Non-invasive sun protection factor determination using LED light
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1 Non-invasive sun protection factor determination using LED light J. Lademann, S. Schanzer, S. Kobylinski, C. Reble, G. Khazaka, G. Wiora, H. Karrer, M. C. Meinke
2 Outline 1. Introduction UV light and its effects What is the sun protection factor (SPF)? Current method for SPF determination 2. Materials and methods of our approach 3. Results of proof of principle studies 4. Conclusion and outlook on forthcoming system
3 Introduction
4 Introduction Relative spectral distribution of sun irradiation near the equator Wavelengths reaching Earth s surface: UVB: ( ) nm UVA: ( ) nm VIS: ( ) nm NIR: ( ) nm 290nm 320nm 400nm 760nm 3000nm Wavelength: Energy: 0.5% 6.3% 38.9% 54.3% Kochevar IE, Pathak MA. Dermatology in General Medicine, , 1999
5 Introduction Action spectra for erythema and for previtamin D formation in human skin UVB UVB causes e.g. Sunburn Previtamin D production Tanning (by melanin synthesis) Skin cancer Kockott D. et al. PLoS One 11: e , 2016
6 Introduction Action spectrum of the formation of free radicals in the human skin ex vivo 0,008 RG act sp = X rad / mg per mj / cm 2 0,007 0,006 0,005 0,004 UVA causes e.g. Premature skin aging Increased melanoma risk 0,003 0,002 UVA 0, Wavelength (nm) Zastrow L et al. Skin Pharmacol Physiol, 22: 31-44, 2009
7 Introduction In vivo SPF test (current standard method) 1. UVB irradiation applied at different doses 2. Evaluation of erythema 24h post irradiation 3. MED = minimal dose causing erythema 4. SPF = MED_with sunscreen MED_without sunscreen 5. >10 test subjects per sunscreen required FDA and EU call for non-invasive method
8 Introduction In vitro test Spectroscopic transmission measurement Substrate with roughness similar to skin required Poor agreement on absolute scale e.g. due to other specific properties of skin Only successfully used to measure attenuation of UVA range (UVA-PF) relativ to UVB range (SPF) if SPF is known a priori (ISO 2443:2012) => In vitro test could not replace the erythema based SPF test so far
9 Introduction Roughness of the skin picture of the skin surface 3 mm hight profile 3 mm
10 Introduction light tissue interaction Monte Carlo Simulation Distance from incident light [µm]
11 Introduction diffuse reflectance spectroscopy on skin Skin is the best substrate Transmission measurement is not possible in vivo on skin Illumination Detection Diffusely reflected light is measured Sunscreen Spatial offset of illumination and detection => Light passes sunscreen layer twice skin
12 Materials and Methods
13 Method Schematic of first functional sensor design Fiber bundle Detector 0 Detector 1 Detector 2 Fiber bundle One LED at 308nm (0.6mW, FBH)
14 Methods Study I on porcine skin Study II on human volunteers Carefully cleaning of the ear 2 mg/cm 2 cream application (4 x 4 cm 2 ) 30 min incubation time 30 measurements per area 2 mg/cm 2 cream application on the back of the volunteers Area = 10 x 10 cm 2 30 min incubation time 30 measurements per area
15 Results
16 SPF UV LED sensor Results on porcine skin y = 1,86x R² = 0,85 Very good correlation with the results from the test institutes (SPF reference) Applicable for formulations containing chemical UV-filters only or in combination with particulate filters Samples with fluorescence disturb the SPF determination SPF reference each cream was tested on 6 subjects Spectrometer is necessary Reble C, Meinke MC, Rass J et al., No more sun burn, Optik & Photonik, Volume 13, Issue 1, (2018)
17 SPF UV sensor Results porcine skin compared to human skin at the forearm y = 1,8593x R² = 0,8453 y = 0,3753x R² = 0, SPF Referenz each cream was tested on 6 subjects porcine skin human skin Comparison using three selected creams different slope compared to porcine skin Human skin shows lower backscattered signal Is the back of the volunteers a better area?
18 SPF UV LED sensor Results comparison of inner forearm and back y = 0,6092x R² = 0,9412 comparison with three creams on 6 volunteers each the back provides higher signal 20 back 10 0 Inner forearm y = 0,3753x R² = 0,8542 Measurements should be performed on the back of the volunteers SPF reference
19 SPF UV LED sensor Results on human skin at the back of volunteers y = -0,0064x 2 + 0,9971x R² = 0,8992 Very good correlation with the results from the test institutes Verification on 7 creams in total Repetition using an UVA LED is planned SPF reference Each cream was tested on 3 to 9 subjects
20 Conclusions Measurements using one LED at 308 nm showed already very good correlation to the results of the test institutes Applicable on porcine skin and human skin The back is more suitable than the forearm The method is non-invasive (no sunburn) Quick method (don t need to wait 24 hours for the erythema formation) Possible fluorescence of the cream could interfere with the backscattered light Spectrometer based set up is necessary
21 Outlook New system with spectrometer Spectrometer multi light source Spectrometer CCD Fiber coupling 77 sensor subunits
22 Outlook New light source which cover the whole UV range 8 LED simulate the sun UV radiation Composed LED spectrum
23 Acknowledgments Charité project team: Prof. Dr. Jürgen Lademann Prof. Dr. Martina Meinke Sabine Schanzer Susanna Kobylinski Dr. Maxim Darvin Cooperation partner: Courage &Khazaka Hans Karrer GmbH Freie Universität Berlin, Fachbereich Wirtschaftswissenschaft Thank you! Weitere Informationen:
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