Transcription

1 Rate ressure roduct dp/dt ().

2 KUMS.REC

3 NaHCO3 KCl CaCl2 NaCl ANOVA MgSO4 KH2O4 Glucose Tukey ost Test H Graphpad Instat, version 3.05 AD Instrument, MLT pressure transducer Bridge Amp New south Wales, Australia AD Instrument, New ML ower Lab South Wales, Australia : Left Ventricular Developed ressure Rate ressure roduct (±dp/dt)

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5 IGF-1 β -MHC α -MHC (Allicin and Alliin) II

6 References 1- Nahrendorf M, Frantz S, Hu K, et al. Effect of testosterone on post-myocardial infarction remodeling and function. Cardiovasc Res. 2003; 57: Bissoli NS, Santos Medeiros AR, Silva Santos MC, et al. Long-term treatment with supraphysiological doses of nandrolone decanoate reduces the sensitivity of Bezold Jarisch reflex control of heart rate and blood pressure. harmacol Res. 2009; 59: enna C, Tullio F, errelli M-G, et al. Ischemia/reperfusion injury is increased and cardioprotection by a postconditioning protocol is lost as cardiac hypertrophy develops in nandrolone treated rats. Basic Res Cardiol. 2011; 106: apamitsou Th, Barlagiannis D, apaliagkas V, Kotanidou E, Dermentzopoulou-Theodoridou M. Testosterone-induced hypertrophy, fibrosis and apoptosis of cardiac cells an ultrastructural and immunohistochemical study. Med Sci Monit. 2011; : Longo DL, Fauci AS, Kasper DL, Hauser SL, Jameson J, Loscalzo J. Harrison's rinciples of Internal Medicine. New York, NY: McGraw-Hill; Cavasin MA, Sankey SS, Yu A-L, MenonSh, Yang X. Estrogen and testosterone have opposing effects on chronic cardiac remodeling and function in mice with myocardial infarction. AM J hysiol-heart Circ hysiol. 2003; 284: Cavasin MA, Tao ZY, Yu AL, Yang X. Testosterone enhances early cardiac remodeling after myocardial infarction, causing rupture and degrading cardiac function. AM J hysiol-heart Circ hysiol. 2006; 290: Grace F, Sculthrope N, Baker J, Davies B. Blood pressure and rate pressure product response in males using high-dose anabolic androgenic steroids (AAS). J Sci Med Sport. 2003; 6: Du Toit EF, Rossouw E, Van Rooyen J, Lochner A. roposed mechanisms for the anabolic steroid-induced increase in myocardial susceptibility to ischaemia/reperfusion injury. Cardiovasc J S Afr. 2005; 1: Wang M, Tsai BM, Kher A, Baker LB, Wairiuko GM, Meldrum DR. Role of endogenous testosterone in myocardial proinflammatory and proapoptotic signaling after acute ischemiareperfusion. AM J hysiol-heart Circ hysiol. 2005; 288:

7 11- Sadowska-Krepa E, Klapcinska B, Jagsz S, et al. High-dose testosterone propionate treatment reverses the effects of endurance training on myocardial antioxidant defenses in adolescent male rats. Cardiovasc Toxicol. 2011; 11: hillis BD, Abeywardena MY, Adams MJ, Kennedy JA, Irvine RJ. Nandrolone potentiates arrhythmogenic effects of cardiac ischemia in the rat. Toxicol Sci. 2007; 99: Belhani D, Fanton L, Vaillant F, et al. Cardiac lesions induced by testosterone: protective effects of dexrazoxane and trimetazidine. Cardiovasc Toxicol. 2009; 9: Crisostomo R, Wang M, Wairiuko GM, Morrell ED, Meldrum DR. Brief exposure to exogenous testosterone increases death signaling and adversely affects myocardial function after ischemia. Am J hysiol Regul Integr Comp hysiol. 2006; 290: Hara Y, Noda A, Miyata S, et al. Effects of aged garlic extract on left ventricular diastolic function and fibrosis in a rat hypertension model. Exp Anim. 2013; 62: Liu Ch, Cao F, Tang QZh, et al. Allicin protects against cardiac hypertrophy and fibrosis via attenuating reactive oxygen species-dependent signaling pathways. J Nutr Biochem. 2010; 21: Al-Qattan KK, Khan I, Alnaqeeb MA, Ali M. Mechanism of garlic (Allium sativum) induced reduction of hypertension in 2K-1C rats: a possible mediation of Na/H exchanger isoform-1. rostag Leukot Essnt Fatty Acids. 2003; 69: Louis XL, Murphy R, Thandapilly SJ, Yu L, Netticadan T. Garlic extracts prevent oxidative stress, hypertrophy and apoptosis in cardiomyocytes: a role for nitric oxide and hydrogen sulfide. BMC Complement Altern Med. 2012; 12: 140: Banerjee SK, Maulik SK. Effect of garlic on cardiovascular disorders: a review. Nutr J, 2002; 1: 4: Chang SH, Liu CJ, Kuo CH, et al. Garlic oil alleviates MAKs- and IL-6-mediated diabetes-related cardiac hypertrophy in STZinduced DM rats. Evid Based Complement Alternat Med. 2011; : Shackebaei D, Feizollahi F, Hesari M, Bahrami GH. The effect of diazepam on the function of hypertrophied rats hearts in ischemiareperfusion conditions. Int Cardiovasc Res J. 2016; 10: Shackebaei D, Vaezi M, Hesari M, Asadmobini A. The direct effect of diazepam on the cardiac parameters (left ventricular developed pressure, heart rate and coronary solution flow) in isolated hypothyroid rat heart under schemia reperfusion conditions. J Zanjan Univ Med Sci, 2013; 21: Frnatz S, HU K, Widder J, et al. Detrimental effects of testosterone on

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9 rotective Effects of Garlic owder on the erformance of Testosterone- Induced Cardiac Hypertrophy in Rats Shackebaei D 1, Gorgani R 2, Hesari M 1, Bahrami GH 1, Kiani A 3 1 Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran 2 Faculty of harmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran 3 Tissue Engineering and Regenerative Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran Corresponding Author: Hesari M, Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran mahvashhesari@gmail.com Received: 29 Dec 2016 Accepted: 28 May 20 Background and Objective: The androgens cause the cardiac hypertrophy and cardiomyopathy. There are reports of garlic protective effects in the cardiac hypertrophy due to overload and catecholamine. The aim of this study was to investigate the effects of garlic powder on testosterone-induced cardiac hypertrophy in rats. Materials and Methods: This experimental study was performed on Wistar male rats in 5 groups (n = 7-8) including the testosterone group (10 mg testosterone enanthate for 20 days), the garlic group (1.6 g for 10 days), the control garlic group and finally the garlic + testosterone. The separated hearts were perfused according to Langendorff method and passed three basal, ischemia and reperfusion stages. The structural and functional parameters of left ventricular fluctuations (), ventricular contractility (dp / dt) and rate pressure product () were measured. Results: In the testosterone-induced hypertrophy group, the cardiac weight, the thickness of the left ventricular wall and the functional parameters of the heart, such as,, + dp / dt, and -dp / dt indicated significant increase. The use of garlic powder in the base period led to a significant decrease in these parameters ( <0.05). However, in the period of ischemic reperfusion, the effect was not significant. Conclusion: The results showed that the garlic powder modifies the cardiac hypertrophy induced by testosterone administration in rats. In other words, the use of garlic powder could potentially reduce the severity of cardiac hypertrophy in rats, due to variations in functional and structural parameters. Key words: Garlic, Cardiac Hypertrophy, Testosterone, Ischemia / Reperfusion, Rat