E E Hepatitis E SARS 29, Lancet. E A B Enterically-Transmitted Non-A, Hepatitis E. Virus HEV nm. 1.35g/cm s ALT AST HEV HEV

Transcription

1 Hepatitis E Tian-Cheng LI Naokazu TAKEDA Tatsuo MIYAMURA SARS 8 Lancet E E E Hepatitis E VirusHEV E E HEV HEV E ,000 E E A A B Enterically-Transmitted Non-A, Non-B Hepatitis 1983 Balayan A B A IgG 27 30nm 1.35g/cm s ALT AST A B 1990 Reyes cdna E E Department of Virology, II, National Instituite of Infectious Diseases (Gakuen 4-7-1, Musashi-murayama, Tokyo) 6

2 79 HEV water-borne hepatitis virus HEV HEV HEV HCV HBV E HEV HEV HEV 50nm HEV HEV E 30nm 50nm 1991 Tam HEV HEV 4 Genotype 90 Genotype 1G Genotype 2G 2 G E US1 US S1 Genotype 3G U Genotype 4G 4 14 E G3 G HEV 7.2Kb RNA HEV ORF2 7

3 80 5 cap 3 7,200 HEV 3 ORF1ORF3 ORF ,000 ORF1 Y X RNA RNA 3 2,000 ORF2 72 kda ORF3 ORF1 ORF2 IgG IgM RNA RT-PCR IgM E E E HEV HEV RNA HEV E 1920 HEV FRhK42BSA549 HEV HEV E A E HEV HAV E 0 10 AST ALT HEV HEV 7.5kb RNA ORF1 ORF2 ORF3 MT: Y: YPro: PR: X: X H: RdRp: RNA RNA HEV E AST ALT IgM IgG IgM 3 IgG HEVRNA HEVRNA 8

4 81 ALT AST HEV RT-PCR ELISA RT-PCR ORF1 N 500 ORF2 500 RNA HEV RNA RNA HEV ELISA nm Virus-like particlevlp 24 VLP ELISA HEV IgM IgG IgG IgM E 900 IgG ELISA A B C 0 70 A C 29 IgG B 2 IgG 0.42/ IgG A B C

5 82 HEV nm 23.7nm 1.285g/cm 3 100nm E A B C IgG IgM IgG IgM E HEV A B C E HEV % % HEV % E RNA ORF1 ORF2 RT- PCR 3 G3 17 G1 G4 3 G1 87 A B C 11 13% E 15 HEV A B C 1 E 40 ALT AST (1,000 IU/L ) G3 G4 G3 G4 HEV 60% 7% 40% 87% 90% HEV 26 HEV 1.9% 27 HEV IgG 10

6 % 36% 2829 HEV HEV E E DNA DNA Gene gun ORF2 HEV HEV 32 ORF2 56kDa HEV G1 G2G3 HEV 33 56kDa VLP BALB/c IgGIgM IgA 34 IgA HEV HEV VLP IgG 35 VLP 1997 Meng S1 13 E US1 US2 HEV 20 96% 44%36%9% 44 94% 29 62%42 67% 12% IgG 3637 HEV 26.7% 8% 38 HEV HEV HEV 100% 39 HEV HEV HEV 4041 HEV G3 G4 HEV 11

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