INNOVATING FOR A GREENER WORLD WITH MINERAL-BASED ANTI MICROBIALS Presentation for To Watertech 2010 April 23, 2010.

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1 Richmond, British Columbia. INNOVATING FOR A GREENER WORLD WITH MINERAL-BASED ANTI MICROBIALS Presentation for To Watertech 2010 April 23, Frank R. Varseveld President / CEO EnvirEau Technologies Inc.

2 Historical uses of Minerals to Protect Water Quality Copper, Silver & Zinc have been used for centuries. Potable Water Urns Dressings for Wounds Internal Health Nutritional Supplements Veterinary Uses Enhanced Immunity

3 More Recently Copper Miners not Infected with Cholera Algae Control with Granular Copper Sulphate Silver in Water Filters Copper Sulphate with EDTA CHELATES Other Copper Compounds

4 Minerals in water aquo ions of the mineral These ions are active until affected by: High ph High Alkalinity Hardness Organics May Cause Instability Precipitation or Binding ION Speciation is very important. Most IONS are SHORT LIVED Most IONS are not BIOLOGICALLY Active

5 EXAMPLE: Cu++ is the most biologically active species of Copper ion. Total Copper at ph 5 At higher ph Hi Cu++ Cu++ reduces Cu (CO3) ) and CuOH+ + increases ions are more stable But decreased pesticidal effectiveness

6 To overcome this challenge, many combinations have been tried. Higher copper concentrations Organic chelations (citric Acid, EDTA) Ammonia Chlorine A few products using these formulations solutions are in use. Unmodified Aquo ION of Copper Cu [H2O]6)++

7 Our research led us to an inorganic ionizing agent which we now manufacture and add to all our formulations. It provides a combination of molecular stability and high pesticidal efficacy. This formulation offers the advantage of: Molecular stability in hostile environments - long shelf life - long-term protection High efficacy in a wide range of water quality Minimal environmental impact due to lower application rates

8 Products formulated with this Technology are described as being: A stable molecular matrix Energized aquo ions Capable of seek-and and-kill missions Selective microbial killers some examples:

9 ALGAE / BACTERIA CONTROL Dugouts Reservoirs Waterscapes Ponds Canals

10 Algae and Bacteria Control BEFORE TREATMENT 2 WEEKS AFTER TREATMENT

11 ALGAE/BIOFILM CONTROL MINERAL DE-SCALING Drip / Tape / Emitter Irrigation

12 ALGAE CONTROL / MINERAL DE-SCALING Eco-friendly treatment for elegant waterscapes

13 Algae / Bacteria Control Foliar Spray applications

14 ALGAE / BACTERIA CONTROL MINERAL DE-SCALING

15 ALGAE/BACTERIA CONTROL MINERAL DE-SCALING COOLING TOWERS HVAC SYSTEMS

16 ANAEROBIC BACTERIA / ODOUR CONTROL MANURE / SEWAGE FOG TREATMENT

17 Typical Sewage Lagoon Treatment Parameter BOD (biochemical oxygen demand) Faecal / Coli Bacteria TSS (total suspended solids) Starting Condition Resulting Condition Percentage Improvement , , Ammonia (ppm) Other Benefits: Few odors. No worms. No algae. Clear effluent

18 ANTI-BACTERIAL WASH WATER

19 Test Results Establishment Public des Laboratoires Departmentaux PARAMETERS Units Control Culturable Micro organisms 20 C ISO 6222 Culturable aerobic flora 36 C ISO 6222 E.Coli and coliform bacteria NF EN ISO Thermotolerant coliform organisms 44 C NFT Entestinal Enteroccoci NF EN ISO Sulphite-reducing bacteria NF EN 1SO CHLORI NE0.2 ml/l POLYDEX 2X1:120,000 MAX ALLOWABLE UFC/1 ml UFC/1 ml /100 ml /100 ml /100 ml /20 ML

20 Environmental Fate of mineralbased anti-microbials Concentrations are reduced to low ppb range Bonds with organics in a non-toxic form Consumed by plants, livestock or people Appears as a back-ground level of natural mineral in water or soil Becomes part of humic acid in marine sediments

21 Mineral-based Anti-Microbials offer opportunities to : Improve water quality / food safety Conserve water by recycling Reduce usage of synthetic chemicals Reduce costs Improve safety Reduce losses

22 Thank you for the opportunity to present Q & A?

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