MACS Marker Screen. MACS Marker Screen. Catalog antibodies. antibodies CD4 (VIT4) CD11b. MACS Marker Screen antibodies. Catalog. antibodies CD2 CD14
|
|
|
- Jasmin Beasley
- 5 years ago
- Views:
Transcription
1 MACS Marker Screen The performance of MACS Marker Screen was tested and compared with catalog reagents offered in single vials as liquid antibody reagents. The were reconstituted using 25 µl of deionized water each. Human peripheral blood mononuclear cells (PBMCs) were then added to each well and stained for 1 minutes at 4 C followed by two wash steps. The cells were then resuspended in 6 μl of PEB buffer, and analyzed using an MACSQuant Analyzer with an uptake volume of 25 µl. No significant differences in the staining patterns or median fluorescence intensity between the and liquid was observed. CD4 (VIT4) 25 CD11b 1 1¹ 1² CD4 (VIT4)-APC 1³ ¹ 1² CD4 (VIT4)-APC 1³ CD CD14 1 1¹ 1² CD11b-APC 1³ 1 1¹ 1² CD11b-APC 1³ CD3 1 1¹ 1² CD2-APC 1³ 1 1¹ 1² CD2-APC 1³ CD2 1 1¹ 1² CD14-APC 1³ 1 1¹ 1² CD14-APC 1³ 1 1¹ 1² CD3-APC 1³ 1 1¹ 1² CD3-APC 1³ ¹ 1² 1³ 1 1¹ 1² 1³ CD2-APC CD2-APC MACS Marker Screen May 216 1/5 Copyright 216 Miltenyi Biotec GmbH. All rights reserved.
2 CD21 CD45RA ¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ CD21-APC CD21-APC CD45RA-APC CD45RA-APC CD22 CD45RO ¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ CD22-APC CD22-APC CD45RO-APC CD45RO-APC CD38 CD ¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ CD38-APC CD38-APC CD56-APC CD56-APC CD41b CD ¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ CD41b-APC CD41b-APC CD57-APC CD57-APC CD45 CD ¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ CD45-APC CD45-APC CD64-APC CD64-APC MACS Marker Screen May 216 2/5 Copyright 216 Miltenyi Biotec GmbH. All rights reserved.
3 CD99 CD196 (CCR6) ¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ CD99-APC CD99-APC CD196 (CCR6)-APC CD196 (CCR6)-APC CD122 (IL-2Rβ) CD197 (CCR7) ¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ CD122 (IL-2Rβ)-APC CD122 (IL-2Rβ)-APC CD197 (CCR7)-APC CD197 (CCR7)-APC CD156c (ADAM1) CD ¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ CD156c (ADAM1)-APC CD156c (ADAM1)-APC CD222-APC CD222-APC CD161 CD226 (DNAM) ¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ CD161-APC CD161-APC CD226 (DNAM)-APC CD226 (DNAM)-APC CD185 (CXCR5) CD234 (DARC) ¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ CD185 (CXCR5)-APC CD185 (CXCR5)-APC CD234 (DARC)-APC CD234 (DARC)-APC MACS Marker Screen May 216 3/5 Copyright 216 Miltenyi Biotec GmbH. All rights reserved.
4 CD235a (Glycophorin-A) CD335 (NKp46) ¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ CD244 (2B4) CD235a (Glycophorin A)- APC CD235a (Glycophorin A)- APC Anti-CLA CD335 (NKp46)-APC CD335 (NKp46)-APC ¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ CD244 (2B4)-APC CD244 (2B4)-APC Anti-CLA-APC Anti-CLA-APC CD3e (IREM-2) Anti-CX3CR ¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ CD3e (IREM-2)-APC CD3e (IREM-2)-APC Anti-CX3CR1-APC Anti-CX3CR1-APC CD3f (IREM) Anti-HLA-DR ¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ CD3f (IREM)-APC CD3f (IREM)-APC Anti-HLA-DR-APC Anti-HLA-DR-APC CD314 (NKG2D) Anti-HLA-DR, DP, DQ ¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ 1 1¹ 1² 1³ CD314 (NKG2D)-APC CD314 (NKG2D)-APC Anti-HLA-DR, DP, DQ-APC Anti-HLA-DR, DP, DQ-APC MACS Marker Screen May 216 4/5 Copyright 216 Miltenyi Biotec GmbH. All rights reserved.
5 Anti-HLA-E ¹ 1² 1³ 1 1¹ 1² 1³ Anti-HLA-E-APC Anti-HLA-E-APC Anti-HLA-ABC ¹ 1² Anti-HLA-ABC-APC 1³ 1 1¹ 1² Anti-HLA-ABC-APC 1³ Anti-PSA-NCAM ¹ 1² Anti-PSA-NCAM-APC 1³ ¹ 1² Anti-PSA-NCAM-APC 1³ Anti-TCRα/β ¹ 1² 1³ 1 1¹ 1² 1³ Anti-TCRα/β-APC Anti-TCRα/β-APC Miltenyi Biotec GmbH Friedrich-Ebert-Straße Bergisch Gladbach Germany Phone Fax macs@miltenyibiotec.de Miltenyi Biotec provides products and services worldwide. Visit to find your nearest Miltenyi Biotec contact. Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use. MACS, the MACS logo, and MACSQuant are registered trademarks or trademarks of Miltenyi Biotec GmbH. Copyright 216 Miltenyi Biotec GmbH. All rights reserved. MACS Marker Screen May 216 5/5 Copyright 216 Miltenyi Biotec GmbH. All rights reserved.
Isn t it worth two bags?
Isn t it worth two bags? NEW from Miltenyi Biotec CryoMACS Freezing Bag CryoMACS Freezing Bag Designed for freezing and cryopreservation of blood components Overwrap bag protects both sample and freezing
The broadest portfolio for your innate lymphoid cell research
The broadest portfolio for your innate lymphoid cell research Tissue dissociation Cell separation Cell culture Flow cytometry Innate lymphoid cells Overview Innate lymphoid cells comprise various subpopulations
MACS Cell Pre-Enrichment for flow sorting and analysis
MACS Cell Pre-Enrichment for flow sorting and analysis Saves flow sorting time Improves cell viability Enhances quality of flow cytometry data MACS Cell Pre-Enrichment for flow sorting Time-saving cell
MACSxpress Technology Untouched cell isolation directly from blood products
MACSxpress Technology Untouched cell isolation directly from blood products MACSxpress Isolation Technology Fast untouched cell isolation in 25 min No density gradient centrifugation Flexible sample volumes
The broadest portfolio for your T cell research
quick guide The broadest portfolio for your T cell research Sample preparation Cell separation Cell culture Flow cytometry Mouse T cells Overview CD4 + T cell subsets Polarization reagents Naive CD4 +
In vitro human regulatory T cell suppression assay
Human CD4 + CD25 + regulatory T cell isolation, in vitro suppression assay and analysis In vitro human regulatory T cell suppression assay Introduction Regulatory T (Treg) cells are a subpopulation of
Direct ex vivo characterization of human antigen-specific CD154 + CD4 + T cells Rapid antigen-reactive T cell enrichment (Rapid ARTE)
Direct ex vivo characterization of human antigen-specific CD154 + CD4 + T cells Rapid antigen-reactive T cell enrichment (Rapid ARTE) Introduction Workflow Antigen (ag)-specific T cells play a central
In vitro human regulatory T cell expansion
- 1 - Human CD4 + CD25 + regulatory T cell isolation, Workflow in vitro expansion and analysis In vitro human regulatory T cell expansion Introduction Regulatory T (Treg) cells are a subpopulation of T
Efficient T cell activation and expansion. T Cell TransAct
Efficient T cell activation and expansion T Cell TransAct Introducing your new solution in T cell activation What is T Cell TransAct? This ready-to-use reagent provides an innovative method for physiological
In vitro human regulatory T cell expansion
- 1 - Human CD4 + CD25 + CD127 dim/- regulatory T cell Workflow isolation, in vitro expansion and analysis In vitro human regulatory T cell expansion Introduction Regulatory T (Treg) cells are a subpopulation
90 min 18 min. 45 min. 14 d
Isolation, cultivation, and expansion of Pan T cells from human PBMCs In vitro expansion of human Pan T cells Introduction T lymphocytes (T cells) play a central role in the adaptive immune system by controlling
IFN-γ Secretion Assay Detection Kit (PE) human
Miltenyi Biotec GmbH Friedrich-Ebert-Straße 68 51429 Bergisch Gladbach, Germany Phone +49 2204 8306-0 Fax +49 2204 85197 macs@miltenyibiotec.de Miltenyi Biotec Inc. 12740 Earhart Avenue Auburn CA 95602,
Clinical research Treg cell manufacture CliniMACS Cell Separation System and MACS GMP Products
Clinical research Treg cell manufacture CliniMACS Cell Separation System and MACS GMP Products Treg cell enrichment and depletion MACS GMP Fluorescent Antibodies for flow sorting Treg cell expansion Analysis
Find the fittest MACS ART Annexin V System
Find the fittest MACS ART Annexin V System Easy, fast, and specific depletion of unwanted apoptotic spermatozoa Improve quality of sperm preparations Obtain desired population within 30 minutes Proven
Recommended Protocols for Phospho Protein Detection in Human Cells
Recommended Protocols for Phospho Protein Detection in Human Cells Depending on subcellular localization of the phospho protein of interest, as well as epitope susceptibility to cell fixing and permeabilizing
Low Avidity CMV + T Cells accumulate in Old Humans
Supplementary Figure Legends Supplementary Figure 1. CD45RA expressing CMVpp65-specific T cell populations accumulate within HLA-A*0201 and HLA-B*0701 individuals Pooled data showing the size of the NLV/HLA-A*0201-specific
Supporting Information
Supporting Information Bellora et al. 10.1073/pnas.1007654108 SI Materials and Methods Cells. NK cells purified from peripheral blood mononuclear cells (PBMC) of healthy donors (Human NK Cell Isolation
Primary Adult Naïve CD4+ CD45RA+ Cells. Prepared by: David Randolph at University of Alabama, Birmingham
Primary Adult Naïve CD4+ CD45RA+ Cells Prepared by: David Randolph (drdrdr@uab.edu) at University of Alabama, Birmingham Goal: To obtain large numbers of highly pure primary CD4+ CD45RO- CD25- cells from
MagniSort Human CD4 Memory T cell Enrichment Kit Catalog Number: RUO: For Research Use Only. Not for use in diagnostic procedures.
Page 1 of 2 MagniSort Human CD4 Memory T cell Enrichment Kit RUO: For Research Use Only. Not for use in diagnostic procedures. Normal human peripheral blood mononuclear cells were unsorted (left) or sorted
Regulatory T cell research
Regulatory T cell research Unique kits for cell isolation Harmonized cell culture and expansion tools Convenient functional assay tools Reliable flow cytometry analysis Introduction The complete workflow
BD Flow Cytometry Reagents Multicolor Panels Designed for Optimal Resolution with the BD LSRFortessa X-20 Cell Analyzer
Multicolor Panels Designed for Optimal Resolution with the BD LSRFortessa X-2 Cell Analyzer Proper multicolor panel design takes into account fluorochrome brightness, antigen density, co-expression, and
Dendritic Cell - T Cell Immune Network
w w w. i m g e n e x. c o m BRIDGING INNATE & ADAPTIVE IMMUNITY Dendritic Cell - T Cell Immune Network Signaling Pathways IMGENEX provides the most extensive portfolio for in depth analysis of the Dendritic
Application Information Bulletin: Human NK Cells Phenotypic characterizing of human Natural Killer (NK) cell populations in peripheral blood
Application Information Bulletin: Human NK Cells Phenotypic characterizing of human Natural Killer (NK) cell populations in peripheral blood Christopher A Fraker, Ph.D., University of Miami - Miami, Florida
DURACLONE IF BE CERTAIN ABOUT THE RESPONSE. l res. a il n c n. For Research Use Only - Not for use in Diagnostic procedures
DURACLONE IF earch tria l res lc a om ic il n c n nio pa Yo ur BE CERTAIN ABOUT THE RESPONSE For Research Use Only - Not for use in Diagnostic procedures BE CERTAIN ABOUT THE RESPONSE The sensitive and
BD IMag Cell Separation System
BD IMag Cell Separation System Table of Contents Magnetic Cell Separation with BD IMag Particles.............................................................. 3 BD IMag Enrichment Protocol Flow Chart......................................................................
Supplemental Figure 1. IL-3 blockade with Fab CSL362 depletes plasmacytoid dendritic cells (pdcs), but not basophils, at higher doses.
Supplemental Figure 1. IL-3 blockade with Fab CSL362 depletes plasmacytoid dendritic cells (pdcs), but not basophils, at higher doses. Percentage of viable (A) pdcs (Sytox Blue-, Lin1-, HLADR+, BDCA2++)
CLINICAL USE OF CELLULAR SUBPOPULATION ANALYSIS IN BM
CLINICAL USE OF CELLULAR SUBPOPULATION ANALYSIS IN BM CANCER RESEARCH CENTRE, UNIVERSITY AND UNIVERSITY HOSPITAL OF SALAMANCA (SPAIN)( Sao Paulo, 18th of April, 2009 IDENTIFICATION OF HPC (I) 1.- In vivo
Annexin V APC Assay Kit
Annexin V APC Assay Kit Item No. 601410 www.caymanchem.com Customer Service 800.364.9897 Technical Support 888.526.5351 1180 E. Ellsworth Rd Ann Arbor, MI USA TABLE OF CONTENTS GENERAL INFORMATION 3 Materials
Myocardial regeneration autologous stem cell therapy
Myocardial regeneration autologous stem cell therapy 1 SPL/Agentur Focus Index Main patient cohort 3 Introduction 3 Cell therapy using adult, autologous stem cells 5 Clinical study results 6 Methodology
Applications for the MACSQuant Analyzer *
For research use only Enumeration of CD34/CD133 positive cells with the CD34/CD133 Enumeration Kit Applications for the MACSQuant Analyzer * Background The CD34 antigen is a single-chain transmembrane
Changing the way of generating engineered T cells. CliniMACS Prodigy T Cell Transduction Process
Changing the way of generating engineered T cells CliniMACS Prodigy T Cell Transduction Process CliniMACS Prodigy T Cell Transduction Process It is happening a new era of cell therapy Chimeric antigen
MagniSort Human CD4 T cell Enrichment Kit Catalog Number: RUO: For Research Use Only. Not for use in diagnostic procedures.
Page 1 of 2 MagniSort Human CD4 T cell Enrichment Kit RUO: For Research Use Only. Not for use in diagnostic procedures. Normal human peripheral blood mononuclear cells were unsorted (left) or sorted with
Cardiovascular research solutions. Conveniently groundbreaking
Cardiovascular research solutions Conveniently groundbreaking The easy workflow to pure cardiac cell types Easily dissociate neonatal hearts for the recovery of all major cardiac cell types Gentle yet
DISCOVER MORE WITH LESS SAMPLE. nanostring.com/3d
nanostring.com/3d DISCOVER MORE WITH LESS SAMPLE. WHY COMPROMISE? ncounter Vantage ASSAYS POWERED BY 3D BIOLOGY TECHNOLOGY Don t let sample volume limit your analytical aspirations. Quantify DNA, RNA,
DURACLONE RE THERE ARE EVENTS YOU CANNOT AFFORD TO MISS
DURACLONE RE THERE ARE EVENTS YOU CANNOT AFFORD TO MISS Your clinical research trial companion For Reseach Use Only - Not for use in Diagnostic procedures THERE ARE EVENTS YOU CANNOT AFFORD TO MISS Rare
Application of μmacs Streptavidin MicroBeads for the analysis of HIV-1 directly from patient plasma
Excerpt from MACS&more Vol 8 1/2004 Application of μmacs Streptavidin MicroBeads for the analysis of HIV-1 directly from patient plasma L. Davis Lupo and Salvatore T. Butera HIV and Retrovirology Branch,
All you need for your T cell research
All you need for your T cell research Gentle sample preparation Accurate cell separation Optimized cell culture Precise flow cytometry Products for isolation of mouse T cells T cells CD3ε MicroBead Kit
CliniMACS Technology in stem cell transplantation
Advancing clinical applications CliniMACS Technology in stem cell transplantation Graft manipulation T cell and B cell depletion Balance of GvT versus GvHD Fighting infections CliniMACS Technology in stem
Human Innate Lymphoid Cells: crosstalk with CD4 + regulatory T cells and role in Type 1 Diabetes
Joint Lab Meeting 03/03/2015 Human Innate Lymphoid Cells: crosstalk with CD4 + regulatory T cells and role in Type 1 Diabetes Caroline Raffin Bluestone Lab ILCs: Definition - Common lymphoid progenitor
Fluorochrome Panel 1 Panel 2 Panel 3 Panel 4 Panel 5 CTLA-4 CTLA-4 CD15 CD3 FITC. Bio) PD-1 (MIH4, BD) ICOS (C398.4A, Biolegend) PD-L1 (MIH1, BD)
Additional file : Table S. Antibodies used for panel stain to identify peripheral immune cell subsets. Panel : PD- signaling; Panel : CD + T cells, CD + T cells, B cells; Panel : Tregs; Panel :, -T, cdc,
Supplementary Figure 1. Immune profiles of untreated and PD-1 blockade resistant EGFR and Kras mouse lung tumors (a) Total lung weight of untreated
1 Supplementary Figure 1. Immune profiles of untreated and PD-1 blockade resistant EGFR and Kras mouse lung tumors (a) Total lung weight of untreated (U) EGFR TL mice (n=7), Kras mice (n=7), PD-1 blockade
MHC I Streptamer Manual
MHC I Streptamer Manual I) Staining of antigen-specific CD8 + T cells with reversible MHC I Streptamers and FACS isolation II) Isolation of antigen-specific CD8 + T cells with reversible MHC I Streps and
ab Lipid Peroxidation (MDA) Assay kit (Colorimetric/ Fluorometric)
Version 10b Last updated 19 December 2018 ab118970 Lipid Peroxidation (MDA) Assay kit (Colorimetric/ Fluorometric) For the measurement of Lipid Peroxidation in plasma, cell culture and tissue extracts.
Comprehensive evaluation of human immune system reconstitution in NSG. and NSG -SGM3 mouse models toward the development of a novel ONCO-HU
Comprehensive evaluation of human immune system reconstitution in NSG and NSG -SGM3 mouse models toward the development of a novel ONCO-HU xenograft model Aaron Middlebrook, 1 Eileen Snowden, 2 Warren
Excerpt from MACS&more Vol 17 1/2016. Kenji Miki¹, Koji Nagaoka¹, Hermann Bohnenkamp², Takayuki Yoshimoto³, Ryuji Maekawa¹*, and Takashi Kamigaki⁴
Excerpt from MCS&more Vol 17 1/2016 Dendritic cells pulsed with induce both OV-specific CD4 + and CD8 + T cells and cause antitumor effects in a mouse model of lymphoma Kenji Miki¹, Koji Nagaoka¹, Hermann
Supplementary Figure 1. Ex vivo IFNγ production by Tregs. Nature Medicine doi: /nm % CD127. Empty SSC 98.79% CD25 CD45RA.
SSC CD25 1.8% CD127 Empty 98.79% FSC CD45RA CD45RA Foxp3 %IFNγ + cells 4 3 2 1 + IL-12 P =.3 IFNγ p=.9 %IL-4+ cells 3 2 1 IL-4 P =.4 c %IL-1 + cells IFNγ 4 3 2 1 Control Foxp3 IL-1 P =.41.64 4.76 MS 2.96
In vitro expansion of mouse CD4 + T cells
Direct ex vivo isolation, cultivation and expansion of CD4 + T cells from mouse spleen In vitro expansion of mouse CD4 + T cells Introduction CD4 + T helper (TH) cells play a central role in the adaptive
For research or further manufacturing use only. Not for injection or diagnostic procedures.
PRIME-XV T cell Expansion XSFM PRIME-XV T Cell Expansion XSFM is a xeno-free, serum-free medium optimized for the activation and expansion of human T lymphocytes. This medium contains gentamicin and requires
CyTOF analyses in rheumatoid arthritis. Deepak Rao, MD PhD Rheumatology, Immunology, Allergy, BWH
CyTOF analyses in rheumatoid arthritis Deepak Rao, MD PhD Rheumatology, Immunology, Allergy, BWH Agenda: CyTOF analyses Analysis of synovial tissue Panel for analysis of synovial cells Comparison of CyTOF
Instructions. Fuse-It-mRNA easy. Shipping and Storage. Overview. Kit Contents. Specifications. Note: Important Guidelines
Membrane fusion is a highly efficient method for transfecting various molecules and particles into mammalian cells, even into sensitive and primary cells. The Fuse-It reagents are cargo-specific liposomal
91154 PRIME-XV T Cell CDM Liquid 1 L Additional package sizes are available at request
PRIME-XV T CELL CDM PRIME-XV T Cell CDM is a ready-to-use chemically-defined, animal component-free medium. It is optimized and designed for the culture of T cells of human origin and recommended for use
Plasma Membrane Protein Extraction Kit
ab65400 Plasma Membrane Protein Extraction Kit Instructions for Use For the rapid and sensitive extraction and purification of Plasma Membrane proteins from cultured cells and tissue samples. This product
Fast and Easy Isolation of T Cells
Fast and Easy Isolation of T Cells Isolate T Cells In As Little As 25 Minutes Isolate whole T cell populations as well as various T cell subsets with high purity and recovery using the fast and easy T
Intracellular (Total) ROS Activity Assay Kit (Red)
Intracellular (Total) ROS Activity Assay Kit (Red) Catalog Number KA2525 200 assays Version: 04 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General
ab Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma
Version 1 Last updated 25 May 2018 ab228565 Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma For the isolation/detection of exosomes from human plasma, urine or cell culture media. This product
MHC MULTIMER PROFICIENCY PANEL 2017
MHC MULTIMER PROFICIENCY PANEL 2017 August 2017 CONTACT Charlotte Halgreen ProficiencyPanel@immudex.com FOR MORE INFORMATION www.proficiencypanel.com MHC MULTIMER PROFICIENCY PANEL 2017 This report summarizes
b-galactosidase Staining Kit
b-galactosidase Staining Kit Catalog No. 35001 (version B) Active Motif North America 1914 Palomar Oaks Way, Suite 150 Carlsbad, California 92008, USA Toll free: 877 222 9543 Telephone: 760 431 1263 Fax:
Annexin V-APC/7-AAD Apoptosis Kit
Annexin V-APC/7-AAD Apoptosis Kit Catalog Number KA3808 100 assays Version: 04 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information... 4
T H 1, T H 2 and T H 17 polarization of naïve CD4 + mouse T cells
A complete workflow for cell preparation, isolation, polarization and analysis T H 1, T H 2 and T H 17 polarization of naïve CD4 + mouse T cells Introduction Workflow CD4 + T helper (T H) cells play a
CD25-PE (BD Biosciences) and labeled with anti-pe-microbeads (Miltenyi Biotec) for depletion of CD25 +
Supplements Supplemental Materials and Methods Depletion of CD25 + T-cells from PBMC. Fresh or HD precultured PBMC were stained with the conjugate CD25-PE (BD Biosciences) and labeled with anti-pe-microbeads
a Beckman Coulter Life Sciences: White Paper
a Beckman Coulter Life Sciences: White Paper An 8-color DuraClone IM panel for detection of Human blood dendritic cells by flow cytometry Nathalie Dupas 1, Snehita Sattiraju 2, Neha Girish 2, Murthy Pendyala
Annexin V-Cy3 Apoptosis Detection Reagent
ab14143 Annexin V-Cy3 Apoptosis Detection Reagent Instructions for Use For the rapid, sensitive and accurate measurement of apoptosis in various samples This product is for research use only and is not
ab Exosome Isolation and Analysis Kit - Flow Cytometry, Cell culture
Version 1 Last updated 14 March 2018 ab228564 Exosome Isolation and Analysis Kit - Flow Cytometry, Cell culture For the measurement of human exosomes in cell culture. This product is for research use only
Instructions. Fuse-It-Color. Overview. Specifications
Membrane fusion is a novel and highly superior method for incorporating various molecules and particles into mammalian cells. Cargo-specific liposomal carriers are able to attach and rapidly fuse with
Thyroid Stimulating Hormone (S-TSH) Thyroid Stimulating
ab108659 Thyroid Stimulating Hormone (S-TSH) Human ELISA Kit Instructions for Use For the quantitative measurement of Human Thyroid Stimulating Hormone (S-TSH) concentrations in serum. This product is
For the rapid, sensitive and accurate measurement of apoptosis in various samples.
ab14082 500X Annexin V-FITC Apoptosis Detection Reagent Instructions for Use For the rapid, sensitive and accurate measurement of apoptosis in various samples. This product is for research use only and
Rapid antigen-specific T cell enrichment (Rapid ARTE)
Direct ex vivo characterization of human antigen-specific CD154+CD4+ T cell Rapid antigen-specific T cell enrichment (Rapid ARTE) Introduction Workflow Antigen (ag)-specific T cells play a central role
NEPHROCHECK Calibration Verification Kit Package Insert
NEPHROCHECK Calibration Verification Kit Package Insert Manufactured for Astute Medical, Inc. 3550 General Atomics Ct. Building 2 San Diego, CA 92121 USA Intended Use The NEPHROCHECK Calibration Verification
ab Cell Invasion Assay (Basement Membrane), 24-well, 8 µm
Version 1 Last updated 29 June 2018 ab235882 Cell Invasion Assay (Basement Membrane), 24-well, 8 µm For the measurement of cell invasion in response to stimuli. This product is for research use only and
Blood Urea Nitrogen Enzymatic Kit Manual Catalog #:
BIOO LIFE SCIENCE PRODUCTS Blood Urea Nitrogen Enzymatic Kit Manual Catalog #: 5602-01 BIOO Scientific Corp. 2010 TABLE OF CONTENTS GENERAL INFORMATION... 1 Product Description... 1 Procedure Overview...
Ex vivo Human Antigen-specific T Cell Proliferation and Degranulation Willemijn Hobo 1, Wieger Norde 1 and Harry Dolstra 2*
Ex vivo Human Antigen-specific T Cell Proliferation and Degranulation Willemijn Hobo 1, Wieger Norde 1 and Harry Dolstra 2* 1 Department of Laboratory Medicine - Laboratory of Hematology, Radboud University
Cell isolation. Spleen and lymph nodes (axillary, inguinal) were removed from mice
Supplementary Methods: Cell isolation. Spleen and lymph nodes (axillary, inguinal) were removed from mice and gently meshed in DMEM containing 10% FBS to prepare for single cell suspensions. CD4 + CD25
For the isolation of mitochondria from P. pastoris and other species of yeast
ab178779 Mitochondrial Yeast Isolation Kit Instructions for Use For the isolation of mitochondria from P. pastoris and other species of yeast This product is for research use only and is not intended for
COMPONENT NAME COMPONENT # QUANTITY STORAGE SHELF LIFE FORMAT. Store at 2-8 C. Do not freeze. Store at 2-8 C. Do not freeze.
This document is available at www.stemcell.com/pis EasySep Mouse Monocyte Isolation Kit Catalog #19861 For processing 1 x 10^9 cells Description Isolate untouched and highly purified monocytes from mouse
Strep-tag HRP Detection Kit (Horse Radish Peroxidase)
Strep-tag HRP Detection Kit (Horse Radish Peroxidase) General protocol for the detection of Strep-tag fusion proteins Last date of revision April 2013 Version PR16-0006 www.strep-tag.com For research use
MagniSort Mouse CD4 Naive T cell Enrichment Kit Catalog Number: RUO: For Research Use Only. Not for use in diagnostic procedures.
Page 1 of 2 MagniSort Mouse CD4 Naive T cell Enrichment Kit RUO: For Research Use Only. Not for use in diagnostic procedures. Mouse splenocytes were unsorted (left) or sorted with the MagniSort Mouse CD4
Nature Immunology: doi: /ni Supplementary Figure 1. Production of cytokines and chemokines after vaginal HSV-2 infection.
Supplementary Figure 1 Production of cytokines and chemokines after vaginal HSV-2 infection. C57BL/6 mice were (a) treated intravaginally with 20 µl of PBS or infected with 6.7x10 4 pfu of HSV-2 in the
ab ORAC Assay Kit
Version 1 Last updated 10 April 2018 ab233473 ORAC Assay Kit For the measurement of ORAC activity in cell lysate, plasma, serum, tissue homogenates and food extracts. This product is for research use only
ROS Activity Assay Kit
ROS Activity Assay Kit Catalog Number KA3841 200 assays Version: 03 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information... 4 Materials
Role of Tyk-2 in Th9 and Th17 cells in allergic asthma
Supplementary File Role of Tyk-2 in Th9 and Th17 cells in allergic asthma Caroline Übel 1*, Anna Graser 1*, Sonja Koch 1, Ralf J. Rieker 2, Hans A. Lehr 3, Mathias Müller 4 and Susetta Finotto 1** 1 Laboratory
20S Proteasome Activity Assay Kit
20S Proteasome Activity Assay Kit For 100 Assays Cat. No. APT280 FOR RESEARCH USE ONLY NOT FOR USE IN DIAGNOSTIC PROCEDURES USA & Canada Phone: +1(800) 437-7500 Fax: +1 (951) 676-9209 Europe +44 (0) 23
Activating and propagating polyclonal gamma delta T cells with broad specificity for
SUPPLEMENTAL MATERIAL: Activating and propagating polyclonal gamma delta T cells with broad specificity for malignancies ADDITIONAL MATERIALS AND METHODS Tumor cell line culture conditions Cell cultures
ab65336 Triglyceride Quantification Assay Kit (Colorimetric/ Fluorometric)
Version 10 Last updated 19 December 2017 ab65336 Triglyceride Quantification Assay Kit (Colorimetric/ Fluorometric) For the measurement of triglycerides in various samples. This product is for research
TECHNICAL BULLETIN. Catalog Number RAB0447 Storage Temperature 20 C
Phospho-Stat3 (ptyr 705 ) and pan-stat3 ELISA Kit for detection of human, mouse, or rat phospho-stat3 (ptyr 705 ) and pan-stat3 in cell and tissue lysates Catalog Number RAB0447 Storage Temperature 20
TECHNICAL BULLETIN. Phospho-Akt (pser 473 ) ELISA Kit for detection of human, mouse, or rat phospho-akt (pser 473 ) in cell and tissue lysates
Phospho-Akt (pser 473 ) ELISA Kit for detection of human, mouse, or rat phospho-akt (pser 473 ) in cell and tissue lysates Catalog Number RAB0011 Storage Temperature 20 C TECHNICAL BULLETIN Product Description
Product information: Human Apolipoprotein B kit 10,000 tests 1. ASSAY DESCRIPTION AND INTENDED USE 2. PROTOCOL AT GLANCE
Headquarters & Europe Office Cisbio Bioassays Phone: +33 ()4 66 79 67 5 Fax: +33 ()4 66 79 19 2 bioassays@cisbio.com USA Office Cisbio US, Inc. Phone: +1 888 963 4567 Fax: +1 781 687 15 htrfinfo@cisbio.us
ab65311 Cytochrome c Releasing Apoptosis Assay Kit
ab65311 Cytochrome c Releasing Apoptosis Assay Kit Instructions for Use For the rapid, sensitive and accurate detection of Cytochrome c translocation from Mitochondria into Cytosol during Apoptosis in
BD CBA on the BD Accuri C6: Bringing Multiplexed Cytokine Detection to the Benchtop
BD CBA on the BD Accuri C6: Bringing Multiplexed Cytokine Detection to the Benchtop Maria Dinkelmann, PhD Senior Marketing Applications Specialist BD Biosciences, Ann Arbor, MI 23-14380-00 Cellular Communication
Supplementalgfigureg1gSchematicgdiagramgofgtumor1modellingg
SChinjectionh F:LuchLCLsh IVhinjectionh T:cellsh Monitorhforhtumorh growthhandhxeno: reactivehgvhd GVLgexperimentg kcbgvsgpbgt1cellse Xeno1reactiveg experimentg kcbgvsgpbgt1cellse IVhinjectionh 5xh,N^6
Naive, memory and regulatory T lymphocytes populations analysis
Naive, memory and regulatory T lymphocytes populations analysis Jaen Olivier, PhD ojaen@beckmancoulter.com Cellular Analysis application specialist Beckman Coulter France Introduction Flow cytometric analysis
ab SREBP-2 Translocation Assay Kit (Cell-Based)
ab133114 SREBP-2 Translocation Assay Kit (Cell-Based) Instructions for Use For analysis of translocation of SREBP-2 into nuclei. This product is for research use only and is not intended for diagnostic
Ge#ng started on the CyTOF: learning by living. Alice Long and BRI HIP core team 2013
Ge#ng started on the CyTOF: learning by living Alice Long and BRI HIP core team 213 BRI Timeline InstallaJon PracJce & Panel Development Nov Dec Jan Feb Mar April May Training Open for business PreparaJon
For the rapid and sensitive extraction of Mammalian proteins from cultured cells and tissue samples.
ab65399 Mammalian Cell Extraction Kit Instructions for Use For the rapid and sensitive extraction of Mammalian proteins from cultured cells and tissue samples. This product is for research use only and
Blocking antibodies and peptides. Rat anti-mouse PD-1 (29F.1A12, rat IgG2a, k), PD-
Supplementary Methods Blocking antibodies and peptides. Rat anti-mouse PD-1 (29F.1A12, rat IgG2a, k), PD- L1 (10F.9G2, rat IgG2b, k), and PD-L2 (3.2, mouse IgG1) have been described (24). Anti-CTLA-4 (clone
A step-by-step approach to build and analyze a multicolor panel
Analyze A step-by-step approach to build and analyze a multicolor panel For Research Use Only. Not for use in diagnostic or therapeutic procedures. Alexa Fluor is a registered trademark of Life Technologies
Mouse serum Insulin 200 tests
Headquarters & Europe Office Cisbio Bioassays Phone: +33 (0)4 66 79 67 05 Fax: +33 (0)4 66 79 19 20 bioassays@cisbio.com cisbio_dd_pi_62in3pef-2µl USA Office Cisbio US, Inc. Phone: +1 888 963 4567 Fax:
ImageStream cytometer analysis. Cells were cultured as described above in vented-cap
ImageStream cytometer analysis. Cells were cultured as described above in vented-cap polypropylene tubes, stained with αcd66b-fitc, αm-dc8-pe and αcd56-pe-cy5.5 mabs, washed and fixed with 4 % (w/v) paraformaldehyde.