Channel Catfish Production in 4-m 3 LVHD Cages with a Soy-Based Feed, Jiangxi Province, China

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Key Words: Channel catfish, soybean meal, LVHD cage technology, China Channel Catfish Production in 4-m 3 LVHD Cages with a Soy-Based Feed, Jiangxi Province, China Michael C. Cremer, Zhou Enhua and Zhang Jian ASA-IM/China Aquaculture Program INTRODUCTION A feeding trial was jointly conducted by the American Soybean Association International Marketing (ASA-IM) program and the Jiangxi Provincial Fishery Extension Center to compare channel catfish production in Low Volume High Density (LVHD) cages with an ASA-IM soy-based feed versus production with a locally manufactured feed. The feeding trial was conducted at the Wanan County Fishery Bureau cage fish farm in Wanan Reservoir, Jiangxi Province, China. FEEDING TRIAL PROTOCOLS Six, 4-m 3 LVHD cages were used in the feeding trial to demonstrate the productivity and economic return of channel catfish cage culture using the ASA-IM cage technology. Locally produced channel catfish fingerlings were stocked in the six LVHD cages at a density of 225 fish per m 3 of cage volume, or 900 fish per cage. Mean weight of the catfish in all cages at stocking was 55 g. Catfish were stocked in the trial cages on 30 April 2006 and fed for 176 days. The target harvest size for channel catfish was 750 g per fish, representing a fish biomass at harvest of 150 kg/m 3 of cage volume, or 600 kg per cage. Catfish in three of the trial cages were fed the ASA-IM 32/6 growout feed (Tables 1-3). The ASA-IM 32/6 feed is an all or primarily plant protein feed that derives the majority of protein from soybean meal 1. Fish in the three cages were fed to satiation twice daily, with the fish in all cages receiving the same amount of feed at each feeding. All feed was fed in extruded, floating pellet form. 1 The numerical component of the feed description refers to the percentage of protein and fat, respectively, in the ration, i.e. 32/6 indicates 32% crude protein and 6% crude fat.

Catfish in the other three LVHD cages were fed a locally produced feed having the same 32% crude protein level as the ASA-IM feed. Fish were fed the local feed twice daily, following the same protocols as the cages receiving the ASA-IM feed. The trial was conducted over a 6-month period. Data on fish survival, gross and net production, average fish weight, and feed conversion efficiency were obtained at harvest for all cages. All fish from each cage were counted and weighed at harvest to obtain this data. Data on production input costs was recorded throughout the trial to determine the economic return of the ASA-IM and local feeds. FEEDING TRIAL RESULTS Channel catfish fed the ASA-IM 32/6 soy-based feed performed better than catfish fed the local 32% protein feed, in terms of fish growth, feed conversion efficiency and economic return. Channel catfish fed the ASA-IM 32/6 feed grew from 55 g to an average weight of 737 g in 176 days of feeding (Table 4). Catfish in each of the three ASA-IM cages were fed a total of 741.2 kg of feed, which yielded an average of 504 kg of net fish growth per cage with an average FCR of 1.47:1. In comparison, the channel catfish fed the local feed grew to 691 g with an FCR of 1.61:1. Fish growth on the local feed was 6.8% less than on the ASA-IM feed, and FCR with the local feed was 9.5% higher than FCR with the ASA-IM feed. Catfish production in the ASA-IM cages averaged 553.2 kg per cage (138.3 kg/m 3 of cage volume), and was 9% higher than catfish production with the local feed. Catfish survival averaged 83.5% in the ASA-IM cages and 81.3% in the local feed cages (Table 4). Fish health and survival were compromised during a period of high water turbidity from March to June. Catfish became diseased during this high turbidity period and approximately 15% of the ASA-IM cage fish and 18% of the local feed cage fish died. Net economic return for the ASA-IM cages averaged RMB 2,172 ($275) per cage, and yielded a return on investment (ROI) of 58.8%. Net economic return was 34% higher, and ROI 15.8% higher, with the ASA-IM feed than with the local feed (Table 4). SUMMARY AND CONCLUSIONS Channel catfish grew rapidly and efficiently on the ASA-IM 32/6 soy-based feed. Average channel catfish growth in the 4-m 3 LVHD cages was 3.88 g per day for the 176- day trial period, and the ASA-IM feed yielded an acceptable FCR of 1.5:1. Better fish growth, lower FCR and higher economic return indicated the soy-based ASA-IM feed was superior to the local feed with the same 32% crude protein level. ACKNOWLEDGEMENTS ASA-IM gratefully acknowledges the participation and cooperation of the Jiangxi Provincial Fishery Extension Center and the Wanan County Fishery Bureau. These organizations contributed significant time, personnel and facilities to conduct and supervise the channel catfish LVHD cage study detailed in this report.

Table 1. Formula for the ASA-IM 32/6 soy-based feed used in the 2006 channel catfish LVHD cage feeding trial in Wanan Reservoir, Jiangxi Province, China. The feed was fed in extruded, floating pellet form. The feed was produced as a least-cost formulation by Techbank Feed Mill, Shanghai, under supervision of ASA-IM. Individual batches of feed produced over the trial duration may have varied in ingredient composition due to differences in specific ingredient nutrient profiles and ingredient availability. Ingredient Percent of total Soybean Meal 46% 51.00 Wheat Middlings 14% 14.00 Wheat Flour 11% 12.00 DDGS, 27/10 11.50 Fish Meal, 65/8 3.00 Ca Phosphate Mono 21% 2.00 Soy Oil 1.50 Soy Lecithin 1.50 Corn Gluten Meal 60% 1.50 Fish Oil 1.20 Vit PMX F-2 0.50 Min PMX F-1 0.25 Stay C 35% 0.03 Ethoxyquin, SQ mixture 6 0.02 TOTAL 100.00

Table 2. Calculated nutritional profile of the ASA-IM 32/6 soy-based feed used in the 2006 tilapia LVHD cage feeding trial in Wanan Reservoir, Jiangxi Province, China. The feed was produced in extruded, floating pellet form. Nutrient Value, As Fed DE Fish (extruded) 2369.68 NFE 39.83 Starch 17.89 Protein, crude 32.74 Protein, digestible 29.82 Fish Protein 1.95 Soy Protein 23.46 Fat 6.07 W-3 (omega 3 fatty acid) 0.57 W-6 (omega 6 fatty acid) 2.08 Ash 6.06 Calcium 0.60 Phosphorus, available 0.61 Choline 2469.93 Vitamin C 105.00 Ethoxyquin 134.50 Arginine 2.06 Isoleucine 1.63 Lysine 1.85 Methionine 0.50 Methionine + Cystine 1.00

Table 3. Vitamin and mineral premix formulations included in the ASA-IM 32/6 soybased feed used in the 2006 tilapia LVHD cage feeding trial in Wanan Reservoir, Jiangxi Province, China. Quantities of vitamins and minerals are per kilogram of premix. Ingredient Unit Amount Vitamin Premix F-2 Vitamin A IU/kg 1,200,000 Vitamin D3 IU/kg 200,000 Vitamin E IU/kg 20,000 Vitamin K mg/kg 0 Vitamin C mg/kg 0 Biotin mg/kg 40 Choline mg/kg 0 Folic Acid mg/kg 1,800 Inositol mg/kg 0 Niacin mg/kg 40,000 Pantothenate mg/kg 20,000 Pyridoxine (B6) mg/kg 5,000 Riboflavin (B2) mg/kg 8,000 Thiamin (B1) mg/kg 8,000 Vitamin B12 mcg/kg 2,000 Ethoxyquin mg/kg 500 Mineral Premix F-1 Iron ppm 40,000 Manganese ppm 10,000 Copper ppm 4,000 Zinc ppm 40,000 Iodine ppm 1,800 Cobalt ppm 20 Selenium ppm 200

Table 4. Results of the 2006 ASA-IM aquaculture trial in Wanan Reservoir, Jiangxi Province that compared fingerling to market growth performance of channel catfish in 4-m 3 LVHD cages when fed either the ASA-IM 32/6 soy-based feed or a local feed containing 32% crude protein. Cage Cage Stocking Stocking rate No. days Harvest 1 P G Survival FCR Net income ROI No. size size (g) (fish/m 3 ) fed wt (g/fish) (kg/m 3 ) (%) feed only (RMB/m 3 ) 2 (%) 1 4-m 3 55 225 176 722 137.7 84.8 1.48 537 58.2 2 4-m 3 55 225 176 740 136.1 81.8 1.50 520 56.3 3 4-m 3 55 225 176 748 141.0 83.8 1.44 572 62.0 Mean 55 225 176 737 138.3 83.5 1.47 543 58.8 4 4-m 3 55 225 176 672 122.7 81.2 1.67 360 38.2 5 4-m 3 55 225 176 700 127.7 81.0 1.60 412 43.8 6 4-m 3 55 225 176 702 130.5 81.6 1.57 441 46.9 Mean 55 225 176 691 127.0 81.3 1.61 405 43.0 1 P G = Gross Production 2 RMB exchange rate: RMB 7.9 = $1.00