Rapid Screening of Milk & Other Dairy Samples IS09001 CERTIFIED

Rapid Screening of Milk & Other Dairy Samples IS09001 CERTIFIED

AuroFlow Strip Tests Antibiotic Residues in Raw Milk Works with room temperature or cold milk Results in just 7-10 minutes No required heating step Results easy to interpret visually or by use of a reader Ideal for use in the field or farm because they require no equipment Figure 1: Strip Test Protocol Pipet Milk into Sample Well. Mix Well Incubate at RT for 3 Minutes AuroFlow Beta-lactam Strip Test AuroFlow Tetracycline Strip Test AuroFlow Sulfonamide Strip Test AuroFlow Fluoroquinolones Strip Test Combo Kits: AuroFlow BT Combo StripTest AuroFlow BTS Combo Strip Test Insert Dipstick into Sample Well Allow Sample to Flow up Dipstick for 4 Minutes Read Results The AuroFlow lateral flow test strips are designed for the detection of a broad range of antibiotics in raw, commingled cow s milk. These rapid tests meet or exceed the sensitivities required to adhere to EU standards for each of the major tetracyclines (Table 1), 13 beta-lactam antibiotics (Table 2), 11 major sulfonamides (Table 3), and 8 fluoroquinolones (Table 4). The combo kits allow simultaneous detection of different classes of antibiotic residues. The BTS Combo kit can detect more antibiotics at or below the MRL, compared to any available competitor kit. These state-of-the-art tests also use novel binding proteins which eliminate the need for a heating block. Validation reports are available showing the efficacy of the tests in both room temperature and cold milk. The fast, simplified protocol can be seen in Figure 1. Simple Interpretation of Results When the signal at the T-line is more intense than the signal at the C-line, the milk sample is negative for presence of residues. A signal at the T-line which is equal to or less intense than the signal at the C-line indicates a contaminated milk sample. AuroFlow BT Combo Strip Test Strip Orientation & Interpretation of Results Negative ß-lactam Positive Tetracycline Positive ß-lactam & Tetracycline Positive Table 1: MRLs for Tetracycline Antibiotics in Milk & Detection Limits of the AuroFlow Tetracycline, BT Combo & BTS Combo Strip Tests Substance MRL AuroFlow Tetracycline 100 50-100 Oxytetracycline 100 50-70 Chlorotetracycline 100 15-50 Doxycycline 100 5-20 C-Line ß-lactam Tetracycline

Table 2: MRLs for Beta-lactam Antibiotics in Milk and Detection Limits of the AuroFlow Beta-lactam and BT Combo Strip Tests and Competitive Strip Tests. Substance MRL AuroFlow Competitor S Competitor TS Competitor R Competitor BS Penicillin G 4 3 3 3 3 3 Ampicillin 4 4 6 4 4 4 Amoxicillin 4 4 7 4 4 4 Oxacillin 30 4 40 15 40 6 Cloxacillin 30 5 35 8 35 6 Dicloxacillin 30 4 25 8 35 5 Nafcillin 30 15 80 35 60 14 Cephquinome 20 15 20 30 20 10 Ceftiofur 100 75 12 13 20 110 Cefazolin 50 30 15 23 20 60 Cephapirin 60 6 12 7 10 12 Cephalonium 10 4 4 3 5 4 Cefaperazone 50 6 7 3 9 6 Table 3: Detection Limits for Sulfonamide Antibiotics Sulfonamide Antibiotics Antibiotic (MRL) Detection Limit (µg/l) Sulfmethazine (100) 25-50 ppb Sulfamethoxazole (100) 25-50 ppb Sulfadiazine (100) 50-75 ppb Sulfathiazole (100) 75-100 ppb Sulfadimethoxine (100) 50-75 ppb Sulfaquinoxaline (100) 75-100 ppb Sulfapyridine (100) 2-4 ppb Sulfamethoxypyridazine (100) 3-4 ppb Sulfamethizole (100) 40-50 ppb Sulfamerazine (100) 25-50 ppb Sulfachloropyridazine (100) 25-50 ppb Table 4: Detection Limit by Visual Interpretation Method for Major Fluoroquinolone Antibiotics Antibiotic (MRL) Enrofloxacin (100) Ciprofloxacin (100) Difloxacin Sarafloxacin Norfloxacin Marbofloxacin (75) Danofloxacin (30) Flumequine (50) Detection Limit (µg/l) 1.5-2.5 ppb 1-2 ppb 20-30 ppb 20-30 ppb 1-2 ppb 4-6 ppb 2-3 ppb 4-6 ppb AuroFlow Aflatoxin M1 Strip Test The AuroFlow Aflatoxin M1 Strip Test Kit is a qualitative and rapid lateral flow assay designed to detect Aflatoxin M1 residues in raw commingled cow s milk at 0.5 ppb in 10 minutes. 1067-01; -02 AuroFlow Beta-lactam Strip Test Kit Strip Test 96; 192 1088-01; -02 AuroFlow Tetracycline Strip Test Kit Strip Test 96; 192 1111-01; -02 AuroFlow Sulfonamide Strip Test Kit Strip Test 96; 192 1102-01; -02 AuroFlow Fluoroquinolone Strip Test Kit Strip Test 96; 192 1087-01; -02 AuroFlow BT Combo Strip Test Kit Strip Test 96; 192 1116-01; -02 AuroFlow BTS Combo Strip Test Kit Strip Test 96; 192 1101-01; -02 AuroFlow Aflatoxin M1 Strip Test Kit Strip Test 96; 192

AuroFlow Melamine Strip Test Kit Works with room temperature or cold milk Fast 7 minute protocol No sample preparation required Easy two-step assay Most sensitive lateral flow assay with a sensitivity of 50 ppb The AuroFlow Melamine Strip Test Kit is a qualitative and rapid lateral flow assay designed to detect melamine residues in raw, commingled cow s milk and reconstituted milk powder. This simple, two-step test requires no sample preparation and is designed for rapid field use or reference lab settings. Using the Detekt RDS-Reader (see page 30) melamine can be detected at 50 ppb; visually 100 ppb can be detected. Detekt RDS-1000 Strip Reader Increase testing throughput and efficiency with the ability to automatically test > 300 assays per hour with a single unit Eliminate costly false positives and negatives by automatically determining results Archive and track test results trends over time to improve QA/QC and meet new government testing standards Reduce retesting, supply and reagent waste The Detekt RDS-1000 Strip Reader is a patented ruggedized handheld lateral flow testing system that provides rapid quantitative assay results and increases testing throughput and efficiency. The unit features a rechargeable lithium battery, a touchscreen menu driven interface, and provides users test results in less than 10 seconds. The unit is compatible with a wide array of pre-programmed test types and is software upgradeable to accommodate additional assays. All results including the actual test and control line images are saved and can be automatically transferred to a desktop computer using the supplied Data Manager Software Suite. This Strip Reader has been approved for use with all of Bioo Scientific s AuroFlow Strip Tests. Columns AflaPure M1 Aflatoxin Column Kit Flexible - For use with fluorescence-based HPLC detection and ELISA Fast - Sample ready in less than 30 minutes Robust dynamic range - Detects aflatoxin M1 levels from 10 ppt - 3 ppb Safe - Uses nontoxic materials for sample prep protocols The AflaPure M1 Aflatoxin Column Kit contains immunoaffinity columns for the convenient purification and enrichment of aflatoxin M1 from milk samples for downstream HPLC and ELISA analysis. Aflatoxin M1 is a naturally occurring fungal toxin sometimes present in milk samples. Contamination of milk with aflatoxin can cause a variety of illnesses including liver cancer. AflaPure M1 Aflatoxin Rapid Column Kit Designed for use with direct fluorometric detection of M1 aflatoxin in milk Fast - Sample ready in less than 7 minutes Sensitive - The limit of detection is 0.05 ppb Safe - Uses nontoxic materials for sample prep protocols The AflaPure M1 Aflatoxin Column Kit contains immunoaffinity columns for the convenient purification and enrichment of aflatoxin M1 from milk samples for downstream HPLC and ELISA analysis. Aflatoxin M1 is a naturally occurring fungal toxin sometimes present in milk samples. Contamination of milk with aflatoxin can cause a variety of illnesses including liver cancer. 1091-01; -02 AuroFlow Melamine Strip Test Kit Strip Test 96; 192 1952-01 Detekt RDS-1000 Strip Reader - - 1402-01 AflaPure M1 Aflatoxin Column Kit Column 25 Columns 1404-01 AflaPure M1 Aflatoxin Rapid Column Kit Column 25 Columns

Enzymatic Assays MaxSignal Total Antibiotics in Milk Test Kit Detection of wide range of antibiotics in milk A quick spore growth assay Fast results As little as 3 hours from sample to results Easy, high recovery sample preparation Consistent, reproducible results The MaxSignal Total Antibiotics in Milk Test Kit provides an easy and simple test method for detecting total antibiotics in milk. The MaxSignal Total Antibiotics in Milk Test Kit is more rapid, easier to use and more sensitive than any other commercially available kit for the detection of total antibiotics in milk samples MaxSignal Histamine Enzymatic Assay Kit Consistent, reproducible results Simple - Add samples and master mix to plate Fast results As little as 15 minutes from sample prep to results Elevated levels of histamine can cause allergic reactions and/or scromboid poisoning. The MaxSignal Histamine Enzymatic Assay Kit, based on Bioo Scientific s proprietary technology, is a colorimetric enzymatic assay for the determination of histamine levels in seafood, meat, milk, and wine. This kit is ideal for rapid, high throughput detection of unsafe levels of histamine in food products, saving money and time compared to other screening methods. A new methanol-free sample prep protocol is available. MaxSignal Beta-lactamase Test Pad Kit Rapid - Requires less than 20 minutes High reproducibility 0.8 U/mL detection limit The MaxSignal Beta-Lactamase Test Pad Kit is ideal for the routine screening of beta-lactamase in milk. This sensitive kit enables government agencies, food manufacturers and quality assurance organizations to detect beta-lactamase contamination as low as 0.8 U/mL. MaxSignal No-Mel Milk Test Kit Rapid and simple method Employs a modified version of the AOAC method Not influenced by melamine in test samples This rapid microwell test determines the concentration of protein in milk and other milk products such as cream, ice cream, powered milk, and chocolate drinks. The assay detects the specific binding of milk proteins to dye molecules. This detection is fundamentally different than that of some other protein determination methods (such as the Kjeldahl method) which detect protein via nitrogen and are vulnerable to contamination of amine containing impurities such as melamine. The MaxSignal No-Mel Milk Total Protein Assay Kit provides accurate, proven results even in the presence of high levels of melamine. 1023-01; -02 MaxSignal Total Antibiotics in Milk Test Kit Biological Assay 1 x 96; 3 x 96 Wells 1032-04 MaxSignal Histamine Enzymatic Assay Kit Enzymatic Assay 1 x 96 Wells 1061-02 MaxSignal Beta-lactamase Test Pad Kit Pad-based 50 Samples 1050-01 MaxSignal No-Mel Milk Test Kit Dye Binding 1 x 96 Wells

MaxSignal Urea Enzyme Assay Test Kit Used for serum and dairy testing Minimal sample prep for milk Sensitive enough to detect 20 ppm of urea The MaxSignal Urea Enzyme Assay Test is a practical choice for regulatory agencies and food processors to determine urea levels in dairy products, allowing them to readily address the consumers concerns about food integrity. The assay utilizes urease, a metabolic enzyme, to specifically detect urea in fluids. The MaxSignal Urea Enzymatic Assay Kit provides rapid, accurate, proven results even in complex liquid mixtures. The limit for detection is 20 ppm urea in milk samples. The linear range of the assay is 20-100 ppm of analyte. MaxSignal Dairy Product Pasteurization Verification Kit Minimal sample preparation High accuracy and high reproducibility The MaxSignal Dairy Product Pasteurization Verification Kit is a colorimetric enzyme assay that confirms the pasteurization of milk, cheese, and dairy products. Milk contains readily detectable levels of an enzyme called alkaline phosphatase. The inactivation of milk alkaline phosphatase occurs at a higher temperature threshold than the threshold for pasteurization. Therefore observation of loss of alkaline phosphatase activity in heat-treated milk samples indicates that pasteurization has occurred. As such, inactivation of milk alkaline phosphatase is the now the universally accepted indicator for successful pasteurization of dairy products. This simple enzyme-based assay determines the inactivation of alkaline phosphatases in testing samples. ELISA Kits MaxSignal Aflatoxin M1 ELISA Test Kit New rapid and sensitive versions available Extraction-free milk analysis Recovery rates greater than 80% Bioo Scientific has added to its line of simple, quick, and fully quantitative ELISAs for mycotoxins with the introduction of two new MaxSignal Aflatoxin M1 ELISA Kits; one with increased speed and other with increased sensitivity. Both of these kits are designed to simplify the analysis of dairy products for aflatoxin M1 contamination. The sensitive version of the MaxSignal Aflatoxin M1 ELISA Kit (Cat # 1060-05) offers sensitivity of 5 ppt (0.005 ppb) directly in milk samples. The rapid version (Cat # 1060-02) can be completed in only 30 minutes offering a sensitivity of 100 ppt (0.1 ppb) for milk and cheese samples. Both of these kits do not require any extraction process for milk samples. MaxSignal Melamine ELISA Test Kit Consistent, reproducible results Offers 4-fold greater sensitivity than the competition Easy, high recovery sample preparation Includes robust sample prep protocols for chocolate and milk The MaxSignal Melamine ELISA Kit offers significant improvements over other melamine ELISAs. It has been optimized to offer a very low background and can be used to detect melamine contamination at concentrations as low as 0.2 ppm. This kit also offers unique and robust sample preparation protocols for milk, milk powder, feed, meat and even difficult sample types, such as chocolate, while offering recovery rates of 80% or more. The MaxSignal Melamine ELISA Kit was designed to be the most sensitive ELISA available and is ideal for the rapid screening of food and feed samples for melamine contamination. 1051-01 MaxSignal Urea Enzyme Assay Test Kit Enzymatic Assay 1 x 96 Wells 1041-01 MaxSignal Dairy Product Pasteurization Verification Kit Enzymatic Assay 60 Reaction Tubes 1060-05 MaxSignal Aflatoxin M1 ELISA Test Kit ELISA 1 x 96 Wells 1077-01 MaxSignal Melamine ELISA Test Kit ELISA 1 x 96 Wells

MaxSignal Tetracycline ELISA Test Kit Most sensitive kit available Consistent, reproducible results Rapid, column-free meat extraction protocol With a detection limit of 0.5 ppb, the milk version of the MaxSignal Tetracycline ELISA Test Kit (Cat. # 1016-03) offers more sensitive quantitation of tetracycline residues in milk than any other competing ELISA. This increased sensitivity makes the kit ideal for customers who need to ensure that their dairy products are free of tetracycline contamination. The method is based on a competitive colorimetric ELISA assay. Related Dairy MaxSignal ELISA Kits Bioo Scientific has introduced a complete line of easy-to-use, economical, rapid antibiotic ELISAs that allow our customers to meet regulatory requirements and provide consumers with safe, quality milk and dairy products. For more information about any of these kits ask your distributor or contact Bioo Scientific directly at info@biooscientific.com. 1014-01 MaxSignal Streptomycin ELISA Test Kit ELISA 1 x 96 Wells 1026-01 MaxSignal Tylosin ELISA Test Kit ELISA 1 x 96 Wells 1013-04 MaxSignal Chloramphenicol (CAP) ELISA Kit ELISA 1 x 96 Wells 1065-01 MaxSignal Beta-Lactam ELISA Kit ELISA 1 x 96 Wells 1014-01 MaxSignal Gentamicin ELISA Kit ELISA 1 x 96 Wells 1056-01 MaxSignal Sulfonamide ELISA Kit ELISA 1 x 96 Wells 1084-01 MaxSignal Ceftiofur ELISA Kit ELISA 1 x 96 Wells 1024-01 MaxSignal Fluoroquinolone ELISA Kit ELISA 1 x 96 Wells 1068-01 MaxSignal Ciprofloxacin ELISA Test Kit ELISA 1 x 96 Wells 1017-01 MaxSignal Enrofloxacin ELISA Test Kit ELISA 1 x 96 Wells 1058-01 MaxSignal Norfloxacin ELISA Test Kit ELISA 1 x 96 Wells 1059-01 MaxSignal Flumequine ELISA Test Kit ELISA 1 x 96 Wells 1020-03 MaxSignal Furaltadone (AMOZ) ELISA Test Kit ELISA 1 x 96 Wells 1015-02 MaxSignal Furazolidone (AOZ) ELISA Test Kit ELISA 1 x 96 Wells 1070-01 MaxSignal Nitrofurantoin (AHD) ELISA Test Kit ELISA 1 x 96 Wells 1069-01 MaxSignal Nitrofurazone (SEM) ELISA Test Kit ELISA 1 x 96 Wells 1012-01 MaxSignal Diethylstibestrol (DES) ELISA Kit ELISA 1 x 96 Wells Reliable Kits Bioo Scientific understands the importance of having a rapid, reliable and accurate method for the detection of residues in milk. All of Bioo Scientific s kits undergo rigorous testing to ensure that they are robust and easy to use. In addition, to assure quality, Bioo Scientific s quality management system has been certified to the international standards ISO 9001:2008.