SPERM HY-LITER TM. Monoclonal Antibody-Based Fluorescent Detection of Human Sperm from Sexual Assault Evidence. Jack Keehma Austin TX February 1, 2008
|
|
|
- Valentine Patrick
- 5 years ago
- Views:
Transcription
1 Monoclonal Antibody-Based Fluorescent Detection of Human Sperm from Sexual Assault Evidence Jack Keehma Austin TX February 1, 2008
2 Introduction to Independent Forensics Forensic DNA laboratory Accreditations: ISO 17025, NY-DOH, AABB, CLIA Specialize in LCN / Trace Protocols Profiles from < 0.1 ng Products for Forensic Laboratories Contract R & D Laboratory personnel: MS and Ph.D. scientists 5,600 sq ft of combined laboratory and office space Suburban Chicago 18 minutes from O Hare 30 minutes from The Loop
3 History of Project R & D contract from Federal Law Enforcement Agency Lateral Flow Immunochromatographic Strip Tests RSID TM -SEMEN RSID TM -SALIVA RSID TM -BLOOD Fluorescent Detection of Sperm from Sexual Assault evidence SPERM PLUS TM
4 Test Format: Microscopic Screening Method for Sperm Antigen Detected: Antibodies: Human Sperm Heads Mouse monoclonal Labeling: Sperm: Alexa 488 / Nuclei: DAPI Sensitivity: Cross-reaction: Single Human Sperm Head None detected Test Read-out: Visual Preparation Time: 100 minutes 10 minutes hands-on
5 Smear Slide Staining Protocol Sexual Assault Smear Slide Magic Hydrophobic Barrier Ring Marker Phase DAPI FITC Wash Sol n Step 1: Step 2: Fix Permeabilize 10 min Sample Preparation 30 min Wash Sol n Step 3: Blocking Solution 30 min Wash Sol n Step 4: Sperm Staining 30 min Stained, Mounted Cover Slipped Slide Wash Sol n Step 5: Mounting Media 1 min
6 Phase Contrast staining Cell Type Specificity Buccal Extract, Smear slide with Sperm DAPI Filter FITC Filter
7 Phase Contrast View staining Cell Type Specificity Post-Coital Sample, Smear slide with Sperm DAPI Filter FITC Filter
8 Phase Contrast SPERM PLUS TM staining Cell Type Specificity Buccal, Urine & Blood extracts without Sperm DAPI Filter FITC Filter
9 Phase Contrast staining Cell Type Specificity Buccal, Urine & Blood extracts with Sperm DAPI Filter FITC Filter
10 Phase Contrast staining Sensitivity & Specificity Buccal, Urine & Blood Extracts Down to a Single Sperm DAPI Filter FITC Filter
11 Phase Contrast staining Species Specificity Animal Semen (various) without Human Semen DAPI Filter FITC Filter
12 Phase Contrast staining Species Specificity Animal Semen (various) with Human Semen DAPI Filter FITC Filter
13 staining Archived KPIC stained slides? Stain with SPERM, See what you have missed!
14 Dual Color Fluorescence Sperm and Epithelial Cells Simultaneously
15 staining Dual Color Fluorescence With Automation
16 SPERM PLUS TM Demonstrated: Cell type specificity only sperm cells labeled Species specificity only human sperm labeled Sensitivity a single sperm is easily visible Ease of use smear slides or cutting extracts easily stained Hidden sperm are stained and quickly identified
17 SPERM PLUS TM First Positive Identification for Human Sperm First Specific Identification for Human Sperm Sensitivity down to a single sperm Stain over KPIC: See what you have missed! Scan slides at lower magnification: 100X or 200X
18 SPERM PLUS TM HY-LITES: Stain over KPIC confirm sperm identification Dual Cube see both sperm & epithelial cells Automation scan up to 8 slides w/o operator intervention McCrone Microscope package comes with on-site training and installation
19 Acknowledgements: SPERM PLUS TM Jennifer Old, Ph.D. Brett Schweers, Ph.D. P.W. Boonlayangoor, Ph.D. Chicago Liz Graffy, MS Marisa Farhner, M.S
20 Test For Current Issue/Problem Semen p30/psa specificity / sensitivity Blood hemoglobin specificity Saliva amylase activity specificity Sperm KPIC / morphology specificity / sensitivity Test For New Issue/Problem Semen RSID-SEMEN TM semenogelin Solved Blood RSID-BLOOD TM glycophorin A Solved Saliva RSID-SALIVA TM α-amylase Better Sperm sperm head Solved
21 Questions Comments Samples Gripes Jack Keehma Tel Fax
Independent Forensics 4600 West Roosevelt Road Suite 201 Hillside IL 60162, USA. Telephone: (708) Fax: (708)
Developmental validation of SPERM HY-LITER A Specific, Sensitive and Confirmatory Microscopic Screening Method for the Detection of Human Sperm from Sexual Assault Evidence Independent Forensics 4600 West
DEVELOPMENTAL VALIDATION OF SPERM HY-LITER EXPRESS Jennifer Old, Chris Martersteck, Anna Kalinina, Independent Forensics, Lombard IL
DEVELOPMENTAL VALIDATION OF SPERM HY-LITER EXPRESS Jennifer Old, Chris Martersteck, Anna Kalinina, Independent Forensics, Lombard IL Background and Introduction SPERM HY-LITER is the first immunofluorescent
1.0 Purpose - This procedure specifies the method for conducting analysis for semen and sperm in forensic casework.
Procedure for Semen and Sperm Analysis 1.0 Purpose - This procedure specifies the method for conducting analysis for semen and sperm in forensic casework. 2.0 Scope - This procedure applies to those Forensic
RSID-Rapid Stain Test
RSID-Rapid Stain Test Prices valid in Germany 10. April 2015 RSID SINGLE BUFER SYSTEM Laboratory Kits: 0130 RSID SALIVA single buffer 25 Tests/Kit 0230 RSID SEMEN single buffer 25 Tests/Kit 0330 RSID BLOOD
Body Fluid ID by Mass Spectrometry
Body Fluid ID by Mass Spectrometry Kevin Legg, PhD & Phillip Danielson, PhD This research was supported in part by DNA R&D Grants 2006-DN-BX-K001, 2009-DN-BX-K165, 2011-CD-BX-0205, and 2012-DN-BX-K035
The Magic Erase-her The Erase Sperm Isolation Kit in DNA Casework. Shena Latcham Missouri State Highway Patrol Crime Lab Jefferson City
The Magic Erase-her The Erase Sperm Isolation Kit in DNA Casework Shena Latcham Missouri State Highway Patrol Crime Lab Jefferson City Meet the Erase Sperm Isolation Kit Purpose: More effectively and efficiently
Developmental Validation Studies of RSID TM -Saliva Lateral Flow Immunochromatographic Strip test for the forensic detection of Saliva
Developmental Validation Studies of RSID TM -Saliva Lateral Flow Immunochromatographic Strip test for the forensic detection of Saliva Dr. Jennifer Old, Dr. Brett Schweers, Dr. P.W. Boonlayangoor and Dr.
DNA TECHNIQUES AVAILABLE FOR USE IN FORENSIC CASE WORK
DNA TECHNIQUES AVAILABLE FOR USE IN FORENSIC CASE WORK FOR FORENSIC ASSISTANCE 24 HOURS A DAY, 7 DAYS A WEEK 0800 FORENSIC 0800 367 367 z KEY CONTACT Dr Stephen Cordiner Forensic Manager Service Centres
Forensic Serology. Forensic Science
Forensic Serology Forensic Science The Composition of Blood A complex mixture of cells, enzymes, proteins, and inorganic substances It mainly consists of Erythrocytes = red blood cells (rbc) Leukocytes
The Composition of Blood
Forensic Serology 1 The Composition of Blood A complex mixture of cells, enzymes, proteins, and inorganic substances. It mainly consists of Erythrocytes = red blood cells (rbc) Leukocytes = white blood
The author(s) shown below used Federal funds provided by the U.S. Department of Justice and prepared the following final report:
The author(s) shown below used Federal funds provided by the U.S. Department of Justice and prepared the following final report: Document Title: Author: Development of an Automated System to Detect Spermatozoa
Developmental Validation of a Novel Lateral Flow Strip Test for Rapid Identification of Human Blood, Rapid Stain Identification Blood, RSID -Blood
Developmental Validation of a Novel Lateral Flow Strip Test for Rapid Identification of Human Blood, Rapid Stain Identification Blood, RSID -Blood Introduction Blood is the most common body fluid encountered
HER2 FISH pharmdx TM Interpretation Guide - Breast Cancer
P A T H O L O G Y HER2 FISH pharmdx TM Interpretation Guide - Breast Cancer For In Vitro Diagnostic Use FDA approved as an aid in the assessment of patients for whom Herceptin TM (trastuzumab) treatment
System overview Installation System Description System Default Settings and Loading I-Button Tests Components/Kits /Accessories Maintenance & Cleaning
SQA-VISION TRAINING Content of Presentation System overview Installation System Description System Default Settings and Loading I-Button Tests Components/Kits /Accessories Maintenance & Cleaning SQA-Vision
Dako IT S ABOUT TIME. Interpretation Guide. Agilent Pathology Solutions. ALK, ROS1 and RET IQFISH probes (Dako Omnis) MET IQFISH probe (Dako Omnis)
INTERPRETATION Dako Agilent Pathology Solutions IQFISH Interpretation Guide ALK, ROS1 and RET IQFISH probes (Dako Omnis) MET IQFISH probe (Dako Omnis) IT S ABOUT TIME For In Vitro Diagnostic Use ALK, ROS1,
M.D.IPA, M.D.IPA Preferred, Optimum Choice and Optimum Choice Preferred STAT Laboratory List Revised Jan. 5, 2017
M.D.IPA, M.D.IPA Preferred, Optimum Choice and Optimum Choice Preferred STAT Laboratory List Revised Jan. 5, 2017 If laboratory results are required on a STAT basis, the designated commercial medical laboratory
An Alternative to the Human Hemoglobin Test in the Investigation of Bloodstains Treated with Active Oxygen: The Human Glycophorin A Test
Research Article TheScientificWorldJOURNAL (2011) 11, 907 916 ISSN 1537-744X; DOI 10.1100/tsw.2011.89 An Alternative to the Human Hemoglobin Test in the Investigation of Bloodstains Treated with Active
Law Enforcement Moving Beyond The Sexual Assault Kit. Assistant Chief Mary Lentschke Houston Police Department
The Sexual Assistant Chief Mary Lentschke Houston Police Department Law Perspective Law Perspective Background Law Perspective Background Project research Law Perspective Background Project research Role
TECHNICAL GUIDANCE FOR THE ACCREDITATION OF ANDROLOGY LABORATORIES
TECHNICAL GUIDANCE FOR THE ACCREDITATION OF ANDROLOGY LABORATORIES Approved By: Chief Executive Officer: Ron Josias Accreditation Executive: Mpho Phaloane Revised By: Medical Specialist Technical Committee
Serology. Murder & Mayhem. Crimes/evidence & Forensic Serology 2/5/2013. FRNSC 100 Dr. Jeni Smith
Serology FRNSC 100 Dr. Jeni Smith Murder & Mayhem Out damned spot! Out, I say Here's the smell of the blood still, All the perfumes of Arabia will not Sweeten this little hand. Oh, Oh, Oh! Shakespeare
HER2 CISH pharmdx TM Kit Interpretation Guide Breast Cancer
P A T H O L O G Y HER2 CISH pharmdx TM Kit Interpretation Guide Breast Cancer FROM CERTAINTY COMES TRUST For in vitro diagnostic use HER2 CISH pharmdx Kit HER2 CISH pharmdx Kit is intended for dual-color
EXCELLENCE IN FORENSIC BIOLOGY BODILY FLUIDS TEST KITS LABORATORY + CRIME SCENE SERAQUANT READER
EXCELLENCE IN FORENSIC BIOLOGY BODILY FLUIDS TEST KITS LABORATORY + CRIME SCENE SERAQUANT READER WWW.SERATEC.COM ABOUT US Founded in 1987 as a research spin-off company in the old university town of Goettingen,
IT S ABOUT TIME. IQFISH pharmdx Interpretation Guide THREEHOURSTHIRTYMINUTES. HER2 IQFISH pharmdxtm. TOP2A IQFISH pharmdxtm
I N T E R P R E TAT I O N IQFISH pharmdx Interpretation Guide TM HER2 IQFISH pharmdxtm TOP2A IQFISH pharmdxtm Breast carcinoma (FFPE) stained with HER2 IQFISH pharmdx Breast carcinoma (FFPE) stained with
Pathology and Laboratory
Pathology and Laboratory CPT CPT copyright 2011 American Medical Association. All rights reserved. Fee schedules, relative value units, conversion factors and/or related components are not assigned by
Cell Division Questions. Mitosis and Meiosis
Cell Division Questions Mitosis and Meiosis 1 10 Do not write outside the box 5 Figure 3 shows a pair of chromosomes at the start of meiosis. The letters represent alleles. Figure 3 E E e e F F f f 5 (a)
Biological Evidence: Goals
Biological Evidence: Goals 1. Identify a stain/tissue 2. Identify species of origin - is it human? 3. Individualize it i.e. get a DNA profile? 4. Compare it to a known sample Serology and DNA Profiling
Superior Fluorescent Labeling Dyes Spanning the Full Visible Spectrum...1. Trademarks: HiLyte Fluor (AnaSpec, Inc.)
Table of Contents Fluor TM Labeling Dyes Superior Fluorescent Labeling Dyes Spanning the Full Visible Spectrum....1 Fluor TM 405 Dye, an Excellent Alternative to Alexa Fluor 405 & DyLight 405....2 Fluor
*Corresponding author:
LIPID DROPLETS HYPERTROPHY: A CRUCIAL DETERMINING FACTOR IN INSULIN REGULATION BY ADIPOCYTES 1 Bahram Sanjabi #, 2,3 Monireh Dashty #, 4 Behiye. Özcan, 5 Vishtaseb Akbarkhanzadeh, 3 Mehran Rahimi, 7,8
Strategic Approaches to Sexual Assault Kit Evidence. An Action Research Project
Strategic Approaches to Sexual Assault Kit Evidence An Action Research Project 1 Introduction Purpose: To walk you through the solicitation and respond to your questions. This is not a seminar on sexual
1. The diagram shows four stages in mitosis. Only one pair of homologous chromosomes is shown. A B C D ... (1) ... (1)
1. The diagram shows four stages in mitosis. Only one pair of homologous chromosomes is shown. X A B C D (a) Place stages A, B, C and D in the correct order.... (b) Name the structures labelled X.... Describe
Instant Quality FISH. The name says it all.
COMPANION DIAGNOSTICS Instant Quality FISH Instant Quality FISH. The name says it all. IQ: Instant Quality every time. Breast carcinoma stained with : Triple filter showing Blue DAPI colors nuclei, FITC
Lipid Droplets Fluorescence Assay Kit
Lipid Droplets Fluorescence Assay Kit Item No. 500001 www.caymanchem.com Customer Service 800.364.9897 Technical Support 888.526.5351 1180 E. Ellsworth Rd Ann Arbor, MI USA TABLE OF CONTENTS GENERAL INFORMATION
Three Hours Thirty Minutes
INTERPRETATION HER2 IQFISH pharmdx TM Interpretation Guide Three Hours Thirty Minutes it s about time Breast carcinoma (FFPE) stained with HER2 IQFISH pharmdx Gastric cancer (FFPE) stained with HER2 IQFISH
MEMORIAL HOSPITAL SEXUAL ASSAULT MEDICAL/FORENSIC RECORD
MEMORIAL HOSPITAL SEXUAL ASSAULT MEDICAL/FORENSIC RECORD Name and Location of Examining Facility: Address: Date of Admission: Time: Name of Patient: Date of Birth: Address: Brought in by: Others Present
HIGH SCHOOL SCIENCE CURRICULUM FORENSIC SCIENCE
944 STATE ROUTE 17K MONTGOMERY, NY 12549 Telephone Number: (845) 457-2400 ext. 8121 Fax Number: (845) 457-4254 HIGH SCHOOL SCIENCE CURRICULUM FORENSIC SCIENCE JULY 2015 Approved by the Board of Education
Use Tomorrow s Technology Today
Use Tomorrow s Technology Today Company Profile (MES) is a leading edge company specializing in rapid, automated semen analysis for both the human and the veterinary markets. The Company s comprehensive
Acute SART Exams: Maximizing Evidence Recovery and Victim Care. Marilyn Kaufhold, MD Chadwick Center for Children and Families
Acute SART Exams: Maximizing Evidence Recovery and Victim Care Marilyn Kaufhold, MD Chadwick Center for Children and Families 858-966-5980 Learning Objectives The participant will be able to List the usual
Development of a thiol-reactive fluorescent probe for the identification of human spermatozoa
Boston University OpenBU Theses & Dissertations http://open.bu.edu Boston University Theses & Dissertations 2017 Development of a thiol-reactive fluorescent probe for the identification of human spermatozoa
Breast Cancer Interpretation Guide
Breast Cancer Interpretation Guide UCT D O R P NEW ERBB2/ C E P S ht e ZytoLig lor Prob o C l a u 2D D17S12 ng to the i d r o c c a ting for re-tes idelines 2013 ASCO Gu Breast Cancer Interpretation Guide
August 2017 Changes. Reproductive Laboratory Checklist. CAP Accreditation Program
August 2017 Changes Reproductive Laboratory Checklist CAP Accreditation Program College of American Pathologists 325 Waukegan Road Northfield, IL 60093-2750 www.cap.org 08.21.2017 Disclaimer and Copyright
Vaginal swab specimen processing methods influence performance of rapid semen detection tests: a cautionary tale
Contraception 82 (2010) 291 295 Original research article Vaginal swab specimen processing methods influence performance of rapid semen detection tests: a cautionary tale Marcia M. Hobbs,a, Markus J. Steiner
Unit 7: Blood/ Serology
Unit 7: Blood/ Serology Forensic Serology the examination and analysis of body fluids including: saliva semen urine Blood 1950-1980 s, forensic serology = most important part of lab procedures (until DNA).
It is essential to have the victim(s) examined by a medical
Sexual Offenses Chapter 7 It is essential to have the victim(s) examined by a medical professional as soon as possible after the assault and before the affected areas (pubic area, vagina, rectum, etc.)
-SQA-SCOTTISH QUALIFICATIONS AUTHORITY HIGHER NATIONAL UNIT SPECIFICATION GENERAL INFORMATION JULY
-SQA-SCOTTISH QUALIFICATIONS AUTHORITY HIGHER NATIONAL UNIT SPECIFICATION GENERAL INFORMATION -Unit number- 7670017 -Unit title- -Superclass category- -Date of publication- (month and year) -Originating
ph and Reduction Dual Responsive Polyurethane Triblock Copolymers for Efficient Intracellular Drug Delivery
Supporting Information ph and Reduction Dual Responsive Polyurethane Triblock Copolymers for Efficient Intracellular Drug Delivery Shuangjiang Yu a, Chaoliang He* a, Jianxun Ding a,b, Yilong Cheng a,b,
Lecture 1: Characteristics & Testing Of Blood
Lecture 1: Characteristics & Testing Of Blood Blood Physiology: Composition of Blood Plasma (55% of the mix) is the fluid portion of the blood. Cells (45% of the mix ) Red Blood Cells are responsible for
Workflow. Connecting the Pieces For Total Patient Care
Workflow Connecting the Pieces For Total Patient Care Biocare provides a full line of IHC and molecular pathology products for cancer and infectious disease diagnosis. From a full range of equipment: including
Identification of human semenogelin in membrane strip test as an alternative method for the detection of semen
Forensic Science International 169 (2007) 27 31 www.elsevier.com/locate/forsciint Identification of human semenogelin in membrane strip test as an alternative method for the detection of semen B.C.M. Pang
THE WINSTON CHURCHILL MEMORIAL TRUST OF AUSTRALIA
THE WINSTON CHURCHILL MEMORIAL TRUST OF AUSTRALIA Report by Kirsty Wright 2003 Churchill Fellow To investigate improved methods of DNA profiling for use in forensic casework and disaster victim identification.
Medical Forensic Response in Minnesota How Are We Doing? Frequently Asked Questions About Sexual Assault Examinations in Minnesota
Medical Forensic Response in Minnesota How Are We Doing? Frequently Asked Questions About Sexual Assault Examinations in Minnesota 1) Why did MNCASA conduct a survey of the medical forensic response in
Mouse sperm extraction:
Mouse sperm extraction: This method of extraction is used for acrosome reaction assays, immunocytochemistry and biochemical assays. Collect two cauda epidydimus from one male, cut them 5 times and place
year 7 REVISION Spring Assessment - Biology
year 7 REVISION Spring Assessment - Biology year 7 REVISION Microscope and Cells THE MICROSCOPE Used to view things too small for the naked eye. Consists of lenses of different magnification to make things
Blood Smears Only 3 February Sample Preparation and Quality Control
NEW YORK STATE Parasitology Proficiency Testing Program Blood Smears Only 3 February 2015 The purpose of the New York State Proficiency Testing Program in the category of Parasitology - Blood Smears Only
What does your body do to protect itself from invading microbes?
46 Disease Fighters i n v e s t i g at i o n What does your body do to protect itself from invading microbes? Even before an organism can enter your body, your skin provides a protective barrier. But foreign
Clinical and Anatomic Pathology Test Volume by Specialty and Subspecialty Among High- Complexity CLIA-Certified Laboratories in 2011
Clinical and Anatomic Pathology Test Volume by Specialty and Subspecialty Among High- Complexity CLIA-Certified Laboratories in 2011 Frank H. Wians, Jr., PhD, MT(ASCP), 1 Gary W. Gill, BA, CT(ASCP) 2 It
EXPOSURE (HIV/HEPATITIS) BLOOD & BODY FLUIDS
Page(s): 1 of 11 PURPOSE To set a standardized procedure to ensure that employees are evaluated in a consistent and timely manner.. POLICY A. The treatment of Team Member exposure to bloodborne pathogens
2008 CAP TODAY Q & A
2008 CAP TODAY Q & A Q. How often should we document hematology competencies to ensure consistency of morphologic observations for manual differentials and fluids? Should we do this every six months or
Application of Pressure Cycling Technology (PCT) in Differential Extraction
Application of Pressure Cycling Technology (PCT) in Differential Extraction Deepthi Nori, MFS; Dr. Bruce R. McCord, PhD Department of Chemistry International Forensic Research Institute Florida International
Pacing Guide for 7-12 Curriculum
Pacing Guide for 7-12 Curriculum Course Title: Science Length of Course: 36 weeks Week Number Week 1 Chapter/ Topic Introduction/ Syllabus Lab Safety procedures, review and test COS Biology COS 1. Select
Laboratory Training. Summer Internship 2015 ACRM
Laboratory Training Summer Internship 2015 ACRM Pipetting Volumes: 1000µL, 100µL, 10µL 15 repeats each Acceptable variation 5% Method Pipette distilled H20 onto a balance and record the weight Microbeads
Cold Case Investigation
1 Cold Case Investigation Expanded Course Outline and Hourly Distribution For Two-Day, 16 Hour Course INSTRUCTOR: Michael Gaynor Third Degree Communications, Inc. Learners: Approximately 20 In-Service
Computerized semen analysis. Product features. Basic system
AndroVision - more than CASA AndroVision CASA system with Zeiss AxioScope optics and automated ScanStage Computerized semen analysis AndroVision is a highly precise CASA* system for standardised, interactive
Explain the laboratory diagnosis of Rabies?
Explain the laboratory diagnosis of Rabies? The standard test for rabies testing is dfa. This test has been thoroughly evaluated for more than 40 years, and is recognized as the most rapid and reliable
Physician Office Laboratory Tests
Important Change Effective March 1, 2018 Physician Office Laboratory Tests Molina Healthcare of Michigan has updated its list of payable laboratory tests that may be performed in a physician s office.
Intro to the Evidence Kit
PHYSICAL EVIDENCE Intro to the Evidence Kit Cheryl Ann Graf ARNP, MSN, MBA Something that shows or proves, or that gives reason for believing; proof or indication (Webster s New World Dictionary;1995)
Microscope Requirements
SEMEN EVALUATION EQUIPMENT Microscope Requirements Good quality lenses Phase-contrast preferred for % progressive motility evaluations Objectives 10X, 20X*, 40X*, 100X, minimum Heated stage preferred *Preferably
1. Police investigation of sex crimes. (1) Serious injury or death of victim. (3) Arrest of suspect. 2. Response to sexual assault report.
8103 SEXUAL ASSAULT INVESTIGATION PROCEDURES A. Policy The crime of sexual assault is one of the most violent crimes the Police Department will be called upon to investigate. It shall be our intent that
National Outreach Priorities & Agenda
Accreditation 2017-2018 National Outreach Priorities & Agenda ASCLD supports mandatory accreditation of forensic science service providers (FSSPs). ASCLD believes the accreditation of all FSSPs is an essential
RayBio Annexin V-FITC Apoptosis Detection Kit
RayBio Annexin V-FITC Apoptosis Detection Kit User Manual Version 1.0 May 25, 2014 (Cat#: 68FT-AnnV-S) RayBiotech, Inc. We Provide You With Excellent Support And Service Tel:(Toll Free)1-888-494-8555 or
Cells and viruses. Human isolates (A/Kawasaki/173/01 [H1N1], A/Yokohama/2057/03 [H3N2],
![Cells and viruses. Human isolates (A/Kawasaki/173/01 [H1N1], A/Yokohama/2057/03 [H3N2],](/thumbs/86/93801604.jpg "Cells and viruses. Human isolates (A/Kawasaki/173/01 [H1N1], A/Yokohama/2057/03 [H3N2],")
Supplementary information Methods Cells and viruses. Human isolates (A/Kawasaki/173/01 [H1N1], A/Yokohama/2057/03 [H3N2], and A/Hong Kong/213/03 [H5N1]) were grown in Madin-Darby canine kidney (MDCK) cells
Microscopic Examination of Urine
Download http://www.vetlab.com/kova.htm Definition of urine sediment: all solid materials suspended in the urine - a semiquantative evaluation of the urine sediment Significance of formed elements in the
FOR OPTIMAL GUT HEALTH KEMIN.COM/GUTHEALTH
FOR OPTIMAL GUT HEALTH KEMIN.COM/GUTHEALTH ALETA A SOURCE OF 1,3-BETA GLUCANS Aleta is highly bioavailable, offering a concentration greater than 5% of 1,3-beta glucans. Aleta provides a consistent response
Trace Evidence Service
Trace Evidence Service Trace Evidence Fibres Glass, Paint and other Particulates Hairs Toolmarks Right People. Delivering Results. Experienced scientists delivering forensic effectiveness, unquestionable
Development and Testing of a Mass Spectrometry Based Assay for the Identification of Biological Stains. Kevin Legg
Development and Testing of a Mass Spectrometry Based Assay for the Identification of Biological Stains Kevin Legg This project was supported by DNA R&D Grants 2006-DN-BX-K001, 2009-DN-BX-K165, 2011-CD-BX-0205,
For the rapid, sensitive and accurate measurement of apoptosis in various samples.
ab14082 500X Annexin V-FITC Apoptosis Detection Reagent Instructions for Use For the rapid, sensitive and accurate measurement of apoptosis in various samples. This product is for research use only and
RECOMMENDATIONS OF FORENSIC SCIENCE COMMITTEE
To promote the development of forensic science into a mature field of multidisciplinary research and practice, founded on the systematic collection and analysis of relevant data, Congress should establish
ANDROVISION - MORE THAN CASA
ANDROVISION - MORE THAN CASA AndroVision CASA system with Zeiss AxioScope optics and automated ScanStage Computerized semen analysis AndroVision is a highly precise CASA* system for standardised, interactive
Chapter 17 Worksheet Code It
Class: Date: Chapter 17 Worksheet 3 2 1 Code It True/False Indicate whether the statement is true or false. 1. CPT laboratory codes include collection of the specimen. 2. A dipstick is a small piece of
Medicaid Family Planning Waiver Services CPT Codes and ICD-10 Diagnosis Codes
CPT Code Description of Covered Codes Evaluation and Management 99384FP 99385FP Family planning new visit 99386FP 99394FP 99395FP Family planning established visit 99396FP 99401FP HIV counseling (pre-test)
A. Macroscopic and Physical tests:- 1. Volume 2. Colour 3. Consistency and cloudiness 4. Osmotic pressure 5. Specific Gravity 6. Electro Conductivity
EXAMINATION AND EVALUATION OF SEMEN. Evaluation of Semen. A. Macroscopic and Physical tests:- 1. Volume 2. Colour 3. Consistency and cloudiness 4. Osmotic pressure 5. Specific Gravity 6. Electro Conductivity
Deciphering the biology that drives response to immunotherapy
Deciphering the biology that drives response to immunotherapy Phenoptics TM Quantitative Pathology Platform Trent Norris, Field Application Scientist September 15, 2016 HUMAN HEALTH ENVIRONMENTAL HEALTH
Effective June 1, 2018
EMPIRE PHYSICIAN OFFICE LAB (POL) LIST Approved Laboratory Tests for Physician Offices Reimbursement is only available if the benefit is covered under the member's contract. Medical Policy edits will apply.
National Body Fluid Guidance Notes
1) Blood 1a. Examination for Blood National Body Fluid Guidance Notes Searching for blood typically involves the combination of careful visual inspection aided by oblique lighting, low power microscopy,
Vision Hema. Additional modules
Vision Hema Additional modules Vision Hema RTC Digital morphology of reticulocytes Innovative solution for the study of reticulocytes Reticulocytes Reticulocytes young erythrocytes that appear in the bone
COUNTY CRIME LAB: HIGH QUALITY TEST RESULTS, CHRONICALLY DELAYED
Contact: Olga Jones Foreperson (925) 957-5879 COUNTY CRIME LAB: HIGH QUALITY TEST RESULTS, CHRONICALLY DELAYED Severe Understaffing Compromises County Lab s Criminalistics Section Performance SUMMARY Sophisticated
Optimizing Intracellular Flow Cytometry
Optimizing Intracellular Flow Cytometry Detection of Cytokines, Transcription Factors, and Phosphoprotein by Flow Cytometry Presented by Erika O Donnell, PhD, BD Biosciences 23-14876-00 Outline Basic principles
Instructions for Use. APO-AB Annexin V-Biotin Apoptosis Detection Kit 100 tests
3URGXFW,QIRUPDWLRQ Sigma TACS Annexin V Apoptosis Detection Kits Instructions for Use APO-AB Annexin V-Biotin Apoptosis Detection Kit 100 tests For Research Use Only. Not for use in diagnostic procedures.
Forensic Science. Forensic Serology
Forensic Science Forensic Serology Composition of Blood Blood is a complex mixture of cells, enzymes, proteins, and inorganic substances Main Components: Erythrocytes = red blood cells (RBCs) Leukocytes
CANCER. Clinical Validation of Breast Cancer Predictive Markers
Clinical Validation of Breast Cancer Predictive Markers David Hicks, MD Loralee McMahon, MS, HTL(ASCP) CANCER The human body is composed of billions of highly regulated cells Cancer cells no longer respond
Anti-ceramide Antibody Prevents the Radiation GI Syndrome in Mice
Anti-ceramide Antibody Prevents the Radiation GI Syndrome in Mice Jimmy A. Rotolo 1, Branka Stancevic 1, Jianjun Zhang 1, Guoqiang Hua 1, John Fuller 1, Xianglei Yin 1, Adriana Haimovitz-Friedman 2, Kisu
Forensic Science (One Credit).
130.295. Forensic Science (One Credit). (a) General requirements. The course is recommended for students in Grades 11-12. Prerequisites: Biology and Chemistry. Recommended prerequisites: Principles of
Blood. Chapter 10. Kendall/Hunt Publishing Company 1
Blood Students will learn: That an antibody and an antigen of different types will agglutinate, or clump, when mixed together. That the significance of the evidence depends on a characteristic s relative
Medical Care for Obese Patients
Medical Care for Obese Patients WIN Weight-control Information Network U.S. DEPARTMENT OF HEALTH AND HUMAN SERVICES Approximately 30 percent of adults in the United States are obese, up from 15 percent
Topics: Mitosis, Meiosis, Mendel s Genetics, Lab Skills
Topics: Mitosis, Meiosis, Mendel s Genetics, Lab Skills Asexual Reproduction making genetically identical offspring from a single parent. o Regeneration organisms ability to loose body parts Ex. Starfish,
Epithelial interleukin-25 is a key mediator in Th2-high, corticosteroid-responsive
Online Data Supplement: Epithelial interleukin-25 is a key mediator in Th2-high, corticosteroid-responsive asthma Dan Cheng, Zheng Xue, Lingling Yi, Huimin Shi, Kan Zhang, Xiaorong Huo, Luke R. Bonser,
Oncology Genetics: Cytogenetics and FISH 17/09/2014
Oncology Genetics: Cytogenetics and FISH 17/09/2014 Chris Wragg Head of Oncology Genomics, BGL BGL Bristol Genetics Laboratory (BGL) CPA accredited Genetics laboratory serving a core population of 4-5million
SEMEN ANALYSIS DISPOSABLES AND COMPONENTS MORE THAN JUST BASIC SEMEN ANALYSIS
SEMEN ANALYSIS DISPOSABLES AND COMPONENTS MORE THAN JUST BASIC SEMEN ANALYSIS mot MOTILITY AND CONCENTRATION SCA Leja slides Disposable counting chambers for semen analysis, optimised for SCA. 10 micron
ATTORNEY GENERAL LAW ENFORCEMENT DIRECTIVE NEW JERSEY FORENSIC SCIENCE COMMISSION
ATTORNEY GENERAL LAW ENFORCEMENT DIRECTIVE 2006-1 NEW JERSEY FORENSIC SCIENCE COMMISSION WHEREAS, it is the public policy of New Jersey, in accordance with N.J.S.A. 52:17B-98, to encourage cooperation
Bio10 Lab 2: Cells. Using your text and the cell models and posters in the lab, sketch an animal cell and a plant cell on the group results sheet.
Bio10 Lab 2: Cells Cells are the smallest living things and all living things are composed of cells. They are able to perform all necessary metabolic functions as well as specialized tasks such as moving,