PRODUCTS CATALOG OUR MISSION:

Transcription

1 PRODUCTS CATALOG OUR MISSION: TO SAVE LIVES AND IMPROVE HEALTH BY EFFECTIVELY RESPONDING TO OUR CHANGING WORLD WITH INNOVATIVE VACCINES AND BIOPHARMACEUTICALS

2 TABLE OF CONTENTS 3 Influenza Research Antigens 4 Influenza Antibodies 5 HIV & SIV Research Antigens 6 HIV-1 gp120 LAV, MN, CM 7 HIV-1 gp160 LAV & MN 8 HIV-1 p55 gag 9 HIV-1 p66 10 HIV-1 p24 gag 11 SIV-1 p55 12 Other Proteins 12 Recombinant EPO 13 Recombinant Human CEA 14 Recombinant Human CD4 15 Recombinant TSP 16 Ordering 16 Terms, Conditions, Policies 2 PROTEIN SCIENCES PRODUCTS CATALOG

3 Influenza Research Antigens (rha) We are the world leader in recombinant influenza hemagglutinin (rha) production. We have more than 15 years of experience producing full-length rha for research, vaccine and diagnostic purposes. We maintain a variety of rha subtypes in inventory. These strains are provided individually in solution in 100 µg and 1 mg vial sizes. Please inquire about additional rha strains that are available by special order. Catalog number 3006 (include the subtype being ordered) Purified under conditions that maintain the oligomeric structure of the protein Tested for sterility and pyrogenicity Provided with Certificate of Analysis Shipped on ice packs. Store at 2º C to 8º C. ASSAY Western blot ELISA PROTEIN REQUIRED µg/strip 1.0 µg/well Recombinant HA proteins are provided only for use in research or vitro testing. They are not for use in humans or for clinical diagnostic procedures and any such use is unlawful. Please contact sales*@proteinsciences.com (delete * to send ) for information regarding protein for diagnostic procedures. Strains Offered: H1 A/Beijing/262/1995 A/Brisbane/59/2007 A/Chile/01/1983 A/California/04/2009 A/California/07/2009 A/Michigan/45/2015 A/New Caledonia/20/1999 A/Puerto Rico/08/1934 A/Solomon Islands/03/2006 A/Taiwan/01/1986 A/Texas/36/1991 H2 A/Ann Arbor/6/1960 A/Singapore/01/1957 H3 A/Alaska/18/2014 A/Brisbane/10/2007 A/Duck/Shan Tou/1283/2001 A/Fujian/411/2002 A/Hong Kong/1/1968 A/Hong Kong/4801/2014 A/Indiana/10/2011 A/Indiana/07/2012 A/Indiana/12/2012 A/Johannesburg/33/1994 A/Minnesota/11/2010 A/Mississippi/1/1985 A/Nanchang/933/1995 A/New Caledonia/71/2014 A/New York/39/2012 A/New York/55/2004 A/Norway/3806/2016 A/Panama/2007/99 A/Perth/16/2009 A/Port Chalmers/1/1973 A/Shangdong/9/1993 A/Sichuan/2/1987 A/Switzerland/ /2013 A/Sydney/5/1997 A/Texas/1/1977 A/Texas/50/2012 A/Uruguay/716/2007 A/Victoria/210/2009 A/Victoria/361/2011 A/Wisconsin/67/2005 A/Wyoming/3/2003 H5 A/Anhui/01/2005 A/Bar Headed Goose/1a/2005 A/Hong Kong/156/1997 A/Indonesia/05/2005 A/Vietnam/1203/2004 A/Gyrfalcon/Washington/ /2014 H6 A/Teal Hong Kong/W312/1997 H7 A/Anhui/1/2013 A/Canada/RV444/2004 A/Guangdong/17SF003/2016 A/Hong Kong/125/2017 A/Netherlands/219/2003 H9 A/Chicken/HongKong/G9/1997 A/Hong Kong/1073/1999 H10 A/Harbor Seal/Germany B Strains B/Brisbane/60/2008 B/Hong Kong/330/2001 B/Jiangsu/10/2003 B/Malaysia/2506/2004 B/Ohio/01/2005 B/Wisconsin/01/2010 Zika Zika E protein, ectodomain B/Florida/04/2006 B/Hubei/158/2009 B/Jilin/20/2003 B/Massachusetts/02/2012 B/Phuket/3073/2013 PROTEIN SCIENCES PRODUCTS CATALOG 3

4 Influenza Antibodies We have added polyclonal antibodies generated against three of our purified rha strains to the product line. These antibodies were produced by immunization of rabbits with purified recombinant hemagglutinin from either H1 A/New Caledonia/20/99, H3 A/Wisconsin/67/05, or B/Jiangsu/10/03. These antibodies are provided individually in solution in 200 µg vial sizes. ANTI-H1 INFLUENZA HEMAGGLUTININ RABBIT POLYCLONAL Catalog number 6000 ANTI-H3 INFLUENZA HEMAGGLUTININ RABBIT POLYCLONAL Catalog number 6001 ANTI-HB INFLUENZA HEMAGGLUTININ RABBIT POLYCLONAL Catalog number 6002 Anti-HA polyclonal antibodies are provided only for research use or in vitro testing. They are not for use in humans or for clinical diagnostic procedures and any such use is unlawful. Please contact (delete * to send ) for information regarding protein for diagnostic procedures. Purified under conditions that maintain the activity of the antibody Tested for sterility and pyrogenicity Provided with Certificate of Analysis Shipped on ice packs. Store at 2º C to 8º C. ASSAY Western blot ELISA PROTEIN REQUIRED µg/strip To be determined 4 PROTEIN SCIENCES PRODUCTS CATALOG

5 HIV & SIV Research Antigens We offer a variety of research proteins for the study of human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV). These products are intended for use in laboratory animals or in vitro testing only and may not be used in humans or for clinical diagnostic procedures, and any such use is unlawful. Proteins are typically supplied in buffered saline and provided in 100 µg vials, and are tested for sterility and absence of pyrogens. HIV-1 gp120 Full-length, glycosylated gp120. Proteins from the LAV, MN & CM strains are available. HIV-1 p55 HIV-1 gag derived from the HTLV-III/LAV isolate (clade B). HIV-1 p24 gag The capsid protein derived from the NY5 strain. HIV-1 gp160 Tetrameric, glycosylated env protein from the LAV & MN strain; produced as the full-length gp160 precursor in insect cells. HIV-1 p66 Reverse Transcriptase The pol protein derived from the LAV strain. SIV-1 p55 SIV-1 gag derived from SIV mac 23g and SIV smh4 isolates. PROTEIN SCIENCES PRODUCTS CATALOG 5

6 HIV-1 gp120 LAV, MN, CM Catalog number 2003LAV (HIV-1); 2003MN; 2003CM Recombinant HIV-1 gp120 is produced in insect cells, as was the gp120 whose crystal structure was reported in: Nature 1998 Jun 18; 393(6686): LAV (IIIB), MN, CM Produced using recombinant baculovirus vectors Secreted from insect cells in serum-free media Purified under non-denaturing conditions Full length and glycosylated Supplied in buffered saline (no carrier proteins; no preservative) Tested for sterility and pyrogens 100 µg/ml Shipped on dry ice (frozen). Store at -20º C. Avoid freeze-thawing. Recombinant HIV proteins are provided only for research use or in vitro testing. They may not be used in humans or for clinical diagnostic procedures and any such use is unlawful. 10 mm Tris, ph mm NaCl 0.01% Triton N PROTEIN SCIENCES PRODUCTS CATALOG

7 HIV-1 gp160 LAV & MN Catalog number 2000LAV; 2000MN Recombinant HIV-1 gp160 LAV (IIIB) and MN Produced using recombinant baculovirus vectors in insect cells Full-length, tetrameric, and glycosylated with N-linked sugars Purified under conditions that maintain the oligomeric structure of the molecule Supplied in buffered saline (no carrier proteins; no preservative) Tested for sterility and pyrogens 100 µg/ml Shipped on ice packs / dry ice. Store at 2º C to 8º C. Recombinant HIV proteins are provided only for research use or in vitro testing. They may not be used in humans or for clinical diagnostic procedures and any such use is unlawful. 10 mm Tris, ph mm NaCl 0.01% Tween-20 HIV & SIV RESEARCH ANYIGENS PROTEIN SCIENCES PRODUCTS CATALOG 7

8 HIV-1 p55 gag Catalog number 2005 Recombinant HIV-1 p55 gag gene product HTLV-III/LAV isolate Produced using baculovirus vectors in insect cells Full-length authentic p55 gag protein is produced Forms approximately 23 nm particles Supplied in buffered saline with 0.4 M arginine Tested for sterility and pyrogens 100 µg/ml Shipped on dry ice (frozen). Store at -20º C. Avoid freeze-thawing. Recombinant HIV proteins are provided only for research use or in vitro testing. They may not be used in humans or for clinical diagnostic procedures and any such use is unlawful. 10 mm Tris, ph mm NaCl 400 mm L-Arginine 8 PROTEIN SCIENCES PRODUCTS CATALOG

9 HIV-1 p66 Catalog number 2008 Recombinant HIV-1 p66 pol gene product (reverse transcriptase) LAV (IIIB) isolate Produced using baculovirus vectors in insect cells Molecular weight approximately 66,000 Supplied in buffered saline (no carrier proteins; no preservative) Tested for sterility and pyrogens 100 µg/ml Functional activity and use Immunoreactive with sera from HIV-infected individuals Induces type-specific neutralizing antibodies in experimental animals Western blots: µg/strip ELISA: µg/well Shipped on dry ice (frozen). Store at -20º C. Avoid freeze-thawing. Recombinant HIV proteins are provided only for research use or in vitro testing. They may not be used in humans or for clinical diagnostic procedures and any such use is unlawful. 30 mm bis-tris, ph mm NaCl 0.2 mm EDTA 2 mm ß - mercaptoethanol HIV & SIV RESEARCH ANYIGENS PROTEIN SCIENCES PRODUCTS CATALOG 9

10 HIV-1 p24 gag Catalog number 2004 Recombinant HIV-1 p24 gag gene product NY5 isolate Produced using recombinant baculovirus vectors in insect cells Full-length plus 12 amino acids of the C-terminus of p17 and 57 amino acids of the N-terminus of p15 Supplied in buffered saline (no carrier proteins; no preservative) Tested for sterility and pyrogens 100 µg/ml Shipped on dry ice (frozen). Store at -20º C. Avoid freeze-thawing. Recombinant HIV proteins are provided only for research use or in vitro testing. They may not be used in humans or for clinical diagnostic procedures and any such use is unlawful. 10 mm Tris, ph mm NaCl 0.01% Tween PROTEIN SCIENCES PRODUCTS CATALOG

11 SIV-1 p55 gag Catalog number 3005 Strains: SIV mac 239; SIV smh4 Recombinant SIV-1 p55 gag Produced using baculovirus vectors Secreted from insect cells in serum-free media Shipped on dry ice. Store at -20º C. Recombinant HIV proteins are provided only for research use or in vitro testing. They may not be used in humans or for clinical diagnostic procedures and any such use is unlawful. 10 mm Tris, ph mm NaCl 400 mm L-Arginine HIV & SIV RESEARCH ANYIGENS PROTEIN SCIENCES PRODUCTS CATALOG 11

12 Other Proteins We offer the following products in addition to our influenza, HIV amd SIV product line. These products are intended for use in laboratory animals or in vitro testing only and may not be used in humans or for clinical diagnostic procedures, and any such use is unlawful. Proteins are typically supplied in buffered saline at a concentration of 100 µg/ ml and are tested for sterility and absence of pyrogens. repo (Human Erythropoietin) Glycoprotein, high purity, recombinant EPO rcd4 (Human Soluble CD4) High purity, recombinant, biologically active soluble CD4 rcea (Human Carcinoembryonic Antigen) High purity, recombinant, soluble CEA rtsp (Human Thrombospondin) Recombinant secretory product of thrombinstimulated platelets repo Catalog number 3004 Recombinant human EPO Soluble glycosylated form Produced using baculovirus vectors Secreted from insect cells in serum-free media Approximately 28,000 molecular weight Shipped on ice packs. Store at 2º C to 8º C. Recombinant EPO proteins are provided only for research use or in vitro testing. They may not be used in humans or for clinical diagnostic procedures and any such use is unlawful. 10 mm NaH 2 PO 4, ph mm NaCl 12 PROTEIN SCIENCES PRODUCTS CATALOG

13 rcea Catalog number 3000 Recombinant human CEA Tumor antigen found associated with colon, breast and lung cancer cells Produced using recombinant baculovirus vectors in insect cells Full-length with the same amino acid sequence as mature tumor-driven human CEA Glycosylated with N-linked sugar Approximately 120,000 molecular weight Supplied in buffered saline (no carrier proteins; no preservative) Tested for sterility and pyrogens Shipped on dry ice (frozen). Store at -20º C. Avoid freeze-thawing. Recombinant CEA is provided only for research use or in vitro testing. It may not be used in humans or for clinical diagnostic procedures and any such use is unlawful. 10 mm NaH 2 PO 4, ph mm NaCl OTHER PROTEINS PROTEIN SCIENCES PRODUCTS CATALOG 13

14 rcd4 Catalog number 3002 Recombinant human soluble CD4 Produced using recombinant baculovirus vectors Secreted from insect cells in serum-free medium Purified under non-denaturing conditions Full-length and glycosylated (ectodomain only) Supplied in buffered saline (no carrier proteins; no preservative) Tested for sterility and pyrogens 100 µg/ml Shipped on dry ice (frozen). Store at -20º C. Avoid freeze-thawing. Recombinant CD4 is provided only for research use or in vitro testing. It may not be used in humans or for clinical diagnostic procedures and any such use is unlawful. 10 mm NaH 2 PO 4, ph mm NaCl 0.01% Tween PROTEIN SCIENCES PRODUCTS CATALOG

15 rtsp Catalog number 3003 Recombinant human TSP Inhibitor of new blood vessel formation (angiogenesis) Produced using baculovirus vectors in insect cells Reactive with A4.1 anti-tspmab Glycosylated with N-linked sugars Approximately 140,000 molecular weight Shipped on ice packs. Store at -8º C to -2º C. Recombinant TSP is provided only for research use or in vitro testing. It may not be used in humans or for clinical diagnostic procedures and any such use is unlawful. 30 mm NaH 2 PO 4, ph mm NaCl OTHER PROTEINS PROTEIN SCIENCES PRODUCTS CATALOG 15

16 ORDERING RESEARCH PRODUCTS We offer a variety of purified recombinant proteins for the study of human and veterinary diseases, including our proprietary research antigens derived from many different influenza strains. All of our proteins are produced using our BEVS technology in expressf+ insect cells. We also offer several polyclonal antibodies for detection of specific hemagglutinin antigens. PRODUCT ORDER TERMS, CONDITIONS, POLICIES Terms Net 30 days after shipment, in U.S. dollars. Payment may be made by wire transfer, credit card or check. Prices are subject to change without notice. Payment address: Protein Sciences, c/o People s United Bank, P.O. Box 14, Brattleboro, VT Conditions Products are sold for research or laboratory use only and are not to be administered to humans nor used in clinical diagnostic procedures. Delivery orders will be shipped on ice by air express. Unless otherwise specified, products are shipped F.O.B. origin, with freight charges prepaid and added to your invoice. Shipping and Handling Per order: $50 (domestic), $200 (international). Note that for all international orders, import taxes will be prepaid by Protein Sciences when required and invoiced separately to the customer with an additional $20 administration fee. Shipments to Europe will only occur on Mondays and Fridays. Limited Product Warranty Any product not meeting performance standards stated in the product specification sheet will be replaced at no charge. This warranty limits our liability to the replacement of the product. No other warranties of any kind, express or implied, arising by law or otherwise, including without limitation implied warranties of merchantability or fitness for a particular purpose, are provided by Protein Sciences. Protein Sciences shall have no liability for any direct, indirect, consequential or incidental damages arising from the use, the results of the use or the inability to use any product. Sales and Technical Support For sales and technical support, please our Manager of Research Products. To order research antigens online, click HERE. F For reagent orders or inquiries from Japan, click HERE. 16 PROTEIN SCIENCES PRODUCTS CATALOG

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