AlphaScreen TNFα Binding Assay Kit: A Homogeneous, Sensitive and High-Throughput Assay for Screening TNFα Receptors
|
|
- Tobias Owens
- 5 years ago
- Views:
Transcription
1 AlphaScreen TNFα Binding Assay Kit: A Homogeneous, Sensitive and High-Throughput Assay for Screening TNFα Receptors Bouchard N., Legault M. and Wenham D. PerkinElmer BioSignal, 1744 William, suite 3, Montréal, Québec, Canada, H3J 1R4 dean.wenham@perkinelmer.com
2 1 Abstract Tumor necrosis factor alpha (TNFα) is a cytokine involved in a wide variety of inflammatory and immunological processes. Downstream biological effects of TNFα are produced following its binding to specific cell surface receptors. Two distinct forms of TNF receptors have been identified: TNF receptor 1 (p55 or TNFR1) and TNF receptor 2 (p75 or TNFR2). The extracellular domain of TNFR1 is proteolytically cleaved to produce a soluble fragment (stnfr1) detectable in serum and urine samples. The stnfr1 acts as a physiological inhibitor of TNF analogs when it scavenges these molecules and prevents their interactions with membrane-bound receptors. Here we describe how a bead based non-radioactive, homogeneous Amplified Luminescent Proximity Homogeneous Assay (AlphaScreen ) has been developed for the screening of TNFα receptors. The AlphaScreen TNFα /stnfr1 assay kit is intended for the screening of molecules that can displace the TNFα -stnfr1 interaction and the assay employs a system where stnfr1 is captured by anti-stnfr1 Acceptor beads. The stnfr1 in turn binds to a biotinylated derivative of TNFα captured by the Streptavidin Donor beads to generate the AlphaScreen signal. Molecules binding to either TNFα or the stnfr1, at either site of interaction, will compete for binding and therefore result in a signal decrease. The AlphaScreen TNFα /stnfr1 assay kit has been optimized in order to allow assays to be performed homogeneously in 384 well plates with a total of four additions and a total of 1 hr 45 min incubation time. Pharmacological characterization of the system demonstrated a profile consistent with that described in other assays where TNFα and TNFα exhibited Ki=.1 and 4.1 nm respectively. The assay yielded a signal to background (S/B) of 2 and this as well as the affinity of TNFα were relatively unaffected by DMSO up to a concentration of 1%. Precision analysis performed in the presence of 1% DMSO revealed a highly robust assay were the difference in S/B generated between nm (bound) and 1 nm TNFα (displaced) was 15.3 with a Z of.71. In conclusion, the attributes of the AlphaScreen TNFα Binding Assay kit suggest that it is the leading technology commercially available for the HTS application of TNFα binding and further, the data from this study open up the opportunity of developing additional cytokine based AlphaScreen assays. 2
3 2 TNFα Binding Assay Principle AlphaScreen is a bead based nonradioactive Amplified Luminescent Proximity Homogenous Assay. When biological interactions bring the Donor and Acceptor beads into close proximity, reactive oxygen generated by irradiation of the Donor beads, initiates a luminescence / fluorescence cascade in the Acceptor bead. This process leads to a greatly amplified signal with light output in the nm range. When the Acceptor and Donor beads are not in close proximity, the reactive oxygen decays and only a very low background signal is generated. The AlphaScreen TNFα /stnfr1 assay kit is intended for the screening of molecules that can displace the ligand-receptor interaction. stnfr1, captured by anti-stnfr1 Acceptor bead, binds to biotinylated TNFα derivative which is captured by the Streptavidin donor beads to generate the AlphaScreen signal. Molecules binding to either TNFα or the stnfr1 will compete for binding and therefore result in a signal decrease. 3 Methods AlphaScreen TNFα assay kit (Cat# ) is available in three sizes (5, 1 and 5 assay points) and is composed of biotinylated- TNFα, anti-stnfr1 Acceptor beads, Streptavidin Donor beads and control buffer. The soluble TNFR1 is not provided in the kit. Competition assay Components were added into a 384-well white opaque plate (Corning cat# 375) as follows: 5 µl of competitor (.1 µm 1 pm final) (TNFα, PeproTech cat# 3-1A) 5 µl of stnfr1 (.3 nm final) (R&D Systems cat# 636-R1) 1 µl of anti-stnfr1 Acceptor beads (2 µg/ml final) / biotin- TNFα mix (.12 nm final) (incubate in the dark 45 minutes at 23ºC) 5 µl of Streptavidin Donor beads (2 µg/ml final) (incubate in the dark 6 minutes at 23ºC and read on AlphaQuest TM or Fusionα TM readers) Assay Buffer: 25 mm HEPES ph7.4, 25 mm NaCl and.3% Tween-2 DMSO tolerance Screening conditions were mimicked by diluting competitor (.1 µm 1 pm final) in assay buffer supplemented with DMSO (,.5, 1, 2 and 5% final) Assay performance Evaluation of the Z values were performed using the following modifications to the competition assay described above: Maximum signal is obtained with 5 µl of buffer supplemented with DMSO (1% final) Maximal displacement is obtained with 5 µl of TNFα (3 and 1 nm final) diluted in buffer supplemented with DMSO (1% final) Assay specificity Competition assays were performed with TNFα, TNFα (PeproTech cat# 3-1B) and EGF (PeproTech cat# 1-15) as competitors 3
4 4 Assay Specificity 25 AlphaScreen Signal (cps) TNFα TNFβ EGF Log [ligand (M)] The pharmacology of the binding event was characterized using TNFα, TNFβ and EGF. IC 5 values obtained for TNFα and TNFβ were.27 nm and 4.52 nm respectively. These values are in agreement with data obtained by other groups using different detection technologies. Concentrations of EGF up to.1 µm had no effect on the system. The assay was performed on 3 separate occasions, representative data are shown. 5 DMSO Tolerance 2 [DMSO] AlphaScreen Signal (cps) %.5% 1% 2% 5% [DMSO] (%) IC 5 (nm) S/B Log [TNFα (M)] The tolerance of the assay to DMSO was tested in competition assays. DMSO concentration dependently decreased the signal. At 1% DMSO, the signal and pharmacology where both acceptable and thus suitable for screening. 4
5 6 Assay Performance 2 Total TNFα at 3 nm TNFα at 1 nm AlphaScreen Signal (cps) # well TNFα (nm) (Total) Mean Signal (cps) 149 Standard Deviation (cps) 1166 CV (%) Z nm (IC7) nm (NS) The Z (in the presence of 1% DMSO) was estimated to determine the screening suitability of an assay. A total of 96 wells for each conditions (total binding, IC 7 displacement and maximum displacement) were performed on 2 separate plates. 7 Conclusions A homogeneous and non-radioactive assay to screen compounds acting specifically to inhibit the TNFα /stnfr1 interaction has been developed. This simple assay can be performed with only 4 successive reagent additions and completed within 2 hours. Data from pharmacological characterization is in agreement with data obtained by other groups using radioactive technologies. Different sources of receptor can be used. Using the recommended assay protocol, Z >.5 in the presence of 1% DMSO can be achieved indicative of a performance that is suitable for HTS applications. Data from this study open up the opportunity of developing additional cytokinebased AlphaScreen assays. 5
6 PerkinElmer Life and Analytical Sciences 71 Bridgeport Avenue Shelton, CT USA Phone: (8) or (+1) For a complete listing of our global offices, visit 23 PerkinElmer, Inc. All rights reserved. The PerkinElmer logo and design are registered trademarks of PerkinElmer, Inc. (PKI product names) are trademarks and (PKI product names) are registered trademarks of PerkinElmer, Inc. or its subsidiaries, in the United States and other countries. All other trademarks not owned by PerkinElmer, Inc. or its subsidiaries that are depicted herein are the property of their respective owners. PerkinElmer reserves the right to change this document at any time and disclaims liability for editorial, pictorial or typographical errors. 6939_2 73 Printed in USA
Utilizing AlphaLISA Technology to Screen for Inhibitors of the CTLA-4 Immune Checkpoint
APPLICATION NOTE AlphaLISA Technology Authors: Matthew Marunde Stephen Hurt PerkinElmer, Inc. Hopkinton, MA Utilizing AlphaLISA Technology to Screen for Inhibitors of the CTLA-4 Immune Checkpoint Introduction
More informationImproved Stability of the LANCE Ultra Signal in Kinase Assays
Improved Stability of the LANCE Ultra Signal in Kinase Assays LANCE Ultra is a high throughput screening (HTS) technology platform optimized for homogeneous time-resolved fluorescence resonance energy
More informationFor Research Use Only
LANCE Ultra camp Kit For Research Use Only 1. Intended use The LANCE Ultra camp kit is intended for the quantitative determination of 3,5 -cyclic monophosphate (camp) in cell culture and cellular membrane
More informationAlphaScreen Nickel Chelate Donor Beads at 5 mg/ml in 25 mm Hepes, ph 7.4, 100 mm
Technical Data Certificate of Analysis Caution: For Laboratory Use. Research chemicals for research purposes only. AlphaScreen Nickel Chelate Donor Beads PRODUCT NUMBERS: AS101D (1 mg) AS101M (5 mg) AS101R
More informationAlphaScreen : A Straightforward and Powerful Alternative to ELISA. Martina Bielefeld-Sévigny Ph.D., R&D Director
AlphaScreen : A Straightforward and Powerful Alternative to ELISA Martina Bielefeld-Sévigny Ph.D., R&D Director Overview AlphaScreen - an alternative to ELISA Why an alternative to ELISA? Assay principle
More informationHuman Immunodeficiency Virus type-1 p24 protein (HIV p24) Kit
TECHNICAL DATA SHEET AlphaLISA Research Reagents Caution: For Laboratory Use. A research chemical for research purposes only. Human Immunodeficiency Virus type-1 p24 protein (HIV p24) Kit Product No.:
More informationAlphaLISA Human Insulin kit
Technical Data Certificate of Analysis Caution: For Laboratory Use. Research chemicals for research purposes only. AlphaLISA Insulin Kit PRODUCT NUMBERS: AL204C (500 ASSAY POINTS) AL204F (5 000 ASSAY POINTS)
More informationMouse / Rat Connecting Peptide (m / r C-Peptide) Kit
TECHNICAL DATA SHEET AlphaLISA Research Reagents Caution: For Laboratory Use. A research chemical for research purposes only. Mouse / Rat Connecting Peptide (m / r C-Peptide) Kit Product No.: AL510 C/F
More informationHuman C-C Motif Chemokine 5 / Regulated Upon Activation Normal T-cell Expressed and Secreted (CCL5 / RANTES) Kit
TECHNICAL DATA SHEET AlphaLISA Research Reagents Caution: For Laboratory Use. A research chemical for research purposes only. Human C-C Motif Chemokine 5 / Regulated Upon Activation Normal T-cell Expressed
More informationHLA DRA (alpha chain) and LAG-3 (Human) Binding AlphaLISA Kit
TECHNICAL DATA SHEET AlphaLISA Research Reagents Research Use Only. Not for use in diagnostic procedures. HLA DRA (alpha chain) and LAG-3 (Human) Binding AlphaLISA Kit Product No.: AL3066 Contents Product
More informationHepatitis B Virus Surface antigen (HBsAg) AlphaLISA Detection Kit
TECHNICAL DATA SHEET AlphaLISA Research Reagents Research Use Only. Not for use in diagnostic procedures. Hepatitis B Virus Surface antigen (HBsAg) AlphaLISA Detection Kit Product No.: AL3083 HV/C/F Contents
More informationPD1/PD-L1 BINDING ASSAY KITS
PD1/PD-L1 BINDING ASSAY KITS PROTOCOL Part # 64ICP01PEG & 64ICP01PEH Test size: 500 tests (64ICP01PEG), 10,000 tests (64ICP01PEH) - assay volume: 20 µl Revision: 02 (July 2017) Store at: -60 C or below
More informationCaution: For Laboratory Use. A research chemical for research purposes only.
TECHNICAL DATA SHEET AlphaLISA Research Reagents Caution: For Laboratory Use. A research chemical for research purposes only. Human Insulin Kit Product No.: AL204 C/F Lot No.: 647-429-A Material Provided
More informationHuman Glucagon-Like Peptide-1 (7-36 amide) (GLP-1 (7-36 amide)) Kit
TECHNICAL DATA SHEET AlphaLISA Research Reagents Caution: For Laboratory Use. A research chemical for research purposes only. Human Glucagon-Like Peptide-1 (7-36 amide) (GLP-1 (7-36 amide)) Kit Product
More informationCTLA-4 (Human) AlphaLISA Detection Kit
TECHNICAL DATA SHEET AlphaLISA Research Reagents Research Use Only. Not for use in diagnostic procedures. CTLA-4 (Human) AlphaLISA Detection Kit Product No.: AL3050 Contents Product Information... 2 Quality
More informationInfluenza A NP AlphaLISA Detection Kit
TECHNICAL DATA SHEET AlphaLISA Research Reagents Research Use Only. Not for use in diagnostic procedures. Influenza A NP AlphaLISA Detection Kit Product No.: AL3084C/F Contents Product Information... 2
More informationMouse C-C Motif Chemokine 5 / Regulated Upon Activation Normal T-Cell Expressed, and Secreted (mccl5/rantes) Kit
TECHNICAL DATA SHEET AlphaLISA Research Reagents Caution: For Laboratory Use. A research chemical for research purposes only. Mouse C-C Motif Chemokine 5 / Regulated Upon Activation Normal T-Cell Expressed,
More informationAlphaLISA Total Ghrelin (Human) Detection Kit
TECHNICAL DATA SHEET AlphaLISA Research Reagents AlphaLISA Total Ghrelin (Human) Detection Kit Product number: AL3012 HV/C/F Lot number: Sample Manufacturing date: August 26, 2016 Caution: For Laboratory
More informationAlphaLISA Thyroid-Stimulating Hormone (TSH, Human) Detection Kit
TECHNICAL DATA SHEET AlphaLISA Research Reagents AlphaLISA Thyroid-Stimulating Hormone (TSH, Human) Detection Kit Product number: AL3005 Lot number: Sample Manufacturing date: August 16, 2016 Caution:
More informationINSULIN MOUSE SERUM KITS
INSULIN MOUSE SERUM KITS PROTOCOL Part # 62IN3PEF & 62IN3PEB Test size#: 200 tests (62IN3PEF), 5 x 200 tests (62IN3PEB) - assay volume: 20 µl Revision: 03-Jan.2018 Store at: 2-8 C (62IN3PEF); 2-8 C (62IN3PEB)
More informationConcentration Estimation from Flow Cytometry Exosome Data Protocol
Concentration Estimation from Flow Cytometry Exosome Data Protocol 1. STANDARD CURVE Create a standard curve for the target exosome by plotting the mean fluorescence (y axis) against the protein concentration
More informationAlphaLISA HIV P24 Biotin-Free Detection Kit
TECHNICAL DATA SHEET AlphaLISA Research Reagents AlphaLISA HIV P24 Biotin-Free Detection Kit Product number: AL332 HV/C/F Caution: For Laboratory Use. A research product for research purposes only. Contents
More informationSERUM GLUCAGON KITS PROTOCOL
SERUM GLUCAGON KITS PROTOCOL Part # 62SGLPEB & 62SGLPEF Test size#: 5 x 200 tests (62SGLPEB), 200 tests (62SGLPEF) - assay volume: 20 µl Revision: 02-Jan.2018 Store at: -60 C or below (62SGLPEB); -60 C
More informationFor the rapid, sensitive and accurate detection of Cathepsin D inhibition by various compounds
ab126779 Cathepsin D Inhibitor Screening Kit Instructions for Use For the rapid, sensitive and accurate detection of Cathepsin D inhibition by various compounds This product is for research use only and
More informationCisbio Bioassays MAP-Tau assay is only intended for quantitative measurement of microtubule-associated protein tau (MAP-Tau) using HTRF technology.
MAP-TAU KITS PROTOCOL Part # 63ADK008PEG & 63ADK008PEH Test size#: 500 tests (63ADK008PEG), 10,000 tests (63ADK008PEH) - assay volume: 20 µl Revision: 02-Jan.2018 Store at: -20 C or below (63ADK008PEG);
More informationQS S Assist KINASE_ADP-Glo TM Kit
QS S Assist KINASE_ADP-Glo TM Kit Description KINASE ADP-Glo TM kit is designed for use in biochemical kinase assays based on a Luminescent ADP Detection Assay (ADP-Glo TM ). The kit includes human kinase,
More informationFor the rapid, sensitive and accurate measurement of Glucose in various samples
ab65333 Glucose Assay Kit Instructions for Use For the rapid, sensitive and accurate measurement of Glucose in various samples This product is for research use only and is not intended for diagnostic use.
More informationcolorimetric sandwich ELISA kit datasheet
colorimetric sandwich ELISA kit datasheet For the quantitative detection of human TNF-alpha in serum, plasma and cell culture supernatants. general information Catalogue Number Product Name Species cross-reactivity
More informationab SREBP-2 Translocation Assay Kit (Cell-Based)
ab133114 SREBP-2 Translocation Assay Kit (Cell-Based) Instructions for Use For analysis of translocation of SREBP-2 into nuclei. This product is for research use only and is not intended for diagnostic
More informationab Exosome Isolation and Analysis Kit - Flow Cytometry, Cell Culture (CD63 / CD81)
Version 1 Last updated 26 September 2018 ab239682 Exosome Isolation and Analysis Kit - Flow Cytometry, Cell Culture (CD63 / For the isolation and analysis of exosome from cell culture. This product is
More informationGlucose Detection Kit
ab102517 Glucose Detection Kit Instructions for Use For the rapid, sensitive and accurate measurement of Glucose levels in various samples This product is for research use only and is not intended for
More informationReliable. results. efficiently. for CH, galactosemia, CAH and CF screening
GSP Neonatal assays for CH, galactosemia, CAH and CF screening Reliable results efficiently GSP Neonatal kits DELFIA or enzyme-based fluorescence assays GSP is the new, automated neonatal screening system
More informationcolorimetric sandwich ELISA kit datasheet
colorimetric sandwich ELISA kit datasheet For the quantitative detection of rat TNF-alpha concentrations in cell culture supernatants. general information Catalogue Number Product Name Species cross-reactivity
More informationProduct # R8132 (Explorer Kit) R8133 (Bulk Kit)
Product Insert QBT Fatty Acid Uptake Assay Kit Product # R8132 (Explorer Kit) R8133 (Bulk Kit) Introduction About the Fatty Acid Uptake Assay Kit The homogeneous QBT Fatty Acid Uptake Assay Kit from Molecular
More informationGlucose Assay Kit. Catalog Number KA assays Version: 07. Intended for research use only.
Glucose Assay Kit Catalog Number KA0831 100 assays Version: 07 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information... 4 Materials Supplied...
More informationProduct information: Human Apolipoprotein B kit 10,000 tests 1. ASSAY DESCRIPTION AND INTENDED USE 2. PROTOCOL AT GLANCE
Headquarters & Europe Office Cisbio Bioassays Phone: +33 ()4 66 79 67 5 Fax: +33 ()4 66 79 19 2 bioassays@cisbio.com USA Office Cisbio US, Inc. Phone: +1 888 963 4567 Fax: +1 781 687 15 htrfinfo@cisbio.us
More informationA Membrane Receptor Binding Assay Using MesoScale Electrochemiluminescence Technology
A Membrane Receptor Binding Assay Using MesoScale Electrochemiluminescence Technology Jian Huang, Jay Liu and Robert Bostwick Lead Discovery Department AstraZeneca Pharmaceuticals Background The receptor
More informationab Membrane fluidity kit Instructions for Use For the detection of membrane fluidity in cells
ab189819 Membrane fluidity kit Instructions for Use For the detection of membrane fluidity in cells This product is for research use only and is not intended for diagnostic use. Version 1 Last Updated
More informationAlkaline Phosphatase Assay Kit (Fluorometric)
Alkaline Phosphatase Assay Kit (Fluorometric) Catalog Number KA0820 500 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information...
More informationAcid Phosphatase Assay Kit (Fluorometric)
ab83370 Acid Phosphatase Assay Kit (Fluorometric) Instructions for Use For the rapid, sensitive and accurate measurement of Acid Phosphatase in various samples This product is for research use only and
More informationData Sheet. PCSK9[Biotinylated]-LDLR Binding Assay Kit Catalog # 72002
Data Sheet PCSK9[Biotinylated]-LDLR Binding Assay Kit Catalog # 72002 DESCRIPTION: The PCSK9[Biotinylated]-LDLR Binding Assay Kit is designed for screening and profiling purposes. PCSK9 is known to function
More informationCathepsin K Activity Assay Kit (Fluorometric)
ab65303 Cathepsin K Activity Assay Kit (Fluorometric) Instructions for Use For the rapid, sensitive and accurate measurement of Cathepsin K activity in various samples. This product is for research use
More informationGlucose Oxidase Assay Kit (Fluorometric)
ab138884 Glucose Oxidase Assay Kit (Fluorometric) Instructions for Use For quantifying Glucose oxidase in solution. This product is for research use only and is not intended for diagnostic use. Table of
More informationFree Fatty Acid Uptake Assay Kit (Fluorometric)
ab176768 Free Fatty Acid Uptake Assay Kit (Fluorometric) Instructions for Use For measurement of fatty acid uptake in cells containing fatty acid transporters. This product is for research use only and
More information20S Proteasome Activity Assay Kit
20S Proteasome Activity Assay Kit For 100 Assays Cat. No. APT280 FOR RESEARCH USE ONLY NOT FOR USE IN DIAGNOSTIC PROCEDURES USA & Canada Phone: +1(800) 437-7500 Fax: +1 (951) 676-9209 Europe +44 (0) 23
More informationEpiQuik HDAC Activity/Inhibition Assay Kit (Fluorometric)
EpiQuik HDAC Activity/Inhibition Assay Kit (Fluorometric) Base Catalog # PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE The EpiQuik HDAC Activity/Inhibition Assay Kit (Fluorometric) is very suitable for
More informationMyeloperoxidase (MPO) Activity Assay Kit (Fluorometric)
ab111749 Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric) Instructions for Use For the rapid, sensitive and accurate measurement of Myeloperoxidase activity in various samples. This product is for
More informationFor the accurate measurement of glucose in cells and tissues, plasma, serum and other body fluids, growth media and food.
ab169559 Picoprobe Glucose Assay Kit Instructions for Use For the accurate measurement of glucose in cells and tissues, plasma, serum and other body fluids, growth media and food. This product is for research
More informationab Exosome Isolation and Analysis Kit - Flow Cytometry, Cell culture
Version 1 Last updated 14 March 2018 ab228564 Exosome Isolation and Analysis Kit - Flow Cytometry, Cell culture For the measurement of human exosomes in cell culture. This product is for research use only
More informationFor the accurate measurement of glycogen levels in various biological samples.
ab169558 Glycogen Assay Kit II (Colorimetric) Instructions for Use For the accurate measurement of glycogen levels in various biological samples. This product is for research use only and is not intended
More informationGlucose Uptake Colorimetric Assay Kit
ab136955 Glucose Uptake Colorimetric Assay Kit Instructions for Use For the sensitive and accurate measurement of Glucose uptake in various samples This product is for research use only and is not intended
More informationRayBio Human ENA-78 ELISA Kit
RayBio Human ENA-78 ELISA Kit Catalog #: ELH-ENA78 User Manual Last revised April 15, 2016 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite 100 Norcross,
More informationSupporting Information
Supporting Information Burford et al. 1.173/pnas.1339311 SI Materials and Methods β-arrestin Recruitment Assay. PathHunter human osteosarcoma cells (U2OS) expressing either μ-opioid receptors (U2OS- OPRM1)
More informationData Sheet. PCSK9[Biotinylated]-LDLR Binding Assay Kit Catalog # 72002
Data Sheet PCSK9[Biotinylated]-LDLR Binding Assay Kit Catalog # 72002 DESCRIPTION: The PCSK9[Biotinylated]-LDLR Binding Assay Kit is designed for screening and profiling purposes. PCSK9 is known to function
More informationHTRF MEASUREMENT OF CYTOKINE RELEASE FROM FRESH BLOOD SAMPLES
HTRF MEASUREMENT OF CYTOKINE RELEASE FROM FRESH BLOOD SAMPLES APPLICATION NOTE ABSTRACT Cisbio offers a comprehensive line of HTRF cytokine assays to investigate the functional response of immune cells.
More informationCaution: For Laboratory Use. A product for research purposes only. Eu-W1024 ITC Chelate & Europium Standard. Product Number: AD0013
TECHNICAL DATA SHEET Lance Caution: For Laboratory Use. A product for research purposes only. Eu-W1024 ITC Chelate & Europium Standard Product Number: AD0013 INTRODUCTION: Fluorescent isothiocyanato-activated
More informationL-Amino Acid Assay Kit
ab65347 L-Amino Acid Assay Kit Instructions for Use For the rapid, sensitive and accurate measurement of L-Amino Acid levels in various samples. This product is for research use only and is not intended
More informationab MitoTox Complex I OXPHOS Activity Microplate Assay
ab109903 MitoTox Complex I OXPHOS Activity Microplate Assay Instructions for Use For the quantitative measurement of Complex I activity in Bovine samples This product is for research use only and is not
More informationGlucose and Sucrose Assay Kit
ab65334 Glucose and Sucrose Assay Kit Instructions for Use For the rapid, sensitive and accurate measurement of Glucose and Sucrose levels in various samples This product is for research use only and is
More informationFor the rapid, sensitive and accurate measurement of Lactose levels in various samples
ab83384 Lactose Assay Kit Instructions for Use For the rapid, sensitive and accurate measurement of Lactose levels in various samples This product is for research use only and is not intended for diagnostic
More informationRayBio Human Thyroglobulin ELISA Kit
RayBio Human Thyroglobulin ELISA Kit Catalog #: ELH-Thyroglobulin User Manual Last revised July 6, 2017 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite
More informationMeasuring PD-L1 Expression in Breast Cancer Cell Lines with AlphaLISA
APPLICATION NOTE AlphaLISA Technology Author: Jeanine Hinterneder, PhD PerkinElmer Inc. Hopkinton, MA Measuring PD-L1 Expression in Breast Cancer Cell Lines with AlphaLISA Introduction In the tumor microenvironment,
More informationData Sheet. Fluorogenic HDAC 8 Assay Kit Catalog #: 50068
Data Sheet Fluorogenic HDAC 8 Assay Kit Catalog #: 50068 DESCRIPTION: The Fluorogenic HDAC 8 Assay Kit is a complete assay system designed to measure histone deacetylase (HDAC) 8 activity for screening
More informationab PAF Acetylhydrolase Inhibitor Screening Assay Kit
ab133091 PAF Acetylhydrolase Inhibitor Screening Assay Kit Instructions for Use For screening PAF Acetylhydrolase inhibitors. This product is for research use only and is not intended for diagnostic use.
More informationab HRP Substrate Instructions for Use
ab155473 HRP Substrate Instructions for Use For detecting the activity of many oxidases, related enzymes/substrates or cofactors such as glucose, acetylcholine and cholesterol, L-glutamate, amino acids
More informationCaution: For Laboratory Use. A product for research purposes only. Eu-W1284 Iodoacetamido Chelate & Europium Standard. Product Number: AD0014
TECHNICAL DATA SHEET Lance Caution: For Laboratory Use. A product for research purposes only. Eu-W1284 Iodoacetamido Chelate & Europium Standard Product Number: AD0014 INTRODUCTION: Iodoacetamido-activated
More informationQuantifying Changes in CD28 and CTLA-4 Levels in Peripheral Blood Mononuclear Cells with
APPLICATION NOTE AlphaLISA Technology Author: Matthew Marunde PerkinElmer, Inc. Hopkinton, MA Quantifying Changes in CD28 and CTLA-4 Levels in Peripheral Blood Mononuclear Cells with AlphaLISA Technology
More informationab Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma
Version 1 Last updated 25 May 2018 ab228565 Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma For the isolation/detection of exosomes from human plasma, urine or cell culture media. This product
More informationFor the rapid, sensitive and accurate measurement of Maltose levels in various samples.
ab83388 Maltose Assay Kit Instructions for Use For the rapid, sensitive and accurate measurement of Maltose levels in various samples. This product is for research use only and is not intended for diagnostic
More informationFocus Application. Compound-Induced Cytotoxicity
xcelligence System Real-Time Cell Analyzer Focus Application Compound-Induced Cytotoxicity Featured Study: Using the Time Resolving Function of the xcelligence System to Optimize Endpoint Viability and
More informationab ATP Synthase Enzyme Activity Microplate Assay Kit
ab109714 ATP Synthase Enzyme Activity Microplate Assay Kit Instructions for Use For the quantitative measurement of ATP Synthase activity in samples from Human, Rat and Cow This product is for research
More informationLipase Detection Kit III (Fluorometric)
ab118969 Lipase Detection Kit III (Fluorometric) Instructions for Use For the rapid, sensitive and accurate measurement of Lipase activity in various samples. This product is for research use only and
More informationab83375 Sialic Acid (NANA) Assay Kit Instructions for Use For the rapid, sensitive and accurate measurement of Sialic Acid (NANA) in various samples.
ab83375 Sialic Acid (NANA) Assay Kit Instructions for Use For the rapid, sensitive and accurate measurement of Sialic Acid (NANA) in various samples. Version 2 Last Updated 4 March 2015 This product is
More informationEPIGENTEK. EpiQuik HDAC Activity/Inhibition Assay Kit(Colorimetric) Base Catalog # P-4002 PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE
EpiQuik HDAC Activity/Inhibition Assay Kit(Colorimetric) Base Catalog # PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE The EpiQuik HDAC Activity/Inhibition Assay Kit (Colorimetric) is very suitable for
More informationD-Mannitol Colorimetric Assay Kit
ab155890 D-Mannitol Colorimetric Assay Kit Instructions for Use For the sensitive and accurate measurement of D-Mannitol/L-Arabitol in various plant tissues/cells and food products. This product is for
More informationHuman Glucagon-Like Peptide-1 (7-36 amide)(glp-1 (7-36 amide)) LANCE Ultra Detection Kit
TECHNICAL DATA SHEET LANCE Ultra Research Reagents Human Glucagon-Like Peptide-1 (7-36 amide)(glp-1 (7-36 amide)) LANCE Ultra Detection Kit Caution: For Laboratory Use. A research product for research
More informationHuman Utrophin 500 tests
Headquarters & Europe Office Cisbio Bioassays Phone: +33 (0)4 66 79 67 05 Fax: +33 (0)4 66 79 19 20 bioassays@cisbio.com USA Office Cisbio US, Inc. Phone: +1 888 963 4567 Fax: +1 781 687 1500 htrfinfo@cisbio.us
More informationAscorbic Acid Assay Kit
Ascorbic Acid Assay Kit Catalog Number KA1660 100 assays Version: 04 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Principle of the Assay... 3 General
More informationcolorimetric sandwich ELISA kit datasheet
colorimetric sandwich ELISA kit datasheet For the quantitative detection of human IL5 in serum, plasma, cell culture supernatants and urine. general information Catalogue Number Product Name Species cross-reactivity
More informationFor the rapid, sensitive and accurate measurement of Sucrose levels in various samples
ab83387 Sucrose Assay Kit Instructions for Use For the rapid, sensitive and accurate measurement of Sucrose levels in various samples This product is for research use only and is not intended for diagnostic
More informationLipase Detection Kit II (Colorimetric)
ab102525 Lipase Detection Kit II (Colorimetric) Instructions for Use For the rapid, sensitive and accurate measurement of Lipase activity in various samples. This product is for research use only and is
More informationab Myeloperoxidase (MPO) Inhibitor Screening Assay Kit
ab133080 Myeloperoxidase (MPO) Inhibitor Screening Assay Kit Instructions for Use The Myeloperoxidase (MPO) Inhibitor Screening Assay provides convenient fluorescence-based methods for screening inhibitors
More informationSupplementary Data 1. Alanine substitutions and position variants of APNCYGNIPL. Applied in
Supplementary Data 1. Alanine substitutions and position variants of APNCYGNIPL. Applied in Supplementary Fig. 2 Substitution Sequence Position variant Sequence original APNCYGNIPL original APNCYGNIPL
More informationSUPPLEMENTAL INFORMATION
SUPPLEMENTAL INFORMATION EXPERIMENTAL PROCEDURES Tryptic digestion protection experiments - PCSK9 with Ab-3D5 (1:1 molar ratio) in 50 mm Tris, ph 8.0, 150 mm NaCl was incubated overnight at 4 o C. The
More informationab Histone Deacetylase (HDAC) Activity Assay Kit (Fluorometric)
ab156064 Histone Deacetylase (HDAC) Activity Assay Kit (Fluorometric) Instructions for Use For the quantitative measurement of Histone Deacetylase activity in cell lysates This product is for research
More informationab Homocysteine Assay Kit (Fluorometric) 1
Version 1 Last updated 25 May 2018 ab208559 Homocysteine Assay Kit (Fluorometric) For the measurement of homocysteine in mammalian plasma and serum. This product is for research use only and is not intended
More informationab Dipeptidyl peptidase IV (DPP4) Inhibitor Screening Assay Kit
ab133081 Dipeptidyl peptidase IV (DPP4) Inhibitor Screening Assay Kit Instructions for Use For screening DPP4 inhibitors. This product is for research use only and is not intended for diagnostic use. 1
More informationMouse serum Insulin 200 tests
Headquarters & Europe Office Cisbio Bioassays Phone: +33 (0)4 66 79 67 05 Fax: +33 (0)4 66 79 19 20 bioassays@cisbio.com cisbio_dd_pi_62in3pef-2µl USA Office Cisbio US, Inc. Phone: +1 888 963 4567 Fax:
More informationab65311 Cytochrome c Releasing Apoptosis Assay Kit
ab65311 Cytochrome c Releasing Apoptosis Assay Kit Instructions for Use For the rapid, sensitive and accurate detection of Cytochrome c translocation from Mitochondria into Cytosol during Apoptosis in
More informationA Homogeneous Phosphoinositide 3-Kinase Assay on Phospholipid FlashPlate Platforms. Busi Maswoswe, Hao Xie, Pat Kasila and Li-an Yeh
A Homogeneous Phosphoinositide 3-Kinase Assay on Phospholipid FlashPlate Platforms Busi Maswoswe, Hao Xie, Pat Kasila and Li-an Yeh Abstract Phosphoinositide 3-kinases (PI 3-kinase) consist of a family
More informationQIAsymphony DSP Circulating DNA Kit
QIAsymphony DSP Circulating DNA Kit February 2017 Performance Characteristics 937556 Sample to Insight Contents Performance Characteristics... 4 Basic performance... 4 Run precision... 6 Equivalent performance
More informationab Cell Invasion Assay (Basement Membrane), 24-well, 8 µm
Version 1 Last updated 29 June 2018 ab235882 Cell Invasion Assay (Basement Membrane), 24-well, 8 µm For the measurement of cell invasion in response to stimuli. This product is for research use only and
More informationCONVENTIONAL VACCINE DEVELOPMENT
CONVENTIONAL VACCINE DEVELOPMENT PROBLEM Lethal germ Dead mouse LIVE VACCINES Related but harmless germ gives protection against lethal pathogen. Examples are the original pox vaccine and some TB vaccines
More informationab Intracellular O 2 Probe
ab140101 Intracellular O 2 Probe Instructions for Use For the measurement of intracellular oxygen in populations of live mammalian cells This product is for research use only and is not intended for diagnostic
More informationab Lipid Peroxidation (MDA) Assay kit (Colorimetric/ Fluorometric)
Version 10b Last updated 19 December 2018 ab118970 Lipid Peroxidation (MDA) Assay kit (Colorimetric/ Fluorometric) For the measurement of Lipid Peroxidation in plasma, cell culture and tissue extracts.
More informationL-Amino Acid Assay Kit
ab65347 L-Amino Acid Assay Kit Instructions for Use For the rapid, sensitive and accurate measurement of L-Amino Acid levels in various samples. This product is for research use only and is not intended
More information