Infectious Disease Testing Urielect 4 Medium Direct Identification Visibly Reliable
Urielect 4 Non selective chromogenic medium for the isolation, differentiation and enumeration of urinary tract infections pathogens within 18-24 hours: Isolation and identification of both Gram-positive and Gram-negative pathogens in a single plate Chromogenic mix allowing the detection of specific enzymes activities Direct identification of E. coli, Enterococcus spp. and Proteus mirabilis via revelation and visualization of enzymatic activity of bacteria Presumptive identification of KEC, treptococci and taphylococci (diffentiation of. saprophyticus) Inhibition of Proteus spp. swarming with an original formulation Assay Procedure E. coli 24 hrs Proteus mirabilis 24 hrs Urine specimen inoculation with a 10 μl calibrated loop Incubation 18-24 hrs at 35-37 C Enumeration: Compare the density of colonies present on the plates, with those illustrated below: 10 6 CFU/mL 10 5 CFU/mL 10 4 CFU/mL 10 3 CFU/mL Interpretation of bacteriuria: Counting of well-isolated colonies on Urielect 4 should be possible up to 10 4 CFU/mL of urine. Above that concentration, presence of confluent colonies does not result in accurate counts.
Direct Identification of E. coli, Proteus mirabilis and Enterococcus spp. Escherichia coli Enterococcus spp. Pink colonies: β-galactosidase Indole Turquoise-blue colonies: β-glucosidase Proteus mirabilis Orange-brown colonies with possible brownish halo: TDA, Indole Presumptive Identification of KEC Klebsiella pneumoniae erratia marcescens Enterobacter cloacae Citrobacter koseri
Examples of Other Pathogens Growth taphylococcus saprophyticus taphylococcus aureus taphylococcus epidermidis treptococcus agalactiae Pseudomonas aeruginosa Candida albicans Acinetobacter baumanii
Pathogens Identification Observation of the color of colonies: Pink colonies = β-galactosidase Turquoise blue colonies = β-glucosidase Orange-brown colonies with a possible brownish halo = TDA ** Blue-purple colonies = β-galactosidase and β-glucosidase White, other color or colorless colonies Test for indole directly on a colony E.coli Examine under microscope Gram Cocci Enterococci Test for indole directly on a colony Proteus mirabilis Proteus spp., Morganella spp., Providencia spp. Examine under microscope Gram Bacilli very likely KEC * * Klebsiella, Enterobacter, erratia and Citrobacter ** Tryptophan Desaminase Complete the identification using a conventional procedure Visualization and Identification of Mixed Cultures Contrasting colors of colonies enhanced by a pearl-grey opaque background allow for an optimal visualization of polymicrobial mixtures. 18 hrs mixed culture of Klebsiella spp. and Enterococcus spp. 18 hrs mixed culture of E. coli, Enterococcus spp. and Proteus spp.
Ordering Information Catalog # Description Urinary Tract Infections 63726 Urielect 4, 20 plates x 90 mm 63727 Urielect 4, 100 plates x 90 mm 64694 Urielect 4 (dehydrated), 500 g 55313 Kovacs reagent, 2 vials x 15 ml 63713 Urea Indole medium, 50 ampules x 1 ml 53834 O.X. (Oxidase), 2 x 50 disks 56350 Novobiocin (5 µg), 30 disks 56356 Pastorex taph Plus, 50 tests 56353 Pastorex taph Plus, 5 x 50 tests 61721 Pastorex trep, 60 tests 56513 AuxaColor 2 kit, 20 tests Visit www.bio-rad.com/select-chromogenicmedia for more information. Catalog # Description Rapid Detection of Resistance to Third Generation Cephalosporins for Enterobacteriaceae 68250 b LACTA Test, 50 tests Rapid Detection of Carbapenemase-Producing Enterobacteriaceae 68260 b CARBA Test, 25 tests Rapid Identification of Carbapenemases Types K-15R1 OXA-48 K-eT* K-15R2 KPC K-eT* * Manufactured by and distributed by Bio-Rad. Ask for availability in your country. Other Chromogenic Media 63757 MRAelect II, 20 plates x 90 mm 63748 Aelect, 20 plates x 90 mm 63751 VREelect, 20 plates x 90 mm 63750 trepbelect, 20 plates x 90 mm 63740 Candielect, 20 plates x 90 mm Aelect VREelect MRAelect II Urielect 4 trepbelect Candielect Chromogenic Media Range Bio-Rad Laboratories Clinical Diagnostics Group Website www.bio-rad.com/diagnostics Australia 61-2-9914-2800 Austria 43-1-877-8901 Belgium +32 (3)710-53-00 Brazil +55 (31)3689-6600 Canada 1-514-334-4372 China 86-21-61698500 Czech Republic 420-241-430-532 Denmark +45-4452-1000 Finland 358-9-804-22-00 France 33-1-47-95-60-00 Germany +49 (0)89-318-840 Greece 30-210-7774396 Hong Kong 852-2789-3300 Hungary +36-1-459-6100 India 1800-180-1224 Israel 972-3-9636050 Italy +39-02-216091 Japan 81-3-6361-7070 Korea 82-2-3473-4460 Mexico +52 (55)5488-7670 The Netherlands +31-318-540666 New Zealand 64-9-415-2280 Norway +47-23-38-41-30 Poland 48-22-3319999 Portugal 351-21-472-7700 Russia +7-495-721-1404 ingapore 65-6415-3170 outh Africa 27-11-442-85-08 pain 34-91-590-5200 weden 46-8-555-127-00 witzerland +41 (0)26-674-55-05/06 Taiwan 886-2-2578-7189 Thailand 662-651-8311 United Kingdom +44 (0)20-8328-2000 Bulletin 16364 Rev D Printed in France with soy-based inks on paper from sustainably managed forests Bio-Rad 2016/02