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1 According to Regulation (EC) No. 1272/2008 Language : English Safety Data Sheet 1: Identification of substance / mixture 1. Product Identifier Product Name Product Codes CAS Number Other Names IUPAC MFCD Number EC/EINECS REACH Number GlykoPrep Digestion (RX) Cartridges WS0253, WS0283 NA NA NA NA NA Substance 2. Relevant identified uses of the substance or mixture and uses advised against Research and Development - Analytical chemistry 3. Details of the supplier of the safety data sheet ProZyme, Inc 3832 Bay Center Place, Hayward CA USA 4. Emergency telephone number +1 (760) Hazards Identification 1. Classification of the substance or mixture Non Hazardous 2. Label elements Non Hazardous Telephone: Fax: +1 (510) (510) info@prozyme.com Hazard Statements Non Hazardous Precautionary Phrases Non Hazardous 3. Other Hazards Non Hazardous 3. Composition / Information on Ingredients 1. Substances GlykoPrep RX Cartridges Assay:100% CAS Number :NA 2. Mixtures Not Relevant 4. First Aid Measures 1. Description of first aid measures Skin Contact Eye Contact Ingestion Inhalation Wash immediately with plenty of soap and water. Rinse with plenty of water. If symptoms arise, call a physician. Wash out mouth with water. Never give anything by mouth to an unconscious person. If symptoms arise, call a physician. Move to fresh air. If symptoms arise, consult a physician. 2. Most important symptoms and effects There may be mild irritation at the site of contact. Rev. AB Revision Date: 31 May 2013 Page 1 of 5

2 According to Regulation (EC) No. 1272/2008 Language : English Safety Data Sheet 3. Indication of any immediate medical attention Obtain medical attention in the event of any acute or delayed symptoms. 5. Firefighting measures 1. Extinguishing Media Suitable Unsuitable Suitable extinguishing media for surrounding fire should be used. NA 2. Special Hazards arising from the substance or mixture In combustion emits fumes of carbon oxides. 3. Advice for Fire Fighters Wear self-contained breathing apparatus for firefighting if necessary. Use personal protective equipment. 6. Accidental Release Measures 1. Personnal Precautions Refer to section 8 of SDS for personal protection details. 2. Environmental Precautions Do not discharge into drains or rivers. 3. Methods & Materials Clean contaminated floors and objects thoroughly while observing environmental regulations. 4. Preventing the occurence of secondary hazards. NA 7.Handling and Storage 1. Personnal Precautions Safe Handling Protection against explosions and fires Handle in accordance with all current regulations and standards. NA 2. Conditions for safe storage, including any incompatibilities Managing Storage Risks Store according to instructions in product labeling. Storage Controls Store according to product labeling. Maintaining Integrity Other advice Protect against physical damage. NA 3. Specific End Uses The end use(s) have not been fully determined. The substance is supplied for Research and Development purposes by professionals only. 8. Exposure Controls/Personal Protection 1. Control Parameters 2. Exposure Controls General protective and hydgiene measures Engineering measures Eye / Face Protection Hand protection Respiratory protection Skin protection Other personal protection advice Wear appropriate protective clothing (safety glasses, gloves, laboratory coat). The product does not contain any relevant quantities of materials with critical values that have to be monitored at the workplace. Safety Glasses with side-shields. Protective gloves. Respiratory protection not required under conditions of normal use. Wear appropriate protective clothing (safety glasses, gloves, laboratory coat). NA 9. Physical and Chemical Properties 1. Physical and Chemical Properties Page 2 of 5

3 According to Regulation (EC) No. 1272/2008 Language : English Appearance Odour Odour threshold PH Melting point / Freezing point Initial boiling point and boiling range Flash point Evaporation rate Flammability(solid,gas) Upper/lower flammability or explosive limits Vapour pressure Vapour density Relative density Solubility(ies): Partition coefficient: n-octanol/water Auto-ignition temperature Decomposition temperature Viscosity Explosive properties Oxidising properties Safety Data Sheet White powder in enclosed plastic housing 2. Other Information 10. Stability and Reactivity 1. Reactivity No dangerous reaction known under conditions of normal use. 2. Stability Stable under recommended storage conditions. 3. Possibility of Hazardous Reactions No hazardous reactions known. 4.Conditions to Avoid 5. Incompatible Materials Strong oxidizing agents and strong acids. 6. Hazardous Decomposition Products In combustion emits toxic fumes of carbon oxides. 11. Toxicology information 1. Information Acute Toxicity Skin corrosion/irritation Serious eye Damage/irritation Respiratory or skin sensitisation Germ Cell mutagenicity Carcinogenicity Reproductive toxicity STOT-single exposure STOT-repeated exposure Aspiration hazard 2. Additional To the best of our knowledge, the toxicological, carcinogenic and mutagenic properties have not been thoroughly investigated. Page 3 of 5

4 According to Regulation (EC) No. 1272/2008 Language : English 12. Ecological Information 1. Toxicity Safety Data Sheet 2. Persistance and degradability 3. Bio-Accumulative Potential 4. Mobility and Soil 5. Results of PBT & vpvb assessment 6. Other adverse effects 13. Disposal Considerations 1. Waste Treatment Methods Disposal Operations Should not be released into the environment. Prevent product from entering drains. Dispose of according to current regulations and standards. Disposal of Packaging 14. Transport Information Dispose of as unused product. Air (ICAO) 1. UN Number: 2. Shipping Name: None Hazardous 3. Transport hazard class(es): : Sub Class : 4. Packing group: 5. Environmental hazards: 6. Special Precautions for user: 7. Transport in bulk: Road (ADR) 1. UN Number: 2. Shipping Name: None Hazardous 3. Transport hazard class(es): : Sub Class : 4. Packing group: 5. Environmental hazards: 6. Special Precautions for user: 7. Transport in bulk: Sea (IMDG) 1. UN Number: 2. Shipping Name: None Hazardous 3. Transport hazard class(es): : Sub Class : Page 4 of 5

5 According to Regulation (EC) No. 1272/2008 Language : English Safety Data Sheet 4. Packing group: 5. Environmental hazards: 6. Special Precautions for user: 7. Transport in bulk: 15. Safety, health, environmental and national regulations 1. Safety, health, environmental and national regulations: 2. Safety Assessment No Chemical Safety Assessment. 16. Other Information 1. Other Information: ADR: Accord Europeen sur le transport des merchandises Dangereuses par Route(European Agreement concerning the International Carriage of Dangerous Goods by road); RID:Reglement International concernant le transport des merchandises dangereuses par chemin de fer (Regulations concerning the International transport of Dangerous Goods by Rail); IMDG: International Maritime Code for Dangerous Goods; IATA: International Air Transport Association; IATA-DGR: Dangerous Goods Regulations by the International Air Transport Association; ICAO:International Civil Aviation Organization; ICAO-TI: Technical Instructions by the ICAO; GHS: Globally Harmonized System of Classification and Labelling of Chemicals; CAS:Chemical Abstracts Service 2. Associated risk phrases according to european directive 67/548/EEC Non Hazardous 3. Disclaimer The above information is supplied in good faith and is believed to be correct. It does not claim to be all-inclusive and is intended to be used only as a guide. Final determination of the suitability of any material is the sole responsibility of the user. ProZyme shall not be held responsible for any damage resulting from handling or contact with the above product. This product is intended for in vitro research only. Page 5 of 5

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7 SAFETY DATA SHEET SECTION 1: Identification of the substance/mixture and of the company/undertaking 1.1. Product identifier Trade name or designation of the mixture Registration number Synonyms SDS number Product code Issue date Version number Revision date Supersedes date Denaturation Reagent - None. SDS WS0226 WS January-2013 AD 22-May January Relevant identified uses of the substance or mixture and uses advised against Identified uses Uses advised against Research and development. None known Details of the supplier of the safety data sheet Supplier Company name Address Telephone Contact person 1.4. Emergency telephone number ProZyme, Inc Bay Center Place, Hayward CA United States SECTION 2: Hazards identification 2.1. Classification of the substance or mixture Classification according to Directive 67/548/EEC or 1999/45/EC as amended Classification Xn;R22, Xi;R36/38 Classification according to Regulation (EC) No 1272/2008 as amended Health hazards Acute toxicity, oral Skin corrosion/irritation Serious eye damage/eye irritation Category 4 Category 2 Category 2 H302 - Harmful if swallowed. H315 - Causes skin irritation. H319 - Causes serious eye irritation. Hazard summary Physical hazards Not classified for physical hazards. Health hazards Harmful if swallowed. Irritating to eyes and skin. Environmental hazards Not classified for hazards to the environment. Specific hazards Ingestion may cause irritation and malaise. Main symptoms Irritation of eyes and mucous membranes. Skin irritation Label elements Label according to Regulation (EC) No. 1272/2008 as amended Contains: Guanidiniu m chloride Hazard pictograms Signal word Warning Revsion No.: AD Revision date: 22 May / 6

8 Hazard statements Precautionary statements Prevention Response Storage Disposal Supplemental label information H302 - Harmful if swallowed. H319 - Causes serious eye irritation. H315 - Causes skin irritation. P264 - Wash thoroughly after handling. P280 - Wear protective gloves and eye/face protection. P270 - Do not eat, drink or smoke when using this product. P301 + P312 - IF SWALLOWED: Call a POISON CENTRE or doctor/physician if you feel unwell. P330 - Rinse mouth. P305 + P351 + P338 - IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P337 + P313 - If eye irritation persists: Get medical advice/attention. P302 + P352 - IF ON SKIN: Wash with plenty of soap and water. If skin irritation occurs, seek medical advice/attention. Take off contaminated clothing and wash before re-use. Store away from incompatible materials. P501 - Dispose of contents/container in accordance with local/regional/national/international regulations Other hazards Not a PBT or vpvb substance or mixture. SECTION 3: Composition/information on ingredients 3.2. Mixtures General information Chemical name % CAS-No. / EC No. REACH Registration No. INDEX No. Notes Guanidinium chloride 50 - < 60 Classification: DSD: Xn;R22, Xi;R36/38 CLP: Acute Tox. 4;H302, Skin Irrit. 2;H315, Eye Irrit. 2;H Amino-2-(hydroxyme thyl) propane-1,3-diol hydrochloride Classification: DSD: Xi;R36/37/38 CLP: 3 - < Skin Irrit. 2;H315, Eye Irrit. 2;H319, STOT SE 3;H SECTION 4: First aid measures General information 4.1. Description of first aid measures Inhalation Skin contact Eye contact Ingestion 4.2. Most important symptoms and effects, both acute and delayed 4.3. Indication of any immediate medical attention and special treatment needed SECTION 5: Firefighting measures Ensure that medical personnel are aware of the material(s) involved, and take precautions to protect themselves. If symptomatic, move to fresh air. Get medical attention if symptoms occur. Immediately flush with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Wash contaminated clothing before reuse. Destroy or thoroughly clean contaminated shoes. Get medical attention if irritation develops and persists. Immediately flush with plenty of water for at least 15 minutes. If easy to do, remove contact lenses. Get medical attention if symptoms persist. Call a physician or poison control center immediately. Only induce vomiting at the instruction of medical personnel. Never give anything by mouth to an unconscious person. Exposed may experience eye tearing, redness, and discomfort. Treat symptomatically. General fire hazards 5.1. Extinguishing media Suitable extinguishing media This product is not flammable. Water. Water fog. Foam. Dry chemical powder. Carbon dioxide (CO2). 2 / 6

9 Unsuitable extinguishing media 5.2. Special hazards arising from the substance or mixture 5.3. Advice for firefighters Special protective equipment for firefighters Special fire fighting procedures None known. During fire, gases hazardous to health may be formed. Self-contained breathing apparatus and full protective clothing must be worn in case of fire. Use standard firefighting procedures and consider the hazards of other involved materials. SECTION 6: Accidental release measures 6.1. Personal precautions, protective equipment and emergency procedures For non-emergency personnel Avoid contact with skin and eyes. Wear appropriate personal protective equipment. For emergency responders Keep unnecessary personnel away. Use personal protection as recommended in section 8 of the SDS Environmental precautions Avoid discharge into drains, water courses or onto the ground Methods and material for containment and cleaning up 6.4. Reference to other sections SECTION 7: Handling and storage 7.1. Precautions for safe handling 7.2. Conditions for safe storage, including any incompatibilities 7.3. Specific end use(s) Reagent. SECTION 8: Exposure controls/personal protection Wipe up with absorbent material (e.g. cloth, fleece). Flush area with plenty of water. For personal protection, see section 8. For waste disposal, see section 13. Avoid contact with eyes, skin, and clothing. Do not taste or swallow. Wear appropriate personal protective equipment. Wash thoroughly after handling. Keep container closed. Store at 2-8 C (35-46 F). Store away from incompatible materials Control parameters Occupational exposure limits Biological limit values Recommended monitoring procedures Derived no-effect level (DNEL) Predicted no effect concentrations (PNECs) No exposure limits noted for ingredient(s). No biological exposure limits noted for the ingredient(s). Follow standard monitoring procedures Exposure controls Appropriate engineering controls No special requirements under ordinary conditions of use and with adequate ventilation. Individual protection measures, such as personal protective equipment General information Eye/face protection Skin protection Personal protective equipment should be chosen according to the CEN standards and in discussion with the supplier of the personal protective equipment. Wear safety glasses with side shields (or goggles). - Hand protection Use protective gloves. Suitable gloves can be recommended by the glove supplier. - Other Wear suitable protective clothing. Respiratory protection Thermal hazards Hygiene measures Environmental exposure controls Under normal conditions, respirator is not normally required. SECTION 9: Physical and chemical properties 9.1. Information on basic physical and chemical properties Appearance Physical state Handle in accordance with good industrial hygiene and safety practices. Environmental manager must be informed of all major releases. Liquid. 3 / 6

10 Odour Form Colour Odour threshold ph 8,8 Melting point/freezing point Initial boiling point and boiling range Flash point Evaporation rate Flammability (solid, gas) Liquid. Upper/lower flammability or explosive limits Flammability limit - lower (%) Flammability limit - upper (%) Vapour pressure Vapour density Relative density Solubility(ies) Partition coefficient (n-octanol/water) Decomposition temperature Viscosity Explosive properties Oxidizing properties 9.2. Other information No relevant additional information available. SECTION 10: Stability and reactivity Reactivity The product is non-reactive under normal conditions of use, storage and transport Chemical stability Material is stable under normal conditions Possibility of hazardous reactions Conditions to avoid Excessive heat. Hazardous polymerisation does not occur Incompatible materials Strong oxidising agents Hazardous decomposition products Carbon oxides. SECTION 11: Toxicological information General information Information on likely routes of exposure Ingestion Inhalation Skin contact Eye contact Symptoms Information on toxicological effects Acute toxicity Guanidinium chloride (CAS ) Acute Oral LD50 Skin corrosion/irritation Serious eye damage/irritation Occupational exposure to the substance or mixture may cause adverse effects. Harmful if swallowed. Under normal conditions of intended use, this material is not expected to be an inhalation hazard. Causes skin irritation. Causes serious eye irritation. Exposed may experience eye tearing, redness, and discomfort. Harmful if swallowed. Ingestion may cause irritation and malaise. Species Rat Causes skin irritation. Causes serious eye irritation. Test results 475 mg/kg 4 / 6

11 Respiratory sensitisation Skin sensitisation Germ cell mutagenicity Carcinogenicity Reproductive toxicity Specific target organ toxicity - single exposure Specific target organ toxicity - repeated exposure Aspiration hazard Mixture versus substance information Other information Not a skin sensitiser. SECTION 12: Ecological information Toxicity Not expected to be harmful to aquatic organisms Persistence and degradability Bioaccumulative potential Partition coefficient n-octanol/water (log Kow) Bioconcentration factor (BCF) Mobility in soil Results of PBT and vpvb assessment Other adverse effects None known. SECTION 13: Disposal considerations Waste treatment methods Residual waste Contaminated packaging EU waste code * Disposal methods/information SECTION 14: Transport information Not a PBT or vpvb substance or mixture. Dispose of in accordance with local regulations. Since emptied containers retain product residue, follow label warnings even after container is emptied. Dispose in accordance with all applicable regulations. ADR The product is not covered by international regulation on the transport of dangerous goods. RID The product is not covered by international regulation on the transport of dangerous goods. ADN The product is not covered by international regulation on the transport of dangerous goods. IATA The product is not covered by international regulation on the transport of dangerous goods. IMDG The product is not covered by international regulation on the transport of dangerous goods Transport in bulk according to Annex II of MARPOL 73/78 and the IBC Code SECTION 15: Regulatory information This substance/mixture is not intended to be transported in bulk Safety, health and environmental regulations/legislation specific for the substance or mixture EU regulations Regulation (EC) No. 1005/2009 on substances that deplete the ozone layer, Annex I Regulation (EC) No. 1005/2009 on substances that deplete the ozone layer, Annex II 5 / 6

12 Regulation (EC) No. 850/2004 On persistent organic pollutants, Annex I as amended Regulation (EC) No. 689/2008 concerning the export and import of dangerous chemicals, Annex I, part 1 as amended Regulation (EC) No. 689/2008 concerning the export and import of dangerous chemicals, Annex I, part 2 as amended Regulation (EC) No. 689/2008 concerning the export and import of dangerous chemicals, Annex I, part 3 as amended Regulation (EC) No. 689/2008 concerning the export and import of dangerous chemicals, Annex V as amended Regulation (EC) No. 166/2006 Annex II Pollutant Release and Transfer Registry Regulation (EC) No. 1907/2006, REACH Article 59(1) Candidate List as currently published by ECHA Authorisations Regulation (EC) No. 143/2011 Annex XIV Substances Subject to Authorisation Restrictions on use Regulation (EC) No. 1907/2006, REACH Annex XVII Substances subject to restriction on marketing and use as amended Directive 2004/37/EC: on the protection of workers from the risks related to exposure to carcinogens and mutagens at work Not regulated. Directive 92/85/EEC: on the safety and health of pregnant workers and workers who have recently given birth or are breastfeeding Not regulated. Other EU regulations Directive 96/82/EC (Seveso II) on the control of major-accident hazards involving dangerous substances Not regulated. Directive 98/24/EC on the protection of the health and safety of workers from the risks related to chemical agents at work Guanidinium chloride (CAS ) Directive 94/33/EC on the protection of young people at work Other regulations National regulations Chemical safety assessment SECTION 16: Other information The product is classified and labelled in accordance with Regulation (EC) 1272/2008 (CLP Regulation) as amended and respective national laws implementing EC directives. This Safety Data Sheet complies with the requirements of Regulation (EC) No 1907/2006. Follow national regulation for work with chemical agents. No Chemical Safety Assessment has been carried out. List of abbreviations References Information on evaluation method leading to the classification of mixture Full text of any statements or R-phrases and H-statements under Sections 2 to 15 Training information Disclaimer DNEL: Derived No-Effect Level. PNEC: Predicted No-Effect Concentration. PBT: Persistent, bioaccumulative and toxic. vpvb: Very Persistent and very Bioaccumulative. CLP: Regulation No. 1272/2008. DSD: Directive 67/548/EEC. HSDB - Hazardous Substances Data Bank Registry of Toxic Effects of Chemical Substances (RTECS) The mixture is classified based on test data for physical hazards. The classification for health and environmental hazards is derived by a combination of calculation methods and test data, if available. For details, refer to Sections 9, 11 and 12. R22 Harmful if swallowed. R36/37/38 Irritating to eyes, respiratory system and skin. R36/38 Irritating to eyes and skin. H302 - Harmful if swallowed. H315 - Causes skin irritation. H319 - Causes serious eye irritation. H335 - May cause respiratory irritation. Follow training instructions when handling this material. The information in the sheet was written based on the best knowledge and experience currently available. 6 / 6

13 SAFETY DATA SHEET SECTION 1: Identification of the substance/mixture and of the company/undertaking 1.1. Product identifier Trade name or designation of the mixture Registration number Synonyms SDS number Product code Issue date Version number Revision date Supersedes date Blocking Reagent - None. SDS WS0255 WS January-2013 AD 24-May January Relevant identified uses of the substance or mixture and uses advised against Identified uses Uses advised against Research and development. None known Details of the supplier of the safety data sheet Supplier Company name Address Telephone Contact person 1.4. Emergency telephone number ProZyme, Inc Bay Center Place, Hayward CA United States SECTION 2: Hazards identification 2.1. Classification of the substance or mixture Classification according to Directive 67/548/EEC or 1999/45/EC as amended This preparation does not meet the criteria for classification according to Directive 1999/45/EC as amended. Classification according to Regulation (EC) No 1272/2008 as amended This mixture does not meet the criteria for classification according to Regulation (EC) 1272/2008 as amended. Hazard summary Physical hazards Health hazards Environmental hazards Specific hazards Main symptoms 2.2. Label elements Not classified for physical hazards. Not classified for health hazards. Not classified for hazards to the environment. Prolonged or repeated contact may dry skin and cause irritation. Direct contact with eyes may cause temporary irritation. Irritant effects. Label according to Regulation (EC) No. 1272/2008 as amended Hazard pictograms Signal word Hazard statements Precautionary statements Prevention Response Storage Disposal None. None. The mixture does not meet the criteria for classification. Observe good industrial hygiene practices. Wash hands after handling. Supplemental label information Store away from incompatible materials. Dispose of waste and residues in accordance with local authority requirements. Revsion No.: AD Revision date: 24 May / 6

14 2.3. Other hazards Not a PBT or vpvb substance or mixture. SECTION 3: Composition/information on ingredients 3.2. Mixtures The components are not hazardous or are below required disclosure limits. SECTION 4: First aid measures General information 4.1. Description of first aid measures Inhalation Skin contact Eye contact Ingestion 4.2. Most important symptoms and effects, both acute and delayed 4.3. Indication of any immediate medical attention and special treatment needed Ensure that medical personnel are aware of the material(s) involved, and take precautions to protect themselves. If symptomatic, move to fresh air. Get medical attention if symptoms persist. Wash skin with soap and water. Get medical attention if irritation develops and persists. Rinse with plenty of water. Get medical attention if symptoms persist. If swallowed, rinse mouth with water (only if the person is conscious). Irritant effects. SECTION 5: Firefighting measures Treat symptomatically. General fire hazards 5.1. Extinguishing media Suitable extinguishing media Unsuitable extinguishing media 5.2. Special hazards arising from the substance or mixture 5.3. Advice for firefighters Special protective equipment for firefighters Special fire fighting procedures This product is not flammable. Water. Water fog. Foam. Dry chemical powder. Carbon dioxide (CO2). None known. During fire, gases hazardous to health may be formed. Self-contained breathing apparatus and full protective clothing must be worn in case of fire. Use standard firefighting procedures and consider the hazards of other involved materials. SECTION 6: Accidental release measures 6.1. Personal precautions, protective equipment and emergency procedures For non-emergency personnel Wear appropriate personal protective equipment. For emergency responders Keep unnecessary personnel away Environmental precautions Avoid discharge into drains, water courses or onto the ground Methods and material for containment and cleaning up 6.4. Reference to other sections SECTION 7: Handling and storage 7.1. Precautions for safe handling 7.2. Conditions for safe storage, including any incompatibilities 7.3. Specific end use(s) Reagent. SECTION 8: Exposure controls/personal protection Wipe up with absorbent material (e.g. cloth, fleece). Flush area with plenty of water. For personal protection, see section 8. For waste disposal, see section 13. Avoid breathing mist or vapour. Avoid contact with eyes, skin, and clothing. Wash thoroughly after handling. Use only with adequate ventilation. Store at 2-8 C (35-46 F). Store away from incompatible materials Control parameters Occupational exposure limits Biological limit values Recommended monitoring procedures No exposure limits noted for ingredient(s). No biological exposure limits noted for the ingredient(s). Follow standard monitoring procedures. 2 / 6

15 Derived no-effect level (DNEL) Predicted no effect concentrations (PNECs) 8.2. Exposure controls Appropriate engineering controls No special requirements under ordinary conditions of use and with adequate ventilation. Individual protection measures, such as personal protective equipment General information Eye/face protection Skin protection Personal protective equipment should be chosen according to the CEN standards and in discussion with the supplier of the personal protective equipment. Risk of contact: Wear safety glasses with side shields (or goggles). - Hand protection Risk of contact: Chemical resistant gloves are recommended. - Other Wear suitable protective clothing. Respiratory protection Thermal hazards Hygiene measures Environmental exposure controls Under normal conditions, respirator is not normally required. SECTION 9: Physical and chemical properties 9.1. Information on basic physical and chemical properties Appearance Odour Physical state Form Colour Odour threshold ph Melting point/freezing point Initial boiling point and boiling range Flash point Evaporation rate Flammability (solid, gas) Handle in accordance with good industrial hygiene and safety practices. Environmental manager must be informed of all major releases. Clear liquid. Liquid. Liquid. Colourless to light yellow. Upper/lower flammability or explosive limits Flammability limit - lower (%) Flammability limit - upper (%) Vapour pressure Vapour density Relative density Solubility(ies) Partition coefficient (n-octanol/water) Decomposition temperature Viscosity Explosive properties Oxidizing properties 9.2. Other information No relevant additional information available. SECTION 10: Stability and reactivity Reactivity The product is non-reactive under normal conditions of use, storage and transport Chemical stability Material is stable under normal conditions Possibility of hazardous reactions Hazardous polymerisation does not occur. 3 / 6

16 10.4. Conditions to avoid Excessive heat Incompatible materials Strong oxidising agents Hazardous decomposition products Carbon oxides. SECTION 11: Toxicological information General information Information on likely routes of exposure Ingestion Inhalation Skin contact Eye contact Symptoms Occupational exposure to the substance or mixture may cause adverse effects. No harmful effects expected in amounts likely to be ingested by accident. Under normal conditions of intended use, this material is not expected to be an inhalation hazard. Prolonged or repeated contact may dry skin and cause irritation. Direct contact with eyes may cause temporary irritation. Irritant effects Information on toxicological effects Acute toxicity Skin corrosion/irritation Serious eye damage/irritation Respiratory sensitisation Skin sensitisation Germ cell mutagenicity Carcinogenicity Reproductive toxicity Specific target organ toxicity - single exposure Specific target organ toxicity - repeated exposure Aspiration hazard Mixture versus substance information Other information May cause discomfort if swallowed. Prolonged or repeated contact may dry skin and cause irritation. Direct contact with eyes may cause temporary irritation. Not a skin sensitiser. SECTION 12: Ecological information Toxicity Not expected to be harmful to aquatic organisms Persistence and degradability Bioaccumulative potential Partition coefficient n-octanol/water (log Kow) Bioconcentration factor (BCF) Mobility in soil Results of PBT and vpvb assessment Other adverse effects None known. SECTION 13: Disposal considerations Waste treatment methods Residual waste Contaminated packaging EU waste code Disposal methods/information SECTION 14: Transport information Not a PBT or vpvb substance or mixture. Dispose of in accordance with local regulations. Since emptied containers retain product residue, follow label warnings even after container is emptied. Dispose in accordance with all applicable regulations. ADR The product is not covered by international regulation on the transport of dangerous goods. 4 / 6

17 RID The product is not covered by international regulation on the transport of dangerous goods. ADN The product is not covered by international regulation on the transport of dangerous goods. IATA The product is not covered by international regulation on the transport of dangerous goods. IMDG The product is not covered by international regulation on the transport of dangerous goods Transport in bulk according to Annex II of MARPOL 73/78 and the IBC Code SECTION 15: Regulatory information This substance/mixture is not intended to be transported in bulk Safety, health and environmental regulations/legislation specific for the substance or mixture EU regulations Regulation (EC) No. 1005/2009 on substances that deplete the ozone layer, Annex I Regulation (EC) No. 1005/2009 on substances that deplete the ozone layer, Annex II Regulation (EC) No. 850/2004 On persistent organic pollutants, Annex I as amended Regulation (EC) No. 689/2008 concerning the export and import of dangerous chemicals, Annex I, part 1 as amended Regulation (EC) No. 689/2008 concerning the export and import of dangerous chemicals, Annex I, part 2 as amended Regulation (EC) No. 689/2008 concerning the export and import of dangerous chemicals, Annex I, part 3 as amended Regulation (EC) No. 689/2008 concerning the export and import of dangerous chemicals, Annex V as amended Regulation (EC) No. 166/2006 Annex II Pollutant Release and Transfer Registry Regulation (EC) No. 1907/2006, REACH Article 59(1) Candidate List as currently published by ECHA Authorisations Regulation (EC) No. 143/2011 Annex XIV Substances Subject to Authorisation Restrictions on use Regulation (EC) No. 1907/2006, REACH Annex XVII Substances subject to restriction on marketing and use as amended Directive 2004/37/EC: on the protection of workers from the risks related to exposure to carcinogens and mutagens at work Not regulated. Directive 92/85/EEC: on the safety and health of pregnant workers and workers who have recently given birth or are breastfeeding Not regulated. Other EU regulations Directive 96/82/EC (Seveso II) on the control of major-accident hazards involving dangerous substances Not regulated. Directive 98/24/EC on the protection of the health and safety of workers from the risks related to chemical agents at work Directive 94/33/EC on the protection of young people at work Other regulations National regulations Chemical safety assessment This substance does not meet the criteria for classification according to Regulation (EC) 1272/2008 (CLP Regulation) and Directive 67/548/EEC and their amendments respectively. This Safety Data Sheet complies with the requirements of Regulation (EC) No 1907/2006. Follow national regulation for work with chemical agents. No Chemical Safety Assessment has been carried out. 5 / 6

18 SECTION 16: Other information List of abbreviations References DNEL: Derived No-Effect Level. PNEC: Predicted No-Effect Concentration. PBT: Persistent, bioaccumulative and toxic. vpvb: Very Persistent and very Bioaccumulative. CLP: Regulation No. 1272/2008. DSD: Directive 67/548/EEC. HSDB - Hazardous Substances Data Bank Registry of Toxic Effects of Chemical Substances (RTECS) Information on evaluation method leading to the classification of mixture Full text of any statements or R-phrases and H-statements under Sections 2 to 15 Training information Disclaimer The mixture is classified based on test data for physical hazards. The classification for health and environmental hazards is derived by a combination of calculation methods and test data, if available. For details, refer to Sections 9, 11 and 12. None. Follow training instructions when handling this material. The information in the sheet was written based on the best knowledge and experience currently available. 6 / 6

19 According to Regulation (EC) No. 1272/2008 Language : English Safety Data Sheet 1: Identification of substance / mixture 1. Product Identifier Product Name Product Code CAS Number Other Names IUPAC MFCD Number EC/EINECS REACH Number Finishing Reagent WS Mixture 2. Relevant identified uses of the substance or mixture and uses advised against Research and Development 3. Details of the supplier of the safety data sheet ProZyme, Inc 3832 Bay Center Place, Hayward CA USA 4. Emergency telephone number +1 (760) Hazards Identification 1. Classification of the substance or mixture Telephone: Fax: +1 (510) (510) info@prozyme.com H315 Skin Irrit. 2 R38, R36/38, R36/37/38, R37/38 H317 Skin Sens. 1 R43, R42/43 H319 Eye Irrit. 2 R36, R36/37, R36/38, R36/37/38 No Resource File 2. Label elements Signal Word Warning Hazard Statements H315 Causes skin irritation. H317 May cause an allergic skin reaction. H319 Causes serious eye irritation. Precautionary Phrases Non Hazardous 3. Other Hazards Additional precautionary phrases are located throughout the safety data sheet. 3. Composition / Information on Ingredients 1. Substances No Resource File 2. Mixtures Concentration Product Name CAS Number EC/EINECS >50% Water <10% Acetic Acid First Aid Measures Rev. AB Revision Date: 30 May 2013 Page 1 of 6

20 According to Regulation (EC) No. 1272/2008 Language : English 1. Description of first aid measures Skin Contact Safety Data Sheet Wash immediately with plenty of soap and water. Consult a doctor. Eye Contact Ingestion Inhalation P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If symptoms arise, consult a physician. Continue rinsing eyes during transport to hospital. Never give anything by mouth to an unconscious person. Rinse mouth with water. If symptoms arise, call a physician. Do not induce vomiting without medical advice. Move to fresh air. If symptoms arise, call a physician. If not breathing, give artificial respiration. 2. Most important symptoms and effects May cause serious eye or skin irritation. 3. Indication of any immediate medical attention 5. Firefighting measures 1. Extinguishing Media Suitable Use water spray, alcohol resistant foam, dry chemical powder or carbon dioxide. Unsuitable NA 2. Special Hazards arising from the substance or mixture In combustion emits toxic fumes of carbon dioxide / carbon monoxide. 3. Advice for Fire Fighters Wear self-contained breathing apparatus for firefighting if necessary. 6. Accidental Release Measures 1. Personnal Precautions Use personal protective equipment. Avoid breathing vapors, mist or gas. Ensure adequate ventilation. 2. Environmental Precautions Do not allow product to enter drains. 3. Methods & Materials Absorb small spills with inert absorbant material. Transfer to a suitable, closed container for disposal. 4. Preventing the occurence of secondary hazards. NA 7.Handling and Storage 1. Personnal Precautions Safe Handling Protection against explosions and fires 2. Conditions for safe storage, including any incompatibilities Managing Storage Risks Avoid direct contact with the substance. NA Store in original containers, tightly closed, in a dry and well-ventilated place. Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Storage Controls Maintaining Integrity Other advice NA NA NA 3. Specific End Uses The substance is supplied for Research and Development purposes by professionals only. Page 2 of 6

21 According to Regulation (EC) No. 1272/2008 Language : English Safety Data Sheet 8. Exposure Controls/Personal Protection 1. Control Parameters 2. Exposure Controls General protective and hydgiene measures Engineering measures Eye / Face Protection Hand protection Respiratory protection Skin protection Other personal protection advice P280: Wear protective gloves/protective clothing/eye protection/face protection. Ensure there is sufficient ventilation of the area. Safety Glasses with side-shields. Protective gloves. Gloves must be inspected prior to use. Use proper glover removal technique (without touching glove's outer surface) to avoid skin contact with this product. P261: Avoid breathing dust/fume/gas/mist/vapours/spray. Respiratory protection not required under conditions of normal use. Protective clothing (safety glasses, gloves, lab coat). NA 9. Physical and Chemical Properties 1. Physical and Chemical Properties Appearance Odour Odour threshold PH Melting point / Freezing point Initial boiling point and boiling range Flash point Evaporation rate Flammability(solid,gas) Upper/lower flammability or explosive limits Vapour pressure Vapour density Relative density Solubility(ies): Partition coefficient: n-octanol/water Auto-ignition temperature Decomposition temperature Viscosity Explosive properties Oxidising properties Clear liquid pungent 2. Other Information NA 10. Stability and Reactivity 1. Reactivity Stable under recommended storage conditions. 2. Stability Stable under recommended storage conditions. 3. Possibility of Hazardous Reactions Page 3 of 6

22 According to Regulation (EC) No. 1272/2008 Language : English Safety Data Sheet 4.Conditions to Avoid 5. Incompatible Materials Oxidizing agents, metals, soluble carbonates and phosphates, hydroxides, peroxides, permanganates (e.g. potassium permanganate) amines, alcohols 6. Hazardous Decomposition Products Hazardous decomposition products formed under fire conditions: carbon oxides 11. Toxicology information 1. Information Acute Toxicity Skin corrosion/irritation Serious eye Damage/irritation Respiratory or skin sensitisation Germ Cell mutagenicity May cause skin irritation. May cause eye irritation. Carcinogenicity Reproductive toxicity STOT-single exposure STOT-repeated exposure Aspiration hazard May be harmful if inhaled. 2. Additional 12. Ecological Information 1. Toxicity 2. Persistance and degradability 3. Bio-Accumulative Potential 4. Mobility and Soil 5. Results of PBT & vpvb assessment 6. Other adverse effects 13. Disposal Considerations 1. Waste Treatment Methods Page 4 of 6

23 According to Regulation (EC) No. 1272/2008 Language : English Disposal Operations Safety Data Sheet Contact a licensed professional wate disposal service to dispose of this material. Dispose of according to local regulations. Disposal of Packaging Dispose of as unused product. 14. Transport Information Air (ICAO) 1. UN Number: 2. Shipping Name: None Hazardous 3. Transport hazard class(es): : Sub Class : 4. Packing group: 5. Environmental hazards: 6. Special Precautions for user: 7. Transport in bulk: Road (ADR) 1. UN Number: 2. Shipping Name: None Hazardous 3. Transport hazard class(es): : Sub Class : 4. Packing group: 5. Environmental hazards: 6. Special Precautions for user: 7. Transport in bulk: Sea (IMDG) 1. UN Number: 2. Shipping Name: None Hazardous 3. Transport hazard class(es): : Sub Class : 4. Packing group: 5. Environmental hazards: 6. Special Precautions for user: 7. Transport in bulk: 15. Safety, health, environmental and national regulations 1. Safety, health, environmental and national regulations: OSHA hazards: Target Organ Effect, Corrosive SARA 302 : No chemicals in this material are subject to the reporting requirements of SARA title III<Section 302. SARA 313 : This material does not contain any chemical components with known CAS numbers that exceed the threshold (De Minimus) reporting levels establishes by SARA Title III, Section 313. SARA 311/312 Hazards: Acute Health Hazard, Chronic Health Hazard Massachusetts Right to Know : Acetic Acid CAS Number Pennsylvania Right to Know : Water, Acetic Acid CAS Number Page 5 of 6

24 According to Regulation (EC) No. 1272/2008 Language : English 2. Safety Assessment No Chemical Safety Assessment Safety Data Sheet 16. Other Information 1. Other Information: ADR: Accord Europeen sur le transport des merchandises Dangereuses par Route(European Agreement concerning the International Carriage of Dangerous Goods by road) RID: Reglement International concernant le transport des merchandises dangereuses par chemin de fer (Regulations concerning the International transport of Dangerous Goods by Rail) IMDG: International Maritime Code for Dangerous Goods IATA: International Air Transport Association IATA-DGR: Dangerous Goods Regulations by the International Air Transport Association ICAO: International Civil Aviation Organization ICAO-TI: Technical Instructions by the ICAO GHS: Globally Harmonized System of Classification and Labelling of Chemicals CAS: Chemical Abstracts Service 2. Associated risk phrases according to european directive 67/548/EEC R36 Irritating to eyes. R36/37 Irritating to eyes and respiratory system. R36/37/38 Irritating to eyes, respiratory system and skin. R36/38 Irritating to eyes and skin. R37/38 Irritating to respiratory system and skin. R38 Irritating to skin. R42/43 May cause sensitization by inhalation and skin contact. R43 May cause sensitisation by skin contact. 3. Disclaimer The above information is supplied in good faith and is believed to be correct. It does not claim to be all-inclusive and is intended to be used only as a guide. Final determination of the suitability of any material is the sole responsibility of the user. ProZyme shall not be held responsible for any damage resulting from handling or contact with the above product. This product is intended for in vitro research only. The above information is supplied in good faith and is believed to be correct. It does not claim to be all-inclusive and is intended to be used only as a guide. Final determination of the suitability of any material is the sole responsibility of the user. ProZyme shall not be held responsible for any damage resulting from handling or contact with the above product. This product is intended for in vitro research only. Page 6 of 6

25 SAFETY DATA SHEET SECTION 1: Identification of the substance/mixture and of the company/undertaking 1.1. Product identifier Trade name or designation of the mixture Registration number Synonyms SDS number Product code 25X Digestion Buffer - None. WS0276 WS0276 Issue date 24-January-2013 Version number AB Revision date Supersedes date 30-January November Relevant identified uses of the substance or mixture and uses advised against Identified uses Uses advised against Research and development. None known Details of the supplier of the safety data sheet Supplier Company name Address Telephone Contact person 1.4. Emergency telephone number ProZyme, Inc Bay Center Place, Hayward CA United States SECTION 2: Hazards identification 2.1. Classification of the substance or mixture Classification according to Directive 67/548/EEC or 1999/45/EC as amended This preparation does not meet the criteria for classification according to Directive 1999/45/EC as amended. Classification according to Regulation (EC) No 1272/2008 as amended This mixture does not meet the criteria for classification according to Regulation (EC) 1272/2008 as amended. Hazard summary Physical hazards Health hazards Environmental hazards Specific hazards Main symptoms 2.2. Label elements Not classified for physical hazards. Not classified for health hazards. Not classified for hazards to the environment. Prolonged or repeated contact may dry skin and cause irritation. Direct contact with eyes may cause temporary irritation. Irritant effects. Label according to Regulation (EC) No. 1272/2008 as amended Hazard pictograms Signal word Hazard statements Precautionary statements Prevention Response Storage Disposal None. None. The mixture does not meet the criteria for classification. Observe good industrial hygiene practices. Wash hands after handling. Supplemental label information Store away from incompatible materials. Dispose of waste and residues in accordance with local authority requirements. Revision No.: AB Revision date: 30 Jan / 6

26 2.3. Other hazards Not a PBT or vpvb substance or mixture. SECTION 3: Composition/information on ingredients 3.2. Mixtures General information Chemical name % CAS-No. / EC No. REACH Registration No. INDEX No. Notes Ammonium formate 1-5 Classification: DSD: Xi;R36/37/38 CLP: Skin Irrit. 2;H315, Eye Irrit. 2;H319, STOT SE 3;H SECTION 4: First aid measures General information 4.1. Description of first aid measures Inhalation Skin contact Eye contact Ingestion 4.2. Most important symptoms and effects, both acute and delayed 4.3. Indication of any immediate medical attention and special treatment needed Ensure that medical personnel are aware of the material(s) involved, and take precautions to protect themselves. If symptomatic, move to fresh air. Get medical attention if symptoms occur. Wash skin with soap and water. Get medical attention if irritation develops and persists. Immediately flush with plenty of water for at least 15 minutes. Get medical attention if symptoms persist. If swallowed, rinse mouth with water (only if the person is conscious). Irritant effects. SECTION 5: Firefighting measures Treat symptomatically. General fire hazards 5.1. Extinguishing media Suitable extinguishing media Unsuitable extinguishing media 5.2. Special hazards arising from the substance or mixture 5.3. Advice for firefighters Special protective equipment for firefighters Special fire fighting procedures This product is not flammable. Water. Water fog. Foam. Dry chemical powder. Carbon dioxide (CO2). None known. During fire, gases hazardous to health may be formed. Self-contained breathing apparatus and full protective clothing must be worn in case of fire. Use standard firefighting procedures and consider the hazards of other involved materials. SECTION 6: Accidental release measures 6.1. Personal precautions, protective equipment and emergency procedures For non-emergency personnel Avoid contact with skin and eyes. Wear appropriate personal protective equipment. For emergency responders Keep unnecessary personnel away. Use personal protection as recommended in section 8 of the SDS Environmental precautions Avoid discharge into drains, water courses or onto the ground Methods and material for containment and cleaning up 6.4. Reference to other sections SECTION 7: Handling and storage Wipe up with absorbent material (e.g. cloth, fleece). Flush area with plenty of water. For personal protection, see section 8. For waste disposal, see section Precautions for safe handling Avoid contact with eyes, skin, and clothing. Wear appropriate personal protective equipment. Wash thoroughly after handling. 2 / 6

27 7.2. Conditions for safe storage, including any incompatibilities 7.3. Specific end use(s) Reagent. SECTION 8: Exposure controls/personal protection Keep container closed. Store at 2-8 C (35-46 F). Store away from incompatible materials Control parameters Occupational exposure limits Biological limit values Recommended monitoring procedures Derived no-effect level (DNEL) Predicted no effect concentrations (PNECs) No exposure limits noted for ingredient(s). No biological exposure limits noted for the ingredient(s). Follow standard monitoring procedures Exposure controls Appropriate engineering controls No special requirements under ordinary conditions of use and with adequate ventilation. Individual protection measures, such as personal protective equipment General information Eye/face protection Skin protection Personal protective equipment should be chosen according to the CEN standards and in discussion with the supplier of the personal protective equipment. Wear safety glasses with side shields (or goggles). - Hand protection Use protective gloves. Suitable gloves can be recommended by the glove supplier. - Other Wear suitable protective clothing. Respiratory protection Thermal hazards Hygiene measures Environmental exposure controls Under normal conditions, respirator is not normally required. SECTION 9: Physical and chemical properties 9.1. Information on basic physical and chemical properties Appearance Odour Physical state Form Colour Odour threshold ph Melting point/freezing point Initial boiling point and boiling range Flash point Evaporation rate Flammability (solid, gas) Handle in accordance with good industrial hygiene and safety practices. Environmental manager must be informed of all major releases. Liquid. Liquid. Upper/lower flammability or explosive limits Flammability limit - lower (%) Flammability limit - upper (%) Vapour pressure Vapour density Relative density Solubility(ies) Partition coefficient (n-octanol/water) Decomposition temperature 3 / 6

28 Viscosity Explosive properties Oxidizing properties 9.2. Other information No relevant additional information available. SECTION 10: Stability and reactivity Reactivity The product is non-reactive under normal conditions of use, storage and transport Chemical stability Material is stable under normal conditions Possibility of hazardous reactions Conditions to avoid Excessive heat. Hazardous polymerisation does not occur Incompatible materials Strong oxidising agents Hazardous decomposition products Carbon oxides. Ammonia. SECTION 11: Toxicological information General information Information on likely routes of exposure Ingestion Inhalation Skin contact Eye contact Symptoms Occupational exposure to the substance or mixture may cause adverse effects. No harmful effects expected in amounts likely to be ingested by accident. Under normal conditions of intended use, this material is not expected to be an inhalation hazard. Prolonged or repeated contact may dry skin and cause irritation. Direct contact with eyes may cause temporary irritation. Irritant effects Information on toxicological effects Acute toxicity Skin corrosion/irritation Serious eye damage/irritation Respiratory sensitisation Skin sensitisation Germ cell mutagenicity Carcinogenicity Reproductive toxicity Specific target organ toxicity - single exposure Specific target organ toxicity - repeated exposure Aspiration hazard Mixture versus substance information Other information May cause discomfort if swallowed. Prolonged or repeated contact may dry skin and cause irritation. Direct contact with eyes may cause temporary irritation. Not a skin sensitiser. SECTION 12: Ecological information Toxicity Not expected to be harmful to aquatic organisms Persistence and degradability Bioaccumulative potential Partition coefficient n-octanol/water (log Kow) Bioconcentration factor (BCF) Mobility in soil Results of PBT and vpvb assessment Other adverse effects None known. SECTION 13: Disposal considerations Waste treatment methods Not a PBT or vpvb substance or mixture. 4 / 6

29 Residual waste Contaminated packaging EU waste code Disposal methods/information SECTION 14: Transport information Dispose of in accordance with local regulations. Since emptied containers retain product residue, follow label warnings even after container is emptied. Dispose in accordance with all applicable regulations. ADR The product is not covered by international regulation on the transport of dangerous goods. RID The product is not covered by international regulation on the transport of dangerous goods. ADN The product is not covered by international regulation on the transport of dangerous goods. IATA The product is not covered by international regulation on the transport of dangerous goods. IMDG The product is not covered by international regulation on the transport of dangerous goods Transport in bulk according to Annex II of MARPOL 73/78 and the IBC Code SECTION 15: Regulatory information This substance/mixture is not intended to be transported in bulk Safety, health and environmental regulations/legislation specific for the substance or mixture EU regulations Regulation (EC) No. 1005/2009 on substances that deplete the ozone layer, Annex I Regulation (EC) No. 1005/2009 on substances that deplete the ozone layer, Annex II Regulation (EC) No. 850/2004 On persistent organic pollutants, Annex I as amended Regulation (EC) No. 689/2008 concerning the export and import of dangerous chemicals, Annex I, part 1 as amended Regulation (EC) No. 689/2008 concerning the export and import of dangerous chemicals, Annex I, part 2 as amended Regulation (EC) No. 689/2008 concerning the export and import of dangerous chemicals, Annex I, part 3 as amended Regulation (EC) No. 689/2008 concerning the export and import of dangerous chemicals, Annex V as amended Regulation (EC) No. 166/2006 Annex II Pollutant Release and Transfer Registry Regulation (EC) No. 1907/2006, REACH Article 59(1) Candidate List as currently published by ECHA Authorisations Regulation (EC) No. 143/2011 Annex XIV Substances Subject to Authorisation Restrictions on use Regulation (EC) No. 1907/2006, REACH Annex XVII Substances subject to restriction on marketing and use as amended Directive 2004/37/EC: on the protection of workers from the risks related to exposure to carcinogens and mutagens at work Not regulated. Directive 92/85/EEC: on the safety and health of pregnant workers and workers who have recently given birth or are breastfeeding Not regulated. Other EU regulations Directive 96/82/EC (Seveso II) on the control of major-accident hazards involving dangerous substances Not regulated. Directive 98/24/EC on the protection of the health and safety of workers from the risks related to chemical agents at work 5 / 6

30 Directive 94/33/EC on the protection of young people at work Other regulations National regulations Chemical safety assessment SECTION 16: Other information The product is classified and labelled in accordance with Regulation (EC) 1272/2008 (CLP Regulation) as amended and respective national laws implementing EC directives. This Safety Data Sheet complies with the requirements of Regulation (EC) No 1907/2006. Follow national regulation for work with chemical agents. No Chemical Safety Assessment has been carried out. List of abbreviations References Information on evaluation method leading to the classification of mixture Full text of any statements or R-phrases and H-statements under Sections 2 to 15 Training information Disclaimer DNEL: Derived No-Effect Level. PNEC: Predicted No-Effect Concentration. PBT: Persistent, bioaccumulative and toxic. vpvb: Very Persistent and very Bioaccumulative. CLP: Regulation No. 1272/2008. DSD: Directive 67/548/EEC. HSDB - Hazardous Substances Data Bank Registry of Toxic Effects of Chemical Substances (RTECS) The mixture is classified based on test data for physical hazards. The classification for health and environmental hazards is derived by a combination of calculation methods and test data, if available. For details, refer to Sections 9, 11 and 12. R36/37/38 Irritating to eyes, respiratory system and skin. H315 - Causes skin irritation. H319 - Causes serious eye irritation. H335 - May cause respiratory irritation. Follow training instructions when handling this material. The information in the sheet was written based on the best knowledge and experience currently available. 6 / 6

31 SAFETY DATA SHEET SECTION 1: Identification of the substance/mixture and of the company/undertaking 1.1. Product identifier Trade name or designation N-Glycanase of the mixture Registration number - Synonyms PNGase F SDS number SDS WS0278 Product code WS0278 Issue date 08-April-2014 Version number AD Revision date - Supersedes date 24-May Relevant identified uses of the substance or mixture and uses advised against Identified uses Research and development. Uses advised against None known Details of the supplier of the safety data sheet Supplier Company name ProZyme, Inc. Address 3832 Bay Center Place Hayward, CA Division Telephone Contact person 1.4. Emergency telephone number SECTION 2: Hazards identification 2.1. Classification of the substance or mixture The mixture has been assessed and/or tested for its physical, health and environmental hazards and the following classification applies. Classification according to Directive 67/548/EEC or 1999/45/EC as amended Classification R42/43 The full text for all R-phrases is displayed in section 16. Classification according to Regulation (EC) No 1272/2008 as amended Health hazards Respiratory sensitisation Skin sensitisation Category 1 Category 1 Hazard summary Physical hazards Not classified for physical hazards. Health hazards May cause sensitisation by inhalation and skin contact. Environmental hazards Not classified for hazards to the environment. Specific hazards None known. Main symptoms May cause an allergic skin reaction. Dermatitis. Rash. Sensitisation Label elements Label according to Regulation (EC) No. 1272/2008 as amended Contains: N-Glycanase H334 - May cause allergy or asthma symptoms or breathing difficulties if inhaled. H317 - May cause an allergic skin reaction. N-Glycanase SDS EU Version No.: AD Revision date: - Issue date: 08-April / 7

32 Hazard pictograms Signal word Hazard statements Precautionary statements Prevention Response Storage Disposal Danger Supplemental label information 2.3. Other hazards None known. H317 - May cause an allergic skin reaction. H334 - May cause allergy or asthma symptoms or breathing difficulties if inhaled. P261 - Avoid breathing mist or vapour. P272 - Contaminated work clothing should not be allowed out of the workplace. P280 - Wear protective gloves. P285 - In case of inadequate ventilation wear respiratory protection. P302 + P352 - IF ON SKIN: Wash with plenty of soap and water. P304 + P341 - IF INHALED: If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing. P321 - Specific treatment (see this label). P333 + P313 - If skin irritation or rash occurs: Get medical advice/attention. P342 + P311 - If experiencing respiratory symptoms: Call a POISON CENTRE or doctor / physician. P363 - Wash contaminated clothing before reuse. Store away from incompatible materials. P501 - Dispose of contents/container in accordance with local/regional/national/international regulations. SECTION 3: Composition/information on ingredients 3.2. Mixtures General information Chemical name % CAS-No. / EC No. REACH Registration No. INDEX No. Notes N-Glycanase < Classification: DSD: CLP: R42/43 Skin Sens. 1;H317, Resp. Sens. 1;H CLP: Regulation No. 1272/2008. DSD: Directive 67/548/EEC. M: M-factor vpvb: very persistent and very bioaccumulative substance. PBT: persistent, bioaccumulative and toxic substance. #: This substance has been assigned Community workplace exposure limit(s). Composition comments The full text for all R- and H-phrases is displayed in section 16. SECTION 4: First aid measures General information Ensure that medical personnel are aware of the material(s) involved, and take precautions to protect themselves. Wash contaminated clothing before reuse Description of first aid measures Inhalation Move to fresh air. If breathing is difficult, give oxygen. If breathing stops, provide artificial respiration. Call a physician if symptoms develop or persist. Skin contact Remove contaminated clothing immediately and wash skin with soap and water. If skin irritation or rash occurs: Get medical advice/attention. Wash clothing separately before reuse. Eye contact Rinse with water. Get medical attention if irritation develops and persists. Ingestion Rinse mouth. Get medical attention if symptoms occur Most important symptoms May cause allergic respiratory reaction. May cause an allergic skin reaction. Dermatitis. Rash. and effects, both acute and delayed 4.3. Indication of any immediate medical attention and special treatment needed SECTION 5: Firefighting measures General fire hazards N-Glycanase Provide general supportive measures and treat symptomatically. Keep victim under observation. Symptoms may be delayed. No unusual fire or explosion hazards noted. SDS EU Version No.: AD Revision date: - Issue date: 08-April / 7

33 5.1. Extinguishing media Suitable extinguishing media Unsuitable extinguishing media 5.2. Special hazards arising from the substance or mixture 5.3. Advice for firefighters Special protective equipment for firefighters Special fire fighting procedures Water fog. Foam. Dry chemical powder. Carbon dioxide (CO2). Do not use water jet as an extinguisher, as this will spread the fire. During fire, gases hazardous to health may be formed. Self-contained breathing apparatus and full protective clothing must be worn in case of fire. In case of fire and/or explosion do not breathe fumes. Move containers from fire area if you can do so without risk. SECTION 6: Accidental release measures 6.1. Personal precautions, protective equipment and emergency procedures For non-emergency Keep unnecessary personnel away. Keep upwind. Wear appropriate protective equipment and personnel clothing during clean-up. Avoid inhalation of vapours or mists. Do not touch damaged containers or spilled material unless wearing appropriate protective clothing. Ensure adequate ventilation. Local authorities should be advised if significant spillages cannot be contained. For personal protection, see section 8. For emergency responders Keep unnecessary personnel away. Use personal protection recommended in section 8 of the SDS Environmental precautions Avoid discharge into drains, water courses or onto the ground Methods and material for containment and cleaning up Large Spills: Stop the flow of material, if this is without risk. Dike the spilled material, where this is possible. Absorb in vermiculite, dry sand or earth and place into containers. Following product recovery, flush area with water. Small Spills: Wipe up with absorbent material (e.g. cloth, fleece). Clean surface thoroughly to remove residual contamination Reference to other sections Never return spills to original containers for re-use. For personal protection, see section 8. For waste disposal, see section 13. SECTION 7: Handling and storage 7.1. Precautions for safe handling 7.2. Conditions for safe storage, including any incompatibilities 7.3. Specific end use(s) SECTION 8: Exposure controls/personal protection 8.1. Control parameters Occupational exposure limits Biological limit values Recommended monitoring procedures Derived no-effect level (DNEL) Wear appropriate personal protective equipment. Avoid breathing mist or vapour. Avoid contact with eyes, skin, and clothing. Provide adequate ventilation. Observe good industrial hygiene practices. Keep container tightly closed. Store at 2-8 C (35-46 F). Store away from incompatible materials (see section 10 of the SDS). No exposure limits noted for ingredient(s). No biological exposure limits noted for the ingredient(s). Follow standard monitoring procedures. Predicted no effect concentrations (PNECs) 8.2. Exposure controls Appropriate engineering controls Good general ventilation (typically 10 air changes per hour) should be used. Ventilation rates should be matched to conditions. If applicable, use process enclosures, local exhaust ventilation, or other engineering controls to maintain airborne levels below recommended exposure limits. If exposure limits have not been established, maintain airborne levels to an acceptable level. Individual protection measures, such as personal protective equipment General information Personal protection equipment should be chosen according to the CEN standards and in discussion with the supplier of the personal protective equipment. Eye/face protection Wear safety glasses with side shields (or goggles). Wear a full-face respirator, if needed. Skin protection N-Glycanase SDS EU Version No.: AD Revision date: - Issue date: 08-April / 7

34 - Hand protection Wear appropriate chemical resistant gloves. - Other Wear suitable protective clothing. Respiratory protection Thermal hazards Hygiene measures Environmental exposure controls SECTION 9: Physical and chemical properties If engineering controls do not maintain airborne concentrations below recommended exposure limits (where applicable) or to an acceptable level (in countries where exposure limits have not been established), an approved respirator must be worn. Wear appropriate thermal protective clothing, when necessary. Always observe good personal hygiene measures, such as washing after handling the material and before eating, drinking, and/or smoking. Routinely wash work clothing and protective equipment to remove contaminants. Contaminated work clothing should not be allowed out of the workplace. Environmental manager must be informed of all major releases Information on basic physical and chemical properties Appearance Physical state Liquid. Form Liquid. Colour Clear. Odour Odour threshold ph Melting point/freezing point Initial boiling point and boiling range Flash point Evaporation rate Flammability (solid, gas) Upper/lower flammability or explosive limits Flammability limit - lower (%) Flammability limit - upper (%) Vapour pressure Vapour density Relative density Solubility(ies) Partition coefficient (n-octanol/water) Auto-ignition temperature Decomposition temperature Viscosity Explosive properties Oxidizing properties 9.2. Other information No relevant additional information available. SECTION 10: Stability and reactivity Reactivity The product is stable and non-reactive under normal conditions of use, storage and transport Chemical stability Material is stable under normal conditions Possibility of hazardous No dangerous reaction known under conditions of normal use. reactions Conditions to avoid Contact with incompatible materials Incompatible materials Strong oxidising agents Hazardous No hazardous decomposition products are known. decomposition products SECTION 11: Toxicological information General information Occupational exposure to the substance or mixture may cause adverse effects. N-Glycanase SDS EU Version No.: AD Revision date: - Issue date: 08-April / 7

35 Information on likely routes of exposure Ingestion May cause discomfort if swallowed. Inhalation May cause sensitisation by inhalation. Skin contact May cause sensitisation by skin contact. Eye contact Direct contact with eyes may cause temporary irritation. Symptoms Dermatitis. Rash. Difficulty in breathing Information on toxicological effects Acute toxicity Skin corrosion/irritation Serious eye damage/eye irritation Respiratory sensitisation Skin sensitisation Germ cell mutagenicity Carcinogenicity Reproductive toxicity Specific target organ toxicity - single exposure Specific target organ toxicity - repeated exposure Aspiration hazard Mixture versus substance information Other information Due to lack of data the classification is not possible. Due to lack of data the classification is not possible. Due to lack of data the classification is not possible. May cause allergy or asthma symptoms or breathing difficulties if inhaled. May cause an allergic skin reaction. Due to lack of data the classification is not possible. Due to lack of data the classification is not possible. Due to lack of data the classification is not possible. Due to lack of data the classification is not possible. Due to lack of data the classification is not possible. Due to lack of data the classification is not possible. No information available. SECTION 12: Ecological information Toxicity The product is not classified as environmentally hazardous. However, this does not exclude the possibility that large or frequent spills can have a harmful or damaging effect on the environment Persistence and No data is available on the degradability of this product. degradability Bioaccumulative potential Partition coefficient n-octanol/water (log Kow) Bioconcentration factor (BCF) Mobility in soil Results of PBT and vpvb assessment Other adverse effects No other adverse environmental effects (e.g. ozone depletion, photochemical ozone creation potential, endocrine disruption, global warming potential) are expected from this component. SECTION 13: Disposal considerations Waste treatment methods Residual waste Contaminated packaging EU waste code Disposal methods/information Special precautions Dispose of in accordance with local regulations. Empty containers or liners may retain some product residues. This material and its container must be disposed of in a safe manner (see: Disposal instructions). Empty containers should be taken to an approved waste handling site for recycling or disposal. Since emptied containers may retain product residue, follow label warnings even after container is emptied. The Waste code should be assigned in discussion between the user, the producer and the waste disposal company. Collect and reclaim or dispose in sealed containers at licensed waste disposal site. Dispose of contents/container in accordance with local/regional/national/international regulations. Dispose in accordance with all applicable regulations. SECTION 14: Transport information ADR Not regulated as dangerous goods. N-Glycanase SDS EU Version No.: AD Revision date: - Issue date: 08-April / 7

36 RID Not regulated as dangerous goods. ADN Not regulated as dangerous goods. IATA Not regulated as dangerous goods. IMDG Not regulated as dangerous goods Transport in bulk according to Annex II of MARPOL 73/78 and the IBC Code SECTION 15: Regulatory information This substance/mixture is not intended to be transported in bulk Safety, health and environmental regulations/legislation specific for the substance or mixture EU regulations Regulation (EC) No. 1005/2009 on substances that deplete the ozone layer, Annex I Regulation (EC) No. 1005/2009 on substances that deplete the ozone layer, Annex II Regulation (EC) No. 850/2004 On persistent organic pollutants, Annex I as amended Regulation (EC) No. 689/2008 concerning the export and import of dangerous chemicals, Annex I, part 1 as amended Regulation (EC) No. 689/2008 concerning the export and import of dangerous chemicals, Annex I, part 2 as amended Regulation (EC) No. 689/2008 concerning the export and import of dangerous chemicals, Annex I, part 3 as amended Regulation (EC) No. 689/2008 concerning the export and import of dangerous chemicals, Annex V as amended Regulation (EC) No. 166/2006 Annex II Pollutant Release and Transfer Registry Regulation (EC) No. 1907/2006, REACH Article 59(1) Candidate List as currently published by ECHA Authorisations Regulation (EC) No. 1907/2006, REACH Annex XIV Substances subject to authorisation, as amended Restrictions on use Regulation (EC) No. 1907/2006, REACH Annex XVII Substances subject to restriction on marketing and use as amended Directive 2004/37/EC: on the protection of workers from the risks related to exposure to carcinogens and mutagens at work Directive 92/85/EEC: on the safety and health of pregnant workers and workers who have recently given birth or are breastfeeding Other EU regulations Directive 96/82/EC (Seveso II) on the control of major-accident hazards involving dangerous substances Directive 94/33/EC on the protection of young people at work Other regulations The product is classified and labelled in accordance with EC directives or respective national laws. This Safety Data Sheet complies with the requirements of Regulation (EC) No 1907/2006. National regulations Young people under 18 years old are not allowed to work with this product according to the EU Directive 94/33/EC on the protection of young people at work. Follow national regulation for work with chemical agents Chemical safety No Chemical Safety Assessment has been carried out. assessment N-Glycanase SDS EU Version No.: AD Revision date: - Issue date: 08-April / 7

37 SECTION 16: Other information List of abbreviations LD50: Lethal Dose, 50%. References Information on evaluation method leading to the classification of mixture Full text of any statements or R-phrases and H-statements under Sections 2 to 15 Training information Disclaimer The classification for health and environmental hazards is derived by a combination of calculation methods and test data, if available. R42/43 May cause sensitisation by inhalation and skin contact. H317 May cause an allergic skin reaction. H334 May cause allergy or asthma symptoms or breathing difficulties if inhaled. Follow training instructions when handling this material. The information in this (M)SDS was obtained from sources which we believe are reliable but cannot guarantee. Additionally, your use of this information is beyond our control and may be beyond our knowledge. Therefore, the information is provided without any representation or warranty express or implied. Trademarks ProZyme and N-Glycanase are trademarks or registered trademarks in the United States and other countries. N-Glycanase SDS EU Version No.: AD Revision date: - Issue date: 08-April / 7

38

39 SAFETY DATA SHEET SECTION 1: Identification of the substance/mixture and of the company/undertaking 1.1. Product identifier Trade name or designation of the mixture Registration number Synonyms SDS number Product code Issue date Version number Revision date Supersedes date 2-AB Solution - None. WS0302 WS January-2013 AD 05-August May Relevant identified uses of the substance or mixture and uses advised against Identified uses Uses advised against Research and development. None known Details of the supplier of the safety data sheet Supplier Company name Address Telephone Contact person 1.4. Emergency telephone number ProZyme, Inc Bay Center Place, Hayward CA United States SECTION 2: Hazards identification 2.1. Classification of the substance or mixture Classification according to Directive 67/548/EEC or 1999/45/EC as amended Classification C;R34, Xi;R37 Classification according to Regulation (EC) No 1272/2008 as amended Health hazards Skin corrosion/irritation Specific target organ toxicity - single exposure Category 1B Category 3 respiratory tract irritation H314 - Causes severe skin burns and eye damage. H335 - May cause respiratory irritation. Hazard summary Physical hazards Not classified for physical hazards. Health hazards Causes burns. Irritating to respiratory system. Environmental hazards Not classified for hazards to the environment. Specific hazards Ingestion may cause irritation and malaise. Main symptoms Exposed may experience eye tearing, redness, and discomfort Label elements Label according to Regulation (EC) No. 1272/2008 as amended Contains: 2-Aminoben zamide, Acetic acid, Dimethyl sulfoxide Hazard pictograms Signal word Danger Revision No.: AD Revision Date: 05 August / 12

40 Hazard statements Precautionary statements Prevention Response Storage Disposal Supplemental label information H314 - Causes severe skin burns and eye damage. H335 - May cause respiratory irritation. P280 - Wear protective gloves/protective clothing/eye protection/face protection. P264 - Wash thoroughly after handling. P260 - Do not breathe mist or vapour. P271 - Use only outdoors or in a well-ventilated area. P301 + P330 + P331 - IF SWALLOWED: rinse mouth. Do NOT induce vomiting. P303 + P361 + P353 - IF ON SKIN (or hair): Remove/Take off immediately all contaminated clothing. Rinse skin with water/shower. P363 - Wash contaminated clothing before re-use. P304 + P340 - IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing. P305 + P351 + P338 - IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Take off contaminated clothing and wash before re-use. P405 - Store locked up. P403 + P233 - Store in a well-ventilated place. Keep container tightly closed. P501 - Dispose of contents/container in accordance with local/regional/national/international regulations Other hazards Not a PBT or vpvb substance or mixture. SECTION 3: Composition/information on ingredients 3.2. Mixtures General information Chemical name % CAS-No. / EC No. REACH Registration No. INDEX No. Notes Dimethyl sulfoxide Classification: DSD: Xi;R36/37/38 CLP: Skin Irrit. 2;H315, Eye Irrit. 2;H319, STOT SE 3;H Acetic acid # Classification: DSD: R10, C;R35 CLP: Flam. Liq. 3;H226, Skin Corr. 1A;H314 2-Aminobenzamide Classification: DSD: Xi;R36 CLP: Skin Irrit. 2;H315 SECTION 4: First aid measures General information 4.1. Description of first aid measures Inhalation Skin contact Eye contact Ingestion 4.2. Most important symptoms and effects, both acute and delayed Ensure that medical personnel are aware of the material(s) involved, and take precautions to protect themselves. Move injured person into fresh air and keep person calm under observation. Get medical attention if symptoms occur. Immediately flush with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Wash contaminated clothing before reuse. Destroy or thoroughly clean contaminated shoes. Get medical attention immediately! Immediately flush with plenty of water for at least 15 minutes. If easy to do, remove contact lenses. Get medical attention immediately. Call a physician or poison control center immediately. Never give anything by mouth to an unconscious person. Do not induce vomiting. Exposed may experience eye tearing, redness, and discomfort. 2 / 12

41 4.3. Indication of any immediate medical attention and special treatment needed Treat symptomatically. SECTION 5: Firefighting measures General fire hazards 5.1. Extinguishing media Suitable extinguishing media Unsuitable extinguishing media 5.2. Special hazards arising from the substance or mixture 5.3. Advice for firefighters Special protective equipment for firefighters Special fire fighting procedures The product is combustible. Water. Water fog. Foam. Dry chemical powder. Carbon dioxide (CO2). None known. SECTION 6: Accidental release measures During fire, gases hazardous to health may be formed. Self-contained breathing apparatus and full protective clothing must be worn in case of fire. Use standard firefighting procedures and consider the hazards of other involved materials Personal precautions, protective equipment and emergency procedures For non-emergency personnel Avoid contact with skin and eyes. Wear appropriate personal protective equipment. For emergency responders Keep unnecessary personnel away. Use personal protection as recommended in section 8 of the SDS Environmental precautions Avoid discharge into drains, water courses or onto the ground Methods and material for containment and cleaning up 6.4. Reference to other sections SECTION 7: Handling and storage 7.1. Precautions for safe handling 7.2. Conditions for safe storage, including any incompatibilities 7.3. Specific end use(s) Reagent. SECTION 8: Exposure controls/personal protection 8.1. Control parameters Occupational exposure limits Austria. MAK List Wipe up with absorbent material (e.g. cloth, fleece). Flush area with plenty of water. For personal protection, see section 8. For waste disposal, see section 13. Avoid contact with eyes, skin, and clothing. Do not taste or swallow. Wear appropriate personal protective equipment. Wash thoroughly after handling. Change contaminated clothing. Keep away from heat, sparks and open flame. Keep containers tightly closed in a cool, well-ventilated place. Store away from incompatible materials. Store at -20 C (-4 F). Dimethyl sulfoxide (CAS ) Belgium. Exposure Limit s. Ceiling MAK MAK 50 mg/m3 20 ppm 25 mg/m3 10 ppm 160 mg/m3 50 ppm STEL 38 mg/m3 15 ppm 25 mg/m3 10 ppm Bulgaria. OELs. Regulation No 13 on protection of workers against risks of exposure to chemical agents at work STEL 37 mg/m3 3 / 12

42 Bulgaria. OELs. Regulation No 13 on protection of workers against risks of exposure to chemical agents at work 25 mg/m3 Cyprus. OELs. Control of factory atmosphere and dangerous substances in factories regulation, PI 311/73, as amended. Czech Republic. OELs. Government Decree mg/m3 10 ppm Denmark. Exposure Limit s Ceiling 35 mg/m3 25 mg/m3 Dimethyl sulfoxide (CAS ) TLV TLV 25 mg/m3 10 ppm 160 mg/m3 50 ppm Estonia. OELs. Occupational Exposure Limits of Hazardous Substances. (Annex of Regulation No. 293 of 18 September 2001) Dimethyl sulfoxide (CAS ) Finland. Workplace Exposure Limits STEL STEL 25 mg/m3 10 ppm 25 mg/m3 10 ppm 500 mg/m3 150 ppm 150 mg/m3 50 ppm Dimethyl sulfoxide (CAS ) STEL 25 mg/m3 10 ppm 13 mg/m3 5 ppm 50 ppm France. Threshold Limit s (VLEP) for Occupational Exposure to Chemicals in France, INRS ED 984 VLE 25 mg/m3 10 ppm Germany. DFG MAK List (advisory OELs). Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area (DFG) Dimethyl sulfoxide (CAS ) Germany. TRGS 900, Limit s in the Ambient Air at the Workplace 25 mg/m3 10 ppm 160 mg/m3 50 ppm AGW 25 mg/m3 10 ppm 4 / 12

43 Greece. OELs (Decree No. 90/1999, as amended) STEL Hungary. OELs. Joint Decree on Chemical Safety of Workplaces STEL Iceland. OELs. Regulation 154/1999 on occupational exposure limits 37 mg/m3 15 ppm 25 mg/m3 10 ppm 25 mg/m3 25 mg/m3 Ireland. Occupational Exposure Limits Italy. OELs STEL 25 mg/m3 10 ppm 37 mg/m3 15 ppm 25 mg/m3 10 ppm 25 mg/m3 10 ppm Latvia. OELs. Occupational exposure limit values of chemical substances in work environment 25 mg/m3 10 ppm Lithuania. OELs. Limit s for Chemical Substances, General Requirements (Hygiene Norm HN 23:2007) Dimethyl sulfoxide (CAS ) STEL Luxembourg. Binding Occupational exposure limit values (Annex I), Memorial A 25 mg/m3 10 ppm 500 mg/m3 150 ppm 150 mg/m3 50 ppm 25 mg/m3 10 ppm Malta. OELs. Occupational Exposure Limit s (L.N of Occupational Health and Safety Authority Act (CAP. 424), Schedules I and V) Norway. Administrative Norms for Contaminants in the Workplace 25 mg/m3 10 ppm TLV 25 mg/m3 10 ppm 5 / 12

44 Poland. MACs. Minister of Labour and Social Policy Regarding Maximum Allowable Concentrations and Intensities in Working Environment STEL 30 mg/m3 15 mg/m3 Portugal. OELs. Decree-Law n. 290/2001 (Journal of the Republic - 1 Series A, n.266) 25 mg/m3 10 ppm Portugal. VLEs. Norm on occupational exposure to chemical agents (NP 1796) STEL 15 ppm 10 ppm Romania. OELs. Protection of workers from exposure to chemical agents at the workplace 25 mg/m3 10 ppm Slovakia. OELs. Decree of the government of the Slovak Republic concerning protection of health in work with chemical agents 25 mg/m3 10 ppm Slovenia. OELs. Regulations concerning protection of workers against risks due to exposure to chemicals while working (Official Gazette of the Republic of Slovenia) Dimethyl sulfoxide (CAS ) Spain. Occupational Exposure Limits 25 mg/m3 10 ppm 160 mg/m3 STEL Sweden. Occupational Exposure Limit s Dimethyl sulfoxide (CAS ) STEL STEL Switzerland. SUVA Grenzwerte am Arbeitsplatz 37 mg/m3 15 ppm 25 mg/m3 10 ppm 25 mg/m3 10 ppm 13 mg/m3 5 ppm 500 mg/m3 150 ppm 150 mg/m3 50 ppm Dimethyl sulfoxide (CAS ) STEL STEL 50 mg/m3 20 ppm 25 mg/m3 10 ppm 320 mg/m3 100 ppm 160 mg/m3 6 / 12

45 Switzerland. SUVA Grenzwerte am Arbeitsplatz 50 ppm EU. Indicative Exposure Limit s in Directives 91/322/EEC, 2000/39/EC, 2006/15/EC, 2009/161/EU Biological limit values Recommended monitoring procedures No biological exposure limits noted for the ingredient(s). Follow standard monitoring procedures. 25 mg/m3 10 ppm Derived no-effect level (DNEL) Route Workers Inhalation 25 mg/m3 Acute Local effects Inhalation 25 mg/m3 Long term Local effects Predicted no effect concentrations (PNECs) Route Aqua (freshwater) Water 3,058 mg/l Aqua (intermittent releases) Water 30,58 mg/l Aqua (marine water) Water 0,3058 mg/l Sediment Not applicable 11,36 mg/kg (freshwater) Sediment (marine Not applicable 1,136 mg/kg water) Sewage Treatment Not applicable 85 mg/l Plant Soil Soil 0,47 mg/kg 8.2. Exposure controls Appropriate engineering controls Form Form No special requirements under ordinary conditions of use and with adequate ventilation. Individual protection measures, such as personal protective equipment General information Eye/face protection Skin protection Personal protective equipment should be chosen according to the CEN standards and in discussion with the supplier of the personal protective equipment. Wear safety glasses with side shields (or goggles). - Hand protection Use protective gloves. Suitable gloves can be recommended by the glove supplier. - Other Wear suitable protective clothing. Respiratory protection Thermal hazards Hygiene measures Environmental exposure controls Under normal conditions, respirator is not normally required. SECTION 9: Physical and chemical properties 9.1. Information on basic physical and chemical properties Appearance Odour Physical state Form Colour Odour threshold ph 8,8 Melting point/freezing point Initial boiling point and boiling range Flash point Evaporation rate Handle in accordance with good industrial hygiene and safety practices. Environmental manager must be informed of all major releases. Clear to straw colored liquid. Liquid. Liquid. Acetic acid. 7 / 12

46 Flammability (solid, gas) Upper/lower flammability or explosive limits Flammability limit - lower (%) Flammability limit - upper (%) Vapour pressure Vapour density Relative density Solubility(ies) Partition coefficient (n-octanol/water) Decomposition temperature Viscosity Explosive properties Oxidizing properties 9.2. Other information No relevant additional information available. SECTION 10: Stability and reactivity Reactivity The product is non-reactive under normal conditions of use, storage and transport Chemical stability Material is stable under normal conditions Possibility of hazardous reactions Hazardous polymerisation does not occur Conditions to avoid Keep away from heat, sparks and open flame Incompatible materials Strong oxidizing agents, acid chlorides, alkali metals, hydrobromic acid, acidic solutions of alkali bromides Hazardous decomposition products SECTION 11: Toxicological information General information Information on likely routes of exposure Ingestion Inhalation Skin contact Eye contact Symptoms Information on toxicological effects Carbon oxides. Nitrogen oxides. Occupational exposure to the substance or mixture may cause adverse effects. Ingestion may produce burns to the lips, oral cavity, upper airway, esophagus and possibly the digestive tract. May cause respiratory tract irritation. Causes skin burns. Causes serious eye damage. Exposed may experience eye tearing, redness, and discomfort. Acute toxicity Acute Dermal LD50 Inhalation LC50 Oral LD50 Dimethyl sulfoxide (CAS ) Acute Oral LD50 Other LD50 Skin corrosion/irritation Ingestion may cause irritation and malaise. Species Rabbit Rat Rat Rat Rat Causes skin burns. Test results 1060 mg/kg 11,4 mg/l, 4 Hours 3,31 g/kg 17,9 ml/kg mg/kg 8 / 12

47 Serious eye damage/irritation Respiratory sensitisation Skin sensitisation Germ cell mutagenicity Carcinogenicity Reproductive toxicity Specific target organ toxicity - single exposure Specific target organ toxicity - repeated exposure Aspiration hazard Mixture versus substance information Other information Causes serious eye damage. Not a skin sensitiser. May cause respiratory irritation. SECTION 12: Ecological information Toxicity Not expected to be harmful to aquatic organisms. 2-Aminobenzamide (CAS ) Aquatic Fish Aquatic Crustacea Fish Dimethyl sulfoxide (CAS ) Aquatic Fish LC50 EC50 LC50 LC50 Species Fathead minnow (Pimephales promelas) Water flea (Daphnia magna) Bluegill (Lepomis macrochirus) Fathead minnow (Pimephales promelas) Rainbow trout,donaldson trout (Oncorhynchus mykiss) Test results mg/l, 96 hours 65 mg/l, 48 hours 75 mg/l, 96 hours 79 mg/l, 96 Hours mg/l, 96 hours Persistence and degradability Bioaccumulative potential The product is not expected to be readily biodegradable. Partition coefficient n-octanol/water (log Kow) -0,17 Dimethyl sulfoxide (CAS ) -2,03 Bioconcentration factor (BCF) Mobility in soil Results of PBT and vpvb assessment Other adverse effects None known. SECTION 13: Disposal considerations Waste treatment methods Residual waste Contaminated packaging EU waste code * Disposal methods/information SECTION 14: Transport information Not a PBT or vpvb substance or mixture. Dispose of in accordance with local regulations. Since emptied containers retain product residue, follow label warnings even after container is emptied. Dispose in accordance with all applicable regulations. ADR UN number UN proper shipping name UN2790 ACETIC ACID SOLUTION 9 / 12

48 14.3. Transport hazard class(es) Class 8 Subsidiary risk - Label(s) 8 Hazard No. (ADR) 80 Tunnel restriction code E Packing group II Environmental hazards No Special precautions for user RID UN number UN UN proper shipping ACETIC ACID SOLUTION name Transport hazard class(es) Class 8 Subsidiary risk - Label(s) Packing group II Environmental hazards No Special precautions for user ADN UN number UN UN proper shipping acetic acid name Transport hazard class(es) Class 8 Subsidiary risk - Label(s) Packing group II Environmental hazards No Special precautions for user IATA UN number UN UN proper shipping Acetic acid solution name Transport hazard class(es) Class 8 Subsidiary risk Packing group II Environmental hazards No. ERG Code 8L Special precautions for user IMDG UN number UN UN proper shipping ACETIC ACID SOLUTION name Transport hazard class(es) Class 8 Subsidiary risk Packing group Environmental hazards Marine pollutant EmS Special precautions for user Transport in bulk according to Annex II of MARPOL 73/78 and the IBC Code - II No. F-A, S-B This substance/mixture is not intended to be transported in bulk. 10 / 12

49 SECTION 15: Regulatory information Safety, health and environmental regulations/legislation specific for the substance or mixture EU regulations Regulation (EC) No. 1005/2009 on substances that deplete the ozone layer, Annex I Regulation (EC) No. 1005/2009 on substances that deplete the ozone layer, Annex II Regulation (EC) No. 850/2004 On persistent organic pollutants, Annex I as amended Regulation (EC) No. 689/2008 concerning the export and import of dangerous chemicals, Annex I, part 1 as amended Regulation (EC) No. 689/2008 concerning the export and import of dangerous chemicals, Annex I, part 2 as amended Regulation (EC) No. 689/2008 concerning the export and import of dangerous chemicals, Annex I, part 3 as amended Regulation (EC) No. 689/2008 concerning the export and import of dangerous chemicals, Annex V as amended Regulation (EC) No. 166/2006 Annex II Pollutant Release and Transfer Registry Regulation (EC) No. 1907/2006, REACH Article 59(1) Candidate List as currently published by ECHA Authorisations Regulation (EC) No. 143/2011 Annex XIV Substances Subject to Authorisation Restrictions on use Regulation (EC) No. 1907/2006, REACH Annex XVII Substances subject to restriction on marketing and use as amended Directive 2004/37/EC: on the protection of workers from the risks related to exposure to carcinogens and mutagens at work Not regulated. Directive 92/85/EEC: on the safety and health of pregnant workers and workers who have recently given birth or are breastfeeding Not regulated. Other EU regulations Directive 96/82/EC (Seveso II) on the control of major-accident hazards involving dangerous substances Not regulated. Directive 98/24/EC on the protection of the health and safety of workers from the risks related to chemical agents at work Directive 94/33/EC on the protection of young people at work Other regulations National regulations Chemical safety assessment SECTION 16: Other information The product is classified and labelled in accordance with Regulation (EC) 1272/2008 (CLP Regulation) as amended and respective national laws implementing EC directives. This Safety Data Sheet complies with the requirements of Regulation (EC) No 1907/2006. Young people under 18 years old are not allowed to work with this product according to EU Directive 94/33/EC on the protection of young people at work. Follow national regulation for work with chemical agents. No Chemical Safety Assessment has been carried out. List of abbreviations References DNEL: Derived No-Effect Level. PNEC: Predicted No-Effect Concentration. PBT: Persistent, bioaccumulative and toxic. vpvb: Very Persistent and very Bioaccumulative. CLP: Regulation No. 1272/2008. DSD: Directive 67/548/EEC. HSDB - Hazardous Substances Data Bank Registry of Toxic Effects of Chemical Substances (RTECS) 11 / 12

50 Information on evaluation method leading to the classification of mixture Full text of any statements or R-phrases and H-statements under Sections 2 to 15 Training information Disclaimer Trademarks The mixture is classified based on test data for physical hazards. The classification for health and environmental hazards is derived by a combination of calculation methods and test data, if available. For details, refer to Sections 9, 11 and 12. R10 Flammable. R34 Causes burns. R35 Causes severe burns. R36 Irritating to eyes. R36/37/38 Irritating to eyes, respiratory system and skin. R37 Irritating to respiratory system. H226 - Flammable liquid and vapour. H314 - Causes severe skin burns and eye damage. H315 - Causes skin irritation. H319 - Causes serious eye irritation. H335 - May cause respiratory irritation. Follow training instructions when handling this material. The information in the sheet was written based on the best knowledge and experience currently available. ProZyme is a trademark or registered trademark of ProZyme Inc., in the United States and other countries. 12 / 12

51 SAFETY DATA SHEET SECTION 1: Identification of the substance/mixture and of the company/undertaking 1.1. Product identifier Trade name or designation of the mixture Registration number Synonyms SDS number Product code Issue date Version number Revision date Supersedes date Reductant Solution - None. SDS WS0300/WS WS0300 and WS January-2013 AE 03-July May Relevant identified uses of the substance or mixture and uses advised against Identified uses Uses advised against Research and development. None known Details of the supplier of the safety data sheet Supplier Company name Address Telephone Contact person 1.4. Emergency telephone number ProZyme, Inc Bay Center Place, Hayward CA United States SECTION 2: Hazards identification 2.1. Classification of the substance or mixture Classification according to Directive 67/548/EEC or 1999/45/EC as amended Classification T+;R26/27/28, C;R34, Xi;R37, R32, N;R51/53 Classification according to Regulation (EC) No 1272/2008 as amended Health hazards Acute toxicity, oral Acute toxicity, dermal Acute toxicity, inhalation Skin corrosion/irritation Specific target organ toxicity - single exposure Environmental hazards Hazardous to the aquatic environment, long-term aquatic hazard Category 2 Category 2 Category 2 Category 1B Category 3 respiratory tract irritation Category 2 H300 - Fatal if swallowed. H310 - Fatal in contact with skin. H314 - Causes severe skin burns and eye damage. H335 - May cause respiratory irritation. H411 - Toxic to aquatic life with long lasting effects. Hazard summary Physical hazards Health hazards Environmental hazards Specific hazards Main symptoms 2.2. Label elements Not classified for physical hazards. Very toxic by inhalation, in contact with skin and if swallowed. Contact with acids liberates very toxic gas. Causes burns. Irritating to respiratory system. Toxic to aquatic organisms, may cause long-term adverse effects in the aquatic environment. Ingestion may cause irritation and malaise. Exposed may experience eye tearing, redness, and discomfort. Cough. Shortness of breath. Swallowing can result in nausea, vomiting, diarrhoea, abdominal pain, convulsions and loss of consciousness. Collapse and possible death may occur. Revision No.: AE Revision Date: 03 Jun / 10

52 Label according to Regulation (EC) No. 1272/2008 as amended Contains: Sodium cyanotrihy drobo rate Hazard pictograms Signal word Hazard statements Precautionary statements Prevention Response Storage Disposal Danger Supplemental label information H300 - Fatal if swallowed. H310 - Fatal in contact with skin. H330 - Fatal if inhaled. H314 - Causes severe skin burns and eye damage. H335 - May cause respiratory irritation. H411 - Toxic to aquatic life with long lasting effects. P264 - Wash thoroughly after handling. P270 - Do not eat, drink or smoke when using this product. P262 - Do not get in eyes, on skin, or on clothing. P280 - Wear protective gloves/protective clothing/eye protection/face protection. P284 - Wear respiratory protection. P260 - Do not breathe mist or vapour. P271 - Use only outdoors or in a well-ventilated area. P222 - Do not allow contact with air. P231 + P232 - Handle under inert gas. Protect from moisture. P273 - Avoid release to the environment. P301 + P310 - IF SWALLOWED: Immediately call a POISON CENTRE or doctor/physician. P330 - Rinse mouth. P331 - Do NOT induce vomiting. P305 + P351 + P338 - IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P302 + P350 - IF ON SKIN: Gently wash with plenty of soap and water. P310 - Immediately call a POISON CENTRE or doctor/physician. P361 - Remove/Take off immediately all contaminated clothing. P363 - Wash contaminated clothing before re-use. P304 + P340 - IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing. P391 - Collect spillage. P405 - Store locked up. P403 + P233 - Store in a well-ventilated place. Keep container tightly closed. P402 + P404 - Store in a dry place. Store in a closed container. P501 - Dispose of contents/container in accordance with local/regional/national/international regulations Other hazards Not a PBT or vpvb substance or mixture. SECTION 3: Composition/information on ingredients 3.2. Mixtures General information Chemical name % CAS-No. / EC No. REACH Registration No. INDEX No. Notes Dimethyl sulfoxide Classification: DSD: Xi;R36/37/38 CLP: Skin Irrit. 2;H315, Eye Irrit. 2;H319, STOT SE 3;H Sodium cyanotrihydroborate Classification: DSD: F;R15, T+;R26/27/28, C;R34, R32, N;R50-53 CLP: Flam. Sol. 1;H228, Acute Tox. 2;H300, Acute Tox. 2;H310, Skin Corr. 1B;H314, Acute Tox. 2;H330, Aquatic Acute 1;H400, Aquatic Chronic 1;H / 10