Induction of Drought Stress Tolerance by Paclobutrazol and Abscisic Acid in Gingelly (Sesamum indicum L.)

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1 Global Journal of Molecular Sciences 4 (2): 49-55, 29 ISSN IDOSI Publications, 29 Induction of Drought Stress Tolerance by Paclobutrazol and Abscisic Acid in Gingelly (Sesamum indicum L.) 1 1 2,3 R. Somasundaram, Cheruth Abdul Jaleel, M.M. Azooz, Sindhu S. Abraham, M. Gomathinayagam and R. Panneerselvam 1 Stress Physiology Lab, Department of Botany, Annamalai University, Annamalainagar 68 2, Tamilnadu, India 2 Department of Botany, Faculty of Science, South Valley University, Qena, Egypt 3 Department of Biology, Faculty of Science, King Faisal University, P.O. Box: 38, Al-Hassa 31982, Saudi Arabia Abstract: For the past several years, several techniques of physiology have been applied to overcome the water deficit and drought stress in field crops. However little information is gained on the response to PBZ and ABA treatments under drought stress and their ameliorative actions on Sesamum. So a study was carried out to understand the effect of PBZ and ABA on drought stress amelioration in Sesamum indicum L. The main aspects studied were the non-enzymatic antioxidant changes in different parts of treated, drought stressed as well as control plants. The non-enzymatic antioxidants (ascorbic acid and tocopherol) were extracted and estimated. The non-enzymatic antioxidant molecules like ascorbate and -tocopherol showed significant increase under drought condition in Sesamum indicum. PBZ caused significant enhancement in these antioxidant enzymes under drought stress and also in well-watered conditions. It is not so with ABA treatment. ABA slightly reduced the non-enzymatic antioxidant contents. From the results, it can be concluded that, these growth regulators are better suited as stress ameliorating agents in gingelly. Key words: Non-enzymatic antioxidants Ascorbic acid Tocopherol Drought stress Sesamum indicum INTRODUCTION physiological responses of plant to water deficits generally vary with the severity as well as the duration of Soil water availability represents a major water stress [6]. environmental constraint under Mediterranean Water stress is considered to be a moderate loss of conditions and predictions suggest that the water, which leads to stomatal closure and limitation of decline in total rainfall in the Mediterranean area will gas exchange. Desiccation is a much more extensive loss be drastic. Under such conditions, it is likely that plants of water which can potentially lead to gross disruption of will experience increasing water deficit stress in their metabolism and cell structure and eventually to the natural communities [1-4]. Water stress tolerance is seen cessation of enzyme catalyzed reaction [7,8]. Water stress in almost all plant species but its extent varies from is characterized by reduction of water content, turgor, species to species [2-3]. A better understanding of the total water potential, wilting, closure of stomata and physiological strategy adopted by a drought resistant decrease in cell enlargement and growth. Severe water variety to cope with water deficit requires through study stress may result in arrest of photosynthesis, disturbance of the relationship between water use efficiency and of metabolism and finally dying [6-9]. transpiration. In crops like wheat, the detrimental effects Tolerance to abiotic stresses is very complex, of water deficits on the harvest index also minimizes the due to the complexity of interactions between stress impact of the water limitation on crop productivity and factor and various molecular, biochemical and increase the efficiency of water use [5]. The numerous physiological phenomena affecting plant growth and Corresponding Author: Dr. R. Somasundaram, Stress Physiology Lab, Department of Botany, Annamalai University, Annamalainagar 68 2, Tamilnadu, India 49

2 development [1-12]. High yield potential is the target of between 6-75 per cent. The experimental part of this most crop breeding, not superior drought resistance and work was carried out in Botanical Garden and Stress in many cases high yield potential can contribute to yield Physiology Lab, Department of Botany, Annamalai in moderate stress environment [7-9]. University, Tamil Nadu. The methodologies adopted are PBZ (2RS, 3RS)-1-(4-chlorophenyl)-4,4-dimethyl-2- described below. (1H-1,2,4-trizol-1-yl)-pentan-3-ol] is a triazolic group of The plants were raised in Botanical Garden, during fungicide which have PGR properties. The growth the months of February May, 26. The seeds were regulating properties of PBZ are mediated by changes in surface sterilized with.2% Mercuric chloride solution for the balance of important plant hormones including the five minutes with frequent shaking and thoroughly Gibberellins, ABA and cytokinins [13-15]. The application washed with tap water. The experiments were carried out of PBZ have no risk to human health and showed in polythene bags (27x16 cm). The pots were filled with increased yield of crops. PBZ has been proved as an 3 kg uniform soil mixture containing red soil: sand: farm agent in stress amelioration in medicinal plants [16-19]. yard manure (FYM) in 1:1:1 ratio. The experiment was laid In spite of the plants growth regulating properties, out in a Completely Randomized Block Design (CRBD). it is reported that PBZ have some negative side effects In the preliminary experiments, 2, 5, 1, 15 and on soil microorganisms. Anyhow, there are reports on -1 2 mg L paclobutrazol and 5, 1 and 15 ìm ABA were microorganisms, which are capable of PBZ degradation used for treatment to determine the optimum on soil. -1 concentration. Among the treatments, 5 mg L Paclobutrazol has been proved as an agent in stress paclobutrazol and 1 ìm ABA concentration increased amelioration in medicinal plants. Paclobutrazol increased the growth and dry weight significantly and higher the diameter and length of fibrous roots and enhanced concentration slightly decreased the growth and dry the lateral root formation [14-16]. Gingelly (Sesamum weight when compared to drought stressed plants. In the indicum L.) is a member of the Pedaliaceae family. lower concentrations, there was no change in weight and Gingelly is one of the ancient oil seed crop cultivated for -1 growth. Hence 5 mg L paclobutrazol concentration was its superior quality oil and seed, hence it is regarded as used to study the effect of paclobutrazol and 1 ìm ABA Queen of the oil seeds. This species is native to Africa. on the drought stress amelioration of Sesamum indicum. It is cultivated extensively in USA, India, Burma, Drought Treatment intervals were from 3 DAS 2, 4 Indo-china and Japan. India is the largest producer of and 6 days interval drought (DID). The treatments were sesame in the world. Tamil Nadu is one of the major given as foliar spray for ABA and soil drenching for PBZ sesame growing state in India. Sesame is used in on 32, 34 and 36 days after sowing (DAS). traditional systems of medicine for curing many diseases. The plants were taken randomly on 33 (2 DID), It has a good composition of amino acids, protein, niacin, 35 (4 DID) and 37 (6 DID) DAS and separated into roots, folic acid, vitamin E, calcium and Phosphorus. stems and leaves and used for determining antioxidant The objectives of the present study were to potentials. understand the effect of paclobutrazol and ABA in drought stress amelioration in Sesamum indicum L. Antioxidants through their effects on the plant s non-enzymatic Ascorbic Acid: Ascorbic acid content was assayed as antioxidant potential under drought stress conditions. described by Omaye et al. [2]. MATERIALS AND METHODS Extraction: One gram of fresh material was ground in a pestle and mortar with 5 ml of 1 per cent TCA, the extract The seeds of Sesamum indicum L. were obtained was centrifuged at 35 rpm for 2 minutes. The pellet was from Department of Agronomy, Faculty of Agriculture, re-extracted twice with 1 percent TCA and supernatant Annamalai University. The triazole compound was made to 1 ml and used for estimation. paclobutrazol was obtained from Syngenta, India Ltd., Mumbai. ABA was purchased from Himedia India Ltd., Estimation: To.5 ml of extract, 1 ml of DTC reagent Mumbai. (2,4-Dinitrophenyl hydrazine-thiourea-cuso 4 reagent) During the study, average temperature was 32/26 C was added and mixed thoroughly. The tubes were (maximum/minimum) and relative humidity (RH) varied incubated at 37 C for 3 hours and to this.75 ml of 5

3 Root Leaf Stem Fig. 1: Individual and combined effects of drought, PBZ, ABA on ascorbic acid contents o f Sesamum indicum ice cold 65 per cent H2SO 4 was added. The tubes at 52 nm. The -tocopherol content was calculated were then allowed to stand at 3 C for 3 minutes. The using a standard graph made with known amount of resulting colour was read at 52 nm in spectrophotometer -tocopherol. (U-21-Hitachi). The ascorbic acid content was determined using a-standard curve prepared with ascorbic Statistical Analysis: Each treatment was analysed with at acid and the results were expressed in milligrams per gram least seven replicates and a standard deviation (SD) was dry weight. calculated and data are expressed in mean ± SD of seven replicates. RESULTS -Tocopherol: -Tocopherol activity was assayed as described by Backer et al. [21]. Ascorbic Acid (Fig. 1) Root: In the roots the ascorbic acid content was increased Extraction: Five hundred milligrams of fresh tissue was with age in paclobutrazol treated, drought stressed and homogenized with 1 ml of a mixture of petroleum ether control plants. ABA decreased the ascorbic acid content and ethanol (2:1.6 v/v) and the extract was centrifuged at in roots at all stages of growth and it was 6.99 per cent 1, rpm for 2 minutes and the supernatant was used and per cent over control in well watered and for estimation of -tocopherol. stressed respectively on 6 DID. Estimation: To one ml of extract,.2 ml of 2 per cent 2,2-dipyridyl in ethanol was added and mixed thoroughly and kept in dark for 5 minutes. The resulting red colour was diluted with 4 ml of distilled water and mixed well. The resulting colour in the aqueous layer was measured Stem: The ascorbic acid content of the stem of Sesamum indicum increased with age in the treated and control plants. Paclobutrazol and drought treatments increased the ascorbic acid content in the stem at all stages of growth and it was per cent and per cent over 51

4 Root Stem 4 Leaf 3 2 Fig. 2: Individual and combined effects of drought, PBZ, ABA on a-tocopherol contents o f Sesamum indicum control respectively on 6 DID. ABA resulted a significant Stem: The -tocopherol content of the stem of Sesamum reduction (56.16 per cent over control) in ascorbic acid indicum increased with age in the treated and control content in stem of Sesamum indicum at 6 DID. plants. Paclobutrazol and drought treatments increased the -tocopherol content in the stem at all stages of Leaf: The ascorbic acid content of the leaves of growth and it was per cent and 39.2 per cent over Sesamum indicum increased with age in the treated control respectively on 6 DID. ABA resulted a significant and control plants. Paclobutrazol and drought reduction in -tocopherol content in stem of Sesamum treatments increased the ascorbic acid content in the indicum at all sampling days. leaves at all stages of growth and it was per cent and per cent over control respectively on 6 DID. Leaf: In the leaves the -tocopherol content was ABA resulted a significant reduction in ascorbic acid increased with age in paclobutrazol treated, drought content in leaves of Sesamum indicum at all stressed and control plants. ABA decreased the sampling days. -tocopherol content in leaves at all stages of growth and it was per cent and per cent over control in -Tocopherol (Fig. 2) well watered and stressed respectively on 6 DID. Root: -tocopherol of the drought stressed plant roots significantly increased when compared to DISCUSSION control plants. The extent of increase was per cent over control on 6 DID. Paclobutrazol caused Ascorbic Acid: The ascorbic acid content was increased an enhancement in -tocopherol content of roots with age in paclobutrazol treated drought stressed and under drought as well as well-watered conditions. ABA control plants. ABA decreased the ascorbic acid content was an inhibitor of -tocopherol individually and also in roots at all stages of growth. Ascorbate is one of the under drought stress. most extensively studied anti-oxidant and has been 52

5 detected in the majority of plant cell types, organelles 2. Sankar, B., C. Abdul Jaleel, P. Manivannan, and apoplast [1,22]. Water stress resulted in significant increases in antioxidant AA concentration in Catharanthus roseus [23]. Triazole increased the level of antioxidants like ascorbic acid and -tocopherol like in Withania somnifera Dunal. seedlings and protected membrane by preventing or reducing oxidative damage [24]. Increase in ascorbic acid content was reported in the triadimefon treated Catharanthus roseus [25] and Withania somnifera [26]. A decrease in ascorbic acid was reported in ABA treatment in Ocimum sanctum plants [27]. - Tocopherol: -tocopherol of the drought stressed plants significantly increased when compared to control plants. Paclobutrazol caused an enhancement in -tocopherol content of roots under drought as well as well-watered conditions. ABA was an inhibitor of -tocopherol individually and also under drought stress. The active oxygen species formed at the membrane of leaves under drought stress was efficiently removed upon rehydration with increase in the -tocopherol and -carotene [28-3]. Soil applied propiconazole alleviated the impact of salinity on Catharanthus roseus by improving antioxidant status especially -tocopherol [31]. Alterations in biochemicals including non-enzymatic antioxidants in white yam (Dioscorea rotundata Poir.) under triazole fungicides application was previously reported [32]. CONCLUSION The non-enzymatic antioxidant molecules like ascorbate and -tocopherol showed significant increase under drought condition in Sesamum indicum. PBZ caused significant enhancement in these antioxidant enzymes under drought stress and also in well-watered conditions. It is not so with ABA treatment. ABA slightly reduced the non-enzymatic antioxidant contents. From the results, it can be concluded that, these growth regulators are better suited as stress ameliorating agents in gingelly. REFERENCES 1. Abdul Jaleel, C., R. Gopi, P. Manivannan, M. Gomathinayagam, Ksouri Riadh, Jallali Inès, Zhao Chang-Xing, Shao Hong-Bo and R. Panneerselvam, 29. Antioxidant defense responses: Physiological plasticity in higher plants under abiotic constraints. Acta Physiologiae Plantarum, 31(3): A. Kishorekumar, R. Somasundaram and R. Panneerselvam, 27. Effect of paclobutrazol on water stress amelioration through antioxidants and free radical scavenging enzymes in Arachis hypogaea L. Colloids and Surfaces B: Biointerfaces, 6: Abdul Jaleel, C., R. Gopi, P. Manivannan, M. Gomathinayagam, Shao Hong-Bo, Chang-Xing Zhao and R. Panneerselvam, 28. Endogenous hormonal and enzymatic responses of Catharanthus roseus with triadimefon application under water deficits. Comptes Rendus Biologies, 331: Abdul Jaleel, C., P. Manivannan, B. Sankar, A. Kishorekumar, R. Gopi, R. Somasundaram and R. Panneerselvam, 27. Pseudomonas fluorescens enhances biomass yield and ajmalicine production in Catharanthus roseus under water deficit stress. Colloids and Surfaces B: Biointerfaces, 6: Abdul Jaleel, C., P. Manivannan, G.M.A. Lakshmanan, M. Gomathinayagam and R. Panneerselvam, 28. Alterations in morphological parameters and photosynthetic pigment responses of Catharanthus roseus under soil water deficits. Colloids and Surfaces B: Biointerfaces, 61(2): Abdul Jaleel, C., B. Sankar, P.V. Murali, M. Gomathinayagam, G.M.A. Lakshmanan and R. Panneerselvam, 28. Water deficit stress effects on reactive oxygen metabolism in Catharanthus roseus; impacts on ajmalicine accumulation, Colloids and Surfaces B: Biointerfaces, 62(1): Abdul Jaleel, C., P. Manivannan, P.V. Murali, M. Gomathinayagam and R. Panneerselvam, 28. Antioxidant potential and indole alkaloid profile variations with water deficits along different parts of two varieties of Catharanthus roseus. Colloids and Surfaces B: Biointerfaces, 62: Abdul Jaleel, C., R. Gopi, B. Sankar, M. Gomathinayagam and R. Panneerselvam, 28. Differential responses in water use efficiency in two varieties of Catharanthus roseus under drought stress. Comptes Rendus Biologies, 331(1): Sankar, B., C. Abdul Jaleel, P. Manivannan, A. Kishorekumar, R. Somasundaram and R. Panneerselvam, 28. Relative efficacy of water use in five varieties of Abelmoschus esculentus (L.) Moench. under water-limited conditions. Colloids and Surfaces B: Biointerfaces, 62(1):

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