Anti-aging / Soothing BEAUTY OF THE FUTURE

Transcription

1 Anti-aging / Soothing BEAUTY OF THE FUTURE

2 Anti-aging / Soothing

3 NATURAL INGREDIENT NATIVE TO THE INDIAN SUBCONTINENT USED FOR ITS SOOTHING AND ANTI-AGING PROPERTIES. FOCUS ON THE PRODUCT SOOTHING ACTIVITY COLLAMUNG protects the skin from the inflammation induced by UV. COLLAMUNG is an extract of Phaseolus sterols and flavonoids. From old time, the fermented beans extract has been used for dermatitis and wound healing. Phaseolus radiatus is used in cosmetic field for its nourishing properties. We are able to produce by a cold fermentation process a low molecular product that shows strong soothing and antiaging properties. TNF-α Gene expression (%) Radiatus seed, a mung bean rich in tannin, No UV UV COLLAMUNG COLLAMUNG COLLAMUNG COLLAMUNG OIL,1% OIL,5% OIL,1% OIL,5% Bisabolol,5% Human Keratinocytes were submitted to UV to promote inflammation. COLLAMUNG is proposed in 2 forms: Cells were treated with several concentrations of COLLAMUNG Oil or Bisabolol as positive controle. oil and water soluble. The expression of TNF-α gene was measured by qpcr.

4 BIOLOGICAL ACTIVITY COLLAMUNG promotes skin soothing effect by inhibiting the major inflammatory mediators : TNFα, COX2, IL1α. K E R AT I N O C Y T E S IL1α COX COLLAMUNG OIL,5 % IL1α Gene expression (%) Gene expression (%) FIBROBLASTS TNF α TNF α COX COLLAMUNG OIL 2ppm Human Keratinocytes and fibroblasts were incubated with to promote inflammation. Cells were treated with several concentrations of COLLAMUNG OIL. The expression of TNFα, COX2 and IL1α genes were measured by qpcr. COLLAMUNG reduces the expression of TNFα, COX2, IL1α, at gene and protein levels. D-panthenol 12 Protein synthesis (%) Control TNF-α COX ppm COLLAMUNG WATER SOLUBLE ppm 5 ppm TNF - α IL1- α 6 COX IL1- α 2 D-panthenol 5ppm COLLAMUNG WATER SOLUBLE ppm COLLAMUNG WATER SOLUBLE 5ppm GAPDH Human Keratinocytes were treated with several concentrations of COLLAMUNG WATER SOLUBLE or D-panthenol as positive control. The expression of TNFα, COX2 and IL1α proteins were measured by Western Blot analysis.

5 STRENGTHENING OF THE EPIDERMIS BARRIER Skin explants were treated 1 days with COLLAMUNG OIL. Thickening of the Stratum corneum after COLLAMUNG OIl treatment (Red arrow) The compartiments of the skin were observed by Immunohistochemistry. Day Day 1 COLLAMUNG OIL at 3% Day 1 Collagen 1 gene expression (%) Protects the skin and maintains the water balance UV COLLAMUNG OIL,5% Human Fibroblasts were submitted to UV to affect the Collagen I expression. Cells were treated with several concentrations of COLLAMUNG OIL. The expression of the Collagen I gene was measured by qpcr. IMPROVEMENT OF THE DERMIS In aged skin the dermal papillae tend to flatten. Skin explants were treated 1 days with COLLAMUNG OIL. UNTREATED Day UNTREATED Day 1 COLLAMUNG OIL 3% Day 1 The Collagen 1 was labeled by immunofluorescence (green). The dermal papillae are indicated by white arrows. COLLAMUNG OIL restores the dermis papillary and increases the Collagen I synthesis.

6 TECHNICAL INFORMATION INCI NAMES > OIL WATER SOLUBLE Caprylic/Capric Triglyceride, Phaseolus radiatus seed extract, Phenoxyethanol, Ethylhexylglycerin Phaseolus radiatus seed extract, Butylene Glycol, Phenoxyethanol, Ethylhexylglycerin APPLICATIONS Skin care Sun care APPLICATIONS DOSE OF USE > 1-5% 1-5% Oil > Water soluble > Oil > Yellowish green Water soluble > Amber FOR COSMETIC USE ONLY. ASPECT > SOLUBILITY > Oil soluble > Water soluble > ODOR > Characteristic odor

7 Factory address 82, Route du Guel 2413 PRIGONRIEUX France Tél. +33 () Fax : +33 () E.mail : info@sollicebiotech.com DESIGN YELLOW AGENCY () REV 215 MAY Head office CS BERGERAC Cedex France