Molecular Analysis of the Intestinal Microflora in IBD. Gerald W. Tannock
|
|
|
- Teresa Morton
- 5 years ago
- Views:
Transcription
1 Molecular Analysis of the Intestinal Microflora in IBD Gerald W. Tannock Department of Microbiology & Immunology, University of Otago, Dunedin, New Zealand
2 Commensalism
3 The uncultured bacterial world is greater than the cultured world. Bacterial small ribosomal subunit RNA (16S rrna) or its gene. Phylogenetic signatures contained in nucleotide base sequences. ATTCGCTTCATCGCGIAATAMTTAGCBACTEROIDESTATACTGTCCG ATTCGCTTGCAACTGIGCTAMCCATACLOSTRIDIUMCATGGTGTCCG
4 Phylogenetic catalogues based on 16S rrna (gene) sequences Reference to ribosomal database project II permits phylogenetic classification of sequences. Determine biodiversity. Unclassified Bacteroidetes clone frequencies detected in association with bowel biopsies from NEWLY DIAGNOSED, UNTREATED patients (CD = 20, UC = 15) and controls (14) P < CONTROLS UC CD Clones = (63) (234) (276) GROUP Bibiloni et al., 2006, Journal of Medical Microbiology 55:
5 Fluorescence in situ hybridization (FISH)/Flow cytometry (FC) Six oligonucleotide probes that target specific sequences in 16S rrna. The probes detect phylogenetic groups of bacteria commonly present in faeces. Labeled with fluorescent dyes. FC counts the number of fluorescing cells. Bacterial groups expressed as % of total community (Eub338 probe). The six probes cover ~75% of the bowel community of healthy humans. The remaining ~25% is referred to as the phylogenetic gap.
6 Lay, C., et al Applied & Environmental Microbiology 71: %; n = 91 European adults. Kah Weng Mah et al., 2007, Pediatric Research, in press. %; n= 17 formula-fed infants. Bifidobacteria Lab Atopobium group Bacteroides- Prevotella Phylogenetic gap Clostridium cluster IV Clostridium cluster XIVa
7 Inter-subject variability = a normal range of microbiota compositions. Fecal microbiota composition determined by FISH/FACS. Ninety one healthy, European subjects. Proportions of 12 bacterial groups colorcoded.
8 Ileal pouch-anal anastomosis CP ANTIBIOTICS CP NO ANTIBIOTICS FAP NO ANTIBIOTICS n = 17 n = 15 Pouchitis - can be treated with antibiotics. Total bacterial numbers the same whether CP no antibiotic, CP antibiotic, or FAP (log per gram of stool). Patient group The phylogenetic gap is greater in pouchitis. This means that CP pouches contain bacteria not usually predominant in normal feces. Antibiotic treatment reduces the number of these bacteria (P <0.0001).
9 Community snapshots. DGGE TTGE TI RC TC LC REC TI RC TC LC REC RC TC LC REC RC TC LC REC A N In N In N In N In N In N In N In Bibiloni et al., 2006, Journal of Medical Microbiology 55: B C
10 E37-CR Bowel biopsies reveal nationality? Albertans (Canadian) Mexican n = 36 n = 62 BACTEROIDES SPECIES 74% E44-UC E16-CR E42-CR M2-H M32-UC M39-H M16-UC M17-UC M18-UC M20-UC M19-CR M21-H E64-UC M3-H M30-H M31-H M25-H M1-UC M8-UC M34-H M36-H M37-H M40-H M22-H M27-H M38-CR M23-H M26-UC M14-UC M6-UC M35-UC M9-UC M11-H M13-H E20-CR E33-CR E29-H E56-UC E62-UC E17-H E8-CR E49-UC E9-UC E11-H E7-H E50-UC E5-H E13-CR E54-CR E25-H E26-H E23-UC E28-H E30-CR E1-CR E3-H E4-H E63-UC E2-CR E21-H E12-UC E18-CR E19-UC E22-H E31-H E34-CR E36-UC E38-H E40-H E35-H E32-H E41-CR E43-CR E6-H E61-UC E45-UC E46-CR E47-UC E55-CR E57-UC E59-UC E10-H E39-H E27-CR E51-CR E53-CR E14-CR E58-UC E15-CR E52-CR E48-UC M24-UC M28-UC M33-H M15-UC M4-UC Bibiloni et al., 2007, J. Medical Microbiology in press. M29-H
11 Rodents (such as IL-10 KO) with dysfunctional immune systems as models of inflammatory diseases. SPF microbiota = disease Germfree = absence of disease Bibiloni et al., Analysis of the large bowel microbiota of colitic mice using PCR/DGGE. Letters in Applied Microbiology 41 : Mouse group
12 WT mice IL-10-deficient mice weeks after exposure to WT microbiota Bacteroides sp Lactobacillus Acidophilus group Eubacterium ventriosum Clostridium cocleatum 9 Enterococcus sp. 16 Lactobacillus murinus , 12 Bifidobacterium animalis
13 Are the bifidobacteria involved in pathogenesis? Germfree wild type 129S6/SvEv mice and IL-10-deficient mice Monoassociated with Bifidobacterium animalis subspecies animalis ATCC Histology, colon explants (IL-12) 3.5 DUO CECUM PROX COLON TRANS COLON DIST COLON 2 Colon explants 3.0 P < WT IL-10 KO 0.0 WT KO MOUSE GROUP (n = 10-19) Mouse group Moran et al., Bifidobacterium animalis causes extensive duodenitis and mild colonic inflammation in interleukin-10 knockout mice. Abstract at Digestive Diseases Week meeting.
14 Ankylosing spondylitis (AS) and bowel inflammation. Inflammation mainly of axial, particularly the sacroiliac, joints. Most patients are HLA-B27 positive. Between 5 and 10% of cases of AS are associated with IBD, either CD or UC. A much larger percentage of AS patients have subclinical gut inflammation manifested either by endoscopic findings or by histology. Rudwaleit & Baeten, 2006, Best Practice & Research Clinical Rheumatology 20:
15 HLA-B27 transgenic rats Rath et al.,1999, Infection & Immunity 67: Commensals Colitis & spondyloarthritis Germfree Absence of colitis & spondyloarthritis P <0.001
16 Clostridium cluster XIVa Atopobium group Bifidbacterium Clostridium cluster IV Bacteroides-Prevotella Patients, n = 15 P >0.05 Sulphate-reducing bacteria more persistent over three months in patients than controls. Klebsiella not detected. Stebbings et al., 2002,Rheumatology 41: Controls, n = 15
17 Comparison of B. vulgatus populations (DNA probe). 6 5 B. vulgatus as % total microbiota P > Patients Group Controls AS immune systems react differently to resident Bacteroides than controls?
18 AS and matched controls (21 of each) Stool sample Randomly pick 10 Bacteroides fragilis group cultures Lymphocyte proliferation 3 H-thymidine Blood sample Cytokines (IL-1β, IFN-γ, TNF-α, IL-6, IL-8, IL-17, IL-10)
19 AS IL IL CAS IL IL
20 IL-10 production during T-cell proliferation. Bacteroides SEA PATIENTS CONTROLS PATIENTS CONTROLS P < P >0.05 AS lack anti-inflammatory activity with regard to Bacteroides?
21 In summary - Nucleic acid-based analytical methods have potential in discriminating between microbiota of different compositions. Experimental animal and human studies point to Bacteroides as worthy of further study in IBD and related conditions, especially where the objective is to obtain functional rather than phylogenetic knowledge.
22 Supertechs: Karen Munro Mary Alice Simon Corinda Taylor Postdocs: Rodrigo Bibiloni Christophe Lay Academic associates: Margaret Baird Clinical associates: Lori Baladay Chris Daynes Richard Fedorak John Highton Robin McLeod Balfour Sartor Simon Stebbings Puneeta Tandon Mark Thompson-Fawcett Bee Wah Florencia Vargas-Voracka Sponsors: Crohns and Colitis Foundation of America. Crohns and Colitis Foundation of Canada. Lottery Grants Board HealthCare Otago Charitable Trust Otago Medical Research Foundation Otago University Research Grant Otago School of Medical Sciences/Dunedin Medical School
The enteric microbiota: Implications for IBD. Eugene B. Chang, M.D. University of Chicago
The enteric microbiota: Implications for IBD Eugene B. Chang, M.D. University of Chicago On a per cell basis, humans are mostly prokaryote 100 90 80 70 60 50 40 30 20 10 0 EuK ProK The microbial flora
Probiotics. What Should you. Know? Simon Stebbings University of Otago School of Medicine. University of Otago Rheumatology
Probiotics. What Should you Know? Simon Stebbings University of Otago School of Medicine Department of Medical and Surgical Sciences University of Otago Rheumatology Research Unit An Introduction to Probiotics
Faecalibacterium prausnitzii
Faecalibacterium prausnitzii is an anti-inflammatory commensal bacterium identified by gut microbiota analysis of Crohn disease patients PNAS 105(43): 16731-16736, 2008. Speaker: Ming-Cheng Chen Advisor:
Materials EXCLUSION CRITERIA INCLUSION CRITERIA
Bifidobacteria numbers in colon of children with inflammatory bowel disease (IBD). Gosiewski T 1, Kochan P 1,StrusM 1, Brzychczy-Wloch M 1, Drzewiecki A 1, Kowalska-Duplaga K 2, Wedrychowicz A 2, Jedynak-Wasowicz
The Gut Microbiota: Evidence For Gut Microbes as Contributors to Weight Gain
The Gut Microbiota: Evidence For Gut Microbes as Contributors to Weight Gain Michael T. Bailey, Ph.D. Center for Microbial Pathogenesis The Research Institute, Nationwide Children s Hospital Department
Understanding clinical aspects of Crohn s disease and ulcerative colitis: Implications for the basic scientist
Understanding clinical aspects of Crohn s disease and ulcerative colitis: Implications for the basic scientist Scott Plevy, MD Associate Professor of Medicine, Microbiology & Immunology UNC School of Medicine
9/18/2018. The Physiological Roles of the Intestinal Microbiota. Learning Objectives
The Physiological Roles of the Intestinal Microbiota Kelly A. Tappenden, Ph.D., R.D., FASPEN Professor and Head, Kinesiology and Nutrition University Distinguished Teacher-Scholar University of Illinois
Fecal Microbiota Transplantation for Severe sepsis and Diarrhea : a Case Report
Fecal Microbiota Transplantation for Severe sepsis and Diarrhea : a Case Report Qiurong Li Institute of General Surgery, Jinling Hospital Nanjing Univeristy Gut Microbiota 100 trillion cells 10-fold of
Azienda Ospedaliera S. Camillo Forlanini. Unità Operativa di Gastroenterologia. Moscow June Cosimo Prantera
/ Azienda Ospedaliera S. Camillo Forlanini Unità Operativa di Gastroenterologia Moscow June 2006 Cosimo Prantera ANTIBIOTICS AND BACTERIAL SPECIES Metronidazole Bacteroides - Clostridia Ciprofloxacin Escherichia
Formulations and Availability 900 BILLION 5,319 HIGH POTENCY PROBIOTIC PEDIATRIC ADULT GERIATRIC PROVEN BY RESEARCH. HIGH-POTENCY. NO SHORTCUTS.
Formulations and Availability S TU D I E S PE R D I S E A S E 39 LIVER Liver Disease, Cirrhosis, Liver Failure, Hepatic Encephalopathy S TU D I E S PE R AG E G RO U P Visbiome Regular Product Code: 693-0412-01
The role of gut microbiome in IBS
The role of gut microbiome in IBS Chung Owyang, MD H. Marvin Pollard Professor of Internal Medicine Professor of Molecular and Integrative Physiology Chief, Division of Gastroenterology Director, Pollard
The role of intestinal microbiota in metabolic disease-a novel therapeutic target.
Michael Connolly Department of Food Biosciences, The University of Reading The role of intestinal microbiota in metabolic disease-a novel therapeutic target. University of Reading 2008 www.reading.ac.uk
4/16/2018. Go with your gut: the gutjoint. Learning objectives: Disclosure of Conflicts of Interest. Collaboration: OpenBiome
Go with your gut: the gutjoint axis James T. Rosenbaum April 27, 2018 Rheumatology Research Foundation Spondylitis Association of America Disclosure of Conflicts of Interest Collaboration: OpenBiome Funding:
Microbiome GI Disorders
Microbiome GI Disorders Prof. Ram Dickman Neurogastroenterology Unit Rabin Medical Center Israel 1 Key Points Our gut microbiota Were to find them? Symbiosis or Why do we need them? Dysbiosis or when things
Research & Reviews: Journal of Microbiology and Biotechnology
Research & Reviews: Journal of Microbiology and Biotechnology e-issn:2320-3528 The Regulatory Role of Casein Glycomacropeptide (CGMP) in the Fecal Flora of Mice with Ulcerative Colitis Zhu Ming, Xueji
Stable Isotope Probing of gut bacteria RNA. Wayne Young. Identifying gut bacteria that can use sialic acid using an RNA-SIP approach
Stable Isotope Probing of gut bacteria RNA Identifying gut bacteria that can use sialic acid using an RNA-SIP approach Wayne Young Food Nutrition & Health Team Food & Bio-based Products Group AgResearch
Gut Microbiota and IBD. Vahedi. H M.D Associate Professor of Medicine DDRI
Gut Microbiota and IBD Vahedi. H M.D Associate Professor of Medicine DDRI 1393.3.1 2 GUT MICROBIOTA 100 Trillion Microbes - 10 times more than cells in our body Collective weight of about 1kg in human
Intestinal Microbiota in Health and Disease
Intestinal Microbiota in Health and Disease February 27, 2015 Master s Course in Gastroenterology Prof. Kathy McCoy 1 Overview Overview of Gut Microbiota Microbiota in Health Microbiota in Disease 2 Gut
The microbiota in spondyloarthritis. Matthew Stoll MD,PhD,MSCS University of Alabama at Birmingham June 4, 2017
The microbiota in spondyloarthritis Matthew Stoll MD,PhD,MSCS University of Alabama at Birmingham June 4, 2017 Microbiota in arthritis A youth does not get gout before sexual intercourse. Hippocrates,
What GI Physicians Need to Know About Probiotics
Transcript Details This is a transcript of an educational program accessible on the ReachMD network. Details about the program and additional media formats for the program are accessible by visiting: https://reachmd.com/programs/gi-insights/what-gi-physicians-need-to-know-about-probiotics/3844/
Bovine mastitis may be associated with the deprivation of gut Lactobacillus. C. Ma, J. Zhao, X. Xi, J. Ding, H. Wang, H. Zhang and L.Y.
Supplementary online material of Beneficial Microbes DOI: http://dx.doi.org/10.3920/bm2015.0048. Bovine mastitis may be associated with the deprivation of gut Lactobacillus C. Ma, J. Zhao, X. Xi, J. Ding,
Clinically proven to quickly relieve symptoms of common gastrointestinal disorders. TERRAGASTRO - Good health starts in the gut
Clinically proven to quickly relieve symptoms of common gastrointestinal disorders GASTROINTESTINAL DISEASE Referred to as gastrointestinal diseases, they are common disorders which affect the esophagus,
INDIVIDUAL STUDY SYNOPSIS LINPT01. EudraCT No
NAME OF SPONSOR: Lek Pharmaceuticals d.d. Verovškov 57 SI-1526 Ljubljana NAME OF FINISHED PRODUCT: Linef Forte, trde kapsule INDIVIDUAL STUDY SYNOPSIS LINPT01 EudraCT No.2009-010713-69 NAME OF ACTIVE INGREDIENTS:
Supplemental Table 1. Primers used for RT-PCR analysis of inflammatory cytokines Gene Primer Sequence
Supplemental Table 1. Primers used for RT-PCR analysis of inflammatory cytokines Gene Primer Sequence IL-1α Forward primer 5 -CAAGATGGCCAAAGTTCGTGAC-3' Reverse primer 5 -GTCTCATGAAGTGAGCCATAGC-3 IL-1β
What Have We Learned About the Microbiome in Pediatric IBD?
What Have We Learned About the Microbiome in Pediatric IBD? Ted Denson, MD Cincinnati Children s Hospital Medical Center and the University of Cincinnati College of Medicine Disclosures: NIH, CCF, AbbVie
Nature Immunology: doi: /ni Supplementary Figure 1
Supplementary Figure 1 NLRP12 is downregulated in biopsy samples from patients with active ulcerative colitis (UC). (a-g) NLRP12 expression in 7 UC mrna profiling studies deposited in NCBI GEO database.
Normal Human Flora. (Human Microbiome) Dr.Sarmad M.H. Zeiny Baghdad College of Medicine
Normal Human Flora (Human Microbiome) Dr.Sarmad M.H. Zeiny Baghdad College of Medicine 2014-2015 Objectives Describe important human normal flora. Demonstrate the epidemiology of human normal flora. Determine
Issue 3, September usio
Issue 3, September 2014 usio 1 Lactobacillus casei strain Shirota Research Update IMPROVEMENT IN INTESTINAL HEALTH 1. A probiotic fermented milk drink containing Lactobacillus casei strain Shirota improves
The Potential For Microbiome Modification In Critical Illness. Deborah Cook
The Potential For Microbiome Modification In Critical Illness Deborah Cook To review Objectives The microbiome & concepts about its modification during critical illness Interventions Predisposition to
Supplemental Fig. 1 A. C57BL/6 (B6) C. Number of colonies in culture media B6. B6 + Abx
A. Day C57BL/6 (B6) n=9 B6 + Abx (12 weeks) n=9 B. Supplemental Fig. 1 day 1 I/R 4min Sacrifice C. Number of colonies in culture media B6 Escherichia coli Lactbacillus spp. Lactobacillus murinus Enterococcus
THE ROLE OF MICROBIOME IN IBD
Disclosures THE ROLE OF MICROBIOME IN IBD Janssen UCB No relevance to the talk Subra Kugathasan, MD Professor of Pediatrics & Human Genetics Marcus Professor of Pediatric Gastroenterology Emory University
Research in IBD at University of Colorado Denver
Research in IBD at University of Colorado Denver Blair Fennimore, MD Assistant Professor of Medicine Division of Gastroenterology and Hepatology UCH Crohn s and Colitis Center Mucosal Inflammation Program
Shifts in the Intestinal Microbiota
Shifts in the Intestinal Microbiota Therapeutic Opportunities for Pre- and Probiotics? Kelly A. Tappenden, Ph.D., R.D., FASPEN Kraft Foods Human Nutrition Endowed Professor Editor-in-Chief, Journal of
Gut Lung Axis Implication of the Gut Microbiota beyond its niche
Gut Lung Axis Implication of the Gut Microbiota beyond its niche Reema Subramanian PhD Candidate (4 th year) Supervisor: Prof. Margaret Ip Department of Microbiology, CUHK Joint Graduate Student Seminar
A Case of Inflammatory Bowel Disease
A Case of Inflammatory Bowel Disease Dr Barrie Rathbone www.le.ac.uk 26 year old Polish woman Admitted as emergency under surgeons RUQ and RIF pain Abdominal pain had occurred intermittently for a few
Manipulating the gut microbiome
Manipulating the gut microbiome William DePaolo, PhD Associate Professor Medicine Director Center for Microbiome Sciences & Therapeutics University of Washington Microbiota The actual bugs that reside
New and Emerging Therapies in IBD. Sarah Streett MD, AGAF Clinical Associate Professor of Medicine Stanford University
New and Emerging Therapies in IBD Sarah Streett MD, AGAF Clinical Associate Professor of Medicine Stanford University New and Emerging Therapies in IBD I have no relevant financial disclosures. IBD is
Dietary Zinc Alters the Microbiota and Decreases Resistance to Clostridium difficile Infection
1 Dietary Zinc Alters the Microbiota and Decreases Resistance to Clostridium difficile Infection 2 3 4 5 Joseph P. Zackular 1, Jessica L. Moore 2,3, Ashley T. Jordan 1, Lillian J. Juttukonda 1, Michael
Effects of probiotics in the treatment of alcoholic hepatitis: randomized controlled multicenter study
Effects of probiotics in the treatment of alcoholic hepatitis: randomized controlled multicenter study Lactobacillus subtilis/streptococcus faecium Lactobacillus rhamnosus R0011/acidophilus R0052 Ki Tae
INFLAMMATORY BOWEL DISEASE. Jean-Paul Achkar, MD Center for Inflammatory Bowel Disease Cleveland Clinic
INFLAMMATORY BOWEL DISEASE Jean-Paul Achkar, MD Center for Inflammatory Bowel Disease Cleveland Clinic WHAT IS INFLAMMATORY BOWEL DISEASE (IBD)? Chronic inflammation of the intestinal tract Two related
Microbe-host interactions. Nov 15, 2006 Ch. 21 Science 307: , 2005.
Microbe-host interactions Nov 15, 2006 Ch. 21 Science 307:1915-20, 2005. Antimicrobial drug resistance Acquired ability to resist effects of a chemotherapeutic to which it is normally susceptible Common
Necrotizing Enterocolitis: The Role of the Immune System
Necrotizing Enterocolitis: The Role of the Immune System Patricia Denning, M.D. Associate Professor in Pediatrics Division of Neonatology Emory University School of Medicine What is NEC? What is NEC? Necrotizing
Beyond the Scope: An Integrative Gastroenterologist s Approach to Digestive Disorders
Beyond the Scope: An Integrative Gastroenterologist s Approach to Digestive Disorders Robynne Chutkan, MD, FASGE Digestive Center for Wellness, LLC MedStar Georgetown University Hospital The real voyage
Beyond the Scope: The Microbiome & The Future of Gastroenterology
Beyond the Scope: The Microbiome & The Future of Gastroenterology Robynne Chutkan, MD, FASGE Digestive Center for Wellness, LLC MedStar Georgetown University Hospital Rapid Identification of Microbes
The Intestinal Microbiota and the Developing Immune System
The Intestinal Microbiota and the Developing Immune System 4 Feb 2016 The Intestinal Microbiota 10 fold more bacterial cells than human cells 100-1000 fold more bacterial genes than human genes Why does
Page: 1 of 7. Fecal Analysis in the Diagnosis of Intestinal Dysbiosis
Page: 1 of 7 Last Review Status/Date: June 2015 Description Intestinal dysbiosis may be defined as a state of disordered microbial ecology that is believed to cause disease, including conditions such as
The number of microorganisms residing in our intestines is 10 times the number of our somatic and germ cells.
The number of microorganisms residing in our intestines is 10 times the number of our somatic and germ cells. The number of microorganisms residing in our intestines is 10 times the number of our somatic
GUT MICROBIOME WHAT IS IT? WHY IS IT IMPORTANT FOR HUMAN HEALTH?
GUT MICROBIOME WHAT IS IT? WHY IS IT IMPORTANT FOR HUMAN HEALTH? Corrie Whisner, PhD School of Nutrition and Health Promotion Arizona State University Center for Research on Ingredient Safety Annual Meeting
VITAMINS, MINERALS AND THE GUT
VITAMINS, MINERALS AND THE GUT Nutrients Looking at individual nutrients that are involved with gut health can be misleading This is not about taking individual nutrients It supports more a whole food
It Takes Guts to Grow a Brain. Josef Neu, M.D. University of Florida
It Takes Guts to Grow a Brain Josef Neu, M.D. University of Florida neuj@peds.ufl.edu The Versatile Intestine: The intestine is not only a digestive-absorptive organ Largest immune organ of the body. Harbors
Understanding probiotics and health
Understanding probiotics and health Gemma Laws MSc Student Microbiology and Immunology Department The gut microbiota The name given to the total microbial population living in our intestine Bacteria, fungi,
Gut Reaction. Mary ET Boyle, Ph. D. Department of Cognitive Science UCSD
Gut Reaction Mary ET Boyle, Ph. D. Department of Cognitive Science UCSD Ley, R. et al (2005) PNAS vol. 102 no. 31 Bacterial diversity in the distal gut (ceca) of C57BL6 mice. (A) Phylogenetic tree of
4/17/2019 DISCLOSURES OBJECTIVES GI MICROBIOME & HEALTH: A REVIEW. Nancy C. Kois, MD, FCAP Contemporary Pathology Services. There are no disclosures
GI MICROBIOME & HEALTH: A REVIEW Nancy C. Kois, MD, FCAP Contemporary Pathology Services DISCLOSURES There are no disclosures OBJECTIVES Definitions: GI microbiota, GI microbiome, probiotic, prebiotic
Project context and main objectives
Executive summary Inflammatory bowel disease (IBD) refers to the separate but related conditions Crohn s disease (CD) and Ulcerative colitis (UC). IBD can appear at any stage in life, but is becoming increasingly
!Microbiology Profile, stool
LAB #: F000000-0000-0 PATIENT: Sample Patient ID: P12345 SEX: Female AGE: 37 CLIENT #: 12345 DOCTOR: Doctor's Data, Inc. 3755 Illinois Ave. St. Charles, IL 60174!Microbiology Profile, stool BACTERIOLOGY
Microbiome: DDW 2015 update
Microbiome: DDW 2015 update Premysl Bercik (David Morgan) Farncombe Family Digestive Health Research Institute, Department of Medicine, Division of Gastroenterology, McMaster University, Hamilton Financial
Cell counting (EtBr) Before cell-lysis. Cell-lysis by 3% SDS beads beating. After cell-lysis
Key words Sample Cell counting (EtBr) DNA extraction Cell-lysis by 3% SDS beads beating Before cell-lysis After cell-lysis Cell-lysis efficiency (Maintained70%) PCR PCR amplification of partial fragments
IL-12 family members in experimental colitis. Markus F. Neurath I. Medical Clinic Johannes Gutenberg-University Mainz, Germany
IL-12 family members in experimental colitis Markus F. Neurath I. Medical Clinic Johannes Gutenberg-University Mainz, Germany IBD - Pathogenesis Genetic Predisposition Bacterial Antigens Activation of
5/2/2018 SHOULD DEEP REMISSION BE A TREATMENT GOAL? YES! Disclosures: R. Balfour Sartor, MD
5/2/218 SHOULD DEEP REMISSION BE A TREATMENT GOAL? YES! Disclosures: R. Balfour Sartor, MD Grant support for preclinical studies: Janssen, Gusto Global, Vedanta, Artizan BALFOUR SARTOR, MD DISTINGUISHED
Reduction of Population Levels of Some Indigenous Bacteria by Lactobacilli in the Gastrointestinal Tract of Gnotobiotic Rats
Microbiol. Immunol. Vol. 21 (9), 495-503, 1977 Reduction of Population Levels of Some Indigenous Bacteria by Lactobacilli in the Gastrointestinal Tract of Gnotobiotic Rats Tsugio WATANABE, Masami MOROTOMI,
The impact of the microbiome on brain and cognitive development
The Gut-Brain Axis The impact of the microbiome on brain and cognitive development Diane Stadler, PhD, RD Oregon Health & Sciences University, Portland, Oregon Lao-American Nutrition Institute With acknowledgements
MOLECULAR ANALYSIS REVEALS UNIQUE MICROBIOME IN ILEAL POUCH DURING POUCHITIS COMPARED TO HEALTHY POUCHES IN ULCERATIVE
MOLECULAR ANALYSIS REVEALS UNIQUE MICROBIOME IN ILEAL POUCH DURING POUCHITIS COMPARED TO HEALTHY POUCHES IN ULCERATIVE COLITIS AND FAMILIAL ADENOMATOUS POLYPOSIS A Thesis Presented to The Faculty of California
Sarkis K Mazmanian, California Institute of Technology
Sarkis K Mazmanian, California Institute of Technology The human microbiota The human gut harbors 10 11-10 12 bacteria per gram colonic content (>10 14 total bacteria) Total bacteria outnumber human cells
Analysis of the effect of probiotics on shaping human gut microbiota
Analysis of the effect of probiotics on shaping human gut microbiota Masahira HATTORI Center for Omics and Bioinformatics, The University of Tokyo http://www.cb.k.u-tokyo.ac.jp/hattorilab
Probiotics for Primary Prevention of Clostridium difficile Infection
Probiotics for Primary Prevention of Clostridium difficile Infection Objectives Review risk factors for Clostridium difficile infection (CDI) Describe guideline recommendations for CDI prevention Discuss
Genetics. Environment. You Are Only 10% Human. Pathogenesis of IBD. Advances in the Pathogenesis of IBD: Genetics Leads to Function IBD
Advances in the Pathogenesis of IBD: Genetics Leads to Function Pathogenesis of IBD Environmental Factors Microbes Scott Plevy, MD Associate Professor of Medicine, Microbiology & Immunology UNC School
Differences between the gut microflora of children with autistic spectrum disorders and that of healthy children
Journal of Medical Microbiology (2005), 54, 987 991 DOI 10.1099/jmm.0.46101-0 Differences between the gut microflora of children with autistic spectrum disorders and that of healthy children Helena M.
The human microbiome and how it affects heath. Nafisa M. Jadavji, PhD
The human microbiome and how it affects heath Nafisa M. Jadavji, PhD NafisaJadavji@cunet.carleton.ca Lecture Outline Housekeeping Introduction Research Initiatives to Understand Microbiome Microbiota Development
Molecular mechanisms of bacteria-host interactions at the epithelial cell level
Technical University of Munich Prof. Dr. Dirk Haller Molecular mechanisms of bacteria-host interactions at the epithelial cell level Chair for Biofunctionality Department of Food and Nutrition Science
Probiotics: Implications for Pediatric Health
Probiotics: Implications for Pediatric Health PREFACE It is no longer questioned that the interplay between our genes, nutrition, and our environment hold the keys to growth, development, and health.
Study summaries L. casei 431
This binder provides you with summaries of selected publications on Lactobacillus paracasei subsp. paracasei L. casei 431. The publications are clinical studies performed in humans documenting the effects
IBD 101. Ronen Stein, MD Assistant Professor of Clinical Pediatrics Division of Gastroenterology, Hepatology, and Nutrition
IBD 101 Ronen Stein, MD Assistant Professor of Clinical Pediatrics Division of Gastroenterology, Hepatology, and Nutrition Objectives Identify factors involved in the development of inflammatory bowel
ESPEN Congress Vienna Networking with your microbiota Specific evidence-based indications. H. Lochs (Germany)
ESPEN Congress Vienna 2009 Networking with your microbiota Specific evidence-based indications H. Lochs (Germany) Probiotics Evidence based indications H. Lochs Medizinische Klinik mit Schwerpunkt Gastroenterologie,
Digestive Disease Institute. Clinical Research. Discovering New Ways to Diagnose and Treat Digestive Diseases
Digestive Disease Institute Clinical Research Discovering New Ways to Diagnose and Treat Digestive Diseases What Is A Clinical Trial? A clinical trial is a scientific study designed to assess whether a
Microbiome as Predictor of Benefit and Toxicity in Cancer Immunotherapy
Microbiome as Predictor of Benefit and Toxicity in Cancer Immunotherapy Giuliana Magri, Ph.D Optimizing Immunotherapy New Approaches, Biomarkers, Sequences and Combinations PRBB Auditorium, Barcelona October
Microbial Adaptations of the Microbiome. Damian R. Plichta, PhD Director of Bioinformatics
Microbial Adaptations of the Microbiome Damian R. Plichta, PhD Director of Bioinformatics Tailored shotgun metagenomics Microbiome model for biomarker discovery microbiome incoming species conditional
17 th International Conference of the Inflammation Research Association: Wednesday AM (Day 4) September 9-13, 2012
17 th International Conference of the Inflammation Research Association: Wednesday AM (Day 4) September 9-13, 2012 The Sagamore Resort, Bolton Landing, NY, USA The following report includes highlights
Interaction between the gut and its microbiota in inflammatory bowel disease Sadaghian, Mehdi
University of Groningen Interaction between the gut and its microbiota in inflammatory bowel disease Sadaghian, Mehdi IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF)
Innate immune regulation of T-helper (Th) cell homeostasis in the intestine
Innate immune regulation of T-helper (Th) cell homeostasis in the intestine Masayuki Fukata, MD, Ph.D. Research Scientist II Division of Gastroenterology, Department of Medicine, F. Widjaja Foundation,
Fecal Microbiota Composition and Frailty
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Oct. 2005, p. 6438 6442 Vol. 71, No. 10 0099-2240/05/$08.00 0 doi:10.1128/aem.71.10.6438 6442.2005 Copyright 2005, American Society for Microbiology. All Rights
I n recent years, multiple studies have emphasised the role
1721 INFLAMMATORY BOWEL DISEASE Antibiotics with a selective aerobic or anaerobic spectrum have different therapeutic activities in various regions of the colon in interleukin 1 gene deficient mice F Hoentjen,
Modulating the gut microbiota for better health. SP4 Klaudyna Borewicz 30/11/2017
Modulating the gut microbiota for better health SP4 Klaudyna Borewicz 30/11/2017 Our own important Micro-cosm We are covered with microbes inside and out Microbiota research is exciting, important and
Alexander Shikhman MD, PhD Institute for Specialized Medicine July 20, 2013
Alexander Shikhman MD, PhD Institute for Specialized Medicine July 20, 2013 SPONDYLOARTHROPATHIES The spondyloarthropathy family consists of the following entities: Ankylosing spondylitis Undifferentiated
Gut Microbes: Potential targets in the prevention and treatment of IBD. Neelendu Dey, MD Instructor Division of Gastroenterology
Gut Microbes: Potential targets in the prevention and treatment of IBD Neelendu Dey, MD Instructor Division of Gastroenterology Disclosure The following are my disclosures. Potential conflicts of interest
IBD 101. Ronen Stein, MD Assistant Professor of Clinical Pediatrics Division of Gastroenterology, Hepatology, and Nutrition
IBD 101 Ronen Stein, MD Assistant Professor of Clinical Pediatrics Division of Gastroenterology, Hepatology, and Nutrition Objectives Identify factors involved in the development of inflammatory bowel
Fructo-oligosaccharides in Crohn s disease: a prospective randomised double blind controlled trial
Fructo-oligosaccharides in Crohn s disease: a prospective randomised double blind controlled trial Principal investigator: Dr James Lindsay St Bartholomew's Hospital and The Royal London Hospital Queen
Reply: Gut microbiota diversity and atopic disease: Does breast-feeding play a role?
Reply: Gut microbiota diversity and atopic disease: Does breast-feeding play a role? Thomas Abrahamsson, Hedvig E. Jakobsson, Anders F. Andersson, Bengt Bjorksten, Lars Engstrand and Maria Jenmalm Linköping
6/24/2014. How do you study microbial communities? Outnumbers Cells of the Body 10:1 Outnumber Human Genes 100:1. Michael T. Bailey, Ph.D.
Interactions between Diet and the Intestinal Microbiota: Implications for Obesity The Body is Colonized by an Enormous Array of Bacteria Outnumbers Cells of the Body 10:1 Outnumber Human Genes 100:1 Michael
Socioeconomic State & Intestinal Microbiota. Ali Keshavarzian, MD Rush University Medical Center Chicago, IL
Socioeconomic State & Intestinal Microbiota Ali Keshavarzian, MD Rush University Medical Center Chicago, IL Socioeconomic Status (SES) SES is a hierarchical social classification associated with different
Dr Adele Melton Gastroenterologist MBChB (Otago), FRACP
Dr Adele Melton Gastroenterologist MBChB (Otago), FRACP (1) Case presentation CMV in IBD (2) Prevalence (3) Treatment (4) Recommendations / Guidelines (5) Questions 60 year old male Background: UC, pancolitis
2/3/2011. Adhesion of Bifidobacterium lactis HN019 to human intestinal
PROBIOTICS LEARNING THE WHY AND WHEN PROBIOTICS DEFINITION live micro-organisms organisms that are beneficial to the host organism WHO: Live organisms which, when administered in adequate amounts, confer
New Insights on the Structure of the Human Gut Microbiota. Chaysavanh Manichanh, PhD Vall d Hebron Research Institute Barcelona
New Insights on the Structure of the Human Gut Microbiota Chaysavanh Manichanh, PhD Vall d Hebron Research Institute Barcelona Sessio Societat Catalana Malalties Infecciosas i Microbiologia March 20th,
CROHN S DISEASE AND ULCERATIVE COLITIS
Slide 1 CROHN S DISEASE AND ULCERATIVE COLITIS Slide 2 Slide 3 Crohn s Inflammation in the GI tract but usually in the lower part of the small intestines and the colon. Inflammation occur between healthy
Dysbiosis & Inflammation
MASTERING THE MICROBIOME: Dysbiosis & Inflammation 2017 Tom Fabian, PhD It is reasonable to propose that the composition of the microbiome and its activities are involved in most, if not all, of the biological
Role of the Gut Microbiota in Autoimmunity
Role of the Gut Microbiota in Autoimmunity Pavan Bhargava, MD - Neuroimmunology Fellow Division of Neuroimmunology and Neurological Infections Johns Hopkins University, Baltimore, MD. May, 2015 None Disclosures
An Emerging Trend of High Dose Probiotic Use in Clinical Practice -A Brief Survey-
-Technical Report- An Emerging Trend of High Dose Use in Clinical Practice -A Brief Survey- October 2011 The Point Institute of Nutraceutical Research is focused on examining and disseminating information
Corporate Medical Policy
Corporate Medical Policy Fecal Analysis in the Diagnosis of Intestinal Dysbiosis File Name: Origination: Last CAP Review: Next CAP Review: Last Review: fecal_analysis_for_intestinal_dysbiosis 5/2001 5/2018
Ileal Pouch Anal Anastomosis: The Preferred Method of Reconstruction after Proctocolectomy in Children
Ileal Pouch Anal Anastomosis: The Preferred Method of Reconstruction after Proctocolectomy in Children Stephanie Jones, D.O. Surgical Fellow March 21, 2011 Ulcerative Colitis Spectrum of inflammatory bowel
Examining the effects of pre and probiotics on gut microbiota during the ageing process
Session: Reviewing key ingredients shaping nutrition for healthy ageing Tuesday 22 nd November 2016 Examining the effects of pre and probiotics on gut microbiota during the ageing process Louise R Wilson
Rapid-VIDITEST. Calprotectin-Lactoferrin
Rapid-VIDITEST Calprotectin-Lactoferrin One step calprotectin and lactoferrin Card Test Instruction manual Producer: VIDIA spol. s r.o., Nad Safinou II 365, Vestec, 252 42 Jesenice, Czech Republic, Tel.: