Eurofins Carbohydrate Competence Centre: Your partner for sweet analyses Dr. Jeroen van Soest BU manager Dr. Yannis Vrasidas Scientific support manager Santiago, Chili, 2017 www.eurofins.com
Carbohydrate Competence Centre Expertise BU Manager: Dr. Jeroen van Soest specialist in carbohydrates & food-feed-pharma-non-food products PhD on starch bioplastics > 100 publications/patents/standards in the field winner several prizes e.g. 2 cereal prizes, bioplastic Oskar. CCC lead scientist & co-author: Dr. Yannis Vrasidas carbohydrates and modification prebiotics & dietary fibres & HMOs pharma and food
Outline Eurofins Group & Carbohydrate Competence Centre Topic 1 9 Tuesday 9:20-9:40 (SESIÓN PARALELA 5 SALÓN CORDILLERA) Reemplazo de ingredientes críticos por fibra dietaria. Cómo la interacción de ingredientes incide en las mediciones y dificultades observadas. = Replacement of critical ingredients with dietary fiber. How the ingredients affects the measurements and difficulties observed. Topic 2 Tuesday 14:50-15:10 (SESIÓN PARALELA 9 SALÓN CORDILLERA) Un ejemplo: el desarrollo del método de Fibra Dietaria relacionada a alimentos funcionales con ingredientes destinados a reducir nutrientes. Polialcoholes, inulinas, edulcorantes u otros. Apoyo en la legislación Unión Europea. = An example: Dietary Fiber methods related to functional foods with nutrient reducing ingredients, e.g. polyalcohols, inulins, sweeteners or others. Consequences for (European Union) legislation.
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Carbohydrate Competence Centre History & Facts 34 employees, 28 FTE (analysts & scientists) with strong expertise in - Carbohydrate, Food, Analytical Chemistry & Analysis Wide portfolio of routine & specialty analyses - > 80 tests - 4000 analyses / month - based on (inter)national standard methods (AOAC, ISO, AACC) - in-house methods / national and international clients Development of new methods Member or active in international leading commissions - AOAC / SPIFAN recently 1 st action standard AOAC (Inulin-Fructan) method with Nestle - ISO / IDF / NEN developing new sugar profile with ISO-IDF & industrial partners Strong collaboration with universities & technical colleges
Portfolio Main Segments / Markets Segments / Markets Food general Feed Ingredients Dairy (milk, yoghurt, ice cream,..) Infant formula / Baby food Bakery (bread, biscuits, ) & Pasta Beverages (beer, energy drinks, juice, coffee-tea,..) Carbohydrates All kind of carbohydrates, labeling sugars, reducing & digestible carbohydrates, sucralose/alditols Gelatinized starch, galactomannan, hydrocolloids, raff/stach/verb Inulin/FOS, GOS, b-glucans, starch, pectins Lactose (free), hydrocolloids, label & specialty sugars Inulin/FOS, GOS, sialic acid, dietary fibre, sugars incl. galactose, palatinose, lactulose, polydextrose, inositol Starch, resistant starch, dietary fibre, malto-oligosaccharides, betaglucans, pectins, amylose/amylopectin, b-glucans, sweeteners, hydrocolloids, coffee sugars
DF Topic 1 Replacement of critical ingredients with dietary fiber. How the ingredients affects the measurements and difficulties observed. Dietary fibres: What? Why? How? see our Folder on DF 9:20-9:40
Fibre components
What? Markets (examples) Ingredients Final products Retail Products (examples) Bakery Pasta Beverages Dairy Infant & Baby food Healthy food products
What? Components / sources DIETARY FIBRE (DF) High Molecular Weight (HMWDF) Resistant starch (RS) Low Molecular Weight (LMWDF and prebiotic) Insoluble HMW (IHMWDF) - Cellulose - Lignin - Hemicellulose (insoluble pentosans) - Insoluble pectin - b-glucans (yeast/moulds) -... Soluble HMW (SHMWDF) - Hydrocolloids - carrageenan - gums -... - (Soluble) pentosans - Soluble pectins - Cereal b-glucan -... - RS 1 Physical inaccessible starch - RS 2 Resistant starch granules - RS 3 Retrograded starch - RS4 Chemically modified starch - Inulin - Fructose oligosaccharide (FOS) - Galactose oligosaccharide (GOS) - Polydextrose - Resistant maltodextrin -...
Dietary Fibre Analysis
Why do we need (to analyze) DF? Health benefits Blood sugar control; Diabetes Cholesterol control Weight loss & management Reduce risk of heart disease Prebiotic activity Dietary fibre content on product label The more the better
Complications by different investigations Different analytical methods applied for quantifying the amount of dietary fibre Possible double estimation of dietary fibre components if different methods combined Country-to-country differences in the dietary fibre composition e.g. South vs Northern European diets regarding the physiological effects Country-to-country differences in legislation UK recommended 18 g/day DF intake (as NSP by the Englyst method) Other countries 25-35 g/day DF intake (as AOAC fibre)
How? DF = Complex landscape total high and low molar weight dietary fibre AOAC 2009.01 RS1 RS2 RS4 Resistant starch AOAC 2002.02 RS3 Classical total dietary fibre AOAC 985.29 Classical soluble high molar mass dietary fibre AOAC 991.43 Inulin/FOS AOAC 997.08 & AOAC 999.03 Polydextrose AOAC 2000.11 Galactooligosaccharide AOAC 2001.02 Resistant maltodextrin AOAC 2001.03
How? Complexity DF: Various extraction routes DF Pancreatic amylase/amg treatment Protease treatment Ethanol precipitation AOAC 985.29 Classical dietary fibre AOAC 2002.02 Resistant starch (37 C) RS remains present as DF Residue Filtration Filtrate AOAC 2001.03 DF containing supplemented resistant maltodextrin Gravimetry IHMWDF + SHMWDF Including resistant starch Removal ethanol Desalting GPC-RI LMWDF 2 analytical results
AOAC 2011.25 (instead of AOAC 991.43) The new total dietary fibre determination Pancreatic amylase/amg treatment Protease treatment residue filtration filtrate Ethanol precipitation residue filtration filtrate Removal ethanol desalting gravimetry gravimetry GPC-RI IHMWDF + RS1 + RS2 + RS3 + (partly) RS4 SHMWDF + (partly) RS4 LMWDF
Dietary fibre and prebiotic analyses Dietary fiber analyses Method Type DF included in the analysis AOAC 985.29 HMWDF AOAC 991.43 ihmwdf (incl. RS3) + shmwdf AOAC 2009.01 HMWDF (incl. RS) + LMWDF AOAC 2011.25 ihmwdf (incl. RS) + shmwdf + LMWDF AOAC 2002.02 Resistant starch AOAC 995.16 b-glucan Prebiotic analyses Method Dedicated prebiotic AOAC 997.08 Inulin/FOS AOAC 999.03 Inulin/FOS AOAC 2000.11 Polydextrose AOAC 2001.02 Galacto-oligosaccharides (GOS) AOAC 2001.03 Resistant maltodextrin Specific methods
Portfolio Dietary Fibres Analyte Matrix Method based on Classical Dietary Fibre Food AOAC 985.29 Classical Dietary Fibre (soluble & insoluble) Food AOAC 991.43 Total Dietary fibre (incl. LMWDF) Food AOAC 2009.01 Total Dietary fibre (incl. LMWDF) Food (high starch content) AOAC 2009.01 revised Total Dietary fibre (soluble, insoluble & LMWDF) Food AOAC 2011.25 Total Dietary fibre (cont. suppl. resistant maltodextrin) Food AOAC 2001.03 Pectin (as uronic acid) Food/Feed in-house b-glucan (cereals/grains) Food/Feed AOAC 995.16 b-glucan (yeasts/moulds) Ingredients in-house Resistant starch Food/Feed AOAC Various hydrocolloids Food/Feed In-house methods
Suggested decision tree for DF analysis Does the sample contain natural low molar weight dietary fibre or added dietary fibre ingredient NO YES, added fibre ingredients YES, natural low molar weight dietary fibres UNKNOWN With natural HMWDF With RS With GOS With Resistant maltodextrin With Inulin/FOS With Polydextrose With RS3 With RS1, RS2 or RS4 AOAC 985.29 or AOAC 991.43 AOAC 2009.01 AOAC 2009.01 (+ AOAC 2001.02 if %GOS in DF needed) AOAC 2001.03 AOAC 2009.01 or AOAC 2011.25
Summarizing Various definitions and currently accepted dietary fibre definition (EC directive 2008/100/EC) Which (food) components are considered as dietary fibre Health benefits related to dietary fiber consumption Difficulty to correctly determine all components in the dietary fibre by one method and the disadvantages of combining several methods. Decision tree for dietary fibre analysis
Carbohydrate Competence Centre Thank you contact us carbohydrates@eurofins.com yannisvrasidas@eurofins.com website www.carbohydratestesting.com
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