Journl of Applied nd Nturl Science 10 (1): 448-453 (2018) A comprtive study of Zn nd Fe distriution in two contrsting whet genotypes Bhupendr Mthpl 1*, Prksh Chndr Srivstv 2 nd Shilesh Chndr Shnkhdhr 3 1 School of Agriculture, Lovely Professionl University, Phgwr -144411(Punj), INDIA 2 Deprtment of Soil Science, College of Agriculture, G. B. Pnt University of Agriculture nd Technology, Pntngr-263145 (Uttrkhnd), INDIA 3 Deprtment of Plnt Physiology, College of Bsic Sciences nd Humnities, G. B. Pnt University of Agriculture nd Technology, Pntngr-263145 (Uttrkhnd), INDIA *Corresponding uthor. E-mil: hupendr.20525@lpu.co.in Received: Octoer 23, 2017; Revised received: Decemer 6, 2017; Accepted: Ferury 20, 2018 Astrct: Effect of zinc nd iron interction on their distriution ws exmined in two whet genotypes (UP262 nd UP2628) under folir ppliction of 0, 0.25 nd 0.50% ZnSO 4 solution tgged with 925 KBq of Zn 65 pot -1 for Zn nd 0, 0.5 nd 1.0% FeSO 4 solution tgged with 925 KBq of Fe 59 pot -1 for Fe t 30, 60 nd 90 dys fter plnting. Mximum grin yield of UP2628 (2.7 g pot -1 ) ws recorded t 0.5%ZnSO 4 +0%FeSO 4 while tht of UP262 (2.63 g pot -1 ) ws recorded t 0.5%ZnSO 4 +1.0%FeSO 4. The highest strw yield of UP2628 (2.75 g pot -1 ) ws noted t 0.5% ZnSO 4 +1.0%FeSO 4 while tht of UP262 (2.91 g pot -1 ) with 0.5%ZnSO 4 +0.5%FeSO 4. Appliction of 0.5% nd 1.0% FeSO 4 reduced the ccumultion of 65 Zn in ll prts of oth the vrieties. Regrding the 59 Fe ccumultion, it ws found to e decresed with the incresed ppliction of ZnSO 4 solution from 0.25% nd 0.5% s compred to without ppliction of Zn. On compring trnsloction efficiencies of oth the vrieties, UP2628 showed etter trnsloction thus ccumulted higher zinc nd iron. Therefore, vriety UP2628 cn e used further for crop improvement progrmme. Keywords: Folir ppliction, Fe, Trnsloction, Whet, Zn INTRODUCTION Humns require t lest 49 nutrients to meet their metolic requirements (Prksh et l., 2016). Indequte consumption of even single of these nutrient results in dverse metolic disturnces such s sickness, poor helth, impired development in children, etc. (Welch nd Grhm, 2004). Popultions residing in developing countries consume cerels s primry food components. Poor grin nutritive vlue of cerels is n importnt reson for widespred micronutrient mlnutrition mong popultions eting rice s stple food (Chndel et l., 2010). Besides, the plnt-derived food contins wide vriety of minerls tht my hve eneficil or detrimentl effects on humn helth nd well eing. Of the 17 micronutrients, the deficiencies of iron (Fe) nd zinc (Zn) lone in humn foods ffect lrge proportion of the world s popultion (Shhzd et l., 2014). The key resons for humn minerl mlnutrition re the reltively low content of Fe nd Zn in plnt sed foods in comintion with the undnce of ntinutrient compounds tht severely reduce their iovilility (Ghndilyn et l., 2006). The min soil fctors responsile for cusing micronutrients deficiency in stple food crops re low content ISSN : 0974-9411 (Print), 2231-5209 (Online) journls.nsfoundtion.org of nutrient itself, soil ph, high content of clcite, high concentrtion of icronte ions nd slts, high level of ville phosphorus nd interction with nother nutrient element (Mthpl et l., 2015). The criticl vlue of Zn to occur its deficiency in soil ws found in the rnge of 0.6 ppm to 2.0 ppm depending upon extrction method (Singh et l., 2005) while for Fe it ws reported 3.4 mg kg -1 (Elgl et l., 2008). Interctions mong micronutrients ffect their uptke, distriution, nd utiliztion in plnts (Imtiz et l., 2003). Mny studies hve exmined these interctive effects, especilly etween Fe nd Zn. These studies showed ntgonism etween Fe nd Zn in soyen (Slimn, 1990). Other studies reported tht Fe reduces Mn concentrtion in Indin mustrd (Hmlin et l., 2008) nd in soyen leves (Izguirre-Myorl et l., 2005), ut increses Mn concentrtion in soyen shoots (Heenn nd Cmpell, 1983). Other studies reported negtive correltion etween Zn nd Cu (Kumr et l., 2009) nd negtive correltion ws reported etween Zn nd Fe in whet (Ai-Qing et l., 2011). As cerels re the mjor food source for humns, therefore n ttempt hs een mde through present investigtion to evlute the trnsloction pttern nd ccumultion of 65 Zn nd 59 Fe in two contrsting This work is licensed under Attriution-Non Commercil 4.0 Interntionl (CC BY-NC 4.0). 2018: Author (s). Pulishing rights @ ANSF.
Fe conc. in grins ( g g -1 ) Zn conc. in grins ( g g -1 ) Bhupendr Mthpl et l. / J. Appl. & Nt. Sci. 10 (1): 448-453 (2018) whet genotypes. MATERIALS AND METHODS A ulk surfce (0-15 cm) soil (Typic Hpludoll) ws collected y squre method from E1 plot of Normn E. Borluge Crop Reserch Center, G. B. Pnt University of Agriculture nd Technology, Pntngr, Indi. The experimentl soil hd sndy lom texture, 7.4 ph nd 0.266 dsm -1 E.C., 10.5 g orgnic C, 0.47 mg DTPA extrctle Zn kg -1 soil nd 25.3 mg DTPA extrctle Fe kg -1 soil. The seeds of two contrsting genotypes of whet viz. UP262 (Zn inefficient) nd UP2628 (Zn efficient) were otined from the Deprtment of Genetics nd Plnt Breeding of the University. The soil ws ir dried nd crushed with wooden roller nd pssed through sieve hving openings of 2 mm dimeter. The soil ws filled in plstic pots of 4 kg cpcity. A sl dose of nitrogen, phosphorus nd potssium were pplied to ll pots t the rte of 22.3 mg N, 11.6 mg P nd 18.5 mg K kg -1 soil using stock solutions of ure, KH 2 PO 4 nd KCl, respectively. Five seedlings were plnted in ech pot. The folir spry tretments consisted of fctoril comintion of 0, 0.25, 0.5% ZnSO 4 solution tgged with 925 KBq of 65 Zn pot -1 nd 0, 0.5, 1.0% FeSO 4 solution tgged with 925 KBq of 59 Fe pot -1 t 30 (tillering), 60 (pnicle initition) nd 90 (grin filling) dys fter sowing in triplicte. For ech folir spry of Zn nd Fe solution, 10 ml volume ws used per pot. The experimentl design ws three fctoril completely rndomized designs. At mturity (115-120 dys fter sowing), the plnts were hrvested, nd the eril prts of the plnt were prtitioned into leves, stem nd grins. Plnt smples were wshed with tp wter, 0.1N HCl nd susequently with distilled wter to remove the surfce contmintion. Then the smples were dried, nd 1g of ech plnt prt ws digested in di-cid (HNO 3 : HClO 4 in 10:4 v/v) (Pirzdeh et l., 2010) nd finl volume ws mde upto 14 ml with doule distilled wter. Two ml liquot of the digested smple ws used to ssy ctivities of 65 Zn nd 59 Fe on gmm ry spectrometer with NI crystl (model GRS 101P). Activity ws expressed s cpm g -1 dry weight of plnt smple. After recording the ctivities finl volume of this two ml liquot ws mde 10 ml nd concentrtion of Zn nd Fe ws recorded in grins y tomic sorption spectrophotometer (AAS, ECIL, Hyderd Indi). The experiment ws crried out in three fctoril completely rndomized design nd sttisticl nlysis of ll the prmeters ws done y using nlysis of vrince (ANOVA) using SPSS 16 (Bristol, UK). The mens were tested t the P 0.05 level of significnce. RESULTS AND DISCUSSION Counts per minute of 65 Zn nd 59 Fe: The counts per 40 30 20 10 0 0Zn,0Fe 0Zn,0.5Fe 0Zn,1Fe 0.25Zn,0Fe 0.25Zn,0.5Fe 0.25Zn,1Fe Tretments 0.5Zn,0Fe 0.5Zn,0.5Fe 0.5Zn,1Fe minute of 65 Zn in leves, stem nd grin of two whet genotypes t mturity re presented in Tle 1. The vrieties were significntly different in the ccumultion of 65 Zn in different plnt prts nd UP2628 ccumulted more 65 Zn (10% more in lef, 26.8% more in the stem nd 35.7% more in grin) s compred to UP262. Similrly, Zn levels lso influenced the ccumultion of 65 Zn significntly, nd it ws found improved in ll the plnt prts with incresing Zn levels. The ppliction of 0.5% Zn sulphte solution incresed the 65 Zn ccumultion y 5.6% in leves, 16.7% in the stem nd 7.4% in grins s compred to 0.25% Zn sulphte solution. The ppliction of incresing levels of ferrous sulphte decresed the ccumultion of 65 Zn in ll the plnt prts except the stem. The ppliction of 0.5% nd 1.0% ferrous sulphte solution decresed the ccumultion of 65 Zn in leves y 12.7 nd 14.9%, in grins y 1.7 nd 3.1% respectively, s compred to 0 level of ferrous sulphte solution. In stem somewht UP2628 UP 262 Fig. 1. Effect of folirly pplied zinc nd iron levels on zinc concentrtion (µg g -1 ) in grins of two contrsting whet genotypes. 180 150 120 90 60 30 0 e e 0Zn,0Fe 0.25Zn,0Fe c f d d 0.5Zn,0Fe 0Zn,0.5Fe 0.25Zn,0.5Fe c e cd 0.5Zn,0.5Fe 0Zn,1Fe Tretments d 0.25Zn,1Fe 0.5Zn,1Fe UP2628 UP 262 Fig 2. Effect of folirly pplied zinc nd iron levels on iron concentrtion (µg g -1 ) in grins of two contrsting whet genotypes. 449
Bhupendr Mthpl et l. / J. Appl. & Nt. Sci. 10 (1): 448-453 (2018) Tle 1. Effect of different levels of zinc nd iron on distriution of 65 Zn (counts per minute) in different plnt prts of two contrsting whet genotypes. A. Leves UP2628 UP262 Zn х Fe interction 0.25 0.50 Av. 0.25 0.50 Av. 0.25 0.50 Av. 0.0 10219 10344 10281 9844 10969 10406 10031 10656 10343 0.5 9375 10438 9905 6906 9375 8140 8141 9906 9023 1.0 8938 10000 9469 9500 6750 8125 9219 8375 8797 Av. 9510 10260 9885 8750 9031 8890 9130 9645 9387 S.Em. ± 81 81 99 115 141 141 199 LSD t 0.05 197 197 241 NS 342 342 483 B. Stem UP2628 UP262 Zn х Fe interction 0.25 0.50 Av. 0.25 0.50 Av. 0.25 0.50 Av. 0.0 2079 2723 2401 1449 2069 1759 1764 2396 2080 0.5 2636 2342 2489 1736 1733 1734 2186 2037 2111 1.0 1922 2454 2188 1484 1883 1683 1703 2168 1935 Av. 2212 2506 2359 1556 1895 1725 1884 2200 2042 S.Em. ± 18 18 22 25 31 31 44 LSD t 0.05 44 44 54 NS 76 76 108 C. Grins UP2628 UP262 Zn х Fe interction 0.25 0.50 Av. 0.25 0.50 Av. 0.25 0.50 Av. 0.0 1040 1197 1118 672 767 719 856 982 919 0.5 1064 1103 1083 711 735 723 887 919 903 1.0 1054 1145 1099 690 672 681 872 908 890 Av. 1052 1148 1100 691 724 707 871 936 903 S.Em. ± 7 7 9 10 13 13 18 LSD t 0.05 18 18 NS 26 32 NS NS unexpected results were recorded, where the ppliction of 0.5% ferrous sulphte incresed the 65 Zn ccumultion y 1.4% while further reduction of 6.9% ws found y the ppliction of 1.0% level of ferrous sulphte. The interction effect of vriety Zn Fe influenced the ccumultion of 65 Zn in leves nd stem significntly however no significnt effect ws oserved in the grins. Similrly, folirly pplied solutions of 0.25% nd 0.5% Zn sulphte t 0.5% ferrous sulphte solution incresed the ccumultion of 65 Zn in leves nd grins while slight reduction ws recorded in stem. Folir spry of 0.25% nd 0.5% Zn sulphte solution t 1.0% ferrous sulphte enhnced the ccumultion of 65 Zn in ll the plnt prts of UP2628 ut in UP262 these were found effective in improving the 65 Zn in stem only. Accumultion of 59 Fe in leves, stem nd grin of oth whet vrieties ws significntly ffected y different ferrous sulphte levels (Tle 2). Regrding the vriety, UP2628 ccumulted higher 59 Fe (6.5% in lef, 7.5% in the stem nd 16% in grin) s compred to UP262. The ccumultion of 59 Fe incresed with the increse in Fe concentrtion from 0.5% to 1.0% ferrous sulphte y 5.2 percent in the stem, y 3.9 percent 450 in grins for oth the vrieties except in the leves where the ccumultion ws reduced t 1.0% ferrous sulphte solution s compred to 0.5% ferrous sulphte. However, the min effect of Zn levels ws found to e sttisticlly non-significnt on 59 Fe ccumultion. The interction effect of vriety Zn Fe significntly influenced the 59 Fe ccumultion in stem while the effect ws sttisticlly not significnt in leves nd grins of oth the vrieties. After compring oth the vrieties under ll the levels of Zn nd Fe, it ws depicted tht t 0% level of zinc sulphte, folir spry of 0.5% nd 1.0% ferrous sulphte solution incresed the ccumultion of 59 Fe in ll the plnt prts except in leves of UP262. Similrly, t 0.25% zinc sulphte solution the folir ppliction of 0.5% nd 1.0% ferrous sulphte solution incresed the 59 Fe ccumultion in ll the plnt prts except in leves of UP262. The folir ppliction of 0.5% nd 1.0% ferrous sulphte t 0.5% zinc sulphte level solution enhnced the ccumultion of 59 Fe in stem nd grins ut reduced in the leves of oth the vrieties. The results clerly indicted tht UP2628 ccumulted higher Zn in ll the plnt prts in comprison to UP262. Therefore, it could e concluded tht vriety
Bhupendr Mthpl et l. / J. Appl. & Nt. Sci. 10 (1): 448-453 (2018) Tle 2. Effect of different levels of zinc nd iron on distriution of 59 Fe (counts per minute) in different plnt prts of two contrsting whet genotypes. A. Leves UP2628 UP262 Zn х Fe interction 0.50 2798 2776 2713 2762 2760 2726 2713 2733 2779 2751 2713 2747 1.00 2820 2810 2670 2766 2466 2364 2479 2436 2643 2587 2574 2601 Av. 2809 2793 2691 2764 2613 2545 2596 2584 2711 4044 2643 3132 S.Em. ± 33 40 33 57 47 57 81 LSD t 0.05 80 NS 80 NS NS 139 NS B. Stem UP2628 UP262 Zn х Fe interction 0.50 831 805 789 808 742 745 770 752 786 775 779 780 1.00 842 860 869 857 771 803 785 786 806 832 827 821 Av. 836 832 829 832 756 774 777 769 796 803 803 800 S.Em. ± 1.8 2.2 1.8 3.1 2.5 3.1 4.4 LSD t 0.05 4.3 NS 4.3 7.6 6.2 7.6 10.7 C. Grin UP2628 UP262 Zn х Fe interction 0.50 247 248 253 249 212 211 214 212 229 229 233 230 1.00 274 262 257 264 219 213 210 214 247 237 234 239 Av. 260 255 255 256 215 212 212 213 238 233 233 234 S.Em. ± 2.1 2.6 2.1 3.7 3.0 3.7 5.3 LSD t 0.05 5.2 NS 5.2 NS NS 9.1 NS UP2628 showed etter uptke s well s trnsloction efficiency thn UP262. Accumultion of Zn ws found to increse with incresing zinc sulphte levels, ut the incresing ferrous sulphte levels showed n ntgonistic effect in ll the plnt prts except in stem of UP2628 nd UP262. Like in cse of 65 Zn ccumultion, UP2628 ccumulted more 59 Fe in ll the plnt prts s compred to UP262. The ccumultion of Fe ws incresed with incresing levels of ferrous sulphte in ll the plnt prts except tht in leves of UP262. The incresing levels of zinc sulphte reduced the 59 Fe ccumultion in leves, stem nd grins of oth whet vrieties except in the stem of UP262. The reduced ccumultion of 59 Fe in different plnt prts under vrying levels of Zn nd vice vers might e due to the ntgonistic interction etween oth the elements t the uptke site nd long the long distnce trnsport (Allowy, 2008). It ws reported tht ppliction of 8 mg Fe kg -1 soil decresed the uptke of Zn y 31.9% in whet in comprison to its no ppliction (Ghsemi-Fsei nd Ronghi, 2008). Similrly, it ws lso shown y (Ai-Qing et l., 2011) tht n increse in Fe supply from 0 ppm to 5 ppm reduced the Zn content in stem nd leves of whet plnt y fctor of one-fifth to two-fifths while reduction in Fe ccumultion in different plnt prts of whet ws lso recorded with the ppliction of 10 ppm of Zn s compred 451 to 0 ppm. High concentrtion of Fe in soil ws lso found to reduce uptke nd trnsloction of Zn in different crop plnts (Ds, 2014). Likewise, ove mentioned results lso find support of the study done y (Chilin et l., 2015), who reported tht incresing levels of Zn were lso found to reduce ccumultion of Fe in different plnt prts of corn. Grin yield nd strw yield: The min effect of zinc levels nd interction effect of vriety iron on grin yield ws found to e sttisticlly significnt (Tle 3). The folir ppliction of 0.25% nd 0.5% zinc sulphte solution incresed the men grin yield of oth the whet vrieties y 1.7 nd 4.9 percent over no ppliction of zinc sulphte respectively. The ppliction of 0.5% nd 1.0% ferrous sulphte solution incresed the grin yield y 0.92 nd 11.5 percent in UP262 while in UP2628, 0.5% level of ferrous sulphte incresed the grin yield y 2.5 percent ut t higher level (1.0%) of ferrous sulphte reduction of 1.7 percent ws oserved s compred to no ppliction of ferrous sulphte. Overll, the mximum (2.70 g pot -1 ) grin yield ws recorded t 0.5%Zn+0%Fe for UP2628 while the minimum (1.86 g pot -1 ) ws lso recorded for UP2628 t 0%Zn+0%Fe. Zinc nd iron significntly ffect the strw yield of oth whet vrieties (tle 4). Folir ppliction of 0.25% nd 0.5% level of zinc sulphte incresed the
Bhupendr Mthpl et l. / J. Appl. & Nt. Sci. 10 (1): 448-453 (2018) Tle 3. Effect of different levels of zinc nd iron on grin yield (g pot -1 ) of two contrsting whet genotypes. UP2628 UP262 Zn х Fe interction 0.0 1.86 2.39 2.70 2.31 1.98 2.08 2.45 2.17 1.92 2.23 2.57 2.24 0.5 2.02 2.46 2.64 2.37 1.91 2.31 2.35 2.19 1.97 2.39 2.50 2.28 1.0 1.94 2.38 2.51 2.27 2.16 2.48 2.63 2.42 2.05 2.43 2.57 2.35 Av. 1.94 2.41 2.61 2.32 2.01 2.29 2.47 2.25 1.98 2.35 2.54 2.29 S.Em. ± 0.03 0.04 0.04 0.06 0.06 0.07 0.10 CD t 5% NS 0.12 NS NS NS 0.21 NS Tle 4. Effect of different levels of zinc nd iron on strw yield (g pot -1 ) of two contrsting whet genotypes. UP2628 UP262 Zn х Fe interction 0.0 2.34 2.43 2.52 2.43 2.07 2.59 2.71 2.45 2.20 2.51 2.61 2.44 0.5 2.35 2.50 2.74 2.53 2.31 2.50 2.91 2.57 2.33 2.50 2.83 2.55 1.0 2.31 2.53 2.75 2.47 2.37 2.56 2.82 2.58 2.34 2.55 2.79 2.56 Av. 2.33 2.48 2.67 2.49 2.25 2.55 2.81 2.53 2.29 2.52 2.74 2.51 S.Em. ± 0.03 0.03 0.03 0.05 0.05 0.06 0.09 CD t 5% NS 0.10 0.10 NS NS NS NS men strw yield of whet vrieties y 4.5 nd 4.9 percent respectively over no ppliction of zinc sulphte. Similrly, the folir ppliction of 0.5% nd 1.0% ferrous sulphte solution lso incresed the strw yield y 10.0 nd 19.6 percent s compred to no ppliction of ferrous sulphte solution, respectively. In generl, the highest strw yield ws recorded in UP262 under 0.5%Zn+0.5% Fe levels of zinc nd iron wheres the lowest ws lso recorded in UP262 t 0%Zn+0%Fe level. Sttisticl nlysis reveled the min effect of vriety nd interction effect of vriety zinc iron hd no significnt effect on strw yield of oth the whet vrieties. An increse in zinc nd iron levels incresed the men grin yield of oth the whet vrieties. Regrding strw yield, oth the vrieties responded well under folir ppliction of zinc nd iron ut UP262, zinc inefficient vriety produced more strw yield s compred to UP2628, zinc efficient vriety. A etter response regrding grin yield of whet ws reported when zinc nd iron were pplied in comintion s compred to the individul ppliction of ech (Hi, 2009). Similr results hve een lso reported y (Seilsepour, 2007) nd (El-Mjid et l., 2000). Seilsepour (2007) found tht the men grin yield of whet ws found to e enhnced y comined ppliction of zinc nd iron in comprison to their individul use (317 mg kg -1 iron nd 330 mg kg -1 zinc). Zn nd Fe content in grins: Zinc levels significntly ffect the grin Zn content of oth the vrieties (Fig 1). The men Zn content of oth the vrieties incresed with incresing Zn levels. The folir spry of 0.25% nd 0.5% zinc sulphte solution incresed the men Zn content y 2.7 nd 10.5 percent s compred to 0 level of zinc sulphte. The ppliction of 0.5% nd 1.0% ferrous sulphte solution decresed the men Zn content y 5.5 nd 7.9 percent, respectively in comprison 452 to no ppliction of ferrous sulphte. The min effect of Fe, vriety nd the interction effect (vriety Zn Fe) were found sttisticlly non significnt regrding their influence on grin Zn content. Overll, the mximum (44.1 µg g -1 ) Zn content ws recorded t 0% Fe+0.5% Zn in UP2628 wheres the minimum (35.0 µg g -1 ) ws lso recorded in vriety UP2628 t 0% Zn+1.0% Fe. Zinc nd Fe levels significntly ffect the Fe content in grins of oth contrsting whet genotypes (Fig 2). Both the vrieties were significntly different from ech other with respect to Fe content in their grins. The men Fe content of oth the vrieties incresed with incresing Fe levels. The folir ppliction of 0.5% nd 1.0% ferrous sulphte solution incresed the Fe content y 14.7 nd 29.6 % s compred to without ppliction of ferrous sulphte, respectively. The interction effect of vriety Zn Fe influenced Fe content in grins significntly. The folir ppliction of 0.25% nd 0.5% zinc sulphte solutions t 0% ferrous sulphte decresed the Fe content y 0.6% nd 11.6% in UP2628 nd y 9.9% nd 23.8% in UP262, respectively. Similrly, t 0.5% ferrous sulphte, the folir spry of 0.25% nd 0.5% zinc sulphte solution reduced the Fe content in UP2628 y 8.8 nd 23.1% while in UP262 the percent reduction ws 3.3 nd 20.3%, respectively. At 1.0% ferrous sulphte level, reduction of 6.8 nd 19.4% ws recorded in UP2628 y the folir ppliction of 0.25% nd 0.5% zinc sulphte solution respectively, wheres in UP262 the reduction of 0.2 nd 2.5% ws found. Overll, the highest Fe content ws recorded in oth the vrieties t comintion of 0% Zn+1.0% Fe while the minimum ws recorded under the comintion of 0% Zn+0.5% Fe for oth the vrieties. The dt on the Zn content depicted tht grin Zn con-
tent of oth the vrieties incresed with incresing (0% to 0.5%) Zn levels nd whtever the reduction in Zn content ws recorded most proly due to incresed (0% to 1.0%) levels of Fe in oth the vrieties. Similrly, incresing Zn levels decresed the Fe content in grins of oth the whet vrieties. Antgonistic interction of Zn nd Fe ws reveled y three mechnisms like competition etween Zn nd Fe with ech other for the ctive site, interference in the process of cheltion during Fe uptke nd trnsloction nd competition etween oth the micronutrient during xylem unloding (Ai-Qing et l., 2011). We found the support of (Ghsemi-Fsei nd Ronghi, 2008), who reveled tht soil ppliction of 8 mg Fe kg -1 soil decresed the men uptke of Zn y 31.9 % in two whet genotypes in comprison to its zero ppliction. It ws lso reported tht high concentrtion of Fe in the medium hmpered vilility nd uptke of Zn (Ds, 2014). A reduction in Fe concentrtion in different plnt prts under incresing regimes of Zn ws lso reported y (Chilin et l., 2015). Conclusion An unmiguous ntgonism etween Zn nd Fe distriution ws oserved in oth contrsting whet genotypes ut UP2628, Zn efficient vriety, exhiited etter uptke nd trnsloction efficiency, thus ccumulted higher Zn nd Fe s compred to UP262. Irrespective of vrietl effect, tretments 0.5% Zn+0% Fe nd 0% Zn+1.0% Fe cn e used for etter vilility of Zn & Fe respectively. More Zn efficient vrieties cn e identified sed on their etter uptke nd trnsloction efficiencies nd cn lso e incorported in crop improvement progrmmes to solve the worldwide prolem of micronutrient deficiency. ACKNOWLEDGEMENTS We re grteful to ICAR, New Delhi, Indi for providing finncil ssistnce during the period of this study. Authors declre tht they hve no conflict of interest. REFERENCES Bhupendr Mthpl et l. / J. Appl. & Nt. Sci. 10 (1): 448-453 (2018) Ai-Qing, Z., Qiong-Li, B., Xio-Hong, T., Xin-Chun, L. nd Gle, W.J. (2011). Comined effect of iron nd zinc on micronutrient levels in whet (Triticum estivum L.). J. Env. Biol. 32: 235-239. Allowy, B.J. (2008). Micronutrients nd crop production. In micronutrient deficiencies in glol crop production. pp 1-39. Chndel, G., Bnerjee, S., See, S., Men, R., Shrm, D.J. nd Verulkr, S.B. (2010). Effects of different nitrogen fertilizer levels nd ntive soil properties on rice grin iron, zinc nd protein contents. Rice Sci. 17: 213-227. Chilin, A., Bncut, R.O., Bncut, I., Setnescu, R., Ion, R.M., Rdulescu, C., Setnescu, T., Stihi, C., Gheoinu, A.I. nd Chelrescu, E.D. (2015). Study of the influence of zinc concentrtion on the sorption nd trnsport of iron in mize y AAS nd EDXRF nlysis techniques. Rom. Reports Phy. 67(3): 1138-1151. 453 Ds, S.K. (2014). Role of micronutrient in rice cultivtion nd mngement strtegy in orgnic griculture-a repprisl. Agric. Sci. 5: 765-769. Elgl, A.M., Ismil, A.S. nd Ossmn, M.A. (2008). Criticl levels of iron, mngnese nd zinc in Egyptin soils. J. Plnt Nut. 9: 267-280. El-Mjid, A.A., Knny, R.E. nd El-Fotoh, H.G.A. (2000). Effect of folir ppliction of some micronutrients on whet yield nd qulity. Ann. Agric. Sci. 1: 301-313. Ghndilyn, A., Vreugdenhil, D. nd Arts, M.G.M. (2006). Progress in genetic understnding of plnt iron nd zinc nutrition. Physiol. Plnt. 126: 407-417. Ghsemi-Fsei, R. nd Ronghi, A. (2008). Interction of iron with copper, zinc nd mngnese in whet s ffected y iron nd mngnese in clcreous soil. J. Plnt Nut. 31(5): 839-848. Hi, M. (2009). Effect of folir ppliction of Zn nd Fe on whet yield nd qulity. Afric. J. Biotech. 8 (24): 6795-6798. Hmlin, R.L. nd Brker, A.V. (2008). Nutritionl llevition of Zn-induced iron deficiency in Indin mustrd nd the effects on zinc phytoremedition. J. Plnt Nut. 31: 2196-2213. Heenn, D.P. nd Cmpell, L.C. (1983). Mngnese nd iron interctions on their uptke nd distriution in soyen (Glycine mx L.). Plnt Soil. 70: 317-326. Imtiz, M., Allowy, B.J., Shh, K.H., Siddiqui, S.H., Memon, M.Y., Aslm, M. nd Khn, P. (2003). Zinc nutrition of whet: : Interction of zinc with other trce elements. Asin J. Plnt Sci. 2: 156-160. Izguirre-Myorl, M.L. nd Sinclir, T.R. (2005). Soyen genotypic difference in growth, nutrient ccumultion nd ultrstructure in response to mngnese nd iron supply in solution culture. Annls Bot. 96: 149-158. Kumr, R., Mehrotr, N.K., Nutiyl, B.D., Kumr, P. nd Singh, P.K. (2009). Effect of copper on growth, yield nd concentrtion of Fe, Mn, Zn nd Cu in whet plnts (Triticum estivum L.). J. Env. Biol. 30: 485-488. Mthpl, B., Srivstv, P.C., Shnkhdhr, D. nd Shnkhdhr, S.C. (2015). Zinc enrichment in whet genotypes under vrious methods of zinc ppliction. Plnt Soil Env. 61(4): 171-175. Pirzdeh, M., Afyuni, M., Khoshgoftrmnesh, A. nd Schulin, R. (2010). Micronutrient sttus of clcreous pddy soils nd rice products: Impliction for humn helth. Biol. Fert. Soil. 46: 317-322. Prksh, I.G., Bu, N.M., Rmchndr, P. nd Mllikrjun, B.R. (2016). Breeding crop plnts for improved humn nutrition through iofortifiction: Progress nd prospects. J.M. Al-Khyri et l. (eds). Advnces in Plnt Breeding Strtegies: Agronomic, Aiotic nd Biotic Stress Trits. DOI 10.1007/978-3-319-22518-0-2. Seilsepour, M. (2007). The study of Fe nd Zn effects on quntittive nd qulittive prmeters of winter whet nd determintion of criticl levels of these elements in Vrmin plin soils. Pjouhesh Szndegi. 76: 123-133. Shhzd, Z., Rouched, H. nd Rkh, A. (2014). Comting minerl mlnutrition through iron nd zinc iofortifiction of cerels. Com. Rev. Food Sci. Food Sf. 13:329-346 Singh, B., Ntesn, S.K.A., Singh, B.K. nd Ush, K. (2005). Improving zinc efficiency of cerels under zinc deficiency. Curr. Sci. 88 (1): 36-44. Slimn, Z.T. (1990). Effect of Zn on Fe-stress-response mechnism of two soyen genotypes. Agric. Sci., 2:61-69 Welch, R.M. nd Grhm, R.D. (2004). Breeding for micronutrients in stple food crops from humn nutrition perspective. J. Exp. Bot. 55: 353-364.