TEST REQUEST INFORMATION- VIROLOGY
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1 Chlamydia/ Gonorrhea Nucleic Acid Amplification VC75 Qualitative Nucleic Acid Amplification SPECIMEN: Genital swab or first catch urine CONTAINER:GEN-PROBE APTIMA 2 Combo swab transport tube or urine transport tube. COLLECTION: See virology specimen collection guide Temperature: 2-30 degrees C acceptable, within (urines) 30 days, (swabs) 60 days Refrigerated: 2-8 degrees C prefered, within (urine) 30 days, (swabs) 60 days NORMAL VALUE TEST METHOD Genprobe, Aptima 2 CPT CODES Hepatitis B, Carrier Screen (Hepatitis B Surface antigen) VS27 Enzyme Immunoassay HBsAg CONTAINER: Serum Separator Tube ( SST), (1 Auszyme, Hepatitis B, Immunity (Past case) (Hepatitis B Core Ab) VS29 Enzyme Immunoassay HBcAb total CONTAINER: Serum Separator Tube ( SST), (1 Corzyme, Hepatitis B, Immunity, or vaccinated (Anti-HBsAg) VS28 Enzyme Immunoassay Anti-HBsAg Ausab EIA, 86706
2 Hepatitis C Ab Total VS66 Enzyme Immunoassay Hepatitis Screen- Acute, includes Hepatitis A IgM Hepatitis B surface Ag Hepatitis B Core IgM VS26 VS67 VS62 Microparticle Enzyme Immunoassay NORMAL TEST VALUE METHOD HCV, EIA 2.0, HAVAB-M HBsAg Core-M, CPT CODES HIV-1 antibody, confirmation IFA included with antibody screen if required Indirect Fluorescent Antibody HIV-1 Fluorognost HIV-1 IFA Walheim HIV-1 antibody, confirmation included with antibody screen if required Western Blot Frozen:-20 C,serum only HIV-1 Cambridge Biotech HIV-1 Western Blot Kit, Calypte Biomedical 86689
3 HIV-1 Antibody, Oral Fluid HIV-1 Viral Load (Standard) VH74 VH72 Enzyme Immunoassay. Sent to Los Angeles Public Health Laboratory for testing Quantitative RT-PCR; Test Range; <400 to >750,000 copies/ml SPECIMEN: Oral fluid CONTAINER: Orasure oral fluid collection vial. COLLECTION: See serology specimens collection guide., 21 days, 21 days Frozen unacceptable SPECIMEN: Plasma, 1ml (min. vol. 0.3ml) CONTAINER: Vacutainer Lavender Top (EDTA) COLLECTION: Aseptically collect 3ml of blood in Lavender Top (EDTA) tube. Draw approximately 2.5 times the volume of whole blood as the volume of plasma required. CONDITIONS: Refrigerated: Transport to lab within 6 hours. Frozen: Transport on dry ice, must arrive at lab frozen. Separated plasma only. NORMAL TEST CPT VALUE METHOD CODES Send Out Not Detected, <400 Amplicor HIV- 1 Monitor Roche HIV-1 Viral Load (Ultra-Sensitive) VH72A Quantitative RT-PCR: Test SPECIMEN: Plasma, 1ml (min. vol. 0.6ml) range <50 to >75,000 copies/ml CONTAINER: Vacutainer Lavender Top (EDTA) COLLECTION: Aseptically collect 3ml of blood in Lavender Top (EDTA) tube. Draw approximately 2.5 times the volume of whole blood as the volume of plasma required. CONDITIONS: Refrigerated: Transport to lab within 6 hours. Frozen: Transport on dry ice, must arrive at lab frozen. Separated plasma only Not Detected, <50 Amplicor HIV- 1 Monitor Roche HIV-1/HIV-2 Antibody, screen, includes confirmation if required (IFA or Western Blot) VH31 Enzyme Immunoassay HIVAB HIV- 1/HIV-2, 86701
4 Measles (Rubeola), IgG Antibody (Immune status) VS17 Indirect Fluorescent Antibody, IgG NORMAL VALUE TEST METHOD Measles-G Test System, Bion CPT CODES Measles (Rubeola), IgM Antibody (active case) VS17 Indirect Fluorescent Antibody, IgM Measles-M Test System, Bion Rubella IgG Antibody (Immune status) VS24 Microparticle Enzyme Immunoassay IgG Rubella IgG 2.0, Rubella IgM Antibody (active case) VS63 Microparticle Enzyme Immunoassay IgM CONTAINER: Serum Separator Tube ( SST), 1 Rubella IgM, Viral Culture, Other (Specify virus suspected) VI22 Tissue Culture Isolation, Identification Dependant upon patiient symptoms and virus suspected, call laboratory for assistance, see virology specimen collection guide, Culture 87252
5 Viral Culture ID VI22A Tissue Culture Isolation, Identification SPECIMEN: Infected tissue culture. CONTAINER: Tissue culture tube. COLLECTION: Not applicable CONDITIONS: Transport to laboratory at 2-8 degrees C as soon as possible. NORMAL TEST CPT VALUE METHOD CODES By report Culture Respiratory Syncytial Virus Adenovirus Enteroviruses Herpes Screen VI22 Tissue Culture Isolation SPECIMEN: Nasopharyngeal swab, Nasal wash, Throat swab, VI22 Tissue Culture Isolation, Typing SPECIMEN: Nasopharyngeal swab, Nasal wash, Throat swab, VI22 Tissue Culture Isolation, Typing SPECIMEN: CSF, Stool, Throat swab CONTAINER: See virology specimen collection guide. VI24 Tissue Culture Isolation, Typing SPECIMEN: Lesion swab CONTAINER: See virology specimen collection guide. CONDITIONS: See virology specimen guide for specific Culture Culture Culture Culture Influenza VI22 Tissue Culture Isolation, Typing SPECIMEN: Nasopharyngeal swab, Nasal wash, Throat swab, Culture 87252
6 Parainfluenza VI22 Tissue Culture Isolation, Typing SPECIMEN: Nasopharyngeal swab, Nasal wash< Throat swab, NORMAL TEST CPT VALUE METHOD CODES Culture Varicella-Zoster VI22 Tissue Culture Isolation SPECIMEN: Vesicle fluid or scraping CONTAINER: See virology specimen collection guide. Culture Viral ID, Rabies VI32 Direct Fluorescent Antibody SPECIMEN: Freshly severed animal head, delivered by Animal Care Services or fresh unpreserved animal brain. (no formalin) CONTAINER: Any sterile transport container. COLLECTION: Remove brain from cranium of suspected animal, do not place in formalin. CONDITIONS: Transport to laboratory on wet ice or refrigerated, within 24 hrs. FITC Anti- Rabies Centocor Visceral Larval Migrans Antibody, (Toxocara) OI 44 Sent To Reference Lab. Additional information required. Please contact laboratory Send Out Yersinia Antibody OI 47 Sent To Reference Lab. Additional information required. Please contact laboratory Frozen:-20 C,serum only Send Out 99001
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