Human metapneumovirus:hmpv

Transcription

1 Human metapneumovirus:hmpv Hideaki KIKUTA RS 2001 RS severe acute respiratory syndrome: SARS SARS NL63 HKU1 Human metapneu movirus: hmpv 13.35kb ssrna nm 13 17nm Family ParamyxoviridaeSubfamily PneumovirinaeGenus Metapneumovirus RS respiratory syncytial virus: RSV avian pneumovirus: APV APV 4 A, B, C, DhMPV C RSV hmpv RSV 2 APV 3 NPMFM2SHGL M2 M21 M22 ORF F, G, SH F G Touei Hospital, Pediatric Clinic (3-14, N-41, E-16, Higashi-ku, Sapporo-shi) 7

2 218 RNA N, P, L N nucleoprotein RNA P L phosphoprotein large polymerase protein matrix protein M M2 G, SH, M22 G G RSV 1, 2 A, B AB 12 N, P, M, F, M2 SH, G hmpv hmpv 97 Reverse transcription-polymerase chain reactionrt PCR 73 RNA cdna F, N, L RTPCR RNA PCR hmpv RSV 426 hmpv 8.8 hmpv hMPV hmpv hmpv hmpv hmpv LLCMK hmpv F 8

3 219 RTPCR 1/3 LLCMK2 hmpv IgG RTPCR hmpv 26 IgG IgG IgM hmpv 1 F Tn5 F F F hmpv LLCMK2 F hmpv hmpv hmpv 1016 hmpv hmpv hmpv 9

4 220 hmpv hmpv hmpv hmpv F NK, T hmpv hmpv hmpv F SH, G, M T hmpv F 17 RSV hmpv 1819 RTPCR hmpv X 25 CRP 2021 chronic obstructive hmpv 61 10

5 221 pulmonary disease: COPD hmpv 22 hmpv hmpv RNA hmpv 23 hmpv RSV hmpv RSV hmpv RSV hmpv 24 hmpv RSV hmpv RSV RSV hmpv RSV RSV hmpv RSV hmpv 25 3 ICU hmpv RSV 60ICU RSV 120 hmpv 26 hmpv RSV RSV hmpv IL12, TNFa, IL6, IL1b, IL8, IL10 hmpv RSV hmpv RSV 27 hmpv IFNa RSV RSV hmpv RTPCR hmpv RNA hmpv hmpv RTPCR hmpv hmpv RSVhMPV hmpv RSV RSV RT PCR hmpv RNA van den Hoogen B.G., de Jong J.C., Groen J., et al.: A newly discovered human pneumovirus isolated from young children with respiratory tract disease. Nat Med : , van den Hoogen B.G., Bestebroer T.M., Osterhaus ADME, et al.: Analysis of the genomic sequence of a human metapneumovirus. Virology : , Biacchesi S., Skiadopoulos M.H., Boivin G., et al.: Genetic diversity between human metapneumovirus subgroups. Virology : 1-9, Ishiguro N., Ebihara T., Endo R., et al.: High genetic diversity of the attachment Gprotein of human metapneumovirus. J Clin Microbiol : ,

6 222 Ebihara T., Endo R., Ma X., et al.: Detection of human metapneumovirus antigens in nasopharyngeal secretions by an immunofluorescent antibody test. J Clin Microbiol : , Ebihara T., Endo R., Kikuta H., et al.: Human metapneumovirus infection in Japanese children. J Clin Microbiol : , Ebihara T., Endo R., Kikuta H., et al.: Seroprevalence of human metapneumovirus in Japan. J Med Virol : , Ebihara T., Endo R., Ishiguro N., et al.: Early reinfection with human metapneumovirus in an infant. J Clin Mirobiol : , Ishiguro N., Ebihara T., Endo R., et al.: Immunofluorescence assay for detection of human metapneumovirusspecific antibodies by means of baculovirus-expressed fusion protein. Clin Diag Lab Immunol : , Kuiken T., van den Hoogen B.G., van Riel DAJ, et al.: Experimental human metapneumovirus infection of Cynomolgus Macaques Macaca fascicularisresults in virus replication in ciliated epithelial cells and pneumocytes with associated lesions throughout the respiratory tract. Am J Pathol : , MacPhail M., Schickli JH, Tang R.S., et al.: Identification of small-animal and primate models for evaluation of vaccine candidates for human metapneumovirushmpv and implications for hmpv vaccine design. J Gen Virol : , Alvarez R., Harrod K.S., Shieh W.J., et al.: Human metapneumovirus prsists in BALB/c mice despite the presence of neutralizing antibodies. J Virol : , Hamelin M.-È., Yim K., Kuhn K.H., et al.: Pathogenesis of human metapneumovirus lung infection in BALB/c mice and cotton rats. J Virol : , Tang R.S., Schickli J.H., Mia M., et al.: Effects of human metapneumovirus and respiratory syncytial virus antigen insertion in two 3proximal genome positions of bovine/ human parainfluenza virus type 3 on virus replication and immunogenicity. J Virol : , Biacchesi S., Skiadopoulos M.H., Yang L., et al.: Recombinant human metapneumovirus lacking the small hydrophobic SH and/or attachment G glycoprotein: deletion of G yields a promising vaccine candidate. J Virol : , Buchholz U.J., Biacchesi S., Pham Q.N., et al.: Deletion of M2 gene open reading frames 1 and 2 of human metapneumovirus: Effects on RNA synthesis, attenuation, and immunogenicity. J Virol : , Ma X., Endo R., Ebihara T., et al.: Production and characterization of neutralizing monoclonal antibodies against human metapneumovirus F protein. Hybridoma : , van den Hoogen B.G., van Doornum GJJ, Fockens J.C., et al.: Prevalence and clinical symptoms of human metapneumovirus infection in hospitalized patients. J Infect Dis : , van den Hoogen B.G., Osterhaus D.M., Fouchier R.A.: Clinical impact and diagnosis of human metapneumovirus infection. Pediatr Infect Dis J : S25-S32, Pelletier G., Dery P., Abed Y., et al.: Respiratory tract reinfections by the new human metapneumovirus in an immunocompromised child. Emerg Infect Dis : , Ulloa-Gutierrez R., Skippen P., Synnes A., et al.: Lifethreatening human metapneumovirus pneumonia requiring extracorporeal membrane oxygenation in a preterm infant. Pediatrics : e517-e519, Hamelin M.E., Cote S., Laforge J., et al.: Human metapneumovirus infection in adults with community-acquired pneumonia and exacerbation of chronic obstructive pulmonary disease. Clin Infect Dis : , Schildgen O., Glatzel T., Geikowski T., et al.: Human metapneumovirus RNA in encephalitis patient. Emerg Infcct Dis : , Ebihara T., Endo R., Kikuta H., et al.: Comparison of seroprevalences of human metapneumovirus and human respiratory syncytial virus. J Med Virol : , Greensill J., McNamara P.S., Dove W., et al.: Human metapneumovirus in severe respiratory syncytial virus bonchiolitis. Emerg Infect Dis , Konig B., Konig W., Arnold R., et al.: Prospective study of human metapneumovirus infection in children less than 3 years of age. J Clin Microbiol : , Laham F.R., Israele V., Casellas J.M., et al.: Differential production of inflammatory cytokines in primary infection with human metapneumovirus and with other common respiratory viruses of infancy. J Infect Dis : ,