TRACE ELEMENTS IN URINE. Event #1, 2010
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1 TRACE ELEMENTS IN URINE Event #, 00 March 6, 00
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3 Event #, 00 Urine Arsenic The source of the test materials is human urine obtained from donor volunteers with informed consent. Urine was collected into polyethylene containers and then stored at 4 C. Following collection, urine from each donor was mixed and acidified to % v/v with nitric acid, and % (v/v) sulfamic acid was added to stabilize Hg. The urine was stored frozen at -80 ; after thawing at room temperature, precipitated salts were removed by centrifugation. The urine was separated into five pools and each was supplemented with different amounts of inorganic As 3+. Each pool was stirred for 4 hours to ensure thorough mixing prior to aliquoting 0-mL samples into acid-leached polypropylene vials. Samples were stored at -80 C prior to circulating for proficiency testing. The Target Value assigned for each PT material is the robust mean of the results reported by all participants in this event. The robust statistics were obtained utilizing algorithms based on those presented in ISO 358:005E Statistical methods for use in proficiency testing by interlaboratory comparisons. Values for urine arsenic range from 50.7 µg/l (0.68 µmol/l) to 78.8 µg/l (3.7 µmol/l). Acceptable ranges. The acceptable range is fixed at ±0% or ±6 µg/l for target values 30 µg/l. This provides a more realistic acceptability range at low concentrations of urine As, and the criteria are consistent with those in place for blood As. Discussion. Based upon the above criteria, 95.% of test results reported were judged as satisfactory, with one out of 5 participant laboratories (4.0%) reporting or more of the 5 results outside the acceptable ranges. Trace Elements PT Program
4 Urine Arsenic Test Results, 00 Event # ROBUST STATISTICAL SUMMARY TARGET VALUE ASSIGNMENT AND STATISTICS Results (µg/l urine) UE0-0 UE0-0 UE0-03 UE0-04 UE0-05 Robust Mean Robust Standard Deviation Standard Uncertainty RSD (%) Acceptable Range: Upper Limit Lower Limit notes: Results reported as less than the method detection limit are excluded from statistical calculations.
5 Urine Arsenic Test Results, 00 Event # PERFORMANCE OF PARTICIPATING LABORATORIES Lab Code Method UE0-0 Results (µg/l urine) UE0-0 UE0-03 UE0-04 UE0-05 Target Values: Info Only 07 DRC/CC-ICP-MS Info 09 ETAAS-Z Info 0 DRC/CC-ICP-MS ICP-MS DRC/CC-ICP-MS Info 47 ICP-MS Info 56 ICP-MS ICP-MS ICP-MS ICP-MS DRC/CC-ICP-MS ICP-MS Info 06 ICP-MS ICP-MS DRC/CC-ICP-MS Info 305 DRC/CC-ICP-MS ICP-MS DRC/CC-ICP-MS Info 359 ICP-MS ICP-MS Info 367 ICP-MS Info 385 DRC/CC-ICP-MS Info 39 DRC/CC-ICP-MS Info 395 DRC/CC-ICP-MS ETAAS other Info Percent satisfactory results for all participants: 95. % notes: reported outside upper limit reported outside lower limit Info : Unacceptable result notes: Results reported as less than the method detection limit are excluded from statistical calculations. only: results included for informational purposes only.
6 DRC/CC-ICP-MS Urine Arsenic Test Results, 00 Event # STATISTICAL SUMMARY BY METHOD ETAAS other ETAAS-Z ICP-MS All Laboratories Results (µg/l urine) UE0-0 UE0-0 UE0-03 UE0-04 UE notes: Insufficient data for calculation.
7 Event #, 00 Urine Cadmium The source of the test materials is human urine obtained from donor volunteers with informed consent. Urine was collected into polyethylene containers and then stored at 4 C. Following collection, urine from each donor was mixed and acidified to % v/v with nitric acid, and % (v/v) sulfamic acid was added to stabilize Hg. The urine was stored frozen at -80 ; after thawing at room temperature, precipitated salts were removed by centrifugation. The urine was separated into five pools and each was supplemented with different amounts of inorganic Cd +. Each pool was stirred for 4 hours to ensure thorough mixing prior to aliquoting 0-mL samples into acid-leached polypropylene vials. Samples were stored at -80 C prior to circulating for proficiency testing. The Target Value assigned for each PT material is the robust mean of the results reported by all participants in this event. The robust statistics were obtained utilizing algorithms based on those presented in ISO 358:005E Statistical methods for use in proficiency testing by interlaboratory comparisons. Values for urine cadmium range from.5 µg/l ( nmol/l) to 0. µg/l (79 nmol/l). Acceptable ranges. The acceptable range is fixed at ±5% or ± µg/l (9 nmol/l) around the target value whichever is greater. These criteria are used by the U.S. Occupational Safety and Health Administration (OSHA) to assess performance for occupational medicine. Discussion. Based upon the above criteria, 97.7% of test results reported were judged as satisfactory, with one of the 6 participant laboratories (3.8%) reporting or more of the 5 results outside the acceptable ranges. Trace Elements PT Program
8 Urine Cadmium Test Results, 00 Event # ROBUST STATISTICAL SUMMARY TARGET VALUE ASSIGNMENT AND STATISTICS Results (µg/l urine) UE0-0 UE0-0 UE0-03 UE0-04 UE0-05 Robust Mean Robust Standard Deviation Standard Uncertainty RSD (%) Acceptable Range: Upper Limit Lower Limit notes: Results reported as less than the method detection limit are excluded from statistical calculations.
9 Urine Cadmium Test Results, 00 Event # PERFORMANCE OF PARTICIPATING LABORATORIES Lab Code Method UE0-0 Results (µg/l urine) UE0-0 UE0-03 UE0-04 UE0-05 Target Values: Info Only 03 ICP-MS Info 07 DRC/CC-ICP-MS Info 0 ICP-MS ICP-MS ICP-MS Info 47 ICP-MS Info 56 ICP-MS ICP-MS ICP-MS ICP-MS DRC/CC-ICP-MS ICP-MS Info 06 ICP-MS ICP-MS ICP-MS Info 305 ICP-MS ICP-MS ICP-MS Info 339 HR-ICP-MS Info 359 ICP-MS ICP-MS Info 367 ETAAS-Z Info 385 ICP-MS Info 39 DRC/CC-ICP-MS Info 395 ICP-MS ETAAS other Info Percent satisfactory results for all participants: 97.7 % notes: reported outside upper limit reported outside lower limit Info : Unacceptable result notes: Results reported as less than the method detection limit are excluded from statistical calculations. only: results included for informational purposes only.
10 DRC/CC-ICP-MS Urine Cadmium Test Results, 00 Event # STATISTICAL SUMMARY BY METHOD ETAAS other ETAAS-Z HR-ICP-MS ICP-MS All Laboratories Results (µg/l urine) UE0-0 UE0-0 UE0-03 UE0-04 UE notes: Insufficient data for calculation.
11 Event #, 00 Urine Mercury The source of the test materials is human urine obtained from donor volunteers with informed consent. Urine was collected into polyethylene containers and then stored at 4 C. Following collection, urine from each donor was mixed and acidified to % v/v with nitric acid, and % (v/v) sulfamic acid was added to stabilize Hg. The urine was stored frozen at -80 ; after thawing at room temperature, precipitated salts were removed by centrifugation. The urine was separated into five pools and each was supplemented with different amounts of inorganic Hg. Each pool was stirred for 4 hours to ensure thorough mixing prior to aliquoting 0-mL samples into acid-leached polypropylene vials. Samples were stored at -80 C prior to circulating for proficiency testing. The Target Value assigned for each PT material is the robust mean of the results reported by all participants in this event. The robust statistics were obtained utilizing algorithms based on those presented in ISO 358:005E Statistical methods for use in proficiency testing by interlaboratory comparisons. Values for urine mercury range from 33.9 µg/l (69 nmol/l) to 4. µg/l (69 nmol/l). Acceptable ranges. The acceptable range is fixed at ±30% or ±3 µg/l (5 nmol/l) for target values 0 µg/l. The criteria are consistent with those in place for blood Hg. Discussion. Based upon the above criteria, 93.8% of test results reported were judged as satisfactory, with two of the 6 participant laboratories (7.7%) reporting or more of the 5 results outside the acceptable ranges. Trace Elements PT Program
12 Urine Mercury Test Results, 00 Event # ROBUST STATISTICAL SUMMARY TARGET VALUE ASSIGNMENT AND STATISTICS Results (µg/l urine) UE0-0 UE0-0 UE0-03 UE0-04 UE0-05 Robust Mean Robust Standard Deviation Standard Uncertainty RSD (%) Acceptable Range: Upper Limit Lower Limit notes: Results reported as less than the method detection limit are excluded from statistical calculations.
13 Urine Mercury Test Results, 00 Event # PERFORMANCE OF PARTICIPATING LABORATORIES Lab Code Method UE0-0 Results (µg/l urine) UE0-0 UE0-03 UE0-04 UE0-05 Target Values: Info Only 03 ICP-MS Info 07 DRC/CC-ICP-MS Info 09 AFS Info 0 ICP-MS ICP-MS CV-AAS Info 56 ICP-MS ICP-MS ICP-MS ICP-MS DRC/CC-ICP-MS ICP-MS Info 06 ICP-MS CV-AAS ICP-MS Info 305 ICP-MS ICP-MS CV-AAS Info 339 HR-ICP-MS Info 359 ICP-MS ICP-MS Info 367 CV-AAS Info 39 CV-AAS Info 395 ICP-MS CV-AAS Info 448 CV-AAS Info Percent satisfactory results for all participants: 93.8 % notes: reported outside upper limit reported outside lower limit Info : Unacceptable result notes: Results reported as less than the method detection limit are excluded from statistical calculations. only: results included for informational purposes only.
14 AFS Urine Mercury Test Results, 00 Event # STATISTICAL SUMMARY BY METHOD CV-AAS DRC/CC-ICP-MS HR-ICP-MS ICP-MS All Laboratories Results (µg/l urine) UE0-0 UE0-0 UE0-03 UE0-04 UE notes: Insufficient data for calculation.
15 Event #, 00 Urine Lead The source of the test materials is human urine obtained from donor volunteers with informed consent. Urine was collected into polyethylene containers and then stored at 4 C. Following collection, urine from each donor was mixed and acidified to % v/v with nitric acid, and % (v/v) sulfamic acid was added to stabilize Hg. The urine was stored frozen at -80 ; after thawing at room temperature, precipitated salts were removed by centrifugation. The urine was separated into five pools and each was supplemented with different amounts of inorganic Pb +. Each pool was stirred for 4 hours to ensure thorough mixing prior to aliquoting 0-mL samples into acid-leached polypropylene vials. Samples were stored at -80 C prior to circulating for proficiency testing. The Target Value assigned for each PT material is the robust mean of the results reported by all participants in this event. The robust statistics were obtained utilizing algorithms based on those presented in ISO 358:005E Statistical methods for use in proficiency testing by interlaboratory comparisons. Values for urine lead range from 35.0 µg/l (0.7 µmol/l) to 93. µg/l (.4 µmol/l). Acceptable ranges. The acceptable range is fixed at ±0% or ±40 µg/l (0.9 µmol/l) around the target value, whichever is greater. These criteria are consistent with those established under CLIA 88 (Federal Register Volume 57, Number 40, 493. and , February 8, 99) for blood lead. Discussion. Based upon the above criteria, 95.4% of test results were judged as satisfactory, with one of the 6 participant laboratories (3.8%) reporting or more of the 5 results outside the acceptable ranges. Trace Elements PT Program
16 Urine Lead Test Results, 00 Event # ROBUST STATISTICAL SUMMARY TARGET VALUE ASSIGNMENT AND STATISTICS Results (µg/l urine) UE0-0 UE0-0 UE0-03 UE0-04 UE0-05 Robust Mean Robust Standard Deviation Standard Uncertainty RSD (%) Acceptable Range: Upper Limit Lower Limit notes: Results reported as less than the method detection limit are excluded from statistical calculations.
17 Urine Lead Test Results, 00 Event # PERFORMANCE OF PARTICIPATING LABORATORIES Lab Code Method UE0-0 Results (µg/l urine) UE0-0 UE0-03 UE0-04 UE0-05 Target Values: Info Only 03 ICP-MS Info 07 DRC/CC-ICP-MS Info 0 ICP-MS ETAAS-Z Info 4 ICP-MS ICP-MS Info 47 ICP-MS Info 56 ICP-MS ICP-MS ICP-MS ICP-MS DRC/CC-ICP-MS ICP-MS Info 06 ICP-MS ICP-MS ICP-MS Info 305 ICP-MS ICP-MS ICP-MS Info 339 HR-ICP-MS Info 359 ICP-MS ICP-MS Info 383 ETAAS-Z ICP-MS Info 39 ETAAS other Info 395 ICP-MS Percent satisfactory results for all participants: 95.4 % notes: reported outside upper limit reported outside lower limit Info : Unacceptable result notes: Results reported as less than the method detection limit are excluded from statistical calculations. only: results included for informational purposes only.
18 DRC/CC-ICP-MS ETAAS other ETAAS-Z HR-ICP-MS ICP-MS All Laboratories Urine Lead Test Results, 00 Event # STATISTICAL SUMMARY BY METHOD Results (µg/l urine) UE0-0 UE0-0 UE0-03 UE0-04 UE notes: Insufficient data for calculation.
19 Event #, 00 Additional Trace Elements Reported in Urine Participating laboratories reported analytical results for any other elements that are routinely reported in order to characterize these materials more completely. Results and descriptive statistics are provided for reference purposes. No target value or acceptable range is implied. As, Cd, and Pb were spiked using a stock standard containing all elements in the National Health and Nutritional Examination Survey (NHANES) conducted by the Centers for Disease Control and Prevention. Refer to for more information on recent NHANES data for these elements in urine. In addition, these samples were spiked with leading elements present in other proficiency testing programs. The following table shows the additional elements spiked in the samples. NHANES Elements Ba Be Co Cs Mo Pt Sb Tl U W Additional Elements Al Cr Cu Mn Ni Se Sn Te V Zn Trace Elements PT Program
20 Urine Additional Elements, 00 Event # Page Urine Aluminum Results (µg/l) Lab Code Method UE0-0 UE0-0 UE0-03 UE0-04 UE ICP-MS DRC/CC-ICP-MS ICP-MS < ICP-MS ICP-MS ICP-MS Arithmetic Mean, n= SD Urine Barium Results (µg/l) Lab Code Method UE0-0 UE0-0 UE0-03 UE0-04 UE ICP-MS ICP-MS ICP-MS ICP-MS ICP-MS ICP-MS ICP-MS Arithmetic Mean, n= SD Urine Beryllium Results (µg/l) Lab Code Method UE0-0 UE0-0 UE0-03 UE0-04 UE ICP-MS ICP-MS ICP-MS ICP-MS ICP-MS ICP-MS Arithmetic Mean, n= SD
21 Urine Additional Elements, 00 Event # Page Urine Cobalt Results (µg/l) Lab Code Method UE0-0 UE0-0 UE0-03 UE0-04 UE ICP-MS ICP-MS ICP-MS ICP-MS ICP-MS ICP-MS ICP-MS ICP-MS DRC/CC-ICP-MS Arithmetic Mean, n= SD Urine Chromium Results (µg/l) Lab Code Method UE0-0 UE0-0 UE0-03 UE0-04 UE DRC/CC-ICP-MS DRC/CC-ICP-MS ETAAS-Z DRC/CC-ICP-MS DRC/CC-ICP-MS DRC/CC-ICP-MS ICP-MS DRC/CC-ICP-MS DRC/CC-ICP-MS Arithmetic Mean, n= SD
22 Urine Additional Elements, 00 Event # Page 3 Urine Cesium Results (µg/l) Lab Code Method UE0-0 UE0-0 UE0-03 UE0-04 UE ICP-MS ICP-MS ICP-MS ICP-MS ICP-MS ICP-MS Arithmetic Mean, n= SD Urine Copper Results (µg/l) Lab Code Method UE0-0 UE0-0 UE0-03 UE0-04 UE ICP-MS ICP-MS ICP-MS DRC/CC-ICP-MS ICP-MS ICP-MS ICP-MS ICP-MS > Arithmetic Mean, n= SD Urine Manganese Results (µg/l) Lab Code Method UE0-0 UE0-0 UE0-03 UE0-04 UE ICP-MS ICP-MS DRC/CC-ICP-MS ICP-MS ICP-MS ICP-MS ICP-MS DRC/CC-ICP-MS Arithmetic Mean, n= SD
23 Urine Additional Elements, 00 Event # Page 4 Urine Molybdenum Results (µg/l) Lab Code Method UE0-0 UE0-0 UE0-03 UE0-04 UE ICP-MS ICP-MS ICP-MS ICP-MS ICP-MS ICP-MS ICP-MS ICP-MS ICP-MS (39) (DRC/CC-ICP-MS) (85.4) (43.5) (35.6) (35.9) (6.) Arithmetic Mean, n=9 (outliers deleted) SD Urine Nickel Results (µg/l) Lab Code Method UE0-0 UE0-0 UE0-03 UE0-04 UE ICP-MS ICP-MS ICP-MS ICP-MS ICP-MS ICP-MS ICP-MS ICP-MS (39) (DRC/CC-ICP-MS) (9.6) (59.9) (6.) (5.7) (3.0) Arithmetic Mean, n=8 (outliers deleted) SD
24 Urine Additional Elements, 00 Event # Page 5 Urine Platinum Results (µg/l) Lab Code Method UE0-0 UE0-0 UE0-03 UE0-04 UE ICP-MS ICP-MS ICP-MS ICP-MS ICP-MS ICP-MS Arithmetic Mean, n= SD Urine Antimony Results (µg/l) Lab Code Method UE0-0 UE0-0 UE0-03 UE0-04 UE ICP-MS ICP-MS ICP-MS ICP-MS ICP-MS ICP-MS ICP-MS ICP-MS (39) (DRC/CC-ICP-MS) (6.3) (6.6) (8.6) (44.) (3.) Arithmetic Mean, n=8 (outliers deleted) SD
25 Urine Additional Elements, 00 Event # Page 6 Urine Selenium Results (µg/l) Lab Code Method UE0-0 UE0-0 UE0-03 UE0-04 UE DRC/CC-ICP-MS DRC/CC-ICP-MS ICP-MS DRC/CC-ICP-MS ICP-MS < ICP-MS ICP-MS ICP-MS DRC/CC-ICP-MS (39) (DRC/CC-ICP-MS) (05.9) (59.7) (35.4) (5.9) (63.6) Arithmetic Mean, n=9 (outliers deleted) SD Urine Tin Results (µg/l) Lab Code Method UE0-0 UE0-0 UE0-03 UE0-04 UE ICP-MS ICP-MS ICP-MS ICP-MS ICP-MS Arithmetic Mean, n= SD Urine Tellurium Results (µg/l) Lab Code Method UE0-0 UE0-0 UE0-03 UE0-04 UE ICP-MS ICP-MS ICP-MS ICP-MS Arithmetic Mean, n= SD
26 Urine Additional Elements, 00 Event # Page 7 Urine Thallium Results (µg/l) Lab Code Method UE0-0 UE0-0 UE0-03 UE0-04 UE ICP-MS ICP-MS ICP-MS ICP-MS ICP-MS < ICP-MS ICP-MS ICP-MS > ICP-MS DRC/CC-ICP-MS Arithmetic Mean, n= SD Urine Uranium Results (µg/l) Lab Code Method UE0-0 UE0-0 UE0-03 UE0-04 UE ICP-MS ICP-MS ICP-MS ICP-MS <.0 <.0.7 <.0 3 ICP-MS ICP-MS ICP-MS ICP-MS (39) (DRC/CC-ICP-MS) (0) (0.3) (0) (.6) (0.0) Arithmetic Mean, n=8 (outliers deleted) SD
27 Urine Additional Elements, 00 Event # Page 8 Urine Vanadium Results (µg/l) Lab Code Method UE0-0 UE0-0 UE0-03 UE0-04 UE DRC/CC-ICP-MS DRC/CC-ICP-MS ICP-MS DRC/CC-ICP-MS Arithmetic Mean, n= SD Urine Tungsten Results (µg/l) Lab Code Method UE0-0 UE0-0 UE0-03 UE0-04 UE ICP-MS ICP-MS ICP-MS ICP-MS ICP-MS ICP-MS ICP-MS Arithmetic Mean, n= SD Urine Zinc Results (µg/l) Lab Code Method UE0-0 UE0-0 UE0-03 UE0-04 UE ICP-MS ICP-MS ICP-MS DRC/CC-ICP-MS ICP-MS ICP-MS ICP-MS ICP-MS Arithmetic Mean, n= SD
28 ATOMIC SPECTROMETRY METHODS Trace Elements in Urine METHOD NOTES A- ETAAS-Z (Electrothermal atomic absorption spectrometry with Zeeman background correction) A- ETAAS other (i.e., D, S-H background correction) A-3 FAAS (Flame atomic absorption spectrometry) A-4 CV-AAS (Cold vapor atomic absorption spectrometry) A-5 HG-AAS (Hydride generation atomic absorption spectrometry) A-6 AFS (Atomic fluorescence spectrometry) A-7 Other INDUCTIVELY COUPLED PLASMA P- ICP-MS (Inductively coupled plasma - mass spectrometry) P- DRC/CC-ICP-MS (ICP-MS used in the Dynamic Reaction Cell or Collision Cell mode) P-3 ICP-AES/OES (ICP atomic/optical emission spectrometry) P-4 HR-ICP-MS (High resolution ICP-MS) P-5 ETV-ICP-MS (Electrothermal vaporization ICP-MS) P-6 ID-ICP-MS (Isotope dilution ICP-MS) P-7 Other ELECTROCHEMICAL METHODS E- ASV (Anodic stripping voltammetry without digestion) E- ASV-LeadCare (Anodic stripping voltammetry using the ESA LeadCare system) E-3 Fluoride specific electrode E-4 Other MOLECULAR FLUORIMETRY F- EtOAc (Ethyl acetate-acetic acid extraction method for determination of erythrocyte protoporphyrin) F- Aviv hematofluorometry (for determination of EP at hematocrit 35) F-3 Helena ZPP (for determination of zinc protoporphyrin in µmol ZPP/mol heme) F-4 Other OTHER METHODS If your method is not listed in the above list, please describe it briefly.
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