Immunohistochemistry Research Products Multiple Tissue Arrays (MTA) Primary and Secondary Antibodies Detection Systems Antigen Retrieval Solutions Tissue Processing Fixatives BD Biosciences Clontech Discovery Labware Immunocytometry Systems PharMingen BD Biosciences
Introduction BD Biosciences PharMingen is proud to introduce an expanded line of immunohistochemistry/immunopathology research reagents. This expanded line of reagents consists of primary and secondary antibodies as well as a line of antigen retrieval and detection systems optimized for your research immunohistochemistry needs. In addition, we are offering a series of Multiple Tissue Arrays (MTA) of human, mouse and rat organ tissues. Quality Affinity purified monoclonal antibodies optimised for IHC. Performance analysed in Zinc- and formalin-fixed paraffin embedded tissues with antigen retrieval as well as acetone-fixed frozen sections. Easier to Use Antibodies are conveniently packaged in a 1 ml format. We ve matched the titers so you can make the same dilutions and always be in the right test range. Complete Portfolio Over 250 primary and secondary antibodies, antigen retrieval solutions, fixatives, kits, detection systems, and multiple tissue arrays (MTA). Multiple Tissue Arrays (MTA) Multiple tissue arrays allow for high throughput screening by immunohistochemistry and In-Situ hybridization. Our tissue arrays are made with formalin-fixed or Zinc-fixed paraffin embedded tissues. Zinc fixative provides better morphology than a frozen section, yet still preserves the antigenicity of a large number of cell markers (approximately 75-80% of human and mouse CD markers tested were reactive with Zinc fixed paraffin embedded tissues). Our normal MTA includes, but is not limited to, tissues from heart, lung, liver, spleen, kidney, lymph node, thymus, thyroid, pancreas, skin, adrenal gland, artery, vein, cerebrum, cerebellum, skeletal muscle, colon, stomach, small intestine, salivary gland, and reproductive systems. Key features of multiple tissue arrays: A broad spectrum of tissues from 24 major organs (in duplicate); Ensured retention of local tissue structure: each tissue spot is 1.5 mm in diameter to depict morphological detail; Both Zinc- and formalin-fixed paraffin embedded human, mouse and rat MTA are available. Main benefits of multiple tissue arrays: High throughput screening; Protein or nucleic acid localization; Multiple tissues in one slide for easy use and comparison; Genomics and proteomics research; Minimize workload yet maximize output. Double color immunostaining for BrdU (black nuclear staining) and CD45/B220 (red membrane staining). Inset is isotype control. Kits BrdU In-Situ Detection Kit Designed for immunohistochemical detection of BrdU and simultaneous multicolor immunostaining of intracellular and/or surface antigens. Ig HRP Detection kits The four different Ig HRP detection kits provide all reagents including biotinylated-secondary antibody, antibody diluent, ultra-sensitive, ready-to-use Streptavidin-HRP together with DAB substrate for IHC staining and immunoblotting. Specificity Application Cat. No. Anti-Hamster Ig HRP detection kit Fr, F, Zn 551012 Anti-mouse Ig HRP detection kit Fr, F, Zn 551011 Anti-rabbit Ig HRP detection kit Fr, F, Zn 551010 Anti-rat Ig HRP detection kit Fr, F, Zn 551013 BrdU In-Situ detection kit Fr, F, Zn 550803 AEC New Fuchsin BCIP/NBT DAB-Orange Detection systems and substrates DAB, AEC, BCIP/NBT, BCIP/INT, Fast-Red, New Fuchsin, DAB-Black, DAB-Orange, TMB are all substrates that can be used with either HRP or AKP enzyme detection systems. Use them in combination to perform simultaneous multi-color immunostaining of multiple antigens. Specificity Application Cat. No. AEC substrate kit Fr, F, Zn 551015 BCIP/INT Substrate kit Fr, F, Zn 551229 BCIP/NBT Substrate kit Fr, F, Zn 551009 DAB substrate Fr, F, Zn 550880 Fast-Red Substrate kit Fr, F, Zn 551014 New Fuchsin Substrate kit Fr, F, Zn 551016 Streptavidin-AKP Pre-diluted Fr, F, Zn 551008 Streptavidin-HRP Pre-diluted Fr, F, Zn 550946 2
Buffers and Fixatives Antigen Retrieval Systems BD Retrievagen A, B and TUF (Tissue Unmasking Fluid) are designed for antigen retrieval in formalin-fixed paraffin sections while maintaining good tissue morphology and the option for simultaneous multi-color immunostaining of various antigens. Zinc Fixative Zinc fixative is milder than formalin, thus less detrimental to antigenicity. Zinc fixative also maintains good tissue morphology while helping mitigate problems of epitope masking. Antibody Diluents The IHC antibody diluent is a buffered solution ideal for dilution of primary and secondary antibodies. All antibodies for IHC are provided in this IHC antibody diluent. The Immunocytochemistry diluent is useful for staining of intra-cellular antigens. CD23 staining in Zinc-fixed human tonsil. Specificity Application Cat. No. Antibody Diluent for IHC Fr, F, Zn 559148 BD Retrievagen A (ph 6.0) F, Zn 550524 BD Retrievagen B (ph 9.5) F, Zn 550527 BrdU solution 550891 Immunocytochemistry Diluent Buffer ICC 550009 Immunocytochemistry Fixation Buffer ICC 550010 IHC Zinc Fixative Zn 550523 Target Unmasking Fluid (TUF) 1x F, Zn 556605 Target Unmasking Fluid (TUF) 3x F, Zn 556606 CD4 staining in Zinc-fixed human tonsil. Multiple Tissue Arrays (MTA) Specificity Application Cat. No. Human Normal Multiple Tissue Array (MTA) F 551253 Human Normal Multiple Tissue Array (MTA) Zn 551255 Mouse Normal Multiple Tissue Array (MTA) F 551260 Mouse Normal Multiple Tissue Array (MTA) Zn 551261 Rat Normal Multiple Tissue Array (MTA) F 551257 Rat Normal Multiple Tissue Array (MTA) Zn 551259 Ki-67 staining without antigen retrieval. Figure 1. Haemotoxylin and Eosin staining of mouse tissue array. Formalin-fixed paraffin sections of Balb/c mouse normal multiple tissue array was stained with haemotoxylin and eosin to study morphologolical detail of the tissues. Shown from left to right, top to bottom are kidney, lung, liver, brain, thymus, spleen. Ki-67 staining after pre-treatment with BD Retrievagen A (ph 6.0). Figure 2. CD45 immunostaining of mouse tissue array. Formalin-fixed paraffin sections of Balb/c mouse normal multiple tissue array was stained with antibody against mouse CD45 to study the distribution of leukocytes in the different tissues. Positive staining can be seen by the intense brown labeling of cells in the spleen, thymus and lung. Shown from left to right, top to bottom are kidney, lung, liver, brain, thymus, spleen. 3
Antibodies for Cell Surface Analysis DC-SIGN staining in human tonsil. CD34 staining in human tonsil. CD45RA staining in human tonsil. vwf staining in human tonsil. Human Specificity Isotype Clone Application Cat. No. CD1a Mouse IgG1 HI149 Fr, Zn 550366 CD3 Mouse IgG2a HIT3a Fr 550367 CD3 Mouse IgG1 UCHT1 Fr, Zn 550368 CD4 Mouse IgG1 RPA-T4 Fr, Zn 550369 CD5 Mouse IgG1 UCHT2 Fr, Zn 550370 CD7 Mouse IgG1 M-T701 Fr, Zn 550371 CD8 Mouse IgG1 HIT8a Fr, Zn 550367 CD11a Mouse IgG1 HI111 Fr 550373 CD11b Mouse IgG1 ICRF44 Fr 550374 CD11c Mouse IgG1 B-Ly6 Fr, Zn 550375 CD14 Mouse IgG2a M5E2 Fr, Zn 550376 CD15 Mouse IgM HI98 Fr, F 550382 CD16 Mouse IgG1 3G8 Fr, Zn 550383 CD19 Mouse IgG1 HIB19 Fr 550384 CD20 Mouse IgG2a H1 (FB1) Fr, F 550385 CD23 Mouse IgG1 M-L233 Fr, Zn 550386 CD28 Mouse IgG1 CD28.2 Fr 550387 CD29 Mouse IgG1 Mar4 Fr, Zn 550388 CD31 Mouse IgG1 WM59 Fr, Zn 550389 CD34 Mouse IgG1 581 Fr, F 550390 CD40 Mouse IgG1 5C3 Fr, Zn 550391 CD44 Mouse IgG2b G44-26 Fr, F* 550392 CD45RA Mouse IgG2b HI100 Fr, F* 550393 CD45RO Mouse IgG2a UCHL1 Fr, F* 550394 CD62P Mouse IgG1 AK-4 Fr 550398 CD56 Mouse IgG1 B159 Fr 550395 CD69 Mouse IgG1 FN50 Fr, Zn 550400 CD83 Mouse IgG1 HB15e Fr 550401 CD90 (Thy-1) Mouse IgG1 5E10 Fr, Zn 550402 CD95 Mouse IgG1 DX2 Fr, Zn 550403 CD106 Mouse IgG1 51-10C9 Fr, Zn 550404 CD152 Mouse IgG1 BNI3 Fr 550405 CLA Rat IgM HECA-452 Fr, F* 550407 c-erbb-2 Mouse IgG1 9G6 Fr, F* 550842 DC-SIGN Mouse IgG2b DCN46 Fr, Zn 551249 Fas ligand Mouse IgG1 G247-4 Fr, F* 550841 Mouse Specificity Isotype Clone Application Cat. No. CD3e Hamster IgG 145-2C11 Fr, Zn 550275 CD3 Hamster IgG 500A2 Fr 550277 CD4 Rat IgG2a H129.19 Fr, Zn 550278 CD4 Rat IgG2a RM4-5 Fr, Zn 550280 CD5 Rat IgG2a 53-7.3 Fr, F, Zn 550522 CD8 Rat IgG2a 53-6.7 Fr, Zn 550281 CD11a Rat IgG2a M17/4 Fr 550528 CD11b Rat IgG2b M1/70 Fr 550282 CD11c Hamster IgG HL3 Fr, Zn 550283 CD19 Rat IgG2a 1D3 Fr 550284 CD25 (IL-2R α) Rat IgGM 7D4 Fr 550529 CD29 (Integrin β1) Rat IgG2a 9EG7 Fr 550531 CD29 (Integrin β1) Hamster IgG HMb1-1 Fr 550530 CD31 Rat IgG2a MEC13.3 Fr, Zn 550274 CD34 Rat IgG2a RAM34 Fr, Zn 550537 CD40 Rat IgG2a 3/23 Fr, Zn 550285 CD44 Rat IgG2b IM7 Fr, F, Zn 550538 CD45 (LCA) Rat IgG2b 30-F11 Fr, F, Zn 550539 CD49e (Integrin α5) Rat IgG2a 5H10-27 (MFR5) Fr 550540 CD45R Rat IgG2a RA3-6B2 Fr, F, Zn 550286 CD54 Hamster IgG 3 E2 Fr, Zn 550287 CD61 (Integrin β3) Hamster IgG 2C9.G2 Fr 550541 CD62E Rat IgG2a 10E9.6 Fr 550290 CD62L Rat IgG2a Mel-14 Fr, Zn 550288 CD62P Rat IgG1 RB40.34 Fr 550289 CD86 (B7-2) Rat IgG2a GL1 Fr 550542 CD90 (Thy-1.2) Rat IgG2a 53-2.1 Fr 550543 CD102 (ICAM-2) Rat IgG2a 3C4 (mic2/4) Fr 550544 CD104 (Integrin β4) Rat IgG2a 346-11A Fr 550545 CD105 (Endoglin) Rat IgG2a MJ7/18 Fr 550546 CD106 (VCAM-1) Rat IgG2a 429 (MVCAM.A) Fr 550547 4
Antibodies for Cell Surface Analysis Mouse (continued) Specificity Isotype Clone Application Cat. No. CD144 (VE-Cadherin) Rat IgG2a 11D4.1 Fr 550548 Gr-1(ly-6G) Rat IgG2b RB6-8C5 Fr, Zn 550291 H-2Kb Mouse IgG2a AF6-88.5 Fr 550550 H-2Kd Mouse IgG2a SF1-1.1 Fr 550551 H-2kk Mouse IgG2a 36-7-5 Fr 550552 I-Aab (A beta B) Mouse IgG2a AF6-120.1 Fr 550553 I-Ad Mouse IgG2a AMS-32.1 Fr 550554 Mac-3 Rat IgG1 M3/84 Fr, F*, Zn 550292 MAdCAM-1 Rat IgG2a MECA-89 Fr 550555 MAdCAM-1 Rat IgG2a MECA-367 Fr 550556 Panendothelial antigen Rat IgG2a MECA-32 Fr 550563 PNAd (CD62L Ligand) Rat IgM MECA-79 Fr, F*, Zn 550564 αβ TCR Hamster IgG H57-597 Fr 550293 γδ TCR Hamster IgG GL3 Fr 550294 TER-119/ Rat IgG2b TER-119 Fr, F, Zn 550565 erythroid cells (Ly-76) CD3e staining in mouse spleen. Rat Specificity Isotype Clone Application Cat. No. CD3 Mouse IgG3 G4.18 Fr, F, Zn 550295 CD4 Mouse IgG2a OX-38 Fr 550297 CD4 Mouse IgG2a OX-35 Fr 550296 CD8a Mouse IgG1 OX-8 Fr, F, Zn 550298 CD11b/c Mouse IgG2a Ox-42 Fr, Zn 550299 CD31 Mouse IgG1 TLD-3A12 Fr, Zn 550300 CD45RA or A/B Mouse IgG1 OX-33 Fr 550567 CD45 (LCA) Mouse IgG1 OX-1 Fr, Zn 550566 CD45R Mouse IgG2b HIS24 Fr, F, Zn 550301 CD49a (Integrin α1) Hamster IgG Ha31/8 Fr 550568 CD54 Mouse IgG1 1A29 Fr, F*, Zn 550302 CD61 (Integrin β3) Mouse IgG1 F11 Fr, Zn 550569 CD90 (Thy-1) Mouse IgG2a HIS51 Fr, Zn 550570 CD90 (Thy-1) Mouse IgG1 OX-7 Fr, Zn 550571 Dendritic cells Mouse IgG1 OX-62 Fr, F, Zn 550303 Granulocytes Mouse IgM HIS48 Fr, F, Zn 550304 Macrophage subset Mouse IgG2a HIS36 Fr, Zn 550573 MadCAM-1 Mouse IgG1 OST2 Fr, Zn 550309 Mono phagocytes Mouse IgG1 1C7 Fr, F, Zn 550305 NKR-P1A Mouse IgG1 10/78 Fr, Zn 550306 RT1B Mouse IgG1 OX-6 Fr, F, Zn 550574 αβ TCR Mouse IgG1 R73 Fr, F, Zn 550307 γδ TCR Mouse IgG1 V65 Fr 550308 CD45 staining in mouse spleen. CD90 staining in mouse spleen. αβtcr staining in rat spleen. 5
MUC2 staining in human colon cancer. p53 staining in human breast carcinoma. MART-1 staining in human melanoma. Antibodies for Tumor Markers/Cell Biology Human Androgen receptor G122-25 Mouse IgG1 Fr, F 550849 anti-p21 SX118 Mouse IgG1 Fr, F* 550827 Bcl-2 Bcl-2/100 Mouse IgG1 Fr, F* 550847 c-myc 9E10 Mouse IgG1 Fr, F* 550836 Cyclin B1 GNS-1 Mouse IgG1 Fr, F* 550846 DCC G97-449 Mouse IgG1 Fr, F* 550845 Eosinophil AHE-2 Mouse IgG1 Fr 550843 major basic protein Eosinophil peroxidase AHE-1 Mouse IgG1 Fr 550844 HPVL1 CAMVIR-1 Mouse IgG2a Fr, F* 550840 Ki-67 B56 Mouse IgG1 Fr, F* 550609 Maspin G167-70 Mouse IgG2b F* 550839 MLH-1 G168-15 Mouse IgG1 Fr, F* 550838 MLH-2 G219-1129 Mouse IgG1 Fr, F* 550837 MMP-1 III12b Mouse IgG1 Fr, F* 551000 MMP-2 A-Gel VC2 Mouse IgG1 Fr, F* 551003 MMP-3 SL-1 IID4 Mouse IgG2b Fr, F 551117 MMP-7 ID2 Mouse IgG2b Fr, F 551004 MMP-9 IIA5 Mouse IgG1 Fr, F 551005 MMP-10 IVC5 Mouse IgG2b Fr, F 551118 MMP-11 SL3.05 Mouse IgG1 Fr, F 551006 MUC1 HMPV Mouse IgG1 Fr, F* 550486 MUC2 CCP58.1 Mouse IgG1 Fr, F* 550485 p16 G175-405 Mouse IgG1 Fr, F* 550834 p21 6B6 Mouse IgG1 Fr, F* 550833 p53 DO-7 Mouse IgG2b Fr, F* 550831 p53 G59-12 Mouse IgG1 Fr, F* 550832 Rb G3-245 Mouse IgG1 Fr, F* 550830 TIMP-1 102D1 Mouse IgG1 Fr, F 551007 TIMP-2 T2-101 Mouse IgG1 Fr 551119 VHL Ig32 Mouse IgG Fr 550829 Wilm s tumor (WT1) 2C12.1 Mouse IgG1 Fr, F* 550828 Mouse Bax Polyclonal Rabbit Igs Fr, F 550848 Flk-1 Avas 12α1 Rat Ig2a Fr 550549 Rat Bax Polyclonal Rabbit Igs Fr, F 550848 Crry/p65 512 Mouse IgG1 Fr 550572 Laminin B2 D18.2.2 Mouse Ig1 Fr 551251 MMP-2 staining in human breast cancer. Antibodies for Immunocytochemistry Human GM-CSF BVD2-21C11 Rat IgG2a ICC 559070 GRO 10G4.1 Mouse IgG1, k ICC 550417 IFN-γ B27 Mouse IgG1 ICC 550011 IFN-γ K3 Mouse IgG1 ICC 550521 IL-1 α 364-3B3-14 Mouse IgG1, k ICC 550004 IL-1 β AS10 Mouse IgG1, k ICC 550007 IL-2 MQ1-17H12 Rat IgG2a ICC 559067 IL-4 8D4-8 Mouse IgG1 ICC 550005 6
Antibodies for Immunocytochemistry Human (continued) IL-5 JES1-39D10 Rat IgG2a ICC 559077 IL-6 MQ2-6A3 Rat IgG2a ICC 559068 IL-8 G265-8 Mouse IgG2b ICC 550419 IL-10 JES3-12G8 Rat IgG2a ICC 559076 IL-13 JES10-5A2 Rat IgG1 ICC 559069 MCP-1 5D3-F7 Mouse IgG1 ICC 550416 MIP-1 α 11A3 Mouse IgG2a, k ICC 550418 RANTES 2D5 Mouse IgG1 ICC 550420 TNF-α MAb11 Mouse IgG1 ICC 559071 TNF-β 359-81-11 Mouse IgG1 ICC 550006 IL-4 staining in human activated PBMCs. Mouse GM-CSF MP1-22E9 Rat IgG2a ICC 559066 IFN-γ XMG1.2 Rat IgG1 ICC 559065 IL-1 α ALF-161 Hamster IgG ICC 550901 IL-2 JES6-1A12 Rat IgG2a ICC 559060 IL-3 MP2-8F8 Rat IgG1 ICC 559061 IL-4 11B11 Rat IgG1 ICC 559062 IL-10 JES5-16E3 Rat IgG2b ICC 559063 TNF-α MP6-XT22 Rat IgG1 ICC 559064 IFN-γ staining in human activated PBMCs. Isotype Controls Specificity Clone Application Cat. No. Mouse IgG1 MOPC-31C Fr, F, Zn, ICC 550878 Mouse IgG2a C1.18.4 Fr, F, Zn 550339 Mouse IgG3 J606 Fr, F, Zn 550341 Mouse IgM C48-6 Fr, F, Zn 550340 Rat IgG1, k R3-34 Fr, F, Zn, ICC 559072 Rat IgG2a, k R35-95 Fr, F, Zn, ICC 559073 Rat IgG2b A95-1 Fr, F, Zn, ICC 559478 Rat IgM R4-22 Fr, F, Zn 550342 Rat IgM R4-22 Fr, F, Zn 550343 Hamster IgG A19-3 Fr, F, Zn 550344 Hamster IgG Ha4/8 Fr, F, Zn 550345 IL-1α stain in human activated PBMCs. Second step antibodies IL-1α staining in mouse activated monocytes. anti-mouse Igs Polyclonal Goat Ig Fr, F, Zn 550337 anti-mouse IgG1 A85-1 Rat IgG1 Fr, F, Zn 550331 anti-mouse IgG2a R19-15 Rat IgG1 Fr, F, Zn 550332 anti-mouse IgG2b R12-3 Rat IgG2a Fr, F, Zn 550333 anti-mouse IgG3 R40-82 Rat IgG2a Fr, F, Zn 550334 anti-rat IgG Polyclonal Goat Ig Fr, F, Zn, ICC 559286 anti-rat IgG1/2a G28-5 Mouse Ig2a Fr, F, Zn 550325 anti-rat IgG2b G15-337 Mouse Ig2b Fr, F, Zn 550327 anti-rat IgG2c A92-1 Mouse IgG1 Fr, F, Zn 550329 anti-rat IgM G53-238 Mouse IgG1 Fr, F, Zn 550330 anti-rat, hamster Ig RG7/7.6 Mouse IgG2a Fr, F, Zn 550336 anti-hamster cocktail Mouse IgG1/2b Fr, F, Zn 550335 anti-rabbit Igs polyclonal Goat Ig Fr, F, Zn 550338 7
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