MONDAY, April 10, 2017 GEORGE M O BRIEN CENTER FOR ADVANCED RENAL MICROSCOPY AND ANALYSIS TENTATIVE COURSE SCHEDULE April 10 April14, 2017 7:30 am - 8:30 am BREAKFAST R2 Lobby R2 Lobby 8:30 am -9:20 am Overview: Advancing Nephrology through Intravital Imaging: Achievements and Challenges R2 101 Bruce Molitoris Indiana Center for Biological Microscopy 9:20 am -10:10 am Intravital Multiphoton Microscopy R2 101 Ken Dunn Indiana Center for Biological Microscopy 10:10 am - 10:20 am BREAK (10 minutes) R2 Lobby 10:20 am - 11:10 am Practical Intravital Imaging R2 101 Ruben Sandoval - Indiana Center for Biological Microscopy 11:10 am - 12:00 pm Use of an Abdominal Imaging Window for Intravital Microscopy Saskia Ellenbroek - Cancer Biophysics, Hubrecht Institute, Utrecht Netherlands R2 101 12:00 PM LUNCH R2 Lobby 12:30 pm - 1:00 pm Bruce Molitoris Introduction to Workshops R2 101 1:00 pm 2:45 pm Session 1 2:45 pm - 3:15 pm BREAK (30 minutes) 5:00pm - 6:00 pm ROUND TABLE R2 101 Bruce Molitoris Indiana Center for Biological Microscopy 6:00 pm - 7:00 pm DINNER R2 Lobby 7:00 pm - 10:00 pm Individual projects by arrangement R2 202
TUESDAY, April 11, 2017 7:30 am - 8:30 am BREAKFAST R2 Lobby 8:30 am - 9:20 am Large Scale 3D Tissue Imaging and Analysis R2 101 Tarek Ashkar - Indiana University 9:20 am - 10:10 am Use of Digital Image Analysis for Studies of Renal Physiology R2 101 Ed Delp Purdue University 10:10 am - 10:20 am BREAK (10 minutes) R2 Lobby 10:20 am - 11:10 am Fluorescent Proteins and Biosensors R2 101 Richard Day Indiana University 11:10 am - 12:00 pm Using Fluorescent Probes to Study Acute Kidney Injury R2 101 Pierre Dagher Indiana University 12:00-1 PM LUNCH R2 Lobby 1:00 pm 2:45pm Session 1 2:45 pm - 3:15 pm BREAK (30 minutes) 5:00pm - 6:00 pm PLENARY LECTURE R2 101 Membrane Remodeling through Membrane Trafficking in Live Animals Roberto Weigert NCI-CCR 6:00 pm - 7:00 pm DINNER R2 Lobby 7:00 pm - 10:00 pm Individual projects by arrangement R2 202
WEDNESDAY, April 12, 2017 7:30 am - 8:30 am BREAKFAST R2 Lobby Expending the Toolbox for Intravital Imaging of Kidney Using Gene Delivery R2 101 8:30 am - 9:20 am Techniques Robert Bacallao Indiana University 9:20 10:10 am Optimizing Microscope Design for Multiphoton Excitation Studies R2 101 Warren Zipfel - Cornell University 10:10 am - 10:20 am BREAK (30 minutes) R2 Lobby 10:20 am - 11:10 am 3D Visualization and Analysis R2 101 Jeffrey Clendenon - Aeon Imaging Forster Resonance Energy Transfer Microscopy and Fluorescence Lifetime 11:10 am - 12:00 pm Microscopy R2 101 Richard Day - Indiana University 12:00-1PM Lunch R2 Lobby 12:10-1:00pm (lecture during lunch) Applications of Micropuncture Techniques and Fluorescence Lifetime Imaging for Intravital Studies R2 101 Takashi Hato Indiana University 1:00 pm 2:45pm Session 1 2:45 pm - 3:15 pm BREAK (15 minutes) 5:00pm - 6:00 pm Round Table R2 101 6:00pm - 7:00 pm DINNER R2 Lobby
THURSDAY, April 13, 2017 7:30 am - 8:30 am BREAKFAST R2 Lobby 8:30 am - 9:20 am Using Fluorescent Probes to Study Tubule Metabolism R2 101 Tim Sutton Indiana University 9:20 am - 10:10 am Building Your Own 2-Photon Microscope: Challenges, Advantages and Limitations R2 101 Roberto Weigert NCI-CCR 11:10 am 12:00 pm Writing a Shared Instrumentation Grant R2 101 Ken Dunn Indiana University 12:00-1PM LUNCH R2 Lobby 1:00 pm 2:45 pm Session 1 2:45 pm - 3:15 pm BREAK (30 minutes) 6:00 pm - 9:00 pm DINNER FRIDAY, APRIL 14, 2017 Open to individual studies on the microscopes
2017 Demonstration sessions Monday Session 1 (1:00-2:45pm): Session 2 (3:15-5:00pm) : SPIM, Deep Tissue Clearing Seth, Tarek, SPIM(E243C) Ruben & Silvia, surgery-lab 251, imaging-e243b (Leica microscope) Intravital Mouse Kidney Imaging (Tie2 transgenic mouse) Henry, surgery- E259, imaging-209 (Olympus1 microscope) Abdominal Imaging Window - surgical procedure (movie presentation) SPIM, Deep Tissue Clearing Seth, Tarek, SPIM (E243C) Ruben & Silvia, surgery-lab 251, imaging- E243B (Leica microscope) Intravital Mouse Kidney Imaging (Tie2 transgenic mouse) Henry, surgery-e259, imaging-e209 (Olympus1 microscope) Abdominal Imaging Window-surgical procedure (movie presentation) Session 1 (1:00-2:45pm): Quantitative Image Analysis (ImageJ) Seth, R2-201 Tuesday Session 2 (3:15-5:00pm) : Quantitative Image Analysis (Image J) Seth, R2-201 Ruben & Silvia, surgery -Lab 251, imaging- E243B (Leica microscope) Intravital Mouse Kidney Imaging (Dendra2 photoactivation) Henry, Takashi, surgery -E259, imaging E209 (Olympus2 microscope) Abdominal Imaging Window - surgical procedure (movie presentation) Session 1 (1:00-2:45pm): In vivo gene transfer via subcapsular injection - GCamp6s imaging- Rat Kidney George, Ruben, surgery room -E243D, Imaging E243 B (Leica microscope) 2P intravital FLIM (mouse kidney metabolic state) Seth, Takashi, surgery- E243D, imaging-e209 (Olympus1) 3D Image Rendering Jeff Clendenon, R2-201 Intravital Imaging of Mouse Pancreas Henry, surgery- E259, imaging- E233C (Olympus2 microscope) Session 1 (1:00-2:45pm): 7:00-10:00 - individual sessions by arrangements Wednesday Ruben & Silvia, surgery-lab 251, imaging- E243B (Leica microscope) Intravital Mouse Kidney Imaging (Dendra2 photoactiation) Henry, Takashi, surgery -E259, imaging-e209 (Olympus2 microscope) Abdominal Imaging Window-surgical procedure (movie presentation) Session 2 (3:15-5:00pm) : In vivo gene transfer via subcapsular injection - GCamp6s imaging- Rat Kidney George, Ruben, surgery room -E243D, Imaging E243 B (Leica microscope) 3D Image Rendering Jeff Clendenon, R2-201 7:00-10:00 - individual sessions by arrangements Thursday Intravital Imaging of Mouse Liver Henry, surgery- E259, imaging- E233C (Olympus2 microscope) Session 2 (3:15-5:00pm) : Intravital Kidney Imaging on an Upright Microscope (microinjection) Henry and Amy, surgery-e243d, imaging-e243b (Leica microscope) 2P intravital FLIM (mouse kidney-sepsis) Seth, Takashi, surgery- E243D, imaging-e209 (Olympus1) Large Scale Cytometry Tarek, R2-201 1P FLIM in cultured cells Rich, MS 369 Intravital Kidney Imaging on an Upright Microscope (microinjection) Henry and Amy, surgery-e243d, imaging-e243b (Leica microscope) Large Scale Cytometry Tarek, R2-201 1P FLIM in cultured cells Rich, MS 369 Friday - sessions by arrangements