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Supplemental Figure I Kl ( mmol/l)-induced Force orta M (mn) 1 (mn) 1 Supplemental Figure I. Kl-induced contractions. and, Kl ( mmol/l)-induced contractions of the aorta () and those of mesenteric arteries (M) (). n = 7 animals per group. Results are expressed as means±sem.

Supplemental Figure II - 1 1 orta 9 7 SNP (- log mol/l) - 1 1 M 9 7 SNP (- log mol/l) - D - 1 7 NS119 (- log mol/l) 1 7 NS119 (- log mol/l) E - F - 1 (n=) (n=) (n=) 7 3 1 (n=) (n=) (n=) 7 3 Supplemental Figure II. Endothelium-independent relaxations. -F, Endothelium-independent relaxations of aorta (, and E) and mesenteric arteries (M) (, D and F) to sodium nitroprusside (SNP) ( and ), NS-119 ( and D) and hydrogen peroxide (H O ) (E and F) are shown. n = -7 animals per group. The responses to H O were examined in the presence of indomethacin (1 - mol/l) and N -nitro-l-arginine (L-NN; 1 - mol/l). Values are expressed as means±sem. P<., P<.1 vs..

Supplemental Figure III orta M - - 1 control ODQ 7 3 1 control ODQ 7 3 - D - E 1 - control ODQ 7 3 F 1 - control ODQ 7 3 control control 1 ODQ 7 3 1 ODQ 7 3 Supplemental Figure III. Effects of sg inhibition on H O -induced relaxations. -F, The responses of the aorta (, and E) and mesenteric arteires (M) (, D and F) to exogenous hydrogen peroxide (H O ) were examined in the presence or absence of a soluble guanylate cyclase (sg) inhibitor 1H-[1,,]oxadiazolo[,3-a]quinoxalin-1-one (ODQ, mol/l). n = 7 animals per group. Values are expressed as means±sem. P<., P<.1 vs. control.

Supplemental Figure IV Tie promotor anine av-1 cdn Poly -enhancer NotI KpnI XmaI SalI Endothelium-specific av-1-tg +/- av-1 -/- Endothelium-specific av-1-tg +/-, av-1 +/- av-1 -/- Endothelium-specific av-1-tg +/-, av-1 -/- (av-1-r) av-1-r av-1 D31 av-1-r av-1 D31 Supplemental Figure IV. Endothelium-specific av-1 reconstitution on background., The construct used to generate endothelium-specific av-1 transgenic mice. The canine av-1 cdn (37 bp) was driven under the endothelium-specific promotor, murine Tie promotor. Endothelium-specific av-1-reconstituted (av-1-r) mice were generated by crossing endothelium-specific av-1 transgenic mice with mice. and, Immunostaining images for av-1 and D31 (an endothelial cell marker) of the aorta () and mesenteric arteries (). Scale bars = m.

Supplemental Figure V - orta (n=) - M (n=) E 1 1-1 - (n=) av-1-r (n=) 9 7 SNP (- log mol/l) (n=) (n=) av-1-r (n=) 7 NS119 (- log mol/l) (n=) (n=) av-1-r (n=) D F 1 1-1 - 9 7 SNP (- log mol/l) (n=) (n=) av-1-r (n=) 7 NS119 (- log mol/l) (n=) (n=) av-1-r (n=) (n=) av-1-r (n=) 1 7 3 1 7 3 Supplemental Figure V. Endothelium-independent relaxations. -F, Endothelium-independent relaxations of the aorta (, and E) and mesenteric arteries (M) (, D and F) to sodium nitroprusside (SNP) ( and ), NS-119 ( and D) and hydrogen peroxide (H O ) (E and F) are shown. n = animals per group. The responses to H O were examined in the presence of indomethacin (1 - mol/l) and N w -nitro-l-arginine (L-NN; 1 - mol/l). Values are expressed as means±sem. P<., P<.1 vs..

Supplemental Figure VI (mn) PE L-NN Force ( mn) Force 1 1 3 1 (mn) 1 PE L-NN Time (min) Force D ( mn) Force Time (min) Supplemental Figure VI. asal NO release. -D, Representative traces and quantitative analysis of contractile responses evoked by L- NN (1 - mol/l) in rings precontracted with a submaximal dose of phenylephrine (PE; 1-7 mol/l) from aorta ( and ) and mesenteric arteries ( and D). n = 7 animals per group. Values are expressed as means±sem. P<., P<.1.

Supplemental Figure VII (mg/g) 1 Heart weight/body weight (n=) (n=) (n=) (mmhg) 1 1 week weeks Systolic blood pressure weeks (bpm) Heart rate (n=1) (n=1) (n=11) sham (n=) sham (n=) sham (n=) 11 1 9 7 Time (weeks) Time (weeks) Supplemental Figure VII. Effects of cardiac pressure-overload on survival and hemodynamic parameters. -, Time course of heart weight/body weight changes (n = per group) (), systolic blood pressure () and heart rate () (n = 11-1 animals per -group, n = animals per sham-group) after transverse aortic constriction () or sham operations. Values are expressed as means±sem. P<., P<.1 vs. at each time point, P<., P<.1 vs. sham.

Supplemental Figure VIII (n=-) (n=-) (n=-) ( m ) Myocyte cross-sectional area 3 1 1 week weeks weeks D (%) Perivascular fibrosis 1 1 1 week weeks weeks E F (%) 1 Interstitial fibrosis 1 week weeks weeks

Supplemental Figure VIII Supplemental Figure VIII. Histological analysis of cardiac hypertrophy and fibrosis., Representative images of hematoxylin-eosin staining of the myocardium weeks after or sham operations., Quantitative analysis of cross-sectional area of cardiomyocytes. and E, Representative images of Masson-trichrome staining for evaluation of perivascular () and interstitial (E) fibrosis in the hearts after weeks of or sham operations. D and F, Quantitative analysis of perivascular (D) and interstitial (F) fibrosis. n = - animals per group. Scale bars = 1 m. Values are expressed as means±sem. P<., P<.1 vs. at each time point, P<., P<.1 vs. sham.

aveolin-1 enos aveola O L-rg L-it O - O gonist R a + /am O O - a + /am enos L-it gonist R O L-rg Supplemental Figure IX aveolin-1 enos O O - O L-rg L-it gonist R a + /am enos L-it O L-rg u,zn-sod H O NO NO H O H O NO Endothelium K + K a sg sg PKG1 dimerization Hyperpolarization cgmp Relaxation Tolerance cgmp LV pressure overload VSM LV systolic function, FR, Myocardial hypoxia Supplemental Figure IX. Summary of the present study. Summary of endothelium-dependent and -independent relaxations of small mesenteric arteries and coronary microvessels (upper panel) and the effects of cardiac pressure-overload (lower panel) are illustrated. oth av-1 deficiency and enos overexpression lead to over-activation of enos and excessive production of NO, causing disruption of the physiological balance between NO and EDH in microcirculations. s compared with mice, both and mice showed reduced survival after chronic cardiac pressure-overload induced by, associated with accelerated left ventricular systolic dysfunction, reduced coronary flow reserve and enhanced myocardial hypoxia. a + /am denotes calcium/calmodulin; FR, coronary flow reserve; u,zn-sod, copper, zinc-superoxide dismutase; K a, calcium-activated potassium channels; L-rg, L-arginine; L-it, L-citrulline; LV, left ventricular; PKG1a, protein kinase G1a; R, receptor; sg, soluble guanylate cyclase; VSM, vascular smooth muscle cell.

Godo et al., Page 11 Supplemental Tables Supplemental Table I. aseline haracteristics and Echocardiographic Parameters. (n) (n) (n) aseline characteristics ge, week-old 1.±.7 (17) 1.3±.7 (17) 1.9±.7 (17) ody weight, g 7.±. (17).±.7 (17) 7.1±. (17) Heart weight, mg 1±3 (17) 1± (17) 1±3 (17) Heart weight/body weight, mg/g.±.3 (17).±. (17).±. (17) Systolic blood pressure, mmhg 11±3 () 1±3 () 3±1 () Heart rate, bpm 73±9 () 9±3 () 9±1 () Plasma nitrite/nitrate, mol/l.3±7.9 (17) 19.7±17.9 (17) 1.±17.9 (17) Echocardiography Heart rate, bpm# 73± (1) 9±7 (1) 9±9 (1) LVDd, mm 3.7±. (1) 3.±.7 (1) 3.3±. (1) LVDs, mm.3±. (1).17±. (1).3±. (1) LVFS, % 37.±1. (1) 3.9±1. (1) 3.±1. (1) IVST, mm.79±. (1).9±. (1).7±.3 (1) P, mm.73±.1 (1).7±. (1).77±. (1) Results are expressed as means±sem. bpm denotes beats per minute;, caveolin-1-knockout mice;, endothelium-specific endothelial nitric oxide synthase transgenic mice; IVST, interventricular septal thickness; LVDd, left ventricular (LV) end-diastolic diameter; LVDs, LV end-systolic diameter; LVFS, LV fractional shortening; P, posterior wall thickness;, wild-type mice. P<., P<.1 vs.. Under conscious conditions. #Under light anesthesia with.-.% isoflurane.

Godo et al., Page 1 Supplemental Table II. Precontraction Forces, E Values and Maximal Relaxations of orta and Mesenteric rteries gonist Precon. Vessel (mn) (n) SNP E Max. Precon. E (-log mol/l) Relax. (mn) (-log mol/l) (%) Max. Precon. Relax. (mn) (%) E Max. (-log mol/l) Relax. (%) orta (7).±..±.1 97.±1. 7.±.3.1±. 93.±.9.7±. 7.3±. 7.±. M (7) 7.±.3 7.±. 97.±.3 7.7±..1±.1 99.3±..3±..7±. 79.±. NS-119 orta (7).±..±..±. 7.±..±.1 9.3±1.9.±.1.±.1.±1. M (7).±..±.1 9.±. 7.±.9.3±.1 9.3±..9±..±.1 9.±. H O orta () 11.±..±.1 7.±. 1.9±.7.±.1.3±1. 1.9±..3±.1 73.±.1 M () 7.±.7.±.1 9.1±..1±.9.±.1 9.3±3. 7.±..±.1 9.±1.1 Results are expressed as means±sem. indicates caveolin-1-knockout mice;, endothelium-specific endothelial nitric oxide synthase transgenic mice; E, half-maximal effective concentration; HO, hydrogen peroxide; M, mesenteric artery; Max. Relax., maximal relaxation; Precon., precontraction force to phenylephrine (1 - mol/l); SNP, sodium nitroprusside;, wild-type mice. P<., P<.1 vs.. In the presence indomethacin (1 - mol/l) and L-NN (1 - mol/l).

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