Effects of adrenalectomy and adrenal enucleation on liquid gastric emptying in rats

Transcription

1 Brazilian Gastric emptying Journal of in Medical adrenal insufficiency and Biological Research (2000) 33: ISSN X Short Communication 1047 Effects of adrenalectomy and adrenal enucleation on liquid gastric emptying in rats M.L. Ronsini 2 and E.F. Collares 1,2 1 Departamento de Pediatria and 2 Núcleo de Medicina e Cirurgia Experimental, Faculdade de Ciências Médicas, Universidade Estadual de Campinas, Campinas, SP, Brasil Correspondence M.L. Ronsini Rua Jorge Abelin, 158, ap Santa Maria, RS Brasil mar16@zaz.com.br Research supported by CNPq. This work is part of a Master's thesis presented by M.L. Ronsini to the Departamento de Fisiologia e Biofísica, Instituto de Biologia, UNICAMP, Campinas, SP, in Publication supported by FAPESP. Received June 7, 1999 Accepted May 8, 2000 Abstract Key words Gastric emptying Adrenalectomy Adrenal enucleation Adrenal insufficiency

2 1048 M.L. Ronsini and E.F. Collares ³ a a a Table 1 - Weight gain, mean arterial pressure, hematocrit, plasma protein, glucose, sodium and potassium levels for the subgroups of rats studied. The data are reported as means ± SEM. The number of animals is given in parentheses. a P<0.05 compared to ADS; b P<0.05 compared to SHW; c P<0.05 compared to SHS (Tukey test). AD, Adrenalectomized; AE, adrenal enucleated; SH, sham operated. The third letter in each abbreviation refers to tap water (W) or 1% saline (S). SHW SHS ADS AEW AES Weight gain (%) 20.7 ± 0.8 a (33) 19.8 ± 1.0 a (36) 12.1 ± 1.2 (37) 30.0 ± 0.6 a,b,c (54) 28.0 ± 0.6 a,b,c (54) Mean arterial pressure (mmhg) ± 1.9 a (40) ± 2.4 a (39) ± 2.4 (41) ± 2.1 c (44) ± 2.4 a (39) Hematocrit (%) 46.2 ± 0.4 (10) 46.7 ± 0.4 (10) 45.6 ± 0.7 (10) 44.7 ± 0.5 (10) 45.2 ± 0.7 (10) Plasma protein (g/dl) 6.2 ± 0.1 a (10) 6.0 ± 0.1 a (10) 5.5 ± 0.1 (10) 5.5 ± 0.1 b,c (10) 5.4 ± 0.1 b,c (10) Plasma glucose (mg/dl) 70.7 ± 4.7 (10) 77.1 ± 3.6 a (10) 56.5 ± 3.1 (10) 68.5 ± 3.3 (10) 68.2 ± 5.1 (10) Plasma sodium (meq/l) ± 1.4 a (10) ± 1.8 (10) ± 1.5 (10) ± 2.4 a (10) ± 0.5 a (10) Plasma potassium (meq/l) 2.9 ± 0.1 a (10) 2.7 ± 0.1 a (10) 3.9 ± 0.2 (10) 2.5 ± 0.1 a (10) 3.0 ± 0.3 a (10)

3 Gastric emptying in adrenal insufficiency 1049 Gastric retention (%) Figure 1 - Gastric retention of three test meals (0.9% saline, 1.8% saline and 5% glucose) in adrenalectomized (AD), adrenal enucleated (AE) and sham-operated (SH) rats. The third letter in each abbreviation refers to tap water (W) or 1% saline (S). The data are reported as box plots for 10 rats/subgroup. P<0.01 (multiple comparisons test) SHW SHS ADS AEW AES SHW SHS ADS AEW AES SHW SHS ADS AEW AES Meal = 0.9% saline Meal = 1.8% saline Meal = 5% glucose

4 1050 M.L. Ronsini and E.F. Collares Acknowledgments References 1. Rodgers JB, Riley EM, Drummey GD & Isselbacher KJ (1967). Lipid absorption in adrenalectomized rats: the role of altered enzyme activity in the intestinal mucosa. Gastroenterology, 53: Oelkers WN (1996). Adrenal insufficiency. New England Journal of Medicine, 335: Zatz R (1990). A low cost tail-cuff method for estimation of mean arterial pressure in conscious rats. Laboratory Animal Science, 40: Bucaretchi F & Collares EF (1996). Effect of Phoneutria nigriventer spider venom on gastric emptying in rats. Brazilian Journal of Medical and Biological Research, 29: Belangero VMS & Collares EF (1991). Esvaziamento gástrico e acidose metabólica. I. Estudo de um modelo experimental em ratos, empregando uma solução de cloreto de amônio por via orogástrica. Arquivos de Gastroenterologia de São Paulo, 28: Leach C (1979). Tests for several independent samples - categorical explanatory variable. In: Leach C (Editor), Introduction to Statistics. A Non-Parametric Approach for the Social Sciences. John Wiley & Sons, New York, White BD, Edwards GL & Martin RJ (1996). Interaction of type I and type II corticosteroid receptor stimulation on carcass energy and carcass water. American Journal of Physiology, 270: R1099-R Collares-Buzato CB, Collares EF & Fernandes GA (1993). Effect of paraventricular nucleus lesion and cold restraint stress on gastric emptying of a liquid meal

5 Gastric emptying in adrenal insufficiency 1051 in rats. Brazilian Journal of Medical and Biological Research, 26: Lenz HJ, Raedler A, Greten H, Vale WW & Rivier JE (1988). Stress-induced gastrointestinal secretory and motor responses in rats are mediated by endogenous corticotropin-releasing factor. Gastroenterology, 95: Keller SE, Weiss JM, Schleifer SJ, Miller NE & Stein M (1983). Stress-induced suppression of immunity in adrenalectomized rats. Science, 221: Chang F-Y, Lee S-D, Yeh G-H & Wang PS (1996). Influence of the blood glucose levels on rat liquid gastric emptying. Digestive Diseases and Sciences, 41: McCann MJ & Stricker EM (1986). Gastric emptying of glucose loads in rats: effects of insulin-induced hypoglycemia. American Journal of Physiology, 251: R609- R Schvarcz E, Palmér M, Aman J & Berne C (1995). Hypoglycemia increases the gastric emptying rate in healthy subjects. Diabetes Care, 18: Schvarcz E, Pálmer M, Aman J, Horowitz M, Stridsberg M & Berne C (1997). Physiological hyperglycemia slows gastric emptying in normal subjects and patients with insulin-dependent diabetes mellitus. Gastroenterology, 113: Santos AA, Xavier-Neto J, Santiago-Jr AT, Souza MAN, Martins AS, Alzamora F & Rola FH (1991). Acute volaemic changes modify the gastroduodenal resistance to the flow of saline in anaesthetized dogs. Acta Physiologica Scandinavica, 143: Xavier-Neto J, dos Santos AA & Rola FH (1990). Acute hypervolaemia increases gastroduodenal resistance to the flow of liquid in the rat. Gut, 31: Bastl CP, Binder HJ & Hayslett JP (1980). Role of glucocorticoids and aldosterone in maintenance of colonic cation transport. American Journal of Physiology, 238: F181-F Perone MJ, Chisari AN, Gómez Dumm CL, Spinedi E & Estivariz FE (1997). Bilateral adrenal enucleation-induced changes in adenohypophyseal proopiomelanocortin (POMC)-related peptides synthesis and secretion: a comparative study with adrenalectomized rats. Journal of Endocrinological Investigation, 20: Hunt JN (1983). Mechanisms and disorders of gastric emptying. Annual Review of Medicine, 34: Stephens JR, Woolson RF & Cooke AR (1976). Osmolyte and tryptophan receptors controlling gastric emptying in the dog. American Journal of Physiology, 231: