Raw Material Management From Slaughtering Plant to Rendering

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Raw Material Management From Slaughtering Plant to Rendering 2018 Jim Mann KEMIN IS ASSURANCE

Kemin s Involvement in Pet Food Supply Chain Slaughter Houses Renderers Field Fresh/Frozen Meat Collectors Crops Pet Food Manufacturers The pet food manufacturing process creates multiple opportunities to influence taste

Pet Food Quality Nutrient Profile Ingredient Legend Design Ingredient Quality Palatability Targets Formulation and Design Oxidation Control Moisture Control Process Control and Consistency Food Safety Programs Process Target Shelf Life Moisture Migration Packaging Distribution External Factors Kemin Industries, Inc. and its group of companies 2018 all rights reserved. Trademarks of Kemin Industries, Inc., U.S.A.

Raw Material Stabilization Managing Customer expectations Efficacious alternatives to address consumer concerns Improving ingredient quality expect fresh, stable ingredients Bring new insights and innovations to industry Support safety and regulatory needs Managing Consumer expectations Expect fresh and stable petfood Concern about safety of synthetic chemical additives Humanization of pets Concern over animal well-being

Impact of Raw Material Degradation Oxidative Deterioration Shelf-life reduction Microbial Degradation Free Fatty Acids (FFA), Biogenic Amines (BA) Impact on Plant Operations FFA increases energy costs Longer cook times due to decreased heat transfer Reduced efficiency Increased fines Reduced fat yield Increased plant odor Reduction in nutritive value 1 1) Batch dry rendering: The influence of controlled processing conditions on the quality of meat meal prepared from sheep stomachs, Bremner, A.H., April 1976, Journal of Science of Food and Agriculture, pp 307-314

Raw Material Management Many factors influence the quality of rendered products Composition of offal DAF, bone residue, blood Freshness/Age of Offal (FFA, Biogenic Amines) Usage of sanitizing agents unknown effect Influence both oxidative and microbial quality

Role of Antioxidants Antioxidants arrest rancidity by sacrificing themselves rather than allowing the oxidation of the fat or food nutrients Metallic Ions Chelator AOX Breaking Chain Reaction O 2 Stable Free Radicals Oxygen Scavenger Krabbe, E. Quality of raw materials AMVEA Peru, 2013

Relative Reaction Rate Moisture Water Activity Affects Biological Activities Lipid Oxidation Moisture Sorption Isotherm 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0 Water Activity Increasing level of Microbial Risk Lubuza, T. P. 1971. Kinetics of Lipid Oxidation in Foods, CRC Crit. Rev. Food Technol. 2: 355-405.

Free Fatty Acids Origins Origins: Triglycerides hydrolyzed by lipase enzymes coming from Bacterial contamination 1 Temperature increases the lipases activity until 60 c @ph=9 2 Sources: (1)FEMS Microbiol Rev. 1994 Sep; 15(1):29-63. Bacterial lipases. Aeger KE, Ransac S, Dijkstra BW, Colson C, Van Heuvel M, Misset O (2) Ghori MI, Iqbal MJ, Hameed A - Braz. J. Microbiol. (2011).Characterization of a novel lipase from Bacillus sp. isolated from tannery wastes, pp 22-29.

Free Fatty Acid Control Poultry fat Allinsur FS 6 food grade liquid solution applied @7,5kg/t in Viscera just after evisceration. KNE #9145

Free Fatty Acid Control Poultry Meal Poultry Meal Allinsur FS 6 food grade liquid solution applied @7,5kg/t in Viscera just after evisceration KNE #9145

Free Fatty Acid Control 6.0 5.0 4.0 3.0 2.0 1.0 0.0 FFA (Average) % in Poultry Fat 2.9 @4kgs Allinsur FS11 /t of viscera 2.7 @5kgs Allinsur FS11 /t of viscera 4.1 No treatment 10.0 9.0 8.0 7.0 6.0 5.0 4.0 3.0 2.0 1.0 0.0 Poultry Meal FFA (Average) % in Poultry Meal 5.0 4.8 @4kgs Allinsur FS11 /t of viscera @5kgs Allinsur FS11 /t of viscera 6.9 No treatment KN 160007855-7820 -7926

Free Fatty Acid Control Free Fatty Acid Levels in Poultry Fat 4 kg/mt Allinsur FS-11 29% Reduction in FFA 5 kg/mt Allinsur FS-11 34% Reduction in FFA 7.5 kg/mt Allinsur FS-6 46% Reduction in FFA **Allinsur treatment added to viscera prior to rendering CLS KN 160007855, 7820, 7926 CLS KNE 9145

Amino Acid Precursors and Parent Amino Acid Histidine Lysine Tyrosine Tryptophan Serine Methionine Arginine Phenylalanine Aspartic acid Glutamic acid Threonine Cysteine Ornithine Biogenic Amine Histamine Cadaverine Tyramine Tryptamine Ethanolamine Spermidine/spermine Agmatine/putrescine Phenethylamine Beta-alanine Gamma-amino butyric acid 2-hydroxypopylamine Beta-mercaptoethylamine Putrescine/spermidine Biogenic Amines Can be catalyzed by endogenous or bacterial amino acid decarboxylases Cadaverine, Putrescine, and Tyramine are primary biogenic amines (Histamine in fish) Categorized as either vasoactive or psychoactive Vasoactive amines include tyramine Psychoactive amines include histamine, putrescine, & cadaverine

Biogenic Amines Ruiz Capillas. 2004

Biogenic Amines: Important Roles IgE + mastcell Allergic Reaction mastcell degranulation indirect vegetative release mediators (e.g., histamine) direct nervous system indirect lungs skin intestine indirect direct Non allergic Reaction Food intolerance biogenic amines Adapted from B. ten Brink et al., Occurrence and formation of biologically active amines in foods, Int. J. Food Microbiol. 11 (1990) 73-74 e.g., histamine

Biogenic Amines in Chilled (4º C) Chicken Viscera vs. Storage Days and TPC Biogenic Amine Washed Viscera (ppm) Unwashed Viscera (ppm) Day 0 Day 3 Day 0 Day 5 Tyramine 6 148 3 537 Putrescine 2 6 4 203 Cadaverine 1 96 18 280 Histamine 6 12 3 32 Spermidine 42 47 49 139 Subtotal 1 57 309 77 1191 Agmatine 13 9 17 2 Spermine 20 18 33 27 Subtotal 2 + 1 34 28 51 30 Total BA 90 336 127 1220 ph 6.2 5.8 5.8 5.3 TPC (cfu/g) 6 X 10 4 2 X 10 7 2 X 10 6 6 X 10 8 CLS Project #06-0118

Histamine Control in Poultry Viscera SD-16-00078

Biogenic Amine Control in Poultry Viscera SD-16-00078

Biogenic Amine Control in Poultry Meal 600 Poultry Meal Biogenic Amines ppm 541 500 400 378 300 200 100 0 158 107 Putrescine ppm 1 259 154 Cadaverine ppm 42 24 89 Histamine ppm 109 108 109 Spermine ppm 216 208 205 Spermidine ppm 4 kgs Allinsur SF11 /T of viscera 5 kgs Allinsur SF11 /T of viscera Untreated viscera Maxi limit Histamine ppm KN 160007855-7820 -7926

Biogenic Amines in Various Meals Mean concentration of putrescine, cadaverine, and histamine in Australian by-product meals between 1994-1997

Odor Issues in Rendering Plants Gasses generated in rendering plant include, H 2 S, SO 2 and NH 3, among others Maximum allowable concentrations for workspace have been established for these gasses Anecdotal evidence has demonstrated that controlling microbial degredation of rendering raw materials can have positive effect on reduction of plant odors

Allinsur FS: Possible Applications Viscera treatments Fresh meat Slurries, Digest Protein Hydrolysates Bone treatment Feathers

Allinsur FS application in Slaughterhouse

Allinsur FS Protecting the Quality of Rendering Raw Materials The value of controlling oxidation and microbial growth is well recognized and documented Poor ingredient quality (both microbial and oxidative) has been linked to quality issues in finished pet food products Reduction in palatability observed in both cat and dog trials Decrease in finished product shelf-life Reduction of FFA in fats and protein meals could increase value and marketability of rendered products Potential impact on pathogen control Maintaining quality of ingredients may reduce energy costs Improve shelf-life and palatability of rendered ingredients and petfood products

The Raw Material Challenge Biogenic amines produced from spoiled foodstuffs can negatively impact important physiological functions, depending upon levels (Histamine, Tyramine, Cadaverine, Putrecine) Daily long-term feeding effects of biogenic amines on reproducing females, growing puppies and kittens or ill pets unknown Unknown impact of biogenic amines on animal health and allergy risk Potential to decrease odors originating from slaughterhouse and rendering plant Potential to provide energy savings in the rendering process Decayed raw materials are more difficult to process Reduction of heat transfer ability of fat requiring longer cooking times Increased fines decreases efficiency of presses and decreases fat yields Treat materials at slaughterhouse to maximize benefit Dosage dependant on many factors Typically 3-5 kg / MT

Kemin Has Expertise Along Entire Supply Chain Food Safety Antioxidants Health & Nutrition Palatants SLAUGHTERHOUSES FRESH/FROZEN MEAT COLLECTION RENDERING PET FOOD INDUSTRY DOGS & CATS Food Safety Antioxidants Kemin Industries, Inc. and its group of companies 2018 all rights reserved. Trademarks of Kemin Industries, Inc., U.S.A.

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