Supplemental Material: Annu. Rev. Microbiol. 2016. 70:179 98 doi: 10.1146/annurev-micro-102215-095539 Molecular Genetic Analysis of Chlamydia Species Sixt and Valdivia Supplemental Table 1: Shuttle vectors for Chlamydia Vector name ppbw100 pbr325::l2 pgfp::sw2 pgfp-cat::sw2 IncDProm-RSGFP- IncDTerm IncDPrommCherry-IncDTerm IncDProm-CFP- IncDTerm pcte1 (C. trachomatis E/UW-5/CX) CDS1 and CDS3 L2/434/Bu) CDS1 Shuttle vectors for Chlamydia trachomatis (Kanamycin) none none First successful but transient transformation of Chlamydia First stable transformation of Chlamydia First stable transformation of Chlamydia with a vector conferring expression of a fluorescent protein Derivative of pgfp::sw2 lacking ß-lactamase gene; first description of chloramphenicol for selection in Chlamydia NA b (12) 13494 (16) 11539 (16) 10676 (19) none 9184 (1) rsgfp (C. trachomatis incd promoter and mcherry (C. trachomatis incd promoter and CFP (C. trachomatis incd promoter and Derivative of expressing rsgfp Derivative of expressing mcherry Derivative of expressing CFP 10293 (1) 10272 (1) 10281 (1)
pbrct pbra pgfpbsd/z::sw2 pask-gfp-l2 pask- GFP/mKate2-L2 ptl2-tap puc-pcl2 pdnak-rogfp2- SW2 pbomb4 pbomb4-mci L2/434/Bu) all ORFs pcta (C. trachomatis A2497) all ORFs E/SW2) CDS1 E/SW2) CDS1 none 11578 (10) none Derivative of pbrct containing from C. trachomatis A Blasticidin (Zeocin) GFP-BSD GFP (teta promoter, inducible) GFP (teta promoter, inducible); mkate2 (weakened C. trachomatis dnak First description of blasticidin S for selection in Chlamydia First description of inducible gene expression in Chlamydia Derivative of pask-gfp-l2 with constitutive expression of mkate2 none Derivative of pask-gfp-l2 for inducible expression of TAPtagged proteins none First description of dendrimerenabled transformation of C. trachomatis rogfp2 (C. trachomatis dnak rsgpp mcherry Designed to monitor the redox state in the cytosol of Chlamydia MCS containing BamHI, SacII, NotI, NheI, PstI, AgeI, KpnI, and SalI Derivative of pbomb4 expressing mcherry 11559 (11) NA b (5) 11439 (18) 12124 (18) NA b (2) NA b (7) NA b (14) 10556 (3) NA b (3)
pbomb4r rsgfp Derivative of pbomb4 with C. trachomatis rpob promoter upstream of MCS 10755 (3) pbomb4r-mci pbomb4-tetmcherry pbomb3 pct308-gfp pl2dest mcherry rsgfp ; mcherry (tetracycline inducible (tetracycline inducible mcherry Derivative of pbomb4-mcl with C. trachomatis rpob promoter upstream of MCS Derivative of pbomb4 with tetracycline inducible promoter in MCS NA b (3) 12003 (3) Derivative of pbomb4 11210 (17) 11941 (13) 11931 (9) Vector name pbrcm pcm (C. muridarum) all ORFs Shuttle vectors for Chlamydia muridarum none Derivative of pbrct containing from C. muridarum 11339 (11)
pgfp::cm pcm (C. muridarum Nigg) all ORFs Derivative of pgfp::sw2 containing from C. muridarum NA b (8) pgfp-luc-cm psw2niggcds2 pcm (C. muridarum Nigg) all ORFs ; CDS2 replaced by CDS2 from C. muridarum pnigg ; Luciferase (C. muridarum pgp4 promoter, C. trachomatis D CT579 Derivative of pgfp::cm expressing luciferase Originates from recombination between pgfp::sw2 and the endogenous from C. muridarum Nigg NA b (4) 11536 (15) Vector name pcl2-eg(kg) Shuttle vectors for Chlamydia pneumoniae (URA3, LEU2-d) none Chlamydia-yeast shuttle vector; no antibiotic selection marker for Chlamydia, first description of dendrimer-enabled transformation of Chlamydia with a shuttle vector NA b (6) a Selection markers in parentheses were used for selection in E.coli or S. cerevisiae, but not Chlamydia b NA refers to not available (information not given in the reference)
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