAn Introduction. 1 Introduction MALDI-TOF/TOF_version100623
MALDI-TOF/TOF mass spectrometry An Introduction 1 Introduction MALDI-TOF/TOF_version100623 MALDI-TOF(/TOF) mass spectrometry MALDI ion source Time-of of-flight (TOF) mass analyzer detector 2 Introduction
More informationCharacterization of an Unknown Compound Using the LTQ Orbitrap
Characterization of an Unknown Compound Using the LTQ rbitrap Donald Daley, Russell Scammell, Argenta Discovery Limited, 8/9 Spire Green Centre, Flex Meadow, Harlow, Essex, CM19 5TR, UK bjectives unknown
More informationNature Biotechnology: doi: /nbt Supplementary Figure 1
Supplementary Figure 1 The timeline of the NGAG method for extraction of N-linked glycans and glycosite-containing peptides. The timeline can be changed based on the number of samples. Supplementary Figure
More informationBioanalytical Quantitation of Biotherapeutics Using Intact Protein vs. Proteolytic Peptides by LC-HR/AM on a Q Exactive MS
Bioanalytical Quantitation of Biotherapeutics Using Intact Protein vs. Proteolytic Peptides by LC-HR/AM on a Q Exactive MS Jenny Chen, Hongxia Wang, Zhiqi Hao, Patrick Bennett, and Greg Kilby Thermo Fisher
More informationMass Spectrometry. Actual Instrumentation
Mass Spectrometry Actual Instrumentation August 2017 See also http://www.uni-bielefeld.de/chemie/analytik/ms f additional infmation 1. MALDI TOF MASS SPECTROMETRY ON THE ULTRAFLEX 2 2. ESI MASS SPECTROMETRY
More informationMatrix Assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry
Matrix Assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry Time-of-Flight Mass Spectrometry. Basic principles An attractive feature of the time-of-flight (TOF) mass spectrometer is its
More informationChapter 3. Structure of Enzymes. Enzyme Engineering
Chapter 3. Structure of Enzymes Enzyme Engineering 3.1 Introduction With purified protein, Determining M r of the protein Determining composition of amino acids and the primary structure Determining the
More informationABSTRACT. Colin Michael Wynne, Ph.D Dr. Catherine Fenselau, Department of Chemistry and Biochemistry
ABSTRACT Title of Document: TOP-DOWN ANALYSIS OF BACTERIAL PROTEINS BY HIGH-RESOLUTION MASS SPECTROMETRY Colin Michael Wynne, Ph.D. 2010 Directed By: Dr. Catherine Fenselau, Department of Chemistry and
More informationSYNAPT G2-S High Definition MS (HDMS) System
SYNAPT G2-S High Definition MS (HDMS) System High performance, versatility, and workflow efficiency of your MS system all play a crucial role in your ability to successfully reach your scientific and business
More informationABSTRACT. Dr. Catherine Fenselau, Department of Chemistry and Biochemistry. Presented in this work is a novel bottom up proteomics approach to
ABSTRACT Title of Document: INTEGRATION OF ASP-SPECIFIC MICROWAVE-ACCELERATED ACID HYDROLYSIS INTO PROTEOMIC ANALYSES Stephen J. Swatkoski, Ph.D., 2007 Directed By: Dr. Catherine Fenselau, Department of
More informationPHOSPHOPEPTIDE ANALYSIS USING IMAC SAMPLE PREPARATION FOLLOWED BY MALDI-MS and MALDI PSD MX
PHOSPHOPEPTIDE ANALYSIS USING IMAC SAMPLE PREPARATION FOLLOWED BY MALDI-MS and MALDI PSD MX E. Claude 1, E. Emekwue 2, M. Snel 1, T. McKenna 1 and J. Langridge 1 1: Waters Corporation, Manchester, UK 2:
More informationCharacterization and Quantification of Covalent Modification of Proteins Using Mass Spectrometry
City University of New York (CUNY) CUNY Academic Works Dissertations, Theses, and Capstone Projects Graduate Center 9-2018 Characterization and Quantification of Covalent Modification of Proteins Using
More informationREDOX PROTEOMICS. Roman Zubarev.
REDOX PROTEOMICS Roman Zubarev Roman.Zubarev@ki.se Physiological Chemistry I, Department for Medical Biochemistry & Biophysics, Karolinska Institutet, Stockholm What is (RedOx) Proteomics? Proteomics -
More informationPROTEOMICS (Mass spectrometry in Biochemistry)
PROTEOMICS (Mass spectrometry in Biochemistry) A lecture combined with laboratory experiments Friedrich Altmann / Department für Chemie, Universität für Bodenkultur Wien Genomics - What is the molecular
More informationTerminology. Metabolite: substance produced or used during metabolism such as lipids, sugars and amino acids
Terminology Metabolite: substance produced or used during metabolism such as lipids, sugars and amino acids A metabolite may be described as a compound which is internalized, chemically converted or secreted
More informationOMCL Network of the Council of Europe QUALITY MANAGEMENT DOCUMENT
OMCL Network of the Council of Europe QUALITY MANAGEMENT DOCUMENT PA/PH/OMCL (10) 86 2R QUALIFICATION OF EQUIPMENT ANNEX 7: QUALIFICATION OF MASS SPECTROMETERS Full document title and reference Document
More informationMass Spectrometry a Multi-purpose Tool in Cancer Research
Mass Spectrometry a Multi-purpose Tool in Cancer Research Stephen Barnes,PhD Director, Center for Nutrient-Gene Interaction in Cancer Prevention Director, Mass Spectrometry Shared Facility Cancer Research
More informationINTRODUCTION TO MALDI IMAGING
INTRODUCTION TO MALDI IMAGING Marten F. Snel, Emmanuelle Claude, Thérèse McKenna, and James I. Langridge Waters Corporation, Manchester, UK INT RODUCTION The last few years have seen a rapid increase in
More informationPROTEOMICS (Mass spectrometry in Biochemistry)
PROTEOMICS (Mass spectrometry in Biochemistry) A lecture combined with laboratory experiments Friedrich Altmann / Department für Chemie, Universität für Bodenkultur Wien Genomics - Why? Why in English?
More informationShotgun Proteomics MS/MS. Protein Mixture. proteolysis. Peptide Mixture. Time. Abundance. Abundance. m/z. Abundance. m/z 2. Abundance.
Abundance Abundance Abundance Abundance Abundance Shotgun Proteomics Protein Mixture 1 2 3 MS/MS proteolysis m/z 2 3 Time µlc m/z MS 1 m/z Peptide Mixture m/z Block Diagram of a Mass Spectrometer Sample
More informationTime (min) Supplementary Figure 1: Gas decomposition products of irradiated DMC.
200000 C 2 CH 3 CH 3 DMC 180000 160000 140000 Intensity 120000 100000 80000 60000 40000 C 2 H 6 CH 3 CH 2 CH 3 CH 3 CCH 3 EMC DEC 20000 C 3 H 8 HCCH 3 5 10 15 20 25 Time (min) Supplementary Figure 1: Gas
More information