Reporter System Luciferase Reporter Stable Cell Lines One-Step Luc Assay Kit Stable Cell Lines www.abeomics.com
Reporter Cell Lines Reporter cell lines are used for cell-based assays for screening of drugs/compounds under the stimulatory or inhibitory effects. Abeomics have developed several engineered stable cell lines, where luciferase is used as a reporter gene, which is under the control of upstream promoters. These stable cell lines can be used to study stimulation or inhibitions of receptors, kinases and transcriptional factors. STAT Leeporter TM Luciferase Reporter Cell Lines STAT Leeporter TM Luciferase Reporter Cell Lines as a tool for cancer therapeutic target screening: Signal transducer and activator of transcription (STAT) proteins are closely related with human cancers. STAT1 STAT1 Leeporter TM Luciferase Reporter HeLa Cell Line (14-112ACL) Relative STAT1- Luciferase Activity 1 1 hifnγ (ng/ml) STAT1 Leeporter TM (HeLa) hifnγ, EC 5 = 2.6 ng/ml -2-1 1 2 3 4 log[hifnγ], (ng/ml) STAT1 Leeporter TM Luciferase Reporter RAW264.7 Cell Line (14-113ACL) Relative STAT1-Luciferase Activity 9 7 5 3 1 STAT1 Leeporter TM (RAW264.7) mifnγ, EC 5 = 4.73 ng/ml mifnγ (ng/ml) 1 2 3 4 log[mifnγ], (ng/ml) STAT3 STAT3 Leeporter TM Luciferase Reporter HEK293 Cell Line (14-114ACL) Relative STAT3-Luciferase Activity 25 15 1 5 IL-6 (ng/ml) Relative STAT3-Luciferase Activity 16 14 12 1 8 6 4 2 IL-6 - + + + + + + + + + IL-6Ra Ab (ng/ml) 4.6 14 41 123 37 111 333 - STAT3 Leeporter TM (HEK293) IL-6 [EC 5 =8.3ng/ml] Anti-IL-6Ra [IC 5 =.21ug/ml] -3-2 -1 1 2 3 log[anti-il-6r α], (ug/ml) log[anti-il-6], (ng/ml) age 2
Reporter Cell Lines STAT4 STAT4 Leeporter TM Luciferase Reporter HEK293 Cell Line (14-1ACL) Relative STAT4-Luciferase Activity 3 25 15 1 5 IFNγ (ng/ml) STAT4 Leeporter TM (HEK293) IFNγ, EC 5 = 1.82 ng/ml -2-1 1 2 3 4 log[ifnγ], (ng/ml) STAT5 STAT5 Leeporter TM Luciferase Reporter Ba/F3 Cell Line (14-135ACL) Relative STAT5-Luciferase Activity 5 45 35 3 25 15 1 5 mil-3 (ng/ml) 1 STAT5 Leeporter TM (Ba/F3) IL-3, EC 5 =.6 ng/ml -2-1 1 2 3 log[il-3], (ng/ml) STAT Reporter Cell Line list: roduct Name Cat.No. Host Luciferase STAT1 Leeporter Luciferase Reporter Cell Line 14-112ACL Hela Renilla STAT1 Leeporter Luciferase Reporter Cell Line 14-113ACL RAW 264.7 Renilla STAT3 Leeporter Luciferase Reporter Cell Line 14-114ACL HEK 293 Renilla STAT4 Leeporter Luciferase Reporter Cell Line 14-1ACL HEK 293 Renilla STAT5 Leeporter Luciferase Reporter Cell Line 14-135ACL Ba/F3 Renilla age 3
Reporter Cell Lines TLR Leeporter TM Luciferase Reporter Cell Lines as a tool for studying TLR signaling pathways and screening agonists or antagonists. TLR2/NF-kB LeeporterTM (HEK293) am3csk4, EC 5 = 2.11 ng/ml - -2-1 1 2 3 log[am3csk4], (ng/ml) TLR3/NF-kB LeeporterTM (HEK293) oly(i:c), EC 5 = 64.5 ug/ml - -1 1 2 3 4 log[oly(i:c)], (ug/ml) 11 TLR3/IFNB LeeporterTM (HEK293) oly (I:C), EC 5 = 88.9 ug/ml 9 7 5 3 1-1 -1 1 2 3 4 log[poly(i:c)], (ug/ml) 11 TLR3/ISRE LeeporterTM (HEK293) oly (I:C), EC 5 = 66.4 ug/ml 9 7 5 3 1-1 -1 1 2 3 4 log[poly(i:c)], (ug/ml) 11 TLR4/IL-8 LeeporterTM (HeLa) LS, EC 5 = 5.4 ng/ml 9 7 5 3 1-1 -2-1 1 2 3 log[ls], (ng/ml) TLR5/NF-kB LeeporterTM (HEK293) Flagellin, EC 5 = 6.52 ng/ml - -3-2 -1 1 2 3 log[flagellin], (ng/ml) TLR7/NF-kB LeeporterTM (HEK293) R848, EC 5 = 1.6 ug/ml - -2-1 1 2 log[r848], (ug/ml) TLR8/NF-kB LeeporterTM (HEK293) R848, EC 5 = 26.43 ug/ml - -1 1 2 3 log[r848], (ug/ml) TLR9/NF-kB LeeporterTM (HEK293) CpG, EC 5 = 5.52 ug/ml - -1 1 2 log[cpg], (ug/ml) TLR Reporter Cell Line list: roduct Name Cat.No. Host Luciferase TLR2/NF-kB Leeporter TM Luciferase Reporter Cell Line 14-127ACL HEK 293 Renilla TLR3/NF-kB Leeporter TM Luciferase Reporter Cell Line 14-128ACL HEK 293 Renilla TLR3/IFNB Leeporter TM Luciferase Reporter Cell Line 14-122ACL HEK 293 Renilla TLR3/ISRE Leeporter TM Luciferase Reporter Cell Line 14-123ACL HEK 293 Renilla TLR4/IL-8 Leeporter TM Luciferase Reporter Cell Line 14-124ACL HeLa Renilla TLR5/NF-kB Leeporter TM Luciferase Reporter Cell Line 14-139ACL HEK 293 Renilla TLR7/NF-kB Leeporter TM Luciferase Reporter Cell Line 14-129ACL HEK 293 Renilla TLR8/NF-kB Leeporter TM Luciferase Reporter Cell Line 14-13ACL HEK 293 Renilla TLR9/NF-kB Leeporter TM Luciferase Reporter Cell Line 14-131ACL HEK 293 Renilla age 4
Reporter Cell Lines TCF/LEF (Wnt) Leeporter TM Luciferase Reporter Cell Line as a tool for Wnt signaling pathway research: Analysis of the LiCl-mediated TCF/LEF induction activity. Relative TCF/LEF-Luciferase Activity LiCl (mm) TCF/LEF LeeporterTM (HEK293) LiCl, EC 5 = 1.2 mm -.5.75 1. 1.25 1.5 1.75 log[licl], (mm) Analysis of the Wnt3a-mediated TCF/LEF induction and inhibitory activity of IWR-1 (Inhibitor of Wnt Response 1). Relative TCF/LEF-Luciferase Activity 12 1 8 6 4 2 Human Wnt3a (ng/ml) Relative TCF/LEF-Luciferase Activity 14 12 1 8 6 4 2 LiCl + + + + + + + + + + + Wnt3a - + + + + + + + + + + IWR-1 (um).5.1.5.25 1.25 6.25 31.25 62.5 125 - TCF/LEF Leeporter TM (HEK293) Wnt3a [EC 5 =54.2ng/ml] IWR-1 [IC 5 =.168uM] -3-2 -1 1 2 3 4 log[iwr-1], (um) log[wnt3a], (ng/ml) roduct Name Cat.No. Host Luciferase TCF/LEF Leeporter Luciferase Reporter Cell Line 14-119ACL HEK 293 Renilla age 5
Complete List of Reporter Cell Lines roduct Name Cat.No. Host Luciferase A-1 Leeporter Luciferase Reporter Cell Line 14-12ACL HEK 293 Renilla ATF6 Leeporter Luciferase Reporter Cell Line 14-138ACL HeLa Renilla CRE Leeporter Luciferase Reporter Cell Line 14-134ACL HEK293 Renilla FOX3 Leeporter Luciferase Reporter Cell Line 14-144ACL Jurkat T Renilla GATA3 Leeporter Luciferase Reporter Cell Line 14-19ACL HEK 293 Renilla HRE Leeporter Luciferase Reporter Cell Line 14-118ACL HeLa Renilla IL-6 Leeporter Luciferase Reporter Cell Line 14-116ACL NIH 3T3 Renilla IL-8 Leeporter Luciferase Reporter Cell Line 14-11ACL RAW 264.7 Renilla inos Leeporter Luciferase Reporter Cell Line 14-115ACL RAW 264.7 Renilla ISRE Leeporter Luciferase Reporter Cell Line 14-141ACL RAW 264.7 Renilla ISRE Leeporter Luciferase Reporter Cell Line 14-143ACL HEK293 Renilla MDA5/NF-kB Leeporter Luciferase Reporter Cell Line 14-121ACL HEK 293T Renilla MI-2 Leeporter Luciferase Reporter Cell Line 14-13ACL HEK 293 Renilla MI-2 Leeporter Luciferase Reporter Cell Line 14-14ACL RAW 264.7 Renilla MRE Leeporter Luciferase Reporter Cell Line 14-137ACL HEK293 Renilla NFAT Leeporter Luciferase Reporter Cell Line 14-11ACL HEK 293 Renilla NFAT Leeporter Luciferase Reporter Cell Line 14-111ACL RAW 264.7 Renilla NF-kB Leeporter Luciferase Reporter Cell Line 14-ACL RAW 264.7 Renilla NF-kB Leeporter Luciferase Reporter Cell Line 14-125ACL HEK 293 Renilla NF-kB Leeporter Luciferase Reporter Cell Line 14-142ACL Jurkat T Renilla Nrf2 Leeporter Luciferase Reporter Cell Line 14-117ACL MCF7 Renilla p53 Leeporter Luciferase Reporter Cell Line 14-126ACL HeLa Renilla RIG-I/NF-kB Leeporter Luciferase Reporter Cell Line 14-ACL HEK 293T Renilla SBE Leeporter Luciferase Reporter Cell Line 14-136ACL HEK293 Renilla SRE Leeporter Luciferase Reporter Cell Line 14-132ACL HEK 293 Renilla SRF-RE Leeporter Luciferase Reporter Cell Line 14-133ACL HEK293 Renilla STAT1 Leeporter Luciferase Reporter Cell Line 14-112ACL HeLa Renilla STAT1 Leeporter Luciferase Reporter Cell Line 14-113ACL RAW 264.7 Renilla STAT3 Leeporter Luciferase Reporter Cell Line 14-114ACL HEK 293 Renilla STAT3 Leeporter Luciferase Reporter-Ba/F3 Cell Line 14-147ACL Ba/F3 Renilla STAT4 Leeporter Luciferase Reporter Cell Line 14-1ACL HEK293 Renilla STAT4 Leeporter Luciferase Reporter-Ba/F3 Cell Line 14-145ACL Ba/F3 Renilla STAT5 Leeporter Luciferase Reporter Cell Line 14-135ACL Ba/F3 Renilla STAT6 Leeporter Luciferase Reporter-Ba/F3 Cell Line 14-146ACL Ba/F3 Renilla TCF/LEF Leeporter Luciferase Reporter Cell Line 14-119ACL HEK 293 Renilla TLR2/NF-kB Leeporter Luciferase Reporter Cell Line 14-127ACL HEK 293 Renilla TLR3/IFNB Leeporter Luciferase Reporter Cell Line 14-122ACL HEK 293 Renilla TLR3/ISRE Leeporter Luciferase Reporter Cell Line 14-123ACL HEK 293 Renilla TLR3/NF-kB Leeporter Luciferase Reporter Cell Line 14-128ACL HEK 293 Renilla TLR4/IL-8 Leeporter Luciferase Reporter Cell Line 14-124ACL HeLa Renilla TLR5/NF-kB Leeporter Luciferase Reporter Cell Line 14-139ACL HEK 293 Renilla TLR7/NF-kB Leeporter Luciferase Reporter Cell Line 14-129ACL HEK 293 Renilla TLR8/NF-kB Leeporter Luciferase Reporter Cell Line 14-13ACL HEK 293 Renilla TLR9/NF-kB Leeporter Luciferase Reporter Cell Line 14-131ACL HEK 293 Renilla TNF-alpha Leeporter Luciferase Reporter Cell Line 14-15ACL HEK 293 Renilla TNF-alpha Leeporter Luciferase Reporter Cell Line 14-16ACL RAW 264.7 Renilla TNF-beta Leeporter Luciferase Reporter Cell Line 14-17ACL HEK 293 Renilla TNF-beta Leeporter Luciferase Reporter Cell Line 14-18ACL RAW 264.7 Renilla age 6
Stable Cell Lines Abeomics offers monoclonal stable cell lines expressing various target molecules including immune-check point proteins. Detection of human D-1 in the CHO-K1/D-1 stable cell line by surface Flow Cytometry. CHO-K1 cells (Green); CHO-K1/D-1 (Red). Detection of Cas9 in the HEK293/Cas9 stable cell line by Western blotting using anti-cas9 antibody. Stable Cell Line list: Cat. No. roduct Name Host Description 14-5ACL D-1 Stable Cell Line CHO-K1 Human rogrammed Cell Death rotein -1 (D-1, also known as CD279). 14-51ACL D-L1 Stable Cell Line CHO-K1 Human rogrammed Death- Ligand 1 (D-L1, also known as CD275 and B7-H1). 14-52ACL D-L2 Stable Cell Line CHO-K1 Human rogrammed Death- Ligand 2 (D-L2, also known as B7-DC and CD273). 14-53ACL ICOS Stable Cell Line CHO-K1 Human Inducible T-Cell Costimulator (ICOS, also known as CD278). 14-54ACL ICOSL Stable Cell Line CHO-K1 Human ICOS Ligand (ICOSL, also known as B7-H2, B7R-1, and CD275). 14-55ACL CD Stable Cell Line CHO-K1 Human Cluster of Differentiation (CD, also known as B7-1). 14-56ACL CTLA4 Stable Cell Line CHO-K1 Human Cytotoxic T-Lymphocyte-Associated rotein 4 (CTLA4, also known as CD152). 14-57ACL hb7-h4 Stable Cell Line CHO-K1 Human B7-H4, also known as VTCN1. 14-58ACL mb7-h4 Stable Cell Line CHO-K1 Mouse B7-H4, also known as VTCN1. 14-59ACL Langerin Stable Cell Line CHO-K1 Human Langerin, also known as CD7. 14-51ACL Cas9 Stable Cell Line HEK293 CRISR associated protein 9 (Cas9, also known as Csn1). 14-511ACL NKp3 Stable Cell Line CHO-K1 Human activating Natural Killer cell receptor 3 (NKp3, also known as Natural Cytotoxicity) triggering Receptor 3 (NCR3) or CD337). 14-512ACL NKp46 Stable Cell Line CHO-K1 Human activating Natural Killer cell receptor 46 (NKp46, also known as Natural Cytotoxicity) triggering Receptor 1 (NCR1) or CD335). age 7
One-Step Luciferase Assay Kit roduct Name Cat. No. Quantity 96-well late Format Leeporter Luciferase Assay Reagent 17-111 1 Kit Tests The Leeporter Luciferase Assay Reagent was specifically formulated to use with our Leeporter (Luciferase Reporter) Cell Line products, which was designed to produce highly sensitive signal and prolonged signal intensity. As one-step glow assay reagent, the Leeporter Luciferase Assay Reagent can be directly added to cell culture plates compatible with the luminometer being used without diluting or transferring culture supernatants or cell lysates to other plates. IL-6 IL-6 JAK gp13 IL-6R gp13 JAK STAT3 STAT3 Luciferase (Ren Luc) STAT3 STAT3 STAT3-RE Luciferase Ren Luc Coelenteramide Nucleus Cytoplasm Light Coelenterazine Contact Us 9853 acific Heights Blvd. Suite D, San Diego, CA 92121 USA Tel: +1-858-263-4982 Fax: +1-858-5-1192 Email: support@abeomics.com www.abeomics.com