Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK

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1 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Department of Microbiology Princess Alexandra Hospital Hamstel Road Harlow CM20 1QX Contact: D Orriss Tel: Debbie.Orriss@pah.nhs.uk Website: Testing performed at the above address only DETAIL OF ACCREDITATION Swabs (throat, ear, nose, wound, eye), CSF, genital tract specimens, sterile fluids, tissues, sputum and respiratory samples, faeces, paediatric urine (on-call) bacteria and fungi of clinical significance. Using manual culture, staining and microscopy LP SOP for examination of CSF LP SOP for isolation of H.pylori from gastric biopsy LP SOP for neonatal screening samples LP SOP for examination of respiratory and associated samples LP SOP for examination of faeces LP SOP for examination of urine LP SOP for Gram stain LP SOP for examination of genital tract and associated samples LP SOP for examination of fluids normally sterile LP SOP for detection of STD in samples from sexual health clinic LP SOP for examination of wound and associated samples LP SOP for the examination of nose, throat and eye swabs LP SOP for investigation of tissues and biopsies Assessment Manager: FN Page 1 of 5

2 Nose, throat, groin, wounds, urine and sputum Whole blood Sputum, tissue, fluids Clinical isolates cultured in-house MRSA bacteria Isolation of bacteria and fungi of clinical significance. mycobacteria of clinical significance. Identification of microorganisms of clinical significance. Detection and quantification of white cells, red blood cells and bacteria Qualitative detection of Legionella pneumophila serogroup 1 antigen Using manual chromogenic culture, staining/microscopy SOP: LP Using Becton Dickinson BacTalert SOP : LP Using Becton Dickinson MGIT, manual auramine staining, microscopy. SOP: LP Using manual biochemical tests and Bruker Maldi-TOF SOP: LP Using Beckman Coulter IQ2000 Sprint. SOP: LP Proflow Legionella test kit by lateral flow immunoassay SOP: LP (Legionella) Qualitative detection of S. pneumoniae antigen Alere BinaxNOW Streptococcus pneumoniae Antigen Card by lateral flow immunoassay LP (s.pneumoniae) Qualitative detection of Helicobacter pylori antigen Proflow H. pylori test Lateral flow immunoassay SOP: LP Detection of C.difficile toxins A & B Alere Tox A/B Quik Chek kit Lateral flow immunoassay SOP LP Detection of Rotavirus antigen Meridian Bioscience Immunocard Rapid Immunochromatographic Assay STAT kit SOP LP Assessment Manager: FN Page 2 of 5

3 CSF and ascetic fluid Hair, skins, nails Cell count for red blood cells and/or white blood cells Mycology examination activities for Isolation and examination of superficial mycoses/dermatophytes of clinical significance Parasitology examination activities Detection and identification of helminths, nematodes, flukes, parasitic larvae, protozoa, ova, eggs, and cysts of clinical significance Detection and identification cyclospora and cryptosporidium Manual methods using Neubauer counting chamber and microscopy SOP LP Microscopy performed using fluorescent microscope. Manual culture using selective agars SOP: LP Using Parasep faecal concentrator kit and microscopy LP SOP for examination of faeces Using manual staining and microscopy LP SOP for Modified ZN stain for Cyclospora LP SOP for auramine phenol stain for Cryptosporidium Assessment Manager: FN Page 3 of 5

4 Genital swabs Molecular examination activities for Chlamydia and N. gonorrheae nucleic acid testing. Automated nucleic acid extraction and PCR amplification using COBAS 4800 SOP: LP Seeprep12 (Werfen) using magnetic bead nucleic acid extraction. Smart Cycler (Cepheid) using reverse transcription realtime PCR Genital swabs N. gonorrheae confirmatory testing SOP: LP Genital swabs Herpes Simplex virus testing SOP: LP Detection and identification of norovirus DNA Serology examination activities for Detection of C. difficile Glutamate Dehydrogenase SOPs LP LP Biomerieux VIDAS 2-step sandwich immunoassay SOP LP Serum/plasma Detection of the following: VIDAS (Biomerieux) using enzyme immunoassay with fluorescent detection (ELFA) VZ IgG LP SOP for detection of Varicella-Zoster IgG Measles IgG LP SOP for detection of Measles IgG CMV IgG LP SOP for detection of CMV IgG Rubella IgM LP SOP for detection of Rubella IgM Assessment Manager: FN Page 4 of 5

5 Serology examination activities for Mumps IgG Hepatitis B Core antibodies Toxoplasma antibodies Hepatitis A IgM Hepatitis A total antibody LP SOP for detection of Mumps IgG LP SOP for detection of Hepatitis B core antibodies LP b SOP for detection of Toxoplasma antibodies by VIDAS LP SOP for detection of Hepatitis A IgM LP SOP for detection of Hepatitis A total antibody Serum/plasma Detection of the following Architect i2000 (immuno platform) using chemiluminescent immunoassay HIV (antigen/antibody combo) LP SOP for detection of HIV antigen and antibody LP SOP for detection of Hepatitis B antibodies LP SOP for detection of Hepatitis C antibodies LP SOP for detection of Hepatitis C antibodies Rubella IgG LP SOP for detection of Rubella IgG Syphilis antibodies LP SOP for detection of Syphilis antibodies CMV IgM LP SOP for detection of CMV IgM Serum/plasma Detection of Anti- Streptolysin O Manual Latex agglutination method.rheumajet ASO, Werfen SOP LP Miscellaneous examination activities for the purpose of clinical diagnosis Detection of urinary human chorionic gonadotropin END In house documented methods Using Invitech pregnancy test kit, cartridge based double antibody sandwich immunoassay SOP: LP Assessment Manager: FN Page 5 of 5