Labratry f Fd Chemistry and Bichemistry Leuven Fd Science and Nutritin Research Centre Functinal starch: A better use f starch in fds Jung-Sun Hng, Ph.D Brussels, Apr 25 2015
Jung Sun Hng Summary Fd chemistry, carbhydrates/starch chemistry A specialist in mdificatin techniques f starch Chemical, physical, and enzymatic methd Fr specific applicatin in fd system and industrial purpses A strng research backgrund in fundamental aspect f carbhydrates plymers/starches and their derivatives Educatin Ph.D., Schl f Fd Science, University f Idah, Mscw, Idah, US. Jan 2013 M.S., 서울대학교식품생명공학과 (Enzyme technlgy) Feb 2008 B.S., 동국대학교식품공학과 Feb 2006 2
Current affiliatin Department f Micrbial and Mlecular Systems Centre fr Fd and Micrbial Technlgy Labratry f Fd Chemistry and Bichemistry Leuven Fd Science and Nutritin Research Centre (LFRCe) Labratry f Fd Technlgy Labratry f Fd Micrbilgy Labratry f Malting and Brewing Sciences 3
Lab LFRCe Missin T generate basic insights in Structure and prperties f cereal cnstituents Starch Nn-starch plysaccharides Strage prteins Enzymes Lipid T imprve prcessing, final prducts and/r hearth related functinality 4
Starch? Energy strage frm in plants The mst imprtant carbhydrate in the human diet Abundant surce f functinal biplymers Thickeners and stabilizers in fd and nn-fd prducts Bidegradable cmpnents in numerus chemical applicatins (e.g., plastics, detergents, glues etc.) Barley Izydrczyk et al (2014) 5
Starch applicatin EU - 2013 Ttal Market: 9 millin tnes Cnfectinary and drink (32%) Prcessed fds (29%) Crrugating and paper making (29%) Pharma & Chemicals (5%) Eurpean starch industry assciatin (aaf) 6
Extractin f starch frm plants wheat Milling Islatin Kernel (100%) Flur (76-78%) Starch (58-60%) whle wheat flur Bran 7
Starch granules Scanning electrn micrscpy crn (48%) wheat (39%) ptat (13%) rice banana pea 8
Starch in Fds Fd thickener Slid Liquid Crispy Hard Chewy Flppy Sft Thick Fluidly 9
Starch in Fds Phase transitin 95 C H 2 O 50 C H 2 O 5 C Gelatinizatin Retrgradatin Gelatin temperature viscsity 10
Visc (RVU) Functinal fd starch 600 500 400 300 200 100 0 65% Ethanl Aqueus-Na 2 SO 4 Native Aqueus 65% acetne 100 80 60 40 20 Temp (C) T btain functinal starch, Need additinal prcess (mdificatin) t enhance functinality f starch using Chemicals Enzymes Physical treatments Dual/multiple prcesses 0 5 10 15 20 Time (min) Viscsiy f 7% (w/v) native starch and crss-linked starch in 92.5% (w/w) DMSO-water slutin (Hng et al., 2015) 11
Functinal fd starch 80% f starch in markets is mdified! Fat Replacer/Fat Mimetic Lw-fat butter spread/margarine Lw fat maynnaise Lw fat milk type prducts Lw -fat ice cream Texture Imprvers Imprve bread texture Crispness f crackers and biscuits Viscsity breakdwn resistant fr can fds Desired chewiness fr extruded prducts Functinal fiber Slw digestible ckies Resistant starch muffin Encapsulatin f flavr/il Eurpean starch industry assciatin (aaf) Abbas et al. 2010 12
Functinal fd starch Prspects Chemical levels Effect f starch mdificatin Precise cntrl f the mdificatin prcess Advanced techniques t prduce functinal starches Better understanding f starch structure and reactin patterns Nvel functinality f fd starches Clean labeled fd starch 13
Starch structure Micrscpic view Scanning electrn (SEM) Starch granule (X10-5 ) Grwth ring (X10-6 ) Pwder (X1) Objective Cnfcal laser scanning (CLSM) Wax Glass slide Flurescence* prbed starch granule Cavity Channel 14
Starch structure Amylse and Amylpectin Cluster B1 chain B2 chains Plant pathlgy nline 15
Starch structure Frm granules t mlecules Granular level Amylse Mlecular level C chain B chain A chain cluster Amylpectin Jenkins & Dnald, 1995 16
Prpsed mdel fr starch mlecules rganizatin Based n reactivities f starch chains IM IM Cluster mdel (traditinal mdel) Backbne structure mdel (alternative mdel) Hng & Huber (2015) 17
Overall research gal & apprach Mdel reactin system Flurescent prbed starch derivatives Granular Reactin Patterns Cnfcal Laser Scanning Micrscpy Bridging the Gap Mlecular Reactin Patterns Size Exclusin Chrmatgraphy - Refractive Index (CHO) - Flurescence (extent f reactin) Mdified Starch Prperties Rapid Visc Analyzer 18
Cnclusins Starch granular architecture impacts reactivities f starch chains mlecules. Granular/mlecular reactin patterns impact physical prperties f mdified starches. By cntrlling reactin parameters, Design f reactin pattern A minimal level f reagent additin Starch mdel reactin apprach: prvides insight beynd current understanding f starch structure. 19
Carbhydrates and Health Tell me what yu eat, and I will tell what yu are. - Jean Anthelme Brillat-Savarin The relatinship between prtein:carbhydrate dietary Intake vs. lifespan in Mice (Simpsn et al., 2014) Mar 2015 The quality f carbhydrates is imprtant! 20