CLINICAL/RESEAECH TRAINING AND JOB EXPERIMENCE

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1 Curriculum Vitae HONGJIE YUAN, MD, Ph.D. ADDRESS Department of Pharmacology Emory University School of Medicine 5066 Rollins Research Center 1510 Clifton Rd NE Atlanta, GA, Tel: (404) (O) (404) (Lab) Fax: (404) Yuan, Hongjie - CV May 2017 CURRENT POSITION: Assistant Professor, Dept. of Pharmacology, Emory University, SOM Deputy Director, CFERV, Center for Functional Evaluation of Rare Variants, Emory Univ. SOM EDUCATION: March, 2004 July, 1997 July, 1994 Ph.D., Akita University School of Medicine, Akita, Japan Master of Medicine, Norman Bethune Medical University, ChangChun, China MD, Baotou Medical School, Baotou, China CLINICAL/RESEAECH TRAINING AND JOB EXPERIMENCE Resident Physician, Dept. of Neurology, BaoTou Medical School Hospital, China Teaching Assistant, Dept. of Physiology, Akita University School of Medicine. Japan Research Assistant, Dept. of Physiology, Akita University School of Medicine. Japan Postdoctoral Fellow, Dr. Traynelis Lab, Dept. of Pharmacology, Emory University 2010 Observer-ship, Department of Neurology, Grady Memorial Hospital, Atlanta 2011 Certificate from ECFMG (Educational Commission for Foreign Medical Graduates) Research Associate, Dept. of Pharmacology, Emory University, School of Medicine Instructor, Dept. of Pharmacology, Emory University, School of Medicine 2015 present Assistant Professor-RT, Dept. of Pharmacology, Emory University, SOM 2017 present Deputy Director, CFERV, Center for Functional Evaluation of Rare Variants, Emory University, SOM PROFESSIONAL MEMBERSHIPS: Member, Society for Physiology, Japan Member, Society for Neuroscience, U.S Member, American Society for Biochemistry and Molecular Biology, U.S present Member, American Epilepsy Society, U.S present Member, American Society of Human Genetics, U.S. 1

2 JOUTNAL REVIEWER: 2010 Journal of Biological Chemistry, Brain Research, Steroids, Review of Future Medicine, Journal of Neurochemistry, the British Journal of Pharmacology, Molecular Pharmacology, Trends in Pharmacological Sciences, Nature Communications, Oncotarget, Human Molecular Genetics, Genes, Brain and Behavior SCHOLARSHIPS AND HONORS: College Scholarship for Excellent Students, BaoTou Medical School University Scholarship for Excellent Graduates, Norman Bethune Medical Univ Rotary Memorial Foundation Fellow 2013 Young Investigator Award, American Epilepsy Society INVITED LECTURES th Annual Meeting Northeast Physiological Soc of Japan. (Oct. 4-5, Iwate, Japan) 2012 Undiagnosed Disease Program, NIH (Host: Dr. Cornelius F. Boerkoel), Bethesda, MD 2013 Epilepsy Meeting, Pediatric Neurology, Emory Univ, Children s Healthcare of Atlanta, GA 2014 Epilepsy Center, Department of Pediatrics, Peking University First Hospital, Beijing 2014 Department of Molecular Biology, Inner Mongolia Medical University, Huhhot, China 2015 Department of Physiology, Bethune Medical School, Changchun, China 2016 Department of Pediatrics, International Hospital of Mongolian Medicine in Hohhot, China 2016 Department of Neurosurgery and Neurology, the Third Hospital of Baogang, Baotou, China th Annual Ionotropic Glutamate Receptor Retreat, McGill University, Montreal, CA 2017 The Peking University Pediatric Neurology Forum 2017 (PUPNF2017), Beijing, China RESEARCH SUPPORT 2013 HSN P Yuan (PI) 07/01/13-06/30/14, $50,000 Functional Validation of Human NMDAR mutations Identified at UDP NIH Seed Grant Program at Emory+Children s Pediatric Center Yuan (PI) 01/01/14-12/31/14, $50,000 NMDA Receptor Mutations in Childhood Idiopathic Focal Epilepsy (IFE) with Centrotemporal Spikes and Electrical Status Epilepticus in Sleep (ESES) 2014 ACTSI/URC Pilot Grant Yuan (PI) 06/01/14-05/31/15, $30,000 Molecular Mechanism of Human NMDA Receptor GRIN2B Subunit Mutations in Neurodevelopmental Disorders HSN P Yuan (PI) 09/01/14-08/31/15, $70,000 Functional Analysis of Human NMDAR and KCTD mutations Identified at UDP NIH R01HD Yuan (PI) 04/01/15-01/31/20, $1,608,579 NMDAR Mutations & Neurodevelopmental Disorder: from Mechanism to Targeted Therapy TEACHING 2012 Small Group Session in Pharmacology for MD students 2012 IBS 531 Principles of Pharmacology 2012 IBS 526 System Neuroscience 2

3 2014 IBS 6159 Human Ion Channelopathies 2016 Small Group Session in Electrophysiology for MD students 2017 Contributed Qualifying exam questions for 2017 for MSP Yuan, Hongjie - CV May 2017 STUDENT SUPERVISED 2005 present Undergraduate research: Anel Tankovic, Kevin Sheth, Hiro Kusumoto, Chun Hu, Joseph Antonio Ruiz, Yu Han Chen present Rotation students: Katie Vance, Natalie LeVasseur, Eric Armstrong; Kevin Ogden, Stefka Gyoneva, Miranda McDaniel, Lindsey Ann Shapiro Manish Karamchandani, Undergraduate Honor Thesis (with the highest honor), Department of Neuroscience and Behavioral Biology, Emory University Wenjue Chen, Emory-Xiangya Exchange Medical Student Program, 8-Year Medical Student Program, Xiangya Medical School, Central Southern University, China 2015 Joseph Pecha, SURE Program, from University of Dallas Varun Kannan, Emory University School of Medicine Discovery Phase. The 4 th -year medical student received a Discovery Phase Mini-Grant ($5,000) funded by the Atlanta Clinical and Translational Science Institute (ACTSI) Research Technologies Program. JOURNAL PUBLICATIONS (*as corresponding author): 1 Yuan H, Liu M, Huang M, Zhao LX (1998) Red nucleus reception of the blood pressure change. J Norman Bethune Univ of Med Sci 26: (in Chinese). 2 Cao G, Wang Y, Yuan H, Zhang H, Zhang M (1999) Study on the functional change and its clinical significance of the Hypothalamic-Pituitary-Adrenocortical axis of cerebral-vascular disease in different site and course. Stroke and Nervous Disorders 6: (in Chinese). 3 Cao G, Zhang M, Yuan H (2000) Effect of carotid injection of mannitol on cerebral ischemia-induced hydrocephalus in rabbit. J BaoTou Medical School 16:23-25 (in Chinese). 4 Yamada K, Ji JJ, Yuan H, Miki T, Sato S, Horimoto N, Shimizu T, Seino S, Inagaki N (2001) Protective role of ATP-sensitive potassium channels in hypoxia-induced generalized seizure. Science 292: Yamada K, Yuan H, Inagaki N (2002). Hypoxia-induced generalized seizure and ATP-sensitive potassium channels. Clinical Neuroscience 20: 483 (in Japanese). 6 Yuan H, Yamada K, Inagaki N (2004) Multiminute oscillations in mouse substantia nigra pars reticulata neurons in vitro. Neurosci Lett. 355(3): Yuan H, Yamada K, Inagaki N (2004) Glucose sensitivity in mouse substantia nigra pars reticulata neurons in vitro. Neurosci Lett. 355(3): Chen PE, Geballe MT, Stansfeld PJ, Johnston AR, Yuan H, Jacob AL, Snyder JP, Traynelis SF, Wyllie DJ (2005) Structural features of the glutamate binding site in recombinant NR1/NR2A N-methyl-D-aspartate receptors determined by site-directed mutagenesis and molecular modeling. Mol Pharmacol. 67(5): Yuan H*, Erreger K, Dravid SM, Traynelis SF (2005) Conserved structural and functional control of N-methyl-D-aspartate receptor gating by transmembrane domain M3. J Biol Chem. 280(33): (*as corresponding author) 3

4 10 Dravid SM, Erreger K, Yuan H, Nicholson K, Le P, Lyuboslavsky P, Almonte A, Murray E, Mosely C, Barber J, French A, Balster R, Murray TF, Traynelis SF (2007) Subunit-specific mechanisms and proton sensitivity of NMDA receptor channel block. J Physiol. 581(Pt 1): Lee CJ, Mannaioni G, Yuan H, Woo DH, Gingrich MB, Traynelis SF (2007) Astrocytic control of synaptic NMDA receptors. J Physiol. 581(Pt 3): Hansen KB, Yuan H, Traynelis SF (2007) Structural aspects of AMPA receptor activation, desensitization and deactivation. Curr Opin Neurobiol. 17(3): Hamill CE, Caudle WM, Richardson JR, Yuan H, Pennell KD, Greene JG, Miller GW, Traynelis SF (2007) Exacerbation of dopaminergic terminal damage in a mouse model of Parkinson's disease by the G-protein-coupled receptor protease-activated receptor 1. Mol Pharmacol. 72(3): Erreger K, Geballe MT, Kristensen A, Chen PE, Hansen KB, Lee CJ, Yuan H, Le P, Lyuboslavsky PN, Micale N, Jørgensen L, Clausen RP, Wyllie DJ, Snyder JP, Traynelis SF (2007) Subunit-specific agonist activity at NR2A-, NR2B-, NR2C-, and NR2D-containing N-methyl-D-aspartate glutamate receptors. Mol Pharmacol. 72(4): Mannaioni G, Yuan H, Lee CJ, Traynelis SF (2008) A starring role for astrocytes. Physiology News. 70: Mannaioni G, Orr AG, Hamill CE, Yuan H, Pedone KH, McCoy KL, Berlinguer Palmini R, Junge CE, Lee CJ, Yepes M, Hepler JR, Traynelis SF (2008) Plasmin potentiates synaptic N-methyl-D-aspartate receptor function in hippocampal neurons through activation of protease-activated receptor-1. J Biol Chem. 283(29): Tahirovic YA, Geballe M, Gruszecka-Kowalik E, Myers SJ, Lyuboslavsky P, Le P, French A, Irier H, Choi WB, Easterling K, Yuan H, Wilson LJ, Kotloski R, McNamara JO, Dingledine R, Liotta DC, Traynelis SF, Snyder JP (2008) Enantiomeric propanolamines as selective N-methyl-D-aspartate 2B receptor antagonists. J Med Chem. 51(18): Yuan H, Vance KM, Junge CE, Geballe MT, Snyder JP, Hepler JR, Yepes M, Low CM, Traynelis SF (2009) The serine protease plasmin cleaves the amino-terminal domain of the NR2A subunit to relieve zinc inhibition of the N-methyl-D-aspartate receptors. J Biol Chem. 284(19): Mosley CA, Myers SJ, Murray EE, Santangelo R, Tahirovic YA, Kurtkaya N, Mullasseril P, Yuan H, Lyuboslavsky P, Le P, Wilson LJ, Yepes M, Dingledine R, Traynelis SF, Liotta DC (2009) Synthesis, structural activity-relationships, and biological evaluation of novel amide-based allosteric binding site antagonists in NR1A/NR2B N-methyl-D-aspartate receptors. Bioorg Med Chem. 17(17): Yuan H*, Hansen KB, Vance KM, Ogden K, Traynelis SF (2009) Control of N-methyl-D-aspartate receptor function by the NR2 subunit amino-terminal domain. J Neurosci. 29(39): (*as corresponding author) 21 Hansen KB, Mullasseril P, Dawit S, Kurtkaya NL, Yuan H, Vance KM, Orr AG, Kvist T, Ogden KK, Le P, Vellano KM, Lewis L, Kurtkaya S, Du Y, Qui M, Murphy TJ, Snyder JP, Brauner-Osborne H, Traynelis SF (2010) Implementation of a fluorescence-based screening assay identifies histamine H3 receptor antagonists clobenpropit and iodophenpropit as 4

5 subunit-selective NMDA receptor antagonists. J Pharmacol Exp Ther. 333(3): doi: /jpet Mullasseril P, Hansen KB, Vance KM, Ogden KK, Yuan H, Kurtkaya NL, Santangelo R, Orr AG, Le P, Vellano KM, Liotta DC, Traynelis SF. (2010) A subunit-selective potentiator of NR2C- and NR2D-containing NMDA receptors. Nat Commun Oct;1(7): Acker TM, Yuan H, Hansen KB, Vance KM, Ogden KK, Jensen HS, Burger1 PB, Snyder J, Liotta DC, Traynelis SF. Expanding N-Methyl-D-Aspartate Receptor Pharmacology: A Novel Class of Non-Competitive GluN2C/D Subunit Selective Antagonist. Mol. Pharm.80(5): Burger PB, Yuan H, Karakas E, Geballe M, Furukawa H, Liotta DC, Snyder JP, Traynelis SF. (2012) Mapping the Binding of GluN2B-Selective N-Methyl-D-aspartate Receptor Negative Allosteric Modulators. Mol. Pharm. 82(2): Yuan H*, Hansen KB, Zhang J, Pierson TM, Markello TC, Fuentes Fajardo KV, Holloman CM, Golas G, Adams DR, Boerkoel CF, Gahl WA, and Traynelis SF. (2014) Functional analysis of a de novo GRIN2A missense mutation associated with early-onset epileptic encephalopathy. Nature Commun. 5:3251 doi: /ncomms4251. (*as corresponding author) 26 Hansen KB, Ogden KK, Yuan H, Traynelis SF. (2014) Distinct functional and pharmacological properties of triheteromeric GluN1/GluN2A/GluN2B NMDA receptors. Neuron, 5;81(5): doi: /j.neuron Pierson TM, Yuan H, Marsh ED, Fuentes Fajardo KV, Adams DR, Markello TC, Golas G, Simeonov DR, Holloman CM, Tankovic A, Karamchandani MM, Schreiber JM, Mullikin JC, Tifft CJ, Toro C, Boerkoel CF, Traynelis SF, Gahl WA. (2014) GRIN2A mutation and early-onset epileptic encephalopathy: personalized therapy with memantine. Ann Clin Transl Neurol. DOI: /acn Adams DR, Yuan H, Holyoak T, Arajsd KH, Hakimif P, Markello TC, Wolfe LA, Vilboux T, Burton BK, Fuentes Fajardo KV, Grahameg G, Holloman C, Sincan M, Smith ACM, Wells GA, Huang Y, Vega H, Snyder JP, Golas GA, Tifft CJ, MD, Boerkoel CF, Hanson RW, Traynelis SF, Kerr DS, Gahl WA. (2014) Three Rare Diseases in One Sib Pair: RAI1, PCK1, GRIN2B mutations associated with Smith-Magenis Syndrome, cytosolic PEPCK deficiency and NMDA receptor glutamate insensitivity. Mol Genet Metab. pii: S (14) doi: /j.ymgme Yuan H &, Myers SJ &, Wells G &, Nicholson KL, Swanger SA, Lyuboslavsky P, Tahirovic YA, Menaldino DS, Ganesh T, Wilson LJ, Liotta DC, Snyder JP, Traynelis SF. (2015) Context-Dependent GluN2B-Selective Inhibitors of NMDA Receptor Function Are Neuroprotective with Minimal Side Effects. Neuron 85(6): ( & co-first author) 30 Yuan H, Low CM, Moody OA, Jenkins A, and Traynelis SF. (2015) Ionotropic GABA and Glutamate Receptor Mutations and Human Neurological Diseases. Mol. Pharm. 88(1): doi: /mol Chen W and Yuan H* (2015) GRIN1 Mutations in Early-Onset Epileptic Encephalopathy. Pediatric Neurology Briefs, 29(6): Katzman BM, Perszyk RE, Yuan H, Tahirovic YA, Sotimehin AE, Traynelis SF, Liotta DC. (2015) A novel class of negative allosteric modulators of NMDA receptor function. Bioorg Med Chem Lett 25(23):

6 33 Li D &, Yuan H &, Ortiz-Gonzalez XR &, Marsh ED &, Tian L, McCormick EM, Kosobucki GJ, Chen W, Schulien AJ, Chiavacci R, Tankovic A, Naase C, Brueckner F, von Stülpnagel-Steinbeis C, Hu C, Kusumoto H, Hedrich UB, Elsen G, Hörtnagel K, Aizenman E, Lemke JR, Hakonarson H, Traynelis SF, Falk MJ. (2016) GRIN2D recurrent de novo mutation is an autosomal dominant cause of severe epileptic encephalopathy treatable with NMDA receptor channel blockers. Am J Hum Genet. 99(4): doi: /j.ajhg ( & co-first author) 34 Hu C, Chen W, Yuan H, Traynelis SF. Human GRIN2B Mutations in Neurodevelopmental Disorders. J Pharmacol Sci. 132(2): doi: /j.jphs Swanger SA, Chen C, Wells G, Burger PB, Tankovic A, Bhattacharya S, Strong KL, Hu C, Kusumoto H, Zhang J, Adams DR, Millichap JJ, Petrovski S, Traynelis SF,* Yuan H*. Mechanistic insight into NMDA receptor dysregulation by disease-associated rare variants in the GluN2A and GluN2B agonist binding domains. Am J Hum Genet. 99(6): doi: /j.ajhg (*co-corresponding author) 36 Ogden KK, Chen C, Swanger SA, McDaniel MJ, Fan LZ, Hu C, Tankovic A, Kusumoto H, Kosobucki GJ, Schulien AJ, Su Z, Pecha J, Bhattacharya S, Cohen AE, Aizenman E, Traynelis SF*, Yuan H*. (2017) Molecular Mechanism of Disease-Associated Mutations in the Pre-M1 Helix of NMDA Receptors and Potential Rescue Pharmacology. PLoS Genet 13(1): e doi: /journal.pgen (*co-corresponding author) 37 Gao K, Tankovic A, Zhang Y, Kusumoto H, Zhang J, Chen W, XiangWei W, Shaulsky GH, Hu C, Traynelis SF, Yuan H*, Jiang Y* (2017) A de novo loss-of-function GRIN2A mutation associated with childhood focal epilepsy and acquired epileptic aphasia. PLoS One 12(2):e doi: /journal.pone (*co-corresponding author) 38 Chen C, Tankovic A, Burger BB, Kusumoto H, Traynelis SF*, Yuan H*. (2017) Functional evaluation of a de novo GRIN2A mutation identified in a patient with profound global developmental delay and refractory epilepsy. Mol Pharmacol pii: mol doi: /mol [Epub ahead of print] (*co-corresponding author) 39 Chen W, Shieh C, Swanger SA, Tankovic A, Au M, Madan-Khetarpa S, Tagliati M, Graham J, Madan-Khetarpal S, Traynelis SF, Yuan H, Pierson TM. (2017) GRIN1 mutation associated with intellectual disability alters NMDA receptor trafficking and function. J Hum Genet MS in press. 40 Liu S, Zhou L, Yuan H, Sanz-Clemente A, Badger J, Lu W, Traynelis SF, Roche KW. (2017) C-terminus NMDA receptor missense mutations identified in human disease affect receptor localization and synaptic function. J Neurosci. MS accepted 41 Platzer K, Yuan H, Schütz H, Winschel A, Chen W, Hu C, Kusomoto H, Helbig KL, Tang S,. Laube B, Traynelis SF, Lemke JR. (2017) GRIN2B encephalopathy novel findings on phenotype, variant clustering, functional consequences and treatment. J Med Genet MS accepted BOOK CHAPTERS: 1 Yuan H and Zhang ML (1999) Neuromuscular diseases, Demyelinating diseases, and Movement Disorders. In: Neurology Eds CH Shu CH, Military Medicine Press, Beijing, p (in Chinese). 6

7 2 Dravid SM, Yuan H, Traynelis SF (2007) AMPA receptors molecular biology, pharmacology. In The New Encyclopedia of Neuroscience Eds RS Larry, Elsevier, p Yuan H, Geballe MT, Hansen KB, Traynelis SF (2008) Structure and Function of NMDA receptors. In Structural and Functional Organization of the Synapse Eds MD Ehlers and JW Hell, Springer, p Yadav R, Dravid SM, Yuan H, Traynelis SF (2017) AMPA receptors molecular biology, pharmacology. In The New Encyclopedia of Neuroscience Eds RS Larry, Elsevier, in press. ABSTRACTS: 1 Inagaki N, Ji JJ, Yuan H, Miki T, Seino S and Yamada K (2001) ABC proteins and their biochemical pathology Metabolic sensors by sulfonylurea receptors -. Annual Meeting of Japan Society for Bioscience, Biotechnology, and Agrochemistry. (Kyoto, Japan). 2 Inagaki N, Ji JJ, Yuan H, Miki T, Seino S and Yamada K (2001) The role of ATP-sensitive potassium channels in central nervous system. The 78th Annual Meeting the Japan Society of Physiology. (Mar , Kyoto, Japan). 3 Yamada K, Yuan H, Miki T, Sato S, Seino S and N. Inagaki (2001) Control of generalized seizure by ATP-sensitive potassium channels. The 78th Annual Meeting the Japan Society of Physiology. (Mar , Kyoto, Japan). 1PC35. 4 Yamada K, Ji JJ, Yuan H, Miki T, Sato S, Seino S and N. Inagaki. (2001) Control effects of ATP-sensitive potassium channels on hypoxia-induced generalized seizure. The 74th Annual Meeting the Japan Society for Biochemistry. (Oct , Kyoto, Japan). 5 Yamada K, Ji JJ, Yuan H, Miki T, Sato S, Seino S and N. Inagaki. (2001) Effect on hypoxia-induced seizure by ATP-sensitive potassium channels in substantia nigra pars reticulata. The 38th Annual Meeting the Japan society for Clinic Physiology and The 5th Meeting the Japan Society for Molecular Physiology. (Sep , Akita, Japan). 6 Inagaki N, Yuan H, Miki T, Sato S, Seino S and. Yamada K (2001) ATP-sensitive potassium channels: intracellular ATP sensor. The 24th Meeting Japan Society for Molecular Biology (Dec., Yokohama, Japan). 7 Yamada K, Ji JJ, Yuan H, Miki T, Sato S, Seino S and N. Inagaki. (2001) Protective role of ATP-sensitive potassium channels in hypoxia-induced generalized seizure. The 31st Annual Meeting Society for Neuroscience (Nov , San Diego, USA). 8 Yuan H, Yamada K and Inagaki N. (2003) The oscillatory activity in GABAergic neurons in substantia nigra pars reticulata. The 80th Annual Meeting the Japan Society for Physiology (Mar , Fukuoka, Japan) 9 Yamada K, Yuan H and Inagaki N. (2003) Spontaneous activity in GABAergic neurons in substantia nigra pars reticulata and effect of glucose. The 36th Annual Meeting of Northeast Physiological Society of Japan. (Oct. 4-5, Iwate, Japan). 10 Yuan H, Yamada K and Inagaki N. (2003) The slow oscillatory activity of spontaneous firing rate in GABAergic neurons in substantia nigra pars reticulata. The 36th Annual Meeting of Northeast Physiological Society of Japan. (Oct. 4-5, Iwate, Japan). 7

8 11 Yamada K, Yuan H, Miki T, Seino S and Inagaki N. (2003) Protective roles of KATP channels in hypoxia-induced generalized seizure. Joint Meeting, Symposium on ABC proteins and The 5th Conference on ABC Proteins and Ion Channels. From Gene to Disease-.(Jan , Kyoto, Japan). 12 Yuan H, Yamada, K and Inagaki, N. (2003) Multi-minute oscillations of neuronal firing rate in the substantia nigra pars reticulata in vitro. Joint Meeting, Symposium on ABC proteins and The 5th Conference on ABC Proteins and Ion Channels. From Gene to Disease-. (Jan , Kyoto, Japan). 13 Yuan H, Erreger K, Le P, and Traynelis SF. (2004) Covalent modification of an engineered cysteine in M3 locks open NMDA channels. The 34th Annual Meeting Society for Neuroscience (Oct , San Diego). 14 Yamada K, Yuan H, Miki T, Seino S and Inagaki N. (2005) Metabolic susceptibility of GABAergic neurons in substantia nigra pars reticulata. The 82nd Annual Meeting the Japan Society for Physiology. (May 18-10, Sendai, Japan). 15 Hamill CE, Goldshmidt A, Yuan H, Gearing M, Rees HD, Traynelis SF (2005) Expression and localization of protease-activated receptors in human basal ganglia. The 35th Annual Meeting Society for Neuroscience (Nov , Washington, DC). 16 Yuan H, Erreger K, Drvaid S, and Traynelis SF. (2005) Single Channel Properties and Differential Effect of Channel Blockers after MTSEA-Modification of A7C Mutant NMDA Receptors The 35th Annual Meeting Society for Neuroscience (Nov , Washington, DC). 17 Woo D, Yuan H, Kristensen A, Traynelis SF, Cheong E, Lee CJ (2006) Sniffer-patch quantification of Ca2+-dependent release of glutamate at submicromolar concentration from mouse cortical astrocytes. The 36th Annual Meeting Society for Neuroscience (Oct , Atlanta, GA). 18. Yuan H, Junge CE, Low C-M, Traynelis SF. (2006) Cleavage of N-terminal domain of the NR2A subunit by plasmin relieves tonic zinc inhibition of the N-methyl-D-aspartate receptor. The 36th Annual Meeting Society for Neuroscience (Oct , Atlanta, GA). 19 Yuan H, Hansen KB, and Traynelis SF. (2007) The amino terminal domain of the NR2 subunit controls channel open probability of N-methyl-D-aspartate receptors. The 37th Annual Meeting Society for Neuroscience (Nov. 3-7, San Diego, CA). 20 Yuan H, Vance KM, Junge CE, Geballe MT, Snyder JP, Hepler JR, Yepes M, Low CM, Traynelis SF. (2008) The serine protease plasmin cleaves the NR2A subunit to reduce Zn2+-inhibition of the N-methyl-D-aspartate receptor. The 38th Annual Meeting Society for Neuroscience (Nov , Washington, DC). 21 Yuan H, Hansen KB, and Traynelis SF. (2008) The amino terminal domain of the NR2 subunit controls the deactivation time course and the open probability of N-methyl-D-aspartate receptors. The 38th Annual Meeting Society for Neuroscience (Nov , Washington, DC). 22 Kvist T, Mullasseril P, Hansen KB, Kurtkaya NL, Yuan H, Vance KM, Orr1 AG, Le P, Vellano KM, Lewis I, Kurtkaya S, Du Y, Qui M, Bräuner-Osborne H, Traynelis SF. (2009) Novel NMDA receptor antagonists identified using a cell-based screening assay for allosteric modulators of NR2D-containing NMDA receptors. The 39th Annual Meeting Society for Neuroscience (Oct , Chicago, IL). 8

9 23 Yuan H, Hansen KB, Vance KM, Ogden KK, Traynelis SF. (2009) The amino terminal domain of the NR2 subunit controls activation properties of N-methyl-D-aspartate receptors. The 39th Annual Meeting Society for Neuroscience (Oct , Chicago, IL). 24 Ogden KK, Vance KM, Hansen KB, Mullasseril P, Yuan H, Traynelis SF. (2010) Mechanistic Studies of CIQ, a Novel Potentiator of GluN2C- and GluN2D-containing NMDA Receptors. The 40th Annual Meeting Society for Neuroscience (Nov , Washington, DC). 25 Yuan H, Vance KM, Sotty F, Fog K, Smith Y, Traynelis SF. (2010) NMDA receptor subunit expression in the subthalamic nucleus. The 40th Annual Meeting Society for Neuroscience (Nov , Washington, DC). 26: Khatri AH, Zimmerman SS, Yuan H, Liotta DC, Traynelis SF. (2012) GluN2C NMDA receptor potentiator. The 42nd Annual Meeting Society for Neuroscience (Oct , New Orleans, LA). 27: Yuan H, Pierson TM, Boerkoel CF, Gahl WA, and Traynelis SF. (2013) Functional changes of a de novo GRIN2A mutation in a patient with refractory seizures and early-onset epileptic encephalopathy. Investigators Workshops, AES: 67th American Epilepsy Society Annual Meeting (Dec , Washington DC). 28: Traynelis SF, Falk MJ, Yudkoff M, Hakonarson H, ; Lynch D, and Yuan H. (2014) Two NMDA Receptor Mutations in Transmembrane domain M3 Related to Early-Onset Seizures. ANA: 139th Annual Meeting American Neurological Association (Oct 12-14, Baltimore, Maryland). 29: Yuan H. Swanger SA, Wells G; Hansen KB; Adams DR, Boerkoel CF, Toro C, Gahl WA, Synder JP, and Traynelis SF. (2014) Functional Changes of a De Novo GRIN2B Missense Mutation in a Patient with Developmental Delay. ANA: 139th Annual Meeting American Neurological Association (Oct 12-14, Baltimore, Maryland). 30: Yuan H, Tankovic A, and Traynelis SF. (2014) Functional Evaluation of a De Novo GRIN2A Mutation in a Patient with CSWSS, Continuous Spike and Waves during Slow-wave Sleep Syndrome. Investigators Workshops, AES: 68th American Epilepsy Society Annual Meeting (Dec , Seattle, Washington). 31. Yuan H, Swanger S, and Traynelis SF. (2015) De novo GRIN2A mutation results in early-onset epileptic encephalopathy: from molecular mechanism to personalized therapy. The 6th FAONS Congress and 11th Biennial Conference of CNS (Sep 20-23, 2015, Tongxiang, Zhejiang, China). 32. Traynelis SF, Odgen KK, Chen W, Tankovic A, Kosobucki GJ, Schulien AJ, Hansen KB, Aizenman E, and Yuan H (2015) The human GluN2A mutation P552R enhances NMDA receptor function and promotes neurotoxicity. The 45th Annual Meeting Society for Neuroscience (Oct , 2015, Chicago, IL). 33. Pecha J, Hu C, Kusumoto H, Chen W, Yuan H, and Traynelis SF (2015) Functional Analysis of NMDAR GRIN1 Mutations Associated with Infantile-Onset Epilepsy and Encephalopathy. The 71st SWRM/67th SERMACS 2015 Conference (Nov. 4-7, 2015, Memphis, TN). 34. Yuan H, Chen W, Tankovic A, Kosobucki GJ, Odgen KK, Aizenman E, and Traynelis SF (2015) Functional Analyses of a Human GRIN2A Pre-M1 Mutation Associated with Epilepsy: from Molecular Mechanism to Rescue Pharmacology. AES: 69th American Epilepsy Society Annual Meeting (Dec , 2015, Philadelphia, PA). 9

10 35. Chen W, Tankovic A, Traynelis SF, and Yuan H (2015) Functional Evaluation of a De Novo GRIN2A Mutation Identified in a Patient with Severe Global Developmental Delay and Intractable Epilepsy. AES: 69th American Epilepsy Society Annual Meeting (Dec , 2015, Philadelphia, PA). 36. Ogden KK, Chen C, Swanger SA, McDaniel MJ, Hu C, Tankovic A, Kusumoto H, Kosobucki GJ, Schulien AJ, Su Z, Pecha J, Bhattacharya S, Cohen AE, Aizenman E, Traynelis SF, Yuan H (2016) Evaluation of NMDA receptor human mutations suggests a role for pre-m1 helix in gating. The 46th Annual Meeting Society for Neuroscience (Nov , 2016, San Diego, CA). 37. Bhattacharya S, Swanger SA, Chen C, Strong KL, Burger PB, Wells G, Tankovic A, Hu C, Kusumoto H, Millichap JJ, Traynelis SF, Yuan H (2016) Mechanistic insights into agonist binding domain mutations in NMDA receptors underlying neurodevelopmental disorders. The 46th Annual Meeting Society for Neuroscience (Nov , 2016, San Diego, CA). 38. Yuan H, Kosobucki G, Chen W, Schulien A, Tankovic A, Hu C, Kusumoto H, Li D, Ortiz-Gonzalez X, Marsh E, Falk M, Aizeman E, Traynelis SF (2016) A GRIN2D Mutation in Transmembrane Domain M3 Associated with Severe Epileptic Encephalopathy. AES: 70th American Epilepsy Society Annual Meeting (Dec , 2016, Houston, TX). 39. Kannan V, Hu C, Kusumoto H, Traynelis SF, Yuan H (2016) Molecular Mechanism of a GRIN2A M2 Mutation Associated with Early-Onset Epileptic Encephalopathy and Potential Rescue Pharmacology. AES: 70th American Epilepsy Society Annual Meeting (Dec , 2016, Houston, TX). 10