Nutrient digestibility in canola meal for broilers: Effects of oil extraction method and fractionation by air classification

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Nutrient digestibility in canola meal for broilers: Effects of oil extraction method and fractionation by air classification Matt Oryschak *1, Doug Korver 2 and Eduardo Beltranena 1,2 1 Alberta Agriculture and Rural Development, Edmonton, AB, Canada 2 University of Alberta, Edmonton, AB, Canada Abstract 211

Background Canada is cold! i.e., we can t grow soybeans in Western Canada Canola is our dominant oilseed Low erucic acid, low glucosinolate version of rapeseed

Key canola industry trends Domestic canola crush 000 s of T $CDN/T 6000 300 Meal Total Crush 4787 5000 250 Canola meal price 4000 3000 2684 200 150 2000 100 1000 50 0 0

Our group s research interests relating to canola meal 1. Adding value to domestic feeds through further processing Particular interest in fractionation technologies

Creating value-added products from canola meal Fractionation technology may offer opportunity to create several products from a single commodity e.g., could produce separate canola meal fractions geared toward monogastics ( fiber, CP) and ruminants ( fiber, CP) After trying several fractionation strategies, air classification permitted best separation of fiber between CM fractions

Our group s research interests relating to canola meal 1. Adding value to domestic feeds through further processing Particular interest in fractionation technologies 2. Gathering information about feeding value of domestically-produced crops and bioenergy co-products All canola meals are not necessarily created equal

Variation in oil extraction processes = different CM?? Traditional crushing plants maximize oil yield through a combination of pressing and solvent extraction Some recently-added crushing capacity employs less capital-intensive systems double press homemade

Conventional canola crushing (pre-press solvent extraction) Source: Canola Council of Canada

Double press process (omits solvent extraction) Source: Canola Council of Canada

Alternative extraction processes (small scale, low capital)

Table 1. Analyzed nutrient content of canola meal samples and fractions test compared in the present study. Nutrient Extruder Press CM Expeller Press CM Conventional Canola Meal ACCM Fines' fraction ACCM Coarse' fraction Moisture 7.51 5.63 7.65 6.21 6.14 Crude Protein 29.54 35.60 37.92 38.77 36.52 Total Amino Acids 23.93 32.39 34.57 33.71 32.69 Crude Fat 17.12 12.75 4.76 4.28 3.23 Crude Fiber 10.04 6.01 7.24 0.40 2.04 ADF 22.34 16.08 15.49 15.85 21.31 NDF 27.79 20.17 24.39 23.21 36.60 Calcium 0.60 0.60 1.33 0.83 1.27 Phosphorus 0.82 1.04 1.13 1.09 1.08 Lysine 1.20 2.11 2.15 2.08 2.03 Methionine 0.54 0.69 0.74 0.73 0.69 TSAA 1.24 1.55 1.63 1.60 1.51 Threonine 1.16 1.52 1.58 1.54 1.48 Tryptophan 0.39 0.52 0.59 0.55 0.49 Arginine 1.54 2.19 2.31 2.17 2.13

Objectives 1. To compare nutrient digestibility among a sample of conventional canola meal and two fractions produced by air classification 2. To compare nutrient digestibility among samples of conventional, double-pressed and extruder pressed canola meals

METHODS AND MATERIALS

Our approach Ingredient Basal diet Test diets Wheat 87.75 61.42 Canola oil 5.00 3.50 Test ingredient - 30.00 Dicalcium phosphate 2.75 1.92 Limestone 1.72 1.20 Vitamin/mineral premix 0.71 0.50 Choline chloride premix 0.71 0.50 Salt 0.57 0.40 Antibiotic 0.07 0.05 Chromic oxide 0.71 0.50

Our approach (cont d) Commercial starter diet Test diets d0 d14 d21

Experimental design Cage (13 birds/pen) = experimental unit Digesta and excreta were pooled to produce one sample of each per pen Randomized complete block design Each treatment appeared once in each of 6 blocks for 6 replicate cages per treatment

Measurements Feed disappearance measured over the experimental period Body weight on d 14 and d 21 Diets, ingredients, digesta and excreta assayed for DM, Cr, CP and GE, P and Ca Full AA profile also developed for diets and digesta ADF, NDF, CF and EE for diets

Statistical analysis Nutrient digestibility coefficients compared using PROC MIXED of SAS (v 9.2) Main effect = test ingredients Random term = block Covariates tested = ADFI; intake of ADF, NDF, CF and respective nutrient Preplanned contrasts: Between oil extraction processes Fractions vs. parent stock

RESULTS

Table 2. Nutrient digestibility of conventional canola meal compared to fine and coarse air classified fractions Conv. CM AC fine fraction AC coarse fraction SEM P - value AME, kcal/kg 76.87 a 45.51 b 48.93 b 3.58 < 0.0001 Dig Lysine 88.66 85.89 87.35 1.35 0.2280 Dig Methionine 98.34 a 92.53 b 95.48 ab 1.35 0.0166 Dig TSAA 93.59 a 85.33 b 87.53 b 1.84 0.0047 Dig Threonine 82.74 a 74.41 b 79.11 ab 2.10 0.0311 Dig Tryptophan 85.07 81.40 82.43 1.28 0.0577 Dig Arginine 94.91 94.48 96.37 0.76 0.5907 Dig Total AA 92.56 a 86.22 b 89.66 ab 1.32 0.0098

Table 3. Digestible nutrient content of conventional canola meal compared to fine and coarse air classified fractions, as-fed basis AC fine AC coarse Conv. CM SEM P - value fraction fraction AME, kcal 3338 a 2056 b 2136 b 161 < 0.0001 Dig Lys, % 1.91 a 1.79 b 1.78 b 0.03 0.0008 Dig Met, % 0.73 a 0.68 b 0.66 b 0.01 < 0.0001 Dig TSAA, % 1.53 a 1.37 b 1.32 b 0.03 < 0.0001 Dig Thr, % 1.31 a 1.15 b 1.17 b 0.03 0.001 Dig Trp, % 0.50 a 0.45 b 0.40 c 0.01 < 0.0001 Dig Arg, % 2.19 a 2.05 b 2.05 b 0.02 < 0.0001 Dig Total AA,% 32.00 a 29.07 b 29.31 b 0.42 < 0.0001

Table 4. Nutrient digestibility of samples of conventional, doublepressed and extruder pressed canola meal Conv. CM Double pressed CM Extruder pressed CM SEM P - value ATTD GE 76.87 a 56.73 b 67.77 a 3.58 0.0011 AID Lysine 88.66 b 72.52 c 97.33 a 1.35 0.0339 AID Methionine 98.34 a 81.43 c 91.37 b 1.35 <0.0001 AID TSAA 93.59 a 75.79 c 84.92 b 1.84 <0.0001 AID Threonine 85.07 b 85.08 b 88.86 a 1.28 0.0052 AID Tryptophan 82.74 a 67.44 b 85.80 a 2.10 0.0278 AID Arginine 94.91 a 85.41 b 97.13 a 0.76 0.0009 AID Total AA 92.56 a 75.22 c 84.83 b 1.32 <0.0001

Table 5. Digestible nutrient content of samples of conventional doublepressed and extruder pressed canola meal Conv. CM Doublepressed CM Extruderpressed CM SEM P - value AME, kcal/kg 3338 a 2837 b 3344 a 161 <0.0001 Dig Lys, % 1.91 b 0.87 c 2.05 a 0.03 <0.0001 Dig Met, % 0.73 a 0.44 c 0.63 b 0.01 <0.0001 Dig TSAA, % 1.53 a 0.94 c 1.32 b 0.03 <0.0001 Dig Thr, % 1.31 a 0.78 b 1.30 a 0.03 <0.0001 Dig Trp, % 0.50 a 0.33 c 0.46 b 0.01 <0.0001 Dig Arg, % 2.19 a 1.32 c 2.13 b 0.02 <0.0001 Dig Total AA,% 32.00 a 18.00 c 27.48 b 0.42 <0.0001

Conclusions Despite successfully separating CM into two fractions differing in crude fiber, no real impact on digestible nutrient content Value of AC fractionating CM?? Extraction process has a large impact on digestible nutrient content of resulting meal Importance of knowing origin of the CM Meal from low-capital crushing facilities can still be of excellent feeding value

Acknowledgements Funding: Technical assistance: Emily Johnson Kerry Nadeau Staff and students at PRC