Laboratory Activities Health Sciences 1110 Module 5-Microbiology Watch Raw Terror: E. coli Bacteria video o Answer the questions on your laboratory worksheet. View the images listed below in WebPath and answer the questions on your worksheet. o Click on the WebPath link on the Lab 5 lab page. o Click on the General Pathology link o Click on the Infection link o Click on the title of the graphic number to view the images. Answer the questions on your worksheet as appropriate. Graphic 15 Oral candidiasis, gross Graphic 16 Oral candidiasis, PAS stain, microscopic Answer question #1 on your worksheet. Graphic 17 Aspergillus fungal abscess, lung, gross Graphic 20 Aspergillus fungus ball, lung, high power microscopic Answer question #2 on your worksheet. Graphic 28 Herpes simplex virus, skin, high power microscopic Answer question #3 on your worksheet. Graphic 36 Mycobacterium tuberculosis, lung, AFB stain, microscopic Answer question #4 on your worksheet. Graphic 43 Ascaris lumbricoides, gross Answer question #5 on your worksheet. Graphic 47 Sarcoptes scabiei (scabies mite), microscopic Answer question #6 on your worksheet. Graphic 48 Head lice, gross Graphic 49 Lice, microscopic Answer question #7 on your worksheet
o Click on Return to the WebPath General Pathology Menu o Click on Return to the Webpath Main Menu o Click on the Clinical Pathology link o Scroll down to the microbiology section o Click on the title of the graphic number to view the images. Answer the questions on your worksheet as appropriate. Graphic 2 Trichuriasis, colon, gross Graphic 4 Giardiasis, small intestine, microscopic Answer question #8 on your worksheet. Graphic 22 Mycobacterium leprae, granuloma in skin, Fite's acid fast stain, microscopic Answer question #9 on your worksheet. Graphic 43 Neisseria gonorrheae, gram stain Answer question #10 on your worksheet. Graphic 28 Malaria (Plasmodium vivax) in peripheral blood smear, microscopic Answer question #11 on your worksheet Graphic 30 Trachoma, conjunctival scraping, giemsa stain, high power microscopic Go through the CyberEd Online activities by clicking on the CyberEd Online link on the Lab 5 lab page o To access the CyberEd activities, use the username and password found below. Username: weberstate Password: science o Click on the biology icon or the grasshopper o Click on the appropriate connection speed icon (DSL+ high speed or 56k dial up ) for: o Note: Campus students click high speed connection Viruses & Bacteria o Answer the post-test questions for each section; they could appear on the Module 5 Lab Quiz
Module 5-Microbiology Laboratory Worksheet Answer the following questions as you watch the Raw Terror: E. coli Bacteria video 1. List one of Damion s first symptoms. 2. What is happening to his red blood cells? 3. What was the first organ affected by his disease? 4. What does HUS stand for? 5. Damion s system is overflowing with. 6. What kept Damion alive? 7. Name the procedure that would wash out the toxins in Damion s blood. 8. 96-98% of the cases of HUS are caused by. 9. What was discovered when Damion drank an Icee? 10. How did Damion get infected with E. coli? 11. How long was he in intensive care? 12. How many surgeries did he have? 13. In 1993, how many individuals were killed from E. coli from Jack In the Box? 14. T or F: E. Coli is the leading cause of kidney failure in children. 15. What percent of lung capacity has Damion lost?
Webpath Questions 1. Candidiasis involves infestation by yeastlike fungi of the genus Candida. Candidiasis (the oral form is called thrush) is common in patients who are diabetic, who are taking long-term antibiotics, or in patients who a. have undergone surgery b. are immunocompromised (weakened immune system). c. have been exposed to radiation. d. have undergone oral intubation. 2. Aspergillus is a genus of mold-like fungi. One of its characteristic growth features is the presence of a. tubercles. b. lesions. c. chancres. d. hyphae. 3. Herpes simplex most often infects a. the liver. b. the lungs. c. connective tissue. d. skin and mucous membranes. 4. A red, acid-fast (a stain that is not washed away by acid) has been used in the photomicrograph. This type of stain, when applied to lung tissue infected by Mycobacterium tuberculosis, results in a. a three-dimensional effect. b. red-stained cell and blue bacteria. c. red-stained bacteria (red rods). d. none of the above. 5. Ascaris lumbricoides is an example of a pathogenic a. virus. b. worm. c. bacterium. d. protozoon.
6. Infestation by scabies mites often results in lesions which are excoriated. Excoriation, in this case, refers to a. abrasions of the epidermis caused by scratching. b. ulcer formation and bleeding caused by toxins. c. hardening of the skin, also called scleroderma. d. formation of a sac containing liquid, also called vesiculation. 7. Head lice are easily visible to the naked eye, appearing about the size of sesame seeds in the hair. Lice are a. insects b. spiders c. worms d. protozoans 8. Hikers who drink from streams may ingest the protozoon Giardia lamblia, which causes backpackers diarrhea (giardiasis). According to the photomicrograph, this condition may be confirmed by a. serological tests. b. microscopic examination of a saliva sample. c. microscopic examination of fecal material (stool sample). d. urinalysis. 9. Leprosy is caused by Mycobacterium leprae, which is visible (with difficulty) in the photomicrograph. These bacteria are seen within a spherical aggregation of macrophages and other cells that are often associated with inflammatory reactions. The general term for this kind of spherical aggregation is a a. vesicle. b. lesion. c. cyst. d. granuloma.
10. Neisseria is a genus of gram negative diplococcal bacteria. Specific species of Neisseria commonly cause a. the flu or cold. b. malignancies of lymphomas. c. gonorrhea or meningitis. d. pneumonia or pharyngitis. 11. The pathogen Plasmodium vivax is a protozoon that causes malaria. In the photomicrograph above, it is visible inside the patient s a. neutrophils. b. RBCs c. lymphocytes d. WBCs Graphics for Module 5 Exam See attached graphics.
Answers for video questions 1. diarrhea; fever 2. They were being destroyed 3. intestines 4. hemolytic uremic syndrome 5. toxins 6. dialysis 7. plasma exchange 8. E. coli 0157 9. hole in his intestine 10. he ate raw meat 11. 5 weeks 12. 7 13. 3 14. True 15. 25% Answers for WebPath questions 1. B 6. A 11. B 2. D 7. A 3. D 8. C 4. C 9. D 5. B 10. C
Figure 1 Virus
Figure 2 General Bacterium