Bone Marrow Specimen (Aspirate and Trephine Biopsy) Proforma Mandatory questions (i.e. protocol standards) are in bold (e.g. S1.03). Family name Given name(s) Sex Male Female Intersex/indeterminate Date of birth DD MM YYYY Ethnicity Unknown Aboriginal/Torres Strait Islander Other ethnicity: Patient identifiers e.g. MRN, IHI or NHI (please indicate which) Date of request S1.03 Accession number Requesting doctor - name and contact details DD MM YYYY Clinical details Indication G1.01 Other clinical information received Record of procedure S2.01 Date of the BM specimen collection Clinical history S2.02 BM Biopsy performed by Not known Relevant results S2.03 Anatomical site of the aspirate/biopsy (include side) Test requested G2.01 Ease/difficulty of the aspiration S1.04 Principal clinician caring for the patient and contact details G2.02 Sedation V0.1 Proforma: Bone Marrow Specimen (Aspirate and Trephine Biopsy) Structured Reporting Protocol 1st Ed. Page 1 of 8
S2.04 Specimen type(s) Peripheral blood smear Aspirate - smear Aspirate squash prep Trephine biopsy Trephine imprint S2.05 Distribution of biopsy material/ additional tests requested Flow cytometry Immunohistochemistry Cytogenetics FISH Molecular studies Microbiology Tissue banking Morphological findings - ASPIRATE S3.01 Cellularity of particles Acellular Hypocellular: Reduced with regard to the Normocellular: for the Hypercellular: Increased with regard to the Dry Tap Blood Tap Haemodilute marrow sample AND (if applicable) Scant particles for assessment Degree (If particles hypocellular/hypercellular) G2.03 Additional comments Mildly Moderately Markedly S2.06 BLOOD COUNT RESULTS Date of last FBC FBC results Hb g/l Cellularity of trails (if different to particles) Acellular Hypocellular: Reduced with regard to the Normocellular: for the Hypercellular: Increased with regard to the Dry Tap WCC X 10 9 /L Blood Tap MCV fl Haemodilute marrow sample NEU X 10 9 /L LYM X 10 9 /L MON X 10 9 /L EOS X 10 9 /L BAS X 10 9 /L PLT X 10 9 /L Blood film results AND (if applicable) Scant particles for assessment Degree (If trails hypocellular, or hypercellular) Mildly Moderately Markedly Cellularity of imprint (if required) Acellular Hypocellular: Reduced with regard to the Normocellular: for the Hypercellular: Increased with regard to the Dry Tap Blood Tap Haemodilute marrow sample V0.1 Proforma: Bone Marrow Specimen (Aspirate and Trephine Biopsy) Structured Reporting Protocol 1st Ed. Page 2 of 8
Degree (If imprint hypocellular/hypercellular) Mildly Moderately Markedly S3.02 Nucleated differential cell count Type Normoblastic Megaloblastic (nuclear-cytoplasmic asynchrony) Dyserythropoietic (describe including percentage of abnormal cells) Inadequate specimen Total number of nucleated cells counted Other (e.g. giant pronormoblast)(specify) AND List each cell type counted and a numeric value Cell type Result S3.04 GRANULOPOIESIS Cellularity Maturation Left-shifted Dysgranulopoietic Maturation arrest (stage: ) Lymphoid aggregates (if seen) Myeloid morphology (including percentage of abnormal cells) M:E Ratio / S3.03 ERYTHROPOIESIS Cellularity If maturation is not normal describe: Leucocyte subpopulations (including blasts (where relevant), mast cells - a qualitative and quantitative description) V0.1 Proforma: Bone Marrow Specimen (Aspirate and Trephine Biopsy) Structured Reporting Protocol 1st Ed. Page 3 of 8
S3.05 MEGAKARYOPOIEISIS Cellularity G3.01 Other marrow cells (if required) Histiocytes (macrophages) Osteoblasts Osteoclasts Morphological features (if required) Morphology Micromegakaryocytes Nuclear hypolobation Multinucleation (widely separated nuclei) Other eg pyknotic (specify) G3.02 Abnormal infiltrating nonhaematopoietic cells (if present) AND Abnormal megakaryocytes % Large platelet clumps G3.03 Haemophagocytosis Mild Moderate Marked Cell types ingested S3.06 LYMPHOCYTES Reduced Increased Morphology (including size and nuclear and cytoplasmic features) S3.08 Iron stores Reduced Increased AND/ S3.07 PLASMA CELLS Increased 0 1 2 3 4 5 6 Sideroblasts (if relevant) Morphology (including size and nuclear and cytoplasmic features) % Morphology (eg coarse/ringed) V0.1 Proforma: Bone Marrow Specimen (Aspirate and Trephine Biopsy) Structured Reporting Protocol 1st Ed. Page 4 of 8
S3.09 Other significant features of the aspirate (if required) Degree (Optional) Mildly Moderately Markedly S3.10 Other comment on the aspirate (if required) AND/ Architecture % Abnormal S3.11 Reporting haematologist / pathologist (aspirate) (include designation) Interstitial infiltrate Focal infiltrate Paratrabecular Nonparatrabecular Date Nodular infiltrate Diffuse infiltrate Distorted due to fibrosis Morphological findings - TREPHINE S3.12 Trephine biopsy identifier S3.13 MACROSCOPIC Areas of abnormality (if present) (e.g. necrosis, amorphous eosinophilic material, serous atrophy, haemorrhage or fibrosis) of cores Aggregate length Adequacy Adequate for examination Inadequate mm Artefactual changes Description (if required) S3.14 Trabecular bone architecture S3.15 Cellularity Acellular Hypocellular: Reduced with regard to the Normocellular: for the Hypercellular: Increased with regard to the S3.16 ERYTHROPOIESIS Maturation V0.1 Proforma: Bone Marrow Specimen (Aspirate and Trephine Biopsy) Structured Reporting Protocol 1st Ed. Page 5 of 8
Left shifted AND if not normal, record Morphological abnormalities S3.18 MEGAKARYOPOIEISIS Morphology S3.17 GRANULOPOIESIS Cellularity Maturation Left-shifted Maturation arrest Preponderance of immature cells Distribution in the marrow (if required eg clusters) S3.19 LYMPHOCYTES (if required) Reduced Increased If increased record Distribution Paratrabecular Interstitial Nodular Diffuse Size and number lymphoid aggregates (if required) AND if not normal, record Morphological abnormalities (if required) Morphology (eg size, N:C ratio etc) Abnormal localisation of immature precursors (if required) S3.20 PLASMA CELLS % of nucleated cells AND if abnormal record, Morphology (eg immaturity, inclusions) V0.1 Proforma: Bone Marrow Specimen (Aspirate and Trephine Biopsy) Structured Reporting Protocol 1st Ed. Page 6 of 8
AND Distribution Interstitial Microaggregate (10-50 cells) Nodular (>50 cells) Diffuse S3.22 Reporting haematologist / pathologist (trephine) (include designation) Date S3.21 Other cells (if required) Additional study findings G4.01 IMMUNOHISTOCHEMISTRY Nonhaematopoietic infiltration (if required) FLOW CYTOMETRY Granuloma formation (if required) CYTOGENETICS G3.04 RETICULIN Grade For myeloproliferative neoplasms use: else use: Pattern Diffuse 0 1 2 3 Focus MOLECULAR STUDIES FISH MICROBIOLOGY (including staining cultures and PCR) G3.05 Other comment on the trephine biopsy Additional test result G3.06 (Immuno)histochemical stains V0.1 Proforma: Bone Marrow Specimen (Aspirate and Trephine Biopsy) Structured Reporting Protocol 1st Ed. Page 7 of 8
Synthesis and overview S5.01 WHO disease subtype G5.01 Conclusion S5.02 Reporting haematologist / pathologist (Integrated report) V0.1 Proforma: Bone Marrow Specimen (Aspirate and Trephine Biopsy) Structured Reporting Protocol 1st Ed. Page 8 of 8