Supplementary Information
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1 Supplementary Information Table S1. Donor and sample information for blood samples used in the microarray and gene expression studies. Sample ID Age (Years) Diagnosis Smoking Classification DNA Methylation Gene Expression ER AAA No AAA ER AAA Yes AAA - ER AAA Yes AAA ER AAA No AAA - ER AAA Yes AAA 5.80 ER AAA Yes AAA - ER AAA Yes AAA 6.50 ER AAA Yes AAA 7.30 ER VV No Control ER VV No Control 8.90 ER VV Yes Control 8.60 ER VI No Control ER VV Yes Control ER VV No Control - ER AAA No AAA - ER AAA No AAA - ER VV No Control 8.80 ER ESRD No Control 6.30 ER AAA No AAA ER VTE Yes Control 3.20 ER Unknown Yes Control 9.10 ER DVT Yes Control 8.40 ER DVT Yes Control ER VV Yes Control 8.50 ER VV No Control 8.50 ER AAA Yes AAA 8.50 ER Unknown Yes Control 8.70 ER AAA Yes AAA 8.50 ER AAA Yes AAA 8.00 ER CD Yes Control 9.10 ER AAA Yes AAA 8.60 ER CD No Control 9.30 ER AVM No Control ER CD No Control 9.00 ER ESRD Yes Control 8.50 ER AAA No AAA ER VV Yes Control ER CD No Control 8.20 ER AAA No AAA 5.40 ER AAA No AAA 8.30 ER AAA Yes AAA 8.80 ER AAA No AAA RIN
2 S2 Sample ID Age (Years) Table S1. Cont. Diagnosis Smoking Classification DNA Methylation Gene Expression ER AAA No AAA ER AAA No AAA - - ER AAA Yes AAA - - ER CD No Control - - ER CABG No Control - - ER AAA No AAA - - ER CD Yes Control - - ER AAA No AAA - - ER AAA Yes AAA - - ER AAA No AAA - - ER AAA No AAA - - ER AAA Yes AAA - - ER AAA Yes AAA - - ER AAA No AAA - - AAA, abdominal aortic aneurysm; AVM, arteriovenous malformation; CABG, coronary artery bypass graft; CD, carotid disease; DVT, deep vein thrombosis; ESRD, end stage renal disease; RIN, RNA Integrity Number obtained from Agilent BioAnalyzer; VI, venous insufficiency; VTE, venous thromboembolism; VV, varicose veins. All controls were without aneurysmal disease at the time of recruitment. See Table 1 for summary of sample groups used for the analyses. This table only includes samples that passed quality control filters and were used in the final analysis for both the DNA methylation and gene expression studies. RIN
3 S3 Table S2. Annotation for individual CpGs contained in the CpGIs that contributed to the principal components. chr4: cg NA NA Island chr cg NA NA S_Shore chr cg NA NA S_Shelf chr chr6: cg SERPINB9 3'-UTR N_Shelf chr cg SERPINB9 Body N_Shore chr cg SERPINB9 Body Island chr cg SERPINB9 Body Island chr cg SERPINB9 Body Island chr cg SERPINB9 Body Island chr cg SERPINB9 Body Island chr cg SERPINB9 Body S_Shore chr cg SERPINB9 Body S_Shelf chr chr6: cg NFYA Body N_Shelf chr cg NFYA; LOC '-UTR; TSS1500 N_Shore chr cg NFYA; LOC '-UTR; TSS1500 N_Shore chr cg NFYA; LOC '-UTR; TSS1500 Island chr cg NFYA; LOC '-UTR; TSS200 Island chr cg NFYA; LOC '-UTR; TSS200 Island chr cg NFYA; LOC '-UTR; TSS200 Island chr cg NFYA; LOC '-UTR; TSS200 Island chr cg NFYA; LOC '-UTR; TSS200 Island chr cg NFYA; LOC '-UTR; TSS200 Island chr cg NFYA; LOC '-UTR; Body Island chr cg NFYA; LOC '-UTR; Body Island chr cg LOC Body S_Shore chr cg LOC Body S_Shelf chr
4 S4 Table S2. Cont. chr6: cg KIF25 Body N_Shelf chr cg KIF25 Body N_Shelf chr cg KIF25 Body N_Shore chr cg KIF25 Body Island chr cg KIF25 Body Island chr cg KIF25 Body Island chr cg KIF25 Body S_Shore chr cg KIF25 Body S_Shore chr cg KIF25 Body S_Shelf chr chr9: cg LHX6 Body N_Shore chr cg LHX6 Body Island chr cg LHX6 Body Island chr cg LHX6 Body Island chr cg LHX6 Body Island chr cg LHX6 Body Island chr cg LHX6 Body Island chr cg LHX6 Body Island chr cg LHX6 Body Island chr cg LHX6 Body Island chr cg LHX6 Body Island chr cg LHX6 TSS200 Island chr cg LHX6 TSS1500 S_Shore chr cg LHX6 TSS1500 S_Shore chr chr11: cg PKP3 Body Island chr cg PKP3 Body Island chr cg PKP3 Body Island chr
5 S5 Table S2. Cont. chr11: cg KLHL35 Body N_Shore chr cg KLHL35 Body Island chr cg KLHL35 Body Island chr cg KLHL35 Body Island chr chr17: cg RPH3AL Body N_Shelf chr cg RPH3AL Body N_Shelf chr cg RPH3AL Body N_Shelf chr cg RPH3AL Body N_Shore chr cg RPH3AL Body N_Shore chr cg RPH3AL Body Island chr cg RPH3AL Body Island chr cg RPH3AL Body Island chr cg RPH3AL Body Island chr cg RPH3AL Body S_Shore chr cg RPH3AL Body S_Shore chr chr17: cg NA NA Island chr cg NA NA Island chr chr19: cg HCN2 Body Island chr cg HCN2 Body Island chr cg HCN2 Body Island chr cg HCN2 Body Island chr chr19: cg CNN2 Body N_Shore chr cg CNN2 Body Island chr cg CNN2 Body Island chr cg CNN2 Body S_Shore chr
6 S6 Table S2. Cont. chr19: cg NA NA N_Shore chr cg LMTK3 Body Island chr cg LMTK3 Body Island chr cg LMTK3 Body Island chr cg LMTK3 Body Island chr cg LMTK3 Body S_Shelf chr chr20: cg NECAB3; C20orf134 Body; TSS1500 N_Shore chr cg NECAB3; C20orf134 Body; TSS1500 N_Shore chr cg NECAB3; C20orf134 Body; TSS200 N_Shore chr cg NECAB3; C20orf134 Body; 1st Exon N_Shore chr cg NECAB3; C20orf134 Body; 1st Exon 5'-UTR Island chr cg NECAB3; C20orf134 Body; 1st Exon Island chr cg NECAB3; C20orf134 Body; 1st Exon Island chr cg NECAB3; C20orf134 Body; 1st Exon 3'-UTR S_Shore chr chr21: cg DSCR3 Body N_Shelf chr cg DSCR3 Body N_Shore chr cg DSCR3 Body N_Shore chr cg DSCR3 Body Island chr cg DSCR3 Body Island chr cg DSCR3 Body Island chr cg DSCR3 Body S_Shore chr cg DSCR3 Body S_Shelf chr chrx: cg PABPC1L2A 1st Exon 3'-UTR N_Shore chrx cg PABPC1L2A 1st Exon 5'-UTR S_Shore chrx Annotations from IlluminaHumanMethylation450kanno.ilmn12.hg19 [48]. NA, not applicable, since the CpGI was not in any gene. UTR: Untranslated Regions.
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