MD1-74-ECO. MemGold Eco Screen Combo Value Pack (MD1-39-ECO & MD1-63-ECO)

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MD1-74-ECO MemGold Eco Screen Combo Value Pack (MD1-39-ECO & MD1-63-ECO) 1

MemGold TM Eco Screen MD1-39-ECO MemGold* is based on crystallization conditions data mined from the Protein Data Bank and is a rationalized sparse matrix screen containing 96 conditions covering a range of ph, PEGs and salt additives. MD1-39-ECO is presented as 96 x 10 ml conditions. Sodium cacodylate has been replaced by MES in this version of the kit. Features of MemGold Eco Screen: Data mined from 300 crystallization conditions. Covers conditions for approximately 121 α- helical type membrane proteins. Addresses the diversity of membrane proteins studied, with many more transporters and channels and both α- and β-types. Does not contain cacodylate or azide. Introduction Recent years have seen a steady increase in the number of membrane protein structures solved and there are now (2008) approximately 130 different membrane protein structures in the PDB and over 300 crystallization conditions. The diversity of proteins has increased, with many more transporters and channels now in the database and a good number of both α- and β- types. Work by Dr. Simon Newstead in the group of Prof. So Iwata at Imperial College, London (2004-2009), produced a new sparse matrix screen for membrane proteins, called MemGold. MemGold is based on the conditions mined from the PDB and contains 96 conditions covering a range of ph, PEGs and salt additives. MP families. The proportion of structures belonging to each of the eight MP families in the database is shown. Bacterial rhodopsins (blue), GPCR (red), channels (black), transporters (green), photosynthetic and light harvesting complexes (purple), ATPases (orange), respiratory complexes (brown), others (DsbB-DsbA oxidase, intramembrane proteases, membrane-associated proteins in eicosanoid, and gluththione metabolism [MAPEG]) (olive). Success stories: Crystals of Membrane proteins grown with MemGold screens 1

Formulation Notes: MemGold reagents are formulated using ultrapure water (>18.0 MΩ) and are sterile-filtered using 0.22 μm filters. No preservatives are added. Final ph may vary from that specified on the datasheet. Molecular Dimensions will be happy to discuss the precise formulation of individual reagents. Individual reagents and stock solutions for optimization are available from Molecular Dimensions. Enquiries regarding MemGold formulation, interpretation of results or optimization strategies are welcome. Please e- mail, fax or phone your query to Molecular Dimensions. Contact and product details can be found at www.moleculardimensions.com References. Newstead, S., Ferrandon, S., Iwata, S. Protein Science 2008, 17(3) Rationalizing alpha-helical membrane protein crystallization. * Developed by Dr. S. Newstead (So Iwata lab), 2004-2009, Imperial College, London and manufactured under licence by Molecular Dimensions Ltd. 2

MemGold Eco Screen Conditions 1-48 (Box 1) MD1 39-ECO Tube ID Conc. Units Salt Conc. Units Buffer ph Conc. Units Precipitant 1-1 2.2 M Ammonium sulfate 0.08 M Sodium citrate 5.2 1-2 1.2 M Sodium citrate tribasic dihydrate 0.01 M Tris 8.0 1-3 0.015 M Tricine 8.5 24 % w/v PEG 4000 1-4 0.36 M Sodium chloride 0.015 M Sodium phosphate 7.0 9.9 % w/v PEG 4000 1-5 0.3 M Sodium chloride 0.01 M Tris 8.0 27.5 % w/v PEG 4000 1-6 0.225 M MES/Bis-Tris 6.6 6.6 % w/v PEG 6000 1-7 0.1 M Ammonium sulfate 0.1 M HEPES 7.5 12 % w/v PEG 4000 22 % v/v Glycerol 1-8 0.01 M Magnesium sulfate heptahydrate 0.02 M MES 6.5 7.7 % w/v PEG 1500 0.02 M Sodium chloride 0.02 M Calcium chloride dihydrate 1-9 2.5 M Ammonium sulfate 0.05 M HEPES 7.5 1-10 1.1 M Sodium citrate tribasic dihydrate 0.0665 M HEPES 7.5 1-11 3.3 M Ammonium sulfate 0.15 M Potassium phosphate 6.5 1-12 0.1 M Magnesium acetate tetrahydrate 0.1 M Sodium citrate 5.8 14 % w/v PEG 5000 MME 1-13 0.1 M Soduim chloride 0.02 M Sodium citrate 5.6 11 % w/v PEG 3350 1-14 0.1 M Sodium chloride 0.02 M Sodium citrate 5.6 5.5 % w/v PEG 3350 1-15 0.05 M Calcium chloride dihydrate 0.1 M Tris 8.2 32 % v/v PEG 400 0.05 M Barium chloride dihydrate 1-16 0.05 M Sodium chloride 0.1 M Sodium phosphate 6.2 16 % w/v PEG 4000 1-17 0.1 M Magnesium chloride hexahydrate 0.03 M Tris-HCl 8.2 19 % w/v PEG 4000 1-18 0.2 M Sodium chloride 0.025 M HEPES 7.5 13 % w/v PEG 4000 1-19 0.1 M HEPES 7.5 11 % w/v PEG 3350 1-20 0.1 M Sodium chloride 0.02 M KMES 6.7 6.6 % w/v PEG 4000 1-21 0.1 M Potassium chloride 0.02 M Tris 7.0 20 % w/v PEG 4000 1-22 0.05 M Magnesium chloride hexahydrate 0.1 M MES 6.7 6.6 % w/v PEG 3350 1-23 0.2 M Potassium chloride 0.1 M Sodium citrate 5.5 37 % v/v Pentaerythritol propoxylate (5/4 PO/OH) 1-24 0.1 M Tris 8.0 5.5 % w/v PEG 4000 1-25 0.1 M Sodium chloride 0.02 M Tris 7.0 7.7 % w/v PEG 4000 1-26 0.1 M Magnesium chloride hexahydrate 0.1 M Tris 7.5 22 % v/v PEG 400 1-27 0.04 M Sodium chloride 0.04 M Tris 8.0 27 % v/v PEG 350 MME 1-28 0.05 M Sodium chloride 0.1 M Sodium citrate 6.0 22 % v/v PEG 400 0.02 M Magnesium chloride hexahydrate 1-29 0.1 M Sodium acetate 5.5 8.8 % w/v PEG 2000 MME 1-30 0.4 M Ammonium acetate 8.0 13 % w/v PEG 2000 MME 1-31 0.02 M Bis-Tris 7.0 15 % w/v PEG 2000 1-32 0.1 M Sodium chloride 0.02 M Tris 7.5 11 % w/v PEG 1500 0.1 M Magnesium chloride hexahydrate 1-33 0.1 M Sodium chloride 0.1 M HEPES 8.0 11 % w/v PEG 1500 0.1 M Magnesium chloride hexahydrate 1-34 0.2 M Sodium acetate trihydrate 0.1 M HEPES 7.0 22 % w/v PEG 3000 0.2 M Potassium chloride 1-35 0.02 M Nickel(II) sulfate hexahydrate 0.01 M HEPES 7.0 33 % v/v Jeffamine M-600 1-36 0.15 M Sodium chloride 0.1 M Tris 8.0 13 % w/v PEG 6000 1-37 0.2 M Calcium chloride dihydrate 0.1 M HEPES 7.5 53 % v/v PEG 400 1-38 0.05 M Magnesium acetate tetrahydrate 0.05 M Sodium acetate 5.0 28 % v/v PEG 400 1-39 0.05 M HEPES 7.5 22 % w/v PEG 4000 1-40 0.2 M Calcium chloride dihydrate 0.1 M Tris-HCl 8.0 44 % v/v PEG 400 1-41 0.05 M Magnesium acetate tetrahydrate 0.05 M Sodium acetate 5.4 24 % v/v PEG 400 1-42 0.2 M Calcium chloride dihydrate 0.1 M MES 6.5 26 % v/v PEG 350 MME 1-43 0.1 M Potassium chloride 0.1 M Tris 8.5 39 % v/v PEG 400 1-44 0.05 M Magnesium chloride hexahydrate 0.1 M Glycine 9.0 22 % v/v PEG 400 1-45 0.1 M Ammonium sulfate 0.1 M Glycine 3.8 28 % v/v Triethylene glycol (TEG) 1-46 0.15 M Sodium formate 0.1 M HEPES 7.2 18 % w/v PEG 3350 1-47 0.2 M Sodium acetate 6.8 8.8 % w/v PEG 6000 1-48 0.2 M Potassium chloride 0.1 M MES 6.5 18 % w/v PEG 6000 3

MemGold Eco Screen Conditions 1-48 (Box 2) MD1 39-ECO Tube ID Conc. Units Salt Conc. Units Buffer ph Conc. Units Precipitant 2-1 0.22 M Sodium citrate tribasic dihydrate 0.1 M Tris 8.0 35 % v/v PEG 400 2-2 0.1 M Sodium acetate 4.5 17 % v/v PEG 400 2-3 1.0 M Lithium sulfate 0.02 M Tris 8.5 1.8 % w/v PEG 8000 2-4 0.02 M Tris 7.5 22 % v/v PEG 500 MME 2-5 0.05 M Sodium chloride 0.02 M Glycine 10.0 33 % w/v PEG 1000 2-6 0.2 M Magnesium chloride hexahydrate 0.1 M Tris 8.5 25 % w/v PEG 4000 2-7 0.2 M Magnesium chloride hexahydrate 0.1 M MES 6.5 31 % w/v PEG 2000 2-8 0.64 M Sodium acetate 4.6 18 % w/v PEG 3350 2-9 0.1 M Sodium chloride 0.1 M Tris-HCl 8.0 33 % v/v PEG 400 0.1 M Cadmium chloride hemi(pentahydrate) 2-10 0.1 M BICINE 8.9 31 % w/v PEG 2000 2-11 0.05 M Sodium sulfate 0.05 M Tris 8.5 35 % v/v PEG 400 0.05 M Lithium sulfate 2-12 0.1 M Sodium chloride 0.05 M Glycine 9.5 33 % v/v PEG 300 2-13 0.3 M Magnesium nitrate hexahydrate 0.1 M Tris 8.0 23 % w/v PEG 2000 2-14 0.12 M Lithium sulfate 0.02 M Tris 7.5 20 % v/v PEG 300 0.1 M Sodium citrate 5.0 2-15 0.1 M Sodium chloride 0.12 M Tris 9.4 20 % v/v PEG 400 2-16 0.2 M Sodium chloride 0.1 M HEPES 7.0 22 % v/v PEG 500 MME 2-17 0.1 M Sodium chloride 0.1 M Tris 8.0 21 % v/v PEG 400 0.325 M Sodium acetate trihydrate 2-18 0.02 M Sodium citrate tribasic dihydrate 0.08 M Sodium phosphate 6.2 18 % w/v PEG 2000 2-19 0.02 M Potassium nitrate 0.03 M Potassium citrate 6.5 7.7 % w/v PEG 4000 2-20 0.1 M Sodium chloride 0.1 M Tris 8.5 30 % w/v PEG 2000 MME 0.005 M Magnesium chloride hexahydrate 2-21 0.2 M Calcium chloride dihydrate 0.1 M HEPES 7.0 33 % v/v PEG 400 2-22 0.1 M Calcium chloride dihydrate 0.1 M Tris 6.5 13 % w/v PEG 2000 MME 2-23 0.2 M Ammonium sulfate 0.02 M Sodium acetate 4.0 33 % v/v PEG 200 0.02 M Sodium chloride 2-24 0.07 M Sodium chloride 0.05 M Sodium citrate 4.5 22 % v/v PEG 400 2-25 0.2 M Ammonium sulfate 0.1 M Sodium acetate 4.6 28 % v/v PEG 500 MME 2-26 0.05 M Glycine 9.0 55 % v/v PEG 400 2-27 0.1 M Magnesium chloride hexahydrate 0.1 M Tris 8.5 33 % v/v PEG 400 0.1 M Sodium chloride 2-28 0.05 M Citric acid 19 % w/v PEG 1000 0.1 M Lithium sulfate 0.05 M Sodium phosphate dibasic dihydrate 2-29 0.2 M Magnesium chloride hexahydrate 0.025 M Sodium citrate 4.0 33 % v/v PEG 400 0.1 M Potassium chloride 2-30 0.05 M Zinc acetate dihydrate 0.05 M MES 6.1 11 % w/v PEG 8000 2-31 0.3 M Magnesium nitrate hexahydrate 0.1 M Tris 8.0 22 % w/v PEG 8000 2-32 0.1 M Sodium chloride 0.1 M MES 6.5 33 % v/v PEG 400 4 % v/v Ethylene glycol 2-33 0.05 M Sodium chloride 0.1 M Sodium citrate 5.5 26 % v/v PEG 400 2-34 0.1 M Lithium sulfate 0.1 M Glycine 9.3 30 % v/v PEG 400 2-35 0.15 M Potassium citrate tribasic monohydrate 22 % w/v PEG 6000 0.05 M Lithium citrate tribasic tetrahydrate 0.1 M Sodium phosphate monobasic monohydrate 2-36 0.001 M Zinc sulfate heptahydrate 0.05 M HEPES 7.8 28 % v/v PEG 600 2-37 0.1 M Sodium chloride 0.1 M Sodium phosphate 7.0 33 % v/v PEG 300 2-38 0.1 M Sodium chloride 0.05 M BICINE 9.0 33 % v/v PEG 300 2-39 0.05 M Zinc acetate dihydrate 0.1 M MES 6.0 6.6 % w/v PEG 8000 6 % v/v Ethylene glycol 2-40 0.2 M Lithium sulfate 0.1 M Sodium citrate 3.5 28 % v/v PEG 400 2-41 0.1 M Sodium chloride 0.1 M Tris 7.5 11 % w/v PEG 4000 2-42 0.05 M Lithium sulfate 0.1 M Tricine 7.4 7 % w/v PEG 3000 2-43 0.2 M Calcium chloride dihydrate 0.1 M MES 6.5 33 % v/v PEG 400 2-44 1.0 M Sodium chloride 0.1 M Sodium citrate 6.0 28 % w/v PEG 4000 2-45 0.1 M HEPES 7.5 11 % w/v PEG 4000 2-46 0.002 M Zinc sulfate heptahydrate 0.08 M HEPES 7.0 25 % v/v Jeffamine ED-2003 2-47 0.001 M Cadmium chloride hemi(pentahydrate) 0.1 M MES 6.5 30 % v/v PEG 400 0.03 M Magnesium chloride hexahydrate 2-48 3.0 M Sodium chloride 0.1 M Bis-Tris propane 7.0 4

Abbreviations: Bicine; N,N-Bis(2-hydroxyethyl)glycine, HEPES; N-(2-hydroxyethyl)-piperazine-N'-2-ethanesulfonic acid, KMES; 2-(N-morpholino)ethanesulfonic acid potassium salt, MES; 2-(N-morpholino)ethanesulfonic acid, MME; Monomethylether, PEG; Polyethylene glycol, Tricine; N- [Tris(hydroxymethyl)methyl]glycine, Tris; 2-Amino-2-(hydroxymethyl)propane-1,3-diol, Tris HCl; 2-Amino-2-(hydroxymethyl)propane-1,3-diol, hydrochloride.] Manufacturer s safety data sheets are available from our website or by scanning the QR code here: Re-Ordering details: Catalogue Description Pack size Catalogue Code MemGold 96 x 10 ml MD1-39 MemGold HT-96 96 x 1 ml MD1-41 Eco screens MemGold Eco Screen 96 x 10 ml MD1-39-ECO MemGold HT-96 Eco Screen 96 x 1 ml MD1-41-ECO Green screens (contain fluorescent green dye- ideal for UV) MemGold Green screen 96 x 10 ml MD1-56 MemGold HT-96 Green screen 96 x 1 ml MD1-53 Combo Packs (MemGold + MemGold2) MemGold Combo Value Pack 2 x 96 x 10 ml MD1-74 MemGold HT96 Combo Value Pack 2 x 96 x 1 ml MD1-74-HT Single Reagents MemGold single reagents 100 ml MDSR-39-tube number MemGold HT-96 single reagents 100 ml MDSR-41-well number For MemGold stock solutions please visit the Optimization section on our website. 5

MemGold2 TM Eco Screen MD1-63-ECO MemGold2 TM - The latest innovation for crystallization of membrane proteins. This screen targets all alpha helical types of Prokaryotic and Eukaryotic membrane proteins. MD1-63-ECO is a cacodylate-free targeted sparse matrix presented as 96 x 10 ml conditions. Features of MemGold2: A brand new set of 96 of the most recent alpha-helical membrane protein crystallization conditions cacodylate-free. Particularly suited for Prokaryotic and Eukaryotic alpha-helical membrane proteins. A great addition to any membrane protein lab. Works with MemGold, MemStart, MemSys & MemPlus. Screening over a wider range of ph s (4-10). Addition of small MW PEGs. Can be used in conjunction with Lipidic Sponge Phase and/or Lipidic Cubic Phases. Introduction: In 2008 Molecular Dimensions released MemGold (1) - a rationalized sparse matrix type membrane protein crystallization screen. MemGold was based on the crystallization conditions for 121 alpha helical Membrane Proteins deposited in the PDB. Since MemGold, the number of structures has more than doubled. In response to this, MemGold2 (2) has been developed. MemGold2 includes a further 96 crystallization conditions from unique alpha helical Membrane Protein structures including channel and transporter structures, GPCRs and ATPases. It is suitable for both Prokaryotic and Eukaryotic alpha helical membrane proteins. Formulation Notes: MemGold2 reagents are formulated using ultrapure water (>18.0 MΩ) and are sterile-filtered using 0.22 µm filters. No preservatives are added. Final ph may vary from that specified on the datasheet. Molecular Dimensions will be happy to discuss the precise formulation of individual reagents. Individual reagents and stock solutions for optimization are available from Molecular Dimensions. Enquiries regarding MemGold2 formulation, interpretation of results or optimization strategies are welcome. Please e-mail, fax or phone your query to Molecular Dimensions. Contact and product details can be found at www.moleculardimensions.com *References: (1) Newstead, S., Ferrandon, S., and Iwata, S. Rationalizing alpha-helical membrane protein crystallization Volume 17, Issue 3, pages 466 472, March 2008 - Protein Science, 2008 - Wiley Online Library. (2) Parker, J. and Newstead, S. Current trends in alpha helical membrane protein crystallization: an update, Protein Science, 2012, 21(9):1358-65. Examples of membrane protein crystals grown using MemGold2 and the structure of a membrane transport protein (previously solved using MemGold). 1

MemGold2 Eco Screen Conditions 1-48 (Box 1) MD1 63-ECO Tube # Conc. Salt Conc. Buffer ph Conc. Precipitant 1-1 0.2 M Magnesium chloride hexahydrate 0.1 M Tris 7.5 14 % v/v PEG 500 MME 0.005 M Cadmium chloride hemi(pentahydrate) 1-2 0.1 M Potassium acetate 0.02 M Tris 7.0 44 % w/v PEG 3000 0.01 M Potassium chloride 1-3 0.08 M Magnesium sulfate heptahydrate 0.02 M MES 6.0 10 % w/v PEG 1450 0.02 M Sodium chloride 1-4 0.04 M Magnesium sulfate heptahydrate 0.02 M MES 6.5 8 % w/v PEG 1450 0.02 M Sodium chloride 1-5 0.05 M Sodium sulfate 0.05 M Tris 8.5 32 % v/v PEG 400 0.05 M Lithium chloride 1-6 0.1 M Sodium phosphate monobasic monohydrate 0.1 M Bis-Tris propane 7.5 10 % w/v PEG 3350 0.1 M Potassium phosphate dibasic 1-7 0.1 M Sodium chloride 0.1 M ADA 6.5 11.5 % w/v PEG 4000 0.1 M Lithium sulfate 1-8 0.1 M Lithium chloride 0.1 M Sodium acetate 4.5 30 % v/v PEG 400 0.1 M Cadmium chloride hemi(pentahydrate) 1-9 0.2 M Ammonium sulfate 0.1 M Sodium citrate 6.0 20 % w/v PEG 2000 0.1 M Sodium chloride 1-10 0.2 M Lithium sulfate 0.1 M HEPES 7.0 31 % v/v PEG 400 0.1 M Sodium chloride 1-11 0.2 M Ammonium phosphate monobasic 0.1 M Sodium citrate 4.5 32 % v/v PEG 400 0.1 M Ammonium sulfate 1-12 0.05 M Sodium citrate tribasic dihydrate 0.08 M Bis-Tris 6.0 14 % w/v PEG 4000 0.12 M Potassium chloride 1-13 0.1 M Sodium chloride 0.01 M MES 6.5 19 % w/v PEG 1000 0.15 M Ammonium sulfate 1-14 0.01 M Nickel(II) sulfate hexahydrate 0.1 M Sodium citrate 6.0 18 % w/v PEG 2000 MME 1-15 0.02 M Magnesium chloride hexahydrate 0.02 M MES 6.0 3.5 % w/v PEG 3350 1-16 0.02 M Sodium chloride 0.05 M MES 5.5 14 % v/v PEG 350 MME 1-17 0.025 M Magnesium chloride hexahydrate 0.02 M MOPS 7.0 35 % v/v PEG 500 MME 1-18 0.03 M Magnesium chloride hexahydrate 0.1 M MES 6.5 28 % v/v PEG 400 1-19 0.04 M Sodium chloride 0.04 M Tris 8.0 25 % v/v PEG 350 MME 1-20 0.04 M Magnesium acetate tetrahydrate 0.1 M MES 6.0 36 % v/v MPD 1-21 0.05 M Zinc acetate dihydrate 0.05 M ADA 6.3 11 % w/v PEG 8000 1-22 0.05 M Magnesium acetate tetrahydrate 0.1 M MES 6.5 26 % v/v PEG 400 1-23 0.05 M Magnesium acetate tetrahydrate 0.1 M Glycine 9.5 32 % v/v PEG 400 1-24 0.066 M Sodium chloride 0.02 M Tris 7.5 3 % w/v PEG 4000 1-25 0.075 M Magnesium chloride hexahydrate 0.1 M MES 6.5 30 % w/v PEG 2000 MME 1-26 0.08 M Magnesium acetate tetrahydrate 0.1 M Sodium citrate 6.0 14 % w/v PEG 5000 MME 1-27 0.01 M Zinc acetate dihydrate 0.1 M MES 6.0 1.5 M Ammonium sulfate 1-28 0.087 M Ammonium sulfate 0.5 M Tris 7.0 22 % v/v PEG 250 DME 1-29 0.1 M Magnesium chloride hexahydrate 0.1 M Tris 7.5 13 % w/v PEG 8000 1-30 0.1 M Magnesium formate dihydrate 0.1 M MOPS 7.0 17 % w/v PEG 3350 1-31 0.1 M Potassium chloride 0.1 M Bis-Tris 6.0 18 % w/v PEG 4000 1-32 0.1 M Potassium chloride 0.1 M Potassium phosphate 7.5 18 % v/v PEG 200 1-33 0.1 M Magnesium acetate tetrahydrate 0.1 M MES 6.0 22 % w/v PEG 4000 1-34 0.1 M Calcium acetate hydrate 0.1 M MES 6.0 22 % w/v PEG 8000 1-35 0.1 M Ammonium sulfate 0.1 M HEPES 8.5 23 % w/v PEG 3350 1-36 0.1 M Potassium chloride 0.1 M MES 6.0 32 % v/v PEG 400 1-37 0.1 M Sodium chloride 0.1 M MES 6.5 36 % v/v PEG 300 1-38 0.1 M Sodium chloride 0.1 M BICINE 9.0 45 % v/v PEG 500 MME 1-39 0.15 M Calcium chloride dihydrate 0.1 M Glycine 9.0 35 % v/v PEG 400 1-40 0.2 M Ammonium sulfate 0.05 M ADA 6.5 13 % w/v PEG 4000 1-41 0.2 M Choline chloride 0.1 M Tris 7.5 14 % w/v PEG 2000 MME 1-42 0.2 M Sodium chloride 0.05 M MOPS 7.0 19 % w/v PEG 6000 1-43 0.05 M Sodium chloride 0.05 M MOPS 7.0 19 % w/v PEG 6000 1-44 0.2 M Magnesium formate dihydrate 0.05 M Tris 8.0 19 % w/v PEG 3350 1-45 0.2 M Calcium chloride dihydrate 0.1 M MES 5.0 20 % v/v PEG 350 MME 1-46 0.2 M Ammonium nitrate 0.05 M HEPES 7.0 20 % w/v PEG 3350 1-47 0.02 M Lithium chloride 0.02 M Glycine 10.0 33 % w/v PEG 1000 0.05 M Magnesium chloride hexahydrate 1-48 0.2 M Calcium acetate hydrate 0.1 M HEPES 7.0 24 % v/v PEG 400 2

MemGold2 Eco Screen Conditions 1-48 (Box 2) MD1 63-ECO Tube # Conc. Salt Conc. Buffer ph Conc. Precipitant 2-1 0.2 M Sodium acetate trihydrate 0.1 M MES 6.5 28 % v/v PEG 400 2-2 0.2 M Sodium chloride 0.05 M Calcium acetate 5.0 29 % v/v PEG 400 2-3 0.2 M Sodium chloride 0.1 M HEPES 7.0 29 % v/v PEG 400 2-4 0.2 M Ammonium formate 0.1 M Tris 7.0 31 % v/v Pentaerythritole ethoxylate (15/4 EO/OH) 2-5 0.2 M Ammonium sulfate 0.1 M Tris 8.5 35 % w/v PEG 3350 2-6 0.2 M Calcium acetate hydrate 0.1 M Sodium acetate 5.0 38 % v/v PEG 400 2-7 0.2 M Sodium chloride 0.1 M MOPS 7.5 38 % v/v PEG 400 2-8 2.0 M Ammonium sulfate 0.1 M MES 6.5 0.2 M Sodium chloride 2-9 0.225 M Ammonium sulfate 0.05 M Sodium acetate 4.0 12 % w/v PEG 4000 2-10 0.23 M Sodium chloride 0.05 M Sodium acetate 4.5 33 % v/v PEG 400 2-11 0.25 M Magnesium formate dihydrate 0.1 M MES 6.5 22 % w/v PEG 3000 2-12 0.25 M Magnesium chloride hexahydrate 0.1 M Tris 8.5 40 % w/v PEG 1000 2-13 0.3 M Lithium sulfate 0.1 M MES 6.5 25 % v/v PEG 400 2-14 0.3 M Ammonium formate 0.05 M Tris 9.0 33 % v/v PEG 500 MME 2-15 0.3 M Barium chloride dihydrate 0.1 M MES 6.0 34 % v/v PEG 400 2-16 0.32 M Lithium chloride 0.1 M Sodium citrate 5.5 14 % w/v PEG 4000 2-17 0.34 M Ammonium sulfate 0.1 M Sodium citrate 5.5 12 % w/v PEG 4000 2-18 0.35 M Lithium sulfate 0.1 M Sodium acetate 4.0 11 % v/v PEG 600 2-19 0.37 M Potassium nitrate 0.1 M MES 6.5 22 % v/v PEG 400 2-20 0.4 M Ammonium sulfate 0.1 M MES 6.5 10 % w/v PEG 3350 2-21 0.04 M Magnesium chloride hexahydrate 0.1 M HEPES 7.5 32 % v/v PEG 400 0.05 M Sodium chloride 2-22 0.4 M Potassium chloride 0.05 M HEPES 7.5 12 % v/v PEG 400 2-23 0.4 M Ammonium thiocyanate 0.1 M Sodium acetate 4.5 15 % w/v PEG 4000 2-24 0.4 M Sodium thiocyanate 0.1 M Sodium acetate 4.0 16 % w/v PEG 4000 2-25 0.5 M Potassium chloride 0.05 M HEPES 6.5 20 % v/v PEG 400 2-26 0.5 M Magnesium chloride hexahydrate 0.05 M Tris 7.5 21 % v/v PEG 350 MME 2-27 0.8 M Potassium formate 0.1 M Sodium acetate 5.0 11 % w/v PEG 4000 2-28 0.1 M MOPS 7.0 9 % w/v PEG 8000 2-29 0.1 M MES 6.0 11 % w/v PEG 20,000 2-30 0.1 M MES 6.5 13 % v/v PEG 400 2-31 0.1 M ADA 5.5 14 % w/v PEG 6000 2-32 0.05 M Tris 7.5 17 % v/v PEG 350 MME 2-33 0.07 M Sodium citrate 4.5 22 % v/v PEG 300 2-34 0.05 M ADA 6.5 24 % v/v PEG 400 2-35 0.1 M MES 6.5 24 % w/v PEG 1500 2-36 0.1 M HEPES 7.5 28 % v/v PEG 600 2-37 0.05 M Tris 8.5 28 % v/v PEG 400 2-38 0.1 M BICINE 9.0 30 % v/v PEG 400 2-39 0.1 M ADA 7.0 31 % v/v PEG 600 2-40 0.1 M Tris 8.5 32 % v/v PEG 500 MME 2-41 0.1 M HEPES 7.5 33 % v/v PEG 400 2-42 0.18 M Sodium citrate 4.0 34 % w/v PEG 3350 2-43 0.1 M Tris 8.5 44 % v/v PEG 200 2-44 0.1 M Tris 8.0 65 % v/v MPD 2-45 2.75 M Ammonium chloride 0.025 M Bis-Tris 7.0 2-46 2.8 M Ammonium chloride 0.075 M HEPES 7.5 2-47 3.0 M Ammonium sulfate 0.1 M MES 5.5 2-48 0.01 M HEPES 7.5 3.25 M 1,6-Hexanediol 3

Abbreviations: ADA; N-(2-Acetamido)iminodiacetic Acid, BICINE; N,N-Bis(2-hydroxyethyl)glycine, Bis-Tris; 2,2'-(Propane-1,3- diyldiimino)bis[2-(hydroxymethyl)propane-1,3-diol]. CHES; 2-(N-Cyclohexylamino)ethane sulfonic Acid, HEPES; N-(2- hydroxyethyl)-piperazine-n'-2-ethanesulfonic acid, MES; 2-(N-morpholino)ethanesulfonic acid, MME; Monomethylether, MOPS; 3-morpholinopropane-1-sulfonic acid, PEG; Polyethylene glycol, PEG DME; Poly(ethylene glycol) bis(carboxymethyl) ether, Tricine; N-[Tris(hydroxymethyl)methyl]glycine, Tris; 2-Amino-2-(hydroxymethyl)propane-1,3- diol. Manufacturer s safety data sheets are available from our website or by scanning the QR code here: Re-Ordering details: Catalogue Description Pack size Catalogue Code MemGold2 96 x 10 ml MD1-63 MemGold2 HT-96 96 x 1 ml MD1-64 Eco screens MemGold2 96 x 10 ml MD1-63-ECO MemGold2 HT-96 96 x 1 ml MD1-64-ECO Green screens (contain fluorescent green dye- ideal for UV) MemGold2 Green screen 96 x 10 ml MD1-63-GREEN MemGold2 HT-96 Green screen 96 x 1 ml MD1-64-GREEN Combo Packs (MemGold + MemGold2) MemGold Combo Value Pack 2 x 96 x 10 ml MD1-74 MemGold HT96 Combo Value Pack 2 x 96 x 1 ml MD1-74-HT Single Reagents MemGold2 single reagents 100 ml MDSR-63-tube number MemGold2 HT-96 single reagents 100 ml MDSR-64-well number For MemGold2 stock solutions please visit the Optimization section on our website. 4

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