Non-protein nitrogen. balance, was dissolved in approximately 50 cc. of distilled water. By the method of Folin and Wu. 251
|
|
- Ellen Arnold
- 6 years ago
- Views:
Transcription
1 SOME CHEMICAL STUDIES OF COMMERCIAL BACTERIOLOGICAL PEPTONES JAMES G. McALPINE AND GEORGE D. BRIGHAM From the Department of Animal Diseases, Storrs Agricultural Experiment Station Storrs, Connecticut Received for publication May 29, 1928 I. INTRODUCTION In the course of a study of the abortus-melitensis group it was found that certain commercial peptones were better suited to initiate growth and multiplication than others. It was decided, therefore, to make as accurate chemical analyses as present methods will permit of certain well-known commercial brands of peptones in order to determine in what form nitrogen may be best utilized by the members of this bacterial group. II. METHODS Four commercial brands of peptone were employed in this study: Fairchild's, Difco-Bacto, Difco-Proteose and Witte's. One gram of the peptone, accurately weighed on a delicate balance, was dissolved in approximately 50 cc. of distilled water and sterilized in the autoclave for fifteen minutes at 15 pounds extra pressure. The peptone solution was carefully transferred to a 100 cc. volumetric flask and the whole made up to the mark. The following nitrogen determinations were made on aliquot portions of this solution. Five-cubic-centimeter amounts were used in all of the determinations except those for non-protein nitrogen and amino nitrogen by the Sorensen method where 20 cc. and 1 cc. quantities were used respectively. Total nitrogen. method. Non-protein nitrogen. By the Gunning modification of the Kjeldhal By the method of Folin and Wu. 251
2 252 JAMES G. McALPINE AND GEORGE D. BRIGHAM Free-ammonia. By the Van Slyke and Cullen modification of the Folin air current process. Amino-nitrogen. By the Van Slyke and Sorensen methods. The S6rensen results were corrected for free ammonia. The Van Slyke determinations were made on the ammonia-free material. Protein-nitrogen. This was determined by subtracting the non-protein nitrogen from the total nitrogen figure. Polypeptid nitrogen. This nitrogen fraction is represented as the difference between the non-protein nitrogen figure and the sum of the amino and free ammonia nitrogen. The Van Slyke figure was used here for the amino nitrogen. Two different lots of each peptone were analyzed, and all determinations were made in duplicate. The figures obtained from the two different lots of the same brand of peptone were averaged. It was found that the different lots varied only slightly, the greatest variation being found in the two lots of Witte's peptone. III. EXPERIMENTAL The results are given in the accompanying chart in which the different fractions are drawn to scale, with the exception of the free ammonia figures. Owing to the small amount of free ammonia, it was necessary to multiply the scale by ten in this case in order to make the results legible. Total nitrogen. It will be seen that the amounts of total nitrogen do not vary very greatly in these four commercial peptones, Witte, with 152 mgm., is the highest, and Difco-Proteose, with 125 mgm., is the lowest. Non-protein nitrogen. In the next figure on the chart are given the results of the non-protein nitrogen determinations. Witte's peptone which shows the largest amount of total nitrogen has the smallest amount of the non-protein fraction (56.17 mgm.). In the Difco-Bacto peptone the total nitrogen and non-protein nitrogen are not very far from equal. Difco-Proteose peptone has distinctly more non-protein nitrogen (69.3 mgm.) than Witte's, and in the Fairchild peptone, almost three-fourths of the total nitrogen is non-protein (102.5 mgm.).
3 CHEMICAL STUDIES OF BACTERIOLOGICAL PEPTONES 253 Protein-nitrogen. These figures were not obtained by actual determination, but by the subtraction of the non-protein nitrov r T0t./ N Van S(yk 11 ANon-Proten Nl Pkiern N ad to ṾI?r.ee NH, ^P.rpo.tid /V CHART 1 gen figures from the total nitrogen results. The chart shows that the Witte peptone contains the largest amount of protein nitrogen (93.42 mgm.), and that the Difco-Proteose has next to the
4 254 JAMES G. McALPINE AND GEORGE D. BRIGHAM largest (54.6 mgm.), though its protein nitrogen content is less than two-thirds that of the foreign brand. The protein nitrogen in Fairchild peptone is comparatively small (24.2 mgm.) and that in Difco-Bacto is almnost negligible (8.4 mgm.). Polypeptid nitrogen. This fraction also was not obtained by actual determination, but by subtractmg the sum of the amino and free ammonia nitrogen figures from the amount of non-protein nitrogen. Difco-Bacto shows the largest amount of polypeptid nitrogen (82.17 mgm.) and Fairchild's next to the largest (79.57 mgm.). There was correspondingly much less polypeptid nitrogen in the Witte and Difco-Proteose peptones. Amino nitrogen. By the Van Slyke determination the Difco- Bacto, the Difco-Proteose and the Fairchild peptone were found to have about equal amounts of amino nitrogen (approximately 48.0 mgm.), while the Witte peptone contained only about twothirds as much (29.65 mgm.). The Sorensen results are more irregular, indicating perhaps that the di-amino acids were present in greater quantities in some of the brands than in others. Free ammonia. With the exception of the Fairchild peptone, the amount of free ammonia in any of these peptones was almost negligible. It should be remembered that in the chart these amounts are drawn to a scale ten times greater than that used for the other determinations. IV. DISCUSSION The results given in this paper must be regarded merely as preliminary in the main. It is realized that if more lots of each of the peptones had been analyzed the relative amounts of the various fractions might have been expressed in somewhat different figures. However, as previously stated, the only large variations that were found were in the two lots of the Witte peptone. Although the amounts of total nitrogen in all of these peptones appeared to be about equal, the different nitrogen fractions in the various brands differed greatly from each other. It is possible, in a measure at least, to estimate the length of the digestion period and the type of the enzyme employed in the production of peptone, from the above determinations. Relatively long diges-
5 CHEMICAL STUDIES OF BACTERIOLOGICAL PEPTONES 255 tion with trypsin, particularly after continued preliminary treatment with pepsin, will of course yield relatively large amounts of non-protein, particularly amino, nitrogen. On the other hand, digestion with pepsin alone, or followed by a very short tryptic process, will furnish a product high in "protein" (proteose) nitrogen. Practically all of the total nitrogen in the Difco-Bacto product was "non-protein" in character. There was a relatively large amount of polypeptids present, and according to the Van Slyke method the amino nitrogen fraction was high. There appeared to be very little free ammonia and "protein" nitrogen in this brand. The non-protein nitrogen content of Witte's peptone was comparatively small, and was divided about equally between the polypeptid and amino nitrogen fractions. The amount of free ammonia was very small. A large portion of the total nitrogen was of the proteose type. In the Difco-Proteose peptone the total nitrogen was about equally divided between the non-protein nitrogen and the protein nitrogen fractions. About one-third of the non-protein nitrogen was apparently polypeptid and two-thirds amino acid in character. The protein nitrogen was high and the free ammonia was practically negligible. The Fairchild peptone contained a relatively large amount of non-protein nitrogen which was about equally divided between the polypeptid and amino nitrogen (Van Slyke) fractions. The amount of protein nitrogen was small. Evidently digestion is carried on for a longer time in the preparation of this peptone than with Witte and Difco-Proteose peptones. The free ammonia determinations were high. If it may be assumed that the protein nitrogen is practically all proteose in character, the Witte product contains a very large quantity of this product. Difco-Proteose peptone has about half as much as Witte's, while the Fairchild and Difco-Bacto peptones contain relatively small amounts of this nitrogen partition. This is especially interesting in view of the more recent work showing that proteoses appear to be necessary for toxin production. Gibbs
6 256 JAMES G. McALPINE AND GEORGE D. BRIGHAM and Rettger (1927) obtained potent toxins with both the Difco- Proteose and Witte peptone, and they showed that one fraction, evidently proteose in character, was necessary to obtain this result. The results given above corroborate the statement made by Hucker and Carpenter (1927). "The original 'Witte peptone' contained a large amount of proteose and was only slightly digested in comparison with other brands. 'Fairchild's peptone,' on the other hand, is most completely digested. 'Bacto peptone' appears to be intermediate between Witte and Fairchild's." V. SUMMARY 1. Four different brands of commercial peptone were analyzed quantitatively for their various nitrogen fractions. Although the total nitrogen present in all of these different commercial brands was practically the same, there was a marked difference in the amounts of the various nitrogen fractions. 2. Witte and Difco-Proteose peptones contain the largest amount of so-called "protein" nitrogen. It is probable that most of this nitrogen is proteose in character. REFERENCES GIBBS, C. S., AND RETTGER, L. F Some factors governing the production of diphtheria toxin in artificial media. Jour. Immunol., 13, IluCKER, G. J., AND CARPENTER, D. C The relation of hydrolytic decomposition products of protein to bacterial growth. Jour. Inf. Dis., 40,
Wallace and Neave (1927), and Tittsler (1928) reported reduction by Sal. pullorum. Hadley, Elkins and Caldwell (1918) found no
THE REDUCTION OF NITRATES TO NITRITES BY SAL- MONELLA PULLORUM AND SALMONELLA GALLINARUMi Division of Bacteriology, Penn8ylvania State College, State College, Pa. Received for publication October 9, 1929
More informationBACTERIA. media for bacteria highly desirable. Douglas and Gordon in England, and more recently Meyer in this country, have proposed
YEAST AUTOLYSATE AS A CULTURE MEDIUM FOR BACTERIA I. J. KLIGLER From the Laboratories of the Rockefeller Institute for Medical Research Received for publication November 23, 1918 The necessity for conserving
More informationfor Medical Research. (Received May 10th, 1922.)
XLV. NOTE ON URINARY TIDES AND EXCRETORY RHYTHM. BY JAMES ARGYLL CAMPBELL AND THOMAS ARTHUR WEBSTER. From the Department of Applied Physiology, National Institute for Medical Research. (Received May 10th,
More informationby both esterification and acetylation of the liver concentrate inorganic salts and a source of energy such as glycerol or
BETA ALANINE AS A GROWTH ACCESSORY FOR THE DIPHTHERIA BACILLUS J. HOWARD MUELLER AND SIDNEY COHEN Department of Bacteriology and Immunology, Harvard University Medical School, Boston, Massachusetts Received
More informationTHE EFFECT OF VARIOUS ACIDS ON THE DIGESTION OF PROTEINS BY PEPSIN.
Published Online: 20 July, 1919 Supp Info: http://doi.org/10.1085/jgp.1.6.607 Downloaded from jgp.rupress.org on August 20, 2018 THE EFFECT OF VARIOUS ACIDS ON THE DIGESTION OF PROTEINS BY PEPSIN. BY J.
More informationprotein (Eaton 1936 a, 1937; Pappenheimer 1937). If other
COMPARATIVE STUDIES ON THE PURIFICATION OF TETANUS AND DIPHTHERIA TOXINS MONROE D. EATON AND AXEL GRONAU Department of Bacteriology, Washington University School of Medicine, St. Louis, Missouri Received
More informationCONTENT OF ICE CREAM MIX' A. C. FAY AND N. E. OLSON Kansas State Agricultural College. Received for publication, April 15, 1927 INTRODUCTION
THE EFFECT OF GELATIN ON THE BACTERIAL CONTENT OF ICE CREAM MIX' A. C. FAY AND N. E. OLSON Kansas State Agricultural College Received for publication, April 15, 197 INTRODUCTION During the past few years
More informationExperimental. Schmidt, in his experiments, boiled his solutions
PROTECTION OF TRYPSIN FROM DESTRUCTION BY HEAT. BY D. IL DE SOUZA. (From the Institute of Physiology, University College, London.) E. W. SCHMIDT' has recently claimed: that trypsin in the presence of peptone,
More informationConsequently, the authors decided to investigate the various A STUDY OF METHODS FOR THE DETERMINATION OF
A STUDY OF METHODS FOR THE DETERMINATION OF REDUCING SUGARS IN BACTERIAL CULTURES COLORIMETRIC METHODS DOROTHEA KLEMME AND CHARLES F. POE Division of Sanitary Chemistry, Department of Chemistry, University
More informationSTUDIES ON THE PROTEOLYTIC BACTERIA OF MILK
STUDIES ON THE PROTEOLYTIC BACTERIA OF MILK III. ACTION OF PROTEOLYTIC BACTERIA OF MILK ON CASEIN AND GELATIN WILLIAM C. FRAZIER AND PHILIP RUPP From the Research Laboratories, Bureau of Dairy Industry,
More informationwhether or not acid cleavage products were formed from the STUDIES ON CARBON METABOLISM OF ORGANISMS
STUDIES ON CARBON METABOLISM OF ORGANISMS OF THE GENUS MYCOBACTERIUM III. END PRODUCTS OF CARBOHYDRATE UTILIZATION AS DETERMINED IN SYNTHETIC MEDIA CULTURES MALCOLM H. MERRILL Department of Bacteriology
More information3, 4), although its concentration in mixed gastric
THE VALUE OF THE ACID TEST MEAL: A STUDY OF NORMAL PERSONS AND OF PERSONS WITH DUODENAL ULCER By C. STUART WELCH AND MANDRED W. COMFORT (From The Mayo Foundation and the Division of Medicine, The Mayo
More informationTHE NORMAL LIMITS OF VARIATION OF THE METHY- LENE-BLUE REDUCTION TEST '
THE NORMAL LIMITS OF VARIATION OF THE METHY- LENE-BLUE REDUCTION TEST ' By A. C. FAY Dairy Bacteriologist^ Kansas Agricultural Experiment Station INTRODUCTION The methylene-blue reduction or reducíase
More informationTHE CREATININE LEVEL OF BLOOD SERUM AS AN INDICATOR OF CARCASS COMPOSITION 1 PAUL R. WUTHIER AND P. O. STRATTON
THE CREATININE LEVEL OF BLOOD SERUM AS AN INDICATOR OF CARCASS COMPOSITION 1 PAUL R. WUTHIER AND P. O. STRATTON Wyoming Agricultural Experiment Station URRENT trends in consumer demand emphasize the importance
More informationSTUDIES ON HEMOGLOBIN. III An Ultra-Micro-method for the Determination of Hemoglobin as a Peroxidase.
BY STUDIES ON HEMOGLOBIN. III An Ultra-Micro-method for the Determination of Hemoglobin as a Peroxidase. HSIEN WU (From the L' b)oratory of Physiological CCemistry. Peking Union Medical College, Peking)
More informationThe slime or gum produced by Azotobacter chroococcum has. (1926). Buchanan (1909) in a discussion of gum production
GUM PRODUCTION BY AZOTOBACTER CHROOCOC- CUM OF BEIJERINCK AND ITS COMPOSITION' W. BROOKS HAMILTON Department of Bacteriology, MacDonald College, Quebec, Canada Received for publication, March 5, 1931 INTRODUCTION
More informationthe following objectives: 1. Describe the environment of the stomach and its relation to protein digestion.
Unit B: Understanding Animal Body Systems Lesson 5: Protein Digestion by Enzyme Action Student Learning Objectives: Instruction in this lesson should result in students achieving the following objectives:
More informationvolume and surface area. Walker and Winslow (1932) reported metabolic rates per cell being observed towards the end of the
A COMPARISON OF THE METABOLIC ACTIVITIES OF AEROBACTER AEROGENES, EBERTHELLA TYPHI AND ESCHERICHIA COLI C. E. CLIFTON Department of Bacteriology and Experimental Pathology, Stanford University, California
More informationRICINOLEATE UPON BACTERIA
A COMPARATIVE STUDY OF THE ACTION OF SODIUM RICINOLEATE UPON BACTERIA From the Division of Laboratories and Research, New York State Department of Health, Albany Received for publication, May 14, 1928
More informationTHE ASSIMILATION OF AMMONIA NITROGEN BY THE TOBACCO PLANT: A PRELIMINARY STUDY WITH ISOTOPIC NITROGEN. (Received for publication, July 3, 1940)
THE ASSIMILATION OF AMMONIA NITROGEN BY THE TOBACCO PLANT: A PRELIMINARY STUDY WITH ISOTOPIC NITROGEN BY HUBERT BRADFORD VICKERY AND GEORGE W. PUCHER (Prom the Biochemical Laboratory of the Connecticut
More informationANTI-TRYPTIC ACTIVITY OF SYNOVIAL FLUID IN PATIENTS
ANTI-TRYPTIC ACTIVITY OF SYNOVIAL FLUID IN PATIENTS WITH VARIOUS TYPES OF ARTHRITIS 1 BY WILLIAM F. HOLMES, JR., CHESTER S. KEEFER AND WALTER K. MYERS (From the Thorndike Memorial Laboratory, Second and
More informationTHE EFFECTS OF ACIDITY UPON THE GROWTH OF PNEUMOCOCCUS IN CULTURE MEDIA CONTAINING PROTEINS
THE EFFECTS OF ACIDITY UPON THE GROWTH OF PNEUMOCOCCUS IN CULTURE MEDIA CONTAINING PROTEINS BY WILLIAM H. KELLEY, M.D. (From the Department of Medicine of the Duke University School of Medicine, Durham,
More informationFERTILIZER CONTROL IN 1920
FERTILIZER CONTROL IN 1920 C. O. SWANSON AND W. L. LATSHAW EXPLANATORY STATEMENTS In compliance with the Kansas fertilizer law, samples of the different brands of fertilizer sold the state are each year
More informationTHE ESTIMATION OF PEPSIN, TRYPSIN, PAPAIN, AND CATHEPSIN WITH HEMOGLOBIN
Published Online: 20 September, 1938 Supp Info: http://doi.org/10.1085/jgp.22.1.79 Downloaded from jgp.rupress.org on July 1, 2018 THE ESTIMATION OF PEPSIN, TRYPSIN, PAPAIN, AND CATHEPSIN WITH HEMOGLOBIN
More informationTHE ESTIMATION OF TRYPSIN WITH HEMOGLOBIN
THE ESTIMATION OF TRYPSIN WITH HEMOGLOBIN BY M. L. ANSON Am) A. E. MIRSKY (From the Laboratories of The Rockefeller Institute for Medical Research, Princeton, N. J., and the Hospital of The Rockefeller
More informationlactose-fermenting variants (reds). Appreciable lactose utilization variants. Hershey and Bronfenbrenner (1936) found the non-lactosefermenting
THE LACTASE ACTIVITY OF ESCHERICHIA COLI- MUTABILE' CHARLES J. DEERE, ANNA DEAN DULANEY AND I. D. MICHELSON Department of Chemistry and Department of Bacteriology, University of Tennessee School of Biological
More informationpossibilities occurs. It has been found that the organism acquires addition of vitamin B1 to cells of P. pentosaceum which had
ADAPTATION OF THE PROPIONIC-ACID BACTERIA TO VITAMIN B1 SYNTHESIS INCLUDING A METHOD OF ASSAY M. SILVERMAN AND C. H. WERKMAN Bacteriology Section, Industrial Science Research Institute, Iowa State College,
More informationTHE EFFECT OF COOKING ON THE DIGESTIBILITY OF MEAT.
CXCII. THE EFFECT OF COOKING ON THE DIGESTIBILITY OF MEAT. By WINIFRED MARY CLIFFORD. From the Physiological Laboratory, King's College of Household and Social Science, Kensington, London, W. 8. (Received
More informationPROTEOSE INTOXICATIONS AND INJURY OF BODY PROTEIN. (Received for publication, March 15, 1918.)
Published Online: 1 August, 1918 Supp Info: http://doi.org/10.1084/jem.28.2.213 Downloaded from jem.rupress.org on January 13, 2019 PROTEOSE INTOXICATIONS AND INJURY OF BODY PROTEIN. III. ToxIc PROTEIN
More informationEFFECTS ON THE THERMAL RESISTANCE OF BACTERIA. (1934). This author found that sucrose and glucose in concentrations
HEAT STERILISED REDUCING SUGARS AND THEIR EFFECTS ON THE THERMAL RESISTANCE OF BACTERIA J. G. BAUMGARTNER Research Laboratory, 36, Crimscott Street, London, S.E.1., England Received for publication March
More informationA MICRO TIME METHOD FOR DETERMINATION OF REDUCING SUGARS, AND ITS APPLICATION TO ANALYSIS OF BLOOD AND URINE.
A MICRO TIME METHOD FOR DETERMINATION OF REDUCING SUGARS, AND ITS APPLICATION TO ANALYSIS OF BLOOD AND URINE. BY JAMES A. HAWKINS. (From Ike Hospital of The Rockefeller Institute for Medical Research,
More informationA MODIFIED FOLIN AND WU BLOOD SUGAR METHOD.
A MODIFIED FOLIN AND WU BLOOD SUGAR METHOD. BY VERA E. ROTHBERG AND FRANK A. EVANS. (From the William H. Singer Memorial Research Laboratory, Pittsburgh.) (Received for publication, August 7, 1923.) In
More informationTHE COLORIMETRIC DETERMINATION OF TOTAL PHOSPHOROUS IN PLANT SOLUTIONS.*
THE COLORIMETRIC DETERMINATION OF TOTAL PHOSPHOROUS IN PLANT SOLUTIONS.* R. W. GERDEL.f INTRODUCTION. A review of the literature reveals a number of methods for colorimetric determination of the phosphorous
More informationTHE DETERMINATION OF THE AMOUNT OF CON- NECTIVE TISSUE IN MEAT.
THE DETERMINATION OF THE AMOUNT OF CON- NECTIVE TISSUE IN MEAT. BY H. H. MITCHELL, R. L. ZIMMERMAN, AND T. S. HAMILTON. (From the Division of Animal Nutrition, Department of Animal Husbandry, University
More informationSTANDARD NORMALS AND NORMAL RANGES IN HEMATOLOGY*!
STANDARD NORMALS AND NORMAL RANGES IN HEMATOLOGY*! FRED.BOERNER From the Laboratories of the Graduate School of Medicine of the University of Pennsylvania. Philadelphia, Pennsylvania The routine blood
More informationCanadian Journal of Biochemistry and Physiology
Canadian Journal of Biochemistry and Physiology Issued by THE NATIONAL RESEARCH COUNCIL OF CANADA VOI,UME 37 AUGUST 1959 NUMBER 8 A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION1 Abstract Lipid
More informationand the mobilities of charged particles of varying
THE METHOD OF ELECTRICAL CONDUCTIVITY IN STUDIES ON BACTERIAL METABOLISM' JAMES B. ALLISON, JOHN A. ANDERSON, AND WILLIAM H. COLE Bureau of Biological Research, Rutgers University2 Received for publication
More informationSUGAR IN BACTERIAL CULTURES' of the media, gas or acid production are the usual criteria taken
A RAPID METHOD FOR THE DETERMINATION OF SUGAR IN BACTERIAL CULTURES' H. R. STILES, W. H. PETERSON AND E. B. FRED From the Departments of Agricultural Chemistry and Agricultural Bacteriology, University
More informationFitzgerald and Schmidt (1912) and Sears (1916, 1917) have demonstrated that bacteria can produce creatinine and the latter
PRECURSORS TO THE FORMATION OF CREATININE BY BACTERIA C. H. FISH AND T. D. BECKWITH Department of Bacteriology, University of California at Los Angeles Received for publication July 29, 1938 Creatinimne
More informationRedacted for Privacy
THESIS on COMPOSITION OF THE HOP (HUMULUS LUPUS) witg REFERENCE TO ITS PLANT FOOD CONTENT. Submitted to the Faculty of the AGRICULTURAL GOLLEGE for the degree of Bachelor of Science. by Redacted for Privacy
More informationESCHERICHIA COLI-MUTABILE1. antiseptics employed "activated" the lactase which was present, "activate" the lactase.
ON THE "ACTIVATION" OF THE LACTASE OF ESCHERICHIA COLI-MUTABILE1 CHARLES J. DEERE Department of Chemistry, University of Tennessee School of Biological Sciences, Memphis Received for publication August
More informationconductivity after its precipitation indicated that salts had been held freezing point or conductivity than the precipitation of the same
THE EFFECT ON THE MOLECULAR CONCENTRATION AND ELECTRICAL CONDUCTIVITY OF MUSCLE EXTRACTS OF REMOVAL OF THE PROTEIDS. BY G. N. STEWART, Western Reserve University, Cleveland, U.S.A. (Preliminary Note.)
More informationTHE EXCRETION OF BORIC ACID FROM THE HUMAN BODY.
THE EXCRETION OF BORIC ACID FROM THE HUMAN BODY. BY HARVEY W. WILEY, M.D. (From the Bureau of Chemistry, Washington, D. C.) (Received for publication, December 15, 1906.) In the studies which I have inaugurated
More informationNON-PROTEIN NITROGEN. (Bainbridge, 1911, and Sperry and Rettger, 1915) that bacteria. die from inanition in a medium which contains egg albumin,
BACTERIAL NUTRITION: FURTHER STUDIES ON THE UTILIZATION OF PROTEIN AND NON-PROTEIN NITROGEN NATHAN BERMAN AND LEO F. RETTGER From the Sheffield Laboratory of Bacteriology and Hygiene, Yale University Received
More informationPiS! * THE UNIVERSITY OF ILLINOIS *"%, LIBRARY
PiS! * THE UNIVERSITY *"%, OF ILLINOIS LIBRARY?t Ji. WON CIRCULATING CHECK FOR UNBOUND CIRCULATING COPY UNIVERSITY OF ILLINOIS Agricultural Experiment Station BULLETIN No. 159 BALANCED VS. UNBALANCED
More informationM6ller, McIntosh and Van Slyke (5) has been employed. The cases. changes in functional activity. Indications suggesting that such changes
STUDIES OF UREA EXCRETION. VIII. THE EFFECTS ON THE UREA CLEARANCE OF CHANGES IN PROTEIN AND SALT CONTENTS OF THE DIET BY CUTHBERT L. COPE I (From the Hospital of the Rockefeller Institute for Medical
More informationON THE COMPOSITION OF URINARY ALBUMIN.* BY FLORENTIN MEDIGRECEANU.
ON THE COMPOSITION OF URINARY ALBUMIN.* BY FLORENTIN MEDIGRECEANU. (From the Hospital of the Rockefeller Institute for Medical Research, New York.) Since the studies of Brown-Sequard, Teissier, L. Brunton,
More informationnitrogen-containiing groups gives increased solubility, but the
THE BACTERIOLOGICAL EVALUATION OF SOME NEW WATER SOLUBLE ORGANO-MERCURY COMPOUNDS, JOHN H. WALDO, H. A. SHONLE AND H. M. POWELL Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana
More informationDETERMINATION OF CARBOHYDRATES IN BACTERI-
DETERMINATION OF CARBOHYDRATES IN BACTERI- OLOGICAL CULTURE MEDIA CLARENCE F. SCHMIDT, JR. Department of Bacteriology, School of Medicine and Dentistry, University of Rochester, Rochester, New York Received
More informationCOLORIMETRIC DETERMINATION OF URIC ACID.
COLORIMETRIC DETERMINATION OF URIC ACID. ESTIMATION OF 0.03 TO 0.5 MG. QUANTITIES BY A NEW METHOD. BY J. LUCIEN MORRIS AND A. GARRARD MACLEOD. (From the Biochemistry Laboratory of the School of Medicine,
More informationBLOOD DENSITY IN GUINEA PIG ANAPHYLAXIS AND IN HAY FEVER ARTIFICIALLY INDUCED*
BLOOD DENSITY IN GUINEA PIG ANAPHYLAXIS AND IN HAY FEVER ARTIFICIALLY INDUCED* J. H. BLACK AND HARDY A. KEMP From the Department of Bacteriology and Preventive Medicine, Baylor University School of Medicine,
More informationJORIND 9(2) December, ISSN
SEQUENTIAL EXTRACTION OF Cu, Cd, Pb AND Zn FROM SOIL AROUND INDUSTRIAL WASTE DUMP SITES IN KADUNA ENVIRON USING SIMPLE AND SEQUENTIAL PROCEDURES. H.A Zakari, D.D. Adams, M Shimbayev, P. Nyam Department
More informationRECENT experiments (Prentice, 1933) 1
The Balance of Laying Pullets A. J. MACDONALD National Institute of Poultry Husbandry, Newport, Shropshire, England RECENT experiments (Prentice, 19) 1 concerning the protein requirements of laying pullets
More informationG/LITRE 5.0 g KOH g 0.5 g 0.05 g 0.01 g MgS047H20 NaCl CaCl2
A P P E N D IX -V III COMPOSITION OF USED MEDIA AND CHEMICAL REAGENTS 1. NITROGEN FREE BROMOTHYMOL BLUE (NFB) MEDIUM Dobereiner et al (1976) Same media was also used to check the effect of temperature
More informationII. THE EFFECT OF THE INGESTION OF GLYCINE ON THE EXCRETION OF ENDOGENOUS URIC ACID.
PURINE METABOLISM. II. THE EFFECT OF THE INGESTION OF GLYCINE ON THE EXCRETION OF ENDOGENOUS URIC ACID. BY A. A. CHRISTMAN AND E. C. MOSIER. (From the Laboratory of Physiological Chemistry, Medical School,
More informationOGY. IV. THE METABOLISM OF IODINE IN
RADIOACTIVE IODINE AS AN INDICATOR IN THYROID PHYSIOL- OGY IV THE METABOLISM OF IODINE IN GRAVES' 1 By S HERTZ, A ROBERTS, AND W T SALTER (From the Thyroid Clinic of the Massachusetts General Hospital,
More informationsuspended in it. The shortest killing times were: plant, was used to study its effects on a number of microorganisms
EFFECTS OF PANCREATIC ENZYMES ON THE TUBERCLE BACILLUS W. N. BERG Berg Biological Laboratory, New York City Received for publication, February 8, 1932 The literature contains confusing statements in regard
More informationNISIN. SYNONYMS INS No. 234 DEFINITION
SYNONYMS INS No. 234 NISIN Prepared at the 12th JECFA (1968), published in NMRS 45A (1969) and in FNP 52 (1992). Metals and arsenic specifications revised at the 63rd JECFA (2004). An ADI 0-33,000 units
More informationBalancing Rations for Sheep and Goats
Cooperative Extension Program University of Arkansas at Pine Bluff Animal Science FSA9613 Balancing Rations for Sheep and Goats David Fernandez Extension Livestock Specialist To achieve the best production
More informationTEMPERATURE ACTIVATION OF THE UREASE-UREA. inactivation temperature of the enzyme. For a specific enzyme
TEMPERATURE ACTIVATION OF THE UREASE-UREA SYSTEM USING UREASE OF PROTEUS VULGARIS' IRWIN W. SIZER With the technical assistance of ALFRED A. TYTELL Laboratory of Physiology and Biochemistry, Massachusetts
More informationACT Aspire. Science Test Prep
ACT Aspire Science Test Prep What do you need? What do you need? A Brain & An Elementary Education What do you NOT need? What do you NOT need? To be a genius! What does say about their own test? The ACT
More informationQuantitative measurements of zeta-potentials have been made. (Joffe, Hitchcock and Mudd, 1933). A most striking observation
VARIATIONS IN THE ELECTROPHORETIC MOBILITIES OF THE BRUCELLA GROUPS DOROTHEA E. SMITH AND ELEANORE W. JOFFE Department of Bacteriology, School of Medicine, University of Pennsylvania, Philadelphia, Pa.
More informationEFFECT OF THE AMINO ACIDS AND DIALYZABLE CONSTITUENTS OF EMBRYONIC TISSUE JUICE ON THE GROWTH OF FIBROBLASTS.
Published Online: 1 September, 1926 Supp Info: http://doi.org/10.1084/jem.44.3.397 Downloaded from jem.rupress.org on May 11, 2018 EFFECT OF THE AMINO ACIDS AND DIALYZABLE CONSTITUENTS OF EMBRYONIC TISSUE
More informationTHAT SEMINAL FLUID has functions in the process of conception other than
Influence of Seminal Fluid on Sperm Motility William Marden and N. T. Werthessen, Ph.D. THAT SEMINAL FLUID has functions in the process of conception other than to serve as a mere transport medium for
More informationAppendix 3 Media and solutions
Appendix 3 Media and solutions 1. Buffer solution Phosphate buffered saline (PBS) Recipe to prepare five litres of PBS Reagents Sodium chloride NaCl 40.0g Potassium chloride KCl 1.0g Potassium dihydrogen
More informationPLASMA AND URINARY AMINO-ACIDS IN DIABETES
PLASMA AND URINARY AMINOACIDS IN DIABETES By C. H. GRAY and ELIZABETH K. B. ILLING From the Department of Chemical Pathology, King's College Hospital, Denmark Hill, London, S.E. 5 (Received 22 June 1951)
More informationBY FRANCIS P. CHINARD WITH THE TECHNICAL ASSISTANCE OF DORA M. NEWELL. (Received for publication, July 28, 1948)
USE OF THE HYPOBROMITE REACTION FOR THE ESTIMATION OF AMMONIA PLUS UREA NITROGEN IN URINES CON- TAINING LARGE AMOUNTS OF PROTEIN; THE REAC- TION OF ALKALINE HYPOBROMITE WITH PROTEINS BY FRANCIS P. CHINARD
More informationStorage of waters, underground, surface, sea and sewage, phenomenon is the more puzzling because the waters in their
RELATION BETWEEN FOOD CONCENTRATION AND SURFACE FOR BACTERIAL GROWTH1 H. HEUKELEKIAN2 AND A. HELLER3 Agricultural Experiment Station, New Brunswick, New Jersey Received for publication April 19, 1940 Storage
More informationCXXXV. NEW SOURCES OF UREASE FOR DETERMINATION OF UREA
CXXXV. NEW SOURCES OF UREASE FOR DETERMINATION OF UREA BY MANAYATH DAMODARAN AND PALGHAT MANUVADHYAR SIVARAMAKRISHNAN From the University Biochemical Laboratory, Chepauk, Madras (Received 24 May 1937)
More informationOILSEED MEALS AND THE EFFECT OF PROCESSING ON THEIR SUBSEQUENT QUALITY JERRY C. WEIGEL SUMMARY INTRODUCTION
317 OLSEED MEALS AND THE EFFECT OF PROCESSNG ON THER SUBSEQUENT QUALTY JERRY C WEGEL SUMMARY Soybean protein product quality is a function of soybean quality and processing conditions Numerous factors
More informationTHE OCCURRENCE OF AMINO ACIDS AND OTHER OR- GANIC NITROGEN COMPOUNDS IN LAKE WATER.*
THE OCCURRENCE OF AMINO ACIDS AND OTHER OR- GANIC NITROGEN COMPOUNDS IN LAKE WATER.* BY W. H. PETERSON, E. B. FRED, AND B. P. DOMOGALLA. (From the Wisconsin Geological and Natural History Survey and the
More informationORGANIC MATTER' organic nutrients upon the growth of microorganisms, most of the experiments
BACTERIAL UTILIZATION OF LOW CONCENTRATIONS OF ORGANIC MATTER' CLAUDE E. ZOBELL AND CARROLL W. GRANT2 Scripps Institution of Oceanography, University of California, La Jolla Received for publication January
More informationdisturbance. mellitus, are known to result in, or be associated with, a disturbance study has, recently, been made (1). In a comprehensive study,
BLOOD SUGAR TIME CURVES BY I. M. RABINOWITCH (From the Department of Metabolism, Montreal General Hospital, Montreal, Canada) (Received for publication May 2, 926) Blood sugar time curves, or what are
More informationMETABOLISM AS RELATED TO CHROMOSOME STRUCTURE AND THE DURATION OF LIFE
Published Online: 20 March, 1931 Supp Info: http://doi.org/10.1085/jgp.14.4.463 Downloaded from jgp.rupress.org on November 7, 2018 METABOLISM AS RELATED TO CHROMOSOME STRUCTURE AND THE DURATION OF LIFE
More informationAN NEXURE. B log Sodium chloride 5g Distilled water (DW) 1 Litre ph: ] g 100 ml pl-l: g Glucose
AN NEXURE A. Composition of bacteriological media l. Alkaline Water (APW) B log Sodium chloride 5g Distilled water () 1 Litre ph: 9.110.] 2. Brilliant Green Bile Broth (BGLB ) Bile salt Brilliant green
More information104 Trop Anim Prod :2
04 Trop Anim Prod 977 : SUGAR CANE FOR BEEF PRODUCTION: DERINDED SUGAR CANE AND CHOPPED CANE COMPARED WITH HAY AND CITRUS PULP F M Dixon Bodles Agricultural Station Old Harbour, Jamaica In two separate
More informationThe Influence of Plankton on the Phosphate Content of Stored Sea-Water.
[ 1057 ] The Influence of Plankton on the Phosphate Content of Stored Sea-Water.,By By Robert Gill, M.Sc., Biochemist, Dove Marine Laboratory, Oullercoats. With 2 Figures in the Text. THE possibility of
More informationSurvival of Aerobic and Anaerobic Bacteria in
APPLIED MICROBIOLOGY, Mar. 1968, p. 445-449 Copyright 1968 American Society for Microbiology Vol. 16, No. 3 Printed in U.S.A. Survival of Aerobic and Anaerobic Bacteria in Chicken Meat During Freeze-Dehydration,
More informationBarley and Fat for Corn
Barley and Fat for Corn William G. Brown George H. Arscott Agricultural Experiment Station Oregon State College Corvallis Circular of Information 585 November 1957 S^U^uc «* * - Bar ey a C f,,, or Corn
More informationWright (1933) expressed the opinion that the beneficial effect of blood on. Bacteriology, The University of Texas, Austin, Texas
AN ANTIBRUCELLA FACTOR IN PEPTONES V. T. SCHUHARDT, L. J. RODE, J. W. FOSTER, AND GLENDA OGLESBY The Brucellosi8 Research Laboratory of the Clayton Foundation and the Department of Bacteriology, The University
More informationArchival copy: for current recommendations see or your local extension office.
NAME ADDRESS CLUB 4-H HORSE PROGRAM HORSE SCIENCE This educational material has been prepared for 4-H use by the Cooperative Extension Services of the U.S. Department of Agriculture and State Land-Grant
More informationTHE OHIO JOURNAL OF SCIENCE
THE OHIO JOURNAL OF SCIENCE VOL. XL MAY, 1940 No. 3 THE CARBON AND NITROGEN METABOLISM OP STEREUM GAUSAPATUM FRIES 1 J. ARTHUR HERRICK Kent State University The importance of Stereum gausapatum Fries as
More informationThe Utilization of Food Elements by Growing Chicks. V. A Comparison of Cottonseed Meal and Linseed Oil Meal as Portions of the Protein Concentrate
University of Nebraska - Lincoln DigitalCommons@University of Nebraska - Lincoln Historical Research Bulletins of the Nebraska Agricultural Experiment Station (1913-1993) Agricultural Research Division
More informationPLASMA IN VITRO 1 2. sodium citrate in 0.85 per cent sodium chloride solution.
STUDIES IN BLOOD COAGULATION: THE COAGULATION PROPERTIES OF CERTAIN GLOBULIN FRACTIONS OF NORMAL HUMAN PLASMA IN VITRO 1 2 By F. H. L. TAYLOR, C. S. DAVIDSON, H. J. TAGNON, M. A. ADAMS, A. H. MAcDONALD,
More informationReview for Final Exam Math 20
Review for Final Exam Math 20 Write the number in words. 1) 135,060 1) Rewrite the following number using digits. 2) Eight thousand, one hundred sixty-seven 2) Fill in the digits for the given place values
More informationUnderstanding a Soil Report
Understanding a Soil Report AGRONOMY SOIL ANALYSIS 1. Soil ph Soil ph is a measure of the acidity in the soil. An acidic soil has a greater amount of hydrogen (H+) ions and a ph below 7.0. Values above
More informationNUTRITIONAL CHARACTERISTICS OF THE TWO FERMENTED FISH PRODUCTS - HENTAK AND NGARI OF MANIPUR. CH. SAROJNALINI AND W. VISHWANATH
Journal of the Indian Fisheries Association 25, 1995 (Proc.Nat.Symp. Aquacrops), 75-81 NUTRITIONAL CHARACTERISTICS OF THE TWO FERMENTED FISH PRODUCTS - HENTAK AND NGARI OF MANIPUR. 75 CH. SAROJNALINI AND
More informationPatients studied and experimental procedures followed The patients studied in our experiments were 3 nephrotic
THE RELATION OF HIGH AND LOW UREA CLEARANCES TO THE INULIN AND CREATININE CLEARANCES IN CHIL- DREN WITH THE NEPHROTIC SYNDROME BY KENDALL EMERSON, JR., PALMER H. FUTCHER, AND LEE E. FARR (From the Hospital
More informationXLI. OXIDATION OF ALIPHATIC AMINES BY BRAIN AND OTHER TISSUES
XLI. OXIDATION OF ALIPHATIC AMINES BY BRAIN AND OTHER TISSUES BY CAECILIA ELISABETH MARY PUGH AND JUDA HIRSCH QUASTEL From the Biochemical Laboratory, Cardiff City Mental Hospital (Received 22 December
More informationRECALL INDEX Q1 2018
RECALL INDEX Recall Index Consumer product recalls decreased by just 3% to 72 marking two consecutive quarters with more than 70 recalls for the first time since. Recalled units decreased 92% to about
More informationTHE WATER AND ELECTROLYTE EXCHANGE OF NEREIS DIVERSICOLOR (MULLER)
34 THE WATER AND ELECTROLYTE EXCHANGE OF NEREIS DIVERSICOLOR (MULLER) BY W. G. ELLIS Zoology Department, University College of North Wales, Bangor {Received g December 1936) (With Nine Text-figures) IT
More informationHow would you prepare 455 grams of an aqueous solution that is 6.50% sodium sulfate by mass?
62 How would you prepare 455 grams of an aqueous solution that is 6.50% sodium sulfate by mass? Start a concentration calculation by writing the definition of the unit(s) you're using! We know everything
More informationEC252 Revised 1951 Practical Explanation of Some Terms in Animal Nutrition
University of Nebraska - Lincoln DigitalCommons@University of Nebraska - Lincoln Historical Materials from University of Nebraska- Lincoln Extension Extension 2-1951 EC252 Revised 1951 Practical Explanation
More informationBacteria & Viruses. Biology Science Department
Bacteria & Viruses What do you already know? What are the differences between viruses and bacteria? Are all bacteria harmful? When you get a cold, should you take an antibiotic to help you get better?
More informationEFFECT OF CARBON SOURCES ON FORMATION OF a-amylase AND GLUCOAMYLASE BY
J. Gen. App!. Microbiol,, 21, 51-59 (1975) EFFECT OF CARBON SOURCES ON FORMATION OF a-amylase AND GLUCOAMYLASE BY CLOSTRIDIUM ACETOBUTYLICUM BURT ENSLEY, JOHN J. McHUGH, AND LARRY L. BARTON Department
More informationDETERMINATION OF PHOSPHATE IN WATER AFTER STORAGE IN POLYETHYLENE1. J. Heron
DETERMINATION OF PHOSPHATE IN WATER AFTER STORAGE IN POLYETHYLENE1 J. Heron Freshwater Biological Association, Ambleside, England ABSTRACT The changes in phosphate concentration which take place when lake
More informationSOCIETY OF AMERICAN BACTERIOLOGISTS
PROCEEDINGS OF LOCAL BRANCHES OF THE SOCIETY OF AMERICAN BACTERIOLOGISTS CENTRAL NEW YORK STATE BRANCH 33RD SEMI-ANNUAL MEETING, GENEVA, NEW YORK, JUNE 5, 1937 INFLUENCE OF DRYING UPON SOIL USED AS A MEDIUM
More informationCATION-EXCHANGE CHARACTERISTICS OF WHEAT, BARLEY AND PEA DEPENDING ON THE OSMOTIC PRESSURE IN NUTRIENT SOLUTIONS OF LOW ph
BULG. J. PLANT PHYSIOL., 2002, 28(3 4), 35 45 35 CATION-EXCHANGE CHARACTERISTICS OF WHEAT, BARLEY AND PEA DEPENDING ON THE OSMOTIC PRESSURE IN NUTRIENT SOLUTIONS OF LOW ph Antoaneta Arsova * N. Pushkarov
More informationHYDRATION. that malnutrition or protein starvation, is the direct cause of the protein deficiency and that it affects only the albumin fraction of the
THE PLASMA PROTEINS IN RELATION TO BLOOD HYDRATION IV. MALNUTRITION AND THE SERUM PROTEINS By F. S. BRUCKMAN, L. M. D'ESOPO1 Aim J. P. PETERS (From the Department of Internal Medicine of Yale University
More information