Moisturization + Antioxidant + Hair Nourishing Tomorrow s Vision Today!
Technical Information: Product Code: 20039 INCI Name: Tapioca Starch and Lactobacillus Ferment Lysate INCI Status: Conforms Suggested Use Level: 1.0 10.0% Suggested Applications: Moisturizing, Wound Healing, Antioxidant
Tapioca Characteristics & History: Starch derived from South American cassava plant Distinct composition of carbohydrates, vitamins, minerals, and organic compounds o Rich in fiber, protein, and good cholesterol o Low levels of saturated fat, sodium, and bad cholesterol Commonly used in the nutritional industry as a glutenfree thickening agent Enjoyed throughout the world as the main constituent of bubble tea
Tapioca Characteristics & History: Cassava has traditionally been used as an herbal remedy for skin sores Today, tapioca starch is a vital tool used in developing countries to fight dehydration Cassava derived sugars contain an active carbohydrate profile that offers a wide range of cosmetic benefits o Traditional sugar based materials are used as exfoliators
Development: ACB Botanical Sugar Complex harnesses the benefits of tapioca starch through the fermentation of cassava root with Lactobacillus Fermentation allows for the isolation of the tapioca starch and enhanced bioavailability of the active o Increases skin and hair benefits by enhancing the ability of the constituents to be more readily absorbed
Benefits: Increases Cellular Metabolism Intense Moisturization Antioxidant Hair Nourishing Enhanced Bioavailability Wound Healing Restores Moisture Balance Increases Cellular Viability
Efficacy Data In-vitro Scratch Assay Scratch Area (mm 2 ) 1 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0 T = 0 T = 24 T = 48 T = 72 Time Post Scratch 100ng/mL EGF Complete Media SFM ACB Botanical Sugar Complex Figure 1. Area of Scratch Protocol Human dermal fibroblasts were seeded into 6-well tissue culture plates 0.01% concentration of ACB Botanical Sugar Complex was added to the serumfree DMEM and incubated with fibroblasts Scratch took place every 24 hours and up to 96 hours ACB Botanical Sugar Complex was able to increase cell migration and close the scratch at a rate comparable to the positive control Product has healing abilities and cell proliferation properties
Efficacy Data In-vitro Scratch Assay Percent (%) Closure 100 90 80 70 60 50 40 30 20 10 0 T = 24 T = 48 T = 72 Time Post Scratch 100ng/mL EGF Complete Media SFM ACB Botanical Sugar Complex Protocol Human dermal fibroblasts were seeded into 6-well tissue culture plates 0.01% concentration of ACB Botanical Sugar Complex was added to the serum-free DMEM and incubated with fibroblasts Scratch took place every 24 hours and up to 96 hours ACB Botanical Sugar Complex was able to increase cell migration and close the scratch at a rate comparable to the positive control Product has healing abilities and cell proliferation properties Figure 2. Percent Scratch Closure
Efficacy Data In-vitro Scratch Assay Migration (µm/24 hours) 25 20 15 10 5 0 T = 24 T = 48 T = 72 Time Post Scratch 100ng/mL EGF Complete Media SFM ACB Botanical Sugar Complex Protocol Human dermal fibroblasts were seeded into 6-well tissue culture plates 0.01% concentration of ACB Botanical Sugar Complex was added to the serum-free DMEM and incubated with fibroblasts Scratch took place every 24 hours and up to 96 hours ACB Botanical Sugar Complex was able to increase cell migration and close the scratch at a rate comparable to the positive control Product has healing abilities and cell proliferation properties Figure 3. Cell Migration Rate
Efficacy Data Protocol Human dermal fibroblasts were seeded into 6-well tissue culture plates 0.01% concentration of ACB Botanical Sugar Complex was added to the serumfree DMEM and incubated with fibroblasts Scratch took place every 24 hours and up to 96 hours ACB Botanical Sugar Complex was able to increase cell migration and close the scratch at a rate comparable to the positive control Product has healing abilities and cell proliferation properties Figure 4. Images at t=0 hours (A, D, G) and t=72 hours (B, E, H) for ACB Botanical Sugar Complex, positive control (EGF-1), and negative control (SFM). At experiment completion (t=72 hours), cells were fixed in paraformaldehyde and stained with crystal violet (C, F, I).
Efficacy Data Cellular Viability Assay Relative Fluorescence Units (RFU) 94000 92000 90000 88000 86000 84000 82000 80000 78000 Protocol Human dermal fibroblasts seeded into 96- well tissue culture plates Concentrations: 0.01%, 0.1%, 1.0% 10 µl of viability reagent was added to 90 µl of cell culture media in culture wells ACB Botanical Sugar Complex exhibited comparable results by increasing cell metabolism 76000 74000 Complete Media 1% ACB Botanical Sugar Complex 0.1% ACB Botanical Sugar Complex 0.01% ACB Botanical Sugar Complex Figure 5. Increase in Cell Renewal following application of ABS Botanical Sugar Complex
Efficacy Data ORAC Assay 250 Antioxidant Capacity (μmte) 200 150 100 50 Protocol Trolox was used as the positive control Test Quantity: 5.0%, 2.5%, 1.25% Fluorescent measurements were taken every two minutes for two hours Antioxidant capacity of ACB Botanical Sugar Complex increased as concentration increased 0 200μM Trolox 12.5μM Trolox 5.0% ACB Botanical Sugar Complex 2.5% ACB Botanical Sugar Complex 1.25% ACB Botanical Sugar Complex Figure 6. Antioxidant Capacities of ABS Botanical Sugar Complex
Technical Information: Product Code: 20039 INCI Name: Tapioca Starch and Lactobacillus Ferment Lysate INCI Status: Conforms Suggested Use Level: 1.0 10.0% Suggested Applications: Moisturizing, Wound Healing, Antioxidant
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