TEST Directions: Circle the letter indicating whether the following statements are either true ("T") or false ("F"). T F 1. A closed glass jar of pickles is a closed system. T F 2. Cellular respiration only occurs in animal cells. T F 3. Diffusion is a process which results as a result of unequal concentration of particles in a given space. T F 4. All cells are surrounded by a protective cell wall. T F 5. Water is released from plant cells through the process of transpiration. T F 6. Plant cells exchange matter and energy through their cell membrane. T F 7. The process of water and minerals moving into a plant cell is call active transport. T F 8. Diffusion and osmosis depend upon a concentration gradient. T F 9. Cellular respiration and combustion reactions have no common elements. T F 10. Cell membranes are described as being permeable because they allow unrestricted movement of matter in and out of the cell.
GLOSSARY Active transport movement of matter into, or out of, a cell which requires energy from the cell; usually this involves movement against the concentration gradient Adenosine triphosphate (ATP) the molecule used in cell processes as a supply of energy; it is produced by cells during cellular respiration Alveoli microscopic air sacs in the lungs; the site where most gas exchange occurs Capillaries microscopic blood vessels; most matter exchange between body cells and the blood occurs through the walls of the capillaries Carrier protein a molecule in the cell membrane that allows materials to pass through it Cell membrane the outer semipermeable covering of the cell; it is very thin and flexible Cellular respiration the process that converts the potential energy of food into other useful forms; its most important product is ATP Concentration gradient a difference in concentration of a particle at two points in a space Diffusion net movement of particles from an area of high concentration to an area of low concentration Endocytosis a process cells use to capture large particles; the cell membrane wraps around the particle and encloses it Epidermis an outer layer of cells covering plants and animals; its primary function is protection Exocytosis a process cells use to expel material; a vacuole connects to the cell membrane and opens so its content is now outside of the cell Guard cells the specialized plant cells which surround, and control the opening and closing of, the stoma Lenticels small pores visible on the stems of many plants; important for gas exchange Metabolic rate the rate at which an organism is converting energy Osmosis diffusion of water through a membrane Passive transport diffusion of materials across a membrane requiring no energy input Root hairs microscopic extensions from the surface cells of roots Semipermeable membrane a pliable tissue which allows some materials to pass, but stops others; cell membranes are semipermeable Stomata (singular: stoma) small openings on the surface of plant leaves which can open and close to control the movement of gases, particularly water vapor Surface area to volume ratio a value calculated by dividing the surface area of an object by the volume it contains; this ratio is large for cells efficient at exchanging materials with their surroundings
Write the balanced chemical equation for cellular respiration. C6H12O6(s) + 6O2(g) 6CO2(g) + 6H2O(l) + energy CELLULAR RESPIRATION Material constantly enters and leaves plant cells. Water, oxygen, carbon dioxide and nutrients pass through the cell membrane easily. Because some materials are able to pass through and some are stopped, the cell membrane is semipermeable. 1. Compare a single-celled organism, Amoeba, with a multicellular organism, a fish. Explain what special problems each must overcome to get the nutrients it requires.
GAS EXCHANGE IN PLANTS Plant leaves have a thick outer layer to protect them from the drying air. To allow gas exchange, there are small holes on the surface of the leaf. These holes are called stomata. They open when two beanshaped cells (known as guard cells) around the stoma fill with water. guard cells closed open stoma The stem of a plant has openings called lenticels. You can often see them on new shoots in the spring, or at the base of spruce trees. Roots also exchange gases. Finger-like projections called root hairs extend from the surface of the root. These increase the surface area enormously and speed up the exchange. The root hairs absorb gases dissolved in water in the soil. Plant Root Systems leaves stem root root tip root hairs 2. The epidermis of a plant leaf is tough and waxy and makes gas exchange more difficult. What benefit does an epidermis have for the plant? 3. Most plants have many more stomata than lenticels. Why? 4. Why must roots be wet to function properly? 5. Would you expect carbon dioxide to diffuse into or out of stomata during the daylight hours? What about oxygen?
GAS EXCHANGE IN ANIMALS All of our cells require oxygen, which we absorb in our lungs. Predict how the oxygen gets from the lungs to all the cells of the body. Gases and other nutrients are carried in the blood to all parts of the body. Large organisms, like human beings, need to absorb gases. We also require a method of moving the absorbed gas around the body. Lungs are structured to allow efficient gas exchanges. Lungs are not empty sacks, but tissues that are finely divided. This increases the surface area of the lung, allowing for speedy gas exchange. Once absorbed, gases must be transported to all parts of the body. For that reason the alveoli are surrounded by blood-carrying capillaries. A fish s gills act in the same way. They are red because they are filled with blood-carrying capillaries. Gases exchange between the water and the blood by diffusing through the membranes of the gills. Gill Filament water flow capillaries lamella oxygenated blood deoxygenated blood 6. Do fish and humans exchange the same gases with their environments? 7. Why would gills not function properly for land animals? 8. Why are gills and lungs both surrounded by capillaries? 9. Investigate the gas exchange system of a grasshopper.
PASSIVE TRANSPORT 10. Compare and contrast diffusion and osmosis. 11. Food can be preserved by salting it. What would happen to the water inside bacteria when they are in an extremely salty environment? 12. The contractile vacuole of a Paramecium constantly pumps water out of the cell. Why would a Paramecium need to remove water? 13. After a long bath your finger tips look all wrinkled. Why? 14. Reverse osmosis is a method of purifying water. Considering the meaning of osmosis, explain how reverse osmosis would make water more pure. 15. Some people claim to learn by osmosis does this make sense? Pour a teaspoon full of vinegar onto a paper towel or cloth and put it into a plastic bag. Close the bag and wait about five minutes. Hold the bag at arm s length in a room without any breeze or draft. Slowly remove the cloth from the bag. Count how many breaths you can take before you smell the vinegar. This gives you a rough idea of the speed of diffusion of vinegar molecules through air. Inside and outside cells, water will diffuse from higher to lower concentration until it reaches equilib-
ACTIVE TRANSPORT The living yeast cells use active transport to remove dye molecules. Because of this, they do not become coloured. Once killed by boiling, the cells are no longer able to do active transport and will pick up the colour of the dye. Cells use endocytosis and ectocytosis to acquire and remove large particles which cannot be accommodated by diffusion. Endocytosis Ectocytosis 16. Explain the difference between endocytosis and exocytosis. 17. What two organelles are involved in endocytosis? 18. What is the role of adenosine triphosphate (ATP) in active transport? 19. A Paramecium s contractile vacuole pumps water out of the cell. Is this active or passive transport? 20. Describe the role of carrier proteins in active transport. If a microscope is available, mix household yeast with water and a drop of food colouring. Prepare a wet mount slide and observe the yeast cells. Now boil the yeast and water (a microwave oven will work). Observe the yeast under the microscope once again. Compare the yeast cells before and after boiling.
CELL SIZE Place your hand, with your fingers together, flat on a blank page and trace it with a pencil. Measure the length of the line you drew. (Use a piece of string!) Now do the same thing again, except spread out your fingers. Compare the length of the two lines. Describe how this activity demonstrates the ideas of surface area and volume. The length of the line corresponds to the surface area. The space your hand occupies corresponds to the volume. By spreading your fingers you increased the surface area, but the volume of your hand remained unchanged. This shows that dividing objects into smaller pieces increases the surface area to volume ratio. Calculating surface area to volume ratio of a cube. 1. Find the surface area to volume ratio of a sugar cube with sides 1.0 cm in length. surface area volume = = = number of sides area of one side volume n(l w) l w h 6(1.0 cm)(1.0 cm) (1.0 cm)(1.0 cm)(1.0 cm) = 6.0 cm2 1.0 cm3 = 6.0* * Note that surface area to volume ratios are reported without units. 21. Find the surface area to volume ratio for a cube with sides 0.20 cm long. 22. Does the surface area to volume ratio increase or decrease as a cell gets larger? 23. What is more efficient for exchange of materials with the surroundings, a large or a small surface area to volume ratio? 24. Sketch a scale diagram of two cells with a volume of 12 cm3: one with dimensions of 3 cm x 2 cm x 2 cm, the second with dimensions 12 cm x 1 cm x 1 cm. 25. Which of the two cells you sketched has the greater surface area to volume ratio? Which cell would be more efficient at exchanging materials with the environment?
POST-TEST MULTIPLE CHOICE Directions: Decide which of the choices best completes the statement or answers the question, then circle the letter that corresponds to your choice. (3 marks each) 1. The guard cells control the opening and closing of a. contractile vacuoles b. oral grooves c. lenticels d. stomata 2. For cells with a volume of 16 cm3, the shape with the most efficient surface-to-volume ratio is a. 1 cm x 1 cm x 16 cm b. 1 cm x 2 cm x 8 cm c. 1 cm x 4 cm x 4 cm d. 2 cm x 2 cm x 4 cm 3. Carrier or transport proteins facilitate a. osmosis b. diffusion c. active transport d. passive transport 4. Cells absorb large particles or molecules which are essential for life functions, but cannot pass through the cell membrane directly, by the process of a. exocytosis b. endocytosis c. active transport d. passive transport 5. Plant roots absorb minerals by means of a. osmosis b. diffusion c. active transport d. passive transport
POST-TEST LONG ANSWER Directions: Answer the following questions in the spaces provided. Use the back of the sheet if 1. Cell membranes are considered semipermeable because.(4 marks) 2. is the process which provides cells with the energy required for life functions. (3 marks) 3. Plants have openings in the stem called for the purpose of gas exchange with the environment. (3 marks) 4. The specialized root cells which facilitate the exchange of gases, water and nutrients are the. (3 marks) 5. Oxygen in the air is exchanged for carbon dioxide in the blood in the of our lungs. (3 marks) 6. The movement of materials across a cell membrane which requires no energy input from the cell is called. (3 marks) 7. Explain why a red blood cell begins to swell shortly after being placed into a saline solution. (10 marks) 8. Osmosis is. (3 marks) 9. The acronym ATP stands for which provides the energy for active transport. (3 marks) 10. A student, examining to types of cells, estimates the surface-to-volume ratio for each. Cell "A" has a ratio of 2500:1 and cell "B" a ratio of 750:1. Cell is more efficient than cell in carrying out its life functions. (6 marks)
POST-TEST 11. List three examples of anatomical, or technological, structures which take advantage of the surface-to-volume ratio principle. (9 marks) 12. The second law of thermodynamics states that heat flows from matter at a higher temperature to matter at a lower temperature. How does this compare to the process of diffusion. (9 marks) 13. A Paramecium uses a to remove water from the cell. This is an example of transport. (6 marks) 14. Why is moisture in the soil essential for proper root function? (8 marks) 15. A plant cell is placed into a salt solution. Water molecules will most likely move through the membrane into by. (6 marks) 16. In animals with a closed circulatory system the capillaries are the site of between the blood and. (6 marks)