OIE Reference Laboratory Reports Activities Activities in 2016 This report has been submitted : 2017-01-12 10:32:31 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Foot and mouth disease Address of laboratory: No.1 Xujiaping, Yanchangpu Lanzhou, Gansu province 730046 CHINA (PEOPLES REP. OF) Tel.: +86-931 834.25.85 Fax: +86-931 834.09.77 E-mail address: hnxiangtao@hotmail.com Website: http://www.chvst.com/english_web/index.aspx Name (including Title) of Head of Laboratory (Responsible Official): Dr. Hong Yin, Director General of LVRI Name (including Title and Position) of OIE Reference Expert: Dr. Xiangtao Liu, Deputy director of LVRI; Head of the National Reference Laboratory for FMD Which of the following defines your laboratory? Check all that apply: Governmental Research Academic OIE Reference Laboratory Reports Activities, 2016 1
ToR 1: To use, promote and disseminate diagnostic methods validated according to OIE Standards 1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such as disease diagnosis, screening of animals for export, surveillance, etc.? (Not for quality control, proficiency testing or staff training) Diagnostic Test Indicated in OIE Manual (/No) Total number of test performed last year Indirect diagnostic tests Nationally Internationally LPB-ELISA(type O) yes 4200 0 LPB-ELISA(type 1) yes 2030 0 LPB-ELISA(type A) yes 3160 0 NSP-3ABC ELISA yes 4200 0 Direct diagnostic tests Nationally Internationally Virus isolation yes 17 0 Antigen typing ELISA yes 34 0 Real Time-RT-PCR yes 3860 0 VP1 sequencing yes 84 0 Real time typing RT-PCR no 34 0 ToR 2: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards. To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease. 2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE? No 3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents to OIE Member Countries? 2 OIE Reference Laboratory Reports Activities, 2016
OIE Reference Laboratory Reports Activities, 2016 3
Type of reagent available Related diagnostic test Produced/ provide Amount supplied nationally (ml, mg) Amount supplied internationally (ml, mg) No. of recipient OIE Member Countries Region of recipients Guinea pig antisera (against FMDV type O,1 and A) VNT produced 60ml 0 1 America s and Pacific Middle East FMDV infected sera ELISA produced 40ml 0 1 America s and Pacific Middle East FMDV refernece cell culture(inactivated) PCR produced 240ml 0 1 America s and Pacific Middle East FMD LBP-ELISA kit(type O,A,1) ELISA for FMDV Antibody detection produced 14238 0 1 America s and Pacific Middle East FMDV-NSP 3ABC LISA kit ELISA for FMDV NSP Antibody detection produced 2028 0 1 America s and Pacific Middle East 4 OIE Reference Laboratory Reports Activities, 2016
FMDV mutiple RT- PCR kit RT-PCR for FMDV molecular detection produced 228 0 1 America s and Pacific Middle East FMDV real time RT- PCR kit qrt-pcr for FMDV molecular detection produced 476 0 1 America s and Pacific Middle East 4. Did your laboratory produce vaccines? No 5. Did your laboratory supply vaccines to OIE Member Countries? No ToR 3: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases 6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease? 7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease? OIE Reference Laboratory Reports Activities, 2016 5
Name of the new test or diagnostic method or vaccine developed Description and References (Publication, website, etc.) FMDV 146s antigen quantitative ELISA FMDV 1 VNT using a quantitative RT-PCR Quantitative detection of the foot-and- mouth disease virus serotype O 146S antigen for vaccine production using a double-antibody sandwich ELISA and nonlinear standard curves, Xia Feng et al., PLoS ONE, Volume 11, Issue 3, March 2016 A neutralization assay for -1 FMDV using a quantitative PCR-based endpoint assessment,song et al.,heilongjiang Animal Science And veterinary Medicine, 2016, 10: 42-47 (in Chinese) Chimeric FMDV type O VLP A novel chimeric foot-and-mouth disease virus-like particles harboring serotype O VP1 protect guinea pigs against challenge,ali H. et al., Vet Microbiol.2016,183:92-6 A recombinant Adenovirus expressing Type O FMDV A Recombinant Adenovirus Expressing P12A and 3C Protein of the Type O Foot-and-Mouth Disease Virus Stimulates Systemic and Mucosal Immune Responses in Mice.Xie Xie Y. et al., Biomed Res Int. 2016:7849203 ToR 4: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries 8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? No 9. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? 6 OIE Reference Laboratory Reports Activities, 2016
Name of the OIE Member Country receiving a technical consultancy Purpose How the advice was provided CHINA (PEOPLE'S REP. OF) FMD vaccination and PVM in 2016 in China meeting, draft plan CHINA (PEOPLE'S REP. OF) FMD Active surveillance in 2016 in China training courses, meetings, draft plan CHINA (PEOPLE'S REP. OF) Adjustment of vaccination of type A for pigs, and stoping type 1 in China meetings CHINA (PEOPLE'S REP. OF) advince on SEACFMD Roadmap Email and meeting KOREA (REP. OF) Establishment of the diagnostic technology and data analysis; Establishment of the vaccine production department At the meeting and during the visit to LVRI THAILAND FMD vaccination strategy and the vaccine production technology During the visit to LVRI JAPAN FMD surveillance and vaccination strategy During the visit to LVRI ToR 5: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations 10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? OIE Reference Laboratory Reports Activities, 2016 7
Title of the study Duration Purpose of the study Partners (Institutions) OIE Member Countries involved other than your country Research and development of an attenuated edible FMD vaccine using salmonella as the vector 3 years Development of an attenuated edible FMD vaccine using salmonella as the vector Korea Atomic Energy Research Institute/Prof. Seo HoSeong KOREA (REP. OF) Engineering Foot-and- Mouth Disease vaccine with increased antigenic match and broadened coverage of antigen for the development of effective vaccine 5 years The control of Foot-and- Mouth Disease; Effective engineering Foot-and- Mouth Disease Vaccine development Pirbright Institute, UK/Don King UNITED KINGDOM Introduction and application of the related diagnosis materials from the FMDV circulating in surrounding countries 2.5 years Established multi-qrt- PCR to detect O/Pan-2 Pirbright Institute, UK/Don King UNITED KINGDOM Exchange of vaccine technology for the delivery of oral vaccines to mucosal 3 years To explore the potential of plants and OMVs for production of antigens for oral Vaccination University of East Anglia/Professor Tom Wileman UNITED KINGDOM Genetic variation in Foot-and-mouth Disease Virus A/HUBWH strain under selective pressures of antibody and its correlation with vaccine potency 2 years This project will provides a prediction about the mutation direction of wild strains under the antibody selective pressures, which has important realistic significance on the control of foot-andmouth disease and enunciating heredity and evolution of foot-andmouth disease virus. National Microbiology Laboratory-Public Health Agency of Canada/University of Manitoba/Dr Binghua Liang CANADA ToR 6: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases 11. Did your Laboratory collect epizootiological data relevant to international disease control? 12. Did your laboratory disseminate epizootiological data that had been processed and analysed? 8 OIE Reference Laboratory Reports Activities, 2016
13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: 0 no b) International conferences: 7 Experts or participants from LVRI, report the current situation of FMD in China, exchange the information with other laboratories, such as OIE/FAO FMD network meeting. c) National conferences: 15 all the natiola coferences on FMD control and prevention, vaccinee and vaccination, diagnosis technique and some of training courese, the situation of FMD in China is as the first part to be reported. d) Other: (Provide website address or link to appropriate information) 12 VOL.18 No.2-No.11 of offical veterinary bulltine, published by MoA, China. http://www.moa.gov.cn/zwllm/tzgg/gb/sygb/ ToR 7: To provide scientific and technical training for personnel from OIE Member Countries To recommend the prescribed and alternative tests or vaccines as OIE Standards 14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries? a) Technical visits: 2 b) Seminars: 2 c) Hands-on training courses: 2 d) Internships (>1 month): 2 Type of technical training provided (a, b, c or d) Country of origin of the expert(s) provided with training No. participants from the corresponding country a China 2 b China 2 c China 2 d China 2 ToR 8: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned 15. Does your laboratory have a Quality Management System certified according to an International Standard? No OIE Reference Laboratory Reports Activities, 2016 9
Explain Quality Management System in adoption process or currently in place Applied to ISO17025. It is under review and inspection by the CNAS currently. 16. Is your laboratory accredited by an international accreditation body? Test for which your laboratory is accredited FMDV antigen ELISA Liquid-Phase Blocking ELISA 3ABC Antibody ELISA Real-Time FMDV RT-PCR Accreditation body ISO 9001,CNAS ISO 9001,CNAS ISO 9001,CNAS ISO 9001,CNAS 17. Does your laboratory maintain a biorisk management system for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Chapter 1.1.4) ToR 9: To organise and participate in scientific meetings on behalf of the OIE 18. Did your laboratory organise scientific meetings on behalf of the OIE? No 19. Did your laboratory participate in scientific meetings on behalf of the OIE? 10 OIE Reference Laboratory Reports Activities, 2016
Title of event Date (mm/yy) Location Role (speaker, presenting poster, short communications) Title of the work presented 22nd Meeting of the OIE Sub-Commission for SEACFMD 03/16 Chiang Rai, Thailand speaker Regional FMD Reference Laboratory Activities at RRL Lanzhou Coordination Meeting on TADs control in East 03/16 Shanghai, China observer FMD control roadmap in East 19th SEACFMD National Coordinators Meeting 08/16 Bangkok, Thailand presenting poster Current Laboratory Activities in China in relation to FMD 2016 International Workshop on Veterinary Epidemiology 08/16 Republic of Korea Speaker The Current FMD Situation in China The Sixth Meeting on Cross Border Trade and Transboundary Animal Diseases (TADs) Risk Reduction between China, Mongolia and Russia 10/16 Vladimir, Russia Speaker FMD Epidemiology and Control in China The 2016 Open Session of the Standing Technical Committee of the an Commission for the Control of Foot-and- Mouth Disease 10/16 Cascais, Portugal Speaker The Current Epidemiology and Control of FMD in China The 2016 OIE-FAO FMD Laboratory Network Meeting 11-12/16 Paris,France Speaker The current FMD situation in China The sixth meeting on control of FMD and AIV in East 12/16 Beijing,China Speaker FMD Epidemiology and Control in China Project Formulation Workshop on FAO-China South-South Cooperation Project on Transboundary Animal Disease Control in Greater Mekong Sub- Region 12/16 Bangkok,Thailand Speaker FMD Epidemiology and Control in China OIE Reference Laboratory Reports Activities, 2016 11
ToR 10: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results 20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease? 21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests? Purpose of the proficiency tests: 1 Role of your Reference Laboratory (organiser/ participant) No. participants Participating OIE Ref. Labs/ organising OIE Ref. Lab. Harmonisation of testing between national reference laboratories participant 1 The Chinese national/oie reference laboratory for FMD 1 validation of a diagnostic protocol: specify the test; quality control of vaccines: specify the vaccine type, etc. 22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest? Title of the project or contract Scope Name(s) of relevant OIE Reference Laboratories SEACFMD FMD control and prevention in SEA nationas FMD regional reference lab,pachong,thailand Introduction and application of the related diagnosis materials from the FMDV circulating in surrounding countries Established multi-qrt-pcr to detect O/Pan-2 A/Iran/05 and A//G-VII and other virus strains The World/OIE reference laboratory for FMD, The Pirbright Institute, UK Engineering Foot-and-Mouth Disease Vaccine with Increased Antigenic Match and Broadened Coverage of Antigen for the Development of effective Vaccine The control of Foot-and-Mouth Disease; Effective engineering Footand-Mouth Disease Vaccine development The World/OIE reference laboratory for FMD, The Pirbright Institute, UK 12 OIE Reference Laboratory Reports Activities, 2016
ToR 11: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results 23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease? Note: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: http://www.oie.int/en/our-scientific-expertise/reference-laboratories/proficiency-testing see point 1.3 Purpose for inter-laboratory test comparisons 1 No. participating laboratories Region(s) of participating OIE Member Countries FMD antigen detection by real-time RT-PCR in 31 provinces level veterinary laboratories, China 31 Americas and Pacific Middle East FMD PT on antiboday and antigen detection in 5 provinces level veterinary laboratories, China 5 Americas and Pacific Middle East ToR 12: To place expert consultants at the disposal of the OIE 24. Did your laboratory place expert consultants at the disposal of the OIE? OIE Reference Laboratory Reports Activities, 2016 13
Kind of consultancy Location Subject (facultative) OIE asks for comment from FMD experts LVRI Make suggestions for revision of FMD diagnosis OIE asks for comment from FMD experts Beijing,China Animal Health Code revision WRL(Pribright laboratory)asks for comment from Dr. Yanmin Li, FMD expert in LVRI LVRI, Lanzhou; ANSES, Paris FMDV VNT and r valure test Dr Yanmin Li provided comments and suggestions for the OIE terrestrial animals code and mannual Beijing, China Annex 19 Chapter 15 (PRRS); Annex 22 Chapter 4 (vaccination) Dr Zhidong Zhang provided comments and suggestions for the OIE terrestrial animals code and mannual Beijing, China Annex26 Article 7.1.X Guiding Principles on the Use of Animal based measures;annex18_chapter_15_1_asf;annex16_chapter_11_lsdv Dr Yanmin Li provided suggestion and consulancy on the control measures for the illegal movemnet of animals and the FMD spreading in the border area between Myanmar and China Bangkok, Thailand Cooperation project on transboundary animal disease control in Greater Mekong Sub-Region 25. Additional comments regarding your report: no 14 OIE Reference Laboratory Reports Activities, 2016