Healthy cereal-based snacks from byproducts of the malting and fruit processing industries. Eimear Gallagher Teagasc Food Research Centre, Ashtown

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Healthy cereal-based snacks from byproducts of the malting and fruit processing industries Eimear Gallagher Teagasc Food Research Centre, Ashtown

Background to the project Millions of tonnes of by-products generated from food processing Much is disposed of (costly) or used as landfill Proven to be valuable sources of healthy compounds Aim of the project To utilise nutritious by-products of food processing to generate novel, healthy, cereal-based snacks

Benefits of fruit by-products as a food ingredient: Potential nutritional benefits dietary fibre and bioactivity. Potential technical benefits bulking agent, water binding, gelling, fat replacer. By-products: Brewer s Spent Grain (BSG) Apple Pomace Orange Pomace

BSG: Kilned & milled Apple pomace and orange pomace: Freeze-dried & milled Treatment of the by-products Also currently working with fresh, frozen samples to eliminate the cost of freezedrying

1. Chemical characterization of the milled by-products Moisture (Weight difference, AOAC 925.10) Total Dietary Fibre (Amylase/protease/Amyloglucosidase) Polysaccharides (Saeman hydrolysis) Ash (Weight difference, AOAC 923.03) Micro Phenolic compounds (Solid-liquid extraction) Protein (Kjeldhal, AOAC 920.87) Protein digestibility (Onyango et al., 2004) Starch (Amylase/Amyloglucosidase) Fat (Weight difference) Antioxidant capacity (FRAP/DPPH) Starch digestibility (Onyango et al., 2004)

2. Functional and rheological characterization of the milled by-products Water hydration capacity Swelling capacity Particle size Colour (L*a*b*) Water holding capacity Oil holding capacity Starch pasting properties Fundamental rheology

3. Baking and extrusion trials Baked Extruded Gluten free

1. Characterization of the milled by-products 25 Protein Protein % 20 15 10 5 0 Wheat flour AP BSG Sample Fibre 80 Fibre % 60 40 20 0 Wheat flour AP BSG Samples

1. Characterization of the milled by-products Composition Fat (%) DM Ash (%) DM Carbohydrate (%) Starch (%) Calcium (mg/l) Potassium (mg/l) Magnesium (mg/l) Zinc (mg/l) Iron (mg/l) Pectin (DW) Apple Pomace 2.53 +/- 0.26 1.79 +/- 0.02 84.76 +/- 0.56 5.6 +/- 0.0 1265 +/- 142 2531 +/- 284 126 +/- 14.22 1.66 +/- 0.62 8.39 +/- 1.22 8.04 +/-0.08 Orange Pomace 2.14 +/- 0.33 4.18 +/- 0.08 77.78 +/- 0.67 3.4 +/- 0.0 4112 +/- 414 4112 +/- 414 570 +/- 102 2.28 +/- 0.41 9.76 +/- 0.88 18.69 +/-0.65

Scanning electron microscopy of the flours Apple pomace Orange pomace BSG

3. Baking and extrusion trials Breadsticks (15, 25, and 35 % BSG) Crispy slices (0, 15, and 25 % BSG) Texture, volume, colour, rheology, crumb structure, shelf-life Extruded snacks (5-10% apple pomace) Texture, volume, colour, crumb structure, shelf-life Gluten-free bread (2-8% orange pomace) Texture, volume, colour, crumb structure, shelf-life, sensory, staling properties (DSC-Microscopy) Scones (10, 20, 30 % apple pomace) Texture, colour, crumb structure, shelf-life, volatile analysis (flavour analysis) Bread rolls (Pup-loaves) (10, 15 and 20 % BSG) Texture, volume, colour, shelf-life, sensory Texture, puffing, structure, sensory Cake containing orange pomace and reduced levels of fat Texture, volume, colour, crumb structure, shelf-life Protein Fibre Antioxidants..

Baked Breadsticks Biscuits Crispy slices Cake Biscotti Brioche Bread rolls Scones

Rice Corn Soya flour Wheat semolina BSG & Apple Pomace Extruded

Breads Biscuits Puffed snacks Orange & Apple Pomace Gluten free

Baking Trials - BSG Breadsticks Baking & Extrusion Trials - Apple Pomace & Orange pomace Gluten-free bread Puffed Snacks

Crispy properties of breadsticks No of peaks Area Linear distance Crispiness Index (Ci) Crispiness Work (Wc) Average peak force Loss of crispiness with highest levels of BSG 10% BSG addition did not alter texture (crispiness) of the samples

Baking Trials (BSG) Main outcomes: 90-day shelf life trial for breadsticks (15-35% inclusion) 90 80 Hardness of breadsticks 70 Hardness (N) 60 50 40 30 20 10 0 0 10 20 30 40 50 60 70 Storage days 0%BSG 15%BSG 25%BSG 35%BSG 80 90

Sensory acceptability of breadsticks Panellists distinguished between all samples based on the aroma 10% BSG-containing snacks equally acceptable as the control Texture and taste most important attributes

Fibre and antioxidant capacity of breadsticks Fibre Fibre % 20 15 10 5 0 Control 10%BSG 15%BSG 25%BSG Sample Antioxidant capacity

Gluten-free bread trial (Orange pomace) We investigated: Level of pomace addition (0-8%) Dough proofing times (35-50 min) Water addition (85-100%) Response surface design & validation We measured: Dough rheology, loaf volume, crumb grain, crumb structure and microstructure, texture, sensory properties

Loaf volume Crumb hardness

Optimisation of gluten-free bread formulation 5.5% orange pomace addition 94.6% water addition 49 min proofing time Control & Optimised bread Microstructure Sensory analysis Doubled dietary fibre content

Puffed snacks / extrusion trial (Apple pomace) Extrusion: Creates a high temperature, high pressure and shear force environment Modifies moistened expansible starchy material to produce a puffed endproduct Products such as breakfast cereals, flat breads and puffed snacks We investigated: Apple pomace (0-10%) + corn flour (blend) Die head temperature (150-200oC) Screw speed (60-100 RPM) We measured: Expansion ratio, bulk density, porosity, moisture. Texture and Acoustic Characteristics hardness, acoustic energy

Optimisation of puffed snack formulation Apple Pomace addition: Affected the texture and acoustic properties Decreased hardness Increased crispiness Excess AP detrimental effects on extrudate characteristics Optimised formulation and process: 7.7% apple pomace 69 RPM screw speed 150oC die head temperature

Many thanks to Norah O Shea Thank you! Anastasia Ktenioudaki Sofia Reis Emily Crofton Eimear.Gallagher@teagasc.ie Tel: (01) 805 9500