2011 33 1 0157-0161 http / /xuebao. jxau. edu. cn Acta Agriculturae Universitatis Jiangxiensis E - mail ndxb7775@ sina. com * / 330045 2 300 mg /kg 200 mg /kg 100 mg /kg 3 mg /kg + 3 mg /kg + 200 mg / kg 4 2 TC TG HDL - C LDL - C TC LDL P < 0. 01 300 mg /kg /d Q946. 3 A 1000-2286 2011 01-0157 - 05 Studies on Hypolipemic Effect of Cyclocarya paliurus Batal Iljinskaja Polysaccharide HUANG Ming-quan SHANGGUAN Xin-chen XU Ming-sheng WANG Wen-jun * SHEN Yong-gen JIANG Yan CHEN Qin CHEN Ting-ting College of Food Science and Engineering /Research Lab for Plant Resource Development and Utilization Jiangxi Agricultural University Nanchang 330045 China Abstract The hypolipemic effect of Cyclocarya paliurus Batal Iljinskaja polysaccharide was study by in vivo and in vtro experments. The hypolipemic effect of Cyclocarya paliurus Batal Iljinskaja polysaccharide was detected in vitro by measuring the activity of pancreatic lipase. A test model of mice was made according to lipid level of the animals. The animals were divided into 7 groups the group with high dosages H polysaccharide 300 mg /kg the group with medium dosages M polysaccharide 200 mg /kg the group with low dosages L polysaccharide 100 mg /kg the group of simvastatin ST 3 mg /kg the group of simvastatin + medium dosages ST + M 3 mg /kg + 200 mg /kg the model group with hyperlipidemia and the normal group. All the mice were fed for. Then the influences of Cyclocarya paliurus Batal Iljinskaja polysaccharide on triglycerides TG total cholesterol TC low density lipoprotein cholesterol LDL and high density lipoprotein cholesterol HDL of serum in mice were determined every second week. Cyclocarya paliurus Batal Iljinskaja polysaccharide could inhibit the activity of pancreatic lipase in vitro. In the animal test polysaccharide could remarkably reduce the level of serum TC and 2010-09 - 09 2010-10 - 27 2002EA730001 2009BGA01900 2009GZN0014 YC09A067 1985 E - mail quanforice0620@ 163. com * E - mail wwjun9999@ sina. com
158 33 LDL P < 0. 01 the effect of high dosages was the best almost as effective as that of the group of sinvastatin. All this suggests that Cyclocarya paliurus Batal Iljinskaja polysaccharide has good hypolipemic effect. Key words Cyclocarya paliurus Batal Iljinskaja polysaccharide hyperlipemia hypolipemic effect 1 2 3-4 5-8 9 10-19 20 1 1. 1 1. 1. 1 10 5 h 2 2 sevage 70% 21-22 10% 89% 1% - 80 4 021-9601 1. 1. 2 TC TG HDL - C LDL - C Sigma 1. 2 1. 2. 1 23 100 ml 12 4 3 5 ml 0. 025 mol /L 4 ml 40 5 min 1 3 1 ml 30 min 95% 15 ml 3 0. 05 mol /L 3 2 3 1 ml 1 1 3 1. 2. 2 2 6 + 1. 2. 3 300 mg /kg 200 mg /kg 100 mg /kg 3 mg /kg 4 12 h TC TG HDL - C LDL - C 1. 2. 4 ± DPS V3. 01 2 2. 1 1 NaOH 3. 35 ml 2. 33 ml
1 159 NaOH Tab. 1 1 The activity of pancreatic lipase Lipase + Without lipase Polysaccharide + lipase Polysaccharide without lipase NaOH /ml 4. 30 3. 95 3. 80 0. 70 0. 65 0. 65 3. 50 3. 00 3. 30 0. 90 1. 00 0. 90 NaOH 4. 02 0. 67 3. 27 0. 93 Average NaOH 3. 35 2. 33 2. 2 2. 2. 1 TC 2 4 2 2 2 + P < 0. 01 4 4 + Tab. 2 2 TC The effect of polysaccharide on TC 2 Treatment for /% 4 Treatment for /% Normal 9 3. 93 ± 0. 88 3. 72 ± 1. 27 ** 5. 34 3. 88 ± 0. 71 ** 1. 27 H 15 10. 61 ± 3. 88 ** 4. 37 ± 1. 44 ** 58. 81 4. 21 ± 0. 35 ** 60. 32 M 14 8. 98 ± 1. 71 4. 34 ± 0. 58 ** 51. 67 4. 32 ± 0. 53 ** 51. 89 L 13 8. 23 ± 1. 63 4. 87 ± 1. 55 ** 40. 83 4. 15 ± 0. 58 ** 49. 57 ST + M 12 7. 82 ± 1. 61 3. 75 ± 1. 21 ** 52. 05 3. 05 ± 0. 44 ** 61. 00 ST 14 8. 91 ± 2. 41 3. 97 ± 0. 91 ** 55. 44 3. 68 ± 0. 78 ** 58. 70 Model 12 9. 46 ± 1. 79 * 6. 29 ± 1. 37 33. 51 6. 05 ± 0. 82 36. 05 * P < 0. 05 ** P < 0. 01 2. 2. 2 TG 2 4 3 3 TG Tab. 3 3 TG The effect of polysaccharide on TG 2 Treatment for /% 4 Treatment for /% Normal 9 1. 97 ± 0. 50 1. 83 ± 0. 93 7. 10 1. 88 ± 0. 21 4. 57 H 15 1. 97 ± 0. 49 1. 58 ± 0. 65 19. 80 1. 26 ± 0. 34 36. 04 M 14 1. 57 ± 0. 39 1. 39 ± 0. 70 11. 46 1. 15 ± 0. 59 26. 75 L 13 2. 38 ± 0. 37 1. 59 ± 0. 34 33. 19 1. 57 ± 0. 37 34. 03 ST + M 12 2. 07 ± 0. 59 1. 63 ± 0. 91 21. 26 1. 53 ± 0. 61 26. 09 ST 14 1. 46 ± 0. 59 1. 24 ± 0. 91 15. 07 1. 03 ± 0. 17 29. 45 Model 12 1. 56 ± 0. 58 1. 40 ± 0. 33 10. 26 1. 49 ± 0. 25 4. 49
160 33 2. 2. 3 HDL 2 4 4 4 2 4 HDL HDL HDL Tab. 4 4 HDL The effect of polysaccharide on HDL 2 Treatment for /% 4 Treatment for /% Normal 9 1. 49 ± 0. 68 1. 30 ± 0. 41 1. 45 ± 0. 37 H 15 1. 30 ± 0. 49 1. 44 ± 0. 42 10. 77 2. 03 ± 0. 42 ** 56. 15 M 14 1. 64 ± 0. 75 1. 42 ± 0. 56 2. 11 ± 0. 23 ** 28. 66 L 13 1. 44 ± 0. 56 1. 22 ± 0. 58 1. 89 ± 0. 46 ** 31. 25 ST + M 12 1. 13 ± 0. 39 1. 11 ± 0. 32 1. 99 ± 0. 58 ** 76. 11 ST 14 0. 93 ± 0. 67 0. 96 ± 0. 45 1. 45 ± 0. 46 * 55. 91 Model 12 1. 57 ± 0. 75 0. 81 ± 0. 31 3. 22 0. 83 ± 0. 11 * P < 0. 05 ** P < 0. 01 2. 2. 4 LDL 2 4 5 5 2 LDL P < 0. 01 4 2 + 2 LDL 5 LDL Tab. 5 The effect of polysaccharide on LDL 2 Treatment for /% 4 Treatment for /% Normal 9 0. 58 ± 0. 24 0. 71 ± 0. 24 ** 0. 68 ± 0. 11 ** H 15 4. 84 ± 0. 50 0. 63 ± 0. 22 ** 86. 98 0. 68 ± 0. 23 ** 85. 95 M 14 2. 92 ± 0. 84 0. 60 ± 0. 31 ** 79. 45 0. 65 ± 0. 12 ** 77. 74 L 13 3. 23 ± 1. 12 0. 79 ± 0. 56 ** 75. 54 0. 73 ± 0. 09 ** 77. 40 ST + M 12 3. 24 ± 1. 84 1. 07 ± 0. 76 ** 66. 98 0. 64 ± 0. 15 ** 80. 25 ST 14 3. 05 ± 1. 76 0. 72 ± 0. 48 ** 76. 39 0. 71 ± 0. 22 ** 76. 72 Model 12 4. 46 ± 1. 64 2. 72 ± 0. 50 39. 01 2. 93 ± 0. 51 34. 30 * P < 0. 05 ** P < 0. 01 3 NaOH 24 TC LDL
1 161 TG HDL 2 TC TG LDL HDL 25-3 27 TC LDL TG HDL + TC LDL P < 0. 01 TG HDL 300 mg / kg d 1. J. 2003 32 2 120-122. 2. J. 2003 25 2 212-214. 3. J. 2003 42 1 73-77. 4. J. 2006 28 6 809-812. 5. J. 2001 13 3 52-54. 6. J. 2003 31 6 117-120. 7. J. 2001 20 5 1-4. 8 Kurihara H Harukazu L Shibata H et al. Hypoglycemic action of Cyclocarya paliurus in normal and diabetic mice J. Biosci Biotechnol Biochem 2003 67 4 877-880. 9. J. 2004 16 5 59-60. 10. J. 2005 27 1 79-80. 11. J. 2005 30 11 1012-1013. 12. J. 2006 31 9 35-36. 13. J. 2000 31 10 764-765. 14 Kurihara H Sumio A Shibata H et al. Hypolipemic effect of Cyclocarya paliurus Batal Iljinskaja in lipid - loaded mice J. Biol Pharm Bull. 2003 26 3 383-385. 15 Yuan L J Tu D W Wu J P et al. Hypoglycemic and hypocholesterolemic effects of coptis chinensis franch inflorescence J. Plant Foods Human Nutr. 2006 61 139-144. 16 Lee H S Yoo C B Ku S K et al. Hypolipemic effect of water extracts of Picrorrhiza kurroa in high fat diet treated mouse J. Fitoterapia 2006 77 7-8 579-584. 17 Martinello F Soares S M Franco J J et al. Hypolipemic and antioxidant activities from Tamarindus indica L. pulp fruit extract in hypercholesterlolemic hamsters J. Food Chem Toxicol 2006 44 6 810-818. 18 Zaborska B Klos J Sikora - Frac M et al. Micronized fenofibrate decreased triglyceride levels total cholesterol and LDL fractions in serum J. Pol Arch Med Wewn 2000 104 1 371-375. 19 Juzwiak S Wojcicki J Mokrzycki K et al. Effect of quercetin on experimental hyperlipidemia and atherosclerosis in rabbits J. Pharmacol Rep 2005 57 5 604-609. 20 Sovova M Sova P. Pharmaceutical importance of Allium sativum L. hypolipemic effects in vitro and in vivo J. Ceska Slov Farm 2004 53 3 117-123. 21. J. 2002 178 4 466. 22. J. 1994 29 387-389. 23. M. 1994 311-312. 24. M. 2. 1994 1757-1767. 25. J. 2002 9 5 355-356. 26 Pulkowski G Fabisiak J. The effect of hypolipemic therapy with diet and simvastatin on the course of angina pectoris and the results of exercise stress test in patients with coronary artery disease J. Pol Arch Med Wewn 2005 114 2 746-754. 27 Buemi M Nostro L Crascì E et al. Statins in nephrotic syndrome a new weapon against tissue injury J. Med Res Rev 2005 25 6 587-609.