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3 Ahmed Morad Asaad M.B.B.S, M.Sc., M.D. Professor of Microbiology & Immunology Coordinator of Microbiology Department Scientific Deputy of Prince Meshaal Bin Abdul-Aziz Chair for Endemic Diseases College of Medicine, Najran University Najran Saudi Arabia P.O Box 1988 Phone: ahmedmoradasaad@hotmail amasad@nu.edu.sa

4 Field of Interest: Bacterial infections Molecular antimicrobial drug resistance Mycobacteriology Viral infections Fungal infections Molecular biology techniques Immunological techniques

5 Membership of Societies and Associates: -Saudi Commission for Health Specialties: Consultant Microbiologist Professional Registration -Member of Egyptian Medical Microbiology Society (EMMS). - Editorial Board member in Clinical Microbiology Open Access Journal OMICS - Editorial Board member in Global Scientific Inc Publishing Journals - Editorial Board member in HSOA Journal of Infectious and non Infectious Diseases Herald Scholarly Open Access - Reviewer in many international and national (Egyptian and Saudi) peer-reviewed Journals -Head of Infection Control Committee, Najran University Hospital, Najran, Saudi Arabia

6 Research Under Progress:

7 Research Under Progress: Mohamed AlAyed, Asaad AM, Mohamed Ansar Qureshi, Mohamed Ameen. Viral etiology of respiratory infections in children in Najran, Saudi Arabia, using the multiplex RT-PCR. Saudi Medical Journal (In Press). Jobran Miree Alqahtani, Asaad AM, Essam Mohamed Ahmed, Mohamed Ansar Qureshi. Assessment of bacteriological quality of drinking water in Najran, southwestern Saudi Arabia. Clean SoilAir & Water Journal (In Press). Elabd FM, Zayed MS, Asaad AM, Alseeri S, Qureshi MA and Mussa HA: Molecular characterization of oxacillinases among nosocomial Acinetobacter baumannii isolated from a Saudi hospital. J. Infec. Pub. Health (In Press). Asaad AM, Metawee ME, Zayed MS and Qureshi MA. Distribution and implications of Hepatitis B Virus (HBV) genotypes in Saudi patients with different HBV clinical presentations in Najran Region. (Under progress)

8 Publications:

9 Alqahtani JM, Abu-Eshy SA, Mahfouz AA, El-Mekki AA and Asaad AM: Seroprevalence of hepatitis B and C virus infections among health students and health care workers in the Najran region, southwestern Saudi Arabia: The need for national guidelines for health students. BMC Public Health 2014; 14: Alqahtani JM and Asaad AM: Anti-tuberculous drugs susceptibility testing methods: Current knowledge and future challenges. Mycobacterial Diseases Journal 2014, 4: doi: / Asaad AM, Zaid MS, Qureshi MA: Emergence of unusual nonfermenting gram negative nosocomial pathogens in a Saudi hospital. Japanese Journal of Infectious Diseases 2013; 66: Asaad AM, Qureshi MA: Increased vancomycin minimum inhibitory concentrations of methicillin-resistant Staphylococcus aureus nosocomial isolates from southwestern Saudi Arabia. American Journal of Epidemiology and Infectious Diseases 2013; 1:

10 Asaad AM, Jobran Miree Alqahtani: Primary anti-tuberculous drugs resistance of pulmonary tuberculosis in Southwestern Saudi Arabia. 3 rd International Conference and Exhibition, 2013 on Pharmaceutics & Novel Drug Delivery Systems, Chicago/Northbrook, USA. Mahmoud MM, Asaad AM, Qureshi AM: Hand rubbing and scrubbing in relation to microbial count among surgical team members in a Saudi Hospital. Life Science Journal 2013; 10: Mahmoud MM, Asaad AM: SURGICAL ASEPSIS PRACTICES AMONG OR STAFF IN KING KHALID HOSPITAL, NAJRAN. International Journal of Current Research 2013; 5: Zaid MS, Asaad AM, Alhayek AA, Qureshi MA: Etiology of acute gastroenteritis in children in Najran Saudi Arabia. Journal of Health Specialities 2013; 1: Asaad AM, Alqahtani JM: Primary antituberculous drugs resistance of Pulmonary tuberculosis in southwestern Saudi Arabia. Journal of Infection and Public Health 2012; 5:

11 Asaad AM, Alqahtani JM: Serological and molecular diagnosis of human brucellosis in Najran, Saudi Arabia. Journal of Infection and Public Health 2012; 5: Asaad AM, Al Yousef S, Al gharawi A: Metallo-β-Lactamase producing Pseudomonas aeruginosa clinical isolates from a Saudi Military Hospital. Egy. J. Med. Microbiol. 2009; Vol. 18, No. 3: Howyda MI, Asaad AM and Ahmed Amer: Antifungal susceptibility patterns of Dermatophytes clinical isolates from dermatophytosis patients before and after therapy. Egy. J. Med. Microbiol. 2010; Vol. 19, No. 1: Ahmed AS, Asaad AM, Amal HA and Azza OA: Phenotypic and genotypic patterns of aminoglycosides-resistant Staphylococcus aureus clinical isolates. Egy. J. Med. Microbiol. 2010; Vol. 19, No. 2: 1-8. Ahmed SS, Abeer MN, Asaad AM and Ayman AA: Clinical and microbiological features of nosocomial infections caused by Acinetobacter species. Egy. J. Med. Microbiol. 2010, Vol. 19, No. 2:

12 Abeer M. Nafee and Asaad AM: Antimicrobial resistance trends and distribution of virulence genes among Shigella isolates from patients with acute diarrhea. J. Trop. Med. 2010; Vol. 11, No. 4. Asaad AM, Jehan A El-Shennawy, Howyda M Ebrahim and Hala M Morsi: Staphylococcus aureus isolates from patients with atopic dermatitis: Clinical, bacteriological and molecular characters. Egy. J. Med. Microbiol. 2010; Vol. 19, No. 3: Jehan A El-Shennawy and Asaad AM: Comparative study of different methods to detect primary and non primary cytomegaloviral infection in pregnant women. Egy. J. Med. Microbiol. 2010; Vol. 19, No. 4: Neveen H.A, Hanan MT and Asaad AM: Control of Listeria monocytogenes and Staphylococcus aureus isolated from chicken meat and chicken products by dipping in some organic acid solutions. Bulletin of Animal Health and production in Africa 2008; Vol. 56, No. 4:

13 Asaad AM, Al Yousef S: Surveillance of antimicrobial resistance in a Military Hospital, Saudi Arabia using WHONET 5 program. Egy. J. Med. Microbiol. 2007; Vol. 16, No. 1: Asaad AM. WHONET: What is it? NAAFH CPE Bulletin, 2007; vol. 8, No. 5: 5. Asaad AM. Lecture Notes on Microbiology. Nursing Diploma Program Book, Northern Area Armed Forces Hospital, King Khalid Military City, Saudi Arabia. Asaad AM, Al-Bialy AA and Awad-Alla MG. Bacteriological and molecular aspects of Staphylococcus aureus clinical isolates carrying genes for exfoliative toxins and Panton-Valentine leukocidin. Egy. J. Med. Microbiol. 2004; Vol. 11, No. 1. Asaad AM, Amer A and Abd El-Dayem WA. Performance of enzyme immunoassay and PCR for rapid diagnosis of Helicobacter pylori infection. Egy. J. Med. Microbiol. 2003; Vol. 12, No. 2.

14 Asaad AM, El-Behedy EM and Amer A. Diagnosis of Shigella infection in children with acute diarrhea by conventional and molecular methods. Egy. J. Med. Microbiol. 2003; Vol. 12, No. 1. Tema MA, Asaad AM, Mamoon M. Use of PCR for diagnosis and follow up of leprosy patients during multi-drug therapy. Egy. J. Med. Microbiol. 2003; Vol. 9, No. 2. Elshamy EA and Asaad AM. Some pro-inflammatory and antiinflammatory cytokines in cirrhotic ascetic patients with and without spontaneous bacterial peritonitis. Egy. J. Med. Microbiol. 2003; Vol. 9, No. 3. Hasanen OM, Asaad AM and Allam A. Prevalence of TT virus infection in Egyptian children and adults and in patients with liver disease. Egyp. J. Intern Med 2002; vol. 14, No.4.

15 Abd El-Dayem WA, Sherben AS, Elshami EA and Asaad AM. Antineutrophil cytoplasmic antibodies types and titers in ulcerative colitis patients. Relationship with disease variables. Zag Univ Med J 2002, vol. 8, No. 7. Asaad AM, Abo-Taleb AF and Mahfouz T. Susceptibility testing of Mycobacterium tuberculosis in clinical isolates by three different methods. Egy. J. Med. Microbiol. 2001; Vol. 10, No. 4.

16 Our ongoing research project Distribution and implications of Hepatitis B Virus (HBV) genotypes in Saudi patients with different HBV clinical presentations in Najran Region Hepatitis B virus (HBV) infection remains a serious health problem. According to the homogeneity of virus sequences, at least 10 HBV genotypes (A to J) have been defined throughout the world with a characteristic geographical distribution. Several HBV genotyping methods are in widespread use, such as direct sequencing, restriction fragment length polymorphism analysis, reverse hybridization, and enzyme-linked immunosorbent assays. Within the last few years, a well standardized multiplex nested PCR protocol was developed for simultaneous detection of HBV load and identification of HBV genotypes A to G.

17 The knowledge regarding clinical significance of HBV genotypes in terms of clinical outcomes and therapeutic response to antiviral therapy in patients with HBV related severe liver conditions is still very limited and results from different research groups were discordant. In Saudi Arabia, although HBV is hyperendemic, little is known about the prevalence and distribution of HBV genotypes. Furthermore, the association between the distinct genotypes and the severity of liver disease in the country remains unreported. To our knowledge, this study will be the first to determine the prevalence and distribution of HBV genotypes in HBV patients using multiplex nested PCR protocol and investigate the implications of HBV genotypes in HBV patients with different clinical presentation.

18 This study aims to determine the prevalence and distribution of HBV genotypes in HBV patients and investigate the implications of HBV genotypes in HBV patients with different clinical presentation. Research Methodology: Design The study will be a cross-sectional study on a representative sample of HBV patients attending Najran University Hospital, Maternity and Children Hospital and King Khalid Hospital, Najran, Southwestern Saudi Arabia. Clinical assessment of cases The study will include 100 Saudi patients with HBV infection. They will be subjected to:

19 Clinical assessment Liver function tests (billirubin, AST and ALT) HBV markers (HBs Ag, HBe Ag, Anti-HBs Ab, Anti-HBe Ab) α-fetoprotein The patients will be classified into 3 groups on the basis of clinical and laboratory findings: Group 1 (acute HBV) Group 2 (chronic HBV) Group 3 (HBV patients with hepatocellular carcinoma) Blood samples will be collected from all patients for molecular analysis.

20 Processing and handling of clinical specimens All samples will be subjected for DNA extraction by a commercial kit from reputed manufacturers. Samples will be frozen at -20⁰C for further analysis. Multiplex nested PCR DNA amplification by multiplex nested PCR using well defined sequence primers in two tubes: The first tube for detecting HBV genotypes A, B or C The second tube for detecting HBV genotypes D, E, F or G)

21 Clinical Microbiology-Open Access Bacteriology & Parasitology Clinical Microbiology: Open Access Virology & Antiviral Research Virology & Mycology

22 Clinical Microbiology-Open Access 2nd International Congress on Bacteriology and Infectious Diseases 3rd International Conference on Clinical Microbiology & Microbial Genomics

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