nutrient loss in high moisture forage Department of Animal Sciences IFAS

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Reducing dry matter and nutrient loss in high moisture forage K. Arriola, O. Queiroz, E. Muniz, J. Romero, M. Zarate, J. Hamie and A.T. Adesogan Department of Animal Sciences IFAS

Introduction Silage heating and DM losses (shrinkage) Waste feed and $$$ Decrease nutrients in feed Reduce forage availability Reduce animal a performance Reduce profit.

Effect of feeding spoiled silage on DMI and NDF digestibility 18 (Whitlock et al., 2000) 17 a DMI NDFD 16 b 15 14 bc c 13 0 5.4 10.7 14.7 Loss of $100 per cow per year if milk yield decreases by 2-3 lb/d; $100,000 per year in a 1000-cow dairy

Spoiled silage issues Increase DM loss - $$$$ Mold & yeasts Decreased quality Reduced intake and milk yield Diseases (bloody gut, aspergillosis etc) Mycotoxins Pathogens (Listeria, Clostridia, etc)

Approaches to reducing DM losses and spoilage 1. Using additives and inoculants 2. Keeping oxygen out / sealing well

Silo seal integrity The quality of the plastic seal is important to minimize losses in bunker, pile, and bag silos and round bale haylages

No good alternatives to plastic for covering bunkers/piles exist Muck, 2006 No one Oats Plas stic Mo olasse es Sa awdus st Lime

Types of covers Typical covers are 6 to 8 mil polyethylene sheets held in place by tires. A new Silostop film with 1/20 oxygen permeability of normal plastic has been developed

Silostop cover options 2 step (Silostop film + plastic) 1 step (Silostop gold film is incorporated into ) Sand bags are used to weigh down the plastic

Silostop Potential savings Bolsen, 2006 6 mil plastic Silostop Silage value $/ton 32.5 32.5 Silage in original top 3 ft, tons 288 288 Silage lost in original top 3ft, % ensiled 20 12 Cost of cover /sq. ft 3.5 10 Value of silage in original top 3 ft, $ 9360 9360 Value of silage lost in original 3 ft 1870 1120 Sealing cost 560 800 Net silage saved, $/silo 2270 2760 (Based on a 40 x 100 ft 2 bunker silo with a wet density of 48 lb/cu ft and an (Based on a 40 x 100 ft bunker silo with a wet density of 48 lb/cu ft and an assumed 8% reduction in spoiled silage in the top layer)

Silostop versus plastic Plastic Silostop Needs Plastic & tires Silostop, plastic, sandbags (2 step) Or Silostop & sand bags (1 step) Plastic needed Less More with 2 step Plastic cost Lower Higher Waste Possibly greater Possibly less Corrosion More on side walls Less on side walls Labor Similar, Similar, may be less Cover weight storage Tires are lighter but difficult to store Sand bags are heavy but easy to stack

Cover type effects on DM losses near the wall Control (8 mil plastic) Silostop (2 step) Silostop reduced spoilage in the top 6 inches near the wall but had little effect on losses in the middle of the bunker (Muck, 2007)

Results from Muck, 2007 Virtual elimination of visible spoilage with Silostop Biggest differences between the two systems have been at the top layer near the wall (shoulders). No cover effects on mold counts Silostop silage had lower ph and higher Lactate:Acetate ratio (improved fermentation)

Cover type effects on fiber digestibility (30-h NDFD) Silostop improved fiber digestibility at the shoulders McDonell and Kung, 2006

UF Experiment Cover type effects on silage quality

Treatments Normal plastic on top (6 mil) + tires Normal plastic on top and side walls + tires Silostop (2 step) + sand bags

Treatment silos (20 x 12 x 12 ft) 3 silos per treatment side wall Control Silo stop Control Silo stop Silo stop side wall Control side wall

Methods Normal plastic on top Normal plastic on top Silostop on top + and side walls normal plastic on top of silostop plastic and on side walls 3 bunkers were used for each cover type and 6 bags were placed in each bunker Pictures are show only the bag locations; these are not the bunkers from this trial

Positions of bags in bunkers Depths Top (1 foot below surface) Bottom (5 feet below surface) 5 ft 2 ft 2 ft Distance from side wall

RESULTS S Control treatment, The top layer of all 3 replicate silos for this treatment looked bad with a distinct spoilage crust on the top layer Side wall treatment, The top layer of all 3 replicate silos for this treatment looked bad with a distinct spoilage crust on the top layer Silostop treatment, The top layer of 1 replicate silo looked excellent with no spoilage crust, 1 was average and 1 was bad

Effect of cover Control side wall Silostop P value DM, % 30.3 31.0 31.2 0.71 ph 3.84 3.88 3.81 0.65 Lactic acid, % 5.64 4.85 4.60 0.09 Acetic acid, % 2.96 3.16 3.25 0.78 Ammonia, % of Total N 0.58 0.63 0.55 0.25 No effect on the fermentation

Effect of cover Control Side Wall Silostop P value DM loss, % 13.1 12.8 12.0 0.92 Yeasts, log cfu/g 3.14 3.22 3.24 0.87 Molds, log cfu/g 3.24 3.40 3.19 0.66 Density, lb /ft 3 22.3 23.8 21.7 0.56 Aerobic stability, h 79.6 98.7 71.6 0.66 Thickness of top spoilage layer, inches 3.2 3.6 1.1 0.03 Silostop reduced top spoilage layer thickness; No other effect cover effects detected

Effect of distance from side wall 5 feet 2 feet P value DM, % 31.6 30.1 0.11 ph 3.83 3.86 0.66 Lactic acid, % 493 4.93 5.14 060 0.60 Acetic acid, % 2.91 3.34 0.20 Ammonia, % of total N 0.58 0.59 0.79 No effect on fermentation indicators

Effect of distance from side wall 5 feet 2 feet P value Aerobic stability, h 66.3 100.3 0.18 DM loss, % 11.7 13.6 0.38 Yeast, cfu/g 3.16 3.24 0.65 Mold, cfu/g 320 3.20 335 3.35 046 0.46 Density, kg/m 3 354.8 369.4 0.57 No effect on spoilage indicators

Effect of depth Bottom Top P value DM loss, % 89 8.9 a 16.4 b 0.001001 Yeasts, log cfu/g 317 3.17 323 3.23 072 0.72 Molds, log cfu/g 3.22 3.33 0.57 Aerobic stability, h 95.1 71.4 0.35 Greater shrinkage at the top; No effect on the spoilage indicators

Effect of depth Bottom Top P value DM, % 32.0 a 29.7 b 0.019 ph 3.69 a 3.99 b <.0001 Lactic acid, % 6.32 a 3.75 b <.0001 Acetic acid, % 229 2.29 a 395 3.95 b <.0001 Ammonia, % of total ln 0.52 a 0.65 b 0.002 Poorer fermentation at the top

Conclusions Silostop reduced the thickness of the top spoilage layer; Cover type had no other effects No differences due to distance from the side wall were detected. Dry matter loss, ph, acetic acid, and ammonia production were lower at the bottom, but lactic acid was greater at the bottom. Therefore, bottom samples had better fermentation and less shrinkage than top samples.

Cover type effects Proper covering is essential to optimize silage storage Silostop film reduced thickness of the top spoilage layer but did not affect the fermentation or bunk life In other studies, silostop was more effective at preserving silage at the shoulders of large bunkers

Silage distance from side wall: Summary Silos where the width is over 2 times the tractor width) Silage near side walls is not as well packed and fermented as silage in the middle Cover side walls with plastic Silos where the width is Width is less than 1.5 times the tractor width) Silage near side walls or in the middle of narrow silos are similarly packed and fermented Covering side walls is optional

Silage depth: summary Silage in the top layer is wetter and is poorly fermented compared to silage in lower layers Therefore pack the top layer more than lower layers and cover immediately

Acknowledgements Funding was provided by Southeast Milk Check-Off Bruno Rimini Ag Ltd. We appreciate the help of the UF Dairy Unit staff with this study